AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63481

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63481.t1 MTR_3g068035 96.805 313 5 3 1 308 1 313 0.0 594
MS.gene63481.t1 MTR_1g044155 52.077 313 133 3 1 305 55 358 2.19e-79 245
MS.gene63481.t1 MTR_1g079810 53.937 254 110 3 1 253 1 248 1.11e-69 219
MS.gene63481.t1 MTR_5g091630 52.232 224 84 2 76 299 47 247 2.82e-69 216
MS.gene63481.t1 MTR_1g073860 76.974 152 35 0 74 225 92 243 1.90e-68 216
MS.gene63481.t1 MTR_8g036060 57.727 220 76 1 27 229 20 239 3.08e-67 213
MS.gene63481.t1 MTR_5g080580 47.706 327 145 6 1 302 1 326 4.05e-66 210
MS.gene63481.t1 MTR_5g011520 61.780 191 61 1 52 230 64 254 4.07e-65 207
MS.gene63481.t1 MTR_7g080980 68.153 157 44 1 85 241 66 216 8.06e-62 197
MS.gene63481.t1 MTR_4g098450 66.480 179 58 1 53 229 51 229 2.24e-60 194
MS.gene63481.t1 MTR_3g100470 44.030 268 112 10 57 308 45 290 4.79e-58 188
MS.gene63481.t1 MTR_5g066020 54.362 149 63 3 85 228 60 208 5.63e-44 151
MS.gene63481.t1 MTR_5g089600 37.423 163 91 2 72 223 73 235 8.69e-30 113
MS.gene63481.t1 MTR_5g010330 36.416 173 103 4 76 242 104 275 5.83e-24 99.8
MS.gene63481.t1 MTR_6g061670 43.966 116 62 1 103 215 125 240 7.02e-24 99.4
MS.gene63481.t1 MTR_6g061670 43.966 116 62 1 103 215 130 245 7.58e-24 99.4
MS.gene63481.t1 MTR_5g089700 36.646 161 92 3 68 219 67 226 3.43e-23 95.9
MS.gene63481.t1 MTR_1g073870 46.053 152 35 4 74 225 50 154 3.50e-23 95.5
MS.gene63481.t1 MTR_4g057487 33.929 168 99 4 68 225 11 176 4.77e-23 95.5
MS.gene63481.t1 MTR_1g061530 42.982 114 61 2 106 215 144 257 9.96e-23 97.1
MS.gene63481.t1 MTR_1g079780 42.857 112 63 1 106 216 112 223 2.36e-22 95.5
MS.gene63481.t1 MTR_1g032910 32.530 166 96 5 72 223 97 260 1.71e-21 92.8
MS.gene63481.t1 MTR_1g053685 32.515 163 101 2 72 225 81 243 1.73e-21 92.4
MS.gene63481.t1 MTR_4g057525 33.333 162 98 3 72 223 32 193 2.92e-21 90.9
MS.gene63481.t1 MTR_5g089090 34.177 158 88 2 76 222 28 180 3.86e-21 89.7
MS.gene63481.t1 MTR_7g077120 36.420 162 94 3 74 229 125 283 8.40e-20 88.6
MS.gene63481.t1 MTR_8g036160 36.943 157 83 3 106 258 145 289 9.18e-19 85.9
MS.gene63481.t1 MTR_8g098390 36.496 137 83 3 95 228 158 293 9.40e-19 85.9
MS.gene63481.t1 MTR_1g076710 43.966 116 61 2 103 215 134 248 1.07e-18 85.5
MS.gene63481.t1 MTR_7g116320 42.609 115 64 2 102 215 142 255 3.07e-18 84.3
MS.gene63481.t1 MTR_4g098460 37.500 144 81 4 76 215 57 195 8.92e-18 82.0
MS.gene63481.t1 MTR_7g116320 42.063 126 67 3 103 223 143 267 1.19e-17 81.6
MS.gene63481.t1 MTR_7g074935 43.363 113 61 1 106 215 130 242 2.31e-17 81.6
MS.gene63481.t1 MTR_8g031100 41.096 146 80 3 76 215 125 270 2.74e-17 81.6
MS.gene63481.t1 MTR_5g011680 34.559 136 75 3 106 234 145 273 7.90e-17 79.3
MS.gene63481.t1 MTR_4g098460 36.667 150 79 4 76 215 57 200 2.32e-16 78.2
MS.gene63481.t1 MTR_4g055813 37.903 124 75 2 106 227 22 145 3.98e-16 77.4
MS.gene63481.t1 MTR_8g078930 34.028 144 81 4 107 237 5 147 4.46e-16 74.7
MS.gene63481.t1 MTR_4g069110 36.634 101 64 0 70 170 28 128 5.98e-16 74.7
MS.gene63481.t1 MTR_5g011620 31.210 157 92 4 107 261 119 261 2.08e-15 77.0
MS.gene63481.t1 MTR_4g055803 39.655 116 64 2 106 215 128 243 2.87e-15 76.3
MS.gene63481.t1 MTR_4g055833 41.176 119 58 3 106 215 125 240 3.66e-15 75.5
MS.gene63481.t1 MTR_4g101990 42.982 114 61 1 106 215 147 260 1.24e-14 73.6
MS.gene63481.t1 MTR_5g011610 33.333 117 75 2 107 222 62 176 1.57e-14 73.2
MS.gene63481.t1 MTR_5g011610 36.559 93 59 0 102 194 211 303 9.53e-13 68.2
MS.gene63481.t1 MTR_6g060670 40.541 111 64 2 106 216 180 288 1.94e-14 72.8
MS.gene63481.t1 MTR_1g071850 33.113 151 73 3 84 234 38 160 3.29e-13 67.4
MS.gene63481.t1 MTR_1g038960 33.333 144 88 4 77 216 125 264 1.19e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63481.t1 AT3G55560 52.647 340 99 10 1 308 1 310 1.56e-80 247
MS.gene63481.t1 AT4G14465 62.162 222 67 3 76 296 67 272 7.90e-75 231
MS.gene63481.t1 AT1G76500 60.541 185 63 2 52 227 49 232 3.31e-69 218
MS.gene63481.t1 AT2G42940 49.778 225 86 3 75 299 52 249 1.29e-64 204
MS.gene63481.t1 AT2G45430 56.596 235 86 6 76 302 89 315 2.36e-64 206
MS.gene63481.t1 AT3G04570 53.279 244 88 5 1 225 1 237 1.25e-63 204
MS.gene63481.t1 AT3G60870 51.982 227 87 4 76 302 59 263 2.14e-63 201
MS.gene63481.t1 AT2G35270 65.584 154 53 0 76 229 78 231 1.18e-62 200
MS.gene63481.t1 AT4G22810 70.064 157 44 1 76 229 105 261 7.29e-59 191
MS.gene63481.t1 AT4G12050 69.677 155 46 1 76 229 118 272 2.06e-58 191
MS.gene63481.t1 AT4G35390 59.296 199 79 1 27 223 14 212 4.48e-56 184
MS.gene63481.t1 AT5G49700 57.764 161 61 2 76 229 56 216 1.24e-55 182
MS.gene63481.t1 AT4G17800 66.207 145 49 0 85 229 91 235 2.47e-55 181
MS.gene63481.t1 AT1G20900 58.730 189 69 1 46 225 56 244 2.97e-53 177
MS.gene63481.t1 AT1G14490 48.913 184 85 3 66 242 14 195 1.13e-42 147
MS.gene63481.t1 AT1G14490 50.289 173 77 3 77 242 6 176 2.74e-42 145
MS.gene63481.t1 AT2G33620 43.243 148 80 3 71 215 132 278 7.41e-25 102
MS.gene63481.t1 AT2G33620 43.243 148 80 3 71 215 132 278 7.41e-25 102
MS.gene63481.t1 AT2G33620 43.243 148 80 3 71 215 132 278 7.41e-25 102
MS.gene63481.t1 AT2G33620 43.243 148 80 3 71 215 132 278 7.41e-25 102
MS.gene63481.t1 AT4G12080 44.538 119 59 2 106 219 173 289 1.01e-24 102
MS.gene63481.t1 AT4G25320 45.133 113 59 1 106 215 169 281 1.60e-23 99.8
MS.gene63481.t1 AT4G17950 42.759 145 76 3 76 215 196 338 1.80e-23 100
MS.gene63481.t1 AT5G51590 44.348 115 57 2 106 215 181 293 3.49e-23 99.0
MS.gene63481.t1 AT5G62260 38.710 124 73 1 103 223 160 283 5.53e-23 96.7
MS.gene63481.t1 AT2G45850 41.096 146 80 3 76 215 132 277 6.35e-23 97.4
MS.gene63481.t1 AT2G45850 41.096 146 80 3 76 215 132 277 6.35e-23 97.4
MS.gene63481.t1 AT1G63470 37.671 146 84 3 76 215 147 291 1.74e-22 96.7
MS.gene63481.t1 AT3G04590 42.727 110 63 0 106 215 174 283 2.79e-22 95.1
MS.gene63481.t1 AT3G04590 42.727 110 63 0 106 215 174 283 3.88e-22 95.9
MS.gene63481.t1 AT5G62260 39.655 116 67 1 103 215 160 275 6.92e-22 95.1
MS.gene63481.t1 AT5G62260 39.655 116 67 1 103 215 160 275 6.92e-22 95.1
MS.gene63481.t1 AT2G45850 40.136 147 82 3 76 216 132 278 4.45e-21 91.3
MS.gene63481.t1 AT4G00200 43.103 116 63 1 106 218 126 241 9.01e-18 82.8
MS.gene63481.t1 AT4G00200 43.103 116 63 1 106 218 126 241 9.01e-18 82.8
MS.gene63481.t1 AT4G22770 43.333 120 65 1 106 222 153 272 1.60e-17 82.0
MS.gene63481.t1 AT4G22770 43.333 120 65 1 106 222 153 272 1.60e-17 82.0
MS.gene63481.t1 AT3G61310 34.132 167 104 2 76 236 134 300 1.53e-16 79.3
MS.gene63481.t1 AT5G46640 40.972 144 80 2 76 216 155 296 1.57e-16 79.7
MS.gene63481.t1 AT1G63480 32.051 156 94 4 66 215 125 274 1.90e-16 79.3
MS.gene63481.t1 AT1G63480 32.051 156 94 4 66 215 125 274 1.90e-16 79.3
MS.gene63481.t1 AT1G63480 32.051 156 94 4 66 215 119 268 2.18e-16 79.0
MS.gene63481.t1 AT1G63480 32.051 156 94 4 66 215 119 268 2.18e-16 79.0
MS.gene63481.t1 AT1G63480 32.051 156 94 4 66 215 119 268 2.18e-16 79.0
MS.gene63481.t1 AT1G63480 32.051 156 94 4 66 215 119 268 2.18e-16 79.0
MS.gene63481.t1 AT1G63480 32.051 156 94 4 66 215 119 268 2.18e-16 79.0
MS.gene63481.t1 AT2G36560 33.043 115 72 2 106 215 108 222 9.25e-11 62.8

Find 100 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGAGATACTTTCGCTTTC+GGG 0.112808 3.1:-56351740 MS.gene63481:CDS
GCAGGTCCTTTGGTTGCTTC+TGG 0.205503 3.1:-56351626 MS.gene63481:CDS
GTACCATTATGCAACAAATT+TGG 0.220914 3.1:+56351377 None:intergenic
CAGGAGCAGCCGGCTGTCTA+AGG 0.232707 3.1:+56351789 None:intergenic
CCTTCTCCGGCTGGAGCTAC+TGG 0.276244 3.1:-56351701 MS.gene63481:CDS
TTCTTCTCAAGGTTTGGAAT+TGG 0.278560 3.1:-56351453 MS.gene63481:CDS
GGTTATAACAATGGGTGGTT+TGG 0.288461 3.1:+56351955 None:intergenic
TTGCTGTTGCTGCTGTTTGT+TGG 0.296688 3.1:+56352091 None:intergenic
GAAGGAGGAGGACGAGGAGG+TGG 0.303163 3.1:+56351323 None:intergenic
GTTATAACAATGGGTGGTTT+GGG 0.305692 3.1:+56351956 None:intergenic
AAATGCCTCATGATGTGTTT+TGG 0.306421 3.1:-56351352 MS.gene63481:CDS
CCTCCAAATTTGTTGCATAA+TGG 0.306945 3.1:-56351380 MS.gene63481:CDS
TCTTCTCAAGGTTTGGAATT+GGG 0.309314 3.1:-56351452 MS.gene63481:CDS
GGTTGCTTCTGGACCTGTTA+TGG 0.315696 3.1:-56351615 MS.gene63481:CDS
TGTTTATTTAGCTGGTGGTC+AGG 0.322450 3.1:-56351672 MS.gene63481:CDS
GGTGGTGTTATTACTCTTCA+TGG 0.329783 3.1:-56351767 MS.gene63481:CDS
TAGCTCTTCTTCTCAAGGTT+TGG 0.337613 3.1:-56351459 MS.gene63481:CDS
ATTCGAGATACTTTCGCTTT+CGG 0.341376 3.1:-56351741 MS.gene63481:CDS
GTTGTCGGTGGTTCTGTTGC+AGG 0.346809 3.1:-56351644 MS.gene63481:CDS
CTACCTCCGCCTTCTCCGGC+TGG 0.357947 3.1:-56351710 MS.gene63481:CDS
ACATGGCTTCTTAGCGCGTT+GGG 0.359556 3.1:+56351923 None:intergenic
GAAAAGAGAGCAAGAGTTGA+TGG 0.369566 3.1:-56352137 MS.gene63481:CDS
GGATTAACTGTTTATTTAGC+TGG 0.378249 3.1:-56351680 MS.gene63481:CDS
GGTGGTCAGGGGCAGGTTGT+CGG 0.381793 3.1:-56351659 MS.gene63481:CDS
GTAGCAGCAATAACCATAAC+AGG 0.415453 3.1:+56351602 None:intergenic
GTGGGCTGGAAATGTGGCTA+TGG 0.419505 3.1:-56352206 MS.gene63481:CDS
GGAGGAGGACGAGGAGGTGG+AGG 0.420358 3.1:+56351326 None:intergenic
TTTAGCTGGTGGTCAGGGGC+AGG 0.423340 3.1:-56351666 MS.gene63481:CDS
CTTAGACAGCCGGCTGCTCC+TGG 0.434867 3.1:-56351788 MS.gene63481:CDS
CCACTGCTACCTGGCTCAGA+TGG 0.446968 3.1:+56352022 None:intergenic
CTCCGACCACCACTGCTACC+TGG 0.451310 3.1:+56352013 None:intergenic
CGCGCTAAGAAGCCATGTCT+TGG 0.453833 3.1:-56351918 MS.gene63481:CDS
ATGGCAAATCGTTGGTGGGC+TGG 0.456372 3.1:-56352220 MS.gene63481:CDS
GACATGGCTTCTTAGCGCGT+TGG 0.461821 3.1:+56351922 None:intergenic
AGCTAACTCTTTCAGAAAGA+AGG 0.464345 3.1:+56351305 None:intergenic
TGGTTCTGTTGCAGGTCCTT+TGG 0.469676 3.1:-56351636 MS.gene63481:CDS
GTTACTAGCTCTTCTTCTCA+AGG 0.469725 3.1:-56351464 MS.gene63481:CDS
GTTTATTTAGCTGGTGGTCA+GGG 0.471442 3.1:-56351671 MS.gene63481:CDS
CACTGCTACCTGGCTCAGAT+GGG 0.484019 3.1:+56352023 None:intergenic
GTGACGGCGGTTAGCAAAGT+TGG 0.484913 3.1:+56351859 None:intergenic
CAGAAAGAAGGAGGAGGACG+AGG 0.494395 3.1:+56351317 None:intergenic
GCATCATCAACAATTACAAC+AGG 0.495081 3.1:-56351426 MS.gene63481:CDS
TTTGGAGGCAAATTATACAT+AGG 0.498673 3.1:+56351395 None:intergenic
GAAATGCAAGTTCAACAATC+TGG 0.502411 3.1:-56351515 MS.gene63481:CDS
TTCAGATGTTCAAGTGATGA+TGG 0.505534 3.1:+56352157 None:intergenic
ACCACCTTGTTCATCTTCCA+AGG 0.510963 3.1:+56351550 None:intergenic
GATTCTTTGGTTATAACAAT+GGG 0.518538 3.1:+56351947 None:intergenic
GGATTCTTTGGTTATAACAA+TGG 0.520979 3.1:+56351946 None:intergenic
TACTTATGAGAGGTTGCCCT+TGG 0.521945 3.1:-56351567 MS.gene63481:CDS
TGGGCTGGAAATGTGGCTAT+GGG 0.522347 3.1:-56352205 MS.gene63481:CDS
CCGCCGTCACCGTGGTGTGT+CGG 0.526432 3.1:-56351846 MS.gene63481:CDS
GTAGCTCCAGCCGGAGAAGG+CGG 0.528688 3.1:+56351704 None:intergenic
CCAGTAGCTCCAGCCGGAGA+AGG 0.532784 3.1:+56351701 None:intergenic
CACTGCTGATTTCCAAGACA+TGG 0.534335 3.1:+56351906 None:intergenic
GAACAAAACCCATCTGAGCC+AGG 0.536402 3.1:-56352031 MS.gene63481:CDS
AGGCCACGTGGGAGACCACC+AGG 0.537133 3.1:-56351992 MS.gene63481:CDS
GCTCCAGCCGGAGAAGGCGG+AGG 0.543492 3.1:+56351707 None:intergenic
TTAACTGTTTATTTAGCTGG+TGG 0.548179 3.1:-56351677 MS.gene63481:CDS
AAACCGACACACCACGGTGA+CGG 0.553487 3.1:+56351843 None:intergenic
CCATTATGCAACAAATTTGG+AGG 0.558327 3.1:+56351380 None:intergenic
TAATGTAACCCTTAGACAGC+CGG 0.563997 3.1:-56351798 MS.gene63481:CDS
AGATTATGGCAAATCGTTGG+TGG 0.564432 3.1:-56352225 None:intergenic
GGTCAGGGGCAGGTTGTCGG+TGG 0.567357 3.1:-56351656 MS.gene63481:CDS
ATAACACCACCAGGAGCAGC+CGG 0.567921 3.1:+56351779 None:intergenic
TTTATTTAGCTGGTGGTCAG+GGG 0.570136 3.1:-56351670 MS.gene63481:CDS
AGGAGCAGCCGGCTGTCTAA+GGG 0.570747 3.1:+56351790 None:intergenic
CATGGCTTCTTAGCGCGTTG+GGG 0.572748 3.1:+56351924 None:intergenic
TCAAGTGATGATGGTGATGA+AGG 0.576683 3.1:+56352166 None:intergenic
AGAAGATTATGGCAAATCGT+TGG 0.586611 3.1:-56352228 None:intergenic
TTGCTAATGCTACTTATGAG+AGG 0.586891 3.1:-56351577 MS.gene63481:CDS
TCTTTGGTTATAACAATGGG+TGG 0.587176 3.1:+56351950 None:intergenic
CTCTTTCAGAAAGAAGGAGG+AGG 0.588398 3.1:+56351311 None:intergenic
AGCCAGGTAGCAGTGGTGGT+CGG 0.589179 3.1:-56352015 MS.gene63481:CDS
AGTGGTGGTCGGAGGCCACG+TGG 0.591936 3.1:-56352004 MS.gene63481:CDS
GCAAGTTCAACAATCTGGTG+TGG 0.593311 3.1:-56351510 MS.gene63481:CDS
ATGGCTTCTTAGCGCGTTGG+GGG 0.597936 3.1:+56351925 None:intergenic
GATTATGGCAAATCGTTGGT+GGG 0.599720 3.1:-56352224 None:intergenic
ACAAGGTGGTGATGATCAAG+AGG 0.601496 3.1:-56351537 MS.gene63481:CDS
TCTGAGCCAGGTAGCAGTGG+TGG 0.601683 3.1:-56352019 MS.gene63481:CDS
CCATCTGAGCCAGGTAGCAG+TGG 0.602057 3.1:-56352022 MS.gene63481:CDS
CCACCTTGTTCATCTTCCAA+GGG 0.606730 3.1:+56351551 None:intergenic
AGACAGCCGGCTGCTCCTGG+TGG 0.608408 3.1:-56351785 MS.gene63481:CDS
AAAGAAGGAGGAGGACGAGG+AGG 0.613275 3.1:+56351320 None:intergenic
CCGACACACCACGGTGACGG+CGG 0.613394 3.1:+56351846 None:intergenic
CAGGTAGCAGTGGTGGTCGG+AGG 0.616953 3.1:-56352012 MS.gene63481:CDS
GTTAATCCAGTAGCTCCAGC+CGG 0.618525 3.1:+56351695 None:intergenic
GTGGTGGTCGGAGGCCACGT+GGG 0.622577 3.1:-56352003 MS.gene63481:CDS
TCGTTGGTGGGCTGGAAATG+TGG 0.623949 3.1:-56352212 MS.gene63481:CDS
CAGCAGTGGTAGTGATGTGG+CGG 0.628263 3.1:-56351891 MS.gene63481:CDS
CACTCAAAACCGACACACCA+CGG 0.631096 3.1:+56351837 None:intergenic
TAACTCTTTCAGAAAGAAGG+AGG 0.635542 3.1:+56351308 None:intergenic
TTTGCTAACCGCCGTCACCG+TGG 0.638394 3.1:-56351854 MS.gene63481:CDS
GGACCCCAAAACACATCATG+AGG 0.638475 3.1:+56351347 None:intergenic
GATCCTGGTGGTCTCCCACG+TGG 0.640272 3.1:+56351989 None:intergenic
TTGCCCTTGGAAGATGAACA+AGG 0.667444 3.1:-56351554 MS.gene63481:CDS
TGAAGAGTAATAACACCACC+AGG 0.674054 3.1:+56351770 None:intergenic
CATGTCTTGGAAATCAGCAG+TGG 0.685401 3.1:-56351905 MS.gene63481:CDS
ACAGGTCCAGAAGCAACCAA+AGG 0.692630 3.1:+56351620 None:intergenic
AATCAGCAGTGGTAGTGATG+TGG 0.709338 3.1:-56351894 MS.gene63481:CDS
CCCTTGGAAGATGAACAAGG+TGG 0.848232 3.1:-56351551 MS.gene63481:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GATTCTTTGGTTATAACAAT+GGG + chr3.1:56351592-56351611 None:intergenic 25.0%
GGATTAACTGTTTATTTAGC+TGG - chr3.1:56351856-56351875 MS.gene63481:CDS 30.0%
GGATTCTTTGGTTATAACAA+TGG + chr3.1:56351593-56351612 None:intergenic 30.0%
GTACCATTATGCAACAAATT+TGG + chr3.1:56352162-56352181 None:intergenic 30.0%
TTAACTGTTTATTTAGCTGG+TGG - chr3.1:56351859-56351878 MS.gene63481:CDS 30.0%
TTTGGAGGCAAATTATACAT+AGG + chr3.1:56352144-56352163 None:intergenic 30.0%
ATTCGAGATACTTTCGCTTT+CGG - chr3.1:56351795-56351814 MS.gene63481:CDS 35.0%
CCATTATGCAACAAATTTGG+AGG + chr3.1:56352159-56352178 None:intergenic 35.0%
GAAATGCAAGTTCAACAATC+TGG - chr3.1:56352021-56352040 MS.gene63481:CDS 35.0%
GCATCATCAACAATTACAAC+AGG - chr3.1:56352110-56352129 MS.gene63481:CDS 35.0%
GTTATAACAATGGGTGGTTT+GGG + chr3.1:56351583-56351602 None:intergenic 35.0%
TCTTTGGTTATAACAATGGG+TGG + chr3.1:56351589-56351608 None:intergenic 35.0%
TTCAGATGTTCAAGTGATGA+TGG + chr3.1:56351382-56351401 None:intergenic 35.0%
! AAATGCCTCATGATGTGTTT+TGG - chr3.1:56352184-56352203 MS.gene63481:CDS 35.0%
! AATGCCTCATGATGTGTTTT+GGG - chr3.1:56352185-56352204 MS.gene63481:CDS 35.0%
! TCTTCTCAAGGTTTGGAATT+GGG - chr3.1:56352084-56352103 MS.gene63481:CDS 35.0%
! TTCTTCTCAAGGTTTGGAAT+TGG - chr3.1:56352083-56352102 MS.gene63481:CDS 35.0%
! TTGCTAATGCTACTTATGAG+AGG - chr3.1:56351959-56351978 MS.gene63481:CDS 35.0%
!! CCTCCAAATTTGTTGCATAA+TGG - chr3.1:56352156-56352175 MS.gene63481:CDS 35.0%
CAACAACAACAACAACGAAG+AGG - chr3.1:56351465-56351484 MS.gene63481:CDS 40.0%
GAAAAGAGAGCAAGAGTTGA+TGG - chr3.1:56351399-56351418 MS.gene63481:CDS 40.0%
GGTTATAACAATGGGTGGTT+TGG + chr3.1:56351584-56351603 None:intergenic 40.0%
GTAGCAGCAATAACCATAAC+AGG + chr3.1:56351937-56351956 None:intergenic 40.0%
GTTTATTTAGCTGGTGGTCA+GGG - chr3.1:56351865-56351884 MS.gene63481:CDS 40.0%
TAATGTAACCCTTAGACAGC+CGG - chr3.1:56351738-56351757 MS.gene63481:CDS 40.0%
TAGCTCTTCTTCTCAAGGTT+TGG - chr3.1:56352077-56352096 MS.gene63481:CDS 40.0%
TGAAGAGTAATAACACCACC+AGG + chr3.1:56351769-56351788 None:intergenic 40.0%
TGTTTATTTAGCTGGTGGTC+AGG - chr3.1:56351864-56351883 MS.gene63481:CDS 40.0%
TTCGAGATACTTTCGCTTTC+GGG - chr3.1:56351796-56351815 MS.gene63481:CDS 40.0%
TTTATTTAGCTGGTGGTCAG+GGG - chr3.1:56351866-56351885 MS.gene63481:CDS 40.0%
! ATGCCTCATGATGTGTTTTG+GGG - chr3.1:56352186-56352205 MS.gene63481:CDS 40.0%
! GTTACTAGCTCTTCTTCTCA+AGG - chr3.1:56352072-56352091 MS.gene63481:CDS 40.0%
!! GGTGGTGTTATTACTCTTCA+TGG - chr3.1:56351769-56351788 MS.gene63481:CDS 40.0%
!! TCAAGTGATGATGGTGATGA+AGG + chr3.1:56351373-56351392 None:intergenic 40.0%
!!! TTGGGTTTGTTTTTGGATCC+TGG + chr3.1:56351565-56351584 None:intergenic 40.0%
ACCACCTTGTTCATCTTCCA+AGG + chr3.1:56351989-56352008 None:intergenic 45.0%
CATGTCTTGGAAATCAGCAG+TGG - chr3.1:56351631-56351650 MS.gene63481:CDS 45.0%
CCACCTTGTTCATCTTCCAA+GGG + chr3.1:56351988-56352007 None:intergenic 45.0%
GCAAGTTCAACAATCTGGTG+TGG - chr3.1:56352026-56352045 MS.gene63481:CDS 45.0%
TACTTATGAGAGGTTGCCCT+TGG - chr3.1:56351969-56351988 MS.gene63481:CDS 45.0%
TTGCCCTTGGAAGATGAACA+AGG - chr3.1:56351982-56352001 MS.gene63481:CDS 45.0%
! AATCAGCAGTGGTAGTGATG+TGG - chr3.1:56351642-56351661 MS.gene63481:CDS 45.0%
! ACAAGGTGGTGATGATCAAG+AGG - chr3.1:56351999-56352018 MS.gene63481:CDS 45.0%
! TTGCTGTTGCTGCTGTTTGT+TGG + chr3.1:56351448-56351467 None:intergenic 45.0%
!! CACTGCTGATTTCCAAGACA+TGG + chr3.1:56351633-56351652 None:intergenic 45.0%
!! GGGTGGTTTGGGTTTGTTTT+TGG + chr3.1:56351572-56351591 None:intergenic 45.0%
!!! GGTTTGTTTTTGGATCCTGG+TGG + chr3.1:56351562-56351581 None:intergenic 45.0%
ACAGGTCCAGAAGCAACCAA+AGG + chr3.1:56351919-56351938 None:intergenic 50.0%
ACATGGCTTCTTAGCGCGTT+GGG + chr3.1:56351616-56351635 None:intergenic 50.0%
CACTCAAAACCGACACACCA+CGG + chr3.1:56351702-56351721 None:intergenic 50.0%
CCCTTGGAAGATGAACAAGG+TGG - chr3.1:56351985-56352004 MS.gene63481:CDS 50.0%
GAACAAAACCCATCTGAGCC+AGG - chr3.1:56351505-56351524 MS.gene63481:CDS 50.0%
GGACCCCAAAACACATCATG+AGG + chr3.1:56352192-56352211 None:intergenic 50.0%
GGTTGCTTCTGGACCTGTTA+TGG - chr3.1:56351921-56351940 MS.gene63481:CDS 50.0%
GTTAATCCAGTAGCTCCAGC+CGG + chr3.1:56351844-56351863 None:intergenic 50.0%
! TGGGCTGGAAATGTGGCTAT+GGG - chr3.1:56351331-56351350 MS.gene63481:CDS 50.0%
! TGGTTCTGTTGCAGGTCCTT+TGG - chr3.1:56351900-56351919 MS.gene63481:CDS 50.0%
! TTTTCTACCTCCGCCTTCTC+CGG - chr3.1:56351822-56351841 MS.gene63481:CDS 50.0%
!!! GTGGTGTGTCGGTTTTGAGT+GGG - chr3.1:56351701-56351720 MS.gene63481:CDS 50.0%
AAACCGACACACCACGGTGA+CGG + chr3.1:56351696-56351715 None:intergenic 55.0%
AAAGAAGGAGGAGGACGAGG+AGG + chr3.1:56352219-56352238 None:intergenic 55.0%
ATAACACCACCAGGAGCAGC+CGG + chr3.1:56351760-56351779 None:intergenic 55.0%
ATGGCTTCTTAGCGCGTTGG+GGG + chr3.1:56351614-56351633 None:intergenic 55.0%
CACTGCTACCTGGCTCAGAT+GGG + chr3.1:56351516-56351535 None:intergenic 55.0%
CAGAAAGAAGGAGGAGGACG+AGG + chr3.1:56352222-56352241 None:intergenic 55.0%
CATGGCTTCTTAGCGCGTTG+GGG + chr3.1:56351615-56351634 None:intergenic 55.0%
CGCGCTAAGAAGCCATGTCT+TGG - chr3.1:56351618-56351637 MS.gene63481:CDS 55.0%
GACATGGCTTCTTAGCGCGT+TGG + chr3.1:56351617-56351636 None:intergenic 55.0%
GCAGGTCCTTTGGTTGCTTC+TGG - chr3.1:56351910-56351929 MS.gene63481:CDS 55.0%
GTGACGGCGGTTAGCAAAGT+TGG + chr3.1:56351680-56351699 None:intergenic 55.0%
TAGCGCGTTGGGGGATTCTT+TGG + chr3.1:56351605-56351624 None:intergenic 55.0%
! ATGGCAAATCGTTGGTGGGC+TGG - chr3.1:56351316-56351335 MS.gene63481:CDS 55.0%
! CAGCAGTGGTAGTGATGTGG+CGG - chr3.1:56351645-56351664 MS.gene63481:CDS 55.0%
! GTTGTCGGTGGTTCTGTTGC+AGG - chr3.1:56351892-56351911 MS.gene63481:CDS 55.0%
!! GTGGGCTGGAAATGTGGCTA+TGG - chr3.1:56351330-56351349 MS.gene63481:CDS 55.0%
!! TCGTTGGTGGGCTGGAAATG+TGG - chr3.1:56351324-56351343 MS.gene63481:CDS 55.0%
!!! CGTGGTGTGTCGGTTTTGAG+TGG - chr3.1:56351700-56351719 MS.gene63481:CDS 55.0%
!!! GTGTCGGTTTTGAGTGGGAG+CGG - chr3.1:56351706-56351725 MS.gene63481:CDS 55.0%
AGCCAGGTAGCAGTGGTGGT+CGG - chr3.1:56351521-56351540 MS.gene63481:CDS 60.0%
AGGAGCAGCCGGCTGTCTAA+GGG + chr3.1:56351749-56351768 None:intergenic 60.0%
CCACTGCTACCTGGCTCAGA+TGG + chr3.1:56351517-56351536 None:intergenic 60.0%
CCATCTGAGCCAGGTAGCAG+TGG - chr3.1:56351514-56351533 MS.gene63481:CDS 60.0%
TCTGAGCCAGGTAGCAGTGG+TGG - chr3.1:56351517-56351536 MS.gene63481:CDS 60.0%
TTTAGCTGGTGGTCAGGGGC+AGG - chr3.1:56351870-56351889 MS.gene63481:CDS 60.0%
TTTGCTAACCGCCGTCACCG+TGG - chr3.1:56351682-56351701 MS.gene63481:CDS 60.0%
CAGGAGCAGCCGGCTGTCTA+AGG + chr3.1:56351750-56351769 None:intergenic 65.0%
CAGGTAGCAGTGGTGGTCGG+AGG - chr3.1:56351524-56351543 MS.gene63481:CDS 65.0%
CCAGTAGCTCCAGCCGGAGA+AGG + chr3.1:56351838-56351857 None:intergenic 65.0%
CCTTCTCCGGCTGGAGCTAC+TGG - chr3.1:56351835-56351854 MS.gene63481:CDS 65.0%
CTCCGACCACCACTGCTACC+TGG + chr3.1:56351526-56351545 None:intergenic 65.0%
CTTAGACAGCCGGCTGCTCC+TGG - chr3.1:56351748-56351767 MS.gene63481:CDS 65.0%
GAAGGAGGAGGACGAGGAGG+TGG + chr3.1:56352216-56352235 None:intergenic 65.0%
GATCCTGGTGGTCTCCCACG+TGG + chr3.1:56351550-56351569 None:intergenic 65.0%
GGTGGTCAGGGGCAGGTTGT+CGG - chr3.1:56351877-56351896 MS.gene63481:CDS 65.0%
GTAGCTCCAGCCGGAGAAGG+CGG + chr3.1:56351835-56351854 None:intergenic 65.0%
AGACAGCCGGCTGCTCCTGG+TGG - chr3.1:56351751-56351770 MS.gene63481:CDS 70.0%
AGGCCACGTGGGAGACCACC+AGG - chr3.1:56351544-56351563 MS.gene63481:CDS 70.0%
AGTGGTGGTCGGAGGCCACG+TGG - chr3.1:56351532-56351551 MS.gene63481:CDS 70.0%
CCGCCGTCACCGTGGTGTGT+CGG - chr3.1:56351690-56351709 MS.gene63481:CDS 70.0%
CTACCTCCGCCTTCTCCGGC+TGG - chr3.1:56351826-56351845 MS.gene63481:CDS 70.0%
GGAGGAGGACGAGGAGGTGG+AGG + chr3.1:56352213-56352232 None:intergenic 70.0%
GGTCAGGGGCAGGTTGTCGG+TGG - chr3.1:56351880-56351899 MS.gene63481:CDS 70.0%
GTGGTGGTCGGAGGCCACGT+GGG - chr3.1:56351533-56351552 MS.gene63481:CDS 70.0%
! CCGACACACCACGGTGACGG+CGG + chr3.1:56351693-56351712 None:intergenic 70.0%
GCTCCAGCCGGAGAAGGCGG+AGG + chr3.1:56351832-56351851 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 56351316 56352242 56351316 ID=MS.gene63481
chr3.1 mRNA 56351316 56352242 56351316 ID=MS.gene63481.t1;Parent=MS.gene63481
chr3.1 exon 56351316 56352242 56351316 ID=MS.gene63481.t1.exon1;Parent=MS.gene63481.t1
chr3.1 CDS 56351316 56352242 56351316 ID=cds.MS.gene63481.t1;Parent=MS.gene63481.t1
Gene Sequence

>MS.gene63481

ATGGCAAATCGTTGGTGGGCTGGAAATGTGGCTATGGGTCACAGAGAATCAATGCCTTCATCACCATCATCACTTGAACATCTGAAAAGAGAGCAAGAGTTGATGGAAAACAACAACGCGAACTCAACTCCAACAAACAGCAGCAACAGCAACAACAACAACAACGAAGAGGAAGACAACAACAACGAAGAACAAAACCCATCTGAGCCAGGTAGCAGTGGTGGTCGGAGGCCACGTGGGAGACCACCAGGATCCAAAAACAAACCCAAACCACCCATTGTTATAACCAAAGAATCCCCCAACGCGCTAAGAAGCCATGTCTTGGAAATCAGCAGTGGTAGTGATGTGGCGGAGAGTATTGCCAACTTTGCTAACCGCCGTCACCGTGGTGTGTCGGTTTTGAGTGGGAGCGGCATTGTAGCTAATGTAACCCTTAGACAGCCGGCTGCTCCTGGTGGTGTTATTACTCTTCATGGAAGATTCGAGATACTTTCGCTTTCGGGTGCTTTTCTACCTCCGCCTTCTCCGGCTGGAGCTACTGGATTAACTGTTTATTTAGCTGGTGGTCAGGGGCAGGTTGTCGGTGGTTCTGTTGCAGGTCCTTTGGTTGCTTCTGGACCTGTTATGGTTATTGCTGCTACTTTTGCTAATGCTACTTATGAGAGGTTGCCCTTGGAAGATGAACAAGGTGGTGATGATCAAGAGGAAATGCAAGTTCAACAATCTGGTGTGGATTCAGCAACTGTTGCAGCTGCAGTTACTAGCTCTTCTTCTCAAGGTTTGGAATTGGGTGAGCATCATCAACAATTACAACAGGTTTCAATGCCTATGTATAATTTGCCTCCAAATTTGTTGCATAATGGTACTCAAATGCCTCATGATGTGTTTTGGGGTCCTCCACCTCCTCGTCCTCCTCCTTCTTTCTGA
Protein sequence

>MS.gene63481.t1

MANRWWAGNVAMGHRESMPSSPSSLEHLKREQELMENNNANSTPTNSSNSNNNNNEEEDNNNEEQNPSEPGSSGGRRPRGRPPGSKNKPKPPIVITKESPNALRSHVLEISSGSDVAESIANFANRRHRGVSVLSGSGIVANVTLRQPAAPGGVITLHGRFEILSLSGAFLPPPSPAGATGLTVYLAGGQGQVVGGSVAGPLVASGPVMVIAATFANATYERLPLEDEQGGDDQEEMQVQQSGVDSATVAAAVTSSSSQGLELGEHHQQLQQVSMPMYNLPPNLLHNGTQMPHDVFWGPPPPRPPPSF