Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63358.t1 | XP_013460646.1 | 97.7 | 1107 | 26 | 0 | 1 | 1107 | 1 | 1107 | 0.00E+00 | 2184.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63358.t1 | Q8RY22 | 71.7 | 1112 | 289 | 10 | 1 | 1102 | 1 | 1096 | 0.0e+00 | 1577.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63358.t1 | A0A072V916 | 97.7 | 1107 | 26 | 0 | 1 | 1107 | 1 | 1107 | 0.0e+00 | 2184.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049824 | MS.gene63358 | 0.801831 | 7.48E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene63358 | 0.800946 | 1.14E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63358.t1 | MTR_3g067695 | 97.651 | 1107 | 26 | 0 | 1 | 1107 | 1 | 1107 | 0.0 | 2232 |
| MS.gene63358.t1 | MTR_3g067690 | 87.226 | 1096 | 138 | 2 | 13 | 1107 | 17 | 1111 | 0.0 | 1943 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63358.t1 | AT3G03380 | 71.608 | 1113 | 290 | 10 | 1 | 1103 | 1 | 1097 | 0.0 | 1573 |
| MS.gene63358.t1 | AT3G03380 | 70.901 | 1110 | 270 | 8 | 1 | 1103 | 1 | 1064 | 0.0 | 1560 |
| MS.gene63358.t1 | AT3G03370 | 75.000 | 40 | 10 | 0 | 424 | 463 | 43 | 82 | 4.21e-11 | 63.9 |
Find 300 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTCTAGGTACATGATTT+TGG | 0.045195 | 3.3:+59597262 | MS.gene63358:intron |
| AATGAGGGTCATGGTGTATT+TGG | 0.161168 | 3.3:+59613195 | MS.gene63358:CDS |
| GTTCATTCACAGTCCTTTAT+TGG | 0.170426 | 3.3:+59607770 | MS.gene63358:CDS |
| ACTAACTTCCAGGAAATAAT+TGG | 0.171850 | 3.3:-59604913 | None:intergenic |
| GAGTCTCATCAAACCCTTTA+TGG | 0.180402 | 3.3:-59600832 | None:intergenic |
| CGTAAAACCTGTCGTCTTAT+TGG | 0.182040 | 3.3:-59612744 | None:intergenic |
| AGGGCAGTGGCCCTGAATGC+TGG | 0.219688 | 3.3:+59599453 | MS.gene63358:CDS |
| AGGAGATTCTGTTTACCTTT+TGG | 0.234395 | 3.3:+59608905 | MS.gene63358:CDS |
| TTAATCCAGACACTGGAATT+TGG | 0.255950 | 3.3:+59614930 | MS.gene63358:CDS |
| AAGGAAGGGTCCGAGCTTTA+TGG | 0.256017 | 3.3:+59609181 | MS.gene63358:CDS |
| CCATCTGCTTTGGGACCTAT+TGG | 0.266489 | 3.3:+59608273 | MS.gene63358:CDS |
| CAAGCAAAGTCTCCAGTTTC+AGG | 0.271572 | 3.3:-59602443 | None:intergenic |
| GGGTCACCTCTTCTCATAAA+TGG | 0.274771 | 3.3:+59611353 | MS.gene63358:CDS |
| GCAAATCTTGCTTCAATGTT+AGG | 0.275294 | 3.3:-59614880 | None:intergenic |
| GTGAAACTTATGTTGATAAA+TGG | 0.279401 | 3.3:+59599641 | MS.gene63358:CDS |
| GCTTCTCCTGCCACTGAAAC+TGG | 0.280010 | 3.3:+59601002 | MS.gene63358:CDS |
| ACTAAGTGATGTTTGGATTC+AGG | 0.287605 | 3.3:+59611457 | MS.gene63358:CDS |
| CGTTCACCAGTCAATGAAAT+TGG | 0.289969 | 3.3:-59606857 | None:intergenic |
| ATCAAAGGCTGAAAATCTTT+TGG | 0.292232 | 3.3:+59612788 | MS.gene63358:CDS |
| CGTCATCCAGGTTTCCATCT+TGG | 0.294532 | 3.3:+59597944 | MS.gene63358:intron |
| TGTCATCCCGAGTGTATATC+TGG | 0.299632 | 3.3:-59606742 | None:intergenic |
| CTACCTTGCCAATCTATAAC+TGG | 0.305101 | 3.3:-59599432 | None:intergenic |
| GAAGCCTTCGAGCAACTTCT+AGG | 0.305522 | 3.3:+59608937 | MS.gene63358:CDS |
| AATATATCACACTCAAGATA+TGG | 0.312515 | 3.3:+59607805 | MS.gene63358:CDS |
| CAATTCACAGGTGGAGTTAT+GGG | 0.321808 | 3.3:+59614254 | MS.gene63358:intron |
| GCACAACTCGTACAGTTAAT+TGG | 0.324113 | 3.3:+59613115 | MS.gene63358:CDS |
| CTCACTGGATCTCTGTATAT+TGG | 0.325069 | 3.3:-59595991 | None:intergenic |
| GCTTTCCCAGTTGAAATTCC+TGG | 0.327424 | 3.3:+59607893 | MS.gene63358:CDS |
| GGGCAGTGGCCCTGAATGCT+GGG | 0.330700 | 3.3:+59599454 | MS.gene63358:CDS |
| ACCTCTCCAGGAATTTCAAC+TGG | 0.334389 | 3.3:-59607899 | None:intergenic |
| CGTGCTGCAGAATTACTTCC+AGG | 0.341105 | 3.3:+59608309 | MS.gene63358:CDS |
| TAGCCCCAGATGATAGAAAA+AGG | 0.341781 | 3.3:-59606808 | None:intergenic |
| AATAACTCCCAATTATTTCC+TGG | 0.342899 | 3.3:+59604905 | MS.gene63358:CDS |
| GGCCGGTACTATTGCTCGTT+TGG | 0.343918 | 3.3:+59597965 | MS.gene63358:CDS |
| CTTTCAAAGGCTCGTAGTTT+CGG | 0.345161 | 3.3:+59611434 | MS.gene63358:CDS |
| GATGCACAGGAACCTCTTCC+CGG | 0.347964 | 3.3:-59595969 | None:intergenic |
| TTTAGGTTGTCAGGGCATTA+AGG | 0.350842 | 3.3:+59599602 | MS.gene63358:intron |
| GGCATTAAGGTTTCTTCAAA+AGG | 0.354904 | 3.3:+59599615 | MS.gene63358:CDS |
| TTTGCTTTACAGGCAGCATC+TGG | 0.356148 | 3.3:+59599384 | MS.gene63358:intron |
| ATTCGCCTTAGCGGCAGCGC+TGG | 0.359435 | 3.3:-59595607 | None:intergenic |
| ATCATATGATCCATCTGCTT+TGG | 0.359942 | 3.3:+59608263 | MS.gene63358:CDS |
| AATCGATCCTCCACCGTCTC+AGG | 0.368068 | 3.3:+59595644 | MS.gene63358:CDS |
| GGAACCACTTGACCCTCCTT+TGG | 0.368385 | 3.3:-59599409 | None:intergenic |
| TCACAGTCCTTTATTGGAAC+TGG | 0.377035 | 3.3:+59607776 | MS.gene63358:CDS |
| CGATGCCAAATTCCAGTGTC+TGG | 0.379071 | 3.3:-59614935 | None:intergenic |
| AATCCAACACAAGCAGCTTC+AGG | 0.382037 | 3.3:-59597346 | None:intergenic |
| CTTTGACTTGTTTAGGTGCC+AGG | 0.386157 | 3.3:+59601118 | MS.gene63358:intron |
| TGCTTCAATGTTAGGACTTG+TGG | 0.386301 | 3.3:-59614872 | None:intergenic |
| CATTACTCAATTCCTGAAAC+TGG | 0.386382 | 3.3:+59602431 | MS.gene63358:CDS |
| GGGACCTATTGGTGCTTCCC+TGG | 0.387488 | 3.3:+59608284 | MS.gene63358:CDS |
| TTTACGTGTTAAAGGTTGTT+TGG | 0.389326 | 3.3:+59612761 | MS.gene63358:CDS |
| GTTTCGGACTAAGTGATGTT+TGG | 0.389512 | 3.3:+59611450 | MS.gene63358:CDS |
| GAAGTTATTGAGCTTGATAC+TGG | 0.392056 | 3.3:+59609038 | MS.gene63358:CDS |
| TCATATGATCCATCTGCTTT+GGG | 0.392240 | 3.3:+59608264 | MS.gene63358:CDS |
| GGATTAGAAATCAGAGTTGT+TGG | 0.394243 | 3.3:+59597364 | MS.gene63358:CDS |
| TTCATCAATGGGAGGTTTAA+CGG | 0.395685 | 3.3:+59595569 | None:intergenic |
| GTAAAACCTGTCGTCTTATT+GGG | 0.397956 | 3.3:-59612743 | None:intergenic |
| CGTGTTAAAGGTTGTTTGGC+TGG | 0.399193 | 3.3:+59612765 | MS.gene63358:CDS |
| ATGAGGGTCATGGTGTATTT+GGG | 0.399764 | 3.3:+59613196 | MS.gene63358:CDS |
| TCATAATTAGTTTCCAAGCT+AGG | 0.404713 | 3.3:-59606935 | None:intergenic |
| TAGAGCATGGAGAGTTTGTT+CGG | 0.406094 | 3.3:+59614822 | MS.gene63358:CDS |
| CATTCTGCAGTACGTGCTCT+TGG | 0.406112 | 3.3:+59613162 | MS.gene63358:CDS |
| GGGAGTCCTGCACATAGATA+TGG | 0.406421 | 3.3:+59614274 | MS.gene63358:CDS |
| CATACAAGATGATGGGTTAA+TGG | 0.406783 | 3.3:+59607102 | MS.gene63358:CDS |
| ACAATTCACAGGTGGAGTTA+TGG | 0.410566 | 3.3:+59614253 | MS.gene63358:intron |
| ACTTCTAAAATTCTCATAAG+TGG | 0.411543 | 3.3:-59611392 | None:intergenic |
| AGCCTATGAACATTTGGAAC+CGG | 0.412655 | 3.3:+59601147 | MS.gene63358:CDS |
| AGGAAGGGTCCGAGCTTTAT+GGG | 0.412772 | 3.3:+59609182 | MS.gene63358:CDS |
| TATGACACCAGTTCCAATAA+AGG | 0.414282 | 3.3:-59607783 | None:intergenic |
| AATTATTTCCTGGAAGTTAG+TGG | 0.416354 | 3.3:+59604915 | MS.gene63358:CDS |
| GGGGAGGTGCATACCATTCA+TGG | 0.419296 | 3.3:-59606721 | None:intergenic |
| TTACTGCTCCACTAACTTCC+AGG | 0.422846 | 3.3:-59604923 | None:intergenic |
| GCGTTTATCAACGACGAAAC+CGG | 0.423229 | 3.3:-59595790 | None:intergenic |
| CTTGTTTATGTGGCAGAACC+AGG | 0.424206 | 3.3:+59605416 | MS.gene63358:CDS |
| TATTGAATTGCAAATTGAAA+GGG | 0.426017 | 3.3:+59602485 | MS.gene63358:CDS |
| AAGATCATATCCGGTGCAAA+TGG | 0.426047 | 3.3:+59611332 | MS.gene63358:CDS |
| ATTTGCACTACTGGCCTACA+TGG | 0.430204 | 3.3:+59614897 | MS.gene63358:CDS |
| AATACCCCGGGGTACACTTC+AGG | 0.430346 | 3.3:+59599675 | MS.gene63358:CDS |
| ATACCGTACATCTTATAGAT+TGG | 0.430860 | 3.3:-59597999 | None:intergenic |
| GAGCGTGTAACTCAACATAT+TGG | 0.430891 | 3.3:+59606968 | MS.gene63358:CDS |
| AAGCTCAATAACTTCCATAT+TGG | 0.431622 | 3.3:-59609030 | None:intergenic |
| GTACTATTGCTCGTTTGGAT+AGG | 0.434020 | 3.3:+59597970 | MS.gene63358:CDS |
| GTGTCTTAAGAAAGTAGTTC+CGG | 0.434301 | 3.3:+59595698 | MS.gene63358:CDS |
| TACTATTGCTCGTTTGGATA+GGG | 0.434366 | 3.3:+59597971 | MS.gene63358:CDS |
| TCTTGGATTTCTTCCTAATG+AGG | 0.437933 | 3.3:+59613179 | MS.gene63358:CDS |
| ACGTTACAGAGCGACCAATA+TGG | 0.438339 | 3.3:+59609016 | MS.gene63358:CDS |
| CAGTGGATAGTTGAAATTAA+CGG | 0.438439 | 3.3:+59614310 | MS.gene63358:CDS |
| TTCTTGTGGGAGCAGATGTA+AGG | 0.440737 | 3.3:+59613082 | MS.gene63358:CDS |
| CATAATTAGTTTCCAAGCTA+GGG | 0.441473 | 3.3:-59606934 | None:intergenic |
| CATTAACCCATCATCTTGTA+TGG | 0.444029 | 3.3:-59607101 | None:intergenic |
| AAAAGCGAGGACCGGTGATT+CGG | 0.445203 | 3.3:-59595734 | None:intergenic |
| GTGGTTCCCCAGTTATAGAT+TGG | 0.446310 | 3.3:+59599424 | MS.gene63358:CDS |
| GTAAACAAACAGAAAGTAGA+TGG | 0.446684 | 3.3:+59607004 | MS.gene63358:CDS |
| CCATTGTGGCCTTGTTTATG+TGG | 0.448382 | 3.3:+59605406 | MS.gene63358:CDS |
| ACGCTGTAACTGCCGATGAC+TGG | 0.450612 | 3.3:+59595670 | MS.gene63358:CDS |
| CAGTTATAGATTGGCAAGGT+AGG | 0.453988 | 3.3:+59599433 | MS.gene63358:CDS |
| CAGTTCTGTCTAAGCTATCA+AGG | 0.454165 | 3.3:+59605903 | MS.gene63358:CDS |
| AATACACCATGACCCTCATT+AGG | 0.455137 | 3.3:-59613192 | None:intergenic |
| ATCACCCAGCGCTGCCGCTA+AGG | 0.456618 | 3.3:+59595602 | MS.gene63358:CDS |
| CCCTAATGATTTACCTCTCC+AGG | 0.458087 | 3.3:-59607911 | None:intergenic |
| GCATTAAGGTTTCTTCAAAA+GGG | 0.458502 | 3.3:+59599616 | MS.gene63358:CDS |
| ACCTGGCTTATCGAATAAAT+CGG | 0.461047 | 3.3:-59611302 | None:intergenic |
| TGGTCCAGCCTATGAACATT+TGG | 0.461472 | 3.3:+59601141 | MS.gene63358:CDS |
| GCTCCTGAAGCTGCTTGTGT+TGG | 0.464187 | 3.3:+59597343 | MS.gene63358:CDS |
| AGCATTCCAGTTTCAGTGGC+AGG | 0.468331 | 3.3:-59601008 | None:intergenic |
| GGCTTCTACTTAAGCCCAAA+AGG | 0.469473 | 3.3:-59608920 | None:intergenic |
| TTATCCAACATTACTTTCAA+AGG | 0.469848 | 3.3:+59611421 | MS.gene63358:CDS |
| CTAACTTCCAGGAAATAATT+GGG | 0.471403 | 3.3:-59604912 | None:intergenic |
| GTAATTCTGCAGCACGAACC+AGG | 0.472998 | 3.3:-59608302 | None:intergenic |
| TGTTGGTGAAGGTAACCTTC+AGG | 0.475030 | 3.3:+59606895 | MS.gene63358:CDS |
| GCATTAAATTTAGAATCTGC+TGG | 0.475600 | 3.3:+59608987 | MS.gene63358:CDS |
| ACCCGGTTCCAAATGTTCAT+AGG | 0.476665 | 3.3:-59601149 | None:intergenic |
| AGGCAATCGGGCTGAAAAGC+AGG | 0.477121 | 3.3:-59606788 | None:intergenic |
| AACCTTCAGGAGTTGGTTGG+TGG | 0.477151 | 3.3:+59606908 | MS.gene63358:CDS |
| CGAGCCTTTGAAAGTAATGT+TGG | 0.479406 | 3.3:-59611425 | None:intergenic |
| CGAGAGGTTGAACTTCTTGT+GGG | 0.482248 | 3.3:+59613069 | MS.gene63358:CDS |
| TTAACAAAAGAATCTAGATC+TGG | 0.483168 | 3.3:-59614343 | None:intergenic |
| CTTGGATTTCTTCCTAATGA+GGG | 0.483187 | 3.3:+59613180 | MS.gene63358:CDS |
| ACTCAACATATTGGATCTGA+TGG | 0.484766 | 3.3:+59606977 | MS.gene63358:CDS |
| ATGGAGAGTTTGTTCGGGTA+AGG | 0.486252 | 3.3:+59614828 | MS.gene63358:CDS |
| CCAACCTCTTTCATATCAGC+AGG | 0.486445 | 3.3:+59604947 | MS.gene63358:CDS |
| TCTAAATTTAATGCAGAGCA+AGG | 0.487100 | 3.3:-59608978 | None:intergenic |
| ACTGATTCTAGCTGCGCTAC+CGG | 0.487479 | 3.3:+59595771 | MS.gene63358:CDS |
| AACATAGCTTCAGCAACAAC+AGG | 0.489164 | 3.3:-59595940 | None:intergenic |
| TTCGCCTTAGCGGCAGCGCT+GGG | 0.489643 | 3.3:-59595606 | None:intergenic |
| GCAGCTAGAATCAGTGGTTT+CGG | 0.491014 | 3.3:-59595763 | None:intergenic |
| AGTCATCCAATTTCATTGAC+TGG | 0.491233 | 3.3:+59606851 | MS.gene63358:CDS |
| AGAGCATGGAGAGTTTGTTC+GGG | 0.491394 | 3.3:+59614823 | MS.gene63358:CDS |
| ATATATCACACTCAAGATAT+GGG | 0.491795 | 3.3:+59607806 | MS.gene63358:CDS |
| AAACAAGGCCACAATGGAAT+CGG | 0.491806 | 3.3:-59605400 | None:intergenic |
| TATTAGATTTATCTAAAGCT+TGG | 0.492288 | 3.3:-59612881 | None:intergenic |
| TGAAGCAAGATTTGCACTAC+TGG | 0.492489 | 3.3:+59614888 | MS.gene63358:CDS |
| GGCAGTGGCCCTGAATGCTG+GGG | 0.495366 | 3.3:+59599455 | MS.gene63358:CDS |
| TTATTCGATAAGCCAGGTCC+TGG | 0.496137 | 3.3:+59611307 | MS.gene63358:CDS |
| GTACCCCGGGGTATTGAAAC+CGG | 0.497585 | 3.3:-59599667 | None:intergenic |
| TGAAGGTAACCTTCAGGAGT+TGG | 0.498262 | 3.3:+59606901 | MS.gene63358:CDS |
| TGTTCATAGGCTGGACCACC+TGG | 0.499559 | 3.3:-59601136 | None:intergenic |
| ATGAGAGCATAATTGTGAAC+AGG | 0.499728 | 3.3:-59608240 | None:intergenic |
| CGAACCAGGGAAGCACCAAT+AGG | 0.500693 | 3.3:-59608288 | None:intergenic |
| AGGTTGTTTGGCTGGATCAA+AGG | 0.500857 | 3.3:+59612773 | MS.gene63358:CDS |
| GGTTGGTGGTGTCCCTAGCT+TGG | 0.501124 | 3.3:+59606922 | MS.gene63358:CDS |
| TCATCTGGGGCTAAAGATGT+TGG | 0.503587 | 3.3:+59606818 | MS.gene63358:CDS |
| ATCCAGGTTTCCATCTTGGC+CGG | 0.505196 | 3.3:+59597948 | MS.gene63358:intron |
| TCAGGTGCCATACAAGATGA+TGG | 0.505743 | 3.3:+59607094 | MS.gene63358:CDS |
| TTCTCATAAATGGTGTCAAA+CGG | 0.507395 | 3.3:+59611363 | MS.gene63358:CDS |
| GGTTCCAAATGTTCATAGGC+TGG | 0.507803 | 3.3:-59601145 | None:intergenic |
| ATATTGAATTGCAAATTGAA+AGG | 0.508331 | 3.3:+59602484 | MS.gene63358:CDS |
| ACTGGCCTACATGGGAATTG+AGG | 0.509066 | 3.3:+59614906 | MS.gene63358:CDS |
| GATGGGTTAATGGACTTTCA+AGG | 0.510838 | 3.3:+59607112 | MS.gene63358:CDS |
| GTCCTGGACAAGATCATATC+CGG | 0.512663 | 3.3:+59611323 | MS.gene63358:CDS |
| CTGATGGCTGCAGTCGGTGT+TGG | 0.514792 | 3.3:+59606685 | MS.gene63358:intron |
| GTCGTCGTTCTCCGAATCAC+CGG | 0.515596 | 3.3:+59595723 | MS.gene63358:CDS |
| ATTTAATGCAGAGCAAGGAT+TGG | 0.515688 | 3.3:-59608973 | None:intergenic |
| ATGGGGAAGCTTCTCCACTC+AGG | 0.517556 | 3.3:+59609200 | MS.gene63358:CDS |
| ATTCGGAGAACGACGACGGC+CGG | 0.518305 | 3.3:-59595717 | None:intergenic |
| CCCAGTTGAAATTCCTGGAG+AGG | 0.522398 | 3.3:+59607898 | MS.gene63358:CDS |
| TGACTGGTGAACGTGCTTGT+GGG | 0.523894 | 3.3:+59606867 | MS.gene63358:CDS |
| TTATGTTGATAAATGGAAGC+CGG | 0.523991 | 3.3:+59599648 | MS.gene63358:CDS |
| TCCGATTTATTCGATAAGCC+AGG | 0.524575 | 3.3:+59611301 | MS.gene63358:CDS |
| AAGGGGTGCTCGAGTACCAT+TGG | 0.525746 | 3.3:+59605922 | MS.gene63358:CDS |
| GCACAAATCATTCGCCTTAG+CGG | 0.525751 | 3.3:-59595616 | None:intergenic |
| AATCGCCATGTCGTTAAGCC+TGG | 0.528226 | 3.3:+59595831 | MS.gene63358:CDS |
| TTTCTTCCTAATGAGGGTCA+TGG | 0.529621 | 3.3:+59613186 | MS.gene63358:CDS |
| ATCAAAGAGCTTAACGCTGT+TGG | 0.529844 | 3.3:+59602515 | MS.gene63358:CDS |
| CTAACCTGCTGATATGAAAG+AGG | 0.530400 | 3.3:-59604951 | None:intergenic |
| CCTCTCCAGGAATTTCAACT+GGG | 0.531113 | 3.3:-59607898 | None:intergenic |
| AGTCAATGAAATTGGATGAC+TGG | 0.531237 | 3.3:-59606849 | None:intergenic |
| TTGACACCATTTATGAGAAG+AGG | 0.531326 | 3.3:-59611359 | None:intergenic |
| TTGACTGGTGAACGTGCTTG+TGG | 0.531978 | 3.3:+59606866 | MS.gene63358:CDS |
| TGCTTCCACGACAAATGCAT+CGG | 0.532551 | 3.3:+59607141 | MS.gene63358:CDS |
| GCAGATGTAAGGGATGGAAA+TGG | 0.533832 | 3.3:+59613093 | MS.gene63358:CDS |
| GATGTACTTGTTCGTGTCAA+TGG | 0.534103 | 3.3:+59601172 | MS.gene63358:CDS |
| CACCGGATATGATCTTGTCC+AGG | 0.534575 | 3.3:-59611325 | None:intergenic |
| ACGAGAGGTTGAACTTCTTG+TGG | 0.534855 | 3.3:+59613068 | MS.gene63358:CDS |
| AGATCATATCCGGTGCAAAT+GGG | 0.537309 | 3.3:+59611333 | MS.gene63358:CDS |
| AGCAATAGTACCGGCCAAGA+TGG | 0.538114 | 3.3:-59597958 | None:intergenic |
| TCTCTGTATATTGGATGCAC+AGG | 0.538426 | 3.3:-59595982 | None:intergenic |
| AAAATTCTCATAAGTGGCAT+AGG | 0.540469 | 3.3:-59611386 | None:intergenic |
| TACAAACCATATCTATGTGC+AGG | 0.542867 | 3.3:-59614280 | None:intergenic |
| CCCCTGTTCCAGCTGAAAGT+AGG | 0.544596 | 3.3:+59611242 | MS.gene63358:intron |
| ATATGATCTTGTCCAGGACC+TGG | 0.545650 | 3.3:-59611319 | None:intergenic |
| GTCATCCCGAGTGTATATCT+GGG | 0.546481 | 3.3:-59606741 | None:intergenic |
| TACCTTGCCAATCTATAACT+GGG | 0.546891 | 3.3:-59599431 | None:intergenic |
| AAAGAATCTAGATCTGGAGT+TGG | 0.548892 | 3.3:-59614337 | None:intergenic |
| CCTGCTGATATGAAAGAGGT+TGG | 0.549015 | 3.3:-59604947 | None:intergenic |
| GCAGCATCTGGGACCAAAGG+AGG | 0.549997 | 3.3:+59599396 | MS.gene63358:CDS |
| GTAGATGGTTTATCAACTGA+TGG | 0.550939 | 3.3:+59607019 | MS.gene63358:CDS |
| CATCAGAAGTGAGACAGAGC+AGG | 0.552016 | 3.3:+59600865 | MS.gene63358:CDS |
| ATTGAATTGCAAATTGAAAG+GGG | 0.552414 | 3.3:+59602486 | MS.gene63358:CDS |
| AAGACACTTCCGCCAGTCAT+CGG | 0.553782 | 3.3:-59595682 | None:intergenic |
| TTTGATGAGACTCGTCGACT+TGG | 0.555109 | 3.3:+59600842 | MS.gene63358:CDS |
| CACCTCCCCAGATATACACT+CGG | 0.555557 | 3.3:+59606735 | MS.gene63358:CDS |
| CCAATAGGTCCCAAAGCAGA+TGG | 0.560650 | 3.3:-59608273 | None:intergenic |
| GACTGGTGAACGTGCTTGTG+GGG | 0.561110 | 3.3:+59606868 | MS.gene63358:CDS |
| TATTGGTCGCTCTGTAACGT+GGG | 0.561763 | 3.3:-59609013 | None:intergenic |
| ATGAACACAATCAAGCATAC+AGG | 0.562042 | 3.3:-59607753 | None:intergenic |
| GCTTCCACGACAAATGCATC+GGG | 0.562254 | 3.3:+59607142 | MS.gene63358:CDS |
| TTGAGGTTTAATCCAGACAC+TGG | 0.562805 | 3.3:+59614923 | MS.gene63358:CDS |
| AGGCTTAACGACATGGCGAT+TGG | 0.566002 | 3.3:-59595829 | None:intergenic |
| GAAGCCGGTTTCAATACCCC+GGG | 0.567490 | 3.3:+59599663 | MS.gene63358:CDS |
| CACGACAAATGCATCGGGCA+CGG | 0.568939 | 3.3:+59607147 | MS.gene63358:CDS |
| ATATCAAGGAAGCATGTGAC+TGG | 0.569555 | 3.3:-59612846 | None:intergenic |
| AGTTATAGATTGGCAAGGTA+GGG | 0.572212 | 3.3:+59599434 | MS.gene63358:CDS |
| ATATTGGTCGCTCTGTAACG+TGG | 0.572451 | 3.3:-59609014 | None:intergenic |
| AAAAGATAGCAACACGTCAG+AGG | 0.573245 | 3.3:-59607867 | None:intergenic |
| CGTGCCCGATGCATTTGTCG+TGG | 0.574041 | 3.3:-59607146 | None:intergenic |
| CCACATAAACAAGGCCACAA+TGG | 0.574738 | 3.3:-59605406 | None:intergenic |
| GCCTATGAACATTTGGAACC+GGG | 0.577581 | 3.3:+59601148 | MS.gene63358:CDS |
| TACACTCGGGATGACAGTAC+TGG | 0.577972 | 3.3:+59606749 | MS.gene63358:CDS |
| GAAAGACCCAATAAGACGAC+AGG | 0.578073 | 3.3:+59612737 | MS.gene63358:CDS |
| AGATATATGCTCTCTAAAGC+AGG | 0.578187 | 3.3:+59605812 | MS.gene63358:intron |
| AGAGTTGTTGGTAATGATAG+TGG | 0.579124 | 3.3:+59597376 | MS.gene63358:CDS |
| AGTTCTGTCTAAGCTATCAA+GGG | 0.579194 | 3.3:+59605904 | MS.gene63358:CDS |
| ATGGTTTGTATGCTCTTCAG+TGG | 0.581891 | 3.3:+59614293 | MS.gene63358:CDS |
| GAATCTCCTCGACATAATGC+CGG | 0.582869 | 3.3:-59608891 | None:intergenic |
| CATTAAGGTTTCTTCAAAAG+GGG | 0.583184 | 3.3:+59599617 | MS.gene63358:CDS |
| CGCGGATCGTCATCGAAGGA+AGG | 0.583499 | 3.3:+59605958 | MS.gene63358:CDS |
| TGTTGAGTTACACGCTCTGA+AGG | 0.583835 | 3.3:-59606962 | None:intergenic |
| CAGGTGCCATACAAGATGAT+GGG | 0.584747 | 3.3:+59607095 | MS.gene63358:CDS |
| ACAAACCTGAAGTGTACCCC+GGG | 0.586694 | 3.3:-59599680 | None:intergenic |
| TGCTAACAGATGAACAAGGA+AGG | 0.588657 | 3.3:+59609166 | MS.gene63358:CDS |
| AACAAGCATTCCAGTTTCAG+TGG | 0.589397 | 3.3:-59601012 | None:intergenic |
| TTAAACCTCAATTCCCATGT+AGG | 0.589505 | 3.3:-59614911 | None:intergenic |
| GGAAGCCGGTTTCAATACCC+CGG | 0.590041 | 3.3:+59599662 | MS.gene63358:CDS |
| TGCTATCAGGAACTAGAGCA+TGG | 0.590136 | 3.3:+59614809 | MS.gene63358:intron |
| AAGCAGGCTTAGCAATCCAC+AGG | 0.590366 | 3.3:-59606772 | None:intergenic |
| CAGGCAGCATCTGGGACCAA+AGG | 0.592331 | 3.3:+59599393 | MS.gene63358:intron |
| TCTCTAAAGCAGGAGTTCCT+CGG | 0.594341 | 3.3:+59605822 | MS.gene63358:CDS |
| GACAAACCTGAAGTGTACCC+CGG | 0.594509 | 3.3:-59599681 | None:intergenic |
| CATCAACTCATCAGTTCGTG+AGG | 0.597000 | 3.3:+59611273 | MS.gene63358:CDS |
| CACCACCAACCAACTCCTGA+AGG | 0.597532 | 3.3:-59606910 | None:intergenic |
| GGTAACCTTCAGGAGTTGGT+TGG | 0.598214 | 3.3:+59606905 | MS.gene63358:CDS |
| CAAGCAGCTTCAGGAGCAAG+AGG | 0.598505 | 3.3:-59597337 | None:intergenic |
| TCTTGTGGGAGCAGATGTAA+GGG | 0.599880 | 3.3:+59613083 | MS.gene63358:CDS |
| AAGAGGTGACCCATTTGCAC+CGG | 0.600747 | 3.3:-59611342 | None:intergenic |
| GTAAGGACAATTCACCTCGA+TGG | 0.601347 | 3.3:+59614845 | MS.gene63358:CDS |
| TGACAGTTGATCGCCATGAA+TGG | 0.603508 | 3.3:+59606708 | MS.gene63358:CDS |
| TGAAACTGGAATGCTTGTTG+TGG | 0.606004 | 3.3:+59601016 | MS.gene63358:CDS |
| CTGGTTCTGCCACATAAACA+AGG | 0.606175 | 3.3:-59605415 | None:intergenic |
| CTCAACATATTGGATCTGAT+GGG | 0.606316 | 3.3:+59606978 | MS.gene63358:CDS |
| TTGCTTTACAGGCAGCATCT+GGG | 0.607141 | 3.3:+59599385 | MS.gene63358:intron |
| AGAAGCTTCCCCATAAAGCT+CGG | 0.607495 | 3.3:-59609191 | None:intergenic |
| ATCCAAACGAGCAATAGTAC+CGG | 0.610178 | 3.3:-59597967 | None:intergenic |
| TAACAACATTCATCAATGGG+AGG | 0.610986 | 3.3:+59595561 | None:intergenic |
| AGCAAGCTCACTCGATCCAG+AGG | 0.615151 | 3.3:-59599510 | None:intergenic |
| GGACTTGTGGCTTTCCATCG+AGG | 0.617641 | 3.3:-59614859 | None:intergenic |
| ACTCACCAGGCTTAACGACA+TGG | 0.620725 | 3.3:-59595836 | None:intergenic |
| GCGTAGCTTATATACTCCAA+TGG | 0.620735 | 3.3:-59605938 | None:intergenic |
| AGAAACTTCCGATTCCATTG+TGG | 0.620858 | 3.3:+59605392 | MS.gene63358:CDS |
| GGGACCAAAGGAGGGTCAAG+TGG | 0.621062 | 3.3:+59599405 | MS.gene63358:CDS |
| TAATTCTGCAGCACGAACCA+GGG | 0.622448 | 3.3:-59608301 | None:intergenic |
| GCAGTGGCCCTGAATGCTGG+GGG | 0.623590 | 3.3:+59599456 | MS.gene63358:CDS |
| GGGCAAAATATTAAGCGCAT+CGG | 0.624481 | 3.3:+59599476 | MS.gene63358:CDS |
| ATTTCCTAGAAGTTGCTCGA+AGG | 0.624658 | 3.3:-59608941 | None:intergenic |
| TCCCGAGTGTATATCTGGGG+AGG | 0.625073 | 3.3:-59606737 | None:intergenic |
| CAATGGGAGGTTTAACGGAG+AGG | 0.625682 | 3.3:+59595574 | None:intergenic |
| CAGCATCTGGGACCAAAGGA+GGG | 0.625718 | 3.3:+59599397 | MS.gene63358:CDS |
| TTGTTTATGTGGCAGAACCA+GGG | 0.625939 | 3.3:+59605417 | MS.gene63358:CDS |
| GAGCATAATTGTGAACAGGA+TGG | 0.626249 | 3.3:-59608236 | None:intergenic |
| TGACTTGTTTAGGTGCCAGG+TGG | 0.629016 | 3.3:+59601121 | MS.gene63358:intron |
| TCCCCAGTTATAGATTGGCA+AGG | 0.631830 | 3.3:+59599429 | MS.gene63358:CDS |
| ACTCGTACAGTTAATTGGTG+TGG | 0.632548 | 3.3:+59613120 | MS.gene63358:CDS |
| GCTACGCGGATCGTCATCGA+AGG | 0.633634 | 3.3:+59605954 | MS.gene63358:CDS |
| GTGGGAGCAGATGTAAGGGA+TGG | 0.634758 | 3.3:+59613087 | MS.gene63358:CDS |
| GTCATGGTGTATTTGGGACA+AGG | 0.634897 | 3.3:+59613202 | MS.gene63358:CDS |
| CAACTCATCAGTTCGTGAGG+GGG | 0.636136 | 3.3:+59611276 | MS.gene63358:CDS |
| ATCAACTCATCAGTTCGTGA+GGG | 0.639732 | 3.3:+59611274 | MS.gene63358:CDS |
| AGATTGGCAAGGTAGGGCAG+TGG | 0.639736 | 3.3:+59599440 | MS.gene63358:CDS |
| CTGTAACTGCCGATGACTGG+CGG | 0.642760 | 3.3:+59595673 | MS.gene63358:CDS |
| ACCTTGCCAATCTATAACTG+GGG | 0.644140 | 3.3:-59599430 | None:intergenic |
| AGAGCACGTACTGCAGAATG+TGG | 0.644495 | 3.3:-59613159 | None:intergenic |
| TTTGCACTACTGGCCTACAT+GGG | 0.645205 | 3.3:+59614898 | MS.gene63358:CDS |
| GGAAGGGTCCGAGCTTTATG+GGG | 0.645225 | 3.3:+59609183 | MS.gene63358:CDS |
| TGGTAATGATAGTGGTGAGA+AGG | 0.645560 | 3.3:+59597384 | MS.gene63358:CDS |
| TGCTTGTGTCTAGGGACGAG+AGG | 0.650818 | 3.3:+59613053 | MS.gene63358:intron |
| CCAGAGCCGGCATTATGTCG+AGG | 0.651448 | 3.3:+59608885 | MS.gene63358:intron |
| TCAACTCATCAGTTCGTGAG+GGG | 0.652329 | 3.3:+59611275 | MS.gene63358:CDS |
| ACACGAACAAGTACATCACC+CGG | 0.658346 | 3.3:-59601166 | None:intergenic |
| GCTAACAGATGAACAAGGAA+GGG | 0.658389 | 3.3:+59609167 | MS.gene63358:CDS |
| GGTGATTCGGAGAACGACGA+CGG | 0.658589 | 3.3:-59595721 | None:intergenic |
| ATAACTGGTAAGAACCTGAG+TGG | 0.658759 | 3.3:-59609214 | None:intergenic |
| TTCGTCGTTGATAAACGCCG+TGG | 0.658801 | 3.3:+59595795 | MS.gene63358:CDS |
| GGGATGACAGTACTGGCCTG+TGG | 0.659309 | 3.3:+59606756 | MS.gene63358:CDS |
| GGTAGCGCAGCTAGAATCAG+TGG | 0.663610 | 3.3:-59595769 | None:intergenic |
| ACACAATCAAGCATACAGGA+TGG | 0.673443 | 3.3:-59607749 | None:intergenic |
| ACCTCCCCAGATATACACTC+GGG | 0.676370 | 3.3:+59606736 | MS.gene63358:CDS |
| CGCTCATTCATACAGAACTG+TGG | 0.679587 | 3.3:-59614979 | None:intergenic |
| CAATCAAGCATACAGGATGG+AGG | 0.679860 | 3.3:-59607746 | None:intergenic |
| TCATCCCGAGTGTATATCTG+GGG | 0.682855 | 3.3:-59606740 | None:intergenic |
| GAATTGCAAATTGAAAGGGG+TGG | 0.697481 | 3.3:+59602489 | MS.gene63358:CDS |
| ACTGGTGAACGTGCTTGTGG+GGG | 0.704352 | 3.3:+59606869 | MS.gene63358:CDS |
| AATGCTTGTTGTGGAATCTG+CGG | 0.706997 | 3.3:+59601025 | MS.gene63358:CDS |
| GTTCTGTCTAAGCTATCAAG+GGG | 0.711059 | 3.3:+59605905 | MS.gene63358:CDS |
| GATTGGTAAGAATAATACCA+CGG | 0.738993 | 3.3:-59595812 | None:intergenic |
| TGAAGAAATATCTTGCCTCG+AGG | 0.765962 | 3.3:+59605871 | MS.gene63358:CDS |
| GGTGTGCTAACAGATGAACA+AGG | 0.786959 | 3.3:+59609162 | MS.gene63358:CDS |
| GAACTGATGTTAGATCCTCG+AGG | 0.788994 | 3.3:-59605886 | None:intergenic |
| AAGCCGGTTTCAATACCCCG+GGG | 0.792554 | 3.3:+59599664 | MS.gene63358:CDS |
| CAAACCTGAAGTGTACCCCG+GGG | 0.820259 | 3.3:-59599679 | None:intergenic |
| ATTGGAGTATATAAGCTACG+CGG | 0.822375 | 3.3:+59605940 | MS.gene63358:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 59595576 | 59614994 | 59595576 | ID=MS.gene63358 |
| chr3.3 | mRNA | 59595576 | 59614994 | 59595576 | ID=MS.gene63358.t1;Parent=MS.gene63358 |
| chr3.3 | exon | 59595576 | 59595852 | 59595576 | ID=MS.gene63358.t1.exon1;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59595576 | 59595852 | 59595576 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59595938 | 59596008 | 59595938 | ID=MS.gene63358.t1.exon2;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59595938 | 59596008 | 59595938 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59597271 | 59597405 | 59597271 | ID=MS.gene63358.t1.exon3;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59597271 | 59597405 | 59597271 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59597954 | 59598012 | 59597954 | ID=MS.gene63358.t1.exon4;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59597954 | 59598012 | 59597954 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59598113 | 59598149 | 59598113 | ID=MS.gene63358.t1.exon5;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59598113 | 59598149 | 59598113 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59599396 | 59599521 | 59599396 | ID=MS.gene63358.t1.exon6;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59599396 | 59599521 | 59599396 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59599607 | 59599696 | 59599607 | ID=MS.gene63358.t1.exon7;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59599607 | 59599696 | 59599607 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59600821 | 59600886 | 59600821 | ID=MS.gene63358.t1.exon8;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59600821 | 59600886 | 59600821 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59600990 | 59601046 | 59600990 | ID=MS.gene63358.t1.exon9;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59600990 | 59601046 | 59600990 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59601133 | 59601198 | 59601133 | ID=MS.gene63358.t1.exon10;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59601133 | 59601198 | 59601133 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59602429 | 59602536 | 59602429 | ID=MS.gene63358.t1.exon11;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59602429 | 59602536 | 59602429 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59604888 | 59604968 | 59604888 | ID=MS.gene63358.t1.exon12;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59604888 | 59604968 | 59604888 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59605389 | 59605438 | 59605389 | ID=MS.gene63358.t1.exon13;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59605389 | 59605438 | 59605389 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59605814 | 59605979 | 59605814 | ID=MS.gene63358.t1.exon14;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59605814 | 59605979 | 59605814 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59606698 | 59607204 | 59606698 | ID=MS.gene63358.t1.exon15;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59606698 | 59607204 | 59606698 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59607737 | 59607919 | 59607737 | ID=MS.gene63358.t1.exon16;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59607737 | 59607919 | 59607737 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59608225 | 59608330 | 59608225 | ID=MS.gene63358.t1.exon17;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59608225 | 59608330 | 59608225 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59608889 | 59609059 | 59608889 | ID=MS.gene63358.t1.exon18;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59608889 | 59609059 | 59608889 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59609142 | 59609221 | 59609142 | ID=MS.gene63358.t1.exon19;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59609142 | 59609221 | 59609142 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59611254 | 59611478 | 59611254 | ID=MS.gene63358.t1.exon20;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59611254 | 59611478 | 59611254 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59612726 | 59612941 | 59612726 | ID=MS.gene63358.t1.exon21;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59612726 | 59612941 | 59612726 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59613066 | 59613223 | 59613066 | ID=MS.gene63358.t1.exon22;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59613066 | 59613223 | 59613066 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59614264 | 59614375 | 59614264 | ID=MS.gene63358.t1.exon23;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59614264 | 59614375 | 59614264 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| chr3.3 | exon | 59614818 | 59614994 | 59614818 | ID=MS.gene63358.t1.exon24;Parent=MS.gene63358.t1 |
| chr3.3 | CDS | 59614818 | 59614994 | 59614818 | ID=cds.MS.gene63358.t1;Parent=MS.gene63358.t1 |
| Gene Sequence |
| Protein sequence |
