Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61634.t1 | XP_024638296.1 | 96.2 | 157 | 3 | 1 | 1 | 157 | 1 | 154 | 1.10E-77 | 299.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61634.t1 | Q54SW1 | 28.2 | 142 | 89 | 3 | 9 | 144 | 6 | 140 | 1.4e-09 | 64.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61634.t1 | I3SJM8 | 96.2 | 157 | 3 | 1 | 1 | 157 | 1 | 154 | 7.6e-78 | 299.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene46429 | MS.gene61634 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61634.t1 | MTR_4g050400 | 92.308 | 130 | 7 | 1 | 1 | 130 | 1 | 127 | 5.94e-69 | 206 |
| MS.gene61634.t1 | MTR_1g055425 | 56.000 | 125 | 55 | 0 | 33 | 157 | 31 | 155 | 7.81e-48 | 152 |
| MS.gene61634.t1 | MTR_1g055435 | 56.000 | 125 | 55 | 0 | 33 | 157 | 32 | 156 | 9.08e-45 | 144 |
| MS.gene61634.t1 | MTR_5g085900 | 43.066 | 137 | 75 | 2 | 24 | 157 | 26 | 162 | 1.99e-34 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61634.t1 | AT3G44100 | 61.871 | 139 | 49 | 2 | 16 | 154 | 15 | 149 | 4.37e-55 | 171 |
| MS.gene61634.t1 | AT3G11780 | 50.676 | 148 | 72 | 1 | 10 | 157 | 7 | 153 | 1.31e-47 | 151 |
| MS.gene61634.t1 | AT5G06480 | 52.800 | 125 | 59 | 0 | 33 | 157 | 29 | 153 | 5.73e-44 | 142 |
| MS.gene61634.t1 | AT3G11780 | 45.181 | 166 | 72 | 2 | 10 | 157 | 7 | 171 | 2.96e-43 | 141 |
Find 45 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGCAGGTAAAGCTCTTTA+TGG | 0.233971 | 4.1:+47503715 | MS.gene61634:intron |
| TAAATTTAATGCAAGTTAAC+TGG | 0.302105 | 4.1:-47510107 | None:intergenic |
| TATGGCGGAGATGTGGTAAT+TGG | 0.323192 | 4.1:+47503733 | MS.gene61634:CDS |
| TAATTGGAGTTTCCTATGTT+GGG | 0.358950 | 4.1:+47503749 | MS.gene61634:CDS |
| TTTAAGATCTCAGCTACTTC+TGG | 0.359491 | 4.1:+47502952 | MS.gene61634:CDS |
| GGAGAGAAGAAGAAAGAGAT+TGG | 0.382690 | 4.1:-47502545 | None:intergenic |
| ACAAAATTTCCATTAGCAAC+TGG | 0.385673 | 4.1:-47503820 | None:intergenic |
| AGTGTCATCTTTAGAGCATA+AGG | 0.394451 | 4.1:-47510066 | None:intergenic |
| GTATCCTGTCCAGTTGCTAA+TGG | 0.399485 | 4.1:+47503811 | MS.gene61634:CDS |
| AGAAGTAGCTGAGATCTTAA+AGG | 0.414793 | 4.1:-47502950 | None:intergenic |
| GCACCACTGACAACAGGGTT+TGG | 0.414915 | 4.1:-47502919 | None:intergenic |
| TCAATTCCAGTCACCTTCAC+AGG | 0.422087 | 4.1:-47502889 | None:intergenic |
| TGAAAGGAAGAGGGTGGAGA+AGG | 0.431347 | 4.1:-47502566 | None:intergenic |
| GTAATTGGAGTTTCCTATGT+TGG | 0.445028 | 4.1:+47503748 | MS.gene61634:CDS |
| GAGTAATTGCAGGTAGTGTC+TGG | 0.460782 | 4.1:-47503855 | None:intergenic |
| TACTTCCTTACACAGATCAA+TGG | 0.478210 | 4.1:-47503791 | None:intergenic |
| ATCTGTGTATGTAGATAAGA+AGG | 0.478616 | 4.1:+47502858 | MS.gene61634:intron |
| GACTACCCTGTGAAGGTGAC+TGG | 0.487152 | 4.1:+47502883 | MS.gene61634:CDS |
| GAGAGAAGAAGAAAGAGATT+GGG | 0.492297 | 4.1:-47502544 | None:intergenic |
| TGAAACCATTGATCTGTGTA+AGG | 0.498467 | 4.1:+47503786 | MS.gene61634:CDS |
| AGCTGAGATCTTAAAGGTGG+CGG | 0.501221 | 4.1:-47502944 | None:intergenic |
| CGTGAAGAGATGAAAGGAAG+AGG | 0.512746 | 4.1:-47502576 | None:intergenic |
| ATTTCCATTAGCAACTGGAC+AGG | 0.518555 | 4.1:-47503815 | None:intergenic |
| AAGAGATGAAAGGAAGAGGG+TGG | 0.520636 | 4.1:-47502572 | None:intergenic |
| ACAGATCAATGGTTTCAGAA+TGG | 0.526306 | 4.1:-47503780 | None:intergenic |
| GCTGAGATCTTAAAGGTGGC+GGG | 0.538168 | 4.1:-47502943 | None:intergenic |
| ATGGACAGGCACCCCAACAT+AGG | 0.561157 | 4.1:-47503761 | None:intergenic |
| GTGAAGAGATGAAAGGAAGA+GGG | 0.569312 | 4.1:-47502575 | None:intergenic |
| TGCTCTAAAGATGACACTGA+AGG | 0.572941 | 4.1:+47510071 | MS.gene61634:CDS |
| AAAGTTACTTTCAAATATTG+CGG | 0.585934 | 4.1:+47502607 | MS.gene61634:CDS |
| AGCTCTTTATGGCGGAGATG+TGG | 0.589630 | 4.1:+47503726 | MS.gene61634:CDS |
| CTAACCGGTGGAGTAATTGC+AGG | 0.597019 | 4.1:-47503865 | None:intergenic |
| AATTGGAGTTTCCTATGTTG+GGG | 0.597198 | 4.1:+47503750 | MS.gene61634:CDS |
| TGAGATCTTAAAGGTGGCGG+GGG | 0.598409 | 4.1:-47502941 | None:intergenic |
| CTTGAACGTGAAGAGATGAA+AGG | 0.605785 | 4.1:-47502582 | None:intergenic |
| CAATTCCAGTCACCTTCACA+GGG | 0.611266 | 4.1:-47502888 | None:intergenic |
| GAAGGCTGACTACCCTGTGA+AGG | 0.619896 | 4.1:+47502876 | MS.gene61634:CDS |
| CTGAGATCTTAAAGGTGGCG+GGG | 0.624735 | 4.1:-47502942 | None:intergenic |
| ACTACCTGCAATTACTCCAC+CGG | 0.625559 | 4.1:+47503861 | MS.gene61634:CDS |
| TCAATGGTTTCAGAATGGAC+AGG | 0.634798 | 4.1:-47503775 | None:intergenic |
| TTACCAAACCCTGTTGTCAG+TGG | 0.649255 | 4.1:+47502916 | MS.gene61634:CDS |
| GATGACACTGAAGGATGACA+AGG | 0.657728 | 4.1:+47510080 | MS.gene61634:CDS |
| AGTAGCTGAGATCTTAAAGG+TGG | 0.675679 | 4.1:-47502947 | None:intergenic |
| GCAGGTAAAGCTCTTTATGG+CGG | 0.686450 | 4.1:+47503718 | MS.gene61634:intron |
| ATGACACTGAAGGATGACAA+GGG | 0.715369 | 4.1:+47510081 | MS.gene61634:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 47502526 | 47510170 | 47502526 | ID=MS.gene61634 |
| chr4.1 | mRNA | 47502526 | 47510170 | 47502526 | ID=MS.gene61634.t1;Parent=MS.gene61634 |
| chr4.1 | exon | 47502526 | 47502628 | 47502526 | ID=MS.gene61634.t1.exon1;Parent=MS.gene61634.t1 |
| chr4.1 | CDS | 47502526 | 47502628 | 47502526 | ID=cds.MS.gene61634.t1;Parent=MS.gene61634.t1 |
| chr4.1 | exon | 47502872 | 47502973 | 47502872 | ID=MS.gene61634.t1.exon2;Parent=MS.gene61634.t1 |
| chr4.1 | CDS | 47502872 | 47502973 | 47502872 | ID=cds.MS.gene61634.t1;Parent=MS.gene61634.t1 |
| chr4.1 | exon | 47503722 | 47503882 | 47503722 | ID=MS.gene61634.t1.exon3;Parent=MS.gene61634.t1 |
| chr4.1 | CDS | 47503722 | 47503882 | 47503722 | ID=cds.MS.gene61634.t1;Parent=MS.gene61634.t1 |
| chr4.1 | exon | 47510063 | 47510170 | 47510063 | ID=MS.gene61634.t1.exon4;Parent=MS.gene61634.t1 |
| chr4.1 | CDS | 47510063 | 47510170 | 47510063 | ID=cds.MS.gene61634.t1;Parent=MS.gene61634.t1 |
| Gene Sequence |
| Protein sequence |
