Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62567.t1 | RHN40111.1 | 82.7 | 98 | 15 | 2 | 1 | 97 | 176 | 272 | 4.60E-30 | 141 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62567.t1 | A0A396GIW2 | 82.7 | 98 | 15 | 2 | 1 | 97 | 176 | 272 | 3.3e-30 | 141.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060426 | MS.gene62567 | 0.990239 | 1.06E-181 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62567.t1 | MTR_2g070860 | 78.571 | 42 | 9 | 0 | 88 | 129 | 118 | 159 | 2.02e-14 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 55 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAACCTTACCATCCCATTT+TGG | 0.238925 | 7.3:-68905748 | None:intergenic |
| ATCTCCTGAGCTGAGATGTT+GGG | 0.302898 | 7.3:-68905903 | None:intergenic |
| CTATTCCAGAAACACCAAAA+TGG | 0.307967 | 7.3:+68905734 | MS.gene62567:CDS |
| CCTTCCGTGGCAACTCTTAC+AGG | 0.323101 | 7.3:-68905790 | None:intergenic |
| TCACTACCATCTTCAATTAC+TGG | 0.376171 | 7.3:-68905850 | None:intergenic |
| CCTCCTGACCAAGGGTGTTC+AGG | 0.383569 | 7.3:+68906041 | MS.gene62567:CDS |
| GATGAAGACCCTAGGGTTGA+TGG | 0.395963 | 7.3:+68905823 | MS.gene62567:CDS |
| GACCAAGGGTGTTCAGGCGG+CGG | 0.423334 | 7.3:+68906047 | MS.gene62567:CDS |
| TAGTGATGATGAAGATGTTC+AGG | 0.431216 | 7.3:+68905867 | MS.gene62567:CDS |
| TGTAAGAGTTGCCACGGAAG+GGG | 0.436350 | 7.3:+68905792 | MS.gene62567:CDS |
| CCGCCTGAACACCCTTGGTC+AGG | 0.440105 | 7.3:-68906044 | None:intergenic |
| ACCAAGGGTGTTCAGGCGGC+GGG | 0.453675 | 7.3:+68906048 | MS.gene62567:CDS |
| ATGAGGATGTACTCCGAACT+AGG | 0.464179 | 7.3:-68905958 | None:intergenic |
| CAACTTTGTGCCGAGTTGAT+CGG | 0.465207 | 7.3:+68906135 | MS.gene62567:CDS |
| GAACTAGGGTACCTGGTAGA+TGG | 0.478163 | 7.3:-68905943 | None:intergenic |
| TATTCCAGAAACACCAAAAT+GGG | 0.484443 | 7.3:+68905735 | MS.gene62567:CDS |
| ACCCGCCGCCTGAACACCCT+TGG | 0.495868 | 7.3:-68906049 | None:intergenic |
| AATCCCCAACATCTCAGCTC+AGG | 0.503790 | 7.3:+68905899 | MS.gene62567:CDS |
| ATCTACCAGGTACCCTAGTT+CGG | 0.508012 | 7.3:+68905945 | MS.gene62567:CDS |
| CAAGTATACATGTGTGTATG+TGG | 0.508521 | 7.3:+68906100 | MS.gene62567:CDS |
| GATCTCCTGAGCTGAGATGT+TGG | 0.512218 | 7.3:-68905904 | None:intergenic |
| AACACCAAAATGGGATGGTA+AGG | 0.521901 | 7.3:+68905744 | MS.gene62567:CDS |
| TCTCCTGAGCTGAGATGTTG+GGG | 0.523063 | 7.3:-68905902 | None:intergenic |
| ACTGGCTCTCCATCAACCCT+AGG | 0.537342 | 7.3:-68905832 | None:intergenic |
| GAGATCTTGATGAAGACCCT+AGG | 0.554127 | 7.3:+68905815 | MS.gene62567:CDS |
| CAGAGTCGTGGTTTGACGAA+GGG | 0.556569 | 7.3:+68905992 | MS.gene62567:CDS |
| TCCGTTGTGTGCGGATACAA+CGG | 0.559620 | 7.3:+68906072 | MS.gene62567:CDS |
| CTGTAAGAGTTGCCACGGAA+GGG | 0.560141 | 7.3:+68905791 | MS.gene62567:CDS |
| CCTGTAAGAGTTGCCACGGA+AGG | 0.568211 | 7.3:+68905790 | MS.gene62567:CDS |
| ACTCTTACAGGAGGAGTGAT+CGG | 0.576759 | 7.3:-68905778 | None:intergenic |
| CCTGAACACCCTTGGTCAGG+AGG | 0.577539 | 7.3:-68906041 | None:intergenic |
| GGAGAGCCAGTAATTGAAGA+TGG | 0.578285 | 7.3:+68905844 | MS.gene62567:CDS |
| TTGTGCCGAGTTGATCGGCA+TGG | 0.580056 | 7.3:+68906140 | MS.gene62567:CDS |
| GTACTCCGAACTAGGGTACC+TGG | 0.585306 | 7.3:-68905950 | None:intergenic |
| TCAGAGTCGTGGTTTGACGA+AGG | 0.596520 | 7.3:+68905991 | MS.gene62567:CDS |
| TCCTCCTGTAAGAGTTGCCA+CGG | 0.598941 | 7.3:+68905786 | MS.gene62567:CDS |
| CCTGACCAAGGGTGTTCAGG+CGG | 0.600536 | 7.3:+68906044 | MS.gene62567:CDS |
| GCGATCCATGCCGATCAACT+CGG | 0.603085 | 7.3:-68906145 | None:intergenic |
| AGATCTTGATGAAGACCCTA+GGG | 0.607768 | 7.3:+68905816 | MS.gene62567:CDS |
| GTAAGAGTTGCCACGGAAGG+GGG | 0.611554 | 7.3:+68905793 | MS.gene62567:CDS |
| CTGGCTCTCCATCAACCCTA+GGG | 0.612809 | 7.3:-68905831 | None:intergenic |
| TCAGGAGGACTTTCGACACA+AGG | 0.613743 | 7.3:-68906026 | None:intergenic |
| TCGAAAGTCCTCCTGACCAA+GGG | 0.614090 | 7.3:+68906033 | MS.gene62567:CDS |
| CCAGAAACACCAAAATGGGA+TGG | 0.615430 | 7.3:+68905739 | MS.gene62567:CDS |
| GTCGAAAGTCCTCCTGACCA+AGG | 0.615599 | 7.3:+68906032 | MS.gene62567:CDS |
| GGTTTGACGAAGGGTTCGAT+CGG | 0.618193 | 7.3:+68906001 | MS.gene62567:CDS |
| GAGATCGTCAACCATCTACC+AGG | 0.624707 | 7.3:+68905932 | MS.gene62567:CDS |
| GCGGCGGGTTCCGTTGTGTG+CGG | 0.627648 | 7.3:+68906063 | MS.gene62567:CDS |
| GATGATGAAGATGTTCAGGA+TGG | 0.628053 | 7.3:+68905871 | MS.gene62567:CDS |
| TGAGGATGTACTCCGAACTA+GGG | 0.633295 | 7.3:-68905957 | None:intergenic |
| TCACTGATGAATCAGAGTCG+TGG | 0.662711 | 7.3:+68905980 | MS.gene62567:CDS |
| ACTCTGATTCATCAGTGATG+AGG | 0.664573 | 7.3:-68905975 | None:intergenic |
| TCCGTGGCAACTCTTACAGG+AGG | 0.668888 | 7.3:-68905787 | None:intergenic |
| GCCGTTGTATCCGCACACAA+CGG | 0.683572 | 7.3:-68906073 | None:intergenic |
| GTTGTGTGCGGATACAACGG+CGG | 0.764729 | 7.3:+68906075 | MS.gene62567:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATTCCAGAAACACCAAAAT+GGG | + | chr7.3:68905735-68905754 | MS.gene62567:CDS | 30.0% | |
| CAAGTATACATGTGTGTATG+TGG | + | chr7.3:68906100-68906119 | MS.gene62567:CDS | 35.0% | |
| CTATTCCAGAAACACCAAAA+TGG | + | chr7.3:68905734-68905753 | MS.gene62567:CDS | 35.0% | |
| TCACTACCATCTTCAATTAC+TGG | - | chr7.3:68905853-68905872 | None:intergenic | 35.0% | |
| ! | TAGTGATGATGAAGATGTTC+AGG | + | chr7.3:68905867-68905886 | MS.gene62567:CDS | 35.0% |
| AACACCAAAATGGGATGGTA+AGG | + | chr7.3:68905744-68905763 | MS.gene62567:CDS | 40.0% | |
| ACTCTGATTCATCAGTGATG+AGG | - | chr7.3:68905978-68905997 | None:intergenic | 40.0% | |
| AGATCTTGATGAAGACCCTA+GGG | + | chr7.3:68905816-68905835 | MS.gene62567:CDS | 40.0% | |
| GATGATGAAGATGTTCAGGA+TGG | + | chr7.3:68905871-68905890 | MS.gene62567:CDS | 40.0% | |
| ! | TCAACCTTACCATCCCATTT+TGG | - | chr7.3:68905751-68905770 | None:intergenic | 40.0% |
| ACTCTTACAGGAGGAGTGAT+CGG | - | chr7.3:68905781-68905800 | None:intergenic | 45.0% | |
| ATCTACCAGGTACCCTAGTT+CGG | + | chr7.3:68905945-68905964 | MS.gene62567:CDS | 45.0% | |
| ATCTCCTGAGCTGAGATGTT+GGG | - | chr7.3:68905906-68905925 | None:intergenic | 45.0% | |
| ATGAGGATGTACTCCGAACT+AGG | - | chr7.3:68905961-68905980 | None:intergenic | 45.0% | |
| CCAGAAACACCAAAATGGGA+TGG | + | chr7.3:68905739-68905758 | MS.gene62567:CDS | 45.0% | |
| GAGATCTTGATGAAGACCCT+AGG | + | chr7.3:68905815-68905834 | MS.gene62567:CDS | 45.0% | |
| GGAGAGCCAGTAATTGAAGA+TGG | + | chr7.3:68905844-68905863 | MS.gene62567:CDS | 45.0% | |
| TCACTGATGAATCAGAGTCG+TGG | + | chr7.3:68905980-68905999 | MS.gene62567:CDS | 45.0% | |
| TGAGGATGTACTCCGAACTA+GGG | - | chr7.3:68905960-68905979 | None:intergenic | 45.0% | |
| !! | CAACTTTGTGCCGAGTTGAT+CGG | + | chr7.3:68906135-68906154 | MS.gene62567:CDS | 45.0% |
| !!! | CCATCCCATTTTGGTGTTTC+TGG | - | chr7.3:68905742-68905761 | None:intergenic | 45.0% |
| AATCCCCAACATCTCAGCTC+AGG | + | chr7.3:68905899-68905918 | MS.gene62567:CDS | 50.0% | |
| CAGAGTCGTGGTTTGACGAA+GGG | + | chr7.3:68905992-68906011 | MS.gene62567:CDS | 50.0% | |
| CTGTAAGAGTTGCCACGGAA+GGG | + | chr7.3:68905791-68905810 | MS.gene62567:CDS | 50.0% | |
| GAGATCGTCAACCATCTACC+AGG | + | chr7.3:68905932-68905951 | MS.gene62567:CDS | 50.0% | |
| GATCTCCTGAGCTGAGATGT+TGG | - | chr7.3:68905907-68905926 | None:intergenic | 50.0% | |
| GATGAAGACCCTAGGGTTGA+TGG | + | chr7.3:68905823-68905842 | MS.gene62567:CDS | 50.0% | |
| GGTTTGACGAAGGGTTCGAT+CGG | + | chr7.3:68906001-68906020 | MS.gene62567:CDS | 50.0% | |
| TCAGAGTCGTGGTTTGACGA+AGG | + | chr7.3:68905991-68906010 | MS.gene62567:CDS | 50.0% | |
| TCAGGAGGACTTTCGACACA+AGG | - | chr7.3:68906029-68906048 | None:intergenic | 50.0% | |
| TCCGTTGTGTGCGGATACAA+CGG | + | chr7.3:68906072-68906091 | MS.gene62567:CDS | 50.0% | |
| TCCTCCTGTAAGAGTTGCCA+CGG | + | chr7.3:68905786-68905805 | MS.gene62567:CDS | 50.0% | |
| TCGAAAGTCCTCCTGACCAA+GGG | + | chr7.3:68906033-68906052 | MS.gene62567:CDS | 50.0% | |
| TCTCCTGAGCTGAGATGTTG+GGG | - | chr7.3:68905905-68905924 | None:intergenic | 50.0% | |
| TGTAAGAGTTGCCACGGAAG+GGG | + | chr7.3:68905792-68905811 | MS.gene62567:CDS | 50.0% | |
| ! | GAACTAGGGTACCTGGTAGA+TGG | - | chr7.3:68905946-68905965 | None:intergenic | 50.0% |
| ATCAAGATCTCCCCCTTCCG+TGG | - | chr7.3:68905806-68905825 | None:intergenic | 55.0% | |
| CCTGTAAGAGTTGCCACGGA+AGG | + | chr7.3:68905790-68905809 | MS.gene62567:CDS | 55.0% | |
| CCTTCCGTGGCAACTCTTAC+AGG | - | chr7.3:68905793-68905812 | None:intergenic | 55.0% | |
| GCCGTTGTATCCGCACACAA+CGG | - | chr7.3:68906076-68906095 | None:intergenic | 55.0% | |
| GCGATCCATGCCGATCAACT+CGG | - | chr7.3:68906148-68906167 | None:intergenic | 55.0% | |
| GTAAGAGTTGCCACGGAAGG+GGG | + | chr7.3:68905793-68905812 | MS.gene62567:CDS | 55.0% | |
| GTCGAAAGTCCTCCTGACCA+AGG | + | chr7.3:68906032-68906051 | MS.gene62567:CDS | 55.0% | |
| GTTGTGTGCGGATACAACGG+CGG | + | chr7.3:68906075-68906094 | MS.gene62567:CDS | 55.0% | |
| TCCGTGGCAACTCTTACAGG+AGG | - | chr7.3:68905790-68905809 | None:intergenic | 55.0% | |
| ! | ACTGGCTCTCCATCAACCCT+AGG | - | chr7.3:68905835-68905854 | None:intergenic | 55.0% |
| ! | CTGGCTCTCCATCAACCCTA+GGG | - | chr7.3:68905834-68905853 | None:intergenic | 55.0% |
| ! | GTACTCCGAACTAGGGTACC+TGG | - | chr7.3:68905953-68905972 | None:intergenic | 55.0% |
| !! | TTGTGCCGAGTTGATCGGCA+TGG | + | chr7.3:68906140-68906159 | MS.gene62567:CDS | 55.0% |
| CCTGAACACCCTTGGTCAGG+AGG | - | chr7.3:68906044-68906063 | None:intergenic | 60.0% | |
| !! | CCTCCTGACCAAGGGTGTTC+AGG | + | chr7.3:68906041-68906060 | MS.gene62567:CDS | 60.0% |
| !! | CCTGACCAAGGGTGTTCAGG+CGG | + | chr7.3:68906044-68906063 | MS.gene62567:CDS | 60.0% |
| CCGCCTGAACACCCTTGGTC+AGG | - | chr7.3:68906047-68906066 | None:intergenic | 65.0% | |
| !! | ACCAAGGGTGTTCAGGCGGC+GGG | + | chr7.3:68906048-68906067 | MS.gene62567:CDS | 65.0% |
| !! | GACCAAGGGTGTTCAGGCGG+CGG | + | chr7.3:68906047-68906066 | MS.gene62567:CDS | 65.0% |
| ACCCGCCGCCTGAACACCCT+TGG | - | chr7.3:68906052-68906071 | None:intergenic | 70.0% | |
| ! | GCGGCGGGTTCCGTTGTGTG+CGG | + | chr7.3:68906063-68906082 | MS.gene62567:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 68905721 | 68906173 | 68905721 | ID=MS.gene62567 |
| chr7.3 | mRNA | 68905721 | 68906173 | 68905721 | ID=MS.gene62567.t1;Parent=MS.gene62567 |
| chr7.3 | exon | 68905721 | 68906173 | 68905721 | ID=MS.gene62567.t1.exon1;Parent=MS.gene62567.t1 |
| chr7.3 | CDS | 68905721 | 68906173 | 68905721 | ID=cds.MS.gene62567.t1;Parent=MS.gene62567.t1 |
| Gene Sequence |
| Protein sequence |
