Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62688.t1 | XP_024641592.1 | 59.8 | 97 | 27 | 1 | 1 | 97 | 39 | 123 | 7.90E-23 | 116.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058625 | MS.gene62688 | 0.80014 | 1.66E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGTTCTAGCAACTTGAAT+TGG | 0.324721 | 4.2:-1532726 | None:intergenic |
| TGCACTAACTCAGCATGTTC+AGG | 0.335036 | 4.2:-1532786 | None:intergenic |
| GCAACTTGAATTGGAATTTG+AGG | 0.378140 | 4.2:-1532717 | None:intergenic |
| ATATGTTTAAGCATGTTGTT+AGG | 0.426589 | 4.2:-1532936 | None:intergenic |
| ACTCAGCATGTTCAGGTCCT+TGG | 0.469300 | 4.2:-1532779 | None:intergenic |
| TCTGTTATGTCAAATAGTTA+TGG | 0.477288 | 4.2:+1532906 | MS.gene62688:CDS |
| CTAGACATCCTTCAATGGCT+TGG | 0.481979 | 4.2:+1532832 | MS.gene62688:CDS |
| AAATGTCTCCAAGCCATTGA+AGG | 0.489069 | 4.2:-1532840 | None:intergenic |
| AGTGAAACAGAAGTTACAAA+AGG | 0.500447 | 4.2:-1532883 | None:intergenic |
| AGATGCTAGACATCCTTCAA+TGG | 0.529205 | 4.2:+1532827 | MS.gene62688:CDS |
| TAAGCATGTTGTTAGGTGGA+TGG | 0.572076 | 4.2:-1532929 | None:intergenic |
| TGTTTAAGCATGTTGTTAGG+TGG | 0.597759 | 4.2:-1532933 | None:intergenic |
| TGCTAGAACACAAGAAGTTG+AGG | 0.602315 | 4.2:+1532737 | MS.gene62688:CDS |
| AGGAAAATGATTCACACTAG+TGG | 0.616118 | 4.2:-1532697 | None:intergenic |
| GCTGAGTTAGTACATAGCCA+AGG | 0.681772 | 4.2:+1532762 | MS.gene62688:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATATGTTTAAGCATGTTGTT+AGG | - | chr4.2:1532939-1532958 | None:intergenic | 25.0% |
| ! | TCTGTTATGTCAAATAGTTA+TGG | + | chr4.2:1532906-1532925 | MS.gene62688:CDS | 25.0% |
| AGTGAAACAGAAGTTACAAA+AGG | - | chr4.2:1532886-1532905 | None:intergenic | 30.0% | |
| AGGAAAATGATTCACACTAG+TGG | - | chr4.2:1532700-1532719 | None:intergenic | 35.0% | |
| GCAACTTGAATTGGAATTTG+AGG | - | chr4.2:1532720-1532739 | None:intergenic | 35.0% | |
| TGTGTTCTAGCAACTTGAAT+TGG | - | chr4.2:1532729-1532748 | None:intergenic | 35.0% | |
| TGTTTAAGCATGTTGTTAGG+TGG | - | chr4.2:1532936-1532955 | None:intergenic | 35.0% | |
| AAATGTCTCCAAGCCATTGA+AGG | - | chr4.2:1532843-1532862 | None:intergenic | 40.0% | |
| AGATGCTAGACATCCTTCAA+TGG | + | chr4.2:1532827-1532846 | MS.gene62688:CDS | 40.0% | |
| TAAGCATGTTGTTAGGTGGA+TGG | - | chr4.2:1532932-1532951 | None:intergenic | 40.0% | |
| TGCTAGAACACAAGAAGTTG+AGG | + | chr4.2:1532737-1532756 | MS.gene62688:CDS | 40.0% | |
| CTAGACATCCTTCAATGGCT+TGG | + | chr4.2:1532832-1532851 | MS.gene62688:CDS | 45.0% | |
| GCTGAGTTAGTACATAGCCA+AGG | + | chr4.2:1532762-1532781 | MS.gene62688:CDS | 45.0% | |
| TGCACTAACTCAGCATGTTC+AGG | - | chr4.2:1532789-1532808 | None:intergenic | 45.0% | |
| ACTCAGCATGTTCAGGTCCT+TGG | - | chr4.2:1532782-1532801 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 1532684 | 1532980 | 1532684 | ID=MS.gene62688 |
| chr4.2 | mRNA | 1532684 | 1532980 | 1532684 | ID=MS.gene62688.t1;Parent=MS.gene62688 |
| chr4.2 | exon | 1532684 | 1532980 | 1532684 | ID=MS.gene62688.t1.exon1;Parent=MS.gene62688.t1 |
| chr4.2 | CDS | 1532684 | 1532980 | 1532684 | ID=cds.MS.gene62688.t1;Parent=MS.gene62688.t1 |
| Gene Sequence |
| Protein sequence |
