AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63546

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63546.t1 MTR_3g010190 97.881 472 10 0 1 472 41 512 0.0 981
MS.gene63546.t1 MTR_8g071000 65.451 576 180 10 1 570 34 596 0.0 720
MS.gene63546.t1 MTR_1g022480 46.479 568 280 11 1 559 50 602 3.05e-158 467
MS.gene63546.t1 MTR_1g022480 46.643 566 280 11 1 559 50 600 2.78e-157 464
MS.gene63546.t1 MTR_7g056587 43.836 584 279 8 1 574 39 583 9.16e-157 462
MS.gene63546.t1 MTR_7g056587 43.836 584 279 8 1 574 47 591 1.57e-156 462
MS.gene63546.t1 MTR_7g056587 43.966 580 276 8 1 570 47 587 9.82e-156 460
MS.gene63546.t1 MTR_7g056587 43.966 580 276 8 1 570 61 601 1.83e-155 460
MS.gene63546.t1 MTR_4g070810 42.979 584 299 11 3 572 47 610 1.58e-154 457
MS.gene63546.t1 MTR_1g022480 46.073 573 280 12 1 559 50 607 2.60e-154 457
MS.gene63546.t1 MTR_3g117100 42.710 583 310 9 1 572 48 617 9.67e-153 453
MS.gene63546.t1 MTR_1g022480 45.509 501 252 10 65 559 31 516 1.97e-134 403
MS.gene63546.t1 MTR_3g112150 43.470 536 261 9 28 559 59 556 7.47e-134 403
MS.gene63546.t1 MTR_5g021050 57.756 303 125 2 1 303 17 316 1.17e-128 386
MS.gene63546.t1 MTR_5g021050 57.756 303 125 2 1 303 17 316 1.58e-128 386
MS.gene63546.t1 MTR_7g056587 42.515 501 239 8 80 570 1 462 9.46e-124 374
MS.gene63546.t1 MTR_3g117090 39.929 561 287 12 1 559 38 550 3.04e-122 373
MS.gene63546.t1 MTR_4g070720 40.212 567 275 14 1 559 39 549 1.14e-121 371
MS.gene63546.t1 MTR_3g117090 40.290 551 279 12 1 549 38 540 5.46e-121 369
MS.gene63546.t1 MTR_8g076880 38.809 554 270 11 22 560 39 538 8.08e-120 366
MS.gene63546.t1 MTR_5g012460 49.462 279 140 1 26 304 79 356 8.26e-94 295
MS.gene63546.t1 MTR_4g070790 51.136 264 128 1 43 306 65 327 8.34e-94 293
MS.gene63546.t1 MTR_6g015645 36.093 471 241 12 99 559 9 429 3.70e-92 291
MS.gene63546.t1 MTR_4g070780 40.708 339 150 11 16 346 20 315 1.11e-67 226
MS.gene63546.t1 MTR_4g070730 39.601 351 155 10 22 364 29 330 1.25e-66 222
MS.gene63546.t1 MTR_6g034285 52.941 102 48 0 98 199 195 296 1.64e-27 115
MS.gene63546.t1 MTR_3g098530 28.800 250 156 8 27 275 36 264 1.55e-16 80.5
MS.gene63546.t1 MTR_3g060670 30.180 222 135 6 57 270 26 235 2.87e-15 77.8
MS.gene63546.t1 MTR_2g027140 28.910 211 141 5 62 270 38 241 5.62e-15 76.6
MS.gene63546.t1 MTR_8g096170 26.613 248 160 8 33 277 21 249 1.85e-12 68.2
MS.gene63546.t1 MTR_8g096180 26.693 251 156 8 33 277 18 246 3.83e-12 67.0
MS.gene63546.t1 MTR_8g096180 27.381 252 163 10 33 277 18 256 7.15e-12 66.6
MS.gene63546.t1 MTR_7g070480 27.602 221 133 9 63 273 101 304 7.72e-12 68.2
MS.gene63546.t1 MTR_4g013300 29.279 222 129 10 63 273 126 330 8.17e-12 68.2
MS.gene63546.t1 MTR_1g022185 27.016 248 152 10 32 273 15 239 6.00e-11 64.3
MS.gene63546.t1 MTR_8g095360 27.468 233 136 10 63 282 182 394 9.96e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63546.t1 AT1G55690 60.929 581 218 7 1 573 40 619 0.0 670
MS.gene63546.t1 AT1G55690 61.565 575 212 7 1 567 40 613 0.0 669
MS.gene63546.t1 AT1G55690 61.565 575 212 7 1 567 40 613 0.0 669
MS.gene63546.t1 AT1G55690 61.565 575 212 7 1 567 40 613 0.0 669
MS.gene63546.t1 AT1G55690 61.565 575 212 7 1 567 40 613 0.0 669
MS.gene63546.t1 AT5G56160 48.940 566 255 5 1 565 34 566 0.0 535
MS.gene63546.t1 AT5G56160 48.940 566 255 5 1 565 34 566 0.0 535
MS.gene63546.t1 AT5G56160 66.975 324 104 2 1 323 34 355 1.60e-161 471
MS.gene63546.t1 AT2G21520 42.953 596 267 10 1 559 52 611 2.38e-157 465
MS.gene63546.t1 AT2G21520 42.953 596 267 10 1 559 52 611 2.38e-157 465
MS.gene63546.t1 AT2G21520 42.953 596 267 10 1 559 48 607 4.15e-157 464
MS.gene63546.t1 AT4G39170 44.621 567 280 12 1 559 48 588 3.67e-154 456
MS.gene63546.t1 AT4G39170 44.268 567 284 11 1 559 48 590 6.64e-154 456
MS.gene63546.t1 AT3G24840 44.112 569 264 10 1 568 43 558 2.15e-149 443
MS.gene63546.t1 AT3G24840 44.112 569 264 10 1 568 43 558 2.15e-149 443
MS.gene63546.t1 AT3G24840 44.112 569 264 10 1 568 43 558 2.15e-149 443
MS.gene63546.t1 AT3G24840 44.112 569 264 10 1 568 43 558 2.15e-149 443
MS.gene63546.t1 AT1G19650 42.314 579 295 11 7 572 50 602 4.58e-145 433
MS.gene63546.t1 AT1G19650 54.017 361 162 4 7 366 50 407 1.40e-134 400
MS.gene63546.t1 AT1G75370 42.782 568 280 13 9 563 58 593 2.03e-133 403
MS.gene63546.t1 AT3G24840 60.261 307 120 2 1 307 43 347 3.72e-133 401
MS.gene63546.t1 AT3G24840 46.575 73 39 0 496 568 487 559 5.69e-12 68.9
MS.gene63546.t1 AT3G24840 60.261 307 120 2 1 307 43 347 3.72e-133 401
MS.gene63546.t1 AT3G24840 46.575 73 39 0 496 568 487 559 5.69e-12 68.9
MS.gene63546.t1 AT3G24840 60.261 307 120 2 1 307 43 347 3.72e-133 401
MS.gene63546.t1 AT3G24840 46.575 73 39 0 496 568 487 559 5.69e-12 68.9
MS.gene63546.t1 AT4G39180 56.211 322 138 2 1 322 37 355 1.96e-132 399
MS.gene63546.t1 AT4G39180 56.211 322 138 2 1 322 37 355 1.96e-132 399
MS.gene63546.t1 AT4G39180 56.211 322 138 2 1 322 37 355 1.96e-132 399
MS.gene63546.t1 AT1G75370 42.782 568 280 13 9 563 58 593 2.03e-132 402
MS.gene63546.t1 AT2G21540 51.087 368 168 3 1 359 36 400 4.79e-130 392
MS.gene63546.t1 AT2G21540 51.087 368 168 3 1 359 36 400 4.79e-130 392
MS.gene63546.t1 AT2G21540 51.087 368 168 3 1 359 36 400 4.79e-130 392
MS.gene63546.t1 AT2G21540 51.087 368 168 3 1 359 36 400 4.79e-130 392
MS.gene63546.t1 AT2G21540 57.419 310 129 2 1 310 36 342 2.14e-129 390
MS.gene63546.t1 AT2G21540 57.419 310 129 2 1 310 36 342 2.14e-129 390
MS.gene63546.t1 AT1G19650 41.556 527 270 10 59 572 29 530 4.90e-129 389
MS.gene63546.t1 AT2G18180 40.142 563 274 14 3 559 24 529 3.59e-128 387
MS.gene63546.t1 AT2G18180 39.785 558 277 12 3 556 24 526 3.66e-128 388
MS.gene63546.t1 AT4G34580 55.738 305 133 2 1 305 28 330 7.16e-126 382
MS.gene63546.t1 AT4G34580 55.738 305 133 2 1 305 28 330 7.16e-126 382
MS.gene63546.t1 AT4G36490 38.476 538 280 7 28 564 44 531 2.19e-125 380
MS.gene63546.t1 AT2G18180 39.882 509 245 13 62 564 10 463 2.31e-117 357
MS.gene63546.t1 AT2G18180 39.882 509 245 13 62 564 10 463 3.43e-117 357
MS.gene63546.t1 AT2G16380 53.420 307 141 2 1 307 28 332 6.20e-117 358
MS.gene63546.t1 AT2G16380 53.420 307 141 2 1 307 28 332 6.20e-117 358
MS.gene63546.t1 AT2G18180 39.308 491 237 13 80 564 1 436 2.05e-109 335
MS.gene63546.t1 AT2G16380 58.772 228 93 1 80 307 1 227 3.95e-99 309
MS.gene63546.t1 AT5G47510 50.769 260 127 1 37 296 26 284 4.29e-93 290
MS.gene63546.t1 AT5G47510 50.769 260 127 1 37 296 25 283 1.19e-92 290
MS.gene63546.t1 AT5G47510 50.769 260 127 1 37 296 26 284 1.32e-92 290
MS.gene63546.t1 AT5G47510 51.542 227 109 1 70 296 2 227 1.07e-82 262
MS.gene63546.t1 AT1G55840 29.528 254 159 7 37 281 6 248 8.37e-18 85.5
MS.gene63546.t1 AT1G55840 29.694 229 146 6 57 281 4 221 2.02e-17 83.6
MS.gene63546.t1 AT1G01630 30.400 250 141 9 31 273 22 245 7.13e-17 81.3
MS.gene63546.t1 AT5G47730 29.048 210 140 4 63 270 39 241 4.97e-16 80.1
MS.gene63546.t1 AT5G47730 29.048 210 140 4 63 270 39 241 4.97e-16 80.1
MS.gene63546.t1 AT1G72160 25.210 238 152 10 53 277 155 379 3.95e-11 66.2

Find 127 sgRNAs with CRISPR-Local

Find 276 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGTATAAGATTTGAATTT+TGG 0.180122 3.3:-5172034 MS.gene63546:CDS
CCTGTATCTTTGCAATAGTT+TGG 0.232046 3.3:+5173395 None:intergenic
CAATTTGCTTAGCATTATTA+AGG 0.247877 3.3:-5172135 MS.gene63546:CDS
TGCCTCGCCTCGGGCTTCCT+TGG 0.252176 3.3:+5171212 None:intergenic
AAAGGGGATCTTTGCCTTCT+AGG 0.258656 3.3:-5175224 MS.gene63546:CDS
CTGTATCTTTGCAATAGTTT+GGG 0.262582 3.3:+5173396 None:intergenic
GTCAAAATTGTTCTGTTCAT+TGG 0.274378 3.3:+5172878 None:intergenic
TCAATGCTGGTACTGGCTTT+AGG 0.289975 3.3:-5173456 MS.gene63546:CDS
GCTTGAAATTAATGATTTAC+TGG 0.298005 3.3:-5171646 MS.gene63546:CDS
ATCCCCTTTCGACAAGTCTT+TGG 0.312939 3.3:+5175238 None:intergenic
AACATTACCTTCATGATATC+AGG 0.313347 3.3:+5173066 None:intergenic
GTCATTGCAAACTAATGATA+TGG 0.327390 3.3:-5172398 MS.gene63546:CDS
GCCTCGCCTCGGGCTTCCTT+GGG 0.328711 3.3:+5171213 None:intergenic
GAAATCTCTATATAAATTAC+AGG 0.365965 3.3:-5173269 MS.gene63546:CDS
GGCGAGGCATTCAAGTCTAA+TGG 0.366040 3.3:-5171198 None:intergenic
GGAGAAGGAGCAAATGCTTA+TGG 0.367860 3.3:-5171838 MS.gene63546:CDS
CTTACGTGGCGAAAGGAGTA+TGG 0.370156 3.3:-5174922 MS.gene63546:CDS
AAACTTACTTCTTCACAAAC+TGG 0.377431 3.3:+5172611 None:intergenic
CGGTTTACATTGAGAGGCTT+GGG 0.379178 3.3:-5174123 MS.gene63546:CDS
ACATGAACCACCAAGAAAAT+CGG 0.380730 3.3:+5173143 None:intergenic
CACTTAAGAAAAGAGGAAAA+AGG 0.383154 3.3:-5175341 MS.gene63546:CDS
CTGGCTTTAGGAAGATGCTT+TGG 0.389604 3.3:-5173444 MS.gene63546:CDS
ACAAGTGCTGACATCCTTCA+TGG 0.393015 3.3:-5172078 MS.gene63546:CDS
TCCTTTAAAAGTCAGATATC+AGG 0.394555 3.3:-5171265 MS.gene63546:intron
CACCAAGAAAATCGGGCAAC+TGG 0.403587 3.3:+5173151 None:intergenic
CAAGTCTTTGGCGCAATTCA+AGG 0.405426 3.3:+5175250 None:intergenic
CTGGTGAAGGTGGATGTCTT+AGG 0.410160 3.3:-5173113 MS.gene63546:CDS
TGATAATCATCATGCCTAGA+AGG 0.422308 3.3:+5175210 None:intergenic
TTCTCCAAAGGCATGCCATC+AGG 0.423462 3.3:+5171855 None:intergenic
CCGGTTTACATTGAGAGGCT+TGG 0.426846 3.3:-5174124 MS.gene63546:CDS
AGCGACTCTTCAGCAGAATC+AGG 0.433420 3.3:-5172698 MS.gene63546:CDS
ACTCATTCACTTAAGAAAAG+AGG 0.446379 3.3:-5175348 MS.gene63546:CDS
CCAAACTATTGCAAAGATAC+AGG 0.446618 3.3:-5173395 MS.gene63546:intron
GCAAATGCTTATGGAATCCT+TGG 0.448250 3.3:-5171829 MS.gene63546:CDS
CTGTAGTAGCCATTCACATC+TGG 0.454523 3.3:+5172490 None:intergenic
AACAACAATACTGGATGTAC+AGG 0.455740 3.3:-5173963 MS.gene63546:CDS
CAAACCTGATGGCATGCCTT+TGG 0.456974 3.3:-5171859 MS.gene63546:CDS
GCAATGACTGCTCACGAGTA+AGG 0.461203 3.3:+5172413 None:intergenic
ACGCGTTCTGTGGAATTTGA+TGG 0.465779 3.3:+5171990 None:intergenic
TACATTGTCAATGCTGGTAC+TGG 0.471435 3.3:-5173463 MS.gene63546:CDS
AGACTTGAATGCCTCGCCTC+GGG 0.474212 3.3:+5171203 None:intergenic
CCAAGCCTCTCAATGTAAAC+CGG 0.476962 3.3:+5174124 None:intergenic
AGGTTACCATGGAGTTGATA+AGG 0.479015 3.3:-5174155 MS.gene63546:CDS
TAGTATTATTACCTCAAGAA+TGG 0.481409 3.3:+5174891 None:intergenic
CAACTCCATGGTAACCTTGA+GGG 0.488317 3.3:+5174161 None:intergenic
ATATGGAGAATATTGCTTGT+AGG 0.489787 3.3:-5172381 MS.gene63546:CDS
GCGCCAAAGACTTGTCGAAA+GGG 0.494515 3.3:-5175241 MS.gene63546:CDS
ATAACTACATCTCCTTTGTC+TGG 0.496146 3.3:+5172646 None:intergenic
TGAGAAAACAATTGAGATGT+GGG 0.499593 3.3:-5174953 MS.gene63546:CDS
AATCTGTCTCTTCGCTGCAA+TGG 0.499997 3.3:+5173994 None:intergenic
AGCAAATCTCTTGGCTTCCA+TGG 0.500376 3.3:-5173615 MS.gene63546:CDS
CTCTGAAGACGTTGTTCACA+AGG 0.509978 3.3:+5171912 None:intergenic
CTACATCTCCTTTGTCTGGT+AGG 0.510607 3.3:+5172650 None:intergenic
TAGACTTGAATGCCTCGCCT+CGG 0.511560 3.3:+5171202 None:intergenic
CATGAACCACCAAGAAAATC+GGG 0.518291 3.3:+5173144 None:intergenic
TCTATGAACTTCTGTGCAGC+AGG 0.519372 3.3:+5173421 None:intergenic
ACGTCTTCAGAGGCTAGAAA+AGG 0.520954 3.3:-5171901 MS.gene63546:CDS
GGTTCCACCAGTGGCTATTG+AGG 0.523124 3.3:-5175304 MS.gene63546:CDS
CTCTCTCAAACTCTTGTACA+TGG 0.528841 3.3:+5174044 None:intergenic
TCGCACAGTCGAGCGTTGAT+CGG 0.530295 3.3:-5171237 MS.gene63546:CDS
AACGGACTTTATTCTATCCA+AGG 0.531191 3.3:+5171812 None:intergenic
ATGAAGGATGTCAGCACTTG+TGG 0.531732 3.3:+5172080 None:intergenic
CAAATGTACATTGTCAATGC+TGG 0.538652 3.3:-5173469 MS.gene63546:CDS
AATACTGGATGTACAGGGCT+TGG 0.540642 3.3:-5173957 MS.gene63546:intron
GTTAATAATGACGCGTTCTG+TGG 0.541907 3.3:+5171980 None:intergenic
TTGAGAAAACAATTGAGATG+TGG 0.543568 3.3:-5174954 MS.gene63546:CDS
TGATGGCATGCCTTTGGAGA+AGG 0.546033 3.3:-5171853 MS.gene63546:CDS
CGCGTTCTGTGGAATTTGAT+GGG 0.546574 3.3:+5171991 None:intergenic
TTCGCTGCAATGGAACATGC+TGG 0.547083 3.3:+5174004 None:intergenic
TTAGGTCTAACAAGGGTCCA+TGG 0.550541 3.3:-5173095 MS.gene63546:CDS
ATACCCTCTTTGTCTTCTCA+AGG 0.552784 3.3:+5171781 None:intergenic
GCGTACTGCAGCAAATCTCT+TGG 0.558703 3.3:-5173624 MS.gene63546:CDS
GATCAACTTACTCGACACTT+CGG 0.560086 3.3:+5171609 None:intergenic
CGCAGCGAGTCTTTCCATGA+AGG 0.560463 3.3:+5172064 None:intergenic
TTTCAAATATCGATCTATTG+TGG 0.561616 3.3:+5174069 None:intergenic
CTAAGACATCCACCTTCACC+AGG 0.567033 3.3:+5173114 None:intergenic
TGTATCTTTGCAATAGTTTG+GGG 0.567207 3.3:+5173397 None:intergenic
GAACTCAATAACAAACCTGA+TGG 0.570432 3.3:-5171870 MS.gene63546:CDS
GTAGGCCGGTTTACATTGAG+AGG 0.571661 3.3:-5174129 MS.gene63546:CDS
TTGATTCAATAGCTGCGGAA+TGG 0.576539 3.3:+5171958 None:intergenic
GGTGGTTCATGTAAATGTCC+TGG 0.577495 3.3:-5173132 MS.gene63546:CDS
ACCCAAGGAAGCCCGAGGCG+AGG 0.578073 3.3:-5171214 MS.gene63546:CDS
AGCATTTGCTCCTTCTCCAA+AGG 0.579422 3.3:+5171843 None:intergenic
CTTGTGAACAACGTCTTCAG+AGG 0.580555 3.3:-5171911 MS.gene63546:CDS
CTAACATCCTCAATAGCCAC+TGG 0.581497 3.3:+5175297 None:intergenic
TGCGCCAAAGACTTGTCGAA+AGG 0.582230 3.3:-5175242 MS.gene63546:CDS
AGTTGATAAGGAAGGTAGGC+CGG 0.589330 3.3:-5174143 MS.gene63546:CDS
TACCTTCCTTATCAACTCCA+TGG 0.591048 3.3:+5174149 None:intergenic
GTATTGCAGTATTACCCTCA+AGG 0.591530 3.3:-5174175 MS.gene63546:CDS
ATGATATCAGGATCATTCCA+TGG 0.591683 3.3:+5173078 None:intergenic
CTTCTCAAGGTCAAACTCAA+CGG 0.592403 3.3:+5171794 None:intergenic
GTTCTGTTCATTGGACGCCC+TGG 0.594168 3.3:+5172887 None:intergenic
GTGATGTGCATCAAGCGACT+TGG 0.595132 3.3:+5174091 None:intergenic
CGCCAAAGACTTGTCGAAAG+GGG 0.597902 3.3:-5175240 MS.gene63546:CDS
TGGATGTCTTAGGTCTAACA+AGG 0.599523 3.3:-5173103 MS.gene63546:CDS
CTTCAGTGAGTGTAACTGAA+AGG 0.601782 3.3:+5172854 None:intergenic
TGACTATAGGGTTCCACCAG+TGG 0.601959 3.3:-5175313 MS.gene63546:CDS
GTCAGGGTACCAGATGTGAA+TGG 0.604172 3.3:-5172499 MS.gene63546:CDS
GAATGATCCTGATATCATGA+AGG 0.604709 3.3:-5173073 MS.gene63546:intron
TCGAGCGTTGATCGGACCCA+AGG 0.605088 3.3:-5171229 MS.gene63546:CDS
TACAGGAAGTAATTGACTCT+AGG 0.605900 3.3:-5173252 MS.gene63546:intron
AGAAGTTGATTCAATAGCTG+CGG 0.612265 3.3:+5171953 None:intergenic
TGTAAATGTCCTGGTGAAGG+TGG 0.614422 3.3:-5173123 MS.gene63546:CDS
AGAAATGCTTACGTGGCGAA+AGG 0.615480 3.3:-5174929 MS.gene63546:CDS
TGGAACTGATACCATTCTTG+AGG 0.619938 3.3:-5174902 MS.gene63546:intron
TACCATGGAGTTGATAAGGA+AGG 0.620910 3.3:-5174151 MS.gene63546:CDS
TCAACTCCATGGTAACCTTG+AGG 0.621566 3.3:+5174160 None:intergenic
GGATGTTAGAGATGCACGAG+AGG 0.621739 3.3:-5175283 MS.gene63546:CDS
CTTTCAGTTACACTCACTGA+AGG 0.625497 3.3:-5172853 MS.gene63546:intron
AGATAGCTGTTACTATCCTG+AGG 0.625896 3.3:-5173585 MS.gene63546:intron
ATGATTTACTGGAGAACATG+CGG 0.627372 3.3:-5171635 MS.gene63546:CDS
ACATCCTCAATAGCCACTGG+TGG 0.631828 3.3:+5175300 None:intergenic
TATTACCCTCAAGGTTACCA+TGG 0.632301 3.3:-5174166 MS.gene63546:CDS
TTGACCTTGAGAAGACAAAG+AGG 0.633659 3.3:-5171785 MS.gene63546:CDS
ATGGAGTTGATAAGGAAGGT+AGG 0.634219 3.3:-5174147 MS.gene63546:CDS
GCTTGTAGGACAAATTCAGA+AGG 0.637235 3.3:-5172367 MS.gene63546:intron
ACTCATCTCCTACCAGACAA+AGG 0.643406 3.3:-5172658 MS.gene63546:CDS
TCATGTAAATGTCCTGGTGA+AGG 0.645326 3.3:-5173126 MS.gene63546:CDS
TGACCTTGAGAAGACAAAGA+GGG 0.648508 3.3:-5171784 MS.gene63546:intron
ATGATTATCATACATTGCTG+AGG 0.651082 3.3:-5175197 MS.gene63546:intron
ATCGGACCCAAGGAAGCCCG+AGG 0.651998 3.3:-5171219 MS.gene63546:CDS
TTGGCGCAATTCAAGGACTG+CGG 0.653716 3.3:+5175257 None:intergenic
TCACAAACTGGTGCTAAACG+AGG 0.682295 3.3:+5172623 None:intergenic
ACAACAATACTGGATGTACA+GGG 0.693937 3.3:-5173962 MS.gene63546:CDS
TGTGGGAAGAAATGCTTACG+TGG 0.704198 3.3:-5174936 MS.gene63546:CDS
GGATGTCTTAGGTCTAACAA+GGG 0.722635 3.3:-5173102 MS.gene63546:CDS
TTAGCTATCAGGTAACGCGG+AGG 0.734505 3.3:-5172931 MS.gene63546:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTAAAATATTTATATATA+AGG - chr3.3:5174994-5175013 MS.gene63546:intron 0.0%
!!! TTTAAATTTTATAAAAAAAT+TGG + chr3.3:5171972-5171991 None:intergenic 0.0%
!! AAAATCAAAAAAAAAAATGA+AGG + chr3.3:5171784-5171803 None:intergenic 10.0%
!!! AAATTTTATAAAAAAATTGG+AGG + chr3.3:5171969-5171988 None:intergenic 10.0%
!! AAATAGTTTGAAAAAAAATG+AGG + chr3.3:5171404-5171423 None:intergenic 15.0%
!! TATTGTTATAAAATTGTTGA+AGG - chr3.3:5174226-5174245 MS.gene63546:intron 15.0%
!! AAAAAGGAAAATTGACTATA+GGG - chr3.3:5171245-5171264 MS.gene63546:CDS 20.0%
!! AGGAAAAAAAAAAAAGTTAG+TGG + chr3.3:5174396-5174415 None:intergenic 20.0%
!! CTTATGCTATCAATAATTAT+TGG + chr3.3:5172251-5172270 None:intergenic 20.0%
!! GAAATCTCTATATAAATTAC+AGG - chr3.3:5173301-5173320 MS.gene63546:intron 20.0%
!! GGAAAAAAAAAAAAGTTAGT+GGG + chr3.3:5174395-5174414 None:intergenic 20.0%
!!! ATGTTTCATTTTCTTCTAAT+TGG - chr3.3:5172325-5172344 MS.gene63546:intron 20.0%
!!! TGTCATAAATAAAAAGTGTT+AGG - chr3.3:5171515-5171534 MS.gene63546:intron 20.0%
!!! TTTTATAAAAAAATTGGAGG+TGG + chr3.3:5171966-5171985 None:intergenic 20.0%
! AGTAGATTGTTTATGTGTTT+AGG - chr3.3:5172750-5172769 MS.gene63546:intron 25.0%
! AGTCAAGAATCTGAAAATTA+GGG + chr3.3:5173280-5173299 None:intergenic 25.0%
! ATATAACTCTCTTATAAGCT+TGG + chr3.3:5172101-5172120 None:intergenic 25.0%
! ATCAGAATATCTACATTAGT+TGG + chr3.3:5173618-5173637 None:intergenic 25.0%
! ATGCTATCAATAATTATTGG+AGG + chr3.3:5172248-5172267 None:intergenic 25.0%
! CAAACCTGATATTAGTAATA+AGG + chr3.3:5172678-5172697 None:intergenic 25.0%
! CCTAAAAATCAATCTCTTTA+GGG - chr3.3:5172795-5172814 MS.gene63546:intron 25.0%
! CTAATTCTTATCTTAACACA+CGG - chr3.3:5173991-5174010 MS.gene63546:CDS 25.0%
! GAAAAAGGAAAATTGACTAT+AGG - chr3.3:5171244-5171263 MS.gene63546:CDS 25.0%
! GATTATAGTTATAGAGACTT+TGG + chr3.3:5172135-5172154 None:intergenic 25.0%
! GCTTGAAATTAATGATTTAC+TGG - chr3.3:5174924-5174943 MS.gene63546:CDS 25.0%
! GTTAAATAGAAACTAGAATG+CGG + chr3.3:5173367-5173386 None:intergenic 25.0%
! TAGTATTATTACCTCAAGAA+TGG + chr3.3:5171682-5171701 None:intergenic 25.0%
! TATAACTCTCTTATAAGCTT+GGG + chr3.3:5172100-5172119 None:intergenic 25.0%
! TATAGGTTTACAAACATATG+CGG + chr3.3:5175150-5175169 None:intergenic 25.0%
! TTCAAATTCAAAATCCTTCA+AGG + chr3.3:5172363-5172382 None:intergenic 25.0%
! TTTAAGTCATAGCCATAAAA+AGG - chr3.3:5171703-5171722 MS.gene63546:intron 25.0%
! TTTCAAATATCGATCTATTG+TGG + chr3.3:5172504-5172523 None:intergenic 25.0%
! TTTGATTAAGTTTGATCTGA+TGG - chr3.3:5174843-5174862 MS.gene63546:intron 25.0%
!! AGTGTTTACTATTTTATGGT+AGG - chr3.3:5173782-5173801 MS.gene63546:intron 25.0%
!! CAAAAAAGTTTCTTTGGAAA+CGG + chr3.3:5173023-5173042 None:intergenic 25.0%
!! CAATTTGCTTAGCATTATTA+AGG - chr3.3:5174435-5174454 MS.gene63546:intron 25.0%
!! GCAGTATAAGATTTGAATTT+TGG - chr3.3:5174536-5174555 MS.gene63546:intron 25.0%
!! GTTTAGGATTCTATGAAATA+TGG - chr3.3:5172766-5172785 MS.gene63546:intron 25.0%
!! TACGAGTGTTTACTATTTTA+TGG - chr3.3:5173778-5173797 MS.gene63546:intron 25.0%
!!! AAAATTTGTGTTTTTTCTGC+AGG - chr3.3:5174876-5174895 MS.gene63546:intron 25.0%
!!! ATATTCTGATTTTAGCTATC+AGG - chr3.3:5173628-5173647 MS.gene63546:CDS 25.0%
!!! ATTAGCACTTTTGTTGTTTA+AGG - chr3.3:5171465-5171484 MS.gene63546:intron 25.0%
!!! CAAAAAAGTTTCTACTTTTG+AGG + chr3.3:5175096-5175115 None:intergenic 25.0%
!!! CTTTTTTTTTTTTCCTTTCC+AGG - chr3.3:5174400-5174419 MS.gene63546:intron 25.0%
!!! TTAGCACTTTTGTTGTTTAA+GGG - chr3.3:5171466-5171485 MS.gene63546:intron 25.0%
AAAAAAAAGTTAGTGGGAAG+TGG + chr3.3:5174389-5174408 None:intergenic 30.0%
AAACTTACTTCTTCACAAAC+TGG + chr3.3:5173962-5173981 None:intergenic 30.0%
AACATTACCTTCATGATATC+AGG + chr3.3:5173507-5173526 None:intergenic 30.0%
AAGAAGTAAACTTGAAGAGA+AGG - chr3.3:5175065-5175084 MS.gene63546:intron 30.0%
ACATATCCCCTTGTAATATT+TGG - chr3.3:5174305-5174324 MS.gene63546:intron 30.0%
ACGCAACAAAAAAGTTTCTT+TGG + chr3.3:5173029-5173048 None:intergenic 30.0%
ACTCATTCACTTAAGAAAAG+AGG - chr3.3:5171222-5171241 MS.gene63546:CDS 30.0%
AGCACCTTATTACTAATATC+AGG - chr3.3:5172671-5172690 MS.gene63546:CDS 30.0%
ATATGGAGAATATTGCTTGT+AGG - chr3.3:5174189-5174208 MS.gene63546:CDS 30.0%
ATCATCACATAGAAACGAAA+AGG + chr3.3:5173053-5173072 None:intergenic 30.0%
ATGATTATCATACATTGCTG+AGG - chr3.3:5171373-5171392 MS.gene63546:intron 30.0%
CAATGCTTATATTTGGCATA+TGG + chr3.3:5172000-5172019 None:intergenic 30.0%
CACTTAAGAAAAGAGGAAAA+AGG - chr3.3:5171229-5171248 MS.gene63546:CDS 30.0%
CTCTATATACGATATGTGAA+AGG + chr3.3:5174275-5174294 None:intergenic 30.0%
CTGTATCTTTGCAATAGTTT+GGG + chr3.3:5173177-5173196 None:intergenic 30.0%
CTGTTAATTATTCTCTGTGT+CGG - chr3.3:5172702-5172721 MS.gene63546:CDS 30.0%
GAAAGGAACGCAAATATAAT+TGG + chr3.3:5174258-5174277 None:intergenic 30.0%
GAGTCAAGAATCTGAAAATT+AGG + chr3.3:5173281-5173300 None:intergenic 30.0%
GCATAGACAATGCTTATATT+TGG + chr3.3:5172007-5172026 None:intergenic 30.0%
GCCTAAAAATCAATCTCTTT+AGG - chr3.3:5172794-5172813 MS.gene63546:intron 30.0%
GGATAATTATCTATCCTTGA+AGG - chr3.3:5172346-5172365 MS.gene63546:intron 30.0%
GTAATGTCTGCAAATACAAA+CGG + chr3.3:5173079-5173098 None:intergenic 30.0%
GTCAAAATTGTTCTGTTCAT+TGG + chr3.3:5173695-5173714 None:intergenic 30.0%
GTCATTGCAAACTAATGATA+TGG - chr3.3:5174172-5174191 MS.gene63546:CDS 30.0%
TAATATTTGGACTTCGAAGT+TGG - chr3.3:5174318-5174337 MS.gene63546:intron 30.0%
TAATTGAAATGTGTGATGCA+GGG - chr3.3:5172908-5172927 MS.gene63546:CDS 30.0%
TATAAGGAACATACTTGAAG+AGG - chr3.3:5175010-5175029 MS.gene63546:intron 30.0%
TCATCACATAGAAACGAAAA+GGG + chr3.3:5173052-5173071 None:intergenic 30.0%
TCGAAGTCCAAATATTACAA+GGG + chr3.3:5174315-5174334 None:intergenic 30.0%
TGAGAAAACAATTGAGATGT+GGG - chr3.3:5171617-5171636 MS.gene63546:intron 30.0%
TGTATCTTTGCAATAGTTTG+GGG + chr3.3:5173176-5173195 None:intergenic 30.0%
TTCGAAGTCCAAATATTACA+AGG + chr3.3:5174316-5174335 None:intergenic 30.0%
TTGAGAAAACAATTGAGATG+TGG - chr3.3:5171616-5171635 MS.gene63546:intron 30.0%
! AAAGCAAATCTGCCTTTTTA+TGG + chr3.3:5171718-5171737 None:intergenic 30.0%
! CAATAAGATTGTCATTTGCA+AGG + chr3.3:5173816-5173835 None:intergenic 30.0%
! CCATACATATTTTCAAAAGC+TGG + chr3.3:5173564-5173583 None:intergenic 30.0%
! CTTTTGGTCATGAAAAAGTT+GGG - chr3.3:5173751-5173770 MS.gene63546:intron 30.0%
! TAAAAAGGCAGATTTGCTTT+AGG - chr3.3:5171718-5171737 MS.gene63546:intron 30.0%
! TCCTTTAAAAGTCAGATATC+AGG - chr3.3:5175305-5175324 MS.gene63546:CDS 30.0%
! TCTTTTGGTCATGAAAAAGT+TGG - chr3.3:5173750-5173769 MS.gene63546:intron 30.0%
!! CCAGCTTTTGAAAATATGTA+TGG - chr3.3:5173561-5173580 MS.gene63546:intron 30.0%
!! CCCTAAAGAGATTGATTTTT+AGG + chr3.3:5172798-5172817 None:intergenic 30.0%
!! GATTTTTAACTAAGCTACCT+TGG - chr3.3:5171427-5171446 MS.gene63546:intron 30.0%
!! TTTTTCGACAACAACAATAC+TGG - chr3.3:5172598-5172617 MS.gene63546:intron 30.0%
AACAACAATACTGGATGTAC+AGG - chr3.3:5172607-5172626 MS.gene63546:intron 35.0%
ACAACAATACTGGATGTACA+GGG - chr3.3:5172608-5172627 MS.gene63546:intron 35.0%
ACAAGGGGATATGTACAATT+TGG + chr3.3:5174299-5174318 None:intergenic 35.0%
ACATGAACCACCAAGAAAAT+CGG + chr3.3:5173430-5173449 None:intergenic 35.0%
ACTAGAATGCGGTATAATGA+AGG + chr3.3:5173356-5173375 None:intergenic 35.0%
AGCTCATACTTGTACATGAA+TGG - chr3.3:5171882-5171901 MS.gene63546:CDS 35.0%
AGCTTAGCATGAATATTAGC+CGG + chr3.3:5174353-5174372 None:intergenic 35.0%
ATAACTACATCTCCTTTGTC+TGG + chr3.3:5173927-5173946 None:intergenic 35.0%
ATCCAAAAAACAGTTCTCCA+AGG + chr3.3:5171447-5171466 None:intergenic 35.0%
ATGATATCAGGATCATTCCA+TGG + chr3.3:5173495-5173514 None:intergenic 35.0%
ATGATTTACTGGAGAACATG+CGG - chr3.3:5174935-5174954 MS.gene63546:CDS 35.0%
CAAATGTACATTGTCAATGC+TGG - chr3.3:5173101-5173120 MS.gene63546:CDS 35.0%
CAAGAGCTATTTATGCTTGA+TGG + chr3.3:5172200-5172219 None:intergenic 35.0%
CCAAACTATTGCAAAGATAC+AGG - chr3.3:5173175-5173194 MS.gene63546:intron 35.0%
CCTGTATCTTTGCAATAGTT+TGG + chr3.3:5173178-5173197 None:intergenic 35.0%
CGAAGTCCAAATATTACAAG+GGG + chr3.3:5174314-5174333 None:intergenic 35.0%
CGACACAGAGAATAATTAAC+AGG + chr3.3:5172704-5172723 None:intergenic 35.0%
GAACTCAATAACAAACCTGA+TGG - chr3.3:5174700-5174719 MS.gene63546:intron 35.0%
GAATGATCCTGATATCATGA+AGG - chr3.3:5173497-5173516 MS.gene63546:intron 35.0%
GCTCATACTTGTACATGAAT+GGG - chr3.3:5171883-5171902 MS.gene63546:CDS 35.0%
GGGTTGTTGATACAGTATTA+GGG - chr3.3:5171903-5171922 MS.gene63546:CDS 35.0%
GGTGTAACAGAAGGATAATT+AGG - chr3.3:5171755-5171774 MS.gene63546:intron 35.0%
GTAATTGAAATGTGTGATGC+AGG - chr3.3:5172907-5172926 MS.gene63546:CDS 35.0%
GTTTCAACAACTTGCAAACA+AGG + chr3.3:5172864-5172883 None:intergenic 35.0%
TACCTTGTCAAACTAGCATT+TGG - chr3.3:5173592-5173611 MS.gene63546:CDS 35.0%
TATTATTCTTGCAGCAGAGA+AGG - chr3.3:5175247-5175266 MS.gene63546:CDS 35.0%
TATTGCAAAGATACAGGTGA+AGG - chr3.3:5173181-5173200 MS.gene63546:intron 35.0%
TCAAGCATAAATAGCTCTTG+CGG - chr3.3:5172200-5172219 MS.gene63546:intron 35.0%
TCTTGTTGATCTGTATTTCC+CGG - chr3.3:5171564-5171583 MS.gene63546:intron 35.0%
TGATAATCATCATGCCTAGA+AGG + chr3.3:5171363-5171382 None:intergenic 35.0%
TGGGTTGTTGATACAGTATT+AGG - chr3.3:5171902-5171921 MS.gene63546:CDS 35.0%
! AAAGTTTCTTTGGAAACGGA+CGG + chr3.3:5173019-5173038 None:intergenic 35.0%
! AACGGACTTTATTCTATCCA+AGG + chr3.3:5174761-5174780 None:intergenic 35.0%
! AACTATTTTCTCATCTCCCA+GGG - chr3.3:5173842-5173861 MS.gene63546:intron 35.0%
! AGAAGTTGATTCAATAGCTG+CGG + chr3.3:5174620-5174639 None:intergenic 35.0%
! ATGCTTGATGGTATGCAAAA+TGG + chr3.3:5172188-5172207 None:intergenic 35.0%
! CTGATGATGTTGCAACTTAT+AGG + chr3.3:5175167-5175186 None:intergenic 35.0%
! GAAGGCAAGTTTAAGTCTTT+TGG - chr3.3:5173735-5173754 MS.gene63546:intron 35.0%
! GCCTGATATCTGACTTTTAA+AGG + chr3.3:5175309-5175328 None:intergenic 35.0%
! GGATTTTGAATTTGAAGAGC+TGG - chr3.3:5172367-5172386 MS.gene63546:intron 35.0%
! TAACTATTTTCTCATCTCCC+AGG - chr3.3:5173841-5173860 MS.gene63546:intron 35.0%
! TACAGGAAGTAATTGACTCT+AGG - chr3.3:5173318-5173337 MS.gene63546:intron 35.0%
! TGCTTGATGGTATGCAAAAT+GGG + chr3.3:5172187-5172206 None:intergenic 35.0%
! TTAAGTTTGATCTGATGGTG+TGG - chr3.3:5174848-5174867 MS.gene63546:intron 35.0%
!! TCTAGCTTTAAGAAACCTAC+CGG + chr3.3:5171586-5171605 None:intergenic 35.0%
!!! ACAGCTATCTATTTTAGCCA+TGG + chr3.3:5172975-5172994 None:intergenic 35.0%
!!! ATTTTAGCTATCAGGTAACG+CGG - chr3.3:5173636-5173655 MS.gene63546:CDS 35.0%
!!! TACCTTGGAGAACTGTTTTT+TGG - chr3.3:5171442-5171461 MS.gene63546:intron 35.0%
AGATAGCTGTTACTATCCTG+AGG - chr3.3:5172985-5173004 MS.gene63546:intron 40.0%
ATACCCTCTTTGTCTTCTCA+AGG + chr3.3:5174792-5174811 None:intergenic 40.0%
ATGTACAAGTATGAGCTGTG+AGG + chr3.3:5171879-5171898 None:intergenic 40.0%
CAAGCATAAATAGCTCTTGC+GGG - chr3.3:5172201-5172220 MS.gene63546:intron 40.0%
CATGAACCACCAAGAAAATC+GGG + chr3.3:5173429-5173448 None:intergenic 40.0%
CTCTCTCAAACTCTTGTACA+TGG + chr3.3:5172529-5172548 None:intergenic 40.0%
CTTATAAGCTTGGGCTGTTA+AGG + chr3.3:5172091-5172110 None:intergenic 40.0%
CTTCAGTGAGTGTAACTGAA+AGG + chr3.3:5173719-5173738 None:intergenic 40.0%
CTTCTCAAGGTCAAACTCAA+CGG + chr3.3:5174779-5174798 None:intergenic 40.0%
CTTTAGGCCACAGAATCTTT+CGG - chr3.3:5171734-5171753 MS.gene63546:intron 40.0%
CTTTCAGTTACACTCACTGA+AGG - chr3.3:5173717-5173736 MS.gene63546:intron 40.0%
GATCAACTTACTCGACACTT+CGG + chr3.3:5174964-5174983 None:intergenic 40.0%
GCAAATGCTTATGGAATCCT+TGG - chr3.3:5174741-5174760 MS.gene63546:intron 40.0%
GCTTGTAGGACAAATTCAGA+AGG - chr3.3:5174203-5174222 MS.gene63546:CDS 40.0%
GGATGTCTTAGGTCTAACAA+GGG - chr3.3:5173468-5173487 MS.gene63546:CDS 40.0%
GTATTGCAGTATTACCCTCA+AGG - chr3.3:5172395-5172414 MS.gene63546:CDS 40.0%
GTTAATAATGACGCGTTCTG+TGG + chr3.3:5174593-5174612 None:intergenic 40.0%
TACATTGTCAATGCTGGTAC+TGG - chr3.3:5173107-5173126 MS.gene63546:CDS 40.0%
TACCTTCCTTATCAACTCCA+TGG + chr3.3:5172424-5172443 None:intergenic 40.0%
TATAAGATCAGTAACCTCGC+AGG + chr3.3:5173250-5173269 None:intergenic 40.0%
TATTACCCTCAAGGTTACCA+TGG - chr3.3:5172404-5172423 MS.gene63546:CDS 40.0%
TCATGTAAATGTCCTGGTGA+AGG - chr3.3:5173444-5173463 MS.gene63546:CDS 40.0%
TCGAAGTTGGAAATACTTGC+CGG - chr3.3:5174331-5174350 MS.gene63546:intron 40.0%
TGACCTTGAGAAGACAAAGA+GGG - chr3.3:5174786-5174805 MS.gene63546:intron 40.0%
TGAGTGTATGACTAAGCAAG+TGG - chr3.3:5171931-5171950 MS.gene63546:CDS 40.0%
TGGAACTGATACCATTCTTG+AGG - chr3.3:5171668-5171687 MS.gene63546:CDS 40.0%
TGGATGTCTTAGGTCTAACA+AGG - chr3.3:5173467-5173486 MS.gene63546:CDS 40.0%
TGTTACACCGAAAGATTCTG+TGG + chr3.3:5171744-5171763 None:intergenic 40.0%
TTGACCTTGAGAAGACAAAG+AGG - chr3.3:5174785-5174804 MS.gene63546:intron 40.0%
TTGATTCAATAGCTGCGGAA+TGG + chr3.3:5174615-5174634 None:intergenic 40.0%
! AAGTTTCTTTGGAAACGGAC+GGG + chr3.3:5173018-5173037 None:intergenic 40.0%
! AATCTCTTTAGGGACTAGTG+AGG - chr3.3:5172805-5172824 MS.gene63546:intron 40.0%
! ACCTCAAGCAACAGTTGTTT+TGG + chr3.3:5172036-5172055 None:intergenic 40.0%
! ACTATTTTCTCATCTCCCAG+GGG - chr3.3:5173843-5173862 MS.gene63546:intron 40.0%
! ATCTCTTTAGGGACTAGTGA+GGG - chr3.3:5172806-5172825 MS.gene63546:intron 40.0%
! GAATCTTTCGGTGTAACAGA+AGG - chr3.3:5171746-5171765 MS.gene63546:intron 40.0%
! TTTTGTGAGGCAAATCACCA+GGG - chr3.3:5173666-5173685 MS.gene63546:intron 40.0%
!! AGGTTACCATGGAGTTGATA+AGG - chr3.3:5172415-5172434 MS.gene63546:CDS 40.0%
!! ATGGAGTTGATAAGGAAGGT+AGG - chr3.3:5172423-5172442 MS.gene63546:CDS 40.0%
!! CTAGCTTTAAGAAACCTACC+GGG + chr3.3:5171585-5171604 None:intergenic 40.0%
!! TACCATGGAGTTGATAAGGA+AGG - chr3.3:5172419-5172438 MS.gene63546:CDS 40.0%
!! TTTTTCCTTTCCAGGTACTG+CGG - chr3.3:5174408-5174427 MS.gene63546:intron 40.0%
AAAGGGGATCTTTGCCTTCT+AGG - chr3.3:5171346-5171365 MS.gene63546:intron 45.0%
AATACTGGATGTACAGGGCT+TGG - chr3.3:5172613-5172632 MS.gene63546:intron 45.0%
AATCTGTCTCTTCGCTGCAA+TGG + chr3.3:5172579-5172598 None:intergenic 45.0%
ACGCGTTCTGTGGAATTTGA+TGG + chr3.3:5174583-5174602 None:intergenic 45.0%
ACGTCTTCAGAGGCTAGAAA+AGG - chr3.3:5174669-5174688 MS.gene63546:intron 45.0%
ACTCATCTCCTACCAGACAA+AGG - chr3.3:5173912-5173931 MS.gene63546:intron 45.0%
AGAAATGCTTACGTGGCGAA+AGG - chr3.3:5171641-5171660 MS.gene63546:CDS 45.0%
AGCAAATCTCTTGGCTTCCA+TGG - chr3.3:5172955-5172974 MS.gene63546:intron 45.0%
AGCATTTGCTCCTTCTCCAA+AGG + chr3.3:5174730-5174749 None:intergenic 45.0%
ATAAGCTTGGGCTGTTAAGG+TGG + chr3.3:5172088-5172107 None:intergenic 45.0%
ATCCCCTTTCGACAAGTCTT+TGG + chr3.3:5171335-5171354 None:intergenic 45.0%
ATGAAGGATGTCAGCACTTG+TGG + chr3.3:5174493-5174512 None:intergenic 45.0%
CAACTCCATGGTAACCTTGA+GGG + chr3.3:5172412-5172431 None:intergenic 45.0%
CAAGTCTTTGGCGCAATTCA+AGG + chr3.3:5171323-5171342 None:intergenic 45.0%
CCAAGCCTCTCAATGTAAAC+CGG + chr3.3:5172449-5172468 None:intergenic 45.0%
CGCGTTCTGTGGAATTTGAT+GGG + chr3.3:5174582-5174601 None:intergenic 45.0%
CGGTTTACATTGAGAGGCTT+GGG - chr3.3:5172447-5172466 MS.gene63546:CDS 45.0%
CTAACATCCTCAATAGCCAC+TGG + chr3.3:5171276-5171295 None:intergenic 45.0%
CTACATCTCCTTTGTCTGGT+AGG + chr3.3:5173923-5173942 None:intergenic 45.0%
CTGTAGTAGCCATTCACATC+TGG + chr3.3:5174083-5174102 None:intergenic 45.0%
CTTGTGAACAACGTCTTCAG+AGG - chr3.3:5174659-5174678 MS.gene63546:intron 45.0%
GCGGTATAATGAAGGAACAG+AGG + chr3.3:5173348-5173367 None:intergenic 45.0%
GGAGAAGGAGCAAATGCTTA+TGG - chr3.3:5174732-5174751 MS.gene63546:intron 45.0%
GGTGGTTCATGTAAATGTCC+TGG - chr3.3:5173438-5173457 MS.gene63546:CDS 45.0%
GTTGATCTGTATTTCCCGGT+AGG - chr3.3:5171568-5171587 MS.gene63546:intron 45.0%
TACAAGTATGAGCTGTGAGG+TGG + chr3.3:5171876-5171895 None:intergenic 45.0%
TCAAAAGCTGGCGCAAGTAA+GGG + chr3.3:5173552-5173571 None:intergenic 45.0%
TCAACTCCATGGTAACCTTG+AGG + chr3.3:5172413-5172432 None:intergenic 45.0%
TCTATGAACTTCTGTGCAGC+AGG + chr3.3:5173152-5173171 None:intergenic 45.0%
TGTAAATGTCCTGGTGAAGG+TGG - chr3.3:5173447-5173466 MS.gene63546:CDS 45.0%
TGTGGGAAGAAATGCTTACG+TGG - chr3.3:5171634-5171653 MS.gene63546:CDS 45.0%
TTAGGTCTAACAAGGGTCCA+TGG - chr3.3:5173475-5173494 MS.gene63546:CDS 45.0%
TTCAAAAGCTGGCGCAAGTA+AGG + chr3.3:5173553-5173572 None:intergenic 45.0%
! CTCTGAAGACGTTGTTCACA+AGG + chr3.3:5174661-5174680 None:intergenic 45.0%
! CTTTTGTGAGGCAAATCACC+AGG - chr3.3:5173665-5173684 MS.gene63546:intron 45.0%
! GCCAAAACAACTGTTGCTTG+AGG - chr3.3:5172032-5172051 MS.gene63546:CDS 45.0%
! GGCCAAATGCTAGTTTGACA+AGG + chr3.3:5173597-5173616 None:intergenic 45.0%
! TCACAAACTGGTGCTAAACG+AGG + chr3.3:5173950-5173969 None:intergenic 45.0%
! TCTCTTTAGGGACTAGTGAG+GGG - chr3.3:5172807-5172826 MS.gene63546:intron 45.0%
!! ACAAGTGCTGACATCCTTCA+TGG - chr3.3:5174492-5174511 MS.gene63546:intron 45.0%
!! AGTTGATAAGGAAGGTAGGC+CGG - chr3.3:5172427-5172446 MS.gene63546:CDS 45.0%
!! CTGGCTTTAGGAAGATGCTT+TGG - chr3.3:5173126-5173145 MS.gene63546:CDS 45.0%
!! TCAATGCTGGTACTGGCTTT+AGG - chr3.3:5173114-5173133 MS.gene63546:CDS 45.0%
AAATTGCTGACCGCAGTACC+TGG + chr3.3:5174421-5174440 None:intergenic 50.0%
AAGTATGAGCTGTGAGGTGG+AGG + chr3.3:5171873-5171892 None:intergenic 50.0%
ACATCCTCAATAGCCACTGG+TGG + chr3.3:5171273-5171292 None:intergenic 50.0%
ACCTGCAAAAGACAGCGGTT+AGG + chr3.3:5174053-5174072 None:intergenic 50.0%
ACGGACGGGATAAAAACCTC+AGG + chr3.3:5173004-5173023 None:intergenic 50.0%
AGCGACTCTTCAGCAGAATC+AGG - chr3.3:5173872-5173891 MS.gene63546:intron 50.0%
CAAACCTGATGGCATGCCTT+TGG - chr3.3:5174711-5174730 MS.gene63546:intron 50.0%
CACCAAGAAAATCGGGCAAC+TGG + chr3.3:5173422-5173441 None:intergenic 50.0%
CCGGTTTACATTGAGAGGCT+TGG - chr3.3:5172446-5172465 MS.gene63546:CDS 50.0%
CGCCAAAGACTTGTCGAAAG+GGG - chr3.3:5171330-5171349 MS.gene63546:intron 50.0%
CTAAGACATCCACCTTCACC+AGG + chr3.3:5173459-5173478 None:intergenic 50.0%
CTGGTGAAGGTGGATGTCTT+AGG - chr3.3:5173457-5173476 MS.gene63546:CDS 50.0%
CTTACGTGGCGAAAGGAGTA+TGG - chr3.3:5171648-5171667 MS.gene63546:CDS 50.0%
GCAATGACTGCTCACGAGTA+AGG + chr3.3:5174160-5174179 None:intergenic 50.0%
GCATAAATAGCTCTTGCGGG+AGG - chr3.3:5172204-5172223 MS.gene63546:intron 50.0%
GCGCCAAAGACTTGTCGAAA+GGG - chr3.3:5171329-5171348 MS.gene63546:intron 50.0%
GCGTACTGCAGCAAATCTCT+TGG - chr3.3:5172946-5172965 MS.gene63546:intron 50.0%
GGATGTTAGAGATGCACGAG+AGG - chr3.3:5171287-5171306 MS.gene63546:intron 50.0%
GTGATGTGCATCAAGCGACT+TGG + chr3.3:5172482-5172501 None:intergenic 50.0%
TAAATAGCTCTTGCGGGAGG+CGG - chr3.3:5172207-5172226 MS.gene63546:intron 50.0%
TAGACTTGAATGCCTCGCCT+CGG + chr3.3:5175371-5175390 None:intergenic 50.0%
TGACTATAGGGTTCCACCAG+TGG - chr3.3:5171257-5171276 MS.gene63546:intron 50.0%
TGCGCCAAAGACTTGTCGAA+AGG - chr3.3:5171328-5171347 MS.gene63546:intron 50.0%
TTACATGCTCTTCTCCTGCG+AGG - chr3.3:5173233-5173252 MS.gene63546:intron 50.0%
TTAGCTATCAGGTAACGCGG+AGG - chr3.3:5173639-5173658 MS.gene63546:CDS 50.0%
TTCGCTGCAATGGAACATGC+TGG + chr3.3:5172569-5172588 None:intergenic 50.0%
TTGGCGCAATTCAAGGACTG+CGG + chr3.3:5171316-5171335 None:intergenic 50.0%
! ACCTAACCGCTGTCTTTTGC+AGG - chr3.3:5174049-5174068 MS.gene63546:CDS 50.0%
! AGCCAGTTGCCCGATTTTCT+TGG - chr3.3:5173417-5173436 MS.gene63546:CDS 50.0%
! CACGGCATGTGCATTTTGTG+TGG - chr3.3:5174009-5174028 MS.gene63546:CDS 50.0%
! CAGTTGCCCGATTTTCTTGG+TGG - chr3.3:5173420-5173439 MS.gene63546:CDS 50.0%
! CTCTTTAGGGACTAGTGAGG+GGG - chr3.3:5172808-5172827 MS.gene63546:intron 50.0%
! GTAGGCCGGTTTACATTGAG+AGG - chr3.3:5172441-5172460 MS.gene63546:CDS 50.0%
! GTCAGGGTACCAGATGTGAA+TGG - chr3.3:5174071-5174090 MS.gene63546:CDS 50.0%
! TGATGGCATGCCTTTGGAGA+AGG - chr3.3:5174717-5174736 MS.gene63546:intron 50.0%
! TTCTCCAAAGGCATGCCATC+AGG + chr3.3:5174718-5174737 None:intergenic 50.0%
AGACTTGAATGCCTCGCCTC+GGG + chr3.3:5175370-5175389 None:intergenic 55.0%
CCCTGACCTGCAAAAGACAG+CGG + chr3.3:5174058-5174077 None:intergenic 55.0%
CGCAGCGAGTCTTTCCATGA+AGG + chr3.3:5174509-5174528 None:intergenic 55.0%
GGTTCCACCAGTGGCTATTG+AGG - chr3.3:5171266-5171285 MS.gene63546:intron 55.0%
GTTCTGTTCATTGGACGCCC+TGG + chr3.3:5173686-5173705 None:intergenic 55.0%
! ACCGCTGTCTTTTGCAGGTC+AGG - chr3.3:5174054-5174073 MS.gene63546:CDS 55.0%
! ACGCGGAGGCAACTTTTGTG+AGG - chr3.3:5173653-5173672 MS.gene63546:intron 55.0%
! CCGCTGTCTTTTGCAGGTCA+GGG - chr3.3:5174055-5174074 MS.gene63546:CDS 55.0%
! GGACTAGTGAGGGGGTTCTT+TGG - chr3.3:5172816-5172835 MS.gene63546:intron 55.0%
!! TCGCACAGTCGAGCGTTGAT+CGG - chr3.3:5175333-5175352 MS.gene63546:CDS 55.0%
GCTGACCGCAGTACCTGGAA+AGG + chr3.3:5174416-5174435 None:intergenic 60.0%
!! TCGAGCGTTGATCGGACCCA+AGG - chr3.3:5175341-5175360 MS.gene63546:CDS 60.0%
AAGAGTCGCTGCATCGCCCC+TGG + chr3.3:5173862-5173881 None:intergenic 65.0%
AGAGTCGCTGCATCGCCCCT+GGG + chr3.3:5173861-5173880 None:intergenic 65.0%
ATCGGACCCAAGGAAGCCCG+AGG - chr3.3:5175351-5175370 MS.gene63546:CDS 65.0%
ACCCAAGGAAGCCCGAGGCG+AGG - chr3.3:5175356-5175375 MS.gene63546:CDS 70.0%
GCCTCGCCTCGGGCTTCCTT+GGG + chr3.3:5175360-5175379 None:intergenic 70.0%
TGCCTCGCCTCGGGCTTCCT+TGG + chr3.3:5175361-5175380 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 5171201 5175391 5171201 ID=MS.gene63546
chr3.3 mRNA 5171201 5175391 5171201 ID=MS.gene63546.t1;Parent=MS.gene63546
chr3.3 exon 5175198 5175391 5175198 ID=MS.gene63546.t1.exon1;Parent=MS.gene63546.t1
chr3.3 CDS 5175198 5175391 5175198 ID=cds.MS.gene63546.t1;Parent=MS.gene63546.t1
chr3.3 exon 5174903 5175002 5174903 ID=MS.gene63546.t1.exon2;Parent=MS.gene63546.t1
chr3.3 CDS 5174903 5175002 5174903 ID=cds.MS.gene63546.t1;Parent=MS.gene63546.t1
chr3.3 exon 5173958 5174224 5173958 ID=MS.gene63546.t1.exon3;Parent=MS.gene63546.t1
chr3.3 CDS 5173958 5174224 5173958 ID=cds.MS.gene63546.t1;Parent=MS.gene63546.t1
chr3.3 exon 5173586 5173663 5173586 ID=MS.gene63546.t1.exon4;Parent=MS.gene63546.t1
chr3.3 CDS 5173586 5173663 5173586 ID=cds.MS.gene63546.t1;Parent=MS.gene63546.t1
chr3.3 exon 5173396 5173500 5173396 ID=MS.gene63546.t1.exon5;Parent=MS.gene63546.t1
chr3.3 CDS 5173396 5173500 5173396 ID=cds.MS.gene63546.t1;Parent=MS.gene63546.t1
chr3.3 exon 5173253 5173302 5173253 ID=MS.gene63546.t1.exon6;Parent=MS.gene63546.t1
chr3.3 CDS 5173253 5173302 5173253 ID=cds.MS.gene63546.t1;Parent=MS.gene63546.t1
chr3.3 exon 5173074 5173173 5173074 ID=MS.gene63546.t1.exon7;Parent=MS.gene63546.t1
chr3.3 CDS 5173074 5173173 5173074 ID=cds.MS.gene63546.t1;Parent=MS.gene63546.t1
chr3.3 exon 5172854 5172949 5172854 ID=MS.gene63546.t1.exon8;Parent=MS.gene63546.t1
chr3.3 CDS 5172854 5172949 5172854 ID=cds.MS.gene63546.t1;Parent=MS.gene63546.t1
chr3.3 exon 5172619 5172729 5172619 ID=MS.gene63546.t1.exon9;Parent=MS.gene63546.t1
chr3.3 CDS 5172619 5172729 5172619 ID=cds.MS.gene63546.t1;Parent=MS.gene63546.t1
chr3.3 exon 5172368 5172521 5172368 ID=MS.gene63546.t1.exon10;Parent=MS.gene63546.t1
chr3.3 CDS 5172368 5172521 5172368 ID=cds.MS.gene63546.t1;Parent=MS.gene63546.t1
chr3.3 exon 5171785 5172170 5171785 ID=MS.gene63546.t1.exon11;Parent=MS.gene63546.t1
chr3.3 CDS 5171785 5172170 5171785 ID=cds.MS.gene63546.t1;Parent=MS.gene63546.t1
chr3.3 exon 5171620 5171694 5171620 ID=MS.gene63546.t1.exon12;Parent=MS.gene63546.t1
chr3.3 CDS 5171620 5171694 5171620 ID=cds.MS.gene63546.t1;Parent=MS.gene63546.t1
chr3.3 exon 5171201 5171272 5171201 ID=MS.gene63546.t1.exon13;Parent=MS.gene63546.t1
chr3.3 CDS 5171201 5171272 5171201 ID=cds.MS.gene63546.t1;Parent=MS.gene63546.t1
Gene Sequence

>MS.gene63546

ATGAATGCTTCTTCTAAATTCACTCATTCACTTAAGAAAAGAGGAAAAAGGAAAATTGACTATAGGGTTCCACCAGTGGCTATTGAGGATGTTAGAGATGCACGAGAGGAGACCGCAGTCCTTGAATTGCGCCAAAGACTTGTCGAAAGGGGATCTTTGCCTTCTAGGCATGATGATTATCATACATTGCTGAGGTACTTCCTCATTTTTTTTCAAACTATTTCGCGATTTTTAACTAAGCTACCTTGGAGAACTGTTTTTTGGATTAGCACTTTTGTTGTTTAAGGGCATACTCACTTGCATTTTAAGTGTGTTGTCATAAATAAAAAGTGTTAGGTTTTTATTTGCTCACATAACGTTGATTCTTGTTGATCTGTATTTCCCGGTAGGTTTCTTAAAGCTAGAGATTTTAATATTGAGAAAACAATTGAGATGTGGGAAGAAATGCTTACGTGGCGAAAGGAGTATGGAACTGATACCATTCTTGAGGTAATAATACTAATTTAAGTCATAGCCATAAAAAGGCAGATTTGCTTTAGGCCACAGAATCTTTCGGTGTAACAGAAGGATAATTAGGATTCCTTCATTTTTTTTTTTGATTTTGTCGATCGTAAATTCAATACATTTGTTATTTAATTTGAACAAAGCTGATATGTAATCATTCACTAGCCTCCACCTCACAGCTCATACTTGTACATGAATGGGTTGTTGATACAGTATTAGGGATGTATGAGTGTATGACTAAGCAAGTGGTCAAGAGTTCCACCTCCAATTTTTTTATAAAATTTAAATCTCTCCATATGCCAAATATAAGCATTGTCTATGCATCAAGCCAAAACAACTGTTGCTTGAGGTGTGTTAGATATAAAATCACTGACGAGTCTCCACCTTAACAGCCCAAGCTTATAAGAGAGTTATATTTGTCGTAGTACCAAAGTCTCTATAACTATAATCTAGTAGTTATGAGTTCAATGCTTGCTACTCCCATTTTGCATACCATCAAGCATAAATAGCTCTTGCGGGAGGCGGAATGTTAGATAAAAACCTCCAATAATTATTGATAGCATAAGCAAATAAGAGCAAAATATTTTACTTTTGTAATATTTGAAAATTTTGCTTTATACATGTTTCATTTTCTTCTAATTGGATAATTATCTATCCTTGAAGGATTTTGAATTTGAAGAGCTGGAAGAAGTATTGCAGTATTACCCTCAAGGTTACCATGGAGTTGATAAGGAAGGTAGGCCGGTTTACATTGAGAGGCTTGGGAAAGCTCATCCAAGTCGCTTGATGCACATCACCACAATAGATCGATATTTGAAATACCATGTACAAGAGTTTGAGAGAGCGTTGCAAGAGAAATTTCCAGCATGTTCCATTGCAGCGAAGAGACAGATTTTTTCGACAACAACAATACTGGATGTACAGGGCTTGGTATGTTGCATTACACTAATAATTTTTATAAAGTTAAGCACCTTATTACTAATATCAGGTTTGAAACCTGTTAATTATTCTCTGTGTCGGATAGTTTGCTAAGCTGCTTGTTGATAGTAGATTGTTTATGTGTTTAGGATTCTATGAAATATGGTATCTGCCTAAAAATCAATCTCTTTAGGGACTAGTGAGGGGGTTCTTTGGTGTTTCTCAATTTTAAGAATTTCCTTGTTTGCAAGTTGTTGAAACGTTTTTATGCTGAAATTTTAGCTGTAATTGAAATGTGTGATGCAGGGAATGAAAAATTTCTCGCGTACTGCAGCAAATCTCTTGGCTTCCATGGCTAAAATAGATAGCTGTTACTATCCTGAGGTTTTTATCCCGTCCGTTTCCAAAGAAACTTTTTTGTTGCGTCCCTTTTCGTTTCTATGTGATGATTATCCGTTTGTATTTGCAGACATTACATCAAATGTACATTGTCAATGCTGGTACTGGCTTTAGGAAGATGCTTTGGCCTGCTGCACAGAAGTTCATAGATCCCCAAACTATTGCAAAGATACAGGTGAAGGATATAGCTATCATATCAGTCAGCAATATTTTACATGCTCTTCTCCTGCGAGGTTACTGATCTTATATTCATTTCCCTAATTTTCAGATTCTTGACTCGAAATCTCTATATAAATTACAGGAAGTAATTGACTCTAGGTACGCCTCTGTTCCTTCATTATACCGCATTCTAGTTTCTATTTAACTTACTGATTAGTTTGTAAATGAATCTGCACAGCCAGTTGCCCGATTTTCTTGGTGGTTCATGTAAATGTCCTGGTGAAGGTGGATGTCTTAGGTCTAACAAGGGTCCATGGAATGATCCTGATATCATGAAGGTAATGTTTCTTTGTTATCTTGTTTCTTAGCCCTTACTTGCGCCAGCTTTTGAAAATATGTATGGTGTAGAAATACCTTGTCAAACTAGCATTTGGCCAACTAATGTAGATATTCTGATTTTAGCTATCAGGTAACGCGGAGGCAACTTTTGTGAGGCAAATCACCAGGGCGTCCAATGAACAGAACAATTTTGACTCCTTTCAGTTACACTCACTGAAGGCAAGTTTAAGTCTTTTGGTCATGAAAAAGTTGGGATTTTACGAGTGTTTACTATTTTATGGTAGGTAAACATTCCTTGCAAATGACAATCTTATTGTTTTTTAACTATTTTCTCATCTCCCAGGGGCGATGCAGCGACTCTTCAGCAGAATCAGGTTCTGATTTTAACGATTACTCATCTCCTACCAGACAAAGGAGATGTAGTTATCCTCGTTTAGCACCAGTTTGTGAAGAAGTAAGTTTTTCAAAATTCTAATTCTTATCTTAACACACGGCATGTGCATTTTGTGTGGTTGTCGCTTTTAATCATACCTAACCGCTGTCTTTTGCAGGTCAGGGTACCAGATGTGAATGGCTACTACAGCTGTGATGATAGTGCTCTATCAGCTCAGAATGTAATAGAAAATGATCATCATCGCCTTACTCGTGAGCAGTCATTGCAAACTAATGATATGGAGAATATTGCTTGTAGGACAAATTCAGAAGGTATTGTTATAAAATTGTTGAAGGCTTGAACCAATTATATTTGCGTTCCTTTCACATATCGTATATAGAGTCCAAATTGTACATATCCCCTTGTAATATTTGGACTTCGAAGTTGGAAATACTTGCCGGCTAATATTCATGCTAAGCTGTTTTATCTTTATCCACTTCCCACTAACTTTTTTTTTTTTCCTTTCCAGGTACTGCGGTCAGCAATTTGCTTAGCATTATTAAGGAGAAATTAGAGAGAATAAATTTCTTATATGTACCACAAGTGCTGACATCCTTCATGGAAAGACTCGCTGCGTTCATCTGCAGTATAAGATTTGAATTTTGGAGAACACAGAACATTGTTCACCCATCAAATTCCACAGAACGCGTCATTATTAACCATTCCGCAGCTATTGAATCAACTTCTGAAAGAGATTATATTCTTCCTTGTGAACAACGTCTTCAGAGGCTAGAAAAGGTTTTTAATGAACTCAATAACAAACCTGATGGCATGCCTTTGGAGAAGGAGCAAATGCTTATGGAATCCTTGGATAGAATAAAGTCCGTTGAGTTTGACCTTGAGAAGACAAAGAGGGTATTTGTCTATGCTTTCATCACTCATCTATGTCTTTTGATTAAGTTTGATCTGATGGTGTGGTTTTTAAAATTTGTGTTTTTTCTGCAGGTGCTGCACGCTGCAGTGATGAAGCAGCTTGAAATTAATGATTTACTGGAGAACATGCGGCAGCCGAAGTGTCGAGTAAGTTGATCATTGTATGATATTTAAAATATTTATATATAAGGAACATACTTGAAGAGGATGATAAACTTCATGAATCTGTAATTAAACTAAAGAAGTAAACTTGAAGAGAAGGATAAACCTCAAAAGTAGAAACTTTTTTGACTGCTGTGATCATCTGTATTAATCTTTTAACCGCATATGTTTGTAAACCTATAAGTTGCAACATCATCAGTTAAGAACAAGTTGCTTGTGTGAGCTTTAAATTGGGCAAGCTTGTCATTAACTTTTGCATTATTATTCTTGCAGCAGAGAAGGCTATTCTGTTGAATCATCATTTTTAGTTACAGAAGTCCTTTAAAAGTCAGATATCAGGCAAGCTCGCACAGTCGAGCGTTGATCGGACCCAAGGAAGCCCGAGGCGAGGCATTCAAGTCTAA
Protein sequence

>MS.gene63546.t1

MNASSKFTHSLKKRGKRKIDYRVPPVAIEDVRDAREETAVLELRQRLVERGSLPSRHDDYHTLLRFLKARDFNIEKTIEMWEEMLTWRKEYGTDTILEDFEFEELEEVLQYYPQGYHGVDKEGRPVYIERLGKAHPSRLMHITTIDRYLKYHVQEFERALQEKFPACSIAAKRQIFSTTTILDVQGLGMKNFSRTAANLLASMAKIDSCYYPETLHQMYIVNAGTGFRKMLWPAAQKFIDPQTIAKIQILDSKSLYKLQEVIDSSQLPDFLGGSCKCPGEGGCLRSNKGPWNDPDIMKLSGNAEATFVRQITRASNEQNNFDSFQLHSLKGRCSDSSAESGSDFNDYSSPTRQRRCSYPRLAPVCEEVRVPDVNGYYSCDDSALSAQNVIENDHHRLTREQSLQTNDMENIACRTNSEGTAVSNLLSIIKEKLERINFLYVPQVLTSFMERLAAFICSIRFEFWRTQNIVHPSNSTERVIINHSAAIESTSERDYILPCEQRLQRLEKVFNELNNKPDGMPLEKEQMLMESLDRIKSVEFDLEKTKRVLHAAVMKQLEINDLLENMRQPKCRISGKLAQSSVDRTQGSPRRGIQV