AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055159


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055159.t1 MTR_7g065160 95.726 117 5 0 1 117 1 117 7.73e-80 230
MS.gene055159.t1 MTR_7g065210 87.179 117 15 0 1 117 1 117 6.74e-73 213
MS.gene055159.t1 MTR_8g070040 45.455 121 61 3 1 117 1 120 3.87e-25 92.0
MS.gene055159.t1 MTR_8g070015 42.105 114 61 3 1 110 1 113 2.30e-19 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055159.t1 AT5G05960 61.261 111 36 2 7 117 13 116 1.06e-42 136
MS.gene055159.t1 AT3G53980 64.211 95 33 1 23 117 21 114 2.86e-42 135
MS.gene055159.t1 AT3G53980 64.211 95 33 1 23 117 21 114 2.86e-42 135
MS.gene055159.t1 AT2G37870 49.107 112 51 4 10 117 6 115 5.31e-26 94.0

Find 21 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCAAGGTTGCAACGTTTA+GGG 0.315042 7.4:-39467906 None:intergenic
TCAGCAAGGTTGCAACGTTT+AGG 0.352849 7.4:-39467907 None:intergenic
GGTATAGCAGTTCTTGTTGC+AGG 0.362578 7.4:+39467652 MS.gene055159:CDS
AGCTTAAAAGCTTCATTGTC+TGG 0.411064 7.4:-39467721 None:intergenic
TCAAACGTTGCTAAAATGTC+TGG 0.431901 7.4:+39467856 MS.gene055159:CDS
GGATAGTAACAGCAATTTGA+GGG 0.472152 7.4:-39467885 None:intergenic
TGTGCTCAGGTTAAGAAACT+TGG 0.492815 7.4:+39467799 MS.gene055159:CDS
GTGAAATTGTTGTGCTTCGT+TGG 0.500117 7.4:+39467631 MS.gene055159:CDS
GGGATAGTAACAGCAATTTG+AGG 0.508534 7.4:-39467886 None:intergenic
GTCACAAAATTGTTGTGCTC+AGG 0.514410 7.4:+39467786 MS.gene055159:CDS
CGTCCACACTTGTAACCGAC+AGG 0.520932 7.4:-39467934 None:intergenic
CGCCCTGTCGGTTACAAGTG+TGG 0.534054 7.4:+39467931 MS.gene055159:CDS
GTTGAAAGTGCTGGTGAATG+TGG 0.539242 7.4:+39467685 MS.gene055159:CDS
AAAGTGCTGGTGAATGTGGA+AGG 0.549386 7.4:+39467689 MS.gene055159:CDS
AACCTTGCTGATCGCCCTGT+CGG 0.551684 7.4:+39467919 MS.gene055159:CDS
ATTCATAGAGTTGAAAGTGC+TGG 0.554882 7.4:+39467676 MS.gene055159:CDS
ATAACAGCACAAAGACAACT+TGG 0.604458 7.4:-39467829 None:intergenic
AGTGCTGGTGAATGTGGAAG+GGG 0.630405 7.4:+39467691 MS.gene055159:CDS
AACCGACAGGGCGATCAGCA+AGG 0.631783 7.4:-39467921 None:intergenic
AAGTGCTGGTGAATGTGGAA+GGG 0.641454 7.4:+39467690 MS.gene055159:CDS
GTCCACACTTGTAACCGACA+GGG 0.709589 7.4:-39467933 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTATTTATCTTTTTTCT+TGG + chr7.4:39468206-39468225 MS.gene055159:intron 10.0%
!!! ACTTATTTTGACATTTTTTT+AGG - chr7.4:39468167-39468186 None:intergenic 15.0%
AGCTTAAAAGCTTCATTGTC+TGG - chr7.4:39467724-39467743 None:intergenic 35.0%
ATAACAGCACAAAGACAACT+TGG - chr7.4:39467832-39467851 None:intergenic 35.0%
GGATAGTAACAGCAATTTGA+GGG - chr7.4:39467888-39467907 None:intergenic 35.0%
TCAAACGTTGCTAAAATGTC+TGG + chr7.4:39467856-39467875 MS.gene055159:CDS 35.0%
! ATTCATAGAGTTGAAAGTGC+TGG + chr7.4:39467676-39467695 MS.gene055159:CDS 35.0%
GGGATAGTAACAGCAATTTG+AGG - chr7.4:39467889-39467908 None:intergenic 40.0%
GTCACAAAATTGTTGTGCTC+AGG + chr7.4:39467786-39467805 MS.gene055159:CDS 40.0%
GTGAAATTGTTGTGCTTCGT+TGG + chr7.4:39467631-39467650 MS.gene055159:CDS 40.0%
TGTGCTCAGGTTAAGAAACT+TGG + chr7.4:39467799-39467818 MS.gene055159:CDS 40.0%
CAGCAAGGTTGCAACGTTTA+GGG - chr7.4:39467909-39467928 None:intergenic 45.0%
GGTATAGCAGTTCTTGTTGC+AGG + chr7.4:39467652-39467671 MS.gene055159:CDS 45.0%
TCAGCAAGGTTGCAACGTTT+AGG - chr7.4:39467910-39467929 None:intergenic 45.0%
! TCTTTTGCTGCACTTGCACA+AGG - chr7.4:39467751-39467770 None:intergenic 45.0%
!! AAAGTGCTGGTGAATGTGGA+AGG + chr7.4:39467689-39467708 MS.gene055159:CDS 45.0%
!! AAGTGCTGGTGAATGTGGAA+GGG + chr7.4:39467690-39467709 MS.gene055159:CDS 45.0%
!! GTTGAAAGTGCTGGTGAATG+TGG + chr7.4:39467685-39467704 MS.gene055159:CDS 45.0%
GTCCACACTTGTAACCGACA+GGG - chr7.4:39467936-39467955 None:intergenic 50.0%
!! AGTGCTGGTGAATGTGGAAG+GGG + chr7.4:39467691-39467710 MS.gene055159:CDS 50.0%
AACCTTGCTGATCGCCCTGT+CGG + chr7.4:39467919-39467938 MS.gene055159:CDS 55.0%
CGTCCACACTTGTAACCGAC+AGG - chr7.4:39467937-39467956 None:intergenic 55.0%
AACCGACAGGGCGATCAGCA+AGG - chr7.4:39467924-39467943 None:intergenic 60.0%
! CGCCCTGTCGGTTACAAGTG+TGG + chr7.4:39467931-39467950 MS.gene055159:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 39467619 39468293 39467619 ID=MS.gene055159
chr7.4 mRNA 39467619 39468293 39467619 ID=MS.gene055159.t1;Parent=MS.gene055159
chr7.4 exon 39467619 39467955 39467619 ID=MS.gene055159.t1.exon1;Parent=MS.gene055159.t1
chr7.4 CDS 39467619 39467955 39467619 ID=cds.MS.gene055159.t1;Parent=MS.gene055159.t1
chr7.4 exon 39468277 39468293 39468277 ID=MS.gene055159.t1.exon2;Parent=MS.gene055159.t1
chr7.4 CDS 39468277 39468293 39468277 ID=cds.MS.gene055159.t1;Parent=MS.gene055159.t1
Gene Sequence

>MS.gene055159

ATGGAGGGTCTTGTGAAATTGTTGTGCTTCGTTGGTATAGCAGTTCTTGTTGCAGGAATTCATAGAGTTGAAAGTGCTGGTGAATGTGGAAGGGGTACTACACCAGACAATGAAGCTTTTAAGCTACTTCCTTGTGCAAGTGCAGCAAAAGATGAAAATGCTAGTGTGTCACAAAATTGTTGTGCTCAGGTTAAGAAACTTGGTCAGAATCCAAGTTGTCTTTGTGCTGTTATGCTTTCAAACGTTGCTAAAATGTCTGGTGCTAACCCTCAAATTGCTGTTACTATCCCTAAACGTTGCAACCTTGCTGATCGCCCTGTCGGTTACAAGTGTGGACGTAAGTTACTAACTACTCTTTCGTTTTTGTAAAAAAATTGTTCGTATTTATTTGTTGTTTTCAAAGCTCAATGTTAGAGAAATAAGTGAAATAAAACTATAAATAGTTAAGAGTATTATAGAAAATAGATAAATAATTCTATCAAAAGTAAAAAAAAAAAAAATGCTAAAATCTACTTTCTTTTTATAGAACTAAAAACATACTTATGCCTAAAAAAATGTCAAAATAAGTATGCTGCTAATATATGTTTTAATTATTTATCTTTTTTCTTGGTAGATCAATATGCTAACTAATTCTTTTCACAATCTCTTAATATTGCAGCTTACACTCTTCCTTGA

Protein sequence

>MS.gene055159.t1

MEGLVKLLCFVGIAVLVAGIHRVESAGECGRGTTPDNEAFKLLPCASAAKDENASVSQNCCAQVKKLGQNPSCLCAVMLSNVAKMSGANPQIAVTIPKRCNLADRPVGYKCGPYTLP