Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61916.t1 | XP_013467304.1 | 100 | 82 | 0 | 0 | 1 | 82 | 1 | 82 | 5.20E-36 | 159.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61916.t1 | I3S1M4 | 100.0 | 82 | 0 | 0 | 1 | 82 | 1 | 82 | 3.8e-36 | 159.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060702 | MS.gene61916 | 0.810893 | 9.19E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61916.t1 | MTR_1g048960 | 100.000 | 82 | 0 | 0 | 1 | 82 | 1 | 82 | 4.70e-56 | 167 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61916.t1 | AT1G72020 | 69.880 | 83 | 24 | 1 | 1 | 82 | 1 | 83 | 5.33e-37 | 119 |
Find 18 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATCCTCTACTCACTGATT+TGG | 0.327769 | 3.3:-6060547 | None:intergenic |
| CTTGGACAAGATGAAGTATC+AGG | 0.339393 | 3.3:+6060650 | MS.gene61916:CDS |
| AGTAGAGGATTTCATTCAAC+TGG | 0.411924 | 3.3:+6060558 | MS.gene61916:CDS |
| AAGAGGATGGGAGGCGGACA+TGG | 0.484956 | 3.3:+6060585 | MS.gene61916:CDS |
| GTGTTCACAGCTTTCAGCAT+TGG | 0.501579 | 3.3:+6060693 | MS.gene61916:CDS |
| TGCATATCACATGTACAACT+TGG | 0.529028 | 3.3:+6060632 | MS.gene61916:CDS |
| TTCAACTGGTGTGAAGAGGA+TGG | 0.534302 | 3.3:+6060572 | MS.gene61916:CDS |
| TTCATTCAACTGGTGTGAAG+AGG | 0.561102 | 3.3:+6060568 | MS.gene61916:CDS |
| GCATATACTCTGGCTCGCTA+TGG | 0.570951 | 3.3:-6060608 | None:intergenic |
| ACTCACTGATTTGGAGATGG+AGG | 0.581472 | 3.3:-6060538 | None:intergenic |
| TCAACTGGTGTGAAGAGGAT+GGG | 0.584183 | 3.3:+6060573 | MS.gene61916:CDS |
| TCTACTCACTGATTTGGAGA+TGG | 0.595575 | 3.3:-6060541 | None:intergenic |
| GGTGTGAAGAGGATGGGAGG+CGG | 0.610714 | 3.3:+6060579 | MS.gene61916:CDS |
| TTGGACAAGATGAAGTATCA+GGG | 0.612137 | 3.3:+6060651 | MS.gene61916:CDS |
| ATCTCCAAATCAGTGAGTAG+AGG | 0.617769 | 3.3:+6060543 | MS.gene61916:CDS |
| TGATATGCATGCATATACTC+TGG | 0.624731 | 3.3:-6060618 | None:intergenic |
| ATAACGATGGCATTGAACAA+CGG | 0.641834 | 3.3:+6060477 | None:intergenic |
| ACTGGTGTGAAGAGGATGGG+AGG | 0.670600 | 3.3:+6060576 | MS.gene61916:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGTAGAGGATTTCATTCAAC+TGG | + | chr3.3:6060558-6060577 | MS.gene61916:CDS | 35.0% | |
| TGATATGCATGCATATACTC+TGG | - | chr3.3:6060621-6060640 | None:intergenic | 35.0% | |
| TGCATATCACATGTACAACT+TGG | + | chr3.3:6060632-6060651 | MS.gene61916:CDS | 35.0% | |
| ! | AAATCCTCTACTCACTGATT+TGG | - | chr3.3:6060550-6060569 | None:intergenic | 35.0% |
| ! | TTGGACAAGATGAAGTATCA+GGG | + | chr3.3:6060651-6060670 | MS.gene61916:CDS | 35.0% |
| ATCTCCAAATCAGTGAGTAG+AGG | + | chr3.3:6060543-6060562 | MS.gene61916:CDS | 40.0% | |
| TTCATTCAACTGGTGTGAAG+AGG | + | chr3.3:6060568-6060587 | MS.gene61916:CDS | 40.0% | |
| ! | CTTGGACAAGATGAAGTATC+AGG | + | chr3.3:6060650-6060669 | MS.gene61916:CDS | 40.0% |
| !! | TCTACTCACTGATTTGGAGA+TGG | - | chr3.3:6060544-6060563 | None:intergenic | 40.0% |
| GTGTTCACAGCTTTCAGCAT+TGG | + | chr3.3:6060693-6060712 | MS.gene61916:CDS | 45.0% | |
| TCAACTGGTGTGAAGAGGAT+GGG | + | chr3.3:6060573-6060592 | MS.gene61916:CDS | 45.0% | |
| TTCAACTGGTGTGAAGAGGA+TGG | + | chr3.3:6060572-6060591 | MS.gene61916:CDS | 45.0% | |
| !! | ACTCACTGATTTGGAGATGG+AGG | - | chr3.3:6060541-6060560 | None:intergenic | 45.0% |
| ! | GCATATACTCTGGCTCGCTA+TGG | - | chr3.3:6060611-6060630 | None:intergenic | 50.0% |
| ACTGGTGTGAAGAGGATGGG+AGG | + | chr3.3:6060576-6060595 | MS.gene61916:CDS | 55.0% | |
| AAGAGGATGGGAGGCGGACA+TGG | + | chr3.3:6060585-6060604 | MS.gene61916:CDS | 60.0% | |
| GGTGTGAAGAGGATGGGAGG+CGG | + | chr3.3:6060579-6060598 | MS.gene61916:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 6060483 | 6060730 | 6060483 | ID=MS.gene61916 |
| chr3.3 | mRNA | 6060483 | 6060730 | 6060483 | ID=MS.gene61916.t1;Parent=MS.gene61916 |
| chr3.3 | exon | 6060483 | 6060730 | 6060483 | ID=MS.gene61916.t1.exon1;Parent=MS.gene61916.t1 |
| chr3.3 | CDS | 6060483 | 6060730 | 6060483 | ID=cds.MS.gene61916.t1;Parent=MS.gene61916.t1 |
| Gene Sequence |
| Protein sequence |
