Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64692.t1 | XP_003612062.1 | 93.6 | 125 | 8 | 0 | 1 | 125 | 1 | 125 | 3.90E-59 | 237.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64692.t1 | G7KF00 | 93.6 | 125 | 8 | 0 | 1 | 125 | 1 | 125 | 2.8e-59 | 237.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056530 | MS.gene64692 | 0.809363 | 1.96E-50 | -1.69E-46 |
| MS.gene059514 | MS.gene64692 | 0.802562 | 5.29E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64692.t1 | MTR_5g020860 | 93.600 | 125 | 8 | 0 | 1 | 125 | 1 | 125 | 2.39e-84 | 242 |
| MS.gene64692.t1 | MTR_8g077145 | 62.992 | 127 | 41 | 4 | 1 | 125 | 1 | 123 | 2.02e-49 | 154 |
| MS.gene64692.t1 | MTR_7g026910 | 71.429 | 91 | 5 | 1 | 1 | 91 | 1 | 70 | 6.27e-37 | 120 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64692.t1 | AT3G50900 | 40.741 | 135 | 49 | 5 | 1 | 125 | 1 | 114 | 1.46e-21 | 83.2 |
| MS.gene64692.t1 | AT2G18210 | 44.898 | 98 | 42 | 4 | 33 | 125 | 34 | 124 | 5.36e-19 | 77.0 |
| MS.gene64692.t1 | AT4G36500 | 43.011 | 93 | 49 | 1 | 33 | 125 | 34 | 122 | 6.77e-16 | 68.9 |
| MS.gene64692.t1 | AT5G66490 | 33.835 | 133 | 56 | 5 | 1 | 125 | 1 | 109 | 6.73e-13 | 60.8 |
Find 32 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAATTTGGTGGAAATAA+TGG | 0.233549 | 5.2:+12106827 | MS.gene64692:CDS |
| ATCAAATACAAAGGTAAATA+TGG | 0.271944 | 5.2:+12106901 | MS.gene64692:CDS |
| CACTACTTGCTTTCTTTGTT+GGG | 0.272849 | 5.2:-12106964 | None:intergenic |
| GAGAAATTAGATAAAGAATT+TGG | 0.286753 | 5.2:+12106815 | MS.gene64692:CDS |
| TCAACATATCTTGCAAATTC+AGG | 0.290589 | 5.2:-12107064 | None:intergenic |
| ATATTTACCTTTGTATTTGA+TGG | 0.294161 | 5.2:-12106899 | None:intergenic |
| ATGGCCTCTTCTAGTACTAT+TGG | 0.297989 | 5.2:-12106783 | None:intergenic |
| TCACTACTTGCTTTCTTTGT+TGG | 0.309163 | 5.2:-12106965 | None:intergenic |
| TACAAAGGTAAATATGGTTA+TGG | 0.309718 | 5.2:+12106907 | MS.gene64692:CDS |
| AAGAATTTGGTGGAAATAAT+GGG | 0.329371 | 5.2:+12106828 | MS.gene64692:CDS |
| ATGGTTATGGGTTCAACCTT+AGG | 0.342083 | 5.2:+12106920 | MS.gene64692:CDS |
| GAAAGCAAGTAGTGATCATA+AGG | 0.388968 | 5.2:+12106973 | MS.gene64692:CDS |
| TGGTTATGGGTTCAACCTTA+GGG | 0.404531 | 5.2:+12106921 | MS.gene64692:CDS |
| ACAAAGGTAAATATGGTTAT+GGG | 0.413220 | 5.2:+12106908 | MS.gene64692:CDS |
| AGGAGCACAAGTGTTCCTTC+AGG | 0.428206 | 5.2:+12106875 | MS.gene64692:CDS |
| CTAATTTCTCATACTTCTCA+TGG | 0.429115 | 5.2:-12106802 | None:intergenic |
| AAGATATGTTGAGTATTTGA+AGG | 0.455536 | 5.2:+12107075 | MS.gene64692:CDS |
| TATGTTGAGTATTTGAAGGA+AGG | 0.471592 | 5.2:+12107079 | MS.gene64692:CDS |
| TTGTAGATTAATATCCCCTA+AGG | 0.515423 | 5.2:-12106936 | None:intergenic |
| GGAGCACAAGTGTTCCTTCA+GGG | 0.516731 | 5.2:+12106876 | MS.gene64692:CDS |
| TTTGTATTTGATGGCCCTGA+AGG | 0.555468 | 5.2:-12106890 | None:intergenic |
| GTTGAGTATTTGAAGGAAGG+AGG | 0.555632 | 5.2:+12107082 | MS.gene64692:CDS |
| TTTGAAGGAAGGAGGCATGT+GGG | 0.559503 | 5.2:+12107090 | MS.gene64692:CDS |
| TATCATTTATAATACATCAC+AGG | 0.563417 | 5.2:-12107130 | None:intergenic |
| AAATTAGATAAAGAATTTGG+TGG | 0.563779 | 5.2:+12106818 | MS.gene64692:CDS |
| ATTTGAAGGAAGGAGGCATG+TGG | 0.565436 | 5.2:+12107089 | MS.gene64692:CDS |
| AAATCCAATAGTACTAGAAG+AGG | 0.567361 | 5.2:+12106779 | MS.gene64692:CDS |
| CAAATGAAGAGTACTTGAAG+AGG | 0.569869 | 5.2:+12106855 | MS.gene64692:CDS |
| AAATCAAAGAAATTGAGAAG+TGG | 0.589565 | 5.2:-12107013 | None:intergenic |
| AGTACTATTGGATTTAAACA+TGG | 0.604551 | 5.2:-12106771 | None:intergenic |
| TTCAGGGCCATCAAATACAA+AGG | 0.612946 | 5.2:+12106892 | MS.gene64692:CDS |
| GGTTATGGGTTCAACCTTAG+GGG | 0.624193 | 5.2:+12106922 | MS.gene64692:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTAGATAAAGAATTTGG+TGG | + | chr5.2:12106818-12106837 | MS.gene64692:CDS | 20.0% |
| !! | ATATTTACCTTTGTATTTGA+TGG | - | chr5.2:12106902-12106921 | None:intergenic | 20.0% |
| !! | ATCAAATACAAAGGTAAATA+TGG | + | chr5.2:12106901-12106920 | MS.gene64692:CDS | 20.0% |
| !! | GAGAAATTAGATAAAGAATT+TGG | + | chr5.2:12106815-12106834 | MS.gene64692:CDS | 20.0% |
| !!! | TTCTCAATTTCTTTGATTTT+CGG | + | chr5.2:12107017-12107036 | MS.gene64692:CDS | 20.0% |
| ! | AAATCAAAGAAATTGAGAAG+TGG | - | chr5.2:12107016-12107035 | None:intergenic | 25.0% |
| ! | AAGATATGTTGAGTATTTGA+AGG | + | chr5.2:12107075-12107094 | MS.gene64692:CDS | 25.0% |
| ! | ACAAAGGTAAATATGGTTAT+GGG | + | chr5.2:12106908-12106927 | MS.gene64692:CDS | 25.0% |
| ! | TACAAAGGTAAATATGGTTA+TGG | + | chr5.2:12106907-12106926 | MS.gene64692:CDS | 25.0% |
| !! | AAAGAATTTGGTGGAAATAA+TGG | + | chr5.2:12106827-12106846 | MS.gene64692:CDS | 25.0% |
| !! | AAGAATTTGGTGGAAATAAT+GGG | + | chr5.2:12106828-12106847 | MS.gene64692:CDS | 25.0% |
| AAATCCAATAGTACTAGAAG+AGG | + | chr5.2:12106779-12106798 | MS.gene64692:CDS | 30.0% | |
| CTAATTTCTCATACTTCTCA+TGG | - | chr5.2:12106805-12106824 | None:intergenic | 30.0% | |
| TATGTTGAGTATTTGAAGGA+AGG | + | chr5.2:12107079-12107098 | MS.gene64692:CDS | 30.0% | |
| TCAACATATCTTGCAAATTC+AGG | - | chr5.2:12107067-12107086 | None:intergenic | 30.0% | |
| TTGTAGATTAATATCCCCTA+AGG | - | chr5.2:12106939-12106958 | None:intergenic | 30.0% | |
| CACTACTTGCTTTCTTTGTT+GGG | - | chr5.2:12106967-12106986 | None:intergenic | 35.0% | |
| GAAAGCAAGTAGTGATCATA+AGG | + | chr5.2:12106973-12106992 | MS.gene64692:CDS | 35.0% | |
| TCACTACTTGCTTTCTTTGT+TGG | - | chr5.2:12106968-12106987 | None:intergenic | 35.0% | |
| !! | CAAATGAAGAGTACTTGAAG+AGG | + | chr5.2:12106855-12106874 | MS.gene64692:CDS | 35.0% |
| ATGGCCTCTTCTAGTACTAT+TGG | - | chr5.2:12106786-12106805 | None:intergenic | 40.0% | |
| ATGGTTATGGGTTCAACCTT+AGG | + | chr5.2:12106920-12106939 | MS.gene64692:CDS | 40.0% | |
| GTTGAGTATTTGAAGGAAGG+AGG | + | chr5.2:12107082-12107101 | MS.gene64692:CDS | 40.0% | |
| TGGTTATGGGTTCAACCTTA+GGG | + | chr5.2:12106921-12106940 | MS.gene64692:CDS | 40.0% | |
| TTCAGGGCCATCAAATACAA+AGG | + | chr5.2:12106892-12106911 | MS.gene64692:CDS | 40.0% | |
| ! | TTTGTATTTGATGGCCCTGA+AGG | - | chr5.2:12106893-12106912 | None:intergenic | 40.0% |
| GGTTATGGGTTCAACCTTAG+GGG | + | chr5.2:12106922-12106941 | MS.gene64692:CDS | 45.0% | |
| ! | ATTTGAAGGAAGGAGGCATG+TGG | + | chr5.2:12107089-12107108 | MS.gene64692:CDS | 45.0% |
| ! | TTTGAAGGAAGGAGGCATGT+GGG | + | chr5.2:12107090-12107109 | MS.gene64692:CDS | 45.0% |
| GGAGCACAAGTGTTCCTTCA+GGG | + | chr5.2:12106876-12106895 | MS.gene64692:CDS | 50.0% | |
| ! | AGGAGCACAAGTGTTCCTTC+AGG | + | chr5.2:12106875-12106894 | MS.gene64692:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 12106773 | 12107150 | 12106773 | ID=MS.gene64692 |
| chr5.2 | mRNA | 12106773 | 12107150 | 12106773 | ID=MS.gene64692.t1;Parent=MS.gene64692 |
| chr5.2 | exon | 12106773 | 12107150 | 12106773 | ID=MS.gene64692.t1.exon1;Parent=MS.gene64692.t1 |
| chr5.2 | CDS | 12106773 | 12107150 | 12106773 | ID=cds.MS.gene64692.t1;Parent=MS.gene64692.t1 |
| Gene Sequence |
| Protein sequence |
