Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62936.t1 | XP_003588667.1 | 99.5 | 184 | 1 | 0 | 1 | 184 | 1 | 184 | 1.10E-94 | 355.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62936.t1 | Q6NQH9 | 38.5 | 182 | 93 | 4 | 2 | 181 | 1 | 165 | 7.2e-18 | 92.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62936.t1 | G7I5S9 | 99.5 | 184 | 1 | 0 | 1 | 184 | 1 | 184 | 8.0e-95 | 355.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050379 | MS.gene62936 | 0.817216 | 3.70E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62936.t1 | MTR_1g011280 | 99.457 | 184 | 1 | 0 | 1 | 184 | 1 | 184 | 2.12e-135 | 376 |
| MS.gene62936.t1 | MTR_1g011280 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 4.05e-98 | 280 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62936.t1 | AT5G25540 | 39.306 | 173 | 86 | 5 | 2 | 172 | 1 | 156 | 1.48e-24 | 94.7 |
| MS.gene62936.t1 | AT5G25540 | 39.306 | 173 | 86 | 5 | 2 | 172 | 1 | 156 | 1.48e-24 | 94.7 |
| MS.gene62936.t1 | AT5G11440 | 38.172 | 186 | 69 | 7 | 2 | 181 | 1 | 146 | 9.05e-24 | 92.0 |
| MS.gene62936.t1 | AT5G11440 | 38.172 | 186 | 69 | 7 | 2 | 181 | 1 | 146 | 9.05e-24 | 92.0 |
| MS.gene62936.t1 | AT5G25540 | 37.956 | 137 | 71 | 3 | 2 | 136 | 1 | 125 | 7.01e-18 | 76.3 |
Find 40 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATATTGATTTGGAATATCTT+AGG | 0.249147 | 1.1:-9357376 | MS.gene62936:CDS |
| GCAGAATCAGCTGGCATAAC+AGG | 0.299732 | 1.1:+9356127 | None:intergenic |
| GAAGATTCCAAGGACTATAA+TGG | 0.336500 | 1.1:-9357569 | MS.gene62936:CDS |
| TGAAGAGCTTGATATTGATT+TGG | 0.356150 | 1.1:-9357387 | MS.gene62936:CDS |
| TCTAACATTAGTGTCCCTTT+TGG | 0.366851 | 1.1:+9357604 | None:intergenic |
| CTTCCAGAGTCGCTGGATAT+TGG | 0.369329 | 1.1:-9356163 | MS.gene62936:CDS |
| TATCTTAGGATGACATTTCC+TGG | 0.384113 | 1.1:-9357362 | MS.gene62936:CDS |
| AAGATTCCAAGGACTATAAT+GGG | 0.425801 | 1.1:-9357568 | MS.gene62936:CDS |
| GCTGACAGAGAATCAGTATA+TGG | 0.429347 | 1.1:-9357411 | MS.gene62936:CDS |
| GACAAGTTGCACATCATTTG+TGG | 0.436093 | 1.1:+9357517 | None:intergenic |
| TACAGTCCCATTATAGTCCT+TGG | 0.442789 | 1.1:+9357562 | None:intergenic |
| GGAACGTACGCAGCTGCATA+TGG | 0.448700 | 1.1:+9357632 | None:intergenic |
| CGTACGTTCCTATGGCCAAA+AGG | 0.452015 | 1.1:-9357619 | MS.gene62936:CDS |
| CAATGTAAACAGTGGTGACT+TGG | 0.452279 | 1.1:-9357309 | MS.gene62936:CDS |
| AACGCTCAAAGGCTCTCTCC+GGG | 0.456586 | 1.1:-9357488 | MS.gene62936:CDS |
| GAACGCTCAAAGGCTCTCTC+CGG | 0.458973 | 1.1:-9357489 | MS.gene62936:CDS |
| ACATCATTTGTGGTATATTG+AGG | 0.475780 | 1.1:+9357527 | None:intergenic |
| TCACCAATATCCAGCGACTC+TGG | 0.486657 | 1.1:+9356160 | None:intergenic |
| TGCAGCTGCGTACGTTCCTA+TGG | 0.491027 | 1.1:-9357627 | MS.gene62936:CDS |
| AGGACTATAATGGGACTGTA+TGG | 0.501746 | 1.1:-9357559 | MS.gene62936:CDS |
| TTTAAGATTATACCTCAAGT+TGG | 0.519886 | 1.1:+9357264 | None:intergenic |
| TGGAAGTCTTCCAGAGTCGC+TGG | 0.520324 | 1.1:-9356170 | MS.gene62936:CDS |
| AAACAGAAGAATGCAACAGC+TGG | 0.520914 | 1.1:-9356097 | MS.gene62936:CDS |
| CAAGTGATGAGATGAAGTAC+TGG | 0.526656 | 1.1:+9356072 | None:intergenic |
| GAAAAGTAAGAGCTCGAAGC+AGG | 0.531344 | 1.1:+9357455 | None:intergenic |
| TTTGATGAAGCAGAATCAGC+TGG | 0.546972 | 1.1:+9356118 | None:intergenic |
| AGTAAGAGCTCGAAGCAGGC+TGG | 0.559139 | 1.1:+9357459 | None:intergenic |
| GTACGTTCCTATGGCCAAAA+GGG | 0.559981 | 1.1:-9357618 | MS.gene62936:CDS |
| GGATTCAAAGAAGACTTTCG+CGG | 0.573737 | 1.1:+9357653 | None:intergenic |
| GATGATGCTGTTGACGCTCA+TGG | 0.575449 | 1.1:-9356190 | MS.gene62936:CDS |
| TTGTCGAGAAGAACGCTCAA+AGG | 0.582696 | 1.1:-9357499 | MS.gene62936:CDS |
| TTACATTGTAGACATCTACA+AGG | 0.595095 | 1.1:+9357324 | None:intergenic |
| TCATTTGTGGTATATTGAGG+AGG | 0.614473 | 1.1:+9357530 | None:intergenic |
| TTATGTGACAGAAGATTCCA+AGG | 0.616516 | 1.1:-9357579 | MS.gene62936:CDS |
| GTCAGCTGTGCCACACTCTG+TGG | 0.634116 | 1.1:+9357428 | None:intergenic |
| TGAGATGCTAAGCCAACTTG+AGG | 0.661491 | 1.1:-9357276 | MS.gene62936:intron |
| TACATTGTAGACATCTACAA+GGG | 0.670208 | 1.1:+9357325 | None:intergenic |
| AGGGACTCATCAGATATACC+AGG | 0.671666 | 1.1:+9357344 | None:intergenic |
| ACGCTCAAAGGCTCTCTCCG+GGG | 0.694097 | 1.1:-9357487 | MS.gene62936:CDS |
| GATGTCTACAATGTAAACAG+TGG | 0.761880 | 1.1:-9357317 | MS.gene62936:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATATAATTGTAACTATCA+AGG | - | chr1.1:9356766-9356785 | MS.gene62936:intron | 15.0% |
| !! | ATATAAATATCAAGACAATA+TGG | + | chr1.1:9357029-9357048 | None:intergenic | 15.0% |
| !!! | ATCTTAAAATTATTTGCTTT+TGG | - | chr1.1:9356482-9356501 | MS.gene62936:intron | 15.0% |
| !!! | TCTTAAAATTATTTGCTTTT+GGG | - | chr1.1:9356483-9356502 | MS.gene62936:intron | 15.0% |
| !! | AAAAACAAACTCTCAAAAAT+TGG | + | chr1.1:9357506-9357525 | None:intergenic | 20.0% |
| !! | TGATTGGAATATATGAAAAA+AGG | + | chr1.1:9356534-9356553 | None:intergenic | 20.0% |
| !! | TTATATATGCAGATGAAAAT+AGG | - | chr1.1:9357284-9357303 | MS.gene62936:CDS | 20.0% |
| !! | TTATATTTATAAATCCCGAA+TGG | + | chr1.1:9356754-9356773 | None:intergenic | 20.0% |
| !!! | ATATTGATTTGGAATATCTT+AGG | - | chr1.1:9356355-9356374 | MS.gene62936:intron | 20.0% |
| ! | AATAAGAAGTTATCATCTTG+TGG | - | chr1.1:9356936-9356955 | MS.gene62936:intron | 25.0% |
| ! | TACTCTCATAATCATGTTAA+TGG | - | chr1.1:9357450-9357469 | MS.gene62936:CDS | 25.0% |
| !! | CTGATTTGAATCTAGTATAT+AGG | - | chr1.1:9357231-9357250 | MS.gene62936:intron | 25.0% |
| !! | TTTAAGATTATACCTCAAGT+TGG | + | chr1.1:9356470-9356489 | None:intergenic | 25.0% |
| !!! | ACATGTTCAGTTTTTGTTTT+AGG | - | chr1.1:9357179-9357198 | MS.gene62936:intron | 25.0% |
| AAAACAAACATACCAGGAAA+TGG | + | chr1.1:9357067-9357086 | None:intergenic | 30.0% | |
| AAACAAACATACCAGGAAAT+GGG | + | chr1.1:9357066-9357085 | None:intergenic | 30.0% | |
| AAGATTCCAAGGACTATAAT+GGG | - | chr1.1:9356163-9356182 | MS.gene62936:CDS | 30.0% | |
| ACATCATTTGTGGTATATTG+AGG | + | chr1.1:9356207-9356226 | None:intergenic | 30.0% | |
| AGTCTTAAAACAAACATACC+AGG | + | chr1.1:9357073-9357092 | None:intergenic | 30.0% | |
| ATAAAAGTCACTGAACTGAT+TGG | + | chr1.1:9356651-9356670 | None:intergenic | 30.0% | |
| ATTACAGTCACTTAACTGAT+TGG | + | chr1.1:9356550-9356569 | None:intergenic | 30.0% | |
| ATTGACTGGAAATTATGGTT+AGG | - | chr1.1:9356812-9356831 | MS.gene62936:intron | 30.0% | |
| CACAAATTGACTGGAAATTA+TGG | - | chr1.1:9356807-9356826 | MS.gene62936:intron | 30.0% | |
| CATAATTTCCAGTCAATTTG+TGG | + | chr1.1:9356809-9356828 | None:intergenic | 30.0% | |
| GACTGTAATTGTAACTATCA+AGG | - | chr1.1:9356561-9356580 | MS.gene62936:intron | 30.0% | |
| TACATTGTAGACATCTACAA+GGG | + | chr1.1:9356409-9356428 | None:intergenic | 30.0% | |
| TTACATTGTAGACATCTACA+AGG | + | chr1.1:9356410-9356429 | None:intergenic | 30.0% | |
| TTAGGATGTGTAGTTAAATG+TGG | - | chr1.1:9356830-9356849 | MS.gene62936:intron | 30.0% | |
| TTTGACTGGAAAATTTGGTT+AGG | - | chr1.1:9356607-9356626 | MS.gene62936:intron | 30.0% | |
| ! | ACTTCTTTGCTGATTAATGT+TGG | - | chr1.1:9357331-9357350 | MS.gene62936:CDS | 30.0% |
| ! | TCAGTTCAGTGACTTTTATT+CGG | - | chr1.1:9356652-9356671 | MS.gene62936:intron | 30.0% |
| ! | TGAAGAGCTTGATATTGATT+TGG | - | chr1.1:9356344-9356363 | MS.gene62936:intron | 30.0% |
| ! | TTCGATTTGACTGGAAAATT+TGG | - | chr1.1:9356602-9356621 | MS.gene62936:intron | 30.0% |
| !!! | GGTATGTTTGTTTTAAGACT+TGG | - | chr1.1:9357073-9357092 | MS.gene62936:intron | 30.0% |
| !!! | TGTTCAGTTTTTGTTTTAGG+TGG | - | chr1.1:9357182-9357201 | MS.gene62936:intron | 30.0% |
| !!! | TTTAAGACTTGGTGTTTTTC+TGG | - | chr1.1:9357084-9357103 | MS.gene62936:intron | 30.0% |
| AGACTATCTTCAGATGACAT+TGG | - | chr1.1:9356909-9356928 | MS.gene62936:intron | 35.0% | |
| ATCATATGCCACAAATTGAC+TGG | - | chr1.1:9356798-9356817 | MS.gene62936:intron | 35.0% | |
| CTGGAAAATTTGGTTAGGTT+AGG | - | chr1.1:9356612-9356631 | MS.gene62936:intron | 35.0% | |
| GAAGATTCCAAGGACTATAA+TGG | - | chr1.1:9356162-9356181 | MS.gene62936:CDS | 35.0% | |
| GATGTCTACAATGTAAACAG+TGG | - | chr1.1:9356414-9356433 | MS.gene62936:intron | 35.0% | |
| TATCTTAGGATGACATTTCC+TGG | - | chr1.1:9356369-9356388 | MS.gene62936:intron | 35.0% | |
| TCATTTGTGGTATATTGAGG+AGG | + | chr1.1:9356204-9356223 | None:intergenic | 35.0% | |
| TGACTAGACTCTCATACATA+TGG | + | chr1.1:9356730-9356749 | None:intergenic | 35.0% | |
| TGTCATCTGAAGATAGTCTT+CGG | + | chr1.1:9356908-9356927 | None:intergenic | 35.0% | |
| TTATGTGACAGAAGATTCCA+AGG | - | chr1.1:9356152-9356171 | MS.gene62936:CDS | 35.0% | |
| TTTGCGTAGAGAGAAAACTT+TGG | - | chr1.1:9357422-9357441 | MS.gene62936:CDS | 35.0% | |
| ! | AACCACAAAGCATTACACAT+GGG | + | chr1.1:9357130-9357149 | None:intergenic | 35.0% |
| ! | ATCATATGCTTCGATTTGAC+TGG | - | chr1.1:9356593-9356612 | MS.gene62936:intron | 35.0% |
| ! | CAGTTCAGTGACTTTTATTC+GGG | - | chr1.1:9356653-9356672 | MS.gene62936:intron | 35.0% |
| ! | GAAAAGAGTATACACAGGAA+GGG | + | chr1.1:9356880-9356899 | None:intergenic | 35.0% |
| ! | TCTAACATTAGTGTCCCTTT+TGG | + | chr1.1:9356130-9356149 | None:intergenic | 35.0% |
| !!! | CAGTTTTTGTTTTAGGTGGA+AGG | - | chr1.1:9357186-9357205 | MS.gene62936:intron | 35.0% |
| AAACAGAAGAATGCAACAGC+TGG | - | chr1.1:9357634-9357653 | MS.gene62936:CDS | 40.0% | |
| CAAGTGATGAGATGAAGTAC+TGG | + | chr1.1:9357662-9357681 | None:intergenic | 40.0% | |
| CAATGTAAACAGTGGTGACT+TGG | - | chr1.1:9356422-9356441 | MS.gene62936:intron | 40.0% | |
| GACAAGTTGCACATCATTTG+TGG | + | chr1.1:9356217-9356236 | None:intergenic | 40.0% | |
| GCTGACAGAGAATCAGTATA+TGG | - | chr1.1:9356320-9356339 | MS.gene62936:intron | 40.0% | |
| GGATTCAAAGAAGACTTTCG+CGG | + | chr1.1:9356081-9356100 | None:intergenic | 40.0% | |
| GTCATCTGAAGATAGTCTTC+GGG | + | chr1.1:9356907-9356926 | None:intergenic | 40.0% | |
| TACAGTCCCATTATAGTCCT+TGG | + | chr1.1:9356172-9356191 | None:intergenic | 40.0% | |
| TGAGAGTCTAGTCAACCATT+CGG | - | chr1.1:9356736-9356755 | MS.gene62936:intron | 40.0% | |
| TGTGGTTGTTGAATCTCACA+GGG | - | chr1.1:9356954-9356973 | MS.gene62936:intron | 40.0% | |
| TTCCCATGTGTAATGCTTTG+TGG | - | chr1.1:9357125-9357144 | MS.gene62936:intron | 40.0% | |
| TTGTGGTTGTTGAATCTCAC+AGG | - | chr1.1:9356953-9356972 | MS.gene62936:intron | 40.0% | |
| TTTGATGAAGCAGAATCAGC+TGG | + | chr1.1:9357616-9357635 | None:intergenic | 40.0% | |
| ! | AGGACTATAATGGGACTGTA+TGG | - | chr1.1:9356172-9356191 | MS.gene62936:CDS | 40.0% |
| ! | GAACCACAAAGCATTACACA+TGG | + | chr1.1:9357131-9357150 | None:intergenic | 40.0% |
| ! | GAACGGAAAAGAGTATACAC+AGG | + | chr1.1:9356885-9356904 | None:intergenic | 40.0% |
| ! | GGAAAAGAGTATACACAGGA+AGG | + | chr1.1:9356881-9356900 | None:intergenic | 40.0% |
| ! | TTTTGTGCATTCCCATTTCC+TGG | - | chr1.1:9357052-9357071 | MS.gene62936:intron | 40.0% |
| AGGGACTCATCAGATATACC+AGG | + | chr1.1:9356390-9356409 | None:intergenic | 45.0% | |
| CTGAAGATAGTCTTCGGGAA+CGG | + | chr1.1:9356902-9356921 | None:intergenic | 45.0% | |
| GAAAAGTAAGAGCTCGAAGC+AGG | + | chr1.1:9356279-9356298 | None:intergenic | 45.0% | |
| GAGAGTCTAGTCAACCATTC+GGG | - | chr1.1:9356737-9356756 | MS.gene62936:intron | 45.0% | |
| GTACGTTCCTATGGCCAAAA+GGG | - | chr1.1:9356113-9356132 | MS.gene62936:CDS | 45.0% | |
| TGAGATGCTAAGCCAACTTG+AGG | - | chr1.1:9356455-9356474 | MS.gene62936:intron | 45.0% | |
| TTGGTCGTTCATTCAGTTGC+TGG | - | chr1.1:9357350-9357369 | MS.gene62936:CDS | 45.0% | |
| TTGTCGAGAAGAACGCTCAA+AGG | - | chr1.1:9356232-9356251 | MS.gene62936:intron | 45.0% | |
| ! | CTTTTCATCACCACAGAGTG+TGG | - | chr1.1:9356293-9356312 | MS.gene62936:intron | 45.0% |
| ! | TTAGTGTCCCTTTTGGCCAT+AGG | + | chr1.1:9356123-9356142 | None:intergenic | 45.0% |
| CGTACGTTCCTATGGCCAAA+AGG | - | chr1.1:9356112-9356131 | MS.gene62936:CDS | 50.0% | |
| CTTCCAGAGTCGCTGGATAT+TGG | - | chr1.1:9357568-9357587 | MS.gene62936:CDS | 50.0% | |
| GATGATGCTGTTGACGCTCA+TGG | - | chr1.1:9357541-9357560 | MS.gene62936:CDS | 50.0% | |
| GCAGAATCAGCTGGCATAAC+AGG | + | chr1.1:9357607-9357626 | None:intergenic | 50.0% | |
| TCACCAATATCCAGCGACTC+TGG | + | chr1.1:9357574-9357593 | None:intergenic | 50.0% | |
| TGTTGAATCTCACAGGGTGC+TGG | - | chr1.1:9356960-9356979 | MS.gene62936:intron | 50.0% | |
| AACGCTCAAAGGCTCTCTCC+GGG | - | chr1.1:9356243-9356262 | MS.gene62936:intron | 55.0% | |
| AGTAAGAGCTCGAAGCAGGC+TGG | + | chr1.1:9356275-9356294 | None:intergenic | 55.0% | |
| GAACGCTCAAAGGCTCTCTC+CGG | - | chr1.1:9356242-9356261 | MS.gene62936:intron | 55.0% | |
| GGAACGTACGCAGCTGCATA+TGG | + | chr1.1:9356102-9356121 | None:intergenic | 55.0% | |
| TGCAGCTGCGTACGTTCCTA+TGG | - | chr1.1:9356104-9356123 | MS.gene62936:CDS | 55.0% | |
| TGGAAGTCTTCCAGAGTCGC+TGG | - | chr1.1:9357561-9357580 | MS.gene62936:CDS | 55.0% | |
| !! | AATCTCACAGGGTGCTGGCT+AGG | - | chr1.1:9356965-9356984 | MS.gene62936:intron | 55.0% |
| !!! | GAAGCAGGCTGGTTTTTCCC+CGG | + | chr1.1:9356264-9356283 | None:intergenic | 55.0% |
| ACGCTCAAAGGCTCTCTCCG+GGG | - | chr1.1:9356244-9356263 | MS.gene62936:intron | 60.0% | |
| GTCAGCTGTGCCACACTCTG+TGG | + | chr1.1:9356306-9356325 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 9356069 | 9357684 | 9356069 | ID=MS.gene62936 |
| chr1.1 | mRNA | 9356069 | 9357684 | 9356069 | ID=MS.gene62936.t1;Parent=MS.gene62936 |
| chr1.1 | exon | 9357277 | 9357684 | 9357277 | ID=MS.gene62936.t1.exon1;Parent=MS.gene62936.t1 |
| chr1.1 | CDS | 9357277 | 9357684 | 9357277 | ID=cds.MS.gene62936.t1;Parent=MS.gene62936.t1 |
| chr1.1 | exon | 9356069 | 9356215 | 9356069 | ID=MS.gene62936.t1.exon2;Parent=MS.gene62936.t1 |
| chr1.1 | CDS | 9356069 | 9356215 | 9356069 | ID=cds.MS.gene62936.t1;Parent=MS.gene62936.t1 |
| Gene Sequence |
| Protein sequence |
