Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61724.t1 | XP_003609735.1 | 94.2 | 360 | 21 | 0 | 1 | 360 | 16 | 375 | 3.10E-197 | 697.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61724.t1 | A0A1C9CX66 | 41.8 | 361 | 199 | 7 | 1 | 357 | 37 | 390 | 6.9e-73 | 275.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61724.t1 | G7JMR7 | 94.2 | 360 | 21 | 0 | 1 | 360 | 16 | 375 | 2.2e-197 | 697.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054146 | MS.gene61724 | 0.80594 | 1.05E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61724.t1 | MTR_4g121570 | 93.906 | 361 | 22 | 0 | 1 | 361 | 16 | 376 | 0.0 | 711 |
| MS.gene61724.t1 | MTR_4g073090 | 83.380 | 361 | 60 | 0 | 1 | 361 | 16 | 376 | 0.0 | 648 |
| MS.gene61724.t1 | MTR_4g121590 | 81.440 | 361 | 67 | 0 | 1 | 361 | 16 | 376 | 0.0 | 629 |
| MS.gene61724.t1 | MTR_3g102730 | 41.734 | 369 | 188 | 9 | 1 | 357 | 37 | 390 | 3.21e-92 | 281 |
| MS.gene61724.t1 | MTR_3g102770 | 42.308 | 364 | 192 | 9 | 1 | 357 | 37 | 389 | 3.60e-91 | 278 |
| MS.gene61724.t1 | MTR_3g102760 | 41.713 | 362 | 198 | 9 | 1 | 357 | 37 | 390 | 3.67e-91 | 278 |
| MS.gene61724.t1 | MTR_3g013890 | 41.192 | 369 | 190 | 9 | 1 | 357 | 37 | 390 | 5.78e-91 | 278 |
| MS.gene61724.t1 | MTR_1g018590 | 40.278 | 360 | 205 | 7 | 1 | 357 | 42 | 394 | 1.08e-86 | 267 |
| MS.gene61724.t1 | MTR_1g018610 | 40.443 | 361 | 203 | 8 | 1 | 357 | 71 | 423 | 9.07e-86 | 266 |
| MS.gene61724.t1 | MTR_1g018580 | 38.056 | 360 | 189 | 7 | 1 | 357 | 36 | 364 | 7.28e-77 | 241 |
| MS.gene61724.t1 | MTR_3g463660 | 35.870 | 368 | 214 | 12 | 1 | 357 | 19 | 375 | 3.65e-59 | 196 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61724.t1 | AT4G24220 | 41.873 | 363 | 194 | 8 | 1 | 357 | 37 | 388 | 7.97e-91 | 278 |
| MS.gene61724.t1 | AT4G24220 | 41.873 | 363 | 194 | 8 | 1 | 357 | 36 | 387 | 1.11e-90 | 277 |
| MS.gene61724.t1 | AT5G58750 | 30.978 | 368 | 234 | 11 | 1 | 357 | 19 | 377 | 4.53e-52 | 177 |
| MS.gene61724.t1 | AT5G58750 | 30.978 | 368 | 234 | 11 | 1 | 357 | 27 | 385 | 5.54e-52 | 177 |
Find 0 sgRNAs with CRISPR-Local
Find 108 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAATAAGAGTAAAGATTA+TGG | + | 32467:977-996 | MS.gene61724:CDS | 20.0% |
| !! | TGAATAAGAGTAAAGATTAT+GGG | + | 32467:978-997 | MS.gene61724:CDS | 20.0% |
| ! | CAAGCATTTAATTGTACAAA+CGG | + | 32467:719-738 | MS.gene61724:CDS | 25.0% |
| AAATTTCTACTACACACTTG+AGG | + | 32467:442-461 | MS.gene61724:CDS | 30.0% | |
| CAAGTGTGTAGTAGAAATTT+GGG | - | 32467:442-461 | MS.gene61724:intergenic | 30.0% | |
| CGTTTGTACAATTAAATGCT+TGG | - | 32467:721-740 | MS.gene61724:intergenic | 30.0% | |
| GACTTGATTGTAGAGAAATA+TGG | + | 32467:869-888 | MS.gene61724:CDS | 30.0% | |
| TCAAGTGTGTAGTAGAAATT+TGG | - | 32467:443-462 | MS.gene61724:intergenic | 30.0% | |
| TGTAGTAGAAATTTGGGTAA+GGG | - | 32467:436-455 | MS.gene61724:intergenic | 30.0% | |
| TGTATTGAGTATGTTATCGA+AGG | - | 32467:488-507 | MS.gene61724:intergenic | 30.0% | |
| !! | GTTTAAGAGTATCAAACGTT+GGG | + | 32467:1021-1040 | MS.gene61724:CDS | 30.0% |
| AACCGGAACTAAACATTACA+TGG | + | 32467:334-353 | MS.gene61724:CDS | 35.0% | |
| ACCGGAACTAAACATTACAT+GGG | + | 32467:335-354 | MS.gene61724:CDS | 35.0% | |
| GTGTAGTAGAAATTTGGGTA+AGG | - | 32467:437-456 | MS.gene61724:intergenic | 35.0% | |
| GTTGGCTATAGGTAATAGTT+TGG | - | 32467:171-190 | MS.gene61724:intergenic | 35.0% | |
| ! | ACCCATGTAATGTTTAGTTC+CGG | - | 32467:339-358 | MS.gene61724:intergenic | 35.0% |
| ! | ACTTCATAAGACCAAGTTGA+CGG | + | 32467:892-911 | MS.gene61724:CDS | 35.0% |
| ! | TGTAATGTTTAGTTCCGGTT+TGG | - | 32467:334-353 | MS.gene61724:intergenic | 35.0% |
| !! | CGTTTAAGAGTATCAAACGT+TGG | + | 32467:1020-1039 | MS.gene61724:CDS | 35.0% |
| !! | GACATATAAGTTGGTTAGAC+CGG | - | 32467:379-398 | MS.gene61724:intergenic | 35.0% |
| !! | TGATGTTGAGTTTGTTGAGT+TGG | + | 32467:790-809 | MS.gene61724:CDS | 35.0% |
| !! | TTTTCACTTGGAAGAGTATG+TGG | + | 32467:747-766 | MS.gene61724:CDS | 35.0% |
| !!! | TATGGGTTTTTTGAGTATGC+TGG | + | 32467:995-1014 | MS.gene61724:CDS | 35.0% |
| ACCCAGGAAATAGATACACT+TGG | + | 32467:615-634 | MS.gene61724:CDS | 40.0% | |
| AGATGGGGAAGATTTAAGGA+CGG | - | 32467:288-307 | MS.gene61724:intergenic | 40.0% | |
| CACAACCGTAAACTTTCCAT+GGG | - | 32467:61-80 | MS.gene61724:intergenic | 40.0% | |
| CATCGATCCTCTCTCATAAT+AGG | + | 32467:506-525 | MS.gene61724:CDS | 40.0% | |
| CCCAAGTGTATCTATTTCCT+GGG | - | 32467:619-638 | MS.gene61724:intergenic | 40.0% | |
| CCCAGGAAATAGATACACTT+GGG | + | 32467:616-635 | MS.gene61724:CDS | 40.0% | |
| CGAGGCATATTCTCGTTAAA+AGG | - | 32467:410-429 | MS.gene61724:intergenic | 40.0% | |
| CTTCTTCATCAGCTACAAAC+TGG | - | 32467:223-242 | MS.gene61724:intergenic | 40.0% | |
| GAGAAGATGGGGAAGATTTA+AGG | - | 32467:292-311 | MS.gene61724:intergenic | 40.0% | |
| GGCATATTCTCGTTAAAAGG+TGG | - | 32467:407-426 | MS.gene61724:intergenic | 40.0% | |
| GTGGTTCGTGACATATAAGT+TGG | - | 32467:388-407 | MS.gene61724:intergenic | 40.0% | |
| TCCCAAGTGTATCTATTTCC+TGG | - | 32467:620-639 | MS.gene61724:intergenic | 40.0% | |
| TGAAACTCAAACCGTACAAC+TGG | - | 32467:944-963 | MS.gene61724:intergenic | 40.0% | |
| TTCCCTTCTTCCATCTTAGA+CGG | + | 32467:104-123 | MS.gene61724:CDS | 40.0% | |
| !! | AATTGCTTGCTTTGAAGCCA+TGG | + | 32467:916-935 | MS.gene61724:CDS | 40.0% |
| !! | GAGGAGCATGGATTTGTTAA+CGG | - | 32467:255-274 | MS.gene61724:intergenic | 40.0% |
| !! | TAAGAGTATCAAACGTTGGG+TGG | + | 32467:1024-1043 | MS.gene61724:CDS | 40.0% |
| AAATTTGGGTAAGGGAGTCG+AGG | - | 32467:428-447 | MS.gene61724:intergenic | 45.0% | |
| AGACGCACCTATTATGAGAG+AGG | - | 32467:516-535 | MS.gene61724:intergenic | 45.0% | |
| GCAAGCAATTTCCGTCAACT+TGG | - | 32467:906-925 | MS.gene61724:intergenic | 45.0% | |
| GCACAACCGTAAACTTTCCA+TGG | - | 32467:62-81 | MS.gene61724:intergenic | 45.0% | |
| GGTAATGAAGCCGTCTAAGA+TGG | - | 32467:117-136 | MS.gene61724:intergenic | 45.0% | |
| TAACCCTACATGGCGACATA+TGG | - | 32467:585-604 | MS.gene61724:intergenic | 45.0% | |
| TATGTCAGATGCAGATGTGC+TGG | + | 32467:652-671 | MS.gene61724:CDS | 45.0% | |
| TTCTCCTCTCGCCCATATAA+CGG | + | 32467:307-326 | MS.gene61724:CDS | 45.0% | |
| TTGAAGCAACCTGGTTGTCT+CGG | + | 32467:32-51 | MS.gene61724:CDS | 45.0% | |
| ! | AAGCCGTCTAAGATGGAAGA+AGG | - | 32467:110-129 | MS.gene61724:intergenic | 45.0% |
| ! | AGCCGTCTAAGATGGAAGAA+GGG | - | 32467:109-128 | MS.gene61724:intergenic | 45.0% |
| ! | CAAACGGCGACATTTTCACT+TGG | + | 32467:735-754 | MS.gene61724:CDS | 45.0% |
| !! | ATAAGTTGGTTAGACCGGAC+CGG | - | 32467:374-393 | MS.gene61724:intergenic | 45.0% |
| !! | CTATTTCCTGGGTACCGAAA+CGG | - | 32467:608-627 | MS.gene61724:intergenic | 45.0% |
| !! | GTTGAGTTGATGCGTGACAA+GGG | + | 32467:836-855 | MS.gene61724:CDS | 45.0% |
| !! | TCCGGTTTGGACCGTTATAT+GGG | - | 32467:321-340 | MS.gene61724:intergenic | 45.0% |
| !! | TTCCGGTTTGGACCGTTATA+TGG | - | 32467:322-341 | MS.gene61724:intergenic | 45.0% |
| AAACCGTACAACTGGCACCA+TGG | - | 32467:936-955 | MS.gene61723:intergenic | 50.0% | |
| ACCAGGTTGCTTCAAGGCTT+CGG | - | 32467:27-46 | MS.gene61724:intergenic | 50.0% | |
| ACCGAAACGGTAACCCTACA+TGG | - | 32467:595-614 | MS.gene61724:intergenic | 50.0% | |
| AGCTGAGTCAACTGCGTCAA+AGG | - | 32467:138-157 | MS.gene61724:intergenic | 50.0% | |
| ATATGGGCGAGAGGAGAAGA+TGG | - | 32467:305-324 | MS.gene61724:intergenic | 50.0% | |
| CATGGGTCCGATATATGACC+CGG | + | 32467:352-371 | MS.gene61724:CDS | 50.0% | |
| CTGCCATATGTCGCCATGTA+GGG | + | 32467:579-598 | MS.gene61724:CDS | 50.0% | |
| GAGACAACCAGGTTGCTTCA+AGG | - | 32467:33-52 | MS.gene61724:intergenic | 50.0% | |
| GCCATGTAGGGTTACCGTTT+CGG | + | 32467:591-610 | MS.gene61724:CDS | 50.0% | |
| GCCCATATAACGGTCCAAAC+CGG | + | 32467:317-336 | MS.gene61724:CDS | 50.0% | |
| GCTGGCAAAGCAACATGTGT+GGG | + | 32467:670-689 | MS.gene61724:CDS | 50.0% | |
| GGAGGCCCATGGAAAGTTTA+CGG | + | 32467:53-72 | MS.gene61724:CDS | 50.0% | |
| TAAGGACGGTGACAACGTTG+AGG | - | 32467:274-293 | MS.gene61724:intergenic | 50.0% | |
| TATGGGCGAGAGGAGAAGAT+GGG | - | 32467:304-323 | MS.gene61724:intergenic | 50.0% | |
| TGCTGGCAAAGCAACATGTG+TGG | + | 32467:669-688 | MS.gene61724:CDS | 50.0% | |
| ! | AAAGCAACATGTGTGGGCTG+CGG | + | 32467:676-695 | MS.gene61724:CDS | 50.0% |
| ! | AAGCCATGGTGCCAGTTGTA+CGG | + | 32467:930-949 | MS.gene61724:CDS | 50.0% |
| ! | TGGGTGACTTCGTTGGCTAT+AGG | - | 32467:182-201 | MS.gene61724:intergenic | 50.0% |
| !! | CGTTGAGTTGATGCGTGACA+AGG | + | 32467:835-854 | MS.gene61724:CDS | 50.0% |
| !! | TAAGTTGGTTAGACCGGACC+GGG | - | 32467:373-392 | MS.gene61724:intergenic | 50.0% |
| AACGTTGGGTGGCTAAGCTG+AGG | + | 32467:1035-1054 | MS.gene61724:CDS | 55.0% | |
| AAGAAGGGAACCAGCCCATG+GGG | - | 32467:94-113 | MS.gene61724:intergenic | 55.0% | |
| AAGCAACCTGGTTGTCTCGG+AGG | + | 32467:35-54 | MS.gene61724:CDS | 55.0% | |
| ACGAAGTCACCCACCTCTTC+TGG | + | 32467:189-208 | MS.gene61724:CDS | 55.0% | |
| ACGTTGGGTGGCTAAGCTGA+GGG | + | 32467:1036-1055 | MS.gene61724:CDS | 55.0% | |
| ACTGGAACGTCACCCAGAAG+AGG | - | 32467:205-224 | MS.gene61724:intergenic | 55.0% | |
| ATGGGCGAGAGGAGAAGATG+GGG | - | 32467:303-322 | MS.gene61724:intergenic | 55.0% | |
| CGAAGTCACCCACCTCTTCT+GGG | + | 32467:190-209 | MS.gene61724:CDS | 55.0% | |
| GAACGTCACCCAGAAGAGGT+GGG | - | 32467:201-220 | MS.gene61724:intergenic | 55.0% | |
| GAAGAAGGGAACCAGCCCAT+GGG | - | 32467:95-114 | MS.gene61724:intergenic | 55.0% | |
| GAAGAGGTGGGTGACTTCGT+TGG | - | 32467:189-208 | MS.gene61724:intergenic | 55.0% | |
| GACCGGACCGGGTCATATAT+CGG | - | 32467:362-381 | MS.gene61724:intergenic | 55.0% | |
| GCGTGACAAGGGTGAGGTTT+GGG | + | 32467:847-866 | MS.gene61724:CDS | 55.0% | |
| GCTGCCATATGTCGCCATGT+AGG | + | 32467:578-597 | MS.gene61724:CDS | 55.0% | |
| GGTGACAACGTTGAGGAGCA+TGG | - | 32467:267-286 | MS.gene61724:intergenic | 55.0% | |
| GTCCGATATATGACCCGGTC+CGG | + | 32467:357-376 | MS.gene61724:CDS | 55.0% | |
| GTTGATGCGTGACAAGGGTG+AGG | + | 32467:841-860 | MS.gene61724:CDS | 55.0% | |
| GTTGCTTCAAGGCTTCGGCT+AGG | - | 32467:22-41 | MS.gene61724:intergenic | 55.0% | |
| TGCGTGACAAGGGTGAGGTT+TGG | + | 32467:846-865 | MS.gene61724:CDS | 55.0% | |
| TGGACCGTTATATGGGCGAG+AGG | - | 32467:314-333 | MS.gene61724:intergenic | 55.0% | |
| AGAAGGGAACCAGCCCATGG+GGG | - | 32467:93-112 | MS.gene61724:intergenic | 60.0% | |
| GCCGAAGCCTTGAAGCAACC+TGG | + | 32467:23-42 | MS.gene61724:CDS | 60.0% | |
| GGAACGTCACCCAGAAGAGG+TGG | - | 32467:202-221 | MS.gene61724:intergenic | 60.0% | |
| GGAAGAAGGGAACCAGCCCA+TGG | - | 32467:96-115 | MS.gene61724:intergenic | 60.0% | |
| GGGTTACCGTTTCGGTACCC+AGG | + | 32467:599-618 | MS.gene61724:CDS | 60.0% | |
| CATGGGCCTCCGAGACAACC+AGG | - | 32467:44-63 | MS.gene61724:intergenic | 65.0% | |
| CTGGTTGTCTCGGAGGCCCA+TGG | + | 32467:42-61 | MS.gene61724:CDS | 65.0% | |
| GGAACCAGCCCATGGGGGAA+CGG | - | 32467:88-107 | MS.gene61724:intergenic | 65.0% | |
| TGTGCTCGCCGTTCCCCCAT+GGG | + | 32467:77-96 | MS.gene61724:CDS | 65.0% | |
| TTGTGCTCGCCGTTCCCCCA+TGG | + | 32467:76-95 | MS.gene61724:CDS | 65.0% | |
| CTCGCCGTTCCCCCATGGGC+TGG | + | 32467:81-100 | MS.gene61724:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32467 | gene | 5 | 1090 | 5 | ID=MS.gene61724 |
| 32467 | mRNA | 5 | 1090 | 5 | ID=MS.gene61724.t1;Parent=MS.gene61724 |
| 32467 | exon | 5 | 1090 | 5 | ID=MS.gene61724.t1.exon1;Parent=MS.gene61724.t1 |
| 32467 | CDS | 5 | 1090 | 5 | ID=cds.MS.gene61724.t1;Parent=MS.gene61724.t1 |
| Gene Sequence |
| Protein sequence |
