AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62735

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62735.t1 MTR_7g029525 94.125 817 48 0 1 817 508 1324 0.0 1566
MS.gene62735.t1 MTR_7g450790 83.272 819 127 4 4 817 626 1439 0.0 1363
MS.gene62735.t1 MTR_7g450790 65.278 72 25 0 61 132 461 532 2.00e-20 97.8
MS.gene62735.t1 MTR_4g088650 79.659 821 133 4 2 817 427 1218 0.0 1288
MS.gene62735.t1 MTR_7g450780 67.571 848 231 14 2 817 511 1346 0.0 1065
MS.gene62735.t1 MTR_7g450780 64.384 73 26 0 61 133 387 459 2.34e-19 94.4
MS.gene62735.t1 MTR_7g450820 67.028 831 246 10 2 816 416 1234 0.0 1063
MS.gene62735.t1 MTR_7g050445 66.187 834 248 13 2 817 481 1298 0.0 1060
MS.gene62735.t1 MTR_7g450860 65.306 833 255 13 4 816 454 1272 0.0 1020
MS.gene62735.t1 MTR_2g084695 67.514 354 43 2 464 817 9 290 8.54e-151 443
MS.gene62735.t1 MTR_1g081750 32.986 767 440 18 76 798 363 1099 2.71e-117 382
MS.gene62735.t1 MTR_7g026650 31.865 772 463 17 82 817 408 1152 1.89e-110 365
MS.gene62735.t1 MTR_3g071860 32.922 729 431 17 111 798 539 1250 2.35e-108 360
MS.gene62735.t1 MTR_5g083300 30.702 798 493 17 43 798 508 1287 1.49e-104 350
MS.gene62735.t1 MTR_5g083300 30.702 798 493 17 43 798 545 1324 2.44e-104 350
MS.gene62735.t1 MTR_8g037345 29.063 726 427 18 111 810 234 897 4.30e-87 296
MS.gene62735.t1 MTR_2g437480 31.378 682 376 18 111 767 387 1001 9.23e-87 297
MS.gene62735.t1 MTR_7g026620 25.997 577 298 16 155 713 177 642 1.13e-45 174
MS.gene62735.t1 MTR_5g004720 24.493 592 351 15 216 771 161 692 1.05e-35 146
MS.gene62735.t1 MTR_8g094070 25.043 583 331 20 231 770 373 892 1.78e-28 123
MS.gene62735.t1 MTR_8g094070 25.043 583 331 20 231 770 373 892 1.83e-28 123
MS.gene62735.t1 MTR_1g069755 24.490 588 327 21 222 767 371 883 3.39e-27 119
MS.gene62735.t1 MTR_3g106210 23.921 556 343 13 278 802 309 815 5.23e-25 112
MS.gene62735.t1 MTR_3g106210 23.921 556 343 13 278 802 309 815 5.75e-25 112
MS.gene62735.t1 MTR_3g106210 23.921 556 343 13 278 802 309 815 6.34e-25 112
MS.gene62735.t1 MTR_3g106210 23.921 556 343 13 278 802 309 815 6.35e-25 112
MS.gene62735.t1 MTR_1g013190 23.211 629 392 19 205 794 228 804 5.03e-24 109
MS.gene62735.t1 MTR_1g035330 25.520 529 304 20 276 753 215 704 1.83e-23 106
MS.gene62735.t1 MTR_5g020000 23.656 558 327 17 279 806 206 694 6.16e-23 105
MS.gene62735.t1 MTR_4g096930 25.862 348 207 11 419 750 870 1182 3.39e-22 103
MS.gene62735.t1 MTR_1g080420 37.267 161 93 2 614 766 984 1144 6.76e-22 102
MS.gene62735.t1 MTR_1g080420 37.267 161 93 2 614 766 1115 1275 7.04e-22 102
MS.gene62735.t1 MTR_2g020000 24.366 513 294 15 279 774 213 648 2.29e-19 94.0
MS.gene62735.t1 MTR_4g118845 24.863 366 233 9 420 775 126 459 2.44e-19 94.0
MS.gene62735.t1 MTR_3g053910 22.686 551 325 16 243 772 571 1041 3.61e-19 94.0
MS.gene62735.t1 MTR_3g053910 22.686 551 325 16 243 772 600 1070 3.91e-19 93.6
MS.gene62735.t1 MTR_4g118845 25.067 371 224 10 420 774 313 645 4.94e-19 93.2
MS.gene62735.t1 MTR_5g005840 23.780 492 283 17 279 750 385 804 7.02e-19 92.4
MS.gene62735.t1 MTR_5g090090 21.480 554 328 18 243 772 617 1087 1.17e-18 92.0
MS.gene62735.t1 MTR_5g090090 21.480 554 328 18 243 772 617 1087 1.30e-18 92.0
MS.gene62735.t1 MTR_4g118720 25.000 360 208 10 420 751 1132 1457 5.06e-18 90.1
MS.gene62735.t1 MTR_1g105050 25.000 500 268 19 279 750 398 818 7.87e-18 89.4
MS.gene62735.t1 MTR_4g035100 26.131 398 240 14 421 773 1581 1969 9.30e-18 89.4
MS.gene62735.t1 MTR_4g035100 26.131 398 240 14 421 773 1581 1969 9.71e-18 89.4
MS.gene62735.t1 MTR_4g078495 36.170 141 84 2 630 770 538 672 6.98e-17 85.9
MS.gene62735.t1 MTR_4g078460 36.170 141 84 2 630 770 824 958 7.44e-17 86.3
MS.gene62735.t1 MTR_1g044120 35.897 156 93 3 608 762 981 1130 2.77e-16 84.3
MS.gene62735.t1 MTR_1g053955 31.481 162 105 2 606 767 1055 1210 8.68e-16 82.8
MS.gene62735.t1 MTR_7g045505 24.485 388 242 12 421 781 126 489 1.71e-15 81.3
MS.gene62735.t1 MTR_7g045505 24.485 388 242 12 421 781 126 489 2.48e-15 80.9
MS.gene62735.t1 MTR_7g089190 37.879 132 76 2 631 761 668 794 5.62e-15 79.7
MS.gene62735.t1 MTR_1g115215 30.769 143 93 2 631 773 887 1023 9.68e-14 75.9
MS.gene62735.t1 MTR_2g084630 34.559 136 84 2 631 762 868 1002 2.41e-12 71.2
MS.gene62735.t1 MTR_2g012830 31.081 148 94 3 627 773 1146 1286 2.48e-12 71.6
MS.gene62735.t1 MTR_4g049500 32.571 175 102 6 595 767 750 910 1.65e-11 68.6
MS.gene62735.t1 MTR_2g084630 32.836 134 80 3 631 762 874 999 3.19e-11 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62735.t1 AT1G05490 48.638 771 324 18 85 817 666 1402 0.0 655
MS.gene62735.t1 AT3G24340 46.100 718 342 13 106 816 448 1127 0.0 587
MS.gene62735.t1 AT3G42670 33.655 725 423 18 111 801 541 1241 7.83e-109 362
MS.gene62735.t1 AT3G42670 33.655 725 423 18 111 801 541 1241 7.83e-109 362
MS.gene62735.t1 AT3G42670 33.655 725 423 18 111 801 541 1241 7.83e-109 362
MS.gene62735.t1 AT3G42670 33.425 727 424 18 111 801 541 1243 2.82e-108 360
MS.gene62735.t1 AT5G20420 33.381 695 414 15 111 774 547 1223 2.18e-106 355
MS.gene62735.t1 AT2G16390 31.646 632 359 16 155 767 270 847 5.09e-88 298
MS.gene62735.t1 AT2G16390 31.646 632 359 16 155 767 273 850 5.37e-88 298
MS.gene62735.t1 AT2G21450 31.232 698 388 23 110 777 57 692 2.52e-87 292
MS.gene62735.t1 AT2G21450 31.541 688 379 23 110 767 148 773 8.79e-87 293
MS.gene62735.t1 AT2G21450 31.541 688 379 23 110 767 155 780 1.91e-86 292
MS.gene62735.t1 AT3G19210 24.694 571 332 16 239 770 176 687 2.31e-33 138
MS.gene62735.t1 AT3G19210 24.694 571 332 16 239 770 176 687 3.45e-33 138
MS.gene62735.t1 AT3G19210 24.253 569 335 17 239 770 176 685 1.32e-32 136
MS.gene62735.t1 AT2G18760 26.110 563 306 21 243 767 444 934 8.11e-29 124
MS.gene62735.t1 AT2G18760 26.110 563 306 21 243 767 385 875 8.68e-29 124
MS.gene62735.t1 AT2G18760 26.110 563 306 21 243 767 385 875 8.68e-29 124
MS.gene62735.t1 AT2G18760 26.110 563 306 21 243 767 385 875 8.68e-29 124
MS.gene62735.t1 AT2G18760 26.110 563 306 21 243 767 385 875 8.68e-29 124
MS.gene62735.t1 AT2G18760 26.110 563 306 21 243 767 385 875 8.68e-29 124
MS.gene62735.t1 AT2G18760 26.110 563 306 21 243 767 385 875 8.68e-29 124
MS.gene62735.t1 AT4G31900 22.924 602 357 19 218 794 199 718 4.33e-27 119
MS.gene62735.t1 AT4G31900 22.924 602 357 19 218 794 158 677 4.61e-27 119
MS.gene62735.t1 AT4G31900 22.924 602 357 19 218 794 199 718 6.36e-27 118
MS.gene62735.t1 AT5G63950 24.248 565 317 20 243 762 377 875 3.32e-25 113
MS.gene62735.t1 AT5G63950 25.166 453 252 15 368 762 435 858 2.29e-23 107
MS.gene62735.t1 AT5G44800 23.768 568 332 22 206 750 648 1137 3.75e-23 107
MS.gene62735.t1 AT2G25170 22.667 600 371 17 233 801 288 825 9.36e-23 105
MS.gene62735.t1 AT2G25170 22.667 600 371 17 233 801 262 799 1.03e-22 105
MS.gene62735.t1 AT2G25170 22.667 600 371 17 233 801 262 799 1.03e-22 105
MS.gene62735.t1 AT2G25170 22.667 600 371 17 233 801 279 816 1.13e-22 105
MS.gene62735.t1 AT1G08600 36.025 161 95 2 614 766 1116 1276 6.33e-21 99.4
MS.gene62735.t1 AT1G08600 36.025 161 95 2 614 766 1116 1276 6.33e-21 99.4
MS.gene62735.t1 AT1G08600 36.025 161 95 2 614 766 1116 1276 6.33e-21 99.4
MS.gene62735.t1 AT1G08600 36.025 161 95 2 614 766 1095 1255 7.40e-21 99.4
MS.gene62735.t1 AT2G13370 23.423 555 316 21 242 772 624 1093 1.74e-20 98.2
MS.gene62735.t1 AT2G13370 23.423 555 316 21 242 772 624 1093 1.74e-20 98.2
MS.gene62735.t1 AT2G13370 23.423 555 316 21 242 772 624 1093 1.74e-20 98.2
MS.gene62735.t1 AT5G66750 23.508 553 334 18 279 807 224 711 7.02e-20 95.5
MS.gene62735.t1 AT5G18620 23.197 513 300 14 279 774 216 651 3.06e-18 90.5
MS.gene62735.t1 AT5G18620 24.384 365 234 8 420 774 319 651 3.06e-18 90.5
MS.gene62735.t1 AT3G06400 23.720 371 229 8 420 774 314 646 6.85e-18 89.4
MS.gene62735.t1 AT3G06400 23.720 371 229 8 420 774 314 646 7.09e-18 89.4
MS.gene62735.t1 AT3G06400 23.720 371 229 8 420 774 314 646 7.22e-18 89.4
MS.gene62735.t1 AT5G19310 23.517 489 288 15 279 750 144 563 7.59e-18 89.0
MS.gene62735.t1 AT2G28290 24.863 366 201 10 420 751 881 1206 8.34e-18 89.7
MS.gene62735.t1 AT2G28290 24.863 366 201 10 420 751 882 1207 8.34e-18 89.7
MS.gene62735.t1 AT2G28290 24.863 366 201 10 420 751 881 1206 8.61e-18 89.7
MS.gene62735.t1 AT2G28290 24.863 366 201 10 420 751 881 1206 8.76e-18 89.7
MS.gene62735.t1 AT2G28290 24.863 366 201 10 420 751 881 1206 8.76e-18 89.7
MS.gene62735.t1 AT2G28290 24.863 366 201 10 420 751 882 1207 8.91e-18 89.4
MS.gene62735.t1 AT5G19310 23.517 489 288 15 279 750 408 827 1.10e-17 89.0
MS.gene62735.t1 AT1G03750 36.170 141 84 2 630 770 545 679 2.80e-17 87.4
MS.gene62735.t1 AT2G44980 25.840 387 217 13 421 777 181 527 4.80e-17 86.7
MS.gene62735.t1 AT5G43530 39.850 133 74 2 630 762 1128 1254 7.71e-17 86.3
MS.gene62735.t1 AT3G06010 24.432 352 211 11 420 750 528 845 1.25e-16 85.5
MS.gene62735.t1 AT2G44980 26.396 394 219 15 421 777 186 545 1.36e-16 85.1
MS.gene62735.t1 AT2G44980 26.396 394 219 15 421 777 187 546 1.45e-16 85.1
MS.gene62735.t1 AT3G12810 32.099 162 104 2 606 767 1064 1219 2.84e-16 84.3
MS.gene62735.t1 AT3G12810 32.099 162 104 2 606 767 1068 1223 3.09e-16 84.3
MS.gene62735.t1 AT5G05130 36.025 161 91 3 631 790 727 876 3.19e-16 84.0
MS.gene62735.t1 AT3G32330 23.018 391 199 12 237 605 17 327 3.19e-14 75.1
MS.gene62735.t1 AT1G11100 30.573 157 103 2 630 786 929 1079 5.58e-14 76.6
MS.gene62735.t1 AT1G11100 30.573 157 103 2 630 786 973 1123 6.39e-14 76.6
MS.gene62735.t1 AT1G11100 30.573 157 103 2 630 786 1072 1222 7.36e-14 76.6
MS.gene62735.t1 AT1G11100 30.573 157 103 2 630 786 1116 1266 7.90e-14 76.3
MS.gene62735.t1 AT1G11100 30.573 157 103 2 630 786 1115 1265 8.25e-14 76.3
MS.gene62735.t1 AT3G54280 31.973 147 93 3 629 773 1830 1971 2.70e-13 74.7
MS.gene62735.t1 AT3G54280 31.973 147 93 3 629 773 1861 2002 2.99e-13 74.7
MS.gene62735.t1 AT1G61140 31.915 141 88 3 630 769 966 1099 2.09e-12 71.6
MS.gene62735.t1 AT1G61140 31.915 141 88 3 630 769 1124 1257 2.10e-12 71.6
MS.gene62735.t1 AT3G54460 35.714 140 78 5 627 762 894 1025 2.80e-12 71.2
MS.gene62735.t1 AT3G54460 35.714 140 78 5 627 762 1191 1322 3.21e-12 71.2
MS.gene62735.t1 AT1G50410 30.137 146 90 3 631 773 828 964 4.17e-12 70.5
MS.gene62735.t1 AT1G50410 30.137 146 90 3 631 773 828 964 4.17e-12 70.5
MS.gene62735.t1 AT1G50410 30.137 146 90 3 631 773 828 964 4.17e-12 70.5
MS.gene62735.t1 AT3G20010 30.769 143 93 2 631 773 628 764 6.51e-12 69.7
MS.gene62735.t1 AT3G20010 30.769 143 93 2 631 773 603 739 6.55e-12 69.7
MS.gene62735.t1 AT3G20010 30.769 143 93 2 631 773 894 1030 1.16e-11 69.3
MS.gene62735.t1 AT3G20010 30.769 143 93 2 631 773 894 1030 1.16e-11 69.3
MS.gene62735.t1 AT3G20010 30.769 143 93 2 631 773 894 1030 1.16e-11 69.3
MS.gene62735.t1 AT5G22750 30.968 155 101 2 608 762 858 1006 1.99e-11 68.6
MS.gene62735.t1 AT1G02670 32.639 144 89 3 631 773 539 675 3.66e-11 67.4
MS.gene62735.t1 AT1G02670 32.639 144 89 3 631 773 512 648 3.91e-11 67.0
MS.gene62735.t1 AT1G02670 32.639 144 89 3 631 773 516 652 5.88e-11 66.6
MS.gene62735.t1 AT1G02670 32.639 144 89 3 631 773 527 663 6.10e-11 66.6
MS.gene62735.t1 AT1G05120 34.783 138 78 4 631 767 725 851 6.27e-11 66.6
MS.gene62735.t1 AT1G05120 34.783 138 78 4 631 767 682 808 7.33e-11 66.6

Find 171 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTACAGATTGGAAATTT+AGG 0.126449 7.3:+70245753 MS.gene62735:intron
ATATGACTTCTTTGATTTCT+TGG 0.141776 7.3:-70246960 None:intergenic
CTCCATTCCAGAACAATTTC+TGG 0.174401 7.3:+70247036 MS.gene62735:CDS
TATGGTCAGCCTTGTCTTTC+CGG 0.181588 7.3:-70246535 None:intergenic
AGCTTCTAGGGTTGTACTTC+TGG 0.196779 7.3:+70247793 MS.gene62735:CDS
GAAATCAAAGAAGTCATATT+TGG 0.244946 7.3:+70246964 MS.gene62735:CDS
ATTCCTTCTTCTTCTCAATA+TGG 0.245100 7.3:-70245262 None:intergenic
CGGAGTTGTGAACCTGTTTC+GGG 0.246918 7.3:+70247164 MS.gene62735:CDS
TTCAAAGAAACAAGCATTCT+TGG 0.249693 7.3:+70246798 MS.gene62735:CDS
AGCCTTGTCTTTCCGGTTCC+AGG 0.258151 7.3:-70246528 None:intergenic
AACAATAATCGGAAAGCATT+TGG 0.260267 7.3:-70246589 None:intergenic
ATGATTTATGTCCCTGCTTA+TGG 0.263420 7.3:-70247133 None:intergenic
TATAAAGTTTGTTTCAAATC+TGG 0.265369 7.3:-70246387 None:intergenic
GCGGAGTTGTGAACCTGTTT+CGG 0.280802 7.3:+70247163 MS.gene62735:CDS
ATTTAAAAGCAGAGGTTAAT+TGG 0.281211 7.3:-70247619 None:intergenic
AAGAAGGCTTTGAATTCATT+TGG 0.281224 7.3:+70246421 MS.gene62735:CDS
TGCTCCTTGCGCAATGCTAT+TGG 0.289113 7.3:+70245345 MS.gene62735:CDS
ATCTACAGATTGGAAATTTA+GGG 0.298187 7.3:+70245754 MS.gene62735:intron
AAAATAAGCTTCCTGGGTTA+AGG 0.301129 7.3:+70247279 MS.gene62735:CDS
TTAGCTTAGTTGGAGCTTCT+AGG 0.302332 7.3:+70247780 MS.gene62735:CDS
TTTGTTTCAAATCTGGAATT+AGG 0.316184 7.3:-70246380 None:intergenic
CGAAGTGTTATGGGATGAAT+TGG 0.316690 7.3:+70245535 MS.gene62735:CDS
AGGAGGAGAACGAAGTGTTA+TGG 0.324265 7.3:+70245525 MS.gene62735:CDS
CTTGTTGTGTTGCCCGAAAC+AGG 0.324806 7.3:-70247176 None:intergenic
TTAAGCAATCCCGAGTTATC+TGG 0.334660 7.3:+70246684 MS.gene62735:CDS
CAGAAGCGAATCATCCTTTC+CGG 0.338463 7.3:+70247011 MS.gene62735:CDS
GGAATCAGCTACAGTTTGTT+TGG 0.342280 7.3:+70246819 MS.gene62735:CDS
ATCCTTGAAAATAAGCTTCC+TGG 0.344675 7.3:+70247272 MS.gene62735:CDS
TCCTGGGTTAAGGGACTGTT+TGG 0.348954 7.3:+70247289 MS.gene62735:CDS
CACAGTTGAATCATTCTTCA+TGG 0.355596 7.3:-70245381 None:intergenic
GGAGGAGAACGAAGTGTTAT+GGG 0.356347 7.3:+70245526 MS.gene62735:CDS
CTTGCAATGAAGGCACAGTT+TGG 0.359027 7.3:+70246355 MS.gene62735:CDS
ATTGTAAAGGCGCATGGAAT+TGG 0.364774 7.3:-70247586 None:intergenic
ACTAATTTCTCTTGCAATGA+AGG 0.366544 7.3:+70246345 MS.gene62735:CDS
AAATAAGCTTCCTGGGTTAA+GGG 0.369051 7.3:+70247280 MS.gene62735:CDS
TCTGAGGGAATTAGCTTAGT+TGG 0.375316 7.3:+70247770 MS.gene62735:CDS
GGGAGGATGAATTCAAGAAA+TGG 0.377896 7.3:+70246637 MS.gene62735:CDS
AGATTTCCCTGTGAAGGTTC+AGG 0.380692 7.3:+70246243 MS.gene62735:CDS
CTGCTAGTATACTACTAACT+TGG 0.381021 7.3:+70246616 MS.gene62735:CDS
TCTGCTCGCTGAAGGGACTA+TGG 0.381344 7.3:+70247901 MS.gene62735:CDS
CAAGAAGCTGAAATTGCTAA+TGG 0.382622 7.3:+70247220 MS.gene62735:CDS
GCTCCTTGCGCAATGCTATT+GGG 0.383553 7.3:+70245346 MS.gene62735:CDS
CTTTCTTCCTGAACCTTCAC+AGG 0.384915 7.3:-70246250 None:intergenic
AGCTCAGGCGGTATCGTGTT+AGG 0.387985 7.3:-70247098 None:intergenic
ATTACGGATTGCTTGACCTT+TGG 0.391840 7.3:-70247686 None:intergenic
ATTTCTCATGCTCCTGGAAC+CGG 0.395216 7.3:+70246516 MS.gene62735:CDS
CTTGGACTCATCGTCATTAT+CGG 0.401265 7.3:-70247996 None:intergenic
ACGGATTGCTTGACCTTTGG+AGG 0.405791 7.3:-70247683 None:intergenic
AAAGCTCCCAGAAATTGTTC+TGG 0.406710 7.3:-70247043 None:intergenic
GGAAGAAAGGCATCATTTGA+TGG 0.407266 7.3:+70246264 MS.gene62735:CDS
CTAGCAGGAGCAACAATAAT+CGG 0.410720 7.3:-70246600 None:intergenic
ACAATAATCGGAAAGCATTT+GGG 0.412501 7.3:-70246588 None:intergenic
AAAATTCATATCACAGCTCT+TGG 0.412571 7.3:-70248014 None:intergenic
ATTGTTCTGGAATGGAGTTC+CGG 0.412671 7.3:-70247030 None:intergenic
TTTCCTTGAATGTTCTCTTT+CGG 0.413344 7.3:+70247427 MS.gene62735:CDS
CTTCCGAAAGAGAACATTCA+AGG 0.414255 7.3:-70247430 None:intergenic
TTCAACTGTGTTCGAGTTCA+AGG 0.414802 7.3:+70245394 MS.gene62735:CDS
ATCGTTGAAGCTATGGATTA+CGG 0.422848 7.3:-70247702 None:intergenic
ACTGAGGAAGGAGCGAGATA+TGG 0.423086 7.3:+70248105 MS.gene62735:CDS
TGCTCTCGACAAGGATCAGC+TGG 0.426412 7.3:+70247463 MS.gene62735:CDS
TGCATTATTTCTCATGCTCC+TGG 0.431774 7.3:+70246510 MS.gene62735:CDS
CCTTTGTTCATGTGCACAAA+GGG 0.432796 7.3:+70247246 MS.gene62735:CDS
AACAAAGGAAGGGATTATAA+AGG 0.438543 7.3:+70245227 MS.gene62735:CDS
TTGTACTTCTGGATGTTGTA+TGG 0.448145 7.3:+70247804 MS.gene62735:CDS
ATATGGTAAACAGGCTGAAA+AGG 0.451018 7.3:+70247934 MS.gene62735:CDS
TGGTCTTTAATTAATTGTAA+AGG 0.452462 7.3:-70247599 None:intergenic
AAGGAAGTGTTGTTCATGTC+TGG 0.458301 7.3:+70247653 MS.gene62735:CDS
TCCATTCCAGAACAATTTCT+GGG 0.462331 7.3:+70247037 MS.gene62735:CDS
AAGCTATCAGCCGAGCGTAT+AGG 0.462639 7.3:+70247846 MS.gene62735:CDS
TCGTGTTCACATCGCGATGA+TGG 0.467848 7.3:-70245810 None:intergenic
TTGCAATGAAGGCACAGTTT+GGG 0.468703 7.3:+70246356 MS.gene62735:CDS
GTAAATGTTTCACTGTTTGA+TGG 0.471326 7.3:+70246288 MS.gene62735:CDS
TAGCTTAGTTGGAGCTTCTA+GGG 0.471979 7.3:+70247781 MS.gene62735:CDS
TTCTTAAGGTTCCATATCGA+TGG 0.475228 7.3:-70245473 None:intergenic
GAAAATGTGAAGGGGAGAAA+AGG 0.475800 7.3:+70246870 MS.gene62735:CDS
GCAAAAGACCATGGAGGAGG+AGG 0.480692 7.3:+70245508 MS.gene62735:CDS
ATAGCTTGTCTTTCGACTGA+AGG 0.494216 7.3:-70247830 None:intergenic
GGAGGATGAATTCAAGAAAT+GGG 0.494983 7.3:+70246638 MS.gene62735:CDS
AGAACGCTTCACTAAGCTTA+AGG 0.496015 7.3:-70247072 None:intergenic
CTGTGAAGGTTCAGGAAGAA+AGG 0.497585 7.3:+70246251 MS.gene62735:CDS
CAAGTTAGTAGTATACTAGC+AGG 0.498458 7.3:-70246615 None:intergenic
TTCGTTCTCCTCCTCCTCCA+TGG 0.498689 7.3:-70245516 None:intergenic
GGTTTGTTAGTTCTAGATGA+AGG 0.499262 7.3:+70246930 MS.gene62735:CDS
AATTTGCATCGTTGAAGCTA+TGG 0.500809 7.3:-70247709 None:intergenic
CTCAGACCATTTAAAAGCAG+AGG 0.505543 7.3:-70247627 None:intergenic
TCCTTGAAAATAAGCTTCCT+GGG 0.507367 7.3:+70247273 MS.gene62735:CDS
TCTTAAGGTTCCATATCGAT+GGG 0.513042 7.3:-70245472 None:intergenic
GGATTGCTTAAATTGTGAAA+CGG 0.514265 7.3:-70246672 None:intergenic
GGAATCAAGTAAACAGAGAA+TGG 0.514605 7.3:+70245199 MS.gene62735:CDS
ATATGCCTTGAGAAACACTA+TGG 0.514676 7.3:-70246553 None:intergenic
TTCTTGATCTTATCATCAGA+AGG 0.516557 7.3:-70247203 None:intergenic
CCTTTGTGCACATGAACAAA+GGG 0.519201 7.3:-70247245 None:intergenic
GCCACCTTCACTGCAAGGAT+CGG 0.519684 7.3:-70246487 None:intergenic
CATCCATATTGAGAAGAAGA+AGG 0.520459 7.3:+70245259 MS.gene62735:CDS
AAGATTCTGCTAAAAGCTCC+TGG 0.520895 7.3:+70246909 MS.gene62735:CDS
CGCTGAAGGGACTATGGAGG+AGG 0.528705 7.3:+70247907 MS.gene62735:CDS
GGACTGTTTGGTGACTTTGA+AGG 0.530361 7.3:+70247301 MS.gene62735:CDS
GTTGATAGATTTCCCTGTGA+AGG 0.531771 7.3:+70246237 MS.gene62735:CDS
TAATTAATTGTAAAGGCGCA+TGG 0.533525 7.3:-70247592 None:intergenic
CCCTTTGTTCATGTGCACAA+AGG 0.533803 7.3:+70247245 MS.gene62735:CDS
GTCTCCACAGAAACCGGTGG+TGG 0.533923 7.3:-70245446 None:intergenic
CCCTTTGTGCACATGAACAA+AGG 0.536847 7.3:-70247246 None:intergenic
CAAATATATCTCACCACTAG+TGG 0.541968 7.3:+70245896 MS.gene62735:CDS
ACAGCCACCACCGGTTTCTG+TGG 0.546294 7.3:+70245442 MS.gene62735:CDS
ATCAGCCGAGCGTATAGGAT+TGG 0.547339 7.3:+70247851 MS.gene62735:CDS
GAATGGTGGAGAACAAAGGA+AGG 0.552165 7.3:+70245216 MS.gene62735:CDS
ATGCGAATAGAACCTTTGCT+TGG 0.557067 7.3:-70247733 None:intergenic
CTTAGACTGAACCCATATGA+AGG 0.561386 7.3:+70247491 MS.gene62735:CDS
AATGCCGATCCTTGCAGTGA+AGG 0.561759 7.3:+70246483 MS.gene62735:CDS
TAGTCCCTTCAGCGAGCAGA+TGG 0.563533 7.3:-70247898 None:intergenic
ATGTTCTTTACCAGATAACT+CGG 0.563939 7.3:-70246694 None:intergenic
TCCCAGAAATTGTTCTGGAA+TGG 0.564172 7.3:-70247038 None:intergenic
TTATATCGTCATCAACAAGA+AGG 0.567121 7.3:+70246405 MS.gene62735:CDS
TCTGACCAATCCTATACGCT+CGG 0.568050 7.3:-70247856 None:intergenic
ATTCAAGGAAAAGCGATGGA+TGG 0.568688 7.3:-70247415 None:intergenic
CATACCATCTGCTCGCTGAA+GGG 0.572458 7.3:+70247894 MS.gene62735:CDS
TCTACAAAAGCTTGTTCTGA+GGG 0.573558 7.3:+70247755 MS.gene62735:CDS
AGAATCTGACGCTGTATCCA+TGG 0.574662 7.3:+70245571 MS.gene62735:CDS
AAGGAAAAGCGATGGATGGA+CGG 0.575965 7.3:-70247411 None:intergenic
GAACATTCAAGGAAAAGCGA+TGG 0.581694 7.3:-70247419 None:intergenic
AATCGGAAAGCATTTGGGAA+AGG 0.582011 7.3:-70246583 None:intergenic
CGATGCAAATTGCCAAGCAA+AGG 0.584100 7.3:+70247721 MS.gene62735:CDS
ATCGATATGGAACCTTAAGA+AGG 0.586361 7.3:+70245475 MS.gene62735:CDS
AAGGAATCTATTGATAACAA+TGG 0.588038 7.3:+70245278 MS.gene62735:CDS
TCAAAGAAGTCATATTTGGA+AGG 0.589347 7.3:+70246968 MS.gene62735:CDS
ACATACCATCTGCTCGCTGA+AGG 0.589622 7.3:+70247893 MS.gene62735:CDS
TGCTAGTATACTACTAACTT+GGG 0.589951 7.3:+70246617 MS.gene62735:CDS
ATGCAGCCACCTTCACTGCA+AGG 0.591972 7.3:-70246492 None:intergenic
TGTTTGGTGACTTTGAAGGC+AGG 0.596768 7.3:+70247305 MS.gene62735:CDS
TAAGTGCGTGGTACTGAGGA+AGG 0.597578 7.3:+70248093 MS.gene62735:CDS
ATCTACAAAAGCTTGTTCTG+AGG 0.600049 7.3:+70247754 MS.gene62735:CDS
GTAAACAGGCTGAAAAGGAC+CGG 0.601881 7.3:+70247939 MS.gene62735:CDS
TCTTGATCTTATCATCAGAA+GGG 0.604374 7.3:-70247202 None:intergenic
AATGGTGGAGAACAAAGGAA+GGG 0.609814 7.3:+70245217 MS.gene62735:CDS
TTTCTTCCTGAACCTTCACA+GGG 0.609885 7.3:-70246249 None:intergenic
TGTTCTTTACCAGATAACTC+GGG 0.610730 7.3:-70246693 None:intergenic
CAGAGAATGGTGGAGAACAA+AGG 0.613980 7.3:+70245212 MS.gene62735:CDS
GATATGGAACCTTAAGAAGG+TGG 0.615242 7.3:+70245478 MS.gene62735:CDS
TCTGGAATGGAGTTCCGGAA+AGG 0.615617 7.3:-70247025 None:intergenic
ACAAGCTACACAGCCACCAC+CGG 0.619069 7.3:+70245433 MS.gene62735:CDS
GCCGATCCTTGCAGTGAAGG+TGG 0.620537 7.3:+70246486 MS.gene62735:CDS
GTGTTCGAGTTCAAGGAGGA+TGG 0.621068 7.3:+70245401 MS.gene62735:CDS
GGTGTATATTGCAAATTGTG+TGG 0.625204 7.3:+70245855 MS.gene62735:CDS
CTTAAGGTTCCATATCGATG+GGG 0.625433 7.3:-70245471 None:intergenic
AGTGCAAAAGACCATGGAGG+AGG 0.626765 7.3:+70245505 MS.gene62735:CDS
AGACATGTTTGTTAAGTGCG+TGG 0.627760 7.3:+70248081 MS.gene62735:CDS
ATCAAGTAAACAGAGAATGG+TGG 0.632548 7.3:+70245202 MS.gene62735:CDS
TGTCCCAATAGCATTGCGCA+AGG 0.633073 7.3:-70245349 None:intergenic
AAAAGCATGAAAATGTGAAG+GGG 0.633486 7.3:+70246862 MS.gene62735:CDS
GCTGACCATAGTGTTTCTCA+AGG 0.634254 7.3:+70246548 MS.gene62735:CDS
TTAAGGTTCCATATCGATGG+GGG 0.634311 7.3:-70245470 None:intergenic
AGAAGAATCTGCTCTCGACA+AGG 0.638283 7.3:+70247454 MS.gene62735:CDS
GAAACCGTGCTTACCACTAG+TGG 0.644506 7.3:-70245909 None:intergenic
ATATTGCAAATTGTGTGGTG+TGG 0.649175 7.3:+70245860 MS.gene62735:CDS
GAATTCAAGAAATGGGACAT+CGG 0.650153 7.3:+70246645 MS.gene62735:CDS
CTCCTGGAACCGGAAAGACA+AGG 0.656255 7.3:+70246526 MS.gene62735:CDS
TCATCTAGAACTAACAAACC+AGG 0.659449 7.3:-70246927 None:intergenic
GTCTGGCAAAGATCCTCCAA+AGG 0.659751 7.3:+70247670 MS.gene62735:CDS
CTTTGTTCATGTGCACAAAG+GGG 0.661128 7.3:+70247247 MS.gene62735:CDS
AACAAAGCAAGAAATGATGG+AGG 0.661151 7.3:+70245178 None:intergenic
ACCAAACAGTCCCTTAACCC+AGG 0.663907 7.3:-70247290 None:intergenic
TAGTATACTACTAACTTGGG+AGG 0.670120 7.3:+70246620 MS.gene62735:CDS
GCTCGCTGAAGGGACTATGG+AGG 0.670171 7.3:+70247904 MS.gene62735:CDS
GGTGTCTCCACAGAAACCGG+TGG 0.680014 7.3:-70245449 None:intergenic
TAGGATTGGTCAGAAGAGAG+TGG 0.680264 7.3:+70247865 MS.gene62735:CDS
AACTGTGTTCGAGTTCAAGG+AGG 0.688944 7.3:+70245397 MS.gene62735:CDS
TTGTTAAGTGCGTGGTACTG+AGG 0.691138 7.3:+70248089 MS.gene62735:CDS
GGACTCATCGTCATTATCGG+CGG 0.691472 7.3:-70247993 None:intergenic
TGGTAAAGAACATGCTGATG+CGG 0.695876 7.3:+70246704 MS.gene62735:CDS
AGCATAATATTAATGAAACG+CGG 0.743459 7.3:+70246754 MS.gene62735:CDS
AAAAGTGCAAAAGACCATGG+AGG 0.766977 7.3:+70245502 MS.gene62735:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGATTTATGTAAAATAACT+TGG + chr7.3:70245935-70245954 MS.gene62735:intron 15.0%
!!! ATTTTAATTTTGTGAACTAT+AGG + chr7.3:70245633-70245652 MS.gene62735:intron 15.0%
!! CTGTTTATATGTTTAAACTT+CGG + chr7.3:70245692-70245711 MS.gene62735:intron 20.0%
!! GAAGCTACAAAAATAAAAAA+CGG + chr7.3:70245783-70245802 MS.gene62735:CDS 20.0%
!! TGGTCTTTAATTAATTGTAA+AGG - chr7.3:70247602-70247621 None:intergenic 20.0%
!! TTATCAACATATATATCACA+AGG - chr7.3:70246175-70246194 None:intergenic 20.0%
!!! AAACTTAAATGGTCTTAAAA+TGG - chr7.3:70245604-70245623 None:intergenic 20.0%
!!! TATAAAGTTTGTTTCAAATC+TGG - chr7.3:70246390-70246409 None:intergenic 20.0%
! AGCATAATATTAATGAAACG+CGG + chr7.3:70246754-70246773 MS.gene62735:CDS 25.0%
! ATCTACAGATTGGAAATTTA+GGG + chr7.3:70245754-70245773 MS.gene62735:intron 25.0%
! ATGCATAGTTCAAACTTAAA+TGG - chr7.3:70245615-70245634 None:intergenic 25.0%
! ATTTAAAAGCAGAGGTTAAT+TGG - chr7.3:70247622-70247641 None:intergenic 25.0%
! GAAATCAAAGAAGTCATATT+TGG + chr7.3:70246964-70246983 MS.gene62735:CDS 25.0%
! GACATAAATCATCTAAAAAG+CGG + chr7.3:70247144-70247163 MS.gene62735:CDS 25.0%
! TATCTACAGATTGGAAATTT+AGG + chr7.3:70245753-70245772 MS.gene62735:intron 25.0%
! TGAATTCATTTGGAAAAACT+TGG + chr7.3:70246431-70246450 MS.gene62735:CDS 25.0%
!! AAAAAGCATGAAAATGTGAA+GGG + chr7.3:70246861-70246880 MS.gene62735:CDS 25.0%
!! AAGGAATCTATTGATAACAA+TGG + chr7.3:70245278-70245297 MS.gene62735:CDS 25.0%
!! ATATGACTTCTTTGATTTCT+TGG - chr7.3:70246963-70246982 None:intergenic 25.0%
!! GTGATATATATGTTGATAAG+AGG + chr7.3:70246176-70246195 MS.gene62735:intron 25.0%
!! TTTGTTTCAAATCTGGAATT+AGG - chr7.3:70246383-70246402 None:intergenic 25.0%
!!! AATTAACCTCTGCTTTTAAA+TGG + chr7.3:70247621-70247640 MS.gene62735:CDS 25.0%
!!! AATTTTTGTTCGAGTTTGTT+CGG + chr7.3:70247525-70247544 MS.gene62735:CDS 25.0%
!!! TTTCATGCTTTTTCTTATCT+TGG - chr7.3:70246854-70246873 None:intergenic 25.0%
AAAAAACAAGCTCAGATAAC+CGG - chr7.3:70247961-70247980 None:intergenic 30.0%
AAAATTCATATCACAGCTCT+TGG - chr7.3:70248017-70248036 None:intergenic 30.0%
AACAAAGGAAGGGATTATAA+AGG + chr7.3:70245227-70245246 MS.gene62735:CDS 30.0%
ACTAATTTCTCTTGCAATGA+AGG + chr7.3:70246345-70246364 MS.gene62735:CDS 30.0%
ATGTTCTTTACCAGATAACT+CGG - chr7.3:70246697-70246716 None:intergenic 30.0%
ATTCCTTCTTCTTCTCAATA+TGG - chr7.3:70245265-70245284 None:intergenic 30.0%
GGATTGCTTAAATTGTGAAA+CGG - chr7.3:70246675-70246694 None:intergenic 30.0%
GTAAATGTTTCACTGTTTGA+TGG + chr7.3:70246288-70246307 MS.gene62735:CDS 30.0%
TCAAAGAAGTCATATTTGGA+AGG + chr7.3:70246968-70246987 MS.gene62735:CDS 30.0%
TCTTGATCTTATCATCAGAA+GGG - chr7.3:70247205-70247224 None:intergenic 30.0%
TGCTAGTATACTACTAACTT+GGG + chr7.3:70246617-70246636 MS.gene62735:CDS 30.0%
TTATATCGTCATCAACAAGA+AGG + chr7.3:70246405-70246424 MS.gene62735:CDS 30.0%
TTCAAAGAAACAAGCATTCT+TGG + chr7.3:70246798-70246817 MS.gene62735:CDS 30.0%
TTCTTGATCTTATCATCAGA+AGG - chr7.3:70247206-70247225 None:intergenic 30.0%
TTTCCTTGAATGTTCTCTTT+CGG + chr7.3:70247427-70247446 MS.gene62735:CDS 30.0%
! AAAAAGTTTTTGCCATAAGC+AGG + chr7.3:70247121-70247140 MS.gene62735:CDS 30.0%
! AAAAGCATGAAAATGTGAAG+GGG + chr7.3:70246862-70246881 MS.gene62735:CDS 30.0%
! AAAAGTTTTTGCCATAAGCA+GGG + chr7.3:70247122-70247141 MS.gene62735:CDS 30.0%
! CTTAAATGGTCTTAAAATGG+AGG - chr7.3:70245601-70245620 None:intergenic 30.0%
! GAAAAAGCATGAAAATGTGA+AGG + chr7.3:70246860-70246879 MS.gene62735:CDS 30.0%
! TAATTAATTGTAAAGGCGCA+TGG - chr7.3:70247595-70247614 None:intergenic 30.0%
!! AACAATAATCGGAAAGCATT+TGG - chr7.3:70246592-70246611 None:intergenic 30.0%
!! AAGAAGGCTTTGAATTCATT+TGG + chr7.3:70246421-70246440 MS.gene62735:CDS 30.0%
!! ACAATAATCGGAAAGCATTT+GGG - chr7.3:70246591-70246610 None:intergenic 30.0%
!! TCAAAAGCACACATTTTATG+CGG + chr7.3:70245315-70245334 MS.gene62735:CDS 30.0%
!!! CTTGGTTACTTTTCTGATTT+TGG + chr7.3:70245953-70245972 MS.gene62735:intron 30.0%
!!! TTGTTTTGACACCTTCATAT+GGG - chr7.3:70247505-70247524 None:intergenic 30.0%
!!! TTTGTTTTGACACCTTCATA+TGG - chr7.3:70247506-70247525 None:intergenic 30.0%
AAAATAAGCTTCCTGGGTTA+AGG + chr7.3:70247279-70247298 MS.gene62735:CDS 35.0%
AAATAAGCTTCCTGGGTTAA+GGG + chr7.3:70247280-70247299 MS.gene62735:CDS 35.0%
AATTTGCATCGTTGAAGCTA+TGG - chr7.3:70247712-70247731 None:intergenic 35.0%
ACTATGGAGGAGGAAAAATA+TGG + chr7.3:70247917-70247936 MS.gene62735:CDS 35.0%
ATATGCCTTGAGAAACACTA+TGG - chr7.3:70246556-70246575 None:intergenic 35.0%
ATATGGTAAACAGGCTGAAA+AGG + chr7.3:70247934-70247953 MS.gene62735:CDS 35.0%
ATATTGCAAATTGTGTGGTG+TGG + chr7.3:70245860-70245879 MS.gene62735:CDS 35.0%
ATCAAGTAAACAGAGAATGG+TGG + chr7.3:70245202-70245221 MS.gene62735:CDS 35.0%
ATCCTTGAAAATAAGCTTCC+TGG + chr7.3:70247272-70247291 MS.gene62735:CDS 35.0%
ATCGATATGGAACCTTAAGA+AGG + chr7.3:70245475-70245494 MS.gene62735:CDS 35.0%
ATGATTTATGTCCCTGCTTA+TGG - chr7.3:70247136-70247155 None:intergenic 35.0%
CAAATATATCTCACCACTAG+TGG + chr7.3:70245896-70245915 MS.gene62735:CDS 35.0%
CAAGAAGCTGAAATTGCTAA+TGG + chr7.3:70247220-70247239 MS.gene62735:CDS 35.0%
CAAGTTAGTAGTATACTAGC+AGG - chr7.3:70246618-70246637 None:intergenic 35.0%
CACAGTTGAATCATTCTTCA+TGG - chr7.3:70245384-70245403 None:intergenic 35.0%
CATCCATATTGAGAAGAAGA+AGG + chr7.3:70245259-70245278 MS.gene62735:CDS 35.0%
CTGCTAGTATACTACTAACT+TGG + chr7.3:70246616-70246635 MS.gene62735:CDS 35.0%
CTTAAAATGGAGGATTACCA+TGG - chr7.3:70245591-70245610 None:intergenic 35.0%
GAATTCAAGAAATGGGACAT+CGG + chr7.3:70246645-70246664 MS.gene62735:CDS 35.0%
GGAATCAAGTAAACAGAGAA+TGG + chr7.3:70245199-70245218 MS.gene62735:CDS 35.0%
GGAGGAAAAATATGGTAAAC+AGG + chr7.3:70247925-70247944 MS.gene62735:CDS 35.0%
GGAGGATGAATTCAAGAAAT+GGG + chr7.3:70246638-70246657 MS.gene62735:CDS 35.0%
GGATGACAAAAATGATTTCG+TGG + chr7.3:70246775-70246794 MS.gene62735:CDS 35.0%
GGTGTATATTGCAAATTGTG+TGG + chr7.3:70245855-70245874 MS.gene62735:CDS 35.0%
TAGTATACTACTAACTTGGG+AGG + chr7.3:70246620-70246639 MS.gene62735:CDS 35.0%
TCCATTCCAGAACAATTTCT+GGG + chr7.3:70247037-70247056 MS.gene62735:CDS 35.0%
TCCTTGAAAATAAGCTTCCT+GGG + chr7.3:70247273-70247292 MS.gene62735:CDS 35.0%
TCTTAAGGTTCCATATCGAT+GGG - chr7.3:70245475-70245494 None:intergenic 35.0%
TGTTCTTTACCAGATAACTC+GGG - chr7.3:70246696-70246715 None:intergenic 35.0%
TTCATTTGGAAAAACTTGGC+AGG + chr7.3:70246435-70246454 MS.gene62735:CDS 35.0%
TTCTTAAGGTTCCATATCGA+TGG - chr7.3:70245476-70245495 None:intergenic 35.0%
TTGTACTTCTGGATGTTGTA+TGG + chr7.3:70247804-70247823 MS.gene62735:CDS 35.0%
! ATCGTTGAAGCTATGGATTA+CGG - chr7.3:70247705-70247724 None:intergenic 35.0%
! ATCTACAAAAGCTTGTTCTG+AGG + chr7.3:70247754-70247773 MS.gene62735:CDS 35.0%
! GTGTGTTACTATCTACAGAT+TGG + chr7.3:70245744-70245763 MS.gene62735:intron 35.0%
! TCATCTAGAACTAACAAACC+AGG - chr7.3:70246930-70246949 None:intergenic 35.0%
! TCTACAAAAGCTTGTTCTGA+GGG + chr7.3:70247755-70247774 MS.gene62735:CDS 35.0%
! TTTTAAATGGTCTGAGGGAA+AGG + chr7.3:70247634-70247653 MS.gene62735:CDS 35.0%
!! GGTTTGTTAGTTCTAGATGA+AGG + chr7.3:70246930-70246949 MS.gene62735:CDS 35.0%
!! TCTGCTTTTAAATGGTCTGA+GGG + chr7.3:70247629-70247648 MS.gene62735:CDS 35.0%
!! TGCAAAGTATTGTGTACTTG+CGG + chr7.3:70246032-70246051 MS.gene62735:intron 35.0%
!!! CAAAAACTTTTTAGCTCAGG+CGG - chr7.3:70247113-70247132 None:intergenic 35.0%
!!! TGGCAAAAACTTTTTAGCTC+AGG - chr7.3:70247116-70247135 None:intergenic 35.0%
AAAGCTCCCAGAAATTGTTC+TGG - chr7.3:70247046-70247065 None:intergenic 40.0%
AAGATTCTGCTAAAAGCTCC+TGG + chr7.3:70246909-70246928 MS.gene62735:CDS 40.0%
AGAACGCTTCACTAAGCTTA+AGG - chr7.3:70247075-70247094 None:intergenic 40.0%
ATGCGAATAGAACCTTTGCT+TGG - chr7.3:70247736-70247755 None:intergenic 40.0%
ATTCAAGGAAAAGCGATGGA+TGG - chr7.3:70247418-70247437 None:intergenic 40.0%
CCTTTGTGCACATGAACAAA+GGG - chr7.3:70247248-70247267 None:intergenic 40.0%
CCTTTGTTCATGTGCACAAA+GGG + chr7.3:70247246-70247265 MS.gene62735:CDS 40.0%
CTAGCAGGAGCAACAATAAT+CGG - chr7.3:70246603-70246622 None:intergenic 40.0%
CTCAGACCATTTAAAAGCAG+AGG - chr7.3:70247630-70247649 None:intergenic 40.0%
CTCCATTCCAGAACAATTTC+TGG + chr7.3:70247036-70247055 MS.gene62735:CDS 40.0%
CTTAAGGTTCCATATCGATG+GGG - chr7.3:70245474-70245493 None:intergenic 40.0%
CTTAGACTGAACCCATATGA+AGG + chr7.3:70247491-70247510 MS.gene62735:CDS 40.0%
CTTCCGAAAGAGAACATTCA+AGG - chr7.3:70247433-70247452 None:intergenic 40.0%
CTTGGACTCATCGTCATTAT+CGG - chr7.3:70247999-70248018 None:intergenic 40.0%
CTTTGTTCATGTGCACAAAG+GGG + chr7.3:70247247-70247266 MS.gene62735:CDS 40.0%
GAAAATGTGAAGGGGAGAAA+AGG + chr7.3:70246870-70246889 MS.gene62735:CDS 40.0%
GAACATTCAAGGAAAAGCGA+TGG - chr7.3:70247422-70247441 None:intergenic 40.0%
GATATGGAACCTTAAGAAGG+TGG + chr7.3:70245478-70245497 MS.gene62735:CDS 40.0%
GGGAGGATGAATTCAAGAAA+TGG + chr7.3:70246637-70246656 MS.gene62735:CDS 40.0%
TAGCTTAGTTGGAGCTTCTA+GGG + chr7.3:70247781-70247800 MS.gene62735:CDS 40.0%
TGGTAAAGAACATGCTGATG+CGG + chr7.3:70246704-70246723 MS.gene62735:CDS 40.0%
TTAAGCAATCCCGAGTTATC+TGG + chr7.3:70246684-70246703 MS.gene62735:CDS 40.0%
TTAAGGTTCCATATCGATGG+GGG - chr7.3:70245473-70245492 None:intergenic 40.0%
TTAGCTTAGTTGGAGCTTCT+AGG + chr7.3:70247780-70247799 MS.gene62735:CDS 40.0%
TTTCTTCCTGAACCTTCACA+GGG - chr7.3:70246252-70246271 None:intergenic 40.0%
! AAAAGTGCAAAAGACCATGG+AGG + chr7.3:70245502-70245521 MS.gene62735:CDS 40.0%
! AATGGTGGAGAACAAAGGAA+GGG + chr7.3:70245217-70245236 MS.gene62735:CDS 40.0%
! ACTTTTCTCGACGAAGAGAT+TGG + chr7.3:70245834-70245853 MS.gene62735:CDS 40.0%
! AGACATGTTTGTTAAGTGCG+TGG + chr7.3:70248081-70248100 MS.gene62735:CDS 40.0%
! ATAGCTTGTCTTTCGACTGA+AGG - chr7.3:70247833-70247852 None:intergenic 40.0%
! ATTACGGATTGCTTGACCTT+TGG - chr7.3:70247689-70247708 None:intergenic 40.0%
! ATTGTAAAGGCGCATGGAAT+TGG - chr7.3:70247589-70247608 None:intergenic 40.0%
! CGAAGTGTTATGGGATGAAT+TGG + chr7.3:70245535-70245554 MS.gene62735:CDS 40.0%
! GGAAAAAGTGCAAAAGACCA+TGG + chr7.3:70245499-70245518 MS.gene62735:CDS 40.0%
! GGAATCAGCTACAGTTTGTT+TGG + chr7.3:70246819-70246838 MS.gene62735:CDS 40.0%
! GTTGATAGATTTCCCTGTGA+AGG + chr7.3:70246237-70246256 MS.gene62735:CDS 40.0%
! TCTGAGGGAATTAGCTTAGT+TGG + chr7.3:70247770-70247789 MS.gene62735:CDS 40.0%
! TGCATTATTTCTCATGCTCC+TGG + chr7.3:70246510-70246529 MS.gene62735:CDS 40.0%
! TTCAACTGTGTTCGAGTTCA+AGG + chr7.3:70245394-70245413 MS.gene62735:CDS 40.0%
! TTGCAATGAAGGCACAGTTT+GGG + chr7.3:70246356-70246375 MS.gene62735:CDS 40.0%
!! AAGGAAGTGTTGTTCATGTC+TGG + chr7.3:70247653-70247672 MS.gene62735:CDS 40.0%
!! AATCGGAAAGCATTTGGGAA+AGG - chr7.3:70246586-70246605 None:intergenic 40.0%
!! ACCCAGGAAGCTTATTTTCA+AGG - chr7.3:70247277-70247296 None:intergenic 40.0%
!! ATTGTTCTGGAATGGAGTTC+CGG - chr7.3:70247033-70247052 None:intergenic 40.0%
!! CTCTGCTTTTAAATGGTCTG+AGG + chr7.3:70247628-70247647 MS.gene62735:CDS 40.0%
!! GGAAGAAAGGCATCATTTGA+TGG + chr7.3:70246264-70246283 MS.gene62735:CDS 40.0%
!! TCCCAGAAATTGTTCTGGAA+TGG - chr7.3:70247041-70247060 None:intergenic 40.0%
!!! ACTTGTTTTACACGTGACAC+AGG + chr7.3:70246215-70246234 MS.gene62735:intron 40.0%
!!! GCACTTTTTCCACCTTCTTA+AGG - chr7.3:70245490-70245509 None:intergenic 40.0%
AAGGAAAAGCGATGGATGGA+CGG - chr7.3:70247414-70247433 None:intergenic 45.0%
AGAAGAATCTGCTCTCGACA+AGG + chr7.3:70247454-70247473 MS.gene62735:CDS 45.0%
AGAATCTGACGCTGTATCCA+TGG + chr7.3:70245571-70245590 MS.gene62735:CDS 45.0%
AGATTTCCCTGTGAAGGTTC+AGG + chr7.3:70246243-70246262 MS.gene62735:CDS 45.0%
ATTTCTCATGCTCCTGGAAC+CGG + chr7.3:70246516-70246535 MS.gene62735:CDS 45.0%
CAGAAGCGAATCATCCTTTC+CGG + chr7.3:70247011-70247030 MS.gene62735:CDS 45.0%
CCCTTTGTGCACATGAACAA+AGG - chr7.3:70247249-70247268 None:intergenic 45.0%
CCCTTTGTTCATGTGCACAA+AGG + chr7.3:70247245-70247264 MS.gene62735:CDS 45.0%
CGATGCAAATTGCCAAGCAA+AGG + chr7.3:70247721-70247740 MS.gene62735:CDS 45.0%
CTGTGAAGGTTCAGGAAGAA+AGG + chr7.3:70246251-70246270 MS.gene62735:CDS 45.0%
CTTTCTTCCTGAACCTTCAC+AGG - chr7.3:70246253-70246272 None:intergenic 45.0%
GCGGCTAAAGCAGTACTATT+TGG + chr7.3:70246051-70246070 MS.gene62735:intron 45.0%
GCTGACCATAGTGTTTCTCA+AGG + chr7.3:70246548-70246567 MS.gene62735:CDS 45.0%
GTAAACAGGCTGAAAAGGAC+CGG + chr7.3:70247939-70247958 MS.gene62735:CDS 45.0%
TCTGACCAATCCTATACGCT+CGG - chr7.3:70247859-70247878 None:intergenic 45.0%
TTGTTAAGTGCGTGGTACTG+AGG + chr7.3:70248089-70248108 MS.gene62735:CDS 45.0%
! AACTGTGTTCGAGTTCAAGG+AGG + chr7.3:70245397-70245416 MS.gene62735:CDS 45.0%
! AGGAGGAGAACGAAGTGTTA+TGG + chr7.3:70245525-70245544 MS.gene62735:CDS 45.0%
! CAGAGAATGGTGGAGAACAA+AGG + chr7.3:70245212-70245231 MS.gene62735:CDS 45.0%
! CTTGCAATGAAGGCACAGTT+TGG + chr7.3:70246355-70246374 MS.gene62735:CDS 45.0%
! GAATGGTGGAGAACAAAGGA+AGG + chr7.3:70245216-70245235 MS.gene62735:CDS 45.0%
! GGACTGTTTGGTGACTTTGA+AGG + chr7.3:70247301-70247320 MS.gene62735:CDS 45.0%
! GGAGGAGAACGAAGTGTTAT+GGG + chr7.3:70245526-70245545 MS.gene62735:CDS 45.0%
! TATGGTCAGCCTTGTCTTTC+CGG - chr7.3:70246538-70246557 None:intergenic 45.0%
! TGTTTGGTGACTTTGAAGGC+AGG + chr7.3:70247305-70247324 MS.gene62735:CDS 45.0%
!! AGCTTCTAGGGTTGTACTTC+TGG + chr7.3:70247793-70247812 MS.gene62735:CDS 45.0%
!! TAGGATTGGTCAGAAGAGAG+TGG + chr7.3:70247865-70247884 MS.gene62735:CDS 45.0%
AAGCTATCAGCCGAGCGTAT+AGG + chr7.3:70247846-70247865 MS.gene62735:CDS 50.0%
AATGCCGATCCTTGCAGTGA+AGG + chr7.3:70246483-70246502 MS.gene62735:CDS 50.0%
ACATACCATCTGCTCGCTGA+AGG + chr7.3:70247893-70247912 MS.gene62735:CDS 50.0%
ACCAAACAGTCCCTTAACCC+AGG - chr7.3:70247293-70247312 None:intergenic 50.0%
ACTGAGGAAGGAGCGAGATA+TGG + chr7.3:70248105-70248124 MS.gene62735:CDS 50.0%
AGTGCAAAAGACCATGGAGG+AGG + chr7.3:70245505-70245524 MS.gene62735:CDS 50.0%
ATCAGCCGAGCGTATAGGAT+TGG + chr7.3:70247851-70247870 MS.gene62735:CDS 50.0%
CATACCATCTGCTCGCTGAA+GGG + chr7.3:70247894-70247913 MS.gene62735:CDS 50.0%
CGGAGTTGTGAACCTGTTTC+GGG + chr7.3:70247164-70247183 MS.gene62735:CDS 50.0%
CTCACCACTAGTGGTAAGCA+CGG + chr7.3:70245905-70245924 MS.gene62735:intron 50.0%
GAAACCGTGCTTACCACTAG+TGG - chr7.3:70245912-70245931 None:intergenic 50.0%
GCGGAGTTGTGAACCTGTTT+CGG + chr7.3:70247163-70247182 MS.gene62735:CDS 50.0%
GCTCCTTGCGCAATGCTATT+GGG + chr7.3:70245346-70245365 MS.gene62735:CDS 50.0%
GGACTCATCGTCATTATCGG+CGG - chr7.3:70247996-70248015 None:intergenic 50.0%
GTCTGGCAAAGATCCTCCAA+AGG + chr7.3:70247670-70247689 MS.gene62735:CDS 50.0%
TCCTGGGTTAAGGGACTGTT+TGG + chr7.3:70247289-70247308 MS.gene62735:CDS 50.0%
TCGTGTTCACATCGCGATGA+TGG - chr7.3:70245813-70245832 None:intergenic 50.0%
TGCTCCTTGCGCAATGCTAT+TGG + chr7.3:70245345-70245364 MS.gene62735:CDS 50.0%
TGTCCCAATAGCATTGCGCA+AGG - chr7.3:70245352-70245371 None:intergenic 50.0%
! ACGGATTGCTTGACCTTTGG+AGG - chr7.3:70247686-70247705 None:intergenic 50.0%
! CTTGTTGTGTTGCCCGAAAC+AGG - chr7.3:70247179-70247198 None:intergenic 50.0%
! GTGTTCGAGTTCAAGGAGGA+TGG + chr7.3:70245401-70245420 MS.gene62735:CDS 50.0%
! TAAGTGCGTGGTACTGAGGA+AGG + chr7.3:70248093-70248112 MS.gene62735:CDS 50.0%
! TCTGGAATGGAGTTCCGGAA+AGG - chr7.3:70247028-70247047 None:intergenic 50.0%
ACAAGCTACACAGCCACCAC+CGG + chr7.3:70245433-70245452 MS.gene62735:CDS 55.0%
AGCTCAGGCGGTATCGTGTT+AGG - chr7.3:70247101-70247120 None:intergenic 55.0%
ATGCAGCCACCTTCACTGCA+AGG - chr7.3:70246495-70246514 None:intergenic 55.0%
CTCCTGGAACCGGAAAGACA+AGG + chr7.3:70246526-70246545 MS.gene62735:CDS 55.0%
GCAAAAGACCATGGAGGAGG+AGG + chr7.3:70245508-70245527 MS.gene62735:CDS 55.0%
GCCACCTTCACTGCAAGGAT+CGG - chr7.3:70246490-70246509 None:intergenic 55.0%
TAGTCCCTTCAGCGAGCAGA+TGG - chr7.3:70247901-70247920 None:intergenic 55.0%
TCTGCTCGCTGAAGGGACTA+TGG + chr7.3:70247901-70247920 MS.gene62735:CDS 55.0%
TGCTCTCGACAAGGATCAGC+TGG + chr7.3:70247463-70247482 MS.gene62735:CDS 55.0%
TGGAGACACCCCCATCGATA+TGG + chr7.3:70245462-70245481 MS.gene62735:CDS 55.0%
TTCGTTCTCCTCCTCCTCCA+TGG - chr7.3:70245519-70245538 None:intergenic 55.0%
! AGCCTTGTCTTTCCGGTTCC+AGG - chr7.3:70246531-70246550 None:intergenic 55.0%
CGCTGAAGGGACTATGGAGG+AGG + chr7.3:70247907-70247926 MS.gene62735:CDS 60.0%
GCCGATCCTTGCAGTGAAGG+TGG + chr7.3:70246486-70246505 MS.gene62735:CDS 60.0%
GCTCGCTGAAGGGACTATGG+AGG + chr7.3:70247904-70247923 MS.gene62735:CDS 60.0%
GGTGTCTCCACAGAAACCGG+TGG - chr7.3:70245452-70245471 None:intergenic 60.0%
GTCTCCACAGAAACCGGTGG+TGG - chr7.3:70245449-70245468 None:intergenic 60.0%
!! ACAGCCACCACCGGTTTCTG+TGG + chr7.3:70245442-70245461 MS.gene62735:CDS 60.0%
!! GGGGGTGTCTCCACAGAAAC+CGG - chr7.3:70245455-70245474 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 70245191 70248132 70245191 ID=MS.gene62735
chr7.3 mRNA 70245191 70248132 70245191 ID=MS.gene62735.t1;Parent=MS.gene62735
chr7.3 exon 70245191 70245592 70245191 ID=MS.gene62735.t1.exon1;Parent=MS.gene62735.t1
chr7.3 CDS 70245191 70245592 70245191 ID=cds.MS.gene62735.t1;Parent=MS.gene62735.t1
chr7.3 exon 70245762 70245917 70245762 ID=MS.gene62735.t1.exon2;Parent=MS.gene62735.t1
chr7.3 CDS 70245762 70245917 70245762 ID=cds.MS.gene62735.t1;Parent=MS.gene62735.t1
chr7.3 exon 70246237 70248132 70246237 ID=MS.gene62735.t1.exon3;Parent=MS.gene62735.t1
chr7.3 CDS 70246237 70248132 70246237 ID=cds.MS.gene62735.t1;Parent=MS.gene62735.t1
Gene Sequence

>MS.gene62735

ATGATGGAGGAATCAAGTAAACAGAGAATGGTGGAGAACAAAGGAAGGGATTATAAAGGTATAGCTAACATCCATATTGAGAAGAAGAAGGAATCTATTGATAACAATGGTTTGATACAAAGCGTCAAAAGCACACATTTTATGCGGAATGAGTTGCTCCTTGCGCAATGCTATTGGGACAGAAAGAATACCATGAAGAATGATTCAACTGTGTTCGAGTTCAAGGAGGATGGCGTTGATCGACAAGCTACACAGCCACCACCGGTTTCTGTGGAGACACCCCCATCGATATGGAACCTTAAGAAGGTGGAAAAAGTGCAAAAGACCATGGAGGAGGAGGAGAACGAAGTGTTATGGGATGAATTGGATACAGTTCTTAGAGAATCTGACGCTGTATCCATGGTAATCCTCCATTTTAAGACCATTTAAGTTTGAACTATGCATTTTAATTTTGTGAACTATAGGACTAGTGCTCAAGCTGATTTTAATTTTTATTTTAAACTGTTTATATGTTTAAACTTCGGTCTAAGCAATATCTATATGTTTATACATAGTGTGTTACTATCTACAGATTGGAAATTTAGGGACAAATGAAGCTACAAAAATAAAAAACGGAAGTCCATCATCGCGATGTGAACACGACACTTTTCTCGACGAAGAGATTGGTGTATATTGCAAATTGTGTGGTGTGGTTATTACTGAGATCAAATATATCTCACCACTAGTGGTAAGCACGGTTTCATATTGATTTATGTAAAATAACTTGGTTACTTTTCTGATTTTGGTCTTGAATAGTGAAATTTGAACACATCACTTTTGATTGCATTATGCAGCGATTAAATGCAAAGTATTGTGTACTTGCGGCTAAAGCAGTACTATTTGGAACATAACACTAATAAATTTGTTGCTGTTAGATTCAATAAGTTTCTGCTGTTAGATTCAAATTAAATTTCATATAGTTGTATGTTTGTTCTGATTTTACCTTGTGATATATATGTTGATAAGAGGCACTGATATTTTGCATACTTGTTTTACACGTGACACAGGTTGATAGATTTCCCTGTGAAGGTTCAGGAAGAAAGGCATCATTTGATGGAGTAAATGTTTCACTGTTTGATGGATCTCAATTCAATGTTTATGATAAAGATTCAGAAACTAATTTCTCTTGCAATGAAGGCACAGTTTGGGACCTAATTCCAGATTTGAAACAAACTTTATATCGTCATCAACAAGAAGGCTTTGAATTCATTTGGAAAAACTTGGCAGGAAGTGTCAAGCTTCAAAAGTTAAAGAATGCCGATCCTTGCAGTGAAGGTGGCTGCATTATTTCTCATGCTCCTGGAACCGGAAAGACAAGGCTGACCATAGTGTTTCTCAAGGCATATTTGAAAGCCTTTCCCAAATGCTTTCCGATTATTGTTGCTCCTGCTAGTATACTACTAACTTGGGAGGATGAATTCAAGAAATGGGACATCGGAGTTCCGTTTCACAATTTAAGCAATCCCGAGTTATCTGGTAAAGAACATGCTGATGCGGTTGAAACGTTTGATCAGTATAATACACAGCATAATATTAATGAAACGCGGATGACAAAAATGATTTCGTGGTTCAAAGAAACAAGCATTCTTGGAATCAGCTACAGTTTGTTTGGAAAAAAATGCCAAGATAAGAAAAAGCATGAAAATGTGAAGGGGAGAAAAGGAAACTGTGATATGCGAAAGATTCTGCTAAAAGCTCCTGGTTTGTTAGTTCTAGATGAAGGACACACACCAAGAAATCAAAGAAGTCATATTTGGAAGGTGTTGTCGAAGATTCAAGCGCAGAAGCGAATCATCCTTTCCGGAACTCCATTCCAGAACAATTTCTGGGAGCTTTACAGCACCTTAAGCTTAGTGAAGCGTTCTTTTCCTAACACGATACCGCCTGAGCTAAAAAGTTTTTGCCATAAGCAGGGACATAAATCATCTAAAAAGCGGAGTTGTGAACCTGTTTCGGGCAACACAACAAGAGACCCTTCTGATGATAAGATCAAGAAGCTGAAATTGCTAATGGATCCCTTTGTTCATGTGCACAAAGGGGCGATCCTTGAAAATAAGCTTCCTGGGTTAAGGGACTGTTTGGTGACTTTGAAGGCAGGTAGTTTGCAGAATGAAATTCTGAAGAGCATTAAACGTTCTAAGAACACAATATTTAACTTTGAACGTAAGATAGCATTGACATCCGTCCATCCATCGCTTTTCCTTGAATGTTCTCTTTCGGAAGAAGAAGAATCTGCTCTCGACAAGGATCAGCTGGAAAAGCTTAGACTGAACCCATATGAAGGTGTCAAAACAAAATTTTTGTTCGAGTTTGTTCGGCTCTGCGATGCTTTTCACGAGAAAGTTCTTGTGTTCAGCCAATTCCATGCGCCTTTACAATTAATTAAAGACCAATTAACCTCTGCTTTTAAATGGTCTGAGGGAAAGGAAGTGTTGTTCATGTCTGGCAAAGATCCTCCAAAGGTCAAGCAATCCGTAATCCATAGCTTCAACGATGCAAATTGCCAAGCAAAGGTTCTATTCGCATCTACAAAAGCTTGTTCTGAGGGAATTAGCTTAGTTGGAGCTTCTAGGGTTGTACTTCTGGATGTTGTATGGAATCCTTCAGTCGAAAGACAAGCTATCAGCCGAGCGTATAGGATTGGTCAGAAGAGAGTGGTATACACATACCATCTGCTCGCTGAAGGGACTATGGAGGAGGAAAAATATGGTAAACAGGCTGAAAAGGACCGGTTATCTGAGCTTGTTTTTTCAGAGAAAAATGCCGCCGATAATGACGATGAGTCCAAGAGCTGTGATATGAATTTTGAAGATAGAGTTCTTGATCATATGACTCAGCATGAAAATCTCGAAGACATGTTTGTTAAGTGCGTGGTACTGAGGAAGGAGCGAGATATGGTCTGA
Protein sequence

>MS.gene62735.t1

MMEESSKQRMVENKGRDYKGIANIHIEKKKESIDNNGLIQSVKSTHFMRNELLLAQCYWDRKNTMKNDSTVFEFKEDGVDRQATQPPPVSVETPPSIWNLKKVEKVQKTMEEEENEVLWDELDTVLRESDAVSMIGNLGTNEATKIKNGSPSSRCEHDTFLDEEIGVYCKLCGVVITEIKYISPLVVDRFPCEGSGRKASFDGVNVSLFDGSQFNVYDKDSETNFSCNEGTVWDLIPDLKQTLYRHQQEGFEFIWKNLAGSVKLQKLKNADPCSEGGCIISHAPGTGKTRLTIVFLKAYLKAFPKCFPIIVAPASILLTWEDEFKKWDIGVPFHNLSNPELSGKEHADAVETFDQYNTQHNINETRMTKMISWFKETSILGISYSLFGKKCQDKKKHENVKGRKGNCDMRKILLKAPGLLVLDEGHTPRNQRSHIWKVLSKIQAQKRIILSGTPFQNNFWELYSTLSLVKRSFPNTIPPELKSFCHKQGHKSSKKRSCEPVSGNTTRDPSDDKIKKLKLLMDPFVHVHKGAILENKLPGLRDCLVTLKAGSLQNEILKSIKRSKNTIFNFERKIALTSVHPSLFLECSLSEEEESALDKDQLEKLRLNPYEGVKTKFLFEFVRLCDAFHEKVLVFSQFHAPLQLIKDQLTSAFKWSEGKEVLFMSGKDPPKVKQSVIHSFNDANCQAKVLFASTKACSEGISLVGASRVVLLDVVWNPSVERQAISRAYRIGQKRVVYTYHLLAEGTMEEEKYGKQAEKDRLSELVFSEKNAADNDDESKSCDMNFEDRVLDHMTQHENLEDMFVKCVVLRKERDMV