Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060426.t1 | RHN40111.1 | 82.7 | 98 | 15 | 2 | 1 | 97 | 176 | 272 | 4.60E-30 | 141 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060426.t1 | A0A396GIW2 | 82.7 | 98 | 15 | 2 | 1 | 97 | 176 | 272 | 3.3e-30 | 141.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene060426 | 0.810098 | 1.36E-50 | -1.69E-46 |
| MS.gene060426 | MS.gene064969 | 0.800774 | 1.23E-48 | -1.69E-46 |
| MS.gene060426 | MS.gene08675 | 0.814126 | 1.81E-51 | -1.69E-46 |
| MS.gene060426 | MS.gene38368 | 0.800725 | 1.26E-48 | -1.69E-46 |
| MS.gene060426 | MS.gene44909 | 0.818857 | 1.57E-52 | -1.69E-46 |
| MS.gene060426 | MS.gene62567 | 0.990239 | 1.06E-181 | -1.69E-46 |
| MS.gene060426 | MS.gene72748 | 0.805482 | 1.31E-49 | -1.69E-46 |
| MS.gene060426 | MS.gene73666 | 0.840659 | 7.61E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060426.t1 | MTR_2g070860 | 78.571 | 42 | 9 | 0 | 88 | 129 | 118 | 159 | 2.02e-14 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 55 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAACCTTACCATCCCATTT+TGG | 0.238925 | 7.1:-66361563 | None:intergenic |
| ATCTCCTGAGCTGAGATGTT+GGG | 0.302898 | 7.1:-66361718 | None:intergenic |
| CTATTCCAGAAACACCAAAA+TGG | 0.307967 | 7.1:+66361549 | MS.gene060426:CDS |
| CCTTCCGTGGCAACTCTTAC+AGG | 0.323101 | 7.1:-66361605 | None:intergenic |
| TCACTACCATCTTCAATTAC+TGG | 0.376171 | 7.1:-66361665 | None:intergenic |
| CCTCCTGACCAAGGGTGTTC+AGG | 0.383569 | 7.1:+66361856 | MS.gene060426:CDS |
| GATGAAGACCCTAGGGTTGA+TGG | 0.395963 | 7.1:+66361638 | MS.gene060426:CDS |
| GACCAAGGGTGTTCAGGCGG+CGG | 0.423334 | 7.1:+66361862 | MS.gene060426:CDS |
| TAGTGATGATGAAGATGTTC+AGG | 0.431216 | 7.1:+66361682 | MS.gene060426:CDS |
| TGTAAGAGTTGCCACGGAAG+GGG | 0.436350 | 7.1:+66361607 | MS.gene060426:CDS |
| CCGCCTGAACACCCTTGGTC+AGG | 0.440105 | 7.1:-66361859 | None:intergenic |
| ACCAAGGGTGTTCAGGCGGC+GGG | 0.453675 | 7.1:+66361863 | MS.gene060426:CDS |
| ATGAGGATGTACTCCGAACT+AGG | 0.464179 | 7.1:-66361773 | None:intergenic |
| CAACTTTGTGCCGAGTTGAT+CGG | 0.465207 | 7.1:+66361950 | MS.gene060426:CDS |
| GAACTAGGGTACCTGGTAGA+TGG | 0.478163 | 7.1:-66361758 | None:intergenic |
| TATTCCAGAAACACCAAAAT+GGG | 0.484443 | 7.1:+66361550 | MS.gene060426:CDS |
| ACCCGCCGCCTGAACACCCT+TGG | 0.495868 | 7.1:-66361864 | None:intergenic |
| AATCCCCAACATCTCAGCTC+AGG | 0.503790 | 7.1:+66361714 | MS.gene060426:CDS |
| ATCTACCAGGTACCCTAGTT+CGG | 0.508012 | 7.1:+66361760 | MS.gene060426:CDS |
| CAAGTATACATGTGTGTATG+TGG | 0.508521 | 7.1:+66361915 | MS.gene060426:CDS |
| GATCTCCTGAGCTGAGATGT+TGG | 0.512218 | 7.1:-66361719 | None:intergenic |
| AACACCAAAATGGGATGGTA+AGG | 0.521901 | 7.1:+66361559 | MS.gene060426:CDS |
| TCTCCTGAGCTGAGATGTTG+GGG | 0.523063 | 7.1:-66361717 | None:intergenic |
| ACTGGCTCTCCATCAACCCT+AGG | 0.537342 | 7.1:-66361647 | None:intergenic |
| GAGATCTTGATGAAGACCCT+AGG | 0.554127 | 7.1:+66361630 | MS.gene060426:CDS |
| CAGAGTCGTGGTTTGACGAA+GGG | 0.556569 | 7.1:+66361807 | MS.gene060426:CDS |
| TCCGTTGTGTGCGGATACAA+CGG | 0.559620 | 7.1:+66361887 | MS.gene060426:CDS |
| CTGTAAGAGTTGCCACGGAA+GGG | 0.560141 | 7.1:+66361606 | MS.gene060426:CDS |
| CCTGTAAGAGTTGCCACGGA+AGG | 0.568211 | 7.1:+66361605 | MS.gene060426:CDS |
| ACTCTTACAGGAGGAGTGAT+CGG | 0.576759 | 7.1:-66361593 | None:intergenic |
| CCTGAACACCCTTGGTCAGG+AGG | 0.577539 | 7.1:-66361856 | None:intergenic |
| GGAGAGCCAGTAATTGAAGA+TGG | 0.578285 | 7.1:+66361659 | MS.gene060426:CDS |
| TTGTGCCGAGTTGATCGGCA+TGG | 0.580056 | 7.1:+66361955 | MS.gene060426:CDS |
| GTACTCCGAACTAGGGTACC+TGG | 0.585306 | 7.1:-66361765 | None:intergenic |
| TCAGAGTCGTGGTTTGACGA+AGG | 0.596520 | 7.1:+66361806 | MS.gene060426:CDS |
| TCCTCCTGTAAGAGTTGCCA+CGG | 0.598941 | 7.1:+66361601 | MS.gene060426:CDS |
| CCTGACCAAGGGTGTTCAGG+CGG | 0.600536 | 7.1:+66361859 | MS.gene060426:CDS |
| GCGATCCATGCCGATCAACT+CGG | 0.603085 | 7.1:-66361960 | None:intergenic |
| AGATCTTGATGAAGACCCTA+GGG | 0.607768 | 7.1:+66361631 | MS.gene060426:CDS |
| GTAAGAGTTGCCACGGAAGG+GGG | 0.611554 | 7.1:+66361608 | MS.gene060426:CDS |
| CTGGCTCTCCATCAACCCTA+GGG | 0.612809 | 7.1:-66361646 | None:intergenic |
| TCAGGAGGACTTTCGACACA+AGG | 0.613743 | 7.1:-66361841 | None:intergenic |
| TCGAAAGTCCTCCTGACCAA+GGG | 0.614090 | 7.1:+66361848 | MS.gene060426:CDS |
| CCAGAAACACCAAAATGGGA+TGG | 0.615430 | 7.1:+66361554 | MS.gene060426:CDS |
| GTCGAAAGTCCTCCTGACCA+AGG | 0.615599 | 7.1:+66361847 | MS.gene060426:CDS |
| GGTTTGACGAAGGGTTCGAT+CGG | 0.618193 | 7.1:+66361816 | MS.gene060426:CDS |
| GAGATCGTCAACCATCTACC+AGG | 0.624707 | 7.1:+66361747 | MS.gene060426:CDS |
| GCGGCGGGTTCCGTTGTGTG+CGG | 0.627648 | 7.1:+66361878 | MS.gene060426:CDS |
| GATGATGAAGATGTTCAGGA+TGG | 0.628053 | 7.1:+66361686 | MS.gene060426:CDS |
| TGAGGATGTACTCCGAACTA+GGG | 0.633295 | 7.1:-66361772 | None:intergenic |
| TCACTGATGAATCAGAGTCG+TGG | 0.662711 | 7.1:+66361795 | MS.gene060426:CDS |
| ACTCTGATTCATCAGTGATG+AGG | 0.664573 | 7.1:-66361790 | None:intergenic |
| TCCGTGGCAACTCTTACAGG+AGG | 0.668888 | 7.1:-66361602 | None:intergenic |
| GCCGTTGTATCCGCACACAA+CGG | 0.683572 | 7.1:-66361888 | None:intergenic |
| GTTGTGTGCGGATACAACGG+CGG | 0.764729 | 7.1:+66361890 | MS.gene060426:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATTCCAGAAACACCAAAAT+GGG | + | chr7.1:66361550-66361569 | MS.gene060426:CDS | 30.0% | |
| CAAGTATACATGTGTGTATG+TGG | + | chr7.1:66361915-66361934 | MS.gene060426:CDS | 35.0% | |
| CTATTCCAGAAACACCAAAA+TGG | + | chr7.1:66361549-66361568 | MS.gene060426:CDS | 35.0% | |
| TCACTACCATCTTCAATTAC+TGG | - | chr7.1:66361668-66361687 | None:intergenic | 35.0% | |
| ! | TAGTGATGATGAAGATGTTC+AGG | + | chr7.1:66361682-66361701 | MS.gene060426:CDS | 35.0% |
| AACACCAAAATGGGATGGTA+AGG | + | chr7.1:66361559-66361578 | MS.gene060426:CDS | 40.0% | |
| ACTCTGATTCATCAGTGATG+AGG | - | chr7.1:66361793-66361812 | None:intergenic | 40.0% | |
| AGATCTTGATGAAGACCCTA+GGG | + | chr7.1:66361631-66361650 | MS.gene060426:CDS | 40.0% | |
| GATGATGAAGATGTTCAGGA+TGG | + | chr7.1:66361686-66361705 | MS.gene060426:CDS | 40.0% | |
| ! | TCAACCTTACCATCCCATTT+TGG | - | chr7.1:66361566-66361585 | None:intergenic | 40.0% |
| ACTCTTACAGGAGGAGTGAT+CGG | - | chr7.1:66361596-66361615 | None:intergenic | 45.0% | |
| ATCTACCAGGTACCCTAGTT+CGG | + | chr7.1:66361760-66361779 | MS.gene060426:CDS | 45.0% | |
| ATCTCCTGAGCTGAGATGTT+GGG | - | chr7.1:66361721-66361740 | None:intergenic | 45.0% | |
| ATGAGGATGTACTCCGAACT+AGG | - | chr7.1:66361776-66361795 | None:intergenic | 45.0% | |
| CCAGAAACACCAAAATGGGA+TGG | + | chr7.1:66361554-66361573 | MS.gene060426:CDS | 45.0% | |
| GAGATCTTGATGAAGACCCT+AGG | + | chr7.1:66361630-66361649 | MS.gene060426:CDS | 45.0% | |
| GGAGAGCCAGTAATTGAAGA+TGG | + | chr7.1:66361659-66361678 | MS.gene060426:CDS | 45.0% | |
| TCACTGATGAATCAGAGTCG+TGG | + | chr7.1:66361795-66361814 | MS.gene060426:CDS | 45.0% | |
| TGAGGATGTACTCCGAACTA+GGG | - | chr7.1:66361775-66361794 | None:intergenic | 45.0% | |
| !! | CAACTTTGTGCCGAGTTGAT+CGG | + | chr7.1:66361950-66361969 | MS.gene060426:CDS | 45.0% |
| !!! | CCATCCCATTTTGGTGTTTC+TGG | - | chr7.1:66361557-66361576 | None:intergenic | 45.0% |
| AATCCCCAACATCTCAGCTC+AGG | + | chr7.1:66361714-66361733 | MS.gene060426:CDS | 50.0% | |
| CAGAGTCGTGGTTTGACGAA+GGG | + | chr7.1:66361807-66361826 | MS.gene060426:CDS | 50.0% | |
| CTGTAAGAGTTGCCACGGAA+GGG | + | chr7.1:66361606-66361625 | MS.gene060426:CDS | 50.0% | |
| GAGATCGTCAACCATCTACC+AGG | + | chr7.1:66361747-66361766 | MS.gene060426:CDS | 50.0% | |
| GATCTCCTGAGCTGAGATGT+TGG | - | chr7.1:66361722-66361741 | None:intergenic | 50.0% | |
| GATGAAGACCCTAGGGTTGA+TGG | + | chr7.1:66361638-66361657 | MS.gene060426:CDS | 50.0% | |
| GGTTTGACGAAGGGTTCGAT+CGG | + | chr7.1:66361816-66361835 | MS.gene060426:CDS | 50.0% | |
| TCAGAGTCGTGGTTTGACGA+AGG | + | chr7.1:66361806-66361825 | MS.gene060426:CDS | 50.0% | |
| TCAGGAGGACTTTCGACACA+AGG | - | chr7.1:66361844-66361863 | None:intergenic | 50.0% | |
| TCCGTTGTGTGCGGATACAA+CGG | + | chr7.1:66361887-66361906 | MS.gene060426:CDS | 50.0% | |
| TCCTCCTGTAAGAGTTGCCA+CGG | + | chr7.1:66361601-66361620 | MS.gene060426:CDS | 50.0% | |
| TCGAAAGTCCTCCTGACCAA+GGG | + | chr7.1:66361848-66361867 | MS.gene060426:CDS | 50.0% | |
| TCTCCTGAGCTGAGATGTTG+GGG | - | chr7.1:66361720-66361739 | None:intergenic | 50.0% | |
| TGTAAGAGTTGCCACGGAAG+GGG | + | chr7.1:66361607-66361626 | MS.gene060426:CDS | 50.0% | |
| ! | GAACTAGGGTACCTGGTAGA+TGG | - | chr7.1:66361761-66361780 | None:intergenic | 50.0% |
| ATCAAGATCTCCCCCTTCCG+TGG | - | chr7.1:66361621-66361640 | None:intergenic | 55.0% | |
| CCTGTAAGAGTTGCCACGGA+AGG | + | chr7.1:66361605-66361624 | MS.gene060426:CDS | 55.0% | |
| CCTTCCGTGGCAACTCTTAC+AGG | - | chr7.1:66361608-66361627 | None:intergenic | 55.0% | |
| GCCGTTGTATCCGCACACAA+CGG | - | chr7.1:66361891-66361910 | None:intergenic | 55.0% | |
| GCGATCCATGCCGATCAACT+CGG | - | chr7.1:66361963-66361982 | None:intergenic | 55.0% | |
| GTAAGAGTTGCCACGGAAGG+GGG | + | chr7.1:66361608-66361627 | MS.gene060426:CDS | 55.0% | |
| GTCGAAAGTCCTCCTGACCA+AGG | + | chr7.1:66361847-66361866 | MS.gene060426:CDS | 55.0% | |
| GTTGTGTGCGGATACAACGG+CGG | + | chr7.1:66361890-66361909 | MS.gene060426:CDS | 55.0% | |
| TCCGTGGCAACTCTTACAGG+AGG | - | chr7.1:66361605-66361624 | None:intergenic | 55.0% | |
| ! | ACTGGCTCTCCATCAACCCT+AGG | - | chr7.1:66361650-66361669 | None:intergenic | 55.0% |
| ! | CTGGCTCTCCATCAACCCTA+GGG | - | chr7.1:66361649-66361668 | None:intergenic | 55.0% |
| ! | GTACTCCGAACTAGGGTACC+TGG | - | chr7.1:66361768-66361787 | None:intergenic | 55.0% |
| !! | TTGTGCCGAGTTGATCGGCA+TGG | + | chr7.1:66361955-66361974 | MS.gene060426:CDS | 55.0% |
| CCTGAACACCCTTGGTCAGG+AGG | - | chr7.1:66361859-66361878 | None:intergenic | 60.0% | |
| !! | CCTCCTGACCAAGGGTGTTC+AGG | + | chr7.1:66361856-66361875 | MS.gene060426:CDS | 60.0% |
| !! | CCTGACCAAGGGTGTTCAGG+CGG | + | chr7.1:66361859-66361878 | MS.gene060426:CDS | 60.0% |
| CCGCCTGAACACCCTTGGTC+AGG | - | chr7.1:66361862-66361881 | None:intergenic | 65.0% | |
| !! | ACCAAGGGTGTTCAGGCGGC+GGG | + | chr7.1:66361863-66361882 | MS.gene060426:CDS | 65.0% |
| !! | GACCAAGGGTGTTCAGGCGG+CGG | + | chr7.1:66361862-66361881 | MS.gene060426:CDS | 65.0% |
| ACCCGCCGCCTGAACACCCT+TGG | - | chr7.1:66361867-66361886 | None:intergenic | 70.0% | |
| ! | GCGGCGGGTTCCGTTGTGTG+CGG | + | chr7.1:66361878-66361897 | MS.gene060426:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 66361536 | 66361988 | 66361536 | ID=MS.gene060426 |
| chr7.1 | mRNA | 66361536 | 66361988 | 66361536 | ID=MS.gene060426.t1;Parent=MS.gene060426 |
| chr7.1 | exon | 66361536 | 66361988 | 66361536 | ID=MS.gene060426.t1.exon1;Parent=MS.gene060426.t1 |
| chr7.1 | CDS | 66361536 | 66361988 | 66361536 | ID=cds.MS.gene060426.t1;Parent=MS.gene060426.t1 |
| Gene Sequence |
| Protein sequence |
