AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62673

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62673.t1 MTR_8g105780 98.922 371 4 0 1 371 1 371 0.0 761
MS.gene62673.t1 MTR_5g029680 45.956 272 127 7 22 276 32 300 4.43e-67 220
MS.gene62673.t1 MTR_4g077970 42.238 277 152 3 13 282 3 278 1.49e-66 218
MS.gene62673.t1 MTR_1g102860 50.000 198 89 4 22 219 40 227 8.45e-56 186
MS.gene62673.t1 MTR_7g095680 48.454 194 96 2 22 215 49 238 1.87e-53 181
MS.gene62673.t1 MTR_6g043060 45.794 214 103 6 1 214 1 201 5.23e-50 171
MS.gene62673.t1 MTR_3g104550 45.876 194 98 2 22 215 46 232 7.95e-50 172
MS.gene62673.t1 MTR_8g087540 45.000 200 99 3 14 211 4 194 7.62e-49 169
MS.gene62673.t1 MTR_1g061170 41.739 230 124 4 20 248 47 267 5.61e-48 169
MS.gene62673.t1 MTR_1g071360 45.699 186 83 3 22 207 32 199 6.10e-47 163
MS.gene62673.t1 MTR_1g071360 45.699 186 83 3 22 207 29 196 1.13e-46 163
MS.gene62673.t1 MTR_2g100680 44.724 199 99 4 12 210 21 208 1.73e-46 164
MS.gene62673.t1 MTR_2g017890 44.949 198 97 3 12 205 5 194 4.49e-46 162
MS.gene62673.t1 MTR_6g086805 46.193 197 95 4 20 216 9 194 2.30e-45 159
MS.gene62673.t1 MTR_8g083100 47.917 192 87 6 23 214 23 201 9.51e-45 160
MS.gene62673.t1 MTR_2g100670 43.627 204 98 5 4 207 21 207 6.53e-44 154
MS.gene62673.t1 MTR_2g100670 45.263 190 93 4 18 207 137 315 5.90e-43 154
MS.gene62673.t1 MTR_5g017470 42.373 177 74 2 21 170 8 183 1.25e-33 126
MS.gene62673.t1 MTR_5g089170 60.417 96 37 1 21 116 24 118 2.69e-33 124
MS.gene62673.t1 MTR_3g101870 62.766 94 34 1 21 114 23 115 2.71e-33 127
MS.gene62673.t1 MTR_5g010680 59.574 94 37 1 21 114 23 115 4.36e-33 127
MS.gene62673.t1 MTR_0020s0020 60.638 94 36 1 21 114 32 124 4.80e-32 123
MS.gene62673.t1 MTR_7g091370 49.123 114 56 2 2 114 1 113 4.39e-31 118
MS.gene62673.t1 MTR_1g100777 57.447 94 39 1 21 114 17 109 2.22e-30 118
MS.gene62673.t1 MTR_4g088900 40.645 155 82 5 21 166 32 185 1.78e-26 105
MS.gene62673.t1 MTR_5g047190 53.684 95 36 3 30 117 1 94 1.63e-21 88.6
MS.gene62673.t1 MTR_1g061170 35.928 167 98 3 83 248 14 172 5.26e-21 93.6
MS.gene62673.t1 MTR_4g045757 53.623 69 31 1 12 80 4 71 8.50e-15 69.3
MS.gene62673.t1 MTR_6g044340 32.857 140 82 4 75 214 14 141 1.27e-11 64.7
MS.gene62673.t1 MTR_2g043940 29.677 155 86 2 75 219 14 155 2.87e-11 64.7
MS.gene62673.t1 MTR_4g077970 33.333 105 63 2 160 257 2 106 7.92e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62673.t1 AT1G67970 43.978 357 159 6 20 360 17 348 6.41e-90 275
MS.gene62673.t1 AT1G32330 46.400 250 122 4 17 255 32 280 2.24e-63 210
MS.gene62673.t1 AT1G32330 46.400 250 122 4 17 255 32 280 2.85e-63 210
MS.gene62673.t1 AT5G16820 46.281 242 120 4 19 252 24 263 8.90e-60 201
MS.gene62673.t1 AT5G16820 46.281 242 120 4 19 252 24 263 8.90e-60 201
MS.gene62673.t1 AT4G17750 46.748 246 111 5 22 248 52 296 2.39e-59 200
MS.gene62673.t1 AT3G22830 48.768 203 102 2 22 223 61 262 1.05e-55 188
MS.gene62673.t1 AT2G26150 49.206 189 89 2 22 210 44 225 1.05e-55 186
MS.gene62673.t1 AT2G26150 49.206 189 89 2 22 210 44 225 1.05e-55 186
MS.gene62673.t1 AT3G02990 45.339 236 98 5 2 223 4 222 2.92e-55 188
MS.gene62673.t1 AT3G51910 43.814 194 87 4 22 215 29 200 9.01e-50 168
MS.gene62673.t1 AT5G03720 43.455 191 99 3 20 210 53 234 3.77e-47 166
MS.gene62673.t1 AT5G03720 43.455 191 99 3 20 210 53 234 3.77e-47 166
MS.gene62673.t1 AT5G43840 44.211 190 85 2 21 210 18 186 2.10e-46 160
MS.gene62673.t1 AT3G24520 43.939 198 81 3 20 217 15 182 3.31e-46 161
MS.gene62673.t1 AT4G13980 43.204 206 97 5 11 207 3 197 6.30e-45 160
MS.gene62673.t1 AT5G45710 41.706 211 108 3 16 222 7 206 3.87e-43 153
MS.gene62673.t1 AT5G45710 41.706 211 108 3 16 222 7 206 3.87e-43 153
MS.gene62673.t1 AT3G63350 40.284 211 111 4 21 231 27 222 8.37e-43 150
MS.gene62673.t1 AT5G54070 40.404 198 102 2 18 215 67 248 1.70e-42 151
MS.gene62673.t1 AT4G18880 44.670 197 103 2 14 210 7 197 2.53e-40 147
MS.gene62673.t1 AT1G46264 55.752 113 49 1 14 126 26 137 1.25e-36 136
MS.gene62673.t1 AT4G11660 59.574 94 37 1 21 114 58 150 5.05e-32 124
MS.gene62673.t1 AT5G62020 55.769 104 45 1 11 114 12 114 2.67e-31 120
MS.gene62673.t1 AT4G36990 57.447 94 39 1 21 114 13 105 2.99e-31 120
MS.gene62673.t1 AT2G41690 56.250 96 41 1 22 117 40 134 6.94e-31 118
MS.gene62673.t1 AT2G26150 42.308 130 69 1 81 210 47 170 5.22e-23 97.8
MS.gene62673.t1 AT2G26150 42.308 130 69 1 81 210 47 170 5.22e-23 97.8
MS.gene62673.t1 AT4G18870 52.174 92 42 2 19 110 10 99 2.60e-20 90.5
MS.gene62673.t1 AT4G18870 42.276 123 61 3 19 139 143 257 3.29e-20 90.1
MS.gene62673.t1 AT4G19630 36.842 95 56 2 19 110 8 101 1.49e-12 64.7
MS.gene62673.t1 AT1G77570 40.659 91 50 3 23 110 27 116 1.04e-11 62.8

Find 81 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATTGTGTTTGAAATAAT+TGG 0.094053 4.2:+1230077 None:intergenic
GTTGAATTTATCAGGGTTTC+AGG 0.157083 4.2:-1229244 MS.gene62673:intron
GGTGGTTCTGGTTCTGGTTC+TGG 0.187158 4.2:-1230237 MS.gene62673:CDS
AGGTTCTGGTTGCTTATTAA+AGG 0.234573 4.2:+1228689 None:intergenic
TTGCAAATGAAAACTTCATT+AGG 0.259058 4.2:-1229196 MS.gene62673:CDS
GGTTCATCGCGATTGTCTTT+TGG 0.270022 4.2:+1229095 None:intergenic
TGCAAATGAAAACTTCATTA+GGG 0.270385 4.2:-1229195 MS.gene62673:CDS
GAGAAGGGTGGTTCTGGTTC+TGG 0.286157 4.2:-1230243 MS.gene62673:CDS
CTTGAATAAATGTTACGATA+TGG 0.302015 4.2:-1230199 MS.gene62673:CDS
CGTGCAATGCCCTGGATTCT+TGG 0.305963 4.2:-1228877 MS.gene62673:CDS
TTATTGTGTTTGAAATAATT+GGG 0.306935 4.2:+1230078 None:intergenic
GCAGCAGATGCTCTCATTCT+TGG 0.308364 4.2:-1228907 MS.gene62673:CDS
TTCACCCTAAGGAAAATAAT+TGG 0.343688 4.2:-1228845 MS.gene62673:CDS
GTTTCTTGTACCTGATTTAC+CGG 0.350562 4.2:-1228568 MS.gene62673:CDS
AACGGAGAATGATTATCTAA+TGG 0.359885 4.2:+1228588 None:intergenic
GCGAGCGAGAAGGGTGGTTC+TGG 0.371236 4.2:-1230249 MS.gene62673:CDS
TGAAGCGATGATTATGGAAA+TGG 0.375364 4.2:-1228400 MS.gene62673:CDS
TTCTGGTTCTGGTTCTGGTT+CGG 0.392304 4.2:-1230232 MS.gene62673:CDS
CAACCGATTCCATCATCTCT+TGG 0.401097 4.2:-1230164 MS.gene62673:CDS
CTACTGAGTGATCGCCTTGA+AGG 0.403981 4.2:-1228945 MS.gene62673:CDS
GATGCTTCTCCATTCCTTCA+AGG 0.410224 4.2:+1228931 None:intergenic
TGATTTACTGCTTCTTGTGA+TGG 0.416635 4.2:+1229074 None:intergenic
GGAAAATTGATGCAGATCAC+TGG 0.420160 4.2:-1229223 MS.gene62673:CDS
TGCTGAAGAATATACGTAGA+AGG 0.420862 4.2:-1229160 MS.gene62673:CDS
GTCTGTCATCTTGGTTACCT+TGG 0.422781 4.2:+1228793 None:intergenic
GAACCGTCATCCGGTAAATC+AGG 0.426775 4.2:+1228558 None:intergenic
ACGGAGAATGATTATCTAAT+GGG 0.429612 4.2:+1228589 None:intergenic
GCAGGAGATCGTGGAACAAC+AGG 0.434299 4.2:+1228711 None:intergenic
TCTGTAGAAACCTCAGGTTC+TGG 0.439677 4.2:+1228675 None:intergenic
AAGCAGTAAATCATGGCCTT+TGG 0.439818 4.2:-1229064 MS.gene62673:CDS
ATCATCCCATCAGAAGAAAC+TGG 0.445617 4.2:+1228771 None:intergenic
AAGAAACTGGTCTGTCATCT+TGG 0.448044 4.2:+1228784 None:intergenic
AGAAGTTGAGAATCTAAAAT+CGG 0.450572 4.2:-1229039 MS.gene62673:CDS
GAACCTGAGGTTTCTACAGA+TGG 0.463364 4.2:-1228672 MS.gene62673:CDS
AATTGGCGTTTCTCTGAAGC+AGG 0.464691 4.2:-1228828 MS.gene62673:CDS
GCAGCAACAGCGAGCGAGAA+GGG 0.473861 4.2:-1230258 MS.gene62673:CDS
AACCCATCTGTAGAAACCTC+AGG 0.474952 4.2:+1228669 None:intergenic
TGTTCCCACGAACCGTCATC+CGG 0.475845 4.2:+1228549 None:intergenic
GGTTCAGCTGCTTCACCCTA+AGG 0.476383 4.2:-1228856 MS.gene62673:CDS
TCGTAACATTTATTCAAGAA+AGG 0.477793 4.2:+1230204 None:intergenic
GCTTACTCTCAGAAGATTCA+AGG 0.482301 4.2:+1228973 None:intergenic
AGCAGCTGAACCAAGAATCC+AGG 0.483387 4.2:+1228867 None:intergenic
ATTTACCGGATGACGGTTCG+TGG 0.488372 4.2:-1228554 MS.gene62673:CDS
GAGTGATCGCCTTGAAGGAA+TGG 0.496645 4.2:-1228940 MS.gene62673:CDS
TCTGGTTGCTTATTAAAGGC+AGG 0.497515 4.2:+1228693 None:intergenic
GTCATGGTCGTGCAATGCCC+TGG 0.501216 4.2:-1228885 MS.gene62673:CDS
GACAGACCAGTTTCTTCTGA+TGG 0.504911 4.2:-1228777 MS.gene62673:CDS
TCTCTGAAGCAGGGAATATG+TGG 0.508698 4.2:-1228818 MS.gene62673:CDS
GATTCTCAACTTCTCTCCAA+AGG 0.510423 4.2:+1229048 None:intergenic
TATTGTGTTTGAAATAATTG+GGG 0.510469 4.2:+1230079 None:intergenic
GATGCTCTCATTCTTGGTCA+TGG 0.526629 4.2:-1228901 MS.gene62673:CDS
GTTGAGTTGTCTGACGAAGC+TGG 0.529603 4.2:+1230050 None:intergenic
GAGATGATGGAATCGGTTGA+AGG 0.531686 4.2:+1230168 None:intergenic
ATCATCTCTTGGTCCGCTGA+TGG 0.533246 4.2:-1230153 MS.gene62673:CDS
ATCAGCGGACCAAGAGATGA+TGG 0.535757 4.2:+1230155 None:intergenic
GGTAAATCAGGTACAAGAAA+CGG 0.535891 4.2:+1228570 None:intergenic
TGGTTCTGGTTCTGGTTCGG+TGG 0.539717 4.2:-1230229 MS.gene62673:CDS
GAGTGAGAATTGATCAGCGT+TGG 0.547894 4.2:+1230110 None:intergenic
GTACCTGATTTACCGGATGA+CGG 0.554758 4.2:-1228561 MS.gene62673:CDS
AACTGTTAGTGACATGGAGA+TGG 0.556871 4.2:-1228463 MS.gene62673:CDS
ACAGACCAGTTTCTTCTGAT+GGG 0.557728 4.2:-1228776 MS.gene62673:CDS
GTTTGACCAACTCCTGCGTG+AGG 0.558206 4.2:+1229006 None:intergenic
GGACCAAGAGATGATGGAAT+CGG 0.561010 4.2:+1230161 None:intergenic
TTTACCGGATGACGGTTCGT+GGG 0.565563 4.2:-1228553 MS.gene62673:CDS
AGCAGCAACAGCGAGCGAGA+AGG 0.566061 4.2:-1230259 MS.gene62673:CDS
TCACAAGAAGCAGTAAATCA+TGG 0.568437 4.2:-1229071 MS.gene62673:CDS
TGCTGATCTGCAGCAACATG+TGG 0.573167 4.2:+1229131 None:intergenic
AACCTGAGGTTTCTACAGAT+GGG 0.578276 4.2:-1228671 MS.gene62673:CDS
TTTGACCAACTCCTGCGTGA+GGG 0.583918 4.2:+1229007 None:intergenic
GAAAATTGATGCAGATCACT+GGG 0.584009 4.2:-1229222 MS.gene62673:CDS
GCAAATGAAAACTTCATTAG+GGG 0.585563 4.2:-1229194 MS.gene62673:CDS
TAATAAGCAACCAGAACCTG+AGG 0.601561 4.2:-1228685 MS.gene62673:CDS
GCAGCTGAACCAAGAATCCA+GGG 0.614903 4.2:+1228868 None:intergenic
TTATTAAAGGCAGGAGATCG+TGG 0.635093 4.2:+1228702 None:intergenic
CTCTGAAGCAGGGAATATGT+GGG 0.641241 4.2:-1228817 MS.gene62673:CDS
ATTGGCGTTTCTCTGAAGCA+GGG 0.648668 4.2:-1228827 MS.gene62673:CDS
GGAGCAAACTGTTAGTGACA+TGG 0.649582 4.2:-1228469 MS.gene62673:CDS
GCAGGGAATATGTGGGACCA+AGG 0.652641 4.2:-1228810 MS.gene62673:CDS
GCAACAGCGAGCGAGAAGGG+TGG 0.652707 4.2:-1230255 MS.gene62673:CDS
GAAACTATTGTTACCATCAG+CGG 0.656676 4.2:+1230140 None:intergenic
AGATACTGATCGTGAAAACG+AGG 0.683103 4.2:-1228490 MS.gene62673:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATATGAATTAATAATTGA+AGG + chr4.2:1228652-1228671 None:intergenic 15.0%
!! CATATGAATTAATAATTGAA+GGG + chr4.2:1228651-1228670 None:intergenic 15.0%
!! TAGATAAACAAAATTATTGT+AGG + chr4.2:1229320-1229339 None:intergenic 15.0%
!!! ATTATATTATTAATGGGTAA+AGG - chr4.2:1228751-1228770 MS.gene62673:CDS 15.0%
!!! ATTATTGTGTTTGAAATAAT+TGG + chr4.2:1228576-1228595 None:intergenic 15.0%
!!! TACAATAATTTTGTTTATCT+AGG - chr4.2:1229319-1229338 MS.gene62673:intron 15.0%
!!! TTATTGTGTTTGAAATAATT+GGG + chr4.2:1228575-1228594 None:intergenic 15.0%
!! ATTTAAACACAAACTGATAT+AGG + chr4.2:1228721-1228740 None:intergenic 20.0%
!! TTATACCTACATATAAATCA+AGG + chr4.2:1228787-1228806 None:intergenic 20.0%
!! TTTAAACACAAACTGATATA+GGG + chr4.2:1228720-1228739 None:intergenic 20.0%
!!! ATAAAATAGTCAAAGTGTTT+TGG + chr4.2:1229098-1229117 None:intergenic 20.0%
!!! TATTGTGTTTGAAATAATTG+GGG + chr4.2:1228574-1228593 None:intergenic 20.0%
! AAAATCCATTAGTAGTACTT+AGG - chr4.2:1228805-1228824 MS.gene62673:CDS 25.0%
! AAATCCATTAGTAGTACTTA+GGG - chr4.2:1228806-1228825 MS.gene62673:CDS 25.0%
! CTTGAATAAATGTTACGATA+TGG - chr4.2:1228451-1228470 MS.gene62673:CDS 25.0%
! TATGGATAAACAAGTTAATC+AGG + chr4.2:1229183-1229202 None:intergenic 25.0%
! TCGTAACATTTATTCAAGAA+AGG + chr4.2:1228449-1228468 None:intergenic 25.0%
! TGCAAATGAAAACTTCATTA+GGG - chr4.2:1229455-1229474 MS.gene62673:intron 25.0%
! TGTTTCCTTGATTTATATGT+AGG - chr4.2:1228779-1228798 MS.gene62673:CDS 25.0%
! TTGCAAATGAAAACTTCATT+AGG - chr4.2:1229454-1229473 MS.gene62673:intron 25.0%
!! AGAAGTTGAGAATCTAAAAT+CGG - chr4.2:1229611-1229630 MS.gene62673:intron 25.0%
!!! CAGTATCTTCAACATTTTTT+AGG + chr4.2:1230148-1230167 None:intergenic 25.0%
!!! TCTTCAACATTTTTTAGGAT+AGG + chr4.2:1230143-1230162 None:intergenic 25.0%
AAACTGATATAGGGTTAATG+AGG + chr4.2:1228711-1228730 None:intergenic 30.0%
AACGGAGAATGATTATCTAA+TGG + chr4.2:1230065-1230084 None:intergenic 30.0%
ACACTTTAAACAAATCGAGA+CGG + chr4.2:1229357-1229376 None:intergenic 30.0%
ACGGAGAATGATTATCTAAT+GGG + chr4.2:1230064-1230083 None:intergenic 30.0%
GATGACTGTTGAATTTATCA+GGG - chr4.2:1229399-1229418 MS.gene62673:intron 30.0%
GCAAATGAAAACTTCATTAG+GGG - chr4.2:1229456-1229475 MS.gene62673:intron 30.0%
TGATGACTGTTGAATTTATC+AGG - chr4.2:1229398-1229417 MS.gene62673:intron 30.0%
TGTAGTAAAGGTTTCTCAAA+AGG + chr4.2:1229263-1229282 None:intergenic 30.0%
TTCACCCTAAGGAAAATAAT+TGG - chr4.2:1229805-1229824 MS.gene62673:intron 30.0%
! AAACGCCAATTATTTTCCTT+AGG + chr4.2:1229813-1229832 None:intergenic 30.0%
! AACGCCAATTATTTTCCTTA+GGG + chr4.2:1229812-1229831 None:intergenic 30.0%
!!! TGATTTTGAAGCGATGATTA+TGG - chr4.2:1230244-1230263 MS.gene62673:CDS 30.0%
!!! TTACTTCTACGACACTTTTT+TGG - chr4.2:1228837-1228856 MS.gene62673:CDS 30.0%
AACAGTGGTCTGTTCAAATT+CGG + chr4.2:1228923-1228942 None:intergenic 35.0%
CAAACCCTAAGTACTACTAA+TGG + chr4.2:1228813-1228832 None:intergenic 35.0%
GAAAATTGATGCAGATCACT+GGG - chr4.2:1229428-1229447 MS.gene62673:intron 35.0%
GAAACTATTGTTACCATCAG+CGG + chr4.2:1228513-1228532 None:intergenic 35.0%
GGTAAATCAGGTACAAGAAA+CGG + chr4.2:1230083-1230102 None:intergenic 35.0%
GTTGAATTTATCAGGGTTTC+AGG - chr4.2:1229406-1229425 MS.gene62673:intron 35.0%
GTTTCTTGTACCTGATTTAC+CGG - chr4.2:1230082-1230101 MS.gene62673:CDS 35.0%
TCACAAGAAGCAGTAAATCA+TGG - chr4.2:1229579-1229598 MS.gene62673:intron 35.0%
TGAAGCGATGATTATGGAAA+TGG - chr4.2:1230250-1230269 MS.gene62673:CDS 35.0%
TGATATAGGGTTAATGAGGT+CGG + chr4.2:1228707-1228726 None:intergenic 35.0%
TGATTTACTGCTTCTTGTGA+TGG + chr4.2:1229579-1229598 None:intergenic 35.0%
TGCTGAAGAATATACGTAGA+AGG - chr4.2:1229490-1229509 MS.gene62673:intron 35.0%
TTTCAATTCAGCAGCATCAA+AGG + chr4.2:1229218-1229237 None:intergenic 35.0%
! AGGTTCTGGTTGCTTATTAA+AGG + chr4.2:1229964-1229983 None:intergenic 35.0%
!! ACTATTTCTAGAAGCTATCC+TGG + chr4.2:1229063-1229082 None:intergenic 35.0%
!! TCGTGGGAACAACTATTTTT+AGG - chr4.2:1230113-1230132 MS.gene62673:CDS 35.0%
!!! GGTTTTAGTTTTTCAGCAAC+AGG + chr4.2:1229921-1229940 None:intergenic 35.0%
!!! TTTAGTTTTTCAGCAACAGG+TGG + chr4.2:1229918-1229937 None:intergenic 35.0%
AACCTGAGGTTTCTACAGAT+GGG - chr4.2:1229979-1229998 MS.gene62673:intron 40.0%
AACTGTTAGTGACATGGAGA+TGG - chr4.2:1230187-1230206 MS.gene62673:CDS 40.0%
AAGCAGTAAATCATGGCCTT+TGG - chr4.2:1229586-1229605 MS.gene62673:intron 40.0%
AATCGAGACGGTAAAAATGC+AGG + chr4.2:1229345-1229364 None:intergenic 40.0%
ACAGACCAGTTTCTTCTGAT+GGG - chr4.2:1229874-1229893 MS.gene62673:intron 40.0%
AGATACTGATCGTGAAAACG+AGG - chr4.2:1230160-1230179 MS.gene62673:CDS 40.0%
ATCATCCCATCAGAAGAAAC+TGG + chr4.2:1229882-1229901 None:intergenic 40.0%
CAAAGGATTACGGTTCTCTA+TGG + chr4.2:1229201-1229220 None:intergenic 40.0%
CTTACAGTCATTGACAATCG+CGG + chr4.2:1229017-1229036 None:intergenic 40.0%
GATTCTCAACTTCTCTCCAA+AGG + chr4.2:1229605-1229624 None:intergenic 40.0%
GCTTACTCTCAGAAGATTCA+AGG + chr4.2:1229680-1229699 None:intergenic 40.0%
GGAAAATTGATGCAGATCAC+TGG - chr4.2:1229427-1229446 MS.gene62673:intron 40.0%
GGTTAATGAGGTCGGTAATT+GGG + chr4.2:1228699-1228718 None:intergenic 40.0%
GTTAATGAGGTCGGTAATTG+GGG + chr4.2:1228698-1228717 None:intergenic 40.0%
GTTGCATGTTGCTGTAGTAA+AGG + chr4.2:1229275-1229294 None:intergenic 40.0%
TAATAAGCAACCAGAACCTG+AGG - chr4.2:1229965-1229984 MS.gene62673:intron 40.0%
TCAGCAGCATCAAAGGATTA+CGG + chr4.2:1229211-1229230 None:intergenic 40.0%
TCATTGACAATCGCGGAAAA+TGG + chr4.2:1229010-1229029 None:intergenic 40.0%
TCTAGAAGCTATCCTGGTTA+CGG + chr4.2:1229057-1229076 None:intergenic 40.0%
TCTGGTTGCTTATTAAAGGC+AGG + chr4.2:1229960-1229979 None:intergenic 40.0%
TGTAGGTTCTTCTACTGAGT+GGG + chr4.2:1229303-1229322 None:intergenic 40.0%
TTAATGAGGTCGGTAATTGG+GGG + chr4.2:1228697-1228716 None:intergenic 40.0%
TTATTAAAGGCAGGAGATCG+TGG + chr4.2:1229951-1229970 None:intergenic 40.0%
TTGTAGGTTCTTCTACTGAG+TGG + chr4.2:1229304-1229323 None:intergenic 40.0%
! AAGAAACTGGTCTGTCATCT+TGG + chr4.2:1229869-1229888 None:intergenic 40.0%
AACCCATCTGTAGAAACCTC+AGG + chr4.2:1229984-1230003 None:intergenic 45.0%
AGTGTATCGCAGAACAACAG+TGG + chr4.2:1228938-1228957 None:intergenic 45.0%
GAACCTGAGGTTTCTACAGA+TGG - chr4.2:1229978-1229997 MS.gene62673:intron 45.0%
GACAGACCAGTTTCTTCTGA+TGG - chr4.2:1229873-1229892 MS.gene62673:intron 45.0%
GAGTGAGAATTGATCAGCGT+TGG + chr4.2:1228543-1228562 None:intergenic 45.0%
GATGCTCTCATTCTTGGTCA+TGG - chr4.2:1229749-1229768 MS.gene62673:intron 45.0%
GATGCTTCTCCATTCCTTCA+AGG + chr4.2:1229722-1229741 None:intergenic 45.0%
GGAGCAAACTGTTAGTGACA+TGG - chr4.2:1230181-1230200 MS.gene62673:CDS 45.0%
GGGTTAATGAGGTCGGTAAT+TGG + chr4.2:1228700-1228719 None:intergenic 45.0%
GTACCTGATTTACCGGATGA+CGG - chr4.2:1230089-1230108 MS.gene62673:CDS 45.0%
GTCTGTCATCTTGGTTACCT+TGG + chr4.2:1229860-1229879 None:intergenic 45.0%
TCTGTAGAAACCTCAGGTTC+TGG + chr4.2:1229978-1229997 None:intergenic 45.0%
TTCAGCTTACTTCCGTAACC+AGG - chr4.2:1229042-1229061 MS.gene62673:CDS 45.0%
TTGACAATCGCGGAAAATGG+CGG + chr4.2:1229007-1229026 None:intergenic 45.0%
! CAACCGATTCCATCATCTCT+TGG - chr4.2:1228486-1228505 MS.gene62673:CDS 45.0%
! GGTTCATCGCGATTGTCTTT+TGG + chr4.2:1229558-1229577 None:intergenic 45.0%
! TCATCGCGATTGTCTTTTGG+CGG + chr4.2:1229555-1229574 None:intergenic 45.0%
!! AATTGGCGTTTCTCTGAAGC+AGG - chr4.2:1229822-1229841 MS.gene62673:intron 45.0%
!! ATTGGCGTTTCTCTGAAGCA+GGG - chr4.2:1229823-1229842 MS.gene62673:intron 45.0%
!! CTCTGAAGCAGGGAATATGT+GGG - chr4.2:1229833-1229852 MS.gene62673:intron 45.0%
!! GAGATGATGGAATCGGTTGA+AGG + chr4.2:1228485-1228504 None:intergenic 45.0%
!! GGACCAAGAGATGATGGAAT+CGG + chr4.2:1228492-1228511 None:intergenic 45.0%
!! TCTCTGAAGCAGGGAATATG+TGG - chr4.2:1229832-1229851 MS.gene62673:intron 45.0%
!! TTCTGGTTCTGGTTCTGGTT+CGG - chr4.2:1228418-1228437 MS.gene62673:CDS 45.0%
!! ATACTATATTATATTATTAA+TGG - chr4.2:1228744-1228763 MS.gene62673:CDS 5.0%
!! TACTATATTATATTATTAAT+GGG - chr4.2:1228745-1228764 MS.gene62673:CDS 5.0%
AAAAACCCTCACGCAGGAGT+TGG - chr4.2:1229638-1229657 MS.gene62673:intron 50.0%
AGCAGCTGAACCAAGAATCC+AGG + chr4.2:1229786-1229805 None:intergenic 50.0%
ATCAGCGGACCAAGAGATGA+TGG + chr4.2:1228498-1228517 None:intergenic 50.0%
ATCATCTCTTGGTCCGCTGA+TGG - chr4.2:1228497-1228516 MS.gene62673:CDS 50.0%
CTACTGAGTGATCGCCTTGA+AGG - chr4.2:1229705-1229724 MS.gene62673:intron 50.0%
GAACCGTCATCCGGTAAATC+AGG + chr4.2:1230095-1230114 None:intergenic 50.0%
GAGTGATCGCCTTGAAGGAA+TGG - chr4.2:1229710-1229729 MS.gene62673:intron 50.0%
GCAGCAGATGCTCTCATTCT+TGG - chr4.2:1229743-1229762 MS.gene62673:intron 50.0%
GCAGCTGAACCAAGAATCCA+GGG + chr4.2:1229785-1229804 None:intergenic 50.0%
GGATAGAAAAACCCTCACGC+AGG - chr4.2:1229632-1229651 MS.gene62673:intron 50.0%
TCGGCATAAGCTCTCCCAAA+CGG + chr4.2:1228904-1228923 None:intergenic 50.0%
TCTCCCAAACGGTCTCAGAA+GGG + chr4.2:1228893-1228912 None:intergenic 50.0%
TGCTGATCTGCAGCAACATG+TGG + chr4.2:1229522-1229541 None:intergenic 50.0%
TTTGACCAACTCCTGCGTGA+GGG + chr4.2:1229646-1229665 None:intergenic 50.0%
! ATTTACCGGATGACGGTTCG+TGG - chr4.2:1230096-1230115 MS.gene62673:CDS 50.0%
! CGATTGTCTTTTGGCGGCAA+TGG + chr4.2:1229549-1229568 None:intergenic 50.0%
! GTTGAGTTGTCTGACGAAGC+TGG + chr4.2:1228603-1228622 None:intergenic 50.0%
! TTTACCGGATGACGGTTCGT+GGG - chr4.2:1230097-1230116 MS.gene62673:CDS 50.0%
!! AAGCCCTTCTGAGACCGTTT+GGG - chr4.2:1228887-1228906 MS.gene62673:CDS 50.0%
!! TAAGCCCTTCTGAGACCGTT+TGG - chr4.2:1228886-1228905 MS.gene62673:CDS 50.0%
CGTGCAATGCCCTGGATTCT+TGG - chr4.2:1229773-1229792 MS.gene62673:intron 55.0%
CTCTCCCAAACGGTCTCAGA+AGG + chr4.2:1228894-1228913 None:intergenic 55.0%
GAGGTCGGTAATTGGGGGAA+AGG + chr4.2:1228692-1228711 None:intergenic 55.0%
GCAGGAGATCGTGGAACAAC+AGG + chr4.2:1229942-1229961 None:intergenic 55.0%
GCAGGGAATATGTGGGACCA+AGG - chr4.2:1229840-1229859 MS.gene62673:intron 55.0%
GGTTCAGCTGCTTCACCCTA+AGG - chr4.2:1229794-1229813 MS.gene62673:intron 55.0%
GTTTGACCAACTCCTGCGTG+AGG + chr4.2:1229647-1229666 None:intergenic 55.0%
TGTTCCCACGAACCGTCATC+CGG + chr4.2:1230104-1230123 None:intergenic 55.0%
!! GAGAAGGGTGGTTCTGGTTC+TGG - chr4.2:1228407-1228426 MS.gene62673:CDS 55.0%
!! GGTGGTTCTGGTTCTGGTTC+TGG - chr4.2:1228413-1228432 MS.gene62673:CDS 55.0%
!! TGGTTCTGGTTCTGGTTCGG+TGG - chr4.2:1228421-1228440 MS.gene62673:CDS 55.0%
AGCAGCAACAGCGAGCGAGA+AGG - chr4.2:1228391-1228410 MS.gene62673:CDS 60.0%
GCAGCAACAGCGAGCGAGAA+GGG - chr4.2:1228392-1228411 MS.gene62673:CDS 60.0%
GTCATGGTCGTGCAATGCCC+TGG - chr4.2:1229765-1229784 MS.gene62673:intron 60.0%
GCAACAGCGAGCGAGAAGGG+TGG - chr4.2:1228395-1228414 MS.gene62673:CDS 65.0%
GCGAGCGAGAAGGGTGGTTC+TGG - chr4.2:1228401-1228420 MS.gene62673:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 1228383 1230289 1228383 ID=MS.gene62673
chr4.2 mRNA 1228383 1230289 1228383 ID=MS.gene62673.t1;Parent=MS.gene62673
chr4.2 exon 1230044 1230289 1230044 ID=MS.gene62673.t1.exon1;Parent=MS.gene62673.t1
chr4.2 CDS 1230044 1230289 1230044 ID=cds.MS.gene62673.t1;Parent=MS.gene62673.t1
chr4.2 exon 1228383 1229252 1228383 ID=MS.gene62673.t1.exon2;Parent=MS.gene62673.t1
chr4.2 CDS 1228383 1229252 1228383 ID=cds.MS.gene62673.t1;Parent=MS.gene62673.t1
Gene Sequence

>MS.gene62673

ATGGTGAAAGCAGCAACAGCGAGCGAGAAGGGTGGTTCTGGTTCTGGTTCTGGTTCGGTGGCACCTTTCTTGAATAAATGTTACGATATGGTTGAAGATCCTTCAACCGATTCCATCATCTCTTGGTCCGCTGATGGTAACAATAGTTTCATCATCTCCAACGCTGATCAATTCTCACTCACTCTTCTCCCCAATTATTTCAAACACAATAATTTCTCCAGCTTCGTCAGACAACTCAACATCTATGTATGCTCTTCTTATTCATCCCTTCAATTATTAATTCATATGTTCATTAATTTCTCTGTTCCTTTCCCCCAATTACCGACCTCATTAACCCTATATCAGTTTGTGTTTAAATACTATACTATATTATATTATTAATGGGTAAAGGTATTCTGTTTCCTTGATTTATATGTAGGTATAAAATCCATTAGTAGTACTTAGGGTTTGCATTTTACTTCTACGACACTTTTTTGGATTATTTGAGTTTATATGTTGACATATAAGCCCTTCTGAGACCGTTTGGGAGAGCTTATGCCGAATTTGAACAGACCACTGTTGTTCTGCGATACACTATTTAGTACATACTGCTGTCAAATAACAGCTATAGAACTTGAACAACCGCCATTTTCCGCGATTGTCAATGACTGTAAGCTGTTTTCAGCTTACTTCCGTAACCAGGATAGCTTCTAGAAATAGTTTATAGTTTATACCAAAACACTTTGACTATTTTATTTTTTGTTTTAGAGATAGCTTATATATAAACTGTTATCTTACAAGTGTTTATGCTATAAGTGCCTGATTAACTTGTTTATCCATAGAGAACCGTAATCCTTTGATGCTGCTGAATTGAAAATGTATTAGATAATATACTCGACCTTTTGAGAAACCTTTACTACAGCAACATGCAACGTTTTCCCACTCAGTAGAAGAACCTACAATAATTTTGTTTATCTAGGCCTGCATTTTTACCGTCTCGATTTGTTTAAAGTGTCTCTCTTTTAAAGTCAGCAACTGATGACTGTTGAATTTATCAGGGTTTCAGGAAAATTGATGCAGATCACTGGGAATTTGCAAATGAAAACTTCATTAGGGGTCAAAAGCATTTGCTGAAGAATATACGTAGAAGGAAACATCCACATGTTGCTGCAGATCAGCAAAAACCATTGCCGCCAAAAGACAATCGCGATGAACCATCACAAGAAGCAGTAAATCATGGCCTTTGGAGAGAAGTTGAGAATCTAAAATCGGATAGAAAAACCCTCACGCAGGAGTTGGTCAAACATAAGCAGCACCTTGAATCTTCTGAGAGTAAGCTGCTTCTACTGAGTGATCGCCTTGAAGGAATGGAGAAGCATCAGCAGCAGATGCTCTCATTCTTGGTCATGGTCGTGCAATGCCCTGGATTCTTGGTTCAGCTGCTTCACCCTAAGGAAAATAATTGGCGTTTCTCTGAAGCAGGGAATATGTGGGACCAAGGTAACCAAGATGACAGACCAGTTTCTTCTGATGGGATGATTATAAAGTATACACCACCTGTTGCTGAAAAACTAAAACCTGTTGTTCCACGATCTCCTGCCTTTAATAAGCAACCAGAACCTGAGGTTTCTACAGATGGGTTAAAAGACTTGTGCATCAGTTCTGAGTTCTTGAAATTGCTTCTAGATGAGAAATTGTGCCCATTAGATAATCATTCTCCGTTTCTTGTACCTGATTTACCGGATGACGGTTCGTGGGAACAACTATTTTTAGGCAGTCCTATCCTAAAAAATGTTGAAGATACTGATCGTGAAAACGAGGAGCAAACTGTTAGTGACATGGAGATGGATCACATCATATCTGAAGCTCTAAATGAAACATCTGATTTTGAAGCGATGATTATGGAAATGGAGAACACTCACGCATGA
Protein sequence

>MS.gene62673.t1

MVKAATASEKGGSGSGSGSVAPFLNKCYDMVEDPSTDSIISWSADGNNSFIISNADQFSLTLLPNYFKHNNFSSFVRQLNIYGFRKIDADHWEFANENFIRGQKHLLKNIRRRKHPHVAADQQKPLPPKDNRDEPSQEAVNHGLWREVENLKSDRKTLTQELVKHKQHLESSESKLLLLSDRLEGMEKHQQQMLSFLVMVVQCPGFLVQLLHPKENNWRFSEAGNMWDQGNQDDRPVSSDGMIIKYTPPVAEKLKPVVPRSPAFNKQPEPEVSTDGLKDLCISSEFLKLLLDEKLCPLDNHSPFLVPDLPDDGSWEQLFLGSPILKNVEDTDRENEEQTVSDMEMDHIISEALNETSDFEAMIMEMENTHA