AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62523

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62523.t1 MTR_7g029525 94.125 817 48 0 1 817 508 1324 0.0 1566
MS.gene62523.t1 MTR_7g450790 83.272 819 127 4 4 817 626 1439 0.0 1363
MS.gene62523.t1 MTR_7g450790 65.278 72 25 0 61 132 461 532 2.00e-20 97.8
MS.gene62523.t1 MTR_4g088650 79.659 821 133 4 2 817 427 1218 0.0 1288
MS.gene62523.t1 MTR_7g450780 67.571 848 231 14 2 817 511 1346 0.0 1065
MS.gene62523.t1 MTR_7g450780 64.384 73 26 0 61 133 387 459 2.34e-19 94.4
MS.gene62523.t1 MTR_7g450820 67.028 831 246 10 2 816 416 1234 0.0 1063
MS.gene62523.t1 MTR_7g050445 66.187 834 248 13 2 817 481 1298 0.0 1060
MS.gene62523.t1 MTR_7g450860 65.306 833 255 13 4 816 454 1272 0.0 1020
MS.gene62523.t1 MTR_2g084695 67.514 354 43 2 464 817 9 290 8.54e-151 443
MS.gene62523.t1 MTR_1g081750 32.986 767 440 18 76 798 363 1099 2.71e-117 382
MS.gene62523.t1 MTR_7g026650 31.865 772 463 17 82 817 408 1152 1.89e-110 365
MS.gene62523.t1 MTR_3g071860 32.922 729 431 17 111 798 539 1250 2.35e-108 360
MS.gene62523.t1 MTR_5g083300 30.702 798 493 17 43 798 508 1287 1.49e-104 350
MS.gene62523.t1 MTR_5g083300 30.702 798 493 17 43 798 545 1324 2.44e-104 350
MS.gene62523.t1 MTR_8g037345 29.063 726 427 18 111 810 234 897 4.30e-87 296
MS.gene62523.t1 MTR_2g437480 31.378 682 376 18 111 767 387 1001 9.23e-87 297
MS.gene62523.t1 MTR_7g026620 25.997 577 298 16 155 713 177 642 1.13e-45 174
MS.gene62523.t1 MTR_5g004720 24.493 592 351 15 216 771 161 692 1.05e-35 146
MS.gene62523.t1 MTR_8g094070 25.043 583 331 20 231 770 373 892 1.78e-28 123
MS.gene62523.t1 MTR_8g094070 25.043 583 331 20 231 770 373 892 1.83e-28 123
MS.gene62523.t1 MTR_1g069755 24.490 588 327 21 222 767 371 883 3.39e-27 119
MS.gene62523.t1 MTR_3g106210 23.921 556 343 13 278 802 309 815 5.23e-25 112
MS.gene62523.t1 MTR_3g106210 23.921 556 343 13 278 802 309 815 5.75e-25 112
MS.gene62523.t1 MTR_3g106210 23.921 556 343 13 278 802 309 815 6.34e-25 112
MS.gene62523.t1 MTR_3g106210 23.921 556 343 13 278 802 309 815 6.35e-25 112
MS.gene62523.t1 MTR_1g013190 23.211 629 392 19 205 794 228 804 5.03e-24 109
MS.gene62523.t1 MTR_1g035330 25.520 529 304 20 276 753 215 704 1.83e-23 106
MS.gene62523.t1 MTR_5g020000 23.656 558 327 17 279 806 206 694 6.16e-23 105
MS.gene62523.t1 MTR_4g096930 25.862 348 207 11 419 750 870 1182 3.39e-22 103
MS.gene62523.t1 MTR_1g080420 37.267 161 93 2 614 766 984 1144 6.76e-22 102
MS.gene62523.t1 MTR_1g080420 37.267 161 93 2 614 766 1115 1275 7.04e-22 102
MS.gene62523.t1 MTR_2g020000 24.366 513 294 15 279 774 213 648 2.29e-19 94.0
MS.gene62523.t1 MTR_4g118845 24.863 366 233 9 420 775 126 459 2.44e-19 94.0
MS.gene62523.t1 MTR_3g053910 22.686 551 325 16 243 772 571 1041 3.61e-19 94.0
MS.gene62523.t1 MTR_3g053910 22.686 551 325 16 243 772 600 1070 3.91e-19 93.6
MS.gene62523.t1 MTR_4g118845 25.067 371 224 10 420 774 313 645 4.94e-19 93.2
MS.gene62523.t1 MTR_5g005840 23.780 492 283 17 279 750 385 804 7.02e-19 92.4
MS.gene62523.t1 MTR_5g090090 21.480 554 328 18 243 772 617 1087 1.17e-18 92.0
MS.gene62523.t1 MTR_5g090090 21.480 554 328 18 243 772 617 1087 1.30e-18 92.0
MS.gene62523.t1 MTR_4g118720 25.000 360 208 10 420 751 1132 1457 5.06e-18 90.1
MS.gene62523.t1 MTR_1g105050 25.000 500 268 19 279 750 398 818 7.87e-18 89.4
MS.gene62523.t1 MTR_4g035100 26.131 398 240 14 421 773 1581 1969 9.30e-18 89.4
MS.gene62523.t1 MTR_4g035100 26.131 398 240 14 421 773 1581 1969 9.71e-18 89.4
MS.gene62523.t1 MTR_4g078495 36.170 141 84 2 630 770 538 672 6.98e-17 85.9
MS.gene62523.t1 MTR_4g078460 36.170 141 84 2 630 770 824 958 7.44e-17 86.3
MS.gene62523.t1 MTR_1g044120 35.897 156 93 3 608 762 981 1130 2.77e-16 84.3
MS.gene62523.t1 MTR_1g053955 31.481 162 105 2 606 767 1055 1210 8.68e-16 82.8
MS.gene62523.t1 MTR_7g045505 24.485 388 242 12 421 781 126 489 1.71e-15 81.3
MS.gene62523.t1 MTR_7g045505 24.485 388 242 12 421 781 126 489 2.48e-15 80.9
MS.gene62523.t1 MTR_7g089190 37.879 132 76 2 631 761 668 794 5.62e-15 79.7
MS.gene62523.t1 MTR_1g115215 30.769 143 93 2 631 773 887 1023 9.68e-14 75.9
MS.gene62523.t1 MTR_2g084630 34.559 136 84 2 631 762 868 1002 2.41e-12 71.2
MS.gene62523.t1 MTR_2g012830 31.081 148 94 3 627 773 1146 1286 2.48e-12 71.6
MS.gene62523.t1 MTR_4g049500 32.571 175 102 6 595 767 750 910 1.65e-11 68.6
MS.gene62523.t1 MTR_2g084630 32.836 134 80 3 631 762 874 999 3.19e-11 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62523.t1 AT1G05490 48.638 771 324 18 85 817 666 1402 0.0 655
MS.gene62523.t1 AT3G24340 46.100 718 342 13 106 816 448 1127 0.0 587
MS.gene62523.t1 AT3G42670 33.655 725 423 18 111 801 541 1241 7.83e-109 362
MS.gene62523.t1 AT3G42670 33.655 725 423 18 111 801 541 1241 7.83e-109 362
MS.gene62523.t1 AT3G42670 33.655 725 423 18 111 801 541 1241 7.83e-109 362
MS.gene62523.t1 AT3G42670 33.425 727 424 18 111 801 541 1243 2.82e-108 360
MS.gene62523.t1 AT5G20420 33.381 695 414 15 111 774 547 1223 2.18e-106 355
MS.gene62523.t1 AT2G16390 31.646 632 359 16 155 767 270 847 5.09e-88 298
MS.gene62523.t1 AT2G16390 31.646 632 359 16 155 767 273 850 5.37e-88 298
MS.gene62523.t1 AT2G21450 31.232 698 388 23 110 777 57 692 2.52e-87 292
MS.gene62523.t1 AT2G21450 31.541 688 379 23 110 767 148 773 8.79e-87 293
MS.gene62523.t1 AT2G21450 31.541 688 379 23 110 767 155 780 1.91e-86 292
MS.gene62523.t1 AT3G19210 24.694 571 332 16 239 770 176 687 2.31e-33 138
MS.gene62523.t1 AT3G19210 24.694 571 332 16 239 770 176 687 3.45e-33 138
MS.gene62523.t1 AT3G19210 24.253 569 335 17 239 770 176 685 1.32e-32 136
MS.gene62523.t1 AT2G18760 26.110 563 306 21 243 767 444 934 8.11e-29 124
MS.gene62523.t1 AT2G18760 26.110 563 306 21 243 767 385 875 8.68e-29 124
MS.gene62523.t1 AT2G18760 26.110 563 306 21 243 767 385 875 8.68e-29 124
MS.gene62523.t1 AT2G18760 26.110 563 306 21 243 767 385 875 8.68e-29 124
MS.gene62523.t1 AT2G18760 26.110 563 306 21 243 767 385 875 8.68e-29 124
MS.gene62523.t1 AT2G18760 26.110 563 306 21 243 767 385 875 8.68e-29 124
MS.gene62523.t1 AT2G18760 26.110 563 306 21 243 767 385 875 8.68e-29 124
MS.gene62523.t1 AT4G31900 22.924 602 357 19 218 794 199 718 4.33e-27 119
MS.gene62523.t1 AT4G31900 22.924 602 357 19 218 794 158 677 4.61e-27 119
MS.gene62523.t1 AT4G31900 22.924 602 357 19 218 794 199 718 6.36e-27 118
MS.gene62523.t1 AT5G63950 24.248 565 317 20 243 762 377 875 3.32e-25 113
MS.gene62523.t1 AT5G63950 25.166 453 252 15 368 762 435 858 2.29e-23 107
MS.gene62523.t1 AT5G44800 23.768 568 332 22 206 750 648 1137 3.75e-23 107
MS.gene62523.t1 AT2G25170 22.667 600 371 17 233 801 288 825 9.36e-23 105
MS.gene62523.t1 AT2G25170 22.667 600 371 17 233 801 262 799 1.03e-22 105
MS.gene62523.t1 AT2G25170 22.667 600 371 17 233 801 262 799 1.03e-22 105
MS.gene62523.t1 AT2G25170 22.667 600 371 17 233 801 279 816 1.13e-22 105
MS.gene62523.t1 AT1G08600 36.025 161 95 2 614 766 1116 1276 6.33e-21 99.4
MS.gene62523.t1 AT1G08600 36.025 161 95 2 614 766 1116 1276 6.33e-21 99.4
MS.gene62523.t1 AT1G08600 36.025 161 95 2 614 766 1116 1276 6.33e-21 99.4
MS.gene62523.t1 AT1G08600 36.025 161 95 2 614 766 1095 1255 7.40e-21 99.4
MS.gene62523.t1 AT2G13370 23.423 555 316 21 242 772 624 1093 1.74e-20 98.2
MS.gene62523.t1 AT2G13370 23.423 555 316 21 242 772 624 1093 1.74e-20 98.2
MS.gene62523.t1 AT2G13370 23.423 555 316 21 242 772 624 1093 1.74e-20 98.2
MS.gene62523.t1 AT5G66750 23.508 553 334 18 279 807 224 711 7.02e-20 95.5
MS.gene62523.t1 AT5G18620 23.197 513 300 14 279 774 216 651 3.06e-18 90.5
MS.gene62523.t1 AT5G18620 24.384 365 234 8 420 774 319 651 3.06e-18 90.5
MS.gene62523.t1 AT3G06400 23.720 371 229 8 420 774 314 646 6.85e-18 89.4
MS.gene62523.t1 AT3G06400 23.720 371 229 8 420 774 314 646 7.09e-18 89.4
MS.gene62523.t1 AT3G06400 23.720 371 229 8 420 774 314 646 7.22e-18 89.4
MS.gene62523.t1 AT5G19310 23.517 489 288 15 279 750 144 563 7.59e-18 89.0
MS.gene62523.t1 AT2G28290 24.863 366 201 10 420 751 881 1206 8.34e-18 89.7
MS.gene62523.t1 AT2G28290 24.863 366 201 10 420 751 882 1207 8.34e-18 89.7
MS.gene62523.t1 AT2G28290 24.863 366 201 10 420 751 881 1206 8.61e-18 89.7
MS.gene62523.t1 AT2G28290 24.863 366 201 10 420 751 881 1206 8.76e-18 89.7
MS.gene62523.t1 AT2G28290 24.863 366 201 10 420 751 881 1206 8.76e-18 89.7
MS.gene62523.t1 AT2G28290 24.863 366 201 10 420 751 882 1207 8.91e-18 89.4
MS.gene62523.t1 AT5G19310 23.517 489 288 15 279 750 408 827 1.10e-17 89.0
MS.gene62523.t1 AT1G03750 36.170 141 84 2 630 770 545 679 2.80e-17 87.4
MS.gene62523.t1 AT2G44980 25.840 387 217 13 421 777 181 527 4.80e-17 86.7
MS.gene62523.t1 AT5G43530 39.850 133 74 2 630 762 1128 1254 7.71e-17 86.3
MS.gene62523.t1 AT3G06010 24.432 352 211 11 420 750 528 845 1.25e-16 85.5
MS.gene62523.t1 AT2G44980 26.396 394 219 15 421 777 186 545 1.36e-16 85.1
MS.gene62523.t1 AT2G44980 26.396 394 219 15 421 777 187 546 1.45e-16 85.1
MS.gene62523.t1 AT3G12810 32.099 162 104 2 606 767 1064 1219 2.84e-16 84.3
MS.gene62523.t1 AT3G12810 32.099 162 104 2 606 767 1068 1223 3.09e-16 84.3
MS.gene62523.t1 AT5G05130 36.025 161 91 3 631 790 727 876 3.19e-16 84.0
MS.gene62523.t1 AT3G32330 23.018 391 199 12 237 605 17 327 3.19e-14 75.1
MS.gene62523.t1 AT1G11100 30.573 157 103 2 630 786 929 1079 5.58e-14 76.6
MS.gene62523.t1 AT1G11100 30.573 157 103 2 630 786 973 1123 6.39e-14 76.6
MS.gene62523.t1 AT1G11100 30.573 157 103 2 630 786 1072 1222 7.36e-14 76.6
MS.gene62523.t1 AT1G11100 30.573 157 103 2 630 786 1116 1266 7.90e-14 76.3
MS.gene62523.t1 AT1G11100 30.573 157 103 2 630 786 1115 1265 8.25e-14 76.3
MS.gene62523.t1 AT3G54280 31.973 147 93 3 629 773 1830 1971 2.70e-13 74.7
MS.gene62523.t1 AT3G54280 31.973 147 93 3 629 773 1861 2002 2.99e-13 74.7
MS.gene62523.t1 AT1G61140 31.915 141 88 3 630 769 966 1099 2.09e-12 71.6
MS.gene62523.t1 AT1G61140 31.915 141 88 3 630 769 1124 1257 2.10e-12 71.6
MS.gene62523.t1 AT3G54460 35.714 140 78 5 627 762 894 1025 2.80e-12 71.2
MS.gene62523.t1 AT3G54460 35.714 140 78 5 627 762 1191 1322 3.21e-12 71.2
MS.gene62523.t1 AT1G50410 30.137 146 90 3 631 773 828 964 4.17e-12 70.5
MS.gene62523.t1 AT1G50410 30.137 146 90 3 631 773 828 964 4.17e-12 70.5
MS.gene62523.t1 AT1G50410 30.137 146 90 3 631 773 828 964 4.17e-12 70.5
MS.gene62523.t1 AT3G20010 30.769 143 93 2 631 773 628 764 6.51e-12 69.7
MS.gene62523.t1 AT3G20010 30.769 143 93 2 631 773 603 739 6.55e-12 69.7
MS.gene62523.t1 AT3G20010 30.769 143 93 2 631 773 894 1030 1.16e-11 69.3
MS.gene62523.t1 AT3G20010 30.769 143 93 2 631 773 894 1030 1.16e-11 69.3
MS.gene62523.t1 AT3G20010 30.769 143 93 2 631 773 894 1030 1.16e-11 69.3
MS.gene62523.t1 AT5G22750 30.968 155 101 2 608 762 858 1006 1.99e-11 68.6
MS.gene62523.t1 AT1G02670 32.639 144 89 3 631 773 539 675 3.66e-11 67.4
MS.gene62523.t1 AT1G02670 32.639 144 89 3 631 773 512 648 3.91e-11 67.0
MS.gene62523.t1 AT1G02670 32.639 144 89 3 631 773 516 652 5.88e-11 66.6
MS.gene62523.t1 AT1G02670 32.639 144 89 3 631 773 527 663 6.10e-11 66.6
MS.gene62523.t1 AT1G05120 34.783 138 78 4 631 767 725 851 6.27e-11 66.6
MS.gene62523.t1 AT1G05120 34.783 138 78 4 631 767 682 808 7.33e-11 66.6

Find 171 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTACAGATTGGAAATTT+AGG 0.126449 7.3:-70287114 MS.gene62523:intron
ATATGACTTCTTTGATTTCT+TGG 0.141776 7.3:+70285907 None:intergenic
CTCCATTCCAGAACAATTTC+TGG 0.174401 7.3:-70285831 MS.gene62523:CDS
TATGGTCAGCCTTGTCTTTC+CGG 0.181588 7.3:+70286332 None:intergenic
AGCTTCTAGGGTTGTACTTC+TGG 0.196779 7.3:-70285074 MS.gene62523:CDS
GAAATCAAAGAAGTCATATT+TGG 0.244946 7.3:-70285903 MS.gene62523:CDS
ATTCCTTCTTCTTCTCAATA+TGG 0.245100 7.3:+70287605 None:intergenic
CGGAGTTGTGAACCTGTTTC+GGG 0.246918 7.3:-70285703 MS.gene62523:CDS
TTCAAAGAAACAAGCATTCT+TGG 0.249693 7.3:-70286069 MS.gene62523:CDS
AGCCTTGTCTTTCCGGTTCC+AGG 0.258151 7.3:+70286339 None:intergenic
AACAATAATCGGAAAGCATT+TGG 0.260267 7.3:+70286278 None:intergenic
ATGATTTATGTCCCTGCTTA+TGG 0.263420 7.3:+70285734 None:intergenic
TATAAAGTTTGTTTCAAATC+TGG 0.265369 7.3:+70286480 None:intergenic
GCGGAGTTGTGAACCTGTTT+CGG 0.280802 7.3:-70285704 MS.gene62523:CDS
ATTTAAAAGCAGAGGTTAAT+TGG 0.281211 7.3:+70285248 None:intergenic
AAGAAGGCTTTGAATTCATT+TGG 0.281224 7.3:-70286446 MS.gene62523:CDS
TGCTCCTTGCGCAATGCTAT+TGG 0.289113 7.3:-70287522 MS.gene62523:CDS
ATCTACAGATTGGAAATTTA+GGG 0.298187 7.3:-70287113 MS.gene62523:intron
AAAATAAGCTTCCTGGGTTA+AGG 0.301129 7.3:-70285588 MS.gene62523:CDS
TTAGCTTAGTTGGAGCTTCT+AGG 0.302332 7.3:-70285087 MS.gene62523:CDS
TTTGTTTCAAATCTGGAATT+AGG 0.316184 7.3:+70286487 None:intergenic
CGAAGTGTTATGGGATGAAT+TGG 0.316690 7.3:-70287332 MS.gene62523:CDS
AGGAGGAGAACGAAGTGTTA+TGG 0.324265 7.3:-70287342 MS.gene62523:CDS
CTTGTTGTGTTGCCCGAAAC+AGG 0.324806 7.3:+70285691 None:intergenic
TTAAGCAATCCCGAGTTATC+TGG 0.334660 7.3:-70286183 MS.gene62523:CDS
CAGAAGCGAATCATCCTTTC+CGG 0.338463 7.3:-70285856 MS.gene62523:CDS
GGAATCAGCTACAGTTTGTT+TGG 0.342280 7.3:-70286048 MS.gene62523:CDS
ATCCTTGAAAATAAGCTTCC+TGG 0.344675 7.3:-70285595 MS.gene62523:CDS
TCCTGGGTTAAGGGACTGTT+TGG 0.348954 7.3:-70285578 MS.gene62523:CDS
CACAGTTGAATCATTCTTCA+TGG 0.355596 7.3:+70287486 None:intergenic
GGAGGAGAACGAAGTGTTAT+GGG 0.356347 7.3:-70287341 MS.gene62523:CDS
CTTGCAATGAAGGCACAGTT+TGG 0.359027 7.3:-70286512 MS.gene62523:CDS
ATTGTAAAGGCGCATGGAAT+TGG 0.364774 7.3:+70285281 None:intergenic
ACTAATTTCTCTTGCAATGA+AGG 0.366544 7.3:-70286522 MS.gene62523:CDS
AAATAAGCTTCCTGGGTTAA+GGG 0.369051 7.3:-70285587 MS.gene62523:CDS
TCTGAGGGAATTAGCTTAGT+TGG 0.375316 7.3:-70285097 MS.gene62523:CDS
GGGAGGATGAATTCAAGAAA+TGG 0.377896 7.3:-70286230 MS.gene62523:CDS
AGATTTCCCTGTGAAGGTTC+AGG 0.380692 7.3:-70286624 MS.gene62523:CDS
CTGCTAGTATACTACTAACT+TGG 0.381021 7.3:-70286251 MS.gene62523:CDS
TCTGCTCGCTGAAGGGACTA+TGG 0.381344 7.3:-70284966 MS.gene62523:CDS
CAAGAAGCTGAAATTGCTAA+TGG 0.382622 7.3:-70285647 MS.gene62523:CDS
GCTCCTTGCGCAATGCTATT+GGG 0.383553 7.3:-70287521 MS.gene62523:CDS
CTTTCTTCCTGAACCTTCAC+AGG 0.384915 7.3:+70286617 None:intergenic
AGCTCAGGCGGTATCGTGTT+AGG 0.387985 7.3:+70285769 None:intergenic
ATTACGGATTGCTTGACCTT+TGG 0.391840 7.3:+70285181 None:intergenic
ATTTCTCATGCTCCTGGAAC+CGG 0.395216 7.3:-70286351 MS.gene62523:CDS
CTTGGACTCATCGTCATTAT+CGG 0.401265 7.3:+70284871 None:intergenic
ACGGATTGCTTGACCTTTGG+AGG 0.405791 7.3:+70285184 None:intergenic
AAAGCTCCCAGAAATTGTTC+TGG 0.406710 7.3:+70285824 None:intergenic
GGAAGAAAGGCATCATTTGA+TGG 0.407266 7.3:-70286603 MS.gene62523:CDS
CTAGCAGGAGCAACAATAAT+CGG 0.410720 7.3:+70286267 None:intergenic
ACAATAATCGGAAAGCATTT+GGG 0.412501 7.3:+70286279 None:intergenic
AAAATTCATATCACAGCTCT+TGG 0.412571 7.3:+70284853 None:intergenic
ATTGTTCTGGAATGGAGTTC+CGG 0.412671 7.3:+70285837 None:intergenic
TTTCCTTGAATGTTCTCTTT+CGG 0.413344 7.3:-70285440 MS.gene62523:CDS
CTTCCGAAAGAGAACATTCA+AGG 0.414255 7.3:+70285437 None:intergenic
TTCAACTGTGTTCGAGTTCA+AGG 0.414802 7.3:-70287473 MS.gene62523:CDS
ATCGTTGAAGCTATGGATTA+CGG 0.422848 7.3:+70285165 None:intergenic
ACTGAGGAAGGAGCGAGATA+TGG 0.423086 7.3:-70284762 MS.gene62523:CDS
TGCTCTCGACAAGGATCAGC+TGG 0.426412 7.3:-70285404 MS.gene62523:CDS
TGCATTATTTCTCATGCTCC+TGG 0.431774 7.3:-70286357 MS.gene62523:CDS
CCTTTGTTCATGTGCACAAA+GGG 0.432796 7.3:-70285621 MS.gene62523:CDS
AACAAAGGAAGGGATTATAA+AGG 0.438543 7.3:-70287640 MS.gene62523:CDS
TTGTACTTCTGGATGTTGTA+TGG 0.448145 7.3:-70285063 MS.gene62523:CDS
ATATGGTAAACAGGCTGAAA+AGG 0.451018 7.3:-70284933 MS.gene62523:CDS
TGGTCTTTAATTAATTGTAA+AGG 0.452462 7.3:+70285268 None:intergenic
AAGGAAGTGTTGTTCATGTC+TGG 0.458301 7.3:-70285214 MS.gene62523:CDS
TCCATTCCAGAACAATTTCT+GGG 0.462331 7.3:-70285830 MS.gene62523:CDS
AAGCTATCAGCCGAGCGTAT+AGG 0.462639 7.3:-70285021 MS.gene62523:CDS
TCGTGTTCACATCGCGATGA+TGG 0.467848 7.3:+70287057 None:intergenic
TTGCAATGAAGGCACAGTTT+GGG 0.468703 7.3:-70286511 MS.gene62523:CDS
GTAAATGTTTCACTGTTTGA+TGG 0.471326 7.3:-70286579 MS.gene62523:CDS
TAGCTTAGTTGGAGCTTCTA+GGG 0.471979 7.3:-70285086 MS.gene62523:CDS
TTCTTAAGGTTCCATATCGA+TGG 0.475228 7.3:+70287394 None:intergenic
GAAAATGTGAAGGGGAGAAA+AGG 0.475800 7.3:-70285997 MS.gene62523:CDS
GCAAAAGACCATGGAGGAGG+AGG 0.480692 7.3:-70287359 MS.gene62523:CDS
ATAGCTTGTCTTTCGACTGA+AGG 0.494216 7.3:+70285037 None:intergenic
GGAGGATGAATTCAAGAAAT+GGG 0.494983 7.3:-70286229 MS.gene62523:CDS
AGAACGCTTCACTAAGCTTA+AGG 0.496015 7.3:+70285795 None:intergenic
CTGTGAAGGTTCAGGAAGAA+AGG 0.497585 7.3:-70286616 MS.gene62523:CDS
CAAGTTAGTAGTATACTAGC+AGG 0.498458 7.3:+70286252 None:intergenic
TTCGTTCTCCTCCTCCTCCA+TGG 0.498689 7.3:+70287351 None:intergenic
GGTTTGTTAGTTCTAGATGA+AGG 0.499262 7.3:-70285937 MS.gene62523:CDS
AATTTGCATCGTTGAAGCTA+TGG 0.500809 7.3:+70285158 None:intergenic
CTCAGACCATTTAAAAGCAG+AGG 0.505543 7.3:+70285240 None:intergenic
TCCTTGAAAATAAGCTTCCT+GGG 0.507367 7.3:-70285594 MS.gene62523:CDS
TCTTAAGGTTCCATATCGAT+GGG 0.513042 7.3:+70287395 None:intergenic
GGATTGCTTAAATTGTGAAA+CGG 0.514265 7.3:+70286195 None:intergenic
GGAATCAAGTAAACAGAGAA+TGG 0.514605 7.3:-70287668 MS.gene62523:CDS
ATATGCCTTGAGAAACACTA+TGG 0.514676 7.3:+70286314 None:intergenic
TTCTTGATCTTATCATCAGA+AGG 0.516557 7.3:+70285664 None:intergenic
CCTTTGTGCACATGAACAAA+GGG 0.519201 7.3:+70285622 None:intergenic
GCCACCTTCACTGCAAGGAT+CGG 0.519684 7.3:+70286380 None:intergenic
CATCCATATTGAGAAGAAGA+AGG 0.520459 7.3:-70287608 MS.gene62523:CDS
AAGATTCTGCTAAAAGCTCC+TGG 0.520895 7.3:-70285958 MS.gene62523:CDS
CGCTGAAGGGACTATGGAGG+AGG 0.528705 7.3:-70284960 MS.gene62523:CDS
GGACTGTTTGGTGACTTTGA+AGG 0.530361 7.3:-70285566 MS.gene62523:CDS
GTTGATAGATTTCCCTGTGA+AGG 0.531771 7.3:-70286630 MS.gene62523:CDS
TAATTAATTGTAAAGGCGCA+TGG 0.533525 7.3:+70285275 None:intergenic
CCCTTTGTTCATGTGCACAA+AGG 0.533803 7.3:-70285622 MS.gene62523:CDS
GTCTCCACAGAAACCGGTGG+TGG 0.533923 7.3:+70287421 None:intergenic
CCCTTTGTGCACATGAACAA+AGG 0.536847 7.3:+70285621 None:intergenic
CAAATATATCTCACCACTAG+TGG 0.541968 7.3:-70286971 MS.gene62523:intron
ACAGCCACCACCGGTTTCTG+TGG 0.546294 7.3:-70287425 MS.gene62523:CDS
ATCAGCCGAGCGTATAGGAT+TGG 0.547339 7.3:-70285016 MS.gene62523:CDS
GAATGGTGGAGAACAAAGGA+AGG 0.552165 7.3:-70287651 MS.gene62523:CDS
ATGCGAATAGAACCTTTGCT+TGG 0.557067 7.3:+70285134 None:intergenic
CTTAGACTGAACCCATATGA+AGG 0.561386 7.3:-70285376 MS.gene62523:CDS
AATGCCGATCCTTGCAGTGA+AGG 0.561759 7.3:-70286384 MS.gene62523:CDS
TAGTCCCTTCAGCGAGCAGA+TGG 0.563533 7.3:+70284969 None:intergenic
ATGTTCTTTACCAGATAACT+CGG 0.563939 7.3:+70286173 None:intergenic
TCCCAGAAATTGTTCTGGAA+TGG 0.564172 7.3:+70285829 None:intergenic
TTATATCGTCATCAACAAGA+AGG 0.567121 7.3:-70286462 MS.gene62523:CDS
TCTGACCAATCCTATACGCT+CGG 0.568050 7.3:+70285011 None:intergenic
ATTCAAGGAAAAGCGATGGA+TGG 0.568688 7.3:+70285452 None:intergenic
CATACCATCTGCTCGCTGAA+GGG 0.572458 7.3:-70284973 MS.gene62523:CDS
TCTACAAAAGCTTGTTCTGA+GGG 0.573558 7.3:-70285112 MS.gene62523:CDS
AGAATCTGACGCTGTATCCA+TGG 0.574662 7.3:-70287296 MS.gene62523:intron
AAGGAAAAGCGATGGATGGA+CGG 0.575965 7.3:+70285456 None:intergenic
GAACATTCAAGGAAAAGCGA+TGG 0.581694 7.3:+70285448 None:intergenic
AATCGGAAAGCATTTGGGAA+AGG 0.582011 7.3:+70286284 None:intergenic
CGATGCAAATTGCCAAGCAA+AGG 0.584100 7.3:-70285146 MS.gene62523:CDS
ATCGATATGGAACCTTAAGA+AGG 0.586361 7.3:-70287392 MS.gene62523:CDS
AAGGAATCTATTGATAACAA+TGG 0.588038 7.3:-70287589 MS.gene62523:CDS
TCAAAGAAGTCATATTTGGA+AGG 0.589347 7.3:-70285899 MS.gene62523:CDS
ACATACCATCTGCTCGCTGA+AGG 0.589622 7.3:-70284974 MS.gene62523:CDS
TGCTAGTATACTACTAACTT+GGG 0.589951 7.3:-70286250 MS.gene62523:CDS
ATGCAGCCACCTTCACTGCA+AGG 0.591972 7.3:+70286375 None:intergenic
TGTTTGGTGACTTTGAAGGC+AGG 0.596768 7.3:-70285562 MS.gene62523:CDS
TAAGTGCGTGGTACTGAGGA+AGG 0.597578 7.3:-70284774 MS.gene62523:CDS
ATCTACAAAAGCTTGTTCTG+AGG 0.600049 7.3:-70285113 MS.gene62523:CDS
GTAAACAGGCTGAAAAGGAC+CGG 0.601881 7.3:-70284928 MS.gene62523:CDS
TCTTGATCTTATCATCAGAA+GGG 0.604374 7.3:+70285665 None:intergenic
AATGGTGGAGAACAAAGGAA+GGG 0.609814 7.3:-70287650 MS.gene62523:CDS
TTTCTTCCTGAACCTTCACA+GGG 0.609885 7.3:+70286618 None:intergenic
TGTTCTTTACCAGATAACTC+GGG 0.610730 7.3:+70286174 None:intergenic
CAGAGAATGGTGGAGAACAA+AGG 0.613980 7.3:-70287655 MS.gene62523:CDS
GATATGGAACCTTAAGAAGG+TGG 0.615242 7.3:-70287389 MS.gene62523:CDS
TCTGGAATGGAGTTCCGGAA+AGG 0.615617 7.3:+70285842 None:intergenic
ACAAGCTACACAGCCACCAC+CGG 0.619069 7.3:-70287434 MS.gene62523:CDS
GCCGATCCTTGCAGTGAAGG+TGG 0.620537 7.3:-70286381 MS.gene62523:CDS
GTGTTCGAGTTCAAGGAGGA+TGG 0.621068 7.3:-70287466 MS.gene62523:CDS
GGTGTATATTGCAAATTGTG+TGG 0.625204 7.3:-70287012 MS.gene62523:CDS
CTTAAGGTTCCATATCGATG+GGG 0.625433 7.3:+70287396 None:intergenic
AGTGCAAAAGACCATGGAGG+AGG 0.626765 7.3:-70287362 MS.gene62523:CDS
AGACATGTTTGTTAAGTGCG+TGG 0.627760 7.3:-70284786 MS.gene62523:CDS
ATCAAGTAAACAGAGAATGG+TGG 0.632548 7.3:-70287665 MS.gene62523:CDS
TGTCCCAATAGCATTGCGCA+AGG 0.633073 7.3:+70287518 None:intergenic
AAAAGCATGAAAATGTGAAG+GGG 0.633486 7.3:-70286005 MS.gene62523:CDS
GCTGACCATAGTGTTTCTCA+AGG 0.634254 7.3:-70286319 MS.gene62523:CDS
TTAAGGTTCCATATCGATGG+GGG 0.634311 7.3:+70287397 None:intergenic
AGAAGAATCTGCTCTCGACA+AGG 0.638283 7.3:-70285413 MS.gene62523:CDS
GAAACCGTGCTTACCACTAG+TGG 0.644506 7.3:+70286958 None:intergenic
ATATTGCAAATTGTGTGGTG+TGG 0.649175 7.3:-70287007 MS.gene62523:CDS
GAATTCAAGAAATGGGACAT+CGG 0.650153 7.3:-70286222 MS.gene62523:CDS
CTCCTGGAACCGGAAAGACA+AGG 0.656255 7.3:-70286341 MS.gene62523:CDS
TCATCTAGAACTAACAAACC+AGG 0.659449 7.3:+70285940 None:intergenic
GTCTGGCAAAGATCCTCCAA+AGG 0.659751 7.3:-70285197 MS.gene62523:CDS
CTTTGTTCATGTGCACAAAG+GGG 0.661128 7.3:-70285620 MS.gene62523:CDS
AACAAAGCAAGAAATGATGG+AGG 0.661151 7.3:-70287689 None:intergenic
ACCAAACAGTCCCTTAACCC+AGG 0.663907 7.3:+70285577 None:intergenic
TAGTATACTACTAACTTGGG+AGG 0.670120 7.3:-70286247 MS.gene62523:CDS
GCTCGCTGAAGGGACTATGG+AGG 0.670171 7.3:-70284963 MS.gene62523:CDS
GGTGTCTCCACAGAAACCGG+TGG 0.680014 7.3:+70287418 None:intergenic
TAGGATTGGTCAGAAGAGAG+TGG 0.680264 7.3:-70285002 MS.gene62523:CDS
AACTGTGTTCGAGTTCAAGG+AGG 0.688944 7.3:-70287470 MS.gene62523:CDS
TTGTTAAGTGCGTGGTACTG+AGG 0.691138 7.3:-70284778 MS.gene62523:CDS
GGACTCATCGTCATTATCGG+CGG 0.691472 7.3:+70284874 None:intergenic
TGGTAAAGAACATGCTGATG+CGG 0.695876 7.3:-70286163 MS.gene62523:CDS
AGCATAATATTAATGAAACG+CGG 0.743459 7.3:-70286113 MS.gene62523:CDS
AAAAGTGCAAAAGACCATGG+AGG 0.766977 7.3:-70287365 MS.gene62523:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGATTTATGTAAAATAACT+TGG - chr7.3:70285501-70285520 MS.gene62523:CDS 15.0%
!!! ATTTTAATTTTGTGAACTAT+AGG - chr7.3:70285199-70285218 MS.gene62523:CDS 15.0%
!! CTGTTTATATGTTTAAACTT+CGG - chr7.3:70285258-70285277 MS.gene62523:CDS 20.0%
!! GAAGCTACAAAAATAAAAAA+CGG - chr7.3:70285349-70285368 MS.gene62523:CDS 20.0%
!! TGGTCTTTAATTAATTGTAA+AGG + chr7.3:70287168-70287187 None:intergenic 20.0%
!! TTATCAACATATATATCACA+AGG + chr7.3:70285741-70285760 None:intergenic 20.0%
!!! AAACTTAAATGGTCTTAAAA+TGG + chr7.3:70285170-70285189 None:intergenic 20.0%
!!! TATAAAGTTTGTTTCAAATC+TGG + chr7.3:70285956-70285975 None:intergenic 20.0%
! AGCATAATATTAATGAAACG+CGG - chr7.3:70286320-70286339 MS.gene62523:CDS 25.0%
! ATCTACAGATTGGAAATTTA+GGG - chr7.3:70285320-70285339 MS.gene62523:CDS 25.0%
! ATGCATAGTTCAAACTTAAA+TGG + chr7.3:70285181-70285200 None:intergenic 25.0%
! ATTTAAAAGCAGAGGTTAAT+TGG + chr7.3:70287188-70287207 None:intergenic 25.0%
! GAAATCAAAGAAGTCATATT+TGG - chr7.3:70286530-70286549 MS.gene62523:CDS 25.0%
! GACATAAATCATCTAAAAAG+CGG - chr7.3:70286710-70286729 MS.gene62523:intron 25.0%
! TATCTACAGATTGGAAATTT+AGG - chr7.3:70285319-70285338 MS.gene62523:CDS 25.0%
! TGAATTCATTTGGAAAAACT+TGG - chr7.3:70285997-70286016 MS.gene62523:CDS 25.0%
!! AAAAAGCATGAAAATGTGAA+GGG - chr7.3:70286427-70286446 MS.gene62523:CDS 25.0%
!! AAGGAATCTATTGATAACAA+TGG - chr7.3:70284844-70284863 MS.gene62523:CDS 25.0%
!! ATATGACTTCTTTGATTTCT+TGG + chr7.3:70286529-70286548 None:intergenic 25.0%
!! GTGATATATATGTTGATAAG+AGG - chr7.3:70285742-70285761 MS.gene62523:CDS 25.0%
!! TTTGTTTCAAATCTGGAATT+AGG + chr7.3:70285949-70285968 None:intergenic 25.0%
!!! AATTAACCTCTGCTTTTAAA+TGG - chr7.3:70287187-70287206 MS.gene62523:intron 25.0%
!!! AATTTTTGTTCGAGTTTGTT+CGG - chr7.3:70287091-70287110 MS.gene62523:CDS 25.0%
!!! TTTCATGCTTTTTCTTATCT+TGG + chr7.3:70286420-70286439 None:intergenic 25.0%
AAAAAACAAGCTCAGATAAC+CGG + chr7.3:70287527-70287546 None:intergenic 30.0%
AAAATTCATATCACAGCTCT+TGG + chr7.3:70287583-70287602 None:intergenic 30.0%
AACAAAGGAAGGGATTATAA+AGG - chr7.3:70284793-70284812 MS.gene62523:CDS 30.0%
ACTAATTTCTCTTGCAATGA+AGG - chr7.3:70285911-70285930 MS.gene62523:CDS 30.0%
ATGTTCTTTACCAGATAACT+CGG + chr7.3:70286263-70286282 None:intergenic 30.0%
ATTCCTTCTTCTTCTCAATA+TGG + chr7.3:70284831-70284850 None:intergenic 30.0%
GGATTGCTTAAATTGTGAAA+CGG + chr7.3:70286241-70286260 None:intergenic 30.0%
GTAAATGTTTCACTGTTTGA+TGG - chr7.3:70285854-70285873 MS.gene62523:CDS 30.0%
TCAAAGAAGTCATATTTGGA+AGG - chr7.3:70286534-70286553 MS.gene62523:CDS 30.0%
TCTTGATCTTATCATCAGAA+GGG + chr7.3:70286771-70286790 None:intergenic 30.0%
TGCTAGTATACTACTAACTT+GGG - chr7.3:70286183-70286202 MS.gene62523:CDS 30.0%
TTATATCGTCATCAACAAGA+AGG - chr7.3:70285971-70285990 MS.gene62523:CDS 30.0%
TTCAAAGAAACAAGCATTCT+TGG - chr7.3:70286364-70286383 MS.gene62523:CDS 30.0%
TTCTTGATCTTATCATCAGA+AGG + chr7.3:70286772-70286791 None:intergenic 30.0%
TTTCCTTGAATGTTCTCTTT+CGG - chr7.3:70286993-70287012 MS.gene62523:CDS 30.0%
! AAAAAGTTTTTGCCATAAGC+AGG - chr7.3:70286687-70286706 MS.gene62523:intron 30.0%
! AAAAGCATGAAAATGTGAAG+GGG - chr7.3:70286428-70286447 MS.gene62523:CDS 30.0%
! AAAAGTTTTTGCCATAAGCA+GGG - chr7.3:70286688-70286707 MS.gene62523:intron 30.0%
! CTTAAATGGTCTTAAAATGG+AGG + chr7.3:70285167-70285186 None:intergenic 30.0%
! GAAAAAGCATGAAAATGTGA+AGG - chr7.3:70286426-70286445 MS.gene62523:CDS 30.0%
! TAATTAATTGTAAAGGCGCA+TGG + chr7.3:70287161-70287180 None:intergenic 30.0%
!! AACAATAATCGGAAAGCATT+TGG + chr7.3:70286158-70286177 None:intergenic 30.0%
!! AAGAAGGCTTTGAATTCATT+TGG - chr7.3:70285987-70286006 MS.gene62523:CDS 30.0%
!! ACAATAATCGGAAAGCATTT+GGG + chr7.3:70286157-70286176 None:intergenic 30.0%
!! TCAAAAGCACACATTTTATG+CGG - chr7.3:70284881-70284900 MS.gene62523:CDS 30.0%
!!! CTTGGTTACTTTTCTGATTT+TGG - chr7.3:70285519-70285538 MS.gene62523:CDS 30.0%
!!! TTGTTTTGACACCTTCATAT+GGG + chr7.3:70287071-70287090 None:intergenic 30.0%
!!! TTTGTTTTGACACCTTCATA+TGG + chr7.3:70287072-70287091 None:intergenic 30.0%
AAAATAAGCTTCCTGGGTTA+AGG - chr7.3:70286845-70286864 MS.gene62523:intron 35.0%
AAATAAGCTTCCTGGGTTAA+GGG - chr7.3:70286846-70286865 MS.gene62523:intron 35.0%
AATTTGCATCGTTGAAGCTA+TGG + chr7.3:70287278-70287297 None:intergenic 35.0%
ACTATGGAGGAGGAAAAATA+TGG - chr7.3:70287483-70287502 MS.gene62523:CDS 35.0%
ATATGCCTTGAGAAACACTA+TGG + chr7.3:70286122-70286141 None:intergenic 35.0%
ATATGGTAAACAGGCTGAAA+AGG - chr7.3:70287500-70287519 MS.gene62523:CDS 35.0%
ATATTGCAAATTGTGTGGTG+TGG - chr7.3:70285426-70285445 MS.gene62523:CDS 35.0%
ATCAAGTAAACAGAGAATGG+TGG - chr7.3:70284768-70284787 MS.gene62523:CDS 35.0%
ATCCTTGAAAATAAGCTTCC+TGG - chr7.3:70286838-70286857 MS.gene62523:intron 35.0%
ATCGATATGGAACCTTAAGA+AGG - chr7.3:70285041-70285060 MS.gene62523:CDS 35.0%
ATGATTTATGTCCCTGCTTA+TGG + chr7.3:70286702-70286721 None:intergenic 35.0%
CAAATATATCTCACCACTAG+TGG - chr7.3:70285462-70285481 MS.gene62523:CDS 35.0%
CAAGAAGCTGAAATTGCTAA+TGG - chr7.3:70286786-70286805 MS.gene62523:intron 35.0%
CAAGTTAGTAGTATACTAGC+AGG + chr7.3:70286184-70286203 None:intergenic 35.0%
CACAGTTGAATCATTCTTCA+TGG + chr7.3:70284950-70284969 None:intergenic 35.0%
CATCCATATTGAGAAGAAGA+AGG - chr7.3:70284825-70284844 MS.gene62523:CDS 35.0%
CTGCTAGTATACTACTAACT+TGG - chr7.3:70286182-70286201 MS.gene62523:CDS 35.0%
CTTAAAATGGAGGATTACCA+TGG + chr7.3:70285157-70285176 None:intergenic 35.0%
GAATTCAAGAAATGGGACAT+CGG - chr7.3:70286211-70286230 MS.gene62523:CDS 35.0%
GGAATCAAGTAAACAGAGAA+TGG - chr7.3:70284765-70284784 MS.gene62523:CDS 35.0%
GGAGGAAAAATATGGTAAAC+AGG - chr7.3:70287491-70287510 MS.gene62523:CDS 35.0%
GGAGGATGAATTCAAGAAAT+GGG - chr7.3:70286204-70286223 MS.gene62523:CDS 35.0%
GGATGACAAAAATGATTTCG+TGG - chr7.3:70286341-70286360 MS.gene62523:CDS 35.0%
GGTGTATATTGCAAATTGTG+TGG - chr7.3:70285421-70285440 MS.gene62523:CDS 35.0%
TAGTATACTACTAACTTGGG+AGG - chr7.3:70286186-70286205 MS.gene62523:CDS 35.0%
TCCATTCCAGAACAATTTCT+GGG - chr7.3:70286603-70286622 MS.gene62523:CDS 35.0%
TCCTTGAAAATAAGCTTCCT+GGG - chr7.3:70286839-70286858 MS.gene62523:intron 35.0%
TCTTAAGGTTCCATATCGAT+GGG + chr7.3:70285041-70285060 None:intergenic 35.0%
TGTTCTTTACCAGATAACTC+GGG + chr7.3:70286262-70286281 None:intergenic 35.0%
TTCATTTGGAAAAACTTGGC+AGG - chr7.3:70286001-70286020 MS.gene62523:CDS 35.0%
TTCTTAAGGTTCCATATCGA+TGG + chr7.3:70285042-70285061 None:intergenic 35.0%
TTGTACTTCTGGATGTTGTA+TGG - chr7.3:70287370-70287389 MS.gene62523:CDS 35.0%
! ATCGTTGAAGCTATGGATTA+CGG + chr7.3:70287271-70287290 None:intergenic 35.0%
! ATCTACAAAAGCTTGTTCTG+AGG - chr7.3:70287320-70287339 MS.gene62523:CDS 35.0%
! GTGTGTTACTATCTACAGAT+TGG - chr7.3:70285310-70285329 MS.gene62523:CDS 35.0%
! TCATCTAGAACTAACAAACC+AGG + chr7.3:70286496-70286515 None:intergenic 35.0%
! TCTACAAAAGCTTGTTCTGA+GGG - chr7.3:70287321-70287340 MS.gene62523:CDS 35.0%
! TTTTAAATGGTCTGAGGGAA+AGG - chr7.3:70287200-70287219 MS.gene62523:intron 35.0%
!! GGTTTGTTAGTTCTAGATGA+AGG - chr7.3:70286496-70286515 MS.gene62523:CDS 35.0%
!! TCTGCTTTTAAATGGTCTGA+GGG - chr7.3:70287195-70287214 MS.gene62523:intron 35.0%
!! TGCAAAGTATTGTGTACTTG+CGG - chr7.3:70285598-70285617 MS.gene62523:CDS 35.0%
!!! CAAAAACTTTTTAGCTCAGG+CGG + chr7.3:70286679-70286698 None:intergenic 35.0%
!!! TGGCAAAAACTTTTTAGCTC+AGG + chr7.3:70286682-70286701 None:intergenic 35.0%
AAAGCTCCCAGAAATTGTTC+TGG + chr7.3:70286612-70286631 None:intergenic 40.0%
AAGATTCTGCTAAAAGCTCC+TGG - chr7.3:70286475-70286494 MS.gene62523:CDS 40.0%
AGAACGCTTCACTAAGCTTA+AGG + chr7.3:70286641-70286660 None:intergenic 40.0%
ATGCGAATAGAACCTTTGCT+TGG + chr7.3:70287302-70287321 None:intergenic 40.0%
ATTCAAGGAAAAGCGATGGA+TGG + chr7.3:70286984-70287003 None:intergenic 40.0%
CCTTTGTGCACATGAACAAA+GGG + chr7.3:70286814-70286833 None:intergenic 40.0%
CCTTTGTTCATGTGCACAAA+GGG - chr7.3:70286812-70286831 MS.gene62523:intron 40.0%
CTAGCAGGAGCAACAATAAT+CGG + chr7.3:70286169-70286188 None:intergenic 40.0%
CTCAGACCATTTAAAAGCAG+AGG + chr7.3:70287196-70287215 None:intergenic 40.0%
CTCCATTCCAGAACAATTTC+TGG - chr7.3:70286602-70286621 MS.gene62523:CDS 40.0%
CTTAAGGTTCCATATCGATG+GGG + chr7.3:70285040-70285059 None:intergenic 40.0%
CTTAGACTGAACCCATATGA+AGG - chr7.3:70287057-70287076 MS.gene62523:CDS 40.0%
CTTCCGAAAGAGAACATTCA+AGG + chr7.3:70286999-70287018 None:intergenic 40.0%
CTTGGACTCATCGTCATTAT+CGG + chr7.3:70287565-70287584 None:intergenic 40.0%
CTTTGTTCATGTGCACAAAG+GGG - chr7.3:70286813-70286832 MS.gene62523:intron 40.0%
GAAAATGTGAAGGGGAGAAA+AGG - chr7.3:70286436-70286455 MS.gene62523:CDS 40.0%
GAACATTCAAGGAAAAGCGA+TGG + chr7.3:70286988-70287007 None:intergenic 40.0%
GATATGGAACCTTAAGAAGG+TGG - chr7.3:70285044-70285063 MS.gene62523:CDS 40.0%
GGGAGGATGAATTCAAGAAA+TGG - chr7.3:70286203-70286222 MS.gene62523:CDS 40.0%
TAGCTTAGTTGGAGCTTCTA+GGG - chr7.3:70287347-70287366 MS.gene62523:CDS 40.0%
TGGTAAAGAACATGCTGATG+CGG - chr7.3:70286270-70286289 MS.gene62523:CDS 40.0%
TTAAGCAATCCCGAGTTATC+TGG - chr7.3:70286250-70286269 MS.gene62523:CDS 40.0%
TTAAGGTTCCATATCGATGG+GGG + chr7.3:70285039-70285058 None:intergenic 40.0%
TTAGCTTAGTTGGAGCTTCT+AGG - chr7.3:70287346-70287365 MS.gene62523:CDS 40.0%
TTTCTTCCTGAACCTTCACA+GGG + chr7.3:70285818-70285837 None:intergenic 40.0%
! AAAAGTGCAAAAGACCATGG+AGG - chr7.3:70285068-70285087 MS.gene62523:CDS 40.0%
! AATGGTGGAGAACAAAGGAA+GGG - chr7.3:70284783-70284802 MS.gene62523:CDS 40.0%
! ACTTTTCTCGACGAAGAGAT+TGG - chr7.3:70285400-70285419 MS.gene62523:CDS 40.0%
! AGACATGTTTGTTAAGTGCG+TGG - chr7.3:70287647-70287666 MS.gene62523:CDS 40.0%
! ATAGCTTGTCTTTCGACTGA+AGG + chr7.3:70287399-70287418 None:intergenic 40.0%
! ATTACGGATTGCTTGACCTT+TGG + chr7.3:70287255-70287274 None:intergenic 40.0%
! ATTGTAAAGGCGCATGGAAT+TGG + chr7.3:70287155-70287174 None:intergenic 40.0%
! CGAAGTGTTATGGGATGAAT+TGG - chr7.3:70285101-70285120 MS.gene62523:CDS 40.0%
! GGAAAAAGTGCAAAAGACCA+TGG - chr7.3:70285065-70285084 MS.gene62523:CDS 40.0%
! GGAATCAGCTACAGTTTGTT+TGG - chr7.3:70286385-70286404 MS.gene62523:CDS 40.0%
! GTTGATAGATTTCCCTGTGA+AGG - chr7.3:70285803-70285822 MS.gene62523:CDS 40.0%
! TCTGAGGGAATTAGCTTAGT+TGG - chr7.3:70287336-70287355 MS.gene62523:CDS 40.0%
! TGCATTATTTCTCATGCTCC+TGG - chr7.3:70286076-70286095 MS.gene62523:CDS 40.0%
! TTCAACTGTGTTCGAGTTCA+AGG - chr7.3:70284960-70284979 MS.gene62523:CDS 40.0%
! TTGCAATGAAGGCACAGTTT+GGG - chr7.3:70285922-70285941 MS.gene62523:CDS 40.0%
!! AAGGAAGTGTTGTTCATGTC+TGG - chr7.3:70287219-70287238 MS.gene62523:intron 40.0%
!! AATCGGAAAGCATTTGGGAA+AGG + chr7.3:70286152-70286171 None:intergenic 40.0%
!! ACCCAGGAAGCTTATTTTCA+AGG + chr7.3:70286843-70286862 None:intergenic 40.0%
!! ATTGTTCTGGAATGGAGTTC+CGG + chr7.3:70286599-70286618 None:intergenic 40.0%
!! CTCTGCTTTTAAATGGTCTG+AGG - chr7.3:70287194-70287213 MS.gene62523:intron 40.0%
!! GGAAGAAAGGCATCATTTGA+TGG - chr7.3:70285830-70285849 MS.gene62523:CDS 40.0%
!! TCCCAGAAATTGTTCTGGAA+TGG + chr7.3:70286607-70286626 None:intergenic 40.0%
!!! ACTTGTTTTACACGTGACAC+AGG - chr7.3:70285781-70285800 MS.gene62523:CDS 40.0%
!!! GCACTTTTTCCACCTTCTTA+AGG + chr7.3:70285056-70285075 None:intergenic 40.0%
AAGGAAAAGCGATGGATGGA+CGG + chr7.3:70286980-70286999 None:intergenic 45.0%
AGAAGAATCTGCTCTCGACA+AGG - chr7.3:70287020-70287039 MS.gene62523:CDS 45.0%
AGAATCTGACGCTGTATCCA+TGG - chr7.3:70285137-70285156 MS.gene62523:CDS 45.0%
AGATTTCCCTGTGAAGGTTC+AGG - chr7.3:70285809-70285828 MS.gene62523:CDS 45.0%
ATTTCTCATGCTCCTGGAAC+CGG - chr7.3:70286082-70286101 MS.gene62523:CDS 45.0%
CAGAAGCGAATCATCCTTTC+CGG - chr7.3:70286577-70286596 MS.gene62523:CDS 45.0%
CCCTTTGTGCACATGAACAA+AGG + chr7.3:70286815-70286834 None:intergenic 45.0%
CCCTTTGTTCATGTGCACAA+AGG - chr7.3:70286811-70286830 MS.gene62523:intron 45.0%
CGATGCAAATTGCCAAGCAA+AGG - chr7.3:70287287-70287306 MS.gene62523:intron 45.0%
CTGTGAAGGTTCAGGAAGAA+AGG - chr7.3:70285817-70285836 MS.gene62523:CDS 45.0%
CTTTCTTCCTGAACCTTCAC+AGG + chr7.3:70285819-70285838 None:intergenic 45.0%
GCGGCTAAAGCAGTACTATT+TGG - chr7.3:70285617-70285636 MS.gene62523:CDS 45.0%
GCTGACCATAGTGTTTCTCA+AGG - chr7.3:70286114-70286133 MS.gene62523:CDS 45.0%
GTAAACAGGCTGAAAAGGAC+CGG - chr7.3:70287505-70287524 MS.gene62523:CDS 45.0%
TCTGACCAATCCTATACGCT+CGG + chr7.3:70287425-70287444 None:intergenic 45.0%
TTGTTAAGTGCGTGGTACTG+AGG - chr7.3:70287655-70287674 MS.gene62523:CDS 45.0%
! AACTGTGTTCGAGTTCAAGG+AGG - chr7.3:70284963-70284982 MS.gene62523:CDS 45.0%
! AGGAGGAGAACGAAGTGTTA+TGG - chr7.3:70285091-70285110 MS.gene62523:CDS 45.0%
! CAGAGAATGGTGGAGAACAA+AGG - chr7.3:70284778-70284797 MS.gene62523:CDS 45.0%
! CTTGCAATGAAGGCACAGTT+TGG - chr7.3:70285921-70285940 MS.gene62523:CDS 45.0%
! GAATGGTGGAGAACAAAGGA+AGG - chr7.3:70284782-70284801 MS.gene62523:CDS 45.0%
! GGACTGTTTGGTGACTTTGA+AGG - chr7.3:70286867-70286886 MS.gene62523:intron 45.0%
! GGAGGAGAACGAAGTGTTAT+GGG - chr7.3:70285092-70285111 MS.gene62523:CDS 45.0%
! TATGGTCAGCCTTGTCTTTC+CGG + chr7.3:70286104-70286123 None:intergenic 45.0%
! TGTTTGGTGACTTTGAAGGC+AGG - chr7.3:70286871-70286890 MS.gene62523:intron 45.0%
!! AGCTTCTAGGGTTGTACTTC+TGG - chr7.3:70287359-70287378 MS.gene62523:CDS 45.0%
!! TAGGATTGGTCAGAAGAGAG+TGG - chr7.3:70287431-70287450 MS.gene62523:CDS 45.0%
AAGCTATCAGCCGAGCGTAT+AGG - chr7.3:70287412-70287431 MS.gene62523:CDS 50.0%
AATGCCGATCCTTGCAGTGA+AGG - chr7.3:70286049-70286068 MS.gene62523:CDS 50.0%
ACATACCATCTGCTCGCTGA+AGG - chr7.3:70287459-70287478 MS.gene62523:CDS 50.0%
ACCAAACAGTCCCTTAACCC+AGG + chr7.3:70286859-70286878 None:intergenic 50.0%
ACTGAGGAAGGAGCGAGATA+TGG - chr7.3:70287671-70287690 MS.gene62523:CDS 50.0%
AGTGCAAAAGACCATGGAGG+AGG - chr7.3:70285071-70285090 MS.gene62523:CDS 50.0%
ATCAGCCGAGCGTATAGGAT+TGG - chr7.3:70287417-70287436 MS.gene62523:CDS 50.0%
CATACCATCTGCTCGCTGAA+GGG - chr7.3:70287460-70287479 MS.gene62523:CDS 50.0%
CGGAGTTGTGAACCTGTTTC+GGG - chr7.3:70286730-70286749 MS.gene62523:intron 50.0%
CTCACCACTAGTGGTAAGCA+CGG - chr7.3:70285471-70285490 MS.gene62523:CDS 50.0%
GAAACCGTGCTTACCACTAG+TGG + chr7.3:70285478-70285497 None:intergenic 50.0%
GCGGAGTTGTGAACCTGTTT+CGG - chr7.3:70286729-70286748 MS.gene62523:intron 50.0%
GCTCCTTGCGCAATGCTATT+GGG - chr7.3:70284912-70284931 MS.gene62523:CDS 50.0%
GGACTCATCGTCATTATCGG+CGG + chr7.3:70287562-70287581 None:intergenic 50.0%
GTCTGGCAAAGATCCTCCAA+AGG - chr7.3:70287236-70287255 MS.gene62523:intron 50.0%
TCCTGGGTTAAGGGACTGTT+TGG - chr7.3:70286855-70286874 MS.gene62523:intron 50.0%
TCGTGTTCACATCGCGATGA+TGG + chr7.3:70285379-70285398 None:intergenic 50.0%
TGCTCCTTGCGCAATGCTAT+TGG - chr7.3:70284911-70284930 MS.gene62523:CDS 50.0%
TGTCCCAATAGCATTGCGCA+AGG + chr7.3:70284918-70284937 None:intergenic 50.0%
! ACGGATTGCTTGACCTTTGG+AGG + chr7.3:70287252-70287271 None:intergenic 50.0%
! CTTGTTGTGTTGCCCGAAAC+AGG + chr7.3:70286745-70286764 None:intergenic 50.0%
! GTGTTCGAGTTCAAGGAGGA+TGG - chr7.3:70284967-70284986 MS.gene62523:CDS 50.0%
! TAAGTGCGTGGTACTGAGGA+AGG - chr7.3:70287659-70287678 MS.gene62523:CDS 50.0%
! TCTGGAATGGAGTTCCGGAA+AGG + chr7.3:70286594-70286613 None:intergenic 50.0%
ACAAGCTACACAGCCACCAC+CGG - chr7.3:70284999-70285018 MS.gene62523:CDS 55.0%
AGCTCAGGCGGTATCGTGTT+AGG + chr7.3:70286667-70286686 None:intergenic 55.0%
ATGCAGCCACCTTCACTGCA+AGG + chr7.3:70286061-70286080 None:intergenic 55.0%
CTCCTGGAACCGGAAAGACA+AGG - chr7.3:70286092-70286111 MS.gene62523:CDS 55.0%
GCAAAAGACCATGGAGGAGG+AGG - chr7.3:70285074-70285093 MS.gene62523:CDS 55.0%
GCCACCTTCACTGCAAGGAT+CGG + chr7.3:70286056-70286075 None:intergenic 55.0%
TAGTCCCTTCAGCGAGCAGA+TGG + chr7.3:70287467-70287486 None:intergenic 55.0%
TCTGCTCGCTGAAGGGACTA+TGG - chr7.3:70287467-70287486 MS.gene62523:CDS 55.0%
TGCTCTCGACAAGGATCAGC+TGG - chr7.3:70287029-70287048 MS.gene62523:CDS 55.0%
TGGAGACACCCCCATCGATA+TGG - chr7.3:70285028-70285047 MS.gene62523:CDS 55.0%
TTCGTTCTCCTCCTCCTCCA+TGG + chr7.3:70285085-70285104 None:intergenic 55.0%
! AGCCTTGTCTTTCCGGTTCC+AGG + chr7.3:70286097-70286116 None:intergenic 55.0%
CGCTGAAGGGACTATGGAGG+AGG - chr7.3:70287473-70287492 MS.gene62523:CDS 60.0%
GCCGATCCTTGCAGTGAAGG+TGG - chr7.3:70286052-70286071 MS.gene62523:CDS 60.0%
GCTCGCTGAAGGGACTATGG+AGG - chr7.3:70287470-70287489 MS.gene62523:CDS 60.0%
GGTGTCTCCACAGAAACCGG+TGG + chr7.3:70285018-70285037 None:intergenic 60.0%
GTCTCCACAGAAACCGGTGG+TGG + chr7.3:70285015-70285034 None:intergenic 60.0%
!! ACAGCCACCACCGGTTTCTG+TGG - chr7.3:70285008-70285027 MS.gene62523:CDS 60.0%
!! GGGGGTGTCTCCACAGAAAC+CGG + chr7.3:70285021-70285040 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 70284757 70287698 70284757 ID=MS.gene62523
chr7.3 mRNA 70284757 70287698 70284757 ID=MS.gene62523.t1;Parent=MS.gene62523
chr7.3 exon 70287297 70287698 70287297 ID=MS.gene62523.t1.exon1;Parent=MS.gene62523.t1
chr7.3 CDS 70287297 70287698 70287297 ID=cds.MS.gene62523.t1;Parent=MS.gene62523.t1
chr7.3 exon 70286972 70287127 70286972 ID=MS.gene62523.t1.exon2;Parent=MS.gene62523.t1
chr7.3 CDS 70286972 70287127 70286972 ID=cds.MS.gene62523.t1;Parent=MS.gene62523.t1
chr7.3 exon 70284757 70286652 70284757 ID=MS.gene62523.t1.exon3;Parent=MS.gene62523.t1
chr7.3 CDS 70284757 70286652 70284757 ID=cds.MS.gene62523.t1;Parent=MS.gene62523.t1
Gene Sequence

>MS.gene62523

ATGATGGAGGAATCAAGTAAACAGAGAATGGTGGAGAACAAAGGAAGGGATTATAAAGGTATAGCTAACATCCATATTGAGAAGAAGAAGGAATCTATTGATAACAATGGTTTGATACAAAGCGTCAAAAGCACACATTTTATGCGGAATGAGTTGCTCCTTGCGCAATGCTATTGGGACAGAAAGAATACCATGAAGAATGATTCAACTGTGTTCGAGTTCAAGGAGGATGGCGTTGATCGACAAGCTACACAGCCACCACCGGTTTCTGTGGAGACACCCCCATCGATATGGAACCTTAAGAAGGTGGAAAAAGTGCAAAAGACCATGGAGGAGGAGGAGAACGAAGTGTTATGGGATGAATTGGATACAGTTCTTAGAGAATCTGACGCTGTATCCATGGTAATCCTCCATTTTAAGACCATTTAAGTTTGAACTATGCATTTTAATTTTGTGAACTATAGGACTAGTGCTCAAGCTGATTTTAATTTTTATTTTAAACTGTTTATATGTTTAAACTTCGGTCTAAGCAATATCTATATGTTTATACATAGTGTGTTACTATCTACAGATTGGAAATTTAGGGACAAATGAAGCTACAAAAATAAAAAACGGAAGTCCATCATCGCGATGTGAACACGACACTTTTCTCGACGAAGAGATTGGTGTATATTGCAAATTGTGTGGTGTGGTTATTACTGAGATCAAATATATCTCACCACTAGTGGTAAGCACGGTTTCATATTGATTTATGTAAAATAACTTGGTTACTTTTCTGATTTTGGTCTTGAATAGTGAAATTTGAACACATCACTTTTGATTGCATTATGCAGCGATTAAATGCAAAGTATTGTGTACTTGCGGCTAAAGCAGTACTATTTGGAACATAACACTAATAAATTTGTTGCTGTTAGATTCAATAAGTTTCTGCTGTTAGATTCAAATTAAATTTCATATAGTTGTATGTTTGTTCTGATTTTACCTTGTGATATATATGTTGATAAGAGGCACTGATATTTTGCATACTTGTTTTACACGTGACACAGGTTGATAGATTTCCCTGTGAAGGTTCAGGAAGAAAGGCATCATTTGATGGAGTAAATGTTTCACTGTTTGATGGATCTCAATTCAATGTTTATGATAAAGATTCAGAAACTAATTTCTCTTGCAATGAAGGCACAGTTTGGGACCTAATTCCAGATTTGAAACAAACTTTATATCGTCATCAACAAGAAGGCTTTGAATTCATTTGGAAAAACTTGGCAGGAAGTGTCAAGCTTCAAAAGTTAAAGAATGCCGATCCTTGCAGTGAAGGTGGCTGCATTATTTCTCATGCTCCTGGAACCGGAAAGACAAGGCTGACCATAGTGTTTCTCAAGGCATATTTGAAAGCCTTTCCCAAATGCTTTCCGATTATTGTTGCTCCTGCTAGTATACTACTAACTTGGGAGGATGAATTCAAGAAATGGGACATCGGAGTTCCGTTTCACAATTTAAGCAATCCCGAGTTATCTGGTAAAGAACATGCTGATGCGGTTGAAACGTTTGATCAGTATAATACACAGCATAATATTAATGAAACGCGGATGACAAAAATGATTTCGTGGTTCAAAGAAACAAGCATTCTTGGAATCAGCTACAGTTTGTTTGGAAAAAAATGCCAAGATAAGAAAAAGCATGAAAATGTGAAGGGGAGAAAAGGAAACTGTGATATGCGAAAGATTCTGCTAAAAGCTCCTGGTTTGTTAGTTCTAGATGAAGGACACACACCAAGAAATCAAAGAAGTCATATTTGGAAGGTGTTGTCGAAGATTCAAGCGCAGAAGCGAATCATCCTTTCCGGAACTCCATTCCAGAACAATTTCTGGGAGCTTTACAGCACCTTAAGCTTAGTGAAGCGTTCTTTTCCTAACACGATACCGCCTGAGCTAAAAAGTTTTTGCCATAAGCAGGGACATAAATCATCTAAAAAGCGGAGTTGTGAACCTGTTTCGGGCAACACAACAAGAGACCCTTCTGATGATAAGATCAAGAAGCTGAAATTGCTAATGGATCCCTTTGTTCATGTGCACAAAGGGGCGATCCTTGAAAATAAGCTTCCTGGGTTAAGGGACTGTTTGGTGACTTTGAAGGCAGGTAGTTTGCAGAATGAAATTCTGAAGAGCATTAAACGTTCTAAGAACACAATATTTAACTTTGAACGTAAGATAGCATTGACATCCGTCCATCCATCGCTTTTCCTTGAATGTTCTCTTTCGGAAGAAGAAGAATCTGCTCTCGACAAGGATCAGCTGGAAAAGCTTAGACTGAACCCATATGAAGGTGTCAAAACAAAATTTTTGTTCGAGTTTGTTCGGCTCTGCGATGCTTTTCACGAGAAAGTTCTTGTGTTCAGCCAATTCCATGCGCCTTTACAATTAATTAAAGACCAATTAACCTCTGCTTTTAAATGGTCTGAGGGAAAGGAAGTGTTGTTCATGTCTGGCAAAGATCCTCCAAAGGTCAAGCAATCCGTAATCCATAGCTTCAACGATGCAAATTGCCAAGCAAAGGTTCTATTCGCATCTACAAAAGCTTGTTCTGAGGGAATTAGCTTAGTTGGAGCTTCTAGGGTTGTACTTCTGGATGTTGTATGGAATCCTTCAGTCGAAAGACAAGCTATCAGCCGAGCGTATAGGATTGGTCAGAAGAGAGTGGTATACACATACCATCTGCTCGCTGAAGGGACTATGGAGGAGGAAAAATATGGTAAACAGGCTGAAAAGGACCGGTTATCTGAGCTTGTTTTTTCAGAGAAAAATGCCGCCGATAATGACGATGAGTCCAAGAGCTGTGATATGAATTTTGAAGATAGAGTTCTTGATCATATGACTCAGCATGAAAATCTCGAAGACATGTTTGTTAAGTGCGTGGTACTGAGGAAGGAGCGAGATATGGTCTGA
Protein sequence

>MS.gene62523.t1

MMEESSKQRMVENKGRDYKGIANIHIEKKKESIDNNGLIQSVKSTHFMRNELLLAQCYWDRKNTMKNDSTVFEFKEDGVDRQATQPPPVSVETPPSIWNLKKVEKVQKTMEEEENEVLWDELDTVLRESDAVSMIGNLGTNEATKIKNGSPSSRCEHDTFLDEEIGVYCKLCGVVITEIKYISPLVVDRFPCEGSGRKASFDGVNVSLFDGSQFNVYDKDSETNFSCNEGTVWDLIPDLKQTLYRHQQEGFEFIWKNLAGSVKLQKLKNADPCSEGGCIISHAPGTGKTRLTIVFLKAYLKAFPKCFPIIVAPASILLTWEDEFKKWDIGVPFHNLSNPELSGKEHADAVETFDQYNTQHNINETRMTKMISWFKETSILGISYSLFGKKCQDKKKHENVKGRKGNCDMRKILLKAPGLLVLDEGHTPRNQRSHIWKVLSKIQAQKRIILSGTPFQNNFWELYSTLSLVKRSFPNTIPPELKSFCHKQGHKSSKKRSCEPVSGNTTRDPSDDKIKKLKLLMDPFVHVHKGAILENKLPGLRDCLVTLKAGSLQNEILKSIKRSKNTIFNFERKIALTSVHPSLFLECSLSEEEESALDKDQLEKLRLNPYEGVKTKFLFEFVRLCDAFHEKVLVFSQFHAPLQLIKDQLTSAFKWSEGKEVLFMSGKDPPKVKQSVIHSFNDANCQAKVLFASTKACSEGISLVGASRVVLLDVVWNPSVERQAISRAYRIGQKRVVYTYHLLAEGTMEEEKYGKQAEKDRLSELVFSEKNAADNDDESKSCDMNFEDRVLDHMTQHENLEDMFVKCVVLRKERDMV