AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62524

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62524.t1 MTR_7g029525 94.125 817 48 0 1 817 508 1324 0.0 1566
MS.gene62524.t1 MTR_7g450790 83.272 819 127 4 4 817 626 1439 0.0 1363
MS.gene62524.t1 MTR_7g450790 65.278 72 25 0 61 132 461 532 2.00e-20 97.8
MS.gene62524.t1 MTR_4g088650 79.659 821 133 4 2 817 427 1218 0.0 1288
MS.gene62524.t1 MTR_7g450780 67.571 848 231 14 2 817 511 1346 0.0 1065
MS.gene62524.t1 MTR_7g450780 64.384 73 26 0 61 133 387 459 2.34e-19 94.4
MS.gene62524.t1 MTR_7g450820 67.028 831 246 10 2 816 416 1234 0.0 1063
MS.gene62524.t1 MTR_7g050445 66.187 834 248 13 2 817 481 1298 0.0 1060
MS.gene62524.t1 MTR_7g450860 65.306 833 255 13 4 816 454 1272 0.0 1020
MS.gene62524.t1 MTR_2g084695 67.514 354 43 2 464 817 9 290 8.54e-151 443
MS.gene62524.t1 MTR_1g081750 32.986 767 440 18 76 798 363 1099 2.71e-117 382
MS.gene62524.t1 MTR_7g026650 31.865 772 463 17 82 817 408 1152 1.89e-110 365
MS.gene62524.t1 MTR_3g071860 32.922 729 431 17 111 798 539 1250 2.35e-108 360
MS.gene62524.t1 MTR_5g083300 30.702 798 493 17 43 798 508 1287 1.49e-104 350
MS.gene62524.t1 MTR_5g083300 30.702 798 493 17 43 798 545 1324 2.44e-104 350
MS.gene62524.t1 MTR_8g037345 29.063 726 427 18 111 810 234 897 4.30e-87 296
MS.gene62524.t1 MTR_2g437480 31.378 682 376 18 111 767 387 1001 9.23e-87 297
MS.gene62524.t1 MTR_7g026620 25.997 577 298 16 155 713 177 642 1.13e-45 174
MS.gene62524.t1 MTR_5g004720 24.493 592 351 15 216 771 161 692 1.05e-35 146
MS.gene62524.t1 MTR_8g094070 25.043 583 331 20 231 770 373 892 1.78e-28 123
MS.gene62524.t1 MTR_8g094070 25.043 583 331 20 231 770 373 892 1.83e-28 123
MS.gene62524.t1 MTR_1g069755 24.490 588 327 21 222 767 371 883 3.39e-27 119
MS.gene62524.t1 MTR_3g106210 23.921 556 343 13 278 802 309 815 5.23e-25 112
MS.gene62524.t1 MTR_3g106210 23.921 556 343 13 278 802 309 815 5.75e-25 112
MS.gene62524.t1 MTR_3g106210 23.921 556 343 13 278 802 309 815 6.34e-25 112
MS.gene62524.t1 MTR_3g106210 23.921 556 343 13 278 802 309 815 6.35e-25 112
MS.gene62524.t1 MTR_1g013190 23.211 629 392 19 205 794 228 804 5.03e-24 109
MS.gene62524.t1 MTR_1g035330 25.520 529 304 20 276 753 215 704 1.83e-23 106
MS.gene62524.t1 MTR_5g020000 23.656 558 327 17 279 806 206 694 6.16e-23 105
MS.gene62524.t1 MTR_4g096930 25.862 348 207 11 419 750 870 1182 3.39e-22 103
MS.gene62524.t1 MTR_1g080420 37.267 161 93 2 614 766 984 1144 6.76e-22 102
MS.gene62524.t1 MTR_1g080420 37.267 161 93 2 614 766 1115 1275 7.04e-22 102
MS.gene62524.t1 MTR_2g020000 24.366 513 294 15 279 774 213 648 2.29e-19 94.0
MS.gene62524.t1 MTR_4g118845 24.863 366 233 9 420 775 126 459 2.44e-19 94.0
MS.gene62524.t1 MTR_3g053910 22.686 551 325 16 243 772 571 1041 3.61e-19 94.0
MS.gene62524.t1 MTR_3g053910 22.686 551 325 16 243 772 600 1070 3.91e-19 93.6
MS.gene62524.t1 MTR_4g118845 25.067 371 224 10 420 774 313 645 4.94e-19 93.2
MS.gene62524.t1 MTR_5g005840 23.780 492 283 17 279 750 385 804 7.02e-19 92.4
MS.gene62524.t1 MTR_5g090090 21.480 554 328 18 243 772 617 1087 1.17e-18 92.0
MS.gene62524.t1 MTR_5g090090 21.480 554 328 18 243 772 617 1087 1.30e-18 92.0
MS.gene62524.t1 MTR_4g118720 25.000 360 208 10 420 751 1132 1457 5.06e-18 90.1
MS.gene62524.t1 MTR_1g105050 25.000 500 268 19 279 750 398 818 7.87e-18 89.4
MS.gene62524.t1 MTR_4g035100 26.131 398 240 14 421 773 1581 1969 9.30e-18 89.4
MS.gene62524.t1 MTR_4g035100 26.131 398 240 14 421 773 1581 1969 9.71e-18 89.4
MS.gene62524.t1 MTR_4g078495 36.170 141 84 2 630 770 538 672 6.98e-17 85.9
MS.gene62524.t1 MTR_4g078460 36.170 141 84 2 630 770 824 958 7.44e-17 86.3
MS.gene62524.t1 MTR_1g044120 35.897 156 93 3 608 762 981 1130 2.77e-16 84.3
MS.gene62524.t1 MTR_1g053955 31.481 162 105 2 606 767 1055 1210 8.68e-16 82.8
MS.gene62524.t1 MTR_7g045505 24.485 388 242 12 421 781 126 489 1.71e-15 81.3
MS.gene62524.t1 MTR_7g045505 24.485 388 242 12 421 781 126 489 2.48e-15 80.9
MS.gene62524.t1 MTR_7g089190 37.879 132 76 2 631 761 668 794 5.62e-15 79.7
MS.gene62524.t1 MTR_1g115215 30.769 143 93 2 631 773 887 1023 9.68e-14 75.9
MS.gene62524.t1 MTR_2g084630 34.559 136 84 2 631 762 868 1002 2.41e-12 71.2
MS.gene62524.t1 MTR_2g012830 31.081 148 94 3 627 773 1146 1286 2.48e-12 71.6
MS.gene62524.t1 MTR_4g049500 32.571 175 102 6 595 767 750 910 1.65e-11 68.6
MS.gene62524.t1 MTR_2g084630 32.836 134 80 3 631 762 874 999 3.19e-11 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62524.t1 AT1G05490 48.638 771 324 18 85 817 666 1402 0.0 655
MS.gene62524.t1 AT3G24340 46.100 718 342 13 106 816 448 1127 0.0 587
MS.gene62524.t1 AT3G42670 33.655 725 423 18 111 801 541 1241 7.83e-109 362
MS.gene62524.t1 AT3G42670 33.655 725 423 18 111 801 541 1241 7.83e-109 362
MS.gene62524.t1 AT3G42670 33.655 725 423 18 111 801 541 1241 7.83e-109 362
MS.gene62524.t1 AT3G42670 33.425 727 424 18 111 801 541 1243 2.82e-108 360
MS.gene62524.t1 AT5G20420 33.381 695 414 15 111 774 547 1223 2.18e-106 355
MS.gene62524.t1 AT2G16390 31.646 632 359 16 155 767 270 847 5.09e-88 298
MS.gene62524.t1 AT2G16390 31.646 632 359 16 155 767 273 850 5.37e-88 298
MS.gene62524.t1 AT2G21450 31.232 698 388 23 110 777 57 692 2.52e-87 292
MS.gene62524.t1 AT2G21450 31.541 688 379 23 110 767 148 773 8.79e-87 293
MS.gene62524.t1 AT2G21450 31.541 688 379 23 110 767 155 780 1.91e-86 292
MS.gene62524.t1 AT3G19210 24.694 571 332 16 239 770 176 687 2.31e-33 138
MS.gene62524.t1 AT3G19210 24.694 571 332 16 239 770 176 687 3.45e-33 138
MS.gene62524.t1 AT3G19210 24.253 569 335 17 239 770 176 685 1.32e-32 136
MS.gene62524.t1 AT2G18760 26.110 563 306 21 243 767 444 934 8.11e-29 124
MS.gene62524.t1 AT2G18760 26.110 563 306 21 243 767 385 875 8.68e-29 124
MS.gene62524.t1 AT2G18760 26.110 563 306 21 243 767 385 875 8.68e-29 124
MS.gene62524.t1 AT2G18760 26.110 563 306 21 243 767 385 875 8.68e-29 124
MS.gene62524.t1 AT2G18760 26.110 563 306 21 243 767 385 875 8.68e-29 124
MS.gene62524.t1 AT2G18760 26.110 563 306 21 243 767 385 875 8.68e-29 124
MS.gene62524.t1 AT2G18760 26.110 563 306 21 243 767 385 875 8.68e-29 124
MS.gene62524.t1 AT4G31900 22.924 602 357 19 218 794 199 718 4.33e-27 119
MS.gene62524.t1 AT4G31900 22.924 602 357 19 218 794 158 677 4.61e-27 119
MS.gene62524.t1 AT4G31900 22.924 602 357 19 218 794 199 718 6.36e-27 118
MS.gene62524.t1 AT5G63950 24.248 565 317 20 243 762 377 875 3.32e-25 113
MS.gene62524.t1 AT5G63950 25.166 453 252 15 368 762 435 858 2.29e-23 107
MS.gene62524.t1 AT5G44800 23.768 568 332 22 206 750 648 1137 3.75e-23 107
MS.gene62524.t1 AT2G25170 22.667 600 371 17 233 801 288 825 9.36e-23 105
MS.gene62524.t1 AT2G25170 22.667 600 371 17 233 801 262 799 1.03e-22 105
MS.gene62524.t1 AT2G25170 22.667 600 371 17 233 801 262 799 1.03e-22 105
MS.gene62524.t1 AT2G25170 22.667 600 371 17 233 801 279 816 1.13e-22 105
MS.gene62524.t1 AT1G08600 36.025 161 95 2 614 766 1116 1276 6.33e-21 99.4
MS.gene62524.t1 AT1G08600 36.025 161 95 2 614 766 1116 1276 6.33e-21 99.4
MS.gene62524.t1 AT1G08600 36.025 161 95 2 614 766 1116 1276 6.33e-21 99.4
MS.gene62524.t1 AT1G08600 36.025 161 95 2 614 766 1095 1255 7.40e-21 99.4
MS.gene62524.t1 AT2G13370 23.423 555 316 21 242 772 624 1093 1.74e-20 98.2
MS.gene62524.t1 AT2G13370 23.423 555 316 21 242 772 624 1093 1.74e-20 98.2
MS.gene62524.t1 AT2G13370 23.423 555 316 21 242 772 624 1093 1.74e-20 98.2
MS.gene62524.t1 AT5G66750 23.508 553 334 18 279 807 224 711 7.02e-20 95.5
MS.gene62524.t1 AT5G18620 23.197 513 300 14 279 774 216 651 3.06e-18 90.5
MS.gene62524.t1 AT5G18620 24.384 365 234 8 420 774 319 651 3.06e-18 90.5
MS.gene62524.t1 AT3G06400 23.720 371 229 8 420 774 314 646 6.85e-18 89.4
MS.gene62524.t1 AT3G06400 23.720 371 229 8 420 774 314 646 7.09e-18 89.4
MS.gene62524.t1 AT3G06400 23.720 371 229 8 420 774 314 646 7.22e-18 89.4
MS.gene62524.t1 AT5G19310 23.517 489 288 15 279 750 144 563 7.59e-18 89.0
MS.gene62524.t1 AT2G28290 24.863 366 201 10 420 751 881 1206 8.34e-18 89.7
MS.gene62524.t1 AT2G28290 24.863 366 201 10 420 751 882 1207 8.34e-18 89.7
MS.gene62524.t1 AT2G28290 24.863 366 201 10 420 751 881 1206 8.61e-18 89.7
MS.gene62524.t1 AT2G28290 24.863 366 201 10 420 751 881 1206 8.76e-18 89.7
MS.gene62524.t1 AT2G28290 24.863 366 201 10 420 751 881 1206 8.76e-18 89.7
MS.gene62524.t1 AT2G28290 24.863 366 201 10 420 751 882 1207 8.91e-18 89.4
MS.gene62524.t1 AT5G19310 23.517 489 288 15 279 750 408 827 1.10e-17 89.0
MS.gene62524.t1 AT1G03750 36.170 141 84 2 630 770 545 679 2.80e-17 87.4
MS.gene62524.t1 AT2G44980 25.840 387 217 13 421 777 181 527 4.80e-17 86.7
MS.gene62524.t1 AT5G43530 39.850 133 74 2 630 762 1128 1254 7.71e-17 86.3
MS.gene62524.t1 AT3G06010 24.432 352 211 11 420 750 528 845 1.25e-16 85.5
MS.gene62524.t1 AT2G44980 26.396 394 219 15 421 777 186 545 1.36e-16 85.1
MS.gene62524.t1 AT2G44980 26.396 394 219 15 421 777 187 546 1.45e-16 85.1
MS.gene62524.t1 AT3G12810 32.099 162 104 2 606 767 1064 1219 2.84e-16 84.3
MS.gene62524.t1 AT3G12810 32.099 162 104 2 606 767 1068 1223 3.09e-16 84.3
MS.gene62524.t1 AT5G05130 36.025 161 91 3 631 790 727 876 3.19e-16 84.0
MS.gene62524.t1 AT3G32330 23.018 391 199 12 237 605 17 327 3.19e-14 75.1
MS.gene62524.t1 AT1G11100 30.573 157 103 2 630 786 929 1079 5.58e-14 76.6
MS.gene62524.t1 AT1G11100 30.573 157 103 2 630 786 973 1123 6.39e-14 76.6
MS.gene62524.t1 AT1G11100 30.573 157 103 2 630 786 1072 1222 7.36e-14 76.6
MS.gene62524.t1 AT1G11100 30.573 157 103 2 630 786 1116 1266 7.90e-14 76.3
MS.gene62524.t1 AT1G11100 30.573 157 103 2 630 786 1115 1265 8.25e-14 76.3
MS.gene62524.t1 AT3G54280 31.973 147 93 3 629 773 1830 1971 2.70e-13 74.7
MS.gene62524.t1 AT3G54280 31.973 147 93 3 629 773 1861 2002 2.99e-13 74.7
MS.gene62524.t1 AT1G61140 31.915 141 88 3 630 769 966 1099 2.09e-12 71.6
MS.gene62524.t1 AT1G61140 31.915 141 88 3 630 769 1124 1257 2.10e-12 71.6
MS.gene62524.t1 AT3G54460 35.714 140 78 5 627 762 894 1025 2.80e-12 71.2
MS.gene62524.t1 AT3G54460 35.714 140 78 5 627 762 1191 1322 3.21e-12 71.2
MS.gene62524.t1 AT1G50410 30.137 146 90 3 631 773 828 964 4.17e-12 70.5
MS.gene62524.t1 AT1G50410 30.137 146 90 3 631 773 828 964 4.17e-12 70.5
MS.gene62524.t1 AT1G50410 30.137 146 90 3 631 773 828 964 4.17e-12 70.5
MS.gene62524.t1 AT3G20010 30.769 143 93 2 631 773 628 764 6.51e-12 69.7
MS.gene62524.t1 AT3G20010 30.769 143 93 2 631 773 603 739 6.55e-12 69.7
MS.gene62524.t1 AT3G20010 30.769 143 93 2 631 773 894 1030 1.16e-11 69.3
MS.gene62524.t1 AT3G20010 30.769 143 93 2 631 773 894 1030 1.16e-11 69.3
MS.gene62524.t1 AT3G20010 30.769 143 93 2 631 773 894 1030 1.16e-11 69.3
MS.gene62524.t1 AT5G22750 30.968 155 101 2 608 762 858 1006 1.99e-11 68.6
MS.gene62524.t1 AT1G02670 32.639 144 89 3 631 773 539 675 3.66e-11 67.4
MS.gene62524.t1 AT1G02670 32.639 144 89 3 631 773 512 648 3.91e-11 67.0
MS.gene62524.t1 AT1G02670 32.639 144 89 3 631 773 516 652 5.88e-11 66.6
MS.gene62524.t1 AT1G02670 32.639 144 89 3 631 773 527 663 6.10e-11 66.6
MS.gene62524.t1 AT1G05120 34.783 138 78 4 631 767 725 851 6.27e-11 66.6
MS.gene62524.t1 AT1G05120 34.783 138 78 4 631 767 682 808 7.33e-11 66.6

Find 171 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTACAGATTGGAAATTT+AGG 0.126449 7.3:-70275705 MS.gene62524:intron
ATATGACTTCTTTGATTTCT+TGG 0.141776 7.3:+70274498 None:intergenic
CTCCATTCCAGAACAATTTC+TGG 0.174401 7.3:-70274422 MS.gene62524:CDS
TATGGTCAGCCTTGTCTTTC+CGG 0.181588 7.3:+70274923 None:intergenic
AGCTTCTAGGGTTGTACTTC+TGG 0.196779 7.3:-70273665 MS.gene62524:CDS
GAAATCAAAGAAGTCATATT+TGG 0.244946 7.3:-70274494 MS.gene62524:CDS
ATTCCTTCTTCTTCTCAATA+TGG 0.245100 7.3:+70276196 None:intergenic
CGGAGTTGTGAACCTGTTTC+GGG 0.246918 7.3:-70274294 MS.gene62524:CDS
TTCAAAGAAACAAGCATTCT+TGG 0.249693 7.3:-70274660 MS.gene62524:CDS
AGCCTTGTCTTTCCGGTTCC+AGG 0.258151 7.3:+70274930 None:intergenic
AACAATAATCGGAAAGCATT+TGG 0.260267 7.3:+70274869 None:intergenic
ATGATTTATGTCCCTGCTTA+TGG 0.263420 7.3:+70274325 None:intergenic
TATAAAGTTTGTTTCAAATC+TGG 0.265369 7.3:+70275071 None:intergenic
GCGGAGTTGTGAACCTGTTT+CGG 0.280802 7.3:-70274295 MS.gene62524:CDS
ATTTAAAAGCAGAGGTTAAT+TGG 0.281211 7.3:+70273839 None:intergenic
AAGAAGGCTTTGAATTCATT+TGG 0.281224 7.3:-70275037 MS.gene62524:CDS
TGCTCCTTGCGCAATGCTAT+TGG 0.289113 7.3:-70276113 MS.gene62524:CDS
ATCTACAGATTGGAAATTTA+GGG 0.298187 7.3:-70275704 MS.gene62524:intron
AAAATAAGCTTCCTGGGTTA+AGG 0.301129 7.3:-70274179 MS.gene62524:CDS
TTAGCTTAGTTGGAGCTTCT+AGG 0.302332 7.3:-70273678 MS.gene62524:CDS
TTTGTTTCAAATCTGGAATT+AGG 0.316184 7.3:+70275078 None:intergenic
CGAAGTGTTATGGGATGAAT+TGG 0.316690 7.3:-70275923 MS.gene62524:CDS
AGGAGGAGAACGAAGTGTTA+TGG 0.324265 7.3:-70275933 MS.gene62524:CDS
CTTGTTGTGTTGCCCGAAAC+AGG 0.324806 7.3:+70274282 None:intergenic
TTAAGCAATCCCGAGTTATC+TGG 0.334660 7.3:-70274774 MS.gene62524:CDS
CAGAAGCGAATCATCCTTTC+CGG 0.338463 7.3:-70274447 MS.gene62524:CDS
GGAATCAGCTACAGTTTGTT+TGG 0.342280 7.3:-70274639 MS.gene62524:CDS
ATCCTTGAAAATAAGCTTCC+TGG 0.344675 7.3:-70274186 MS.gene62524:CDS
TCCTGGGTTAAGGGACTGTT+TGG 0.348954 7.3:-70274169 MS.gene62524:CDS
CACAGTTGAATCATTCTTCA+TGG 0.355596 7.3:+70276077 None:intergenic
GGAGGAGAACGAAGTGTTAT+GGG 0.356347 7.3:-70275932 MS.gene62524:CDS
CTTGCAATGAAGGCACAGTT+TGG 0.359027 7.3:-70275103 MS.gene62524:CDS
ATTGTAAAGGCGCATGGAAT+TGG 0.364774 7.3:+70273872 None:intergenic
ACTAATTTCTCTTGCAATGA+AGG 0.366544 7.3:-70275113 MS.gene62524:CDS
AAATAAGCTTCCTGGGTTAA+GGG 0.369051 7.3:-70274178 MS.gene62524:CDS
TCTGAGGGAATTAGCTTAGT+TGG 0.375316 7.3:-70273688 MS.gene62524:CDS
GGGAGGATGAATTCAAGAAA+TGG 0.377896 7.3:-70274821 MS.gene62524:CDS
AGATTTCCCTGTGAAGGTTC+AGG 0.380692 7.3:-70275215 MS.gene62524:CDS
CTGCTAGTATACTACTAACT+TGG 0.381021 7.3:-70274842 MS.gene62524:CDS
TCTGCTCGCTGAAGGGACTA+TGG 0.381344 7.3:-70273557 MS.gene62524:CDS
CAAGAAGCTGAAATTGCTAA+TGG 0.382622 7.3:-70274238 MS.gene62524:CDS
GCTCCTTGCGCAATGCTATT+GGG 0.383553 7.3:-70276112 MS.gene62524:CDS
CTTTCTTCCTGAACCTTCAC+AGG 0.384915 7.3:+70275208 None:intergenic
AGCTCAGGCGGTATCGTGTT+AGG 0.387985 7.3:+70274360 None:intergenic
ATTACGGATTGCTTGACCTT+TGG 0.391840 7.3:+70273772 None:intergenic
ATTTCTCATGCTCCTGGAAC+CGG 0.395216 7.3:-70274942 MS.gene62524:CDS
CTTGGACTCATCGTCATTAT+CGG 0.401265 7.3:+70273462 None:intergenic
ACGGATTGCTTGACCTTTGG+AGG 0.405791 7.3:+70273775 None:intergenic
AAAGCTCCCAGAAATTGTTC+TGG 0.406710 7.3:+70274415 None:intergenic
GGAAGAAAGGCATCATTTGA+TGG 0.407266 7.3:-70275194 MS.gene62524:CDS
CTAGCAGGAGCAACAATAAT+CGG 0.410720 7.3:+70274858 None:intergenic
ACAATAATCGGAAAGCATTT+GGG 0.412501 7.3:+70274870 None:intergenic
AAAATTCATATCACAGCTCT+TGG 0.412571 7.3:+70273444 None:intergenic
ATTGTTCTGGAATGGAGTTC+CGG 0.412671 7.3:+70274428 None:intergenic
TTTCCTTGAATGTTCTCTTT+CGG 0.413344 7.3:-70274031 MS.gene62524:CDS
CTTCCGAAAGAGAACATTCA+AGG 0.414255 7.3:+70274028 None:intergenic
TTCAACTGTGTTCGAGTTCA+AGG 0.414802 7.3:-70276064 MS.gene62524:CDS
ATCGTTGAAGCTATGGATTA+CGG 0.422848 7.3:+70273756 None:intergenic
ACTGAGGAAGGAGCGAGATA+TGG 0.423086 7.3:-70273353 MS.gene62524:CDS
TGCTCTCGACAAGGATCAGC+TGG 0.426412 7.3:-70273995 MS.gene62524:CDS
TGCATTATTTCTCATGCTCC+TGG 0.431774 7.3:-70274948 MS.gene62524:CDS
CCTTTGTTCATGTGCACAAA+GGG 0.432796 7.3:-70274212 MS.gene62524:CDS
AACAAAGGAAGGGATTATAA+AGG 0.438543 7.3:-70276231 MS.gene62524:CDS
TTGTACTTCTGGATGTTGTA+TGG 0.448145 7.3:-70273654 MS.gene62524:CDS
ATATGGTAAACAGGCTGAAA+AGG 0.451018 7.3:-70273524 MS.gene62524:CDS
TGGTCTTTAATTAATTGTAA+AGG 0.452462 7.3:+70273859 None:intergenic
AAGGAAGTGTTGTTCATGTC+TGG 0.458301 7.3:-70273805 MS.gene62524:CDS
TCCATTCCAGAACAATTTCT+GGG 0.462331 7.3:-70274421 MS.gene62524:CDS
AAGCTATCAGCCGAGCGTAT+AGG 0.462639 7.3:-70273612 MS.gene62524:CDS
TCGTGTTCACATCGCGATGA+TGG 0.467848 7.3:+70275648 None:intergenic
TTGCAATGAAGGCACAGTTT+GGG 0.468703 7.3:-70275102 MS.gene62524:CDS
GTAAATGTTTCACTGTTTGA+TGG 0.471326 7.3:-70275170 MS.gene62524:CDS
TAGCTTAGTTGGAGCTTCTA+GGG 0.471979 7.3:-70273677 MS.gene62524:CDS
TTCTTAAGGTTCCATATCGA+TGG 0.475228 7.3:+70275985 None:intergenic
GAAAATGTGAAGGGGAGAAA+AGG 0.475800 7.3:-70274588 MS.gene62524:CDS
GCAAAAGACCATGGAGGAGG+AGG 0.480692 7.3:-70275950 MS.gene62524:CDS
ATAGCTTGTCTTTCGACTGA+AGG 0.494216 7.3:+70273628 None:intergenic
GGAGGATGAATTCAAGAAAT+GGG 0.494983 7.3:-70274820 MS.gene62524:CDS
AGAACGCTTCACTAAGCTTA+AGG 0.496015 7.3:+70274386 None:intergenic
CTGTGAAGGTTCAGGAAGAA+AGG 0.497585 7.3:-70275207 MS.gene62524:CDS
CAAGTTAGTAGTATACTAGC+AGG 0.498458 7.3:+70274843 None:intergenic
TTCGTTCTCCTCCTCCTCCA+TGG 0.498689 7.3:+70275942 None:intergenic
GGTTTGTTAGTTCTAGATGA+AGG 0.499262 7.3:-70274528 MS.gene62524:CDS
AATTTGCATCGTTGAAGCTA+TGG 0.500809 7.3:+70273749 None:intergenic
CTCAGACCATTTAAAAGCAG+AGG 0.505543 7.3:+70273831 None:intergenic
TCCTTGAAAATAAGCTTCCT+GGG 0.507367 7.3:-70274185 MS.gene62524:CDS
TCTTAAGGTTCCATATCGAT+GGG 0.513042 7.3:+70275986 None:intergenic
GGATTGCTTAAATTGTGAAA+CGG 0.514265 7.3:+70274786 None:intergenic
GGAATCAAGTAAACAGAGAA+TGG 0.514605 7.3:-70276259 MS.gene62524:CDS
ATATGCCTTGAGAAACACTA+TGG 0.514676 7.3:+70274905 None:intergenic
TTCTTGATCTTATCATCAGA+AGG 0.516557 7.3:+70274255 None:intergenic
CCTTTGTGCACATGAACAAA+GGG 0.519201 7.3:+70274213 None:intergenic
GCCACCTTCACTGCAAGGAT+CGG 0.519684 7.3:+70274971 None:intergenic
CATCCATATTGAGAAGAAGA+AGG 0.520459 7.3:-70276199 MS.gene62524:CDS
AAGATTCTGCTAAAAGCTCC+TGG 0.520895 7.3:-70274549 MS.gene62524:CDS
CGCTGAAGGGACTATGGAGG+AGG 0.528705 7.3:-70273551 MS.gene62524:CDS
GGACTGTTTGGTGACTTTGA+AGG 0.530361 7.3:-70274157 MS.gene62524:CDS
GTTGATAGATTTCCCTGTGA+AGG 0.531771 7.3:-70275221 MS.gene62524:CDS
TAATTAATTGTAAAGGCGCA+TGG 0.533525 7.3:+70273866 None:intergenic
CCCTTTGTTCATGTGCACAA+AGG 0.533803 7.3:-70274213 MS.gene62524:CDS
GTCTCCACAGAAACCGGTGG+TGG 0.533923 7.3:+70276012 None:intergenic
CCCTTTGTGCACATGAACAA+AGG 0.536847 7.3:+70274212 None:intergenic
CAAATATATCTCACCACTAG+TGG 0.541968 7.3:-70275562 MS.gene62524:intron
ACAGCCACCACCGGTTTCTG+TGG 0.546294 7.3:-70276016 MS.gene62524:CDS
ATCAGCCGAGCGTATAGGAT+TGG 0.547339 7.3:-70273607 MS.gene62524:CDS
GAATGGTGGAGAACAAAGGA+AGG 0.552165 7.3:-70276242 MS.gene62524:CDS
ATGCGAATAGAACCTTTGCT+TGG 0.557067 7.3:+70273725 None:intergenic
CTTAGACTGAACCCATATGA+AGG 0.561386 7.3:-70273967 MS.gene62524:CDS
AATGCCGATCCTTGCAGTGA+AGG 0.561759 7.3:-70274975 MS.gene62524:CDS
TAGTCCCTTCAGCGAGCAGA+TGG 0.563533 7.3:+70273560 None:intergenic
ATGTTCTTTACCAGATAACT+CGG 0.563939 7.3:+70274764 None:intergenic
TCCCAGAAATTGTTCTGGAA+TGG 0.564172 7.3:+70274420 None:intergenic
TTATATCGTCATCAACAAGA+AGG 0.567121 7.3:-70275053 MS.gene62524:CDS
TCTGACCAATCCTATACGCT+CGG 0.568050 7.3:+70273602 None:intergenic
ATTCAAGGAAAAGCGATGGA+TGG 0.568688 7.3:+70274043 None:intergenic
CATACCATCTGCTCGCTGAA+GGG 0.572458 7.3:-70273564 MS.gene62524:CDS
TCTACAAAAGCTTGTTCTGA+GGG 0.573558 7.3:-70273703 MS.gene62524:CDS
AGAATCTGACGCTGTATCCA+TGG 0.574662 7.3:-70275887 MS.gene62524:intron
AAGGAAAAGCGATGGATGGA+CGG 0.575965 7.3:+70274047 None:intergenic
GAACATTCAAGGAAAAGCGA+TGG 0.581694 7.3:+70274039 None:intergenic
AATCGGAAAGCATTTGGGAA+AGG 0.582011 7.3:+70274875 None:intergenic
CGATGCAAATTGCCAAGCAA+AGG 0.584100 7.3:-70273737 MS.gene62524:CDS
ATCGATATGGAACCTTAAGA+AGG 0.586361 7.3:-70275983 MS.gene62524:CDS
AAGGAATCTATTGATAACAA+TGG 0.588038 7.3:-70276180 MS.gene62524:CDS
TCAAAGAAGTCATATTTGGA+AGG 0.589347 7.3:-70274490 MS.gene62524:CDS
ACATACCATCTGCTCGCTGA+AGG 0.589622 7.3:-70273565 MS.gene62524:CDS
TGCTAGTATACTACTAACTT+GGG 0.589951 7.3:-70274841 MS.gene62524:CDS
ATGCAGCCACCTTCACTGCA+AGG 0.591972 7.3:+70274966 None:intergenic
TGTTTGGTGACTTTGAAGGC+AGG 0.596768 7.3:-70274153 MS.gene62524:CDS
TAAGTGCGTGGTACTGAGGA+AGG 0.597578 7.3:-70273365 MS.gene62524:CDS
ATCTACAAAAGCTTGTTCTG+AGG 0.600049 7.3:-70273704 MS.gene62524:CDS
GTAAACAGGCTGAAAAGGAC+CGG 0.601881 7.3:-70273519 MS.gene62524:CDS
TCTTGATCTTATCATCAGAA+GGG 0.604374 7.3:+70274256 None:intergenic
AATGGTGGAGAACAAAGGAA+GGG 0.609814 7.3:-70276241 MS.gene62524:CDS
TTTCTTCCTGAACCTTCACA+GGG 0.609885 7.3:+70275209 None:intergenic
TGTTCTTTACCAGATAACTC+GGG 0.610730 7.3:+70274765 None:intergenic
CAGAGAATGGTGGAGAACAA+AGG 0.613980 7.3:-70276246 MS.gene62524:CDS
GATATGGAACCTTAAGAAGG+TGG 0.615242 7.3:-70275980 MS.gene62524:CDS
TCTGGAATGGAGTTCCGGAA+AGG 0.615617 7.3:+70274433 None:intergenic
ACAAGCTACACAGCCACCAC+CGG 0.619069 7.3:-70276025 MS.gene62524:CDS
GCCGATCCTTGCAGTGAAGG+TGG 0.620537 7.3:-70274972 MS.gene62524:CDS
GTGTTCGAGTTCAAGGAGGA+TGG 0.621068 7.3:-70276057 MS.gene62524:CDS
GGTGTATATTGCAAATTGTG+TGG 0.625204 7.3:-70275603 MS.gene62524:CDS
CTTAAGGTTCCATATCGATG+GGG 0.625433 7.3:+70275987 None:intergenic
AGTGCAAAAGACCATGGAGG+AGG 0.626765 7.3:-70275953 MS.gene62524:CDS
AGACATGTTTGTTAAGTGCG+TGG 0.627760 7.3:-70273377 MS.gene62524:CDS
ATCAAGTAAACAGAGAATGG+TGG 0.632548 7.3:-70276256 MS.gene62524:CDS
TGTCCCAATAGCATTGCGCA+AGG 0.633073 7.3:+70276109 None:intergenic
AAAAGCATGAAAATGTGAAG+GGG 0.633486 7.3:-70274596 MS.gene62524:CDS
GCTGACCATAGTGTTTCTCA+AGG 0.634254 7.3:-70274910 MS.gene62524:CDS
TTAAGGTTCCATATCGATGG+GGG 0.634311 7.3:+70275988 None:intergenic
AGAAGAATCTGCTCTCGACA+AGG 0.638283 7.3:-70274004 MS.gene62524:CDS
GAAACCGTGCTTACCACTAG+TGG 0.644506 7.3:+70275549 None:intergenic
ATATTGCAAATTGTGTGGTG+TGG 0.649175 7.3:-70275598 MS.gene62524:CDS
GAATTCAAGAAATGGGACAT+CGG 0.650153 7.3:-70274813 MS.gene62524:CDS
CTCCTGGAACCGGAAAGACA+AGG 0.656255 7.3:-70274932 MS.gene62524:CDS
TCATCTAGAACTAACAAACC+AGG 0.659449 7.3:+70274531 None:intergenic
GTCTGGCAAAGATCCTCCAA+AGG 0.659751 7.3:-70273788 MS.gene62524:CDS
CTTTGTTCATGTGCACAAAG+GGG 0.661128 7.3:-70274211 MS.gene62524:CDS
AACAAAGCAAGAAATGATGG+AGG 0.661151 7.3:-70276280 None:intergenic
ACCAAACAGTCCCTTAACCC+AGG 0.663907 7.3:+70274168 None:intergenic
TAGTATACTACTAACTTGGG+AGG 0.670120 7.3:-70274838 MS.gene62524:CDS
GCTCGCTGAAGGGACTATGG+AGG 0.670171 7.3:-70273554 MS.gene62524:CDS
GGTGTCTCCACAGAAACCGG+TGG 0.680014 7.3:+70276009 None:intergenic
TAGGATTGGTCAGAAGAGAG+TGG 0.680264 7.3:-70273593 MS.gene62524:CDS
AACTGTGTTCGAGTTCAAGG+AGG 0.688944 7.3:-70276061 MS.gene62524:CDS
TTGTTAAGTGCGTGGTACTG+AGG 0.691138 7.3:-70273369 MS.gene62524:CDS
GGACTCATCGTCATTATCGG+CGG 0.691472 7.3:+70273465 None:intergenic
TGGTAAAGAACATGCTGATG+CGG 0.695876 7.3:-70274754 MS.gene62524:CDS
AGCATAATATTAATGAAACG+CGG 0.743459 7.3:-70274704 MS.gene62524:CDS
AAAAGTGCAAAAGACCATGG+AGG 0.766977 7.3:-70275956 MS.gene62524:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGATTTATGTAAAATAACT+TGG - chr7.3:70274092-70274111 MS.gene62524:CDS 15.0%
!!! ATTTTAATTTTGTGAACTAT+AGG - chr7.3:70273790-70273809 MS.gene62524:CDS 15.0%
!! CTGTTTATATGTTTAAACTT+CGG - chr7.3:70273849-70273868 MS.gene62524:CDS 20.0%
!! GAAGCTACAAAAATAAAAAA+CGG - chr7.3:70273940-70273959 MS.gene62524:CDS 20.0%
!! TGGTCTTTAATTAATTGTAA+AGG + chr7.3:70275759-70275778 None:intergenic 20.0%
!! TTATCAACATATATATCACA+AGG + chr7.3:70274332-70274351 None:intergenic 20.0%
!!! AAACTTAAATGGTCTTAAAA+TGG + chr7.3:70273761-70273780 None:intergenic 20.0%
!!! TATAAAGTTTGTTTCAAATC+TGG + chr7.3:70274547-70274566 None:intergenic 20.0%
! AGCATAATATTAATGAAACG+CGG - chr7.3:70274911-70274930 MS.gene62524:CDS 25.0%
! ATCTACAGATTGGAAATTTA+GGG - chr7.3:70273911-70273930 MS.gene62524:CDS 25.0%
! ATGCATAGTTCAAACTTAAA+TGG + chr7.3:70273772-70273791 None:intergenic 25.0%
! ATTTAAAAGCAGAGGTTAAT+TGG + chr7.3:70275779-70275798 None:intergenic 25.0%
! GAAATCAAAGAAGTCATATT+TGG - chr7.3:70275121-70275140 MS.gene62524:CDS 25.0%
! GACATAAATCATCTAAAAAG+CGG - chr7.3:70275301-70275320 MS.gene62524:intron 25.0%
! TATCTACAGATTGGAAATTT+AGG - chr7.3:70273910-70273929 MS.gene62524:CDS 25.0%
! TGAATTCATTTGGAAAAACT+TGG - chr7.3:70274588-70274607 MS.gene62524:CDS 25.0%
!! AAAAAGCATGAAAATGTGAA+GGG - chr7.3:70275018-70275037 MS.gene62524:CDS 25.0%
!! AAGGAATCTATTGATAACAA+TGG - chr7.3:70273435-70273454 MS.gene62524:CDS 25.0%
!! ATATGACTTCTTTGATTTCT+TGG + chr7.3:70275120-70275139 None:intergenic 25.0%
!! GTGATATATATGTTGATAAG+AGG - chr7.3:70274333-70274352 MS.gene62524:CDS 25.0%
!! TTTGTTTCAAATCTGGAATT+AGG + chr7.3:70274540-70274559 None:intergenic 25.0%
!!! AATTAACCTCTGCTTTTAAA+TGG - chr7.3:70275778-70275797 MS.gene62524:intron 25.0%
!!! AATTTTTGTTCGAGTTTGTT+CGG - chr7.3:70275682-70275701 MS.gene62524:CDS 25.0%
!!! TTTCATGCTTTTTCTTATCT+TGG + chr7.3:70275011-70275030 None:intergenic 25.0%
AAAAAACAAGCTCAGATAAC+CGG + chr7.3:70276118-70276137 None:intergenic 30.0%
AAAATTCATATCACAGCTCT+TGG + chr7.3:70276174-70276193 None:intergenic 30.0%
AACAAAGGAAGGGATTATAA+AGG - chr7.3:70273384-70273403 MS.gene62524:CDS 30.0%
ACTAATTTCTCTTGCAATGA+AGG - chr7.3:70274502-70274521 MS.gene62524:CDS 30.0%
ATGTTCTTTACCAGATAACT+CGG + chr7.3:70274854-70274873 None:intergenic 30.0%
ATTCCTTCTTCTTCTCAATA+TGG + chr7.3:70273422-70273441 None:intergenic 30.0%
GGATTGCTTAAATTGTGAAA+CGG + chr7.3:70274832-70274851 None:intergenic 30.0%
GTAAATGTTTCACTGTTTGA+TGG - chr7.3:70274445-70274464 MS.gene62524:CDS 30.0%
TCAAAGAAGTCATATTTGGA+AGG - chr7.3:70275125-70275144 MS.gene62524:CDS 30.0%
TCTTGATCTTATCATCAGAA+GGG + chr7.3:70275362-70275381 None:intergenic 30.0%
TGCTAGTATACTACTAACTT+GGG - chr7.3:70274774-70274793 MS.gene62524:CDS 30.0%
TTATATCGTCATCAACAAGA+AGG - chr7.3:70274562-70274581 MS.gene62524:CDS 30.0%
TTCAAAGAAACAAGCATTCT+TGG - chr7.3:70274955-70274974 MS.gene62524:CDS 30.0%
TTCTTGATCTTATCATCAGA+AGG + chr7.3:70275363-70275382 None:intergenic 30.0%
TTTCCTTGAATGTTCTCTTT+CGG - chr7.3:70275584-70275603 MS.gene62524:CDS 30.0%
! AAAAAGTTTTTGCCATAAGC+AGG - chr7.3:70275278-70275297 MS.gene62524:intron 30.0%
! AAAAGCATGAAAATGTGAAG+GGG - chr7.3:70275019-70275038 MS.gene62524:CDS 30.0%
! AAAAGTTTTTGCCATAAGCA+GGG - chr7.3:70275279-70275298 MS.gene62524:intron 30.0%
! CTTAAATGGTCTTAAAATGG+AGG + chr7.3:70273758-70273777 None:intergenic 30.0%
! GAAAAAGCATGAAAATGTGA+AGG - chr7.3:70275017-70275036 MS.gene62524:CDS 30.0%
! TAATTAATTGTAAAGGCGCA+TGG + chr7.3:70275752-70275771 None:intergenic 30.0%
!! AACAATAATCGGAAAGCATT+TGG + chr7.3:70274749-70274768 None:intergenic 30.0%
!! AAGAAGGCTTTGAATTCATT+TGG - chr7.3:70274578-70274597 MS.gene62524:CDS 30.0%
!! ACAATAATCGGAAAGCATTT+GGG + chr7.3:70274748-70274767 None:intergenic 30.0%
!! TCAAAAGCACACATTTTATG+CGG - chr7.3:70273472-70273491 MS.gene62524:CDS 30.0%
!!! CTTGGTTACTTTTCTGATTT+TGG - chr7.3:70274110-70274129 MS.gene62524:CDS 30.0%
!!! TTGTTTTGACACCTTCATAT+GGG + chr7.3:70275662-70275681 None:intergenic 30.0%
!!! TTTGTTTTGACACCTTCATA+TGG + chr7.3:70275663-70275682 None:intergenic 30.0%
AAAATAAGCTTCCTGGGTTA+AGG - chr7.3:70275436-70275455 MS.gene62524:intron 35.0%
AAATAAGCTTCCTGGGTTAA+GGG - chr7.3:70275437-70275456 MS.gene62524:intron 35.0%
AATTTGCATCGTTGAAGCTA+TGG + chr7.3:70275869-70275888 None:intergenic 35.0%
ACTATGGAGGAGGAAAAATA+TGG - chr7.3:70276074-70276093 MS.gene62524:CDS 35.0%
ATATGCCTTGAGAAACACTA+TGG + chr7.3:70274713-70274732 None:intergenic 35.0%
ATATGGTAAACAGGCTGAAA+AGG - chr7.3:70276091-70276110 MS.gene62524:CDS 35.0%
ATATTGCAAATTGTGTGGTG+TGG - chr7.3:70274017-70274036 MS.gene62524:CDS 35.0%
ATCAAGTAAACAGAGAATGG+TGG - chr7.3:70273359-70273378 MS.gene62524:CDS 35.0%
ATCCTTGAAAATAAGCTTCC+TGG - chr7.3:70275429-70275448 MS.gene62524:intron 35.0%
ATCGATATGGAACCTTAAGA+AGG - chr7.3:70273632-70273651 MS.gene62524:CDS 35.0%
ATGATTTATGTCCCTGCTTA+TGG + chr7.3:70275293-70275312 None:intergenic 35.0%
CAAATATATCTCACCACTAG+TGG - chr7.3:70274053-70274072 MS.gene62524:CDS 35.0%
CAAGAAGCTGAAATTGCTAA+TGG - chr7.3:70275377-70275396 MS.gene62524:intron 35.0%
CAAGTTAGTAGTATACTAGC+AGG + chr7.3:70274775-70274794 None:intergenic 35.0%
CACAGTTGAATCATTCTTCA+TGG + chr7.3:70273541-70273560 None:intergenic 35.0%
CATCCATATTGAGAAGAAGA+AGG - chr7.3:70273416-70273435 MS.gene62524:CDS 35.0%
CTGCTAGTATACTACTAACT+TGG - chr7.3:70274773-70274792 MS.gene62524:CDS 35.0%
CTTAAAATGGAGGATTACCA+TGG + chr7.3:70273748-70273767 None:intergenic 35.0%
GAATTCAAGAAATGGGACAT+CGG - chr7.3:70274802-70274821 MS.gene62524:CDS 35.0%
GGAATCAAGTAAACAGAGAA+TGG - chr7.3:70273356-70273375 MS.gene62524:CDS 35.0%
GGAGGAAAAATATGGTAAAC+AGG - chr7.3:70276082-70276101 MS.gene62524:CDS 35.0%
GGAGGATGAATTCAAGAAAT+GGG - chr7.3:70274795-70274814 MS.gene62524:CDS 35.0%
GGATGACAAAAATGATTTCG+TGG - chr7.3:70274932-70274951 MS.gene62524:CDS 35.0%
GGTGTATATTGCAAATTGTG+TGG - chr7.3:70274012-70274031 MS.gene62524:CDS 35.0%
TAGTATACTACTAACTTGGG+AGG - chr7.3:70274777-70274796 MS.gene62524:CDS 35.0%
TCCATTCCAGAACAATTTCT+GGG - chr7.3:70275194-70275213 MS.gene62524:CDS 35.0%
TCCTTGAAAATAAGCTTCCT+GGG - chr7.3:70275430-70275449 MS.gene62524:intron 35.0%
TCTTAAGGTTCCATATCGAT+GGG + chr7.3:70273632-70273651 None:intergenic 35.0%
TGTTCTTTACCAGATAACTC+GGG + chr7.3:70274853-70274872 None:intergenic 35.0%
TTCATTTGGAAAAACTTGGC+AGG - chr7.3:70274592-70274611 MS.gene62524:CDS 35.0%
TTCTTAAGGTTCCATATCGA+TGG + chr7.3:70273633-70273652 None:intergenic 35.0%
TTGTACTTCTGGATGTTGTA+TGG - chr7.3:70275961-70275980 MS.gene62524:CDS 35.0%
! ATCGTTGAAGCTATGGATTA+CGG + chr7.3:70275862-70275881 None:intergenic 35.0%
! ATCTACAAAAGCTTGTTCTG+AGG - chr7.3:70275911-70275930 MS.gene62524:CDS 35.0%
! GTGTGTTACTATCTACAGAT+TGG - chr7.3:70273901-70273920 MS.gene62524:CDS 35.0%
! TCATCTAGAACTAACAAACC+AGG + chr7.3:70275087-70275106 None:intergenic 35.0%
! TCTACAAAAGCTTGTTCTGA+GGG - chr7.3:70275912-70275931 MS.gene62524:CDS 35.0%
! TTTTAAATGGTCTGAGGGAA+AGG - chr7.3:70275791-70275810 MS.gene62524:intron 35.0%
!! GGTTTGTTAGTTCTAGATGA+AGG - chr7.3:70275087-70275106 MS.gene62524:CDS 35.0%
!! TCTGCTTTTAAATGGTCTGA+GGG - chr7.3:70275786-70275805 MS.gene62524:intron 35.0%
!! TGCAAAGTATTGTGTACTTG+CGG - chr7.3:70274189-70274208 MS.gene62524:CDS 35.0%
!!! CAAAAACTTTTTAGCTCAGG+CGG + chr7.3:70275270-70275289 None:intergenic 35.0%
!!! TGGCAAAAACTTTTTAGCTC+AGG + chr7.3:70275273-70275292 None:intergenic 35.0%
AAAGCTCCCAGAAATTGTTC+TGG + chr7.3:70275203-70275222 None:intergenic 40.0%
AAGATTCTGCTAAAAGCTCC+TGG - chr7.3:70275066-70275085 MS.gene62524:CDS 40.0%
AGAACGCTTCACTAAGCTTA+AGG + chr7.3:70275232-70275251 None:intergenic 40.0%
ATGCGAATAGAACCTTTGCT+TGG + chr7.3:70275893-70275912 None:intergenic 40.0%
ATTCAAGGAAAAGCGATGGA+TGG + chr7.3:70275575-70275594 None:intergenic 40.0%
CCTTTGTGCACATGAACAAA+GGG + chr7.3:70275405-70275424 None:intergenic 40.0%
CCTTTGTTCATGTGCACAAA+GGG - chr7.3:70275403-70275422 MS.gene62524:intron 40.0%
CTAGCAGGAGCAACAATAAT+CGG + chr7.3:70274760-70274779 None:intergenic 40.0%
CTCAGACCATTTAAAAGCAG+AGG + chr7.3:70275787-70275806 None:intergenic 40.0%
CTCCATTCCAGAACAATTTC+TGG - chr7.3:70275193-70275212 MS.gene62524:CDS 40.0%
CTTAAGGTTCCATATCGATG+GGG + chr7.3:70273631-70273650 None:intergenic 40.0%
CTTAGACTGAACCCATATGA+AGG - chr7.3:70275648-70275667 MS.gene62524:CDS 40.0%
CTTCCGAAAGAGAACATTCA+AGG + chr7.3:70275590-70275609 None:intergenic 40.0%
CTTGGACTCATCGTCATTAT+CGG + chr7.3:70276156-70276175 None:intergenic 40.0%
CTTTGTTCATGTGCACAAAG+GGG - chr7.3:70275404-70275423 MS.gene62524:intron 40.0%
GAAAATGTGAAGGGGAGAAA+AGG - chr7.3:70275027-70275046 MS.gene62524:CDS 40.0%
GAACATTCAAGGAAAAGCGA+TGG + chr7.3:70275579-70275598 None:intergenic 40.0%
GATATGGAACCTTAAGAAGG+TGG - chr7.3:70273635-70273654 MS.gene62524:CDS 40.0%
GGGAGGATGAATTCAAGAAA+TGG - chr7.3:70274794-70274813 MS.gene62524:CDS 40.0%
TAGCTTAGTTGGAGCTTCTA+GGG - chr7.3:70275938-70275957 MS.gene62524:CDS 40.0%
TGGTAAAGAACATGCTGATG+CGG - chr7.3:70274861-70274880 MS.gene62524:CDS 40.0%
TTAAGCAATCCCGAGTTATC+TGG - chr7.3:70274841-70274860 MS.gene62524:CDS 40.0%
TTAAGGTTCCATATCGATGG+GGG + chr7.3:70273630-70273649 None:intergenic 40.0%
TTAGCTTAGTTGGAGCTTCT+AGG - chr7.3:70275937-70275956 MS.gene62524:CDS 40.0%
TTTCTTCCTGAACCTTCACA+GGG + chr7.3:70274409-70274428 None:intergenic 40.0%
! AAAAGTGCAAAAGACCATGG+AGG - chr7.3:70273659-70273678 MS.gene62524:CDS 40.0%
! AATGGTGGAGAACAAAGGAA+GGG - chr7.3:70273374-70273393 MS.gene62524:CDS 40.0%
! ACTTTTCTCGACGAAGAGAT+TGG - chr7.3:70273991-70274010 MS.gene62524:CDS 40.0%
! AGACATGTTTGTTAAGTGCG+TGG - chr7.3:70276238-70276257 MS.gene62524:CDS 40.0%
! ATAGCTTGTCTTTCGACTGA+AGG + chr7.3:70275990-70276009 None:intergenic 40.0%
! ATTACGGATTGCTTGACCTT+TGG + chr7.3:70275846-70275865 None:intergenic 40.0%
! ATTGTAAAGGCGCATGGAAT+TGG + chr7.3:70275746-70275765 None:intergenic 40.0%
! CGAAGTGTTATGGGATGAAT+TGG - chr7.3:70273692-70273711 MS.gene62524:CDS 40.0%
! GGAAAAAGTGCAAAAGACCA+TGG - chr7.3:70273656-70273675 MS.gene62524:CDS 40.0%
! GGAATCAGCTACAGTTTGTT+TGG - chr7.3:70274976-70274995 MS.gene62524:CDS 40.0%
! GTTGATAGATTTCCCTGTGA+AGG - chr7.3:70274394-70274413 MS.gene62524:CDS 40.0%
! TCTGAGGGAATTAGCTTAGT+TGG - chr7.3:70275927-70275946 MS.gene62524:CDS 40.0%
! TGCATTATTTCTCATGCTCC+TGG - chr7.3:70274667-70274686 MS.gene62524:CDS 40.0%
! TTCAACTGTGTTCGAGTTCA+AGG - chr7.3:70273551-70273570 MS.gene62524:CDS 40.0%
! TTGCAATGAAGGCACAGTTT+GGG - chr7.3:70274513-70274532 MS.gene62524:CDS 40.0%
!! AAGGAAGTGTTGTTCATGTC+TGG - chr7.3:70275810-70275829 MS.gene62524:intron 40.0%
!! AATCGGAAAGCATTTGGGAA+AGG + chr7.3:70274743-70274762 None:intergenic 40.0%
!! ACCCAGGAAGCTTATTTTCA+AGG + chr7.3:70275434-70275453 None:intergenic 40.0%
!! ATTGTTCTGGAATGGAGTTC+CGG + chr7.3:70275190-70275209 None:intergenic 40.0%
!! CTCTGCTTTTAAATGGTCTG+AGG - chr7.3:70275785-70275804 MS.gene62524:intron 40.0%
!! GGAAGAAAGGCATCATTTGA+TGG - chr7.3:70274421-70274440 MS.gene62524:CDS 40.0%
!! TCCCAGAAATTGTTCTGGAA+TGG + chr7.3:70275198-70275217 None:intergenic 40.0%
!!! ACTTGTTTTACACGTGACAC+AGG - chr7.3:70274372-70274391 MS.gene62524:CDS 40.0%
!!! GCACTTTTTCCACCTTCTTA+AGG + chr7.3:70273647-70273666 None:intergenic 40.0%
AAGGAAAAGCGATGGATGGA+CGG + chr7.3:70275571-70275590 None:intergenic 45.0%
AGAAGAATCTGCTCTCGACA+AGG - chr7.3:70275611-70275630 MS.gene62524:CDS 45.0%
AGAATCTGACGCTGTATCCA+TGG - chr7.3:70273728-70273747 MS.gene62524:CDS 45.0%
AGATTTCCCTGTGAAGGTTC+AGG - chr7.3:70274400-70274419 MS.gene62524:CDS 45.0%
ATTTCTCATGCTCCTGGAAC+CGG - chr7.3:70274673-70274692 MS.gene62524:CDS 45.0%
CAGAAGCGAATCATCCTTTC+CGG - chr7.3:70275168-70275187 MS.gene62524:CDS 45.0%
CCCTTTGTGCACATGAACAA+AGG + chr7.3:70275406-70275425 None:intergenic 45.0%
CCCTTTGTTCATGTGCACAA+AGG - chr7.3:70275402-70275421 MS.gene62524:intron 45.0%
CGATGCAAATTGCCAAGCAA+AGG - chr7.3:70275878-70275897 MS.gene62524:intron 45.0%
CTGTGAAGGTTCAGGAAGAA+AGG - chr7.3:70274408-70274427 MS.gene62524:CDS 45.0%
CTTTCTTCCTGAACCTTCAC+AGG + chr7.3:70274410-70274429 None:intergenic 45.0%
GCGGCTAAAGCAGTACTATT+TGG - chr7.3:70274208-70274227 MS.gene62524:CDS 45.0%
GCTGACCATAGTGTTTCTCA+AGG - chr7.3:70274705-70274724 MS.gene62524:CDS 45.0%
GTAAACAGGCTGAAAAGGAC+CGG - chr7.3:70276096-70276115 MS.gene62524:CDS 45.0%
TCTGACCAATCCTATACGCT+CGG + chr7.3:70276016-70276035 None:intergenic 45.0%
TTGTTAAGTGCGTGGTACTG+AGG - chr7.3:70276246-70276265 MS.gene62524:CDS 45.0%
! AACTGTGTTCGAGTTCAAGG+AGG - chr7.3:70273554-70273573 MS.gene62524:CDS 45.0%
! AGGAGGAGAACGAAGTGTTA+TGG - chr7.3:70273682-70273701 MS.gene62524:CDS 45.0%
! CAGAGAATGGTGGAGAACAA+AGG - chr7.3:70273369-70273388 MS.gene62524:CDS 45.0%
! CTTGCAATGAAGGCACAGTT+TGG - chr7.3:70274512-70274531 MS.gene62524:CDS 45.0%
! GAATGGTGGAGAACAAAGGA+AGG - chr7.3:70273373-70273392 MS.gene62524:CDS 45.0%
! GGACTGTTTGGTGACTTTGA+AGG - chr7.3:70275458-70275477 MS.gene62524:intron 45.0%
! GGAGGAGAACGAAGTGTTAT+GGG - chr7.3:70273683-70273702 MS.gene62524:CDS 45.0%
! TATGGTCAGCCTTGTCTTTC+CGG + chr7.3:70274695-70274714 None:intergenic 45.0%
! TGTTTGGTGACTTTGAAGGC+AGG - chr7.3:70275462-70275481 MS.gene62524:intron 45.0%
!! AGCTTCTAGGGTTGTACTTC+TGG - chr7.3:70275950-70275969 MS.gene62524:CDS 45.0%
!! TAGGATTGGTCAGAAGAGAG+TGG - chr7.3:70276022-70276041 MS.gene62524:CDS 45.0%
AAGCTATCAGCCGAGCGTAT+AGG - chr7.3:70276003-70276022 MS.gene62524:CDS 50.0%
AATGCCGATCCTTGCAGTGA+AGG - chr7.3:70274640-70274659 MS.gene62524:CDS 50.0%
ACATACCATCTGCTCGCTGA+AGG - chr7.3:70276050-70276069 MS.gene62524:CDS 50.0%
ACCAAACAGTCCCTTAACCC+AGG + chr7.3:70275450-70275469 None:intergenic 50.0%
ACTGAGGAAGGAGCGAGATA+TGG - chr7.3:70276262-70276281 MS.gene62524:CDS 50.0%
AGTGCAAAAGACCATGGAGG+AGG - chr7.3:70273662-70273681 MS.gene62524:CDS 50.0%
ATCAGCCGAGCGTATAGGAT+TGG - chr7.3:70276008-70276027 MS.gene62524:CDS 50.0%
CATACCATCTGCTCGCTGAA+GGG - chr7.3:70276051-70276070 MS.gene62524:CDS 50.0%
CGGAGTTGTGAACCTGTTTC+GGG - chr7.3:70275321-70275340 MS.gene62524:intron 50.0%
CTCACCACTAGTGGTAAGCA+CGG - chr7.3:70274062-70274081 MS.gene62524:CDS 50.0%
GAAACCGTGCTTACCACTAG+TGG + chr7.3:70274069-70274088 None:intergenic 50.0%
GCGGAGTTGTGAACCTGTTT+CGG - chr7.3:70275320-70275339 MS.gene62524:intron 50.0%
GCTCCTTGCGCAATGCTATT+GGG - chr7.3:70273503-70273522 MS.gene62524:CDS 50.0%
GGACTCATCGTCATTATCGG+CGG + chr7.3:70276153-70276172 None:intergenic 50.0%
GTCTGGCAAAGATCCTCCAA+AGG - chr7.3:70275827-70275846 MS.gene62524:intron 50.0%
TCCTGGGTTAAGGGACTGTT+TGG - chr7.3:70275446-70275465 MS.gene62524:intron 50.0%
TCGTGTTCACATCGCGATGA+TGG + chr7.3:70273970-70273989 None:intergenic 50.0%
TGCTCCTTGCGCAATGCTAT+TGG - chr7.3:70273502-70273521 MS.gene62524:CDS 50.0%
TGTCCCAATAGCATTGCGCA+AGG + chr7.3:70273509-70273528 None:intergenic 50.0%
! ACGGATTGCTTGACCTTTGG+AGG + chr7.3:70275843-70275862 None:intergenic 50.0%
! CTTGTTGTGTTGCCCGAAAC+AGG + chr7.3:70275336-70275355 None:intergenic 50.0%
! GTGTTCGAGTTCAAGGAGGA+TGG - chr7.3:70273558-70273577 MS.gene62524:CDS 50.0%
! TAAGTGCGTGGTACTGAGGA+AGG - chr7.3:70276250-70276269 MS.gene62524:CDS 50.0%
! TCTGGAATGGAGTTCCGGAA+AGG + chr7.3:70275185-70275204 None:intergenic 50.0%
ACAAGCTACACAGCCACCAC+CGG - chr7.3:70273590-70273609 MS.gene62524:CDS 55.0%
AGCTCAGGCGGTATCGTGTT+AGG + chr7.3:70275258-70275277 None:intergenic 55.0%
ATGCAGCCACCTTCACTGCA+AGG + chr7.3:70274652-70274671 None:intergenic 55.0%
CTCCTGGAACCGGAAAGACA+AGG - chr7.3:70274683-70274702 MS.gene62524:CDS 55.0%
GCAAAAGACCATGGAGGAGG+AGG - chr7.3:70273665-70273684 MS.gene62524:CDS 55.0%
GCCACCTTCACTGCAAGGAT+CGG + chr7.3:70274647-70274666 None:intergenic 55.0%
TAGTCCCTTCAGCGAGCAGA+TGG + chr7.3:70276058-70276077 None:intergenic 55.0%
TCTGCTCGCTGAAGGGACTA+TGG - chr7.3:70276058-70276077 MS.gene62524:CDS 55.0%
TGCTCTCGACAAGGATCAGC+TGG - chr7.3:70275620-70275639 MS.gene62524:CDS 55.0%
TGGAGACACCCCCATCGATA+TGG - chr7.3:70273619-70273638 MS.gene62524:CDS 55.0%
TTCGTTCTCCTCCTCCTCCA+TGG + chr7.3:70273676-70273695 None:intergenic 55.0%
! AGCCTTGTCTTTCCGGTTCC+AGG + chr7.3:70274688-70274707 None:intergenic 55.0%
CGCTGAAGGGACTATGGAGG+AGG - chr7.3:70276064-70276083 MS.gene62524:CDS 60.0%
GCCGATCCTTGCAGTGAAGG+TGG - chr7.3:70274643-70274662 MS.gene62524:CDS 60.0%
GCTCGCTGAAGGGACTATGG+AGG - chr7.3:70276061-70276080 MS.gene62524:CDS 60.0%
GGTGTCTCCACAGAAACCGG+TGG + chr7.3:70273609-70273628 None:intergenic 60.0%
GTCTCCACAGAAACCGGTGG+TGG + chr7.3:70273606-70273625 None:intergenic 60.0%
!! ACAGCCACCACCGGTTTCTG+TGG - chr7.3:70273599-70273618 MS.gene62524:CDS 60.0%
!! GGGGGTGTCTCCACAGAAAC+CGG + chr7.3:70273612-70273631 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 70273348 70276289 70273348 ID=MS.gene62524
chr7.3 mRNA 70273348 70276289 70273348 ID=MS.gene62524.t1;Parent=MS.gene62524
chr7.3 exon 70275888 70276289 70275888 ID=MS.gene62524.t1.exon1;Parent=MS.gene62524.t1
chr7.3 CDS 70275888 70276289 70275888 ID=cds.MS.gene62524.t1;Parent=MS.gene62524.t1
chr7.3 exon 70275563 70275718 70275563 ID=MS.gene62524.t1.exon2;Parent=MS.gene62524.t1
chr7.3 CDS 70275563 70275718 70275563 ID=cds.MS.gene62524.t1;Parent=MS.gene62524.t1
chr7.3 exon 70273348 70275243 70273348 ID=MS.gene62524.t1.exon3;Parent=MS.gene62524.t1
chr7.3 CDS 70273348 70275243 70273348 ID=cds.MS.gene62524.t1;Parent=MS.gene62524.t1
Gene Sequence

>MS.gene62524

ATGATGGAGGAATCAAGTAAACAGAGAATGGTGGAGAACAAAGGAAGGGATTATAAAGGTATAGCTAACATCCATATTGAGAAGAAGAAGGAATCTATTGATAACAATGGTTTGATACAAAGCGTCAAAAGCACACATTTTATGCGGAATGAGTTGCTCCTTGCGCAATGCTATTGGGACAGAAAGAATACCATGAAGAATGATTCAACTGTGTTCGAGTTCAAGGAGGATGGCGTTGATCGACAAGCTACACAGCCACCACCGGTTTCTGTGGAGACACCCCCATCGATATGGAACCTTAAGAAGGTGGAAAAAGTGCAAAAGACCATGGAGGAGGAGGAGAACGAAGTGTTATGGGATGAATTGGATACAGTTCTTAGAGAATCTGACGCTGTATCCATGGTAATCCTCCATTTTAAGACCATTTAAGTTTGAACTATGCATTTTAATTTTGTGAACTATAGGACTAGTGCTCAAGCTGATTTTAATTTTTATTTTAAACTGTTTATATGTTTAAACTTCGGTCTAAGCAATATCTATATGTTTATACATAGTGTGTTACTATCTACAGATTGGAAATTTAGGGACAAATGAAGCTACAAAAATAAAAAACGGAAGTCCATCATCGCGATGTGAACACGACACTTTTCTCGACGAAGAGATTGGTGTATATTGCAAATTGTGTGGTGTGGTTATTACTGAGATCAAATATATCTCACCACTAGTGGTAAGCACGGTTTCATATTGATTTATGTAAAATAACTTGGTTACTTTTCTGATTTTGGTCTTGAATAGTGAAATTTGAACACATCACTTTTGATTGCATTATGCAGCGATTAAATGCAAAGTATTGTGTACTTGCGGCTAAAGCAGTACTATTTGGAACATAACACTAATAAATTTGTTGCTGTTAGATTCAATAAGTTTCTGCTGTTAGATTCAAATTAAATTTCATATAGTTGTATGTTTGTTCTGATTTTACCTTGTGATATATATGTTGATAAGAGGCACTGATATTTTGCATACTTGTTTTACACGTGACACAGGTTGATAGATTTCCCTGTGAAGGTTCAGGAAGAAAGGCATCATTTGATGGAGTAAATGTTTCACTGTTTGATGGATCTCAATTCAATGTTTATGATAAAGATTCAGAAACTAATTTCTCTTGCAATGAAGGCACAGTTTGGGACCTAATTCCAGATTTGAAACAAACTTTATATCGTCATCAACAAGAAGGCTTTGAATTCATTTGGAAAAACTTGGCAGGAAGTGTCAAGCTTCAAAAGTTAAAGAATGCCGATCCTTGCAGTGAAGGTGGCTGCATTATTTCTCATGCTCCTGGAACCGGAAAGACAAGGCTGACCATAGTGTTTCTCAAGGCATATTTGAAAGCCTTTCCCAAATGCTTTCCGATTATTGTTGCTCCTGCTAGTATACTACTAACTTGGGAGGATGAATTCAAGAAATGGGACATCGGAGTTCCGTTTCACAATTTAAGCAATCCCGAGTTATCTGGTAAAGAACATGCTGATGCGGTTGAAACGTTTGATCAGTATAATACACAGCATAATATTAATGAAACGCGGATGACAAAAATGATTTCGTGGTTCAAAGAAACAAGCATTCTTGGAATCAGCTACAGTTTGTTTGGAAAAAAATGCCAAGATAAGAAAAAGCATGAAAATGTGAAGGGGAGAAAAGGAAACTGTGATATGCGAAAGATTCTGCTAAAAGCTCCTGGTTTGTTAGTTCTAGATGAAGGACACACACCAAGAAATCAAAGAAGTCATATTTGGAAGGTGTTGTCGAAGATTCAAGCGCAGAAGCGAATCATCCTTTCCGGAACTCCATTCCAGAACAATTTCTGGGAGCTTTACAGCACCTTAAGCTTAGTGAAGCGTTCTTTTCCTAACACGATACCGCCTGAGCTAAAAAGTTTTTGCCATAAGCAGGGACATAAATCATCTAAAAAGCGGAGTTGTGAACCTGTTTCGGGCAACACAACAAGAGACCCTTCTGATGATAAGATCAAGAAGCTGAAATTGCTAATGGATCCCTTTGTTCATGTGCACAAAGGGGCGATCCTTGAAAATAAGCTTCCTGGGTTAAGGGACTGTTTGGTGACTTTGAAGGCAGGTAGTTTGCAGAATGAAATTCTGAAGAGCATTAAACGTTCTAAGAACACAATATTTAACTTTGAACGTAAGATAGCATTGACATCCGTCCATCCATCGCTTTTCCTTGAATGTTCTCTTTCGGAAGAAGAAGAATCTGCTCTCGACAAGGATCAGCTGGAAAAGCTTAGACTGAACCCATATGAAGGTGTCAAAACAAAATTTTTGTTCGAGTTTGTTCGGCTCTGCGATGCTTTTCACGAGAAAGTTCTTGTGTTCAGCCAATTCCATGCGCCTTTACAATTAATTAAAGACCAATTAACCTCTGCTTTTAAATGGTCTGAGGGAAAGGAAGTGTTGTTCATGTCTGGCAAAGATCCTCCAAAGGTCAAGCAATCCGTAATCCATAGCTTCAACGATGCAAATTGCCAAGCAAAGGTTCTATTCGCATCTACAAAAGCTTGTTCTGAGGGAATTAGCTTAGTTGGAGCTTCTAGGGTTGTACTTCTGGATGTTGTATGGAATCCTTCAGTCGAAAGACAAGCTATCAGCCGAGCGTATAGGATTGGTCAGAAGAGAGTGGTATACACATACCATCTGCTCGCTGAAGGGACTATGGAGGAGGAAAAATATGGTAAACAGGCTGAAAAGGACCGGTTATCTGAGCTTGTTTTTTCAGAGAAAAATGCCGCCGATAATGACGATGAGTCCAAGAGCTGTGATATGAATTTTGAAGATAGAGTTCTTGATCATATGACTCAGCATGAAAATCTCGAAGACATGTTTGTTAAGTGCGTGGTACTGAGGAAGGAGCGAGATATGGTCTGA
Protein sequence

>MS.gene62524.t1

MMEESSKQRMVENKGRDYKGIANIHIEKKKESIDNNGLIQSVKSTHFMRNELLLAQCYWDRKNTMKNDSTVFEFKEDGVDRQATQPPPVSVETPPSIWNLKKVEKVQKTMEEEENEVLWDELDTVLRESDAVSMIGNLGTNEATKIKNGSPSSRCEHDTFLDEEIGVYCKLCGVVITEIKYISPLVVDRFPCEGSGRKASFDGVNVSLFDGSQFNVYDKDSETNFSCNEGTVWDLIPDLKQTLYRHQQEGFEFIWKNLAGSVKLQKLKNADPCSEGGCIISHAPGTGKTRLTIVFLKAYLKAFPKCFPIIVAPASILLTWEDEFKKWDIGVPFHNLSNPELSGKEHADAVETFDQYNTQHNINETRMTKMISWFKETSILGISYSLFGKKCQDKKKHENVKGRKGNCDMRKILLKAPGLLVLDEGHTPRNQRSHIWKVLSKIQAQKRIILSGTPFQNNFWELYSTLSLVKRSFPNTIPPELKSFCHKQGHKSSKKRSCEPVSGNTTRDPSDDKIKKLKLLMDPFVHVHKGAILENKLPGLRDCLVTLKAGSLQNEILKSIKRSKNTIFNFERKIALTSVHPSLFLECSLSEEEESALDKDQLEKLRLNPYEGVKTKFLFEFVRLCDAFHEKVLVFSQFHAPLQLIKDQLTSAFKWSEGKEVLFMSGKDPPKVKQSVIHSFNDANCQAKVLFASTKACSEGISLVGASRVVLLDVVWNPSVERQAISRAYRIGQKRVVYTYHLLAEGTMEEEKYGKQAEKDRLSELVFSEKNAADNDDESKSCDMNFEDRVLDHMTQHENLEDMFVKCVVLRKERDMV