Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62925.t1 | XP_003605958.1 | 95.7 | 94 | 4 | 0 | 1 | 94 | 1 | 94 | 1.10E-36 | 162.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62925.t1 | G7JEQ0 | 95.7 | 94 | 4 | 0 | 1 | 94 | 1 | 94 | 8.1e-37 | 162.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053508 | MS.gene62925 | 0.825755 | 3.94E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62925.t1 | MTR_4g049540 | 94.737 | 95 | 5 | 0 | 1 | 95 | 1 | 95 | 2.75e-59 | 181 |
| MS.gene62925.t1 | MTR_2g023880 | 64.211 | 95 | 33 | 1 | 1 | 94 | 1 | 95 | 3.75e-38 | 128 |
| MS.gene62925.t1 | MTR_5g093360 | 47.368 | 95 | 50 | 0 | 1 | 95 | 1 | 95 | 1.66e-29 | 102 |
| MS.gene62925.t1 | MTR_2g088060 | 54.167 | 96 | 43 | 1 | 1 | 95 | 1 | 96 | 7.63e-27 | 99.0 |
| MS.gene62925.t1 | MTR_7g056133 | 40.625 | 96 | 56 | 1 | 1 | 95 | 1 | 96 | 3.03e-18 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62925.t1 | AT5G42570 | 63.158 | 95 | 35 | 0 | 1 | 95 | 1 | 95 | 1.33e-39 | 131 |
| MS.gene62925.t1 | AT1G11905 | 54.639 | 97 | 44 | 0 | 1 | 97 | 1 | 97 | 5.55e-35 | 120 |
| MS.gene62925.t1 | AT1G11905 | 55.319 | 94 | 42 | 0 | 1 | 94 | 1 | 94 | 2.60e-34 | 118 |
| MS.gene62925.t1 | AT3G20450 | 47.423 | 97 | 50 | 1 | 1 | 96 | 5 | 101 | 2.12e-22 | 85.5 |
| MS.gene62925.t1 | AT5G48660 | 43.750 | 96 | 53 | 1 | 1 | 95 | 1 | 96 | 1.05e-19 | 80.5 |
| MS.gene62925.t1 | AT3G07190 | 40.625 | 96 | 56 | 1 | 1 | 95 | 1 | 96 | 2.29e-17 | 74.3 |
Find 21 sgRNAs with CRISPR-Local
Find 115 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAACCCCAGTTCGAAAAT+TGG | 0.173980 | 4.2:-52095494 | MS.gene62925:CDS |
| TATGCTTGAAGCTTCTTTAA+TGG | 0.189332 | 4.2:-52095296 | MS.gene62925:CDS |
| TCAACCAAAGAGTTATTAAA+AGG | 0.248921 | 4.2:+52093579 | None:intergenic |
| AAAATTGGTGATTGTTTCAT+TGG | 0.277616 | 4.2:-52095479 | MS.gene62925:CDS |
| TTGTTCTAGTCTTTATAGTA+TGG | 0.304011 | 4.2:-52095389 | MS.gene62925:CDS |
| ATGCTTGAAGCTTCTTTAAT+GGG | 0.346757 | 4.2:-52095295 | MS.gene62925:intron |
| TTTAATAACTCTTTGGTTGA+AGG | 0.386200 | 4.2:-52093576 | MS.gene62925:CDS |
| AGAGTTATTAAAAGGTTTGA+AGG | 0.420990 | 4.2:+52093587 | None:intergenic |
| CAGCTTCAACCGTACGTTGT+TGG | 0.429307 | 4.2:+52095359 | None:intergenic |
| TTAATAACTCTTTGGTTGAA+GGG | 0.462937 | 4.2:-52093575 | MS.gene62925:CDS |
| GACATAAGAACTTGATCAGT+AGG | 0.466093 | 4.2:+52095325 | None:intergenic |
| GGGGTGTTTGATTCAAAAGT+TGG | 0.516085 | 4.2:-52093555 | MS.gene62925:CDS |
| TTGATTCAAAAGTTGGCTGA+AGG | 0.541738 | 4.2:-52093548 | None:intergenic |
| CCAATCGTTGTTACTACAGT+TGG | 0.544982 | 4.2:-52095433 | MS.gene62925:CDS |
| TAATAACTCTTTGGTTGAAG+GGG | 0.552787 | 4.2:-52093574 | MS.gene62925:CDS |
| GATCGAGTCAAAAGAGGTCG+TGG | 0.559355 | 4.2:-52095457 | MS.gene62925:CDS |
| CAACAACGTACGGTTGAAGC+TGG | 0.561185 | 4.2:-52095358 | MS.gene62925:CDS |
| TCATTGGATCGAGTCAAAAG+AGG | 0.561683 | 4.2:-52095463 | MS.gene62925:CDS |
| GGCTAAGATCCAACAACGTA+CGG | 0.569797 | 4.2:-52095368 | MS.gene62925:CDS |
| ACATAAGAACTTGATCAGTA+GGG | 0.596833 | 4.2:+52095326 | None:intergenic |
| CCAACTGTAGTAACAACGAT+TGG | 0.627596 | 4.2:+52095433 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTACTTAAAACTAAAAT+AGG | - | chr4.2:52094735-52094754 | MS.gene62925:intron | 10.0% |
| !!! | TTTTAATGATTATTATTGTT+TGG | - | chr4.2:52093867-52093886 | MS.gene62925:intron | 10.0% |
| !! | TGTAATGATTATAGTTAAAA+GGG | - | chr4.2:52094876-52094895 | MS.gene62925:intron | 15.0% |
| !!! | ACAAGTCTTTTTTAATTATA+CGG | - | chr4.2:52094491-52094510 | MS.gene62925:intron | 15.0% |
| !!! | ATAATTAAAAAAGACTTGTT+TGG | + | chr4.2:52094490-52094509 | None:intergenic | 15.0% |
| !!! | ATATAGCATTTTCTTTATAA+TGG | + | chr4.2:52094984-52095003 | None:intergenic | 15.0% |
| !! | CATTTATATTGAATGCTATT+TGG | - | chr4.2:52095110-52095129 | MS.gene62925:intron | 20.0% |
| !! | CTGTAATGATTATAGTTAAA+AGG | - | chr4.2:52094875-52094894 | MS.gene62925:intron | 20.0% |
| !! | TAACTATAATCATTACAGAA+AGG | + | chr4.2:52094873-52094892 | None:intergenic | 20.0% |
| !! | TCTTTATAATAACCACTATA+AGG | - | chr4.2:52094423-52094442 | MS.gene62925:intron | 20.0% |
| !! | TTATATAGAACTCTTATCAA+AGG | + | chr4.2:52095078-52095097 | None:intergenic | 20.0% |
| !! | TTTGATAAGAGTTCTATATA+AGG | - | chr4.2:52095077-52095096 | MS.gene62925:intron | 20.0% |
| !!! | AACAAAACTTTTCAAGAAAA+AGG | + | chr4.2:52093910-52093929 | None:intergenic | 20.0% |
| !!! | ACAAAACTTTTCAAGAAAAA+GGG | + | chr4.2:52093909-52093928 | None:intergenic | 20.0% |
| !!! | ATTTTTCTCACAACTAAAAT+AGG | + | chr4.2:52093997-52094016 | None:intergenic | 20.0% |
| !!! | CAAGTCTTTTTTAATTATAC+GGG | - | chr4.2:52094492-52094511 | MS.gene62925:intron | 20.0% |
| !!! | TCAATTTCACTCTTTTTTTT+TGG | - | chr4.2:52093841-52093860 | MS.gene62925:intron | 20.0% |
| !!! | TGATTTTCTCAAATTATTAG+CGG | - | chr4.2:52094094-52094113 | MS.gene62925:intron | 20.0% |
| !!! | TTTCTTGAAAAGTTTTGTTT+TGG | - | chr4.2:52093911-52093930 | MS.gene62925:intron | 20.0% |
| ! | AAGCTAGTTATAACTGATAT+CGG | - | chr4.2:52094218-52094237 | MS.gene62925:intron | 25.0% |
| ! | AGAGTTATTAAAAGGTTTGA+AGG | + | chr4.2:52095523-52095542 | None:intergenic | 25.0% |
| ! | AGTTCTATATAAGGTAGAAA+TGG | - | chr4.2:52095086-52095105 | MS.gene62925:intron | 25.0% |
| ! | TAAAAGGGAAATTTCTTTGA+AGG | - | chr4.2:52094891-52094910 | MS.gene62925:intron | 25.0% |
| ! | TCAACCAAAGAGTTATTAAA+AGG | + | chr4.2:52095531-52095550 | None:intergenic | 25.0% |
| ! | TTAATAACTCTTTGGTTGAA+GGG | - | chr4.2:52095532-52095551 | MS.gene62925:CDS | 25.0% |
| ! | TTGATTGAATTTGTAGTGTT+TGG | - | chr4.2:52094250-52094269 | MS.gene62925:intron | 25.0% |
| ! | TTTAATAACTCTTTGGTTGA+AGG | - | chr4.2:52095531-52095550 | MS.gene62925:CDS | 25.0% |
| !! | AAAATTGGTGATTGTTTCAT+TGG | - | chr4.2:52093628-52093647 | MS.gene62925:intron | 25.0% |
| !! | CAAACCTTTTAATAACTCTT+TGG | - | chr4.2:52095524-52095543 | MS.gene62925:CDS | 25.0% |
| !! | GTGTATGTTTTATGGTTAAT+TGG | - | chr4.2:52095482-52095501 | MS.gene62925:CDS | 25.0% |
| !! | TTCAGTGTTATTATATGTGT+CGG | + | chr4.2:52094069-52094088 | None:intergenic | 25.0% |
| !! | TTGTTCTAGTCTTTATAGTA+TGG | - | chr4.2:52093718-52093737 | MS.gene62925:intron | 25.0% |
| !! | TTTCGAATTTTCATGTTTGT+GGG | - | chr4.2:52095241-52095260 | MS.gene62925:intron | 25.0% |
| !!! | AAGTTTTGTTTTGGTGTTTT+TGG | - | chr4.2:52093920-52093939 | MS.gene62925:intron | 25.0% |
| !!! | AGGTATGCACTTTTTTTTTT+TGG | - | chr4.2:52094755-52094774 | MS.gene62925:intron | 25.0% |
| !!! | GGTATGCACTTTTTTTTTTT+GGG | - | chr4.2:52094756-52094775 | MS.gene62925:intron | 25.0% |
| !!! | GTATGCACTTTTTTTTTTTG+GGG | - | chr4.2:52094757-52094776 | MS.gene62925:intron | 25.0% |
| !!! | TTTTTTCAATTAAGCTTGCA+GGG | - | chr4.2:52094340-52094359 | MS.gene62925:intron | 25.0% |
| !!! | TTTTTTTCAATTAAGCTTGC+AGG | - | chr4.2:52094339-52094358 | MS.gene62925:intron | 25.0% |
| AAGACATAAATTTGAGCTCA+TGG | + | chr4.2:52094533-52094552 | None:intergenic | 30.0% | |
| AAGATTAATGGAATTGGCAT+AGG | + | chr4.2:52095440-52095459 | None:intergenic | 30.0% | |
| AAGTTACCTTCTATGAAACA+CGG | + | chr4.2:52094167-52094186 | None:intergenic | 30.0% | |
| ACACTACAAATTCAATCAAC+TGG | + | chr4.2:52094249-52094268 | None:intergenic | 30.0% | |
| ACAGTGATTGATTGATTGAT+TGG | - | chr4.2:52095170-52095189 | MS.gene62925:intron | 30.0% | |
| ACATAAGAACTTGATCAGTA+GGG | + | chr4.2:52093784-52093803 | None:intergenic | 30.0% | |
| AGAAGATGAATTGAGAAAGA+TGG | - | chr4.2:52095021-52095040 | MS.gene62925:intron | 30.0% | |
| AGCAATCACTTATATACTTG+TGG | + | chr4.2:52095382-52095401 | None:intergenic | 30.0% | |
| ATAATAACCACTATAAGGTC+TGG | - | chr4.2:52094428-52094447 | MS.gene62925:intron | 30.0% | |
| ATGCTTGAAGCTTCTTTAAT+GGG | - | chr4.2:52093812-52093831 | MS.gene62925:intron | 30.0% | |
| ATTCGAAGCAACTGAAAAAT+AGG | + | chr4.2:52094833-52094852 | None:intergenic | 30.0% | |
| CATAGATCAGGAAAGATTAA+TGG | + | chr4.2:52095452-52095471 | None:intergenic | 30.0% | |
| GTAGTGTTTGGTAAAATTAG+TGG | - | chr4.2:52094262-52094281 | MS.gene62925:intron | 30.0% | |
| TAATAACTCTTTGGTTGAAG+GGG | - | chr4.2:52095533-52095552 | MS.gene62925:CDS | 30.0% | |
| TACAATGTCATAAAAGTCAG+AGG | + | chr4.2:52095147-52095166 | None:intergenic | 30.0% | |
| TATGCTTGAAGCTTCTTTAA+TGG | - | chr4.2:52093811-52093830 | MS.gene62925:intron | 30.0% | |
| TCAAATGACTCAATATTTCG+TGG | - | chr4.2:52095214-52095233 | MS.gene62925:intron | 30.0% | |
| TCAGGAAAGATTAATGGAAT+TGG | + | chr4.2:52095446-52095465 | None:intergenic | 30.0% | |
| TTTCTCCTAAGAATCTGTTT+GGG | + | chr4.2:52094557-52094576 | None:intergenic | 30.0% | |
| ! | ATCATGCAGTGTATGTTTTA+TGG | - | chr4.2:52095474-52095493 | MS.gene62925:CDS | 30.0% |
| ! | GTTTCGAATTTTCATGTTTG+TGG | - | chr4.2:52095240-52095259 | MS.gene62925:intron | 30.0% |
| ! | TTACTTTACACAGCGATTTT+CGG | - | chr4.2:52093560-52093579 | MS.gene62925:CDS | 30.0% |
| ! | TTATTTTCCAGACCTTATAG+TGG | + | chr4.2:52094438-52094457 | None:intergenic | 30.0% |
| ! | TTTTCTCCTAAGAATCTGTT+TGG | + | chr4.2:52094558-52094577 | None:intergenic | 30.0% |
| !! | AATTAGCTTGATAGTGTTGT+TGG | - | chr4.2:52094917-52094936 | MS.gene62925:intron | 30.0% |
| !! | ATGTTTGTGGGTTATTGTTT+TGG | - | chr4.2:52095253-52095272 | MS.gene62925:intron | 30.0% |
| !! | TTTTTCAATTAAGCTTGCAG+GGG | - | chr4.2:52094341-52094360 | MS.gene62925:intron | 30.0% |
| GACATAAGAACTTGATCAGT+AGG | + | chr4.2:52093785-52093804 | None:intergenic | 35.0% | |
| GACGAAAAACATATCGAGTT+TGG | + | chr4.2:52094784-52094803 | None:intergenic | 35.0% | |
| GTTATAAGCACGTGTAAAAC+TGG | - | chr4.2:52094458-52094477 | MS.gene62925:intron | 35.0% | |
| TATAGCTAACGAGCTTATAG+CGG | - | chr4.2:52094186-52094205 | MS.gene62925:intron | 35.0% | |
| TCTTACCCAAACAGATTCTT+AGG | - | chr4.2:52094549-52094568 | MS.gene62925:intron | 35.0% | |
| ! | AATCACCAATTTTCGAACTG+GGG | + | chr4.2:52093621-52093640 | None:intergenic | 35.0% |
| ! | ACAATCACCAATTTTCGAAC+TGG | + | chr4.2:52093623-52093642 | None:intergenic | 35.0% |
| ! | CAATCACCAATTTTCGAACT+GGG | + | chr4.2:52093622-52093641 | None:intergenic | 35.0% |
| ! | GCTATAAGCTTTTAAGCAAC+AGG | - | chr4.2:52094388-52094407 | MS.gene62925:intron | 35.0% |
| !! | GTCGGTGTTATTATATGTGT+CGG | + | chr4.2:52094051-52094070 | None:intergenic | 35.0% |
| !! | GTTGGCTATGATTTGATTTG+TGG | - | chr4.2:52094935-52094954 | MS.gene62925:intron | 35.0% |
| !!! | AACTGGGGTTTTGAAAACTA+GGG | + | chr4.2:52093606-52093625 | None:intergenic | 35.0% |
| !!! | AGGAATAAGTTTTTGAGGCA+AGG | - | chr4.2:52094622-52094641 | MS.gene62925:intron | 35.0% |
| !!! | GTTATTGTTTTGGTTGGGAA+TGG | - | chr4.2:52095263-52095282 | MS.gene62925:intron | 35.0% |
| !!! | TGTGGGTTATTGTTTTGGTT+GGG | - | chr4.2:52095258-52095277 | MS.gene62925:intron | 35.0% |
| !!! | TTGTGGGTTATTGTTTTGGT+TGG | - | chr4.2:52095257-52095276 | MS.gene62925:intron | 35.0% |
| ACTGCATGATCACATAGATC+AGG | + | chr4.2:52095464-52095483 | None:intergenic | 40.0% | |
| AGGGAGTGACTATATTGTCA+TGG | + | chr4.2:52094588-52094607 | None:intergenic | 40.0% | |
| ATTCCTCGCCTCTTAAAAGA+AGG | + | chr4.2:52094608-52094627 | None:intergenic | 40.0% | |
| CAAAACCCCAGTTCGAAAAT+TGG | - | chr4.2:52093613-52093632 | MS.gene62925:intron | 40.0% | |
| CCAACTGTAGTAACAACGAT+TGG | + | chr4.2:52093677-52093696 | None:intergenic | 40.0% | |
| CCAATCGTTGTTACTACAGT+TGG | - | chr4.2:52093674-52093693 | MS.gene62925:intron | 40.0% | |
| CCTTCTATGAAACACGGATA+CGG | + | chr4.2:52094161-52094180 | None:intergenic | 40.0% | |
| GCAAAGATATTGCTCATCTC+AGG | + | chr4.2:52095418-52095437 | None:intergenic | 40.0% | |
| TCATTGGATCGAGTCAAAAG+AGG | - | chr4.2:52093644-52093663 | MS.gene62925:intron | 40.0% | |
| TTCCTCGCCTCTTAAAAGAA+GGG | + | chr4.2:52094607-52094626 | None:intergenic | 40.0% | |
| ! | AGGCGAGGAATAAGTTTTTG+AGG | - | chr4.2:52094617-52094636 | MS.gene62925:intron | 40.0% |
| ! | AGTCACTCCCTTCTTTTAAG+AGG | - | chr4.2:52094597-52094616 | MS.gene62925:intron | 40.0% |
| ! | GGGGTGTTTGATTCAAAAGT+TGG | - | chr4.2:52095552-52095571 | MS.gene62925:CDS | 40.0% |
| ! | TGTGAATGTTTGACTTACGC+TGG | - | chr4.2:52095317-52095336 | MS.gene62925:CDS | 40.0% |
| !!! | GAACTGGGGTTTTGAAAACT+AGG | + | chr4.2:52093607-52093626 | None:intergenic | 40.0% |
| !!! | GGGTTTTGAAAACTAGGGTT+AGG | + | chr4.2:52093601-52093620 | None:intergenic | 40.0% |
| ATGAAACACGGATACGGACA+CGG | + | chr4.2:52094155-52094174 | None:intergenic | 45.0% | |
| CCGTATCCGTGTTTCATAGA+AGG | - | chr4.2:52094158-52094177 | MS.gene62925:intron | 45.0% | |
| GGCTAAGATCCAACAACGTA+CGG | - | chr4.2:52093739-52093758 | MS.gene62925:intron | 45.0% | |
| ! | GGCAAGGACTAATTTGTCGA+TGG | - | chr4.2:52094638-52094657 | MS.gene62925:intron | 45.0% |
| ACACGACATGGACACTGACA+CGG | + | chr4.2:52094125-52094144 | None:intergenic | 50.0% | |
| CAACAACGTACGGTTGAAGC+TGG | - | chr4.2:52093749-52093768 | MS.gene62925:intron | 50.0% | |
| CAGCTTCAACCGTACGTTGT+TGG | + | chr4.2:52093751-52093770 | None:intergenic | 50.0% | |
| GATCGAGTCAAAAGAGGTCG+TGG | - | chr4.2:52093650-52093669 | MS.gene62925:intron | 50.0% | |
| GGGAATGGTTGAGTGTGTCT+TGG | - | chr4.2:52095278-52095297 | MS.gene62925:intron | 50.0% | |
| GGTTGAGTGTGTCTTGGAGT+GGG | - | chr4.2:52095284-52095303 | MS.gene62925:intron | 50.0% | |
| GTTGAGTGTGTCTTGGAGTG+GGG | - | chr4.2:52095285-52095304 | MS.gene62925:intron | 50.0% | |
| TGAAACACGGATACGGACAC+GGG | + | chr4.2:52094154-52094173 | None:intergenic | 50.0% | |
| TGGTTGAGTGTGTCTTGGAG+TGG | - | chr4.2:52095283-52095302 | MS.gene62925:intron | 50.0% | |
| !! | CTCCCTTCTTTTAAGAGGCG+AGG | - | chr4.2:52094602-52094621 | MS.gene62925:intron | 50.0% |
| GTCAGTGTCCATGTCGTGTC+CGG | - | chr4.2:52094126-52094145 | MS.gene62925:intron | 55.0% | |
| ACGGATACGGACACGGGTAC+CGG | + | chr4.2:52094148-52094167 | None:intergenic | 60.0% | |
| !! | CACGGGTACCGGACACGACA+TGG | + | chr4.2:52094137-52094156 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 52093551 | 52095578 | 52093551 | ID=MS.gene62925 |
| chr4.2 | mRNA | 52093551 | 52095578 | 52093551 | ID=MS.gene62925.t1;Parent=MS.gene62925 |
| chr4.2 | exon | 52095296 | 52095578 | 52095296 | ID=MS.gene62925.t1.exon1;Parent=MS.gene62925.t1 |
| chr4.2 | CDS | 52095296 | 52095578 | 52095296 | ID=cds.MS.gene62925.t1;Parent=MS.gene62925.t1 |
| chr4.2 | exon | 52093551 | 52093612 | 52093551 | ID=MS.gene62925.t1.exon2;Parent=MS.gene62925.t1 |
| chr4.2 | CDS | 52093551 | 52093612 | 52093551 | ID=cds.MS.gene62925.t1;Parent=MS.gene62925.t1 |
| Gene Sequence |
| Protein sequence |
