AlfalfaGEDB | MS.gene61641

M. sativa cultivar XinJiangDaYe / MS.gene61641

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61641.t1	XP_003605992.2	94.3	140	8	0	1	140	1	140	6.80E-52	213.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61641.t1	Q9SHS7	63.7	146	46	2	1	140	1	145	4.2e-26	119.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61641.t1	G7JET4	94.3	140	8	0	1	140	1	140	4.9e-52	213.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene059007	MS.gene61641	0.811051	8.49E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61641.t1	MTR_4g050540	96.429	140	5	0	1	140	1	140	2.67e-85	268
MS.gene61641.t1	MTR_2g087530	67.808	146	30	2	1	140	1	135	5.56e-52	176
MS.gene61641.t1	MTR_1g089370	54.098	61	22	1	69	129	10	64	4.59e-14	68.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61641.t1	AT1G08420	56.173	162	43	5	1	140	1	156	2.10e-44	155
MS.gene61641.t1	AT1G08420	56.173	162	43	5	1	140	1	156	2.22e-44	155
MS.gene61641.t1	AT2G27210	60.000	145	53	1	1	140	1	145	1.63e-42	149
MS.gene61641.t1	AT2G27210	60.000	145	53	1	1	140	1	145	1.81e-42	149
MS.gene61641.t1	AT4G03080	51.667	60	23	1	69	128	10	63	6.54e-13	65.1

Find 59 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TACAGTTGTGGATGCGATTT+TGG	0.192831	4.1:-47354949	MS.gene61641:CDS
GCGCCGGCGCCGTCGTCTTC+CGG	0.319623	4.1:-47355068	MS.gene61641:CDS
GGCTGTGCCGGCGGTGGGAG+AGG	0.337377	4.1:-47354880	MS.gene61641:CDS
GCAAGGTGCTTCGGCTGGTC+AGG	0.354401	4.1:-47355015	MS.gene61641:CDS
ATTGAAGATAGAGAACAATT+AGG	0.363504	4.1:-47355107	MS.gene61641:CDS
ATTAGGAGGGAATTCGATTC+CGG	0.380600	4.1:-47355090	MS.gene61641:CDS
TGCTGTGATTGTTGCTCCGC+CGG	0.385383	4.1:+47355038	None:intergenic
GAAGGGAATTCAGCGGCGTC+AGG	0.392486	4.1:-47354798	MS.gene61641:CDS
GAAGGTACGCCGGGGTATAT+TGG	0.397267	4.1:-47354858	MS.gene61641:CDS
GGTGGTGCGACTGCGCTTGA+AGG	0.400535	4.1:-47354816	MS.gene61641:CDS
GTGGGAGAGGAAGGTACGCC+GGG	0.406102	4.1:-47354867	MS.gene61641:CDS
TCAGGGACTCCTACTTCTGC+TGG	0.441047	4.1:-47354780	MS.gene61641:CDS
GTGCAGCATAGTTCGGTTGT+TGG	0.448434	4.1:-47354993	MS.gene61641:CDS
GTCGTCTTCCGGTGGATCTC+CGG	0.449558	4.1:-47355057	MS.gene61641:CDS
ATCACAGCAGCAAGGTGCTT+CGG	0.463264	4.1:-47355024	MS.gene61641:CDS
GTGCCGGCGGTGGGAGAGGA+AGG	0.465868	4.1:-47354876	MS.gene61641:CDS
AGGAAGATGGACCGGGGCCG+AGG	0.468813	4.1:-47354920	MS.gene61641:CDS
GTTGACGGCTGTGCCGGCGG+TGG	0.479291	4.1:-47354886	MS.gene61641:CDS
CCTACTTCTGCTGGAAATGC+AGG	0.480415	4.1:-47354771	MS.gene61641:CDS
AGGGAATTCGATTCCGGCGC+CGG	0.484972	4.1:-47355084	MS.gene61641:CDS
GGAGAAGAAGGAAGATGGAC+CGG	0.490147	4.1:-47354928	MS.gene61641:CDS
GAAGACGACGGCGCCGGCGC+CGG	0.494181	4.1:+47355071	None:intergenic
TGGTCAGGTGCAGCATAGTT+CGG	0.496759	4.1:-47355000	MS.gene61641:CDS
CAGCAGCAAGGTGCTTCGGC+TGG	0.500950	4.1:-47355020	MS.gene61641:CDS
GAAGATAGAGAACAATTAGG+AGG	0.505302	4.1:-47355104	MS.gene61641:CDS
TCATACGTTGACGGCTGTGC+CGG	0.516746	4.1:-47354892	MS.gene61641:CDS
CCACCGGAAGACGACGGCGC+CGG	0.519033	4.1:+47355065	None:intergenic
GATGGACCGGGGCCGAGGTG+TGG	0.525041	4.1:-47354915	MS.gene61641:CDS
GAGAAGAAGGAAGATGGACC+GGG	0.526516	4.1:-47354927	MS.gene61641:CDS
GTGGTGCGACTGCGCTTGAA+GGG	0.527943	4.1:-47354815	MS.gene61641:CDS
TTGCTCCGCCGGAGATCCAC+CGG	0.529045	4.1:+47355049	None:intergenic
AAGATAGAGAACAATTAGGA+GGG	0.539534	4.1:-47355103	MS.gene61641:CDS
TTGACGGCTGTGCCGGCGGT+GGG	0.546220	4.1:-47354885	MS.gene61641:CDS
TGTTGATTCATCGATGGCAT+CGG	0.563011	4.1:-47355159	MS.gene61641:CDS
CCGGCGCCGTCGTCTTCCGG+TGG	0.568390	4.1:-47355065	MS.gene61641:CDS
CCTGCATTTCCAGCAGAAGT+AGG	0.569856	4.1:+47354771	None:intergenic
GGTGGGAGAGGAAGGTACGC+CGG	0.572703	4.1:-47354868	MS.gene61641:CDS
GTATAACTCGGCGCTAGCCT+CGG	0.574774	4.1:+47354969	None:intergenic
TACGTTGACGGCTGTGCCGG+CGG	0.576135	4.1:-47354889	MS.gene61641:CDS
GTATGACCACACCTCGGCCC+CGG	0.577475	4.1:+47354909	None:intergenic
AAGGGAATTCAGCGGCGTCA+GGG	0.592219	4.1:-47354797	MS.gene61641:CDS
GTACCTTCCTCTCCCACCGC+CGG	0.599932	4.1:+47354873	None:intergenic
CCACCGAACAAAATCAGCCT+CGG	0.614940	4.1:+47354834	None:intergenic
AATGGATGTTGATTCATCGA+TGG	0.615102	4.1:-47355165	MS.gene61641:CDS
GAGCAACAATCACAGCAGCA+AGG	0.615133	4.1:-47355032	MS.gene61641:CDS
GAGGTGTGGTCATACGTTGA+CGG	0.633733	4.1:-47354901	MS.gene61641:CDS
GTCTTCCGGTGGATCTCCGG+CGG	0.636500	4.1:-47355054	MS.gene61641:CDS
AGCGCCGAGTTATACAGTTG+TGG	0.637608	4.1:-47354961	MS.gene61641:CDS
GGAGATCCACCGGAAGACGA+CGG	0.641122	4.1:+47355059	None:intergenic
ATAGTTCGGTTGTTGGACCG+AGG	0.659224	4.1:-47354986	MS.gene61641:CDS
CGCCGGGGTATATTGGTCCG+AGG	0.664294	4.1:-47354851	MS.gene61641:CDS
GCATCCACAACTGTATAACT+CGG	0.668385	4.1:+47354957	None:intergenic
TGCGCTTGAAGGGAATTCAG+CGG	0.675144	4.1:-47354805	MS.gene61641:CDS
GAATGATAACGACTCAACGG+TGG	0.675250	4.1:-47355135	MS.gene61641:CDS
AGCCTCGGACCAATATACCC+CGG	0.689572	4.1:+47354849	None:intergenic
GGAGAATGATAACGACTCAA+CGG	0.690770	4.1:-47355138	MS.gene61641:CDS
TGGGAGAGGAAGGTACGCCG+GGG	0.711923	4.1:-47354866	MS.gene61641:CDS
AGAAGAAGGAAGATGGACCG+GGG	0.715571	4.1:-47354926	MS.gene61641:CDS
GTCAACGTATGACCACACCT+CGG	0.753346	4.1:+47354903	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATTGAAGATAGAGAACAATT+AGG	-	chr4.1:47354823-47354842	MS.gene61641:CDS	25.0%
	AAGATAGAGAACAATTAGGA+GGG	-	chr4.1:47354827-47354846	MS.gene61641:CDS	30.0%
	GAAGATAGAGAACAATTAGG+AGG	-	chr4.1:47354826-47354845	MS.gene61641:CDS	35.0%
!!	ATTTTGGAGAAGAAGGAAGA+TGG	-	chr4.1:47354997-47355016	MS.gene61641:CDS	35.0%
	ATTAGGAGGGAATTCGATTC+CGG	-	chr4.1:47354840-47354859	MS.gene61641:CDS	40.0%
	GCATCCACAACTGTATAACT+CGG	+	chr4.1:47354976-47354995	None:intergenic	40.0%
!	GGAGAATGATAACGACTCAA+CGG	-	chr4.1:47354792-47354811	MS.gene61641:CDS	40.0%
!	TACAGTTGTGGATGCGATTT+TGG	-	chr4.1:47354981-47355000	MS.gene61641:CDS	40.0%
!!	TGTTGATTCATCGATGGCAT+CGG	-	chr4.1:47354771-47354790	MS.gene61641:CDS	40.0%
!	GAATGATAACGACTCAACGG+TGG	-	chr4.1:47354795-47354814	MS.gene61641:CDS	45.0%
!!	GGATGCGATTTTGGAGAAGA+AGG	-	chr4.1:47354990-47355009	MS.gene61641:CDS	45.0%
	AGAAGAAGGAAGATGGACCG+GGG	-	chr4.1:47355004-47355023	MS.gene61641:CDS	50.0%
	ATAGTTCGGTTGTTGGACCG+AGG	-	chr4.1:47354944-47354963	MS.gene61641:CDS	50.0%
	CCACCGAACAAAATCAGCCT+CGG	+	chr4.1:47355099-47355118	None:intergenic	50.0%
	CCTACTTCTGCTGGAAATGC+AGG	-	chr4.1:47355159-47355178	MS.gene61641:CDS	50.0%
	CCTGCATTTCCAGCAGAAGT+AGG	+	chr4.1:47355162-47355181	None:intergenic	50.0%
	GAGAAGAAGGAAGATGGACC+GGG	-	chr4.1:47355003-47355022	MS.gene61641:CDS	50.0%
	GAGCAACAATCACAGCAGCA+AGG	-	chr4.1:47354898-47354917	MS.gene61641:CDS	50.0%
	GAGGTGTGGTCATACGTTGA+CGG	-	chr4.1:47355029-47355048	MS.gene61641:CDS	50.0%
	GGAGAAGAAGGAAGATGGAC+CGG	-	chr4.1:47355002-47355021	MS.gene61641:CDS	50.0%
	GTCAACGTATGACCACACCT+CGG	+	chr4.1:47355030-47355049	None:intergenic	50.0%
	GTGCAGCATAGTTCGGTTGT+TGG	-	chr4.1:47354937-47354956	MS.gene61641:CDS	50.0%
	TGCGCTTGAAGGGAATTCAG+CGG	-	chr4.1:47355125-47355144	MS.gene61641:CDS	50.0%
	TGGTCAGGTGCAGCATAGTT+CGG	-	chr4.1:47354930-47354949	MS.gene61641:CDS	50.0%
!	AGCGCCGAGTTATACAGTTG+TGG	-	chr4.1:47354969-47354988	MS.gene61641:CDS	50.0%
!	ATCACAGCAGCAAGGTGCTT+CGG	-	chr4.1:47354906-47354925	MS.gene61641:CDS	50.0%
!!	GGTCCGAGGCTGATTTTGTT+CGG	-	chr4.1:47355093-47355112	MS.gene61641:CDS	50.0%
	AAGGGAATTCAGCGGCGTCA+GGG	-	chr4.1:47355133-47355152	MS.gene61641:CDS	55.0%
	AGCCTCGGACCAATATACCC+CGG	+	chr4.1:47355084-47355103	None:intergenic	55.0%
	TCAGGGACTCCTACTTCTGC+TGG	-	chr4.1:47355150-47355169	MS.gene61641:CDS	55.0%
	TCATACGTTGACGGCTGTGC+CGG	-	chr4.1:47355038-47355057	MS.gene61641:CDS	55.0%
	TGCTGTGATTGTTGCTCCGC+CGG	+	chr4.1:47354895-47354914	None:intergenic	55.0%
!	GAAGGTACGCCGGGGTATAT+TGG	-	chr4.1:47355072-47355091	MS.gene61641:CDS	55.0%
!	GTATAACTCGGCGCTAGCCT+CGG	+	chr4.1:47354964-47354983	None:intergenic	55.0%
!!	CCGAGGCTGATTTTGTTCGG+TGG	-	chr4.1:47355096-47355115	MS.gene61641:CDS	55.0%
	AGGGAATTCGATTCCGGCGC+CGG	-	chr4.1:47354846-47354865	MS.gene61641:CDS	60.0%
	GAAGGGAATTCAGCGGCGTC+AGG	-	chr4.1:47355132-47355151	MS.gene61641:CDS	60.0%
	GGAGATCCACCGGAAGACGA+CGG	+	chr4.1:47354874-47354893	None:intergenic	60.0%
	GTCGTCTTCCGGTGGATCTC+CGG	-	chr4.1:47354873-47354892	MS.gene61641:CDS	60.0%
	GTGGTGCGACTGCGCTTGAA+GGG	-	chr4.1:47355115-47355134	MS.gene61641:CDS	60.0%
	CGCCGGGGTATATTGGTCCG+AGG	-	chr4.1:47355079-47355098	MS.gene61641:CDS	65.0%
	GGTGGTGCGACTGCGCTTGA+AGG	-	chr4.1:47355114-47355133	MS.gene61641:CDS	65.0%
	GTACCTTCCTCTCCCACCGC+CGG	+	chr4.1:47355060-47355079	None:intergenic	65.0%
	GTATGACCACACCTCGGCCC+CGG	+	chr4.1:47355024-47355043	None:intergenic	65.0%
	GTCTTCCGGTGGATCTCCGG+CGG	-	chr4.1:47354876-47354895	MS.gene61641:CDS	65.0%
	TACGTTGACGGCTGTGCCGG+CGG	-	chr4.1:47355041-47355060	MS.gene61641:CDS	65.0%
	TTGCTCCGCCGGAGATCCAC+CGG	+	chr4.1:47354884-47354903	None:intergenic	65.0%
!	CAGCAGCAAGGTGCTTCGGC+TGG	-	chr4.1:47354910-47354929	MS.gene61641:CDS	65.0%
!	GCAAGGTGCTTCGGCTGGTC+AGG	-	chr4.1:47354915-47354934	MS.gene61641:CDS	65.0%
!	GGTGGGAGAGGAAGGTACGC+CGG	-	chr4.1:47355062-47355081	MS.gene61641:CDS	65.0%
!	GTGGGAGAGGAAGGTACGCC+GGG	-	chr4.1:47355063-47355082	MS.gene61641:CDS	65.0%
!	TGGGAGAGGAAGGTACGCCG+GGG	-	chr4.1:47355064-47355083	MS.gene61641:CDS	65.0%
	AGGAAGATGGACCGGGGCCG+AGG	-	chr4.1:47355010-47355029	MS.gene61641:CDS	70.0%
!	TTGACGGCTGTGCCGGCGGT+GGG	-	chr4.1:47355045-47355064	MS.gene61641:CDS	70.0%
	CCACCGGAAGACGACGGCGC+CGG	+	chr4.1:47354868-47354887	None:intergenic	75.0%
	GATGGACCGGGGCCGAGGTG+TGG	-	chr4.1:47355015-47355034	MS.gene61641:CDS	75.0%
!	GTGCCGGCGGTGGGAGAGGA+AGG	-	chr4.1:47355054-47355073	MS.gene61641:CDS	75.0%
!	GTTGACGGCTGTGCCGGCGG+TGG	-	chr4.1:47355044-47355063	MS.gene61641:CDS	75.0%
!	CCGGCGCCGTCGTCTTCCGG+TGG	-	chr4.1:47354865-47354884	MS.gene61641:CDS	80.0%
!!	GCGCCGGCGCCGTCGTCTTC+CGG	-	chr4.1:47354862-47354881	MS.gene61641:CDS	80.0%
!!	GGCTGTGCCGGCGGTGGGAG+AGG	-	chr4.1:47355050-47355069	MS.gene61641:CDS	80.0%
!!!	GAAGACGACGGCGCCGGCGC+CGG	+	chr4.1:47354862-47354881	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	47354766	47355186	47354766	ID=MS.gene61641
chr4.1	mRNA	47354766	47355186	47354766	ID=MS.gene61641.t1;Parent=MS.gene61641
chr4.1	exon	47354766	47355186	47354766	ID=MS.gene61641.t1.exon1;Parent=MS.gene61641.t1
chr4.1	CDS	47354766	47355186	47354766	ID=cds.MS.gene61641.t1;Parent=MS.gene61641.t1

Gene Sequence

>MS.gene61641

ATGGATGTTGATTCATCGATGGCATCGGAGAATGATAACGACTCAACGGTGGCGAAAATTGAAGATAGAGAACAATTAGGAGGGAATTCGATTCCGGCGCCGGCGCCGTCGTCTTCCGGTGGATCTCCGGCGGAGCAACAATCACAGCAGCAAGGTGCTTCGGCTGGTCAGGTGCAGCATAGTTCGGTTGTTGGACCGAGGCTAGCGCCGAGTTATACAGTTGTGGATGCGATTTTGGAGAAGAAGGAAGATGGACCGGGGCCGAGGTGTGGTCATACGTTGACGGCTGTGCCGGCGGTGGGAGAGGAAGGTACGCCGGGGTATATTGGTCCGAGGCTGATTTTGTTCGGTGGTGCGACTGCGCTTGAAGGGAATTCAGCGGCGTCAGGGACTCCTACTTCTGCTGGAAATGCAGGCATAC

Protein sequence

>MS.gene61641.t1

MDVDSSMASENDNDSTVAKIEDREQLGGNSIPAPAPSSSGGSPAEQQSQQQGASAGQVQHSSVVGPRLAPSYTVVDAILEKKEDGPGPRCGHTLTAVPAVGEEGTPGYIGPRLILFGGATALEGNSAASGTPTSAGNAGI