Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64933.t1 | RHN40757.1 | 81.3 | 155 | 28 | 1 | 1 | 155 | 157 | 310 | 4.70E-46 | 194.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64933.t1 | Q9LDD1 | 44.5 | 155 | 72 | 3 | 1 | 154 | 192 | 333 | 2.7e-26 | 119.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64933.t1 | A0A396GPW6 | 81.3 | 155 | 28 | 1 | 1 | 155 | 157 | 310 | 3.4e-46 | 194.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene64933 | 0.803111 | 4.07E-49 | -1.69E-46 |
| MS.gene049350 | MS.gene64933 | 0.806302 | 8.79E-50 | -1.69E-46 |
| MS.gene052463 | MS.gene64933 | 0.804875 | 1.75E-49 | -1.69E-46 |
| MS.gene053677 | MS.gene64933 | 0.801827 | 7.49E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene64933 | 0.804059 | 2.59E-49 | -1.69E-46 |
| MS.gene060763 | MS.gene64933 | 0.802559 | 5.30E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64933.t1 | MTR_2g069350 | 47.059 | 170 | 70 | 4 | 1 | 154 | 200 | 365 | 5.79e-42 | 143 |
| MS.gene64933.t1 | MTR_2g028830 | 44.937 | 158 | 58 | 4 | 1 | 154 | 186 | 318 | 1.19e-35 | 125 |
| MS.gene64933.t1 | MTR_4g083540 | 40.523 | 153 | 64 | 3 | 2 | 154 | 167 | 292 | 5.92e-29 | 107 |
| MS.gene64933.t1 | MTR_5g013690 | 32.692 | 156 | 78 | 5 | 2 | 154 | 140 | 271 | 1.22e-14 | 69.3 |
| MS.gene64933.t1 | MTR_5g013690 | 32.692 | 156 | 78 | 5 | 2 | 154 | 203 | 334 | 2.59e-14 | 68.9 |
| MS.gene64933.t1 | MTR_1g019250 | 30.065 | 153 | 85 | 4 | 2 | 154 | 210 | 340 | 5.50e-12 | 62.4 |
| MS.gene64933.t1 | MTR_4g036485 | 30.405 | 148 | 64 | 5 | 5 | 150 | 97 | 207 | 7.45e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64933.t1 | AT3G12920 | 49.057 | 159 | 59 | 5 | 1 | 154 | 192 | 333 | 7.45e-40 | 137 |
| MS.gene64933.t1 | AT1G79110 | 45.062 | 162 | 66 | 3 | 1 | 154 | 185 | 331 | 6.40e-33 | 119 |
| MS.gene64933.t1 | AT1G79110 | 45.062 | 162 | 66 | 3 | 1 | 154 | 210 | 356 | 6.79e-33 | 119 |
| MS.gene64933.t1 | AT1G79110 | 45.062 | 162 | 66 | 3 | 1 | 154 | 207 | 353 | 7.32e-33 | 119 |
| MS.gene64933.t1 | AT5G45100 | 41.667 | 156 | 62 | 5 | 1 | 154 | 137 | 265 | 1.42e-28 | 106 |
| MS.gene64933.t1 | AT5G45100 | 41.667 | 156 | 62 | 5 | 1 | 154 | 164 | 292 | 1.71e-28 | 106 |
| MS.gene64933.t1 | AT4G19700 | 38.462 | 156 | 67 | 4 | 1 | 154 | 174 | 302 | 3.97e-26 | 100 |
| MS.gene64933.t1 | AT1G10650 | 31.788 | 151 | 84 | 3 | 2 | 152 | 149 | 280 | 7.47e-15 | 70.1 |
| MS.gene64933.t1 | AT1G10650 | 31.788 | 151 | 84 | 3 | 2 | 152 | 205 | 336 | 1.94e-14 | 69.3 |
| MS.gene64933.t1 | AT4G35070 | 30.921 | 152 | 78 | 5 | 2 | 152 | 82 | 207 | 1.72e-12 | 62.8 |
| MS.gene64933.t1 | AT4G35070 | 31.333 | 150 | 76 | 5 | 2 | 150 | 137 | 260 | 4.87e-12 | 62.4 |
Find 31 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGTTCAATGTGTGGGGCTA+TGG | 0.402214 | 8.2:-50140444 | MS.gene64933:CDS |
| ACAAATCCTTATGCAACAAA+TGG | 0.418067 | 8.2:-50140714 | MS.gene64933:CDS |
| TGATTTCGCCGTTGCGGTGG+CGG | 0.422427 | 8.2:-50140648 | MS.gene64933:CDS |
| ATATTCATCATTACTACAAC+AGG | 0.424115 | 8.2:+50140613 | None:intergenic |
| AGTAATGACGGCGCAGGATA+AGG | 0.449129 | 8.2:-50140573 | MS.gene64933:CDS |
| TATGCATGGAGAATCAAATC+TGG | 0.461576 | 8.2:-50140793 | MS.gene64933:CDS |
| TATGTTTGTGTTCAATGTGT+GGG | 0.464104 | 8.2:-50140451 | MS.gene64933:CDS |
| ATGAATATTGTGGGTGGCGT+AGG | 0.488245 | 8.2:-50140598 | MS.gene64933:CDS |
| CACAAACATAAGTGTCTACA+AGG | 0.505076 | 8.2:+50140464 | None:intergenic |
| TTTCGCCGTTGCGGTGGCGG+AGG | 0.517029 | 8.2:-50140645 | MS.gene64933:CDS |
| GATTTAACAGAATCACATAT+AGG | 0.518774 | 8.2:+50140410 | None:intergenic |
| AAGGATGAAGGTACTAGTAG+TGG | 0.520250 | 8.2:-50140554 | MS.gene64933:CDS |
| AAAAGTGAAAACACTATGCA+TGG | 0.524997 | 8.2:-50140807 | MS.gene64933:CDS |
| TGTAGTAATGATGAATATTG+TGG | 0.529768 | 8.2:-50140608 | MS.gene64933:CDS |
| AGCATCCTCCGCCACCGCAA+CGG | 0.550649 | 8.2:+50140640 | None:intergenic |
| TTATGTTTGTGTTCAATGTG+TGG | 0.554213 | 8.2:-50140452 | MS.gene64933:CDS |
| CGGTGATTTCGCCGTTGCGG+TGG | 0.555761 | 8.2:-50140651 | MS.gene64933:CDS |
| ATAGATTATGCAGAAACTGT+GGG | 0.556378 | 8.2:-50140514 | MS.gene64933:CDS |
| GTAGTAATGATGAATATTGT+GGG | 0.574573 | 8.2:-50140607 | MS.gene64933:CDS |
| AATAGATTATGCAGAAACTG+TGG | 0.580193 | 8.2:-50140515 | MS.gene64933:CDS |
| TTATGCAGAAACTGTGGGGT+AGG | 0.580824 | 8.2:-50140509 | MS.gene64933:CDS |
| ATGTTTGTGTTCAATGTGTG+GGG | 0.595264 | 8.2:-50140450 | MS.gene64933:CDS |
| ACGGCGCAGGATAAGGATGA+AGG | 0.596413 | 8.2:-50140566 | MS.gene64933:CDS |
| CGGCGGTGATTTCGCCGTTG+CGG | 0.598700 | 8.2:-50140654 | MS.gene64933:CDS |
| TGAATATTGTGGGTGGCGTA+GGG | 0.602718 | 8.2:-50140597 | MS.gene64933:CDS |
| TAGGGTAGTAATGACGGCGC+AGG | 0.626421 | 8.2:-50140579 | MS.gene64933:CDS |
| GTGGCGTAGGGTAGTAATGA+CGG | 0.629194 | 8.2:-50140585 | MS.gene64933:CDS |
| AACGGCGACGGTGAATATAG+CGG | 0.693191 | 8.2:-50140674 | MS.gene64933:CDS |
| TAGATTATGCAGAAACTGTG+GGG | 0.713432 | 8.2:-50140513 | MS.gene64933:CDS |
| GGCGACGGTGAATATAGCGG+CGG | 0.715872 | 8.2:-50140671 | MS.gene64933:CDS |
| GTAATGATGAATATTGTGGG+TGG | 0.732674 | 8.2:-50140604 | MS.gene64933:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATATTCATCATTACTACAAC+AGG | + | chr8.2:50140599-50140618 | None:intergenic | 25.0% |
| ! | GATTTAACAGAATCACATAT+AGG | + | chr8.2:50140802-50140821 | None:intergenic | 25.0% |
| ! | GTAGTAATGATGAATATTGT+GGG | - | chr8.2:50140602-50140621 | MS.gene64933:CDS | 25.0% |
| ! | TGTAGTAATGATGAATATTG+TGG | - | chr8.2:50140601-50140620 | MS.gene64933:CDS | 25.0% |
| AAAAGTGAAAACACTATGCA+TGG | - | chr8.2:50140402-50140421 | MS.gene64933:CDS | 30.0% | |
| AATAGATTATGCAGAAACTG+TGG | - | chr8.2:50140694-50140713 | MS.gene64933:CDS | 30.0% | |
| ACAAATCCTTATGCAACAAA+TGG | - | chr8.2:50140495-50140514 | MS.gene64933:CDS | 30.0% | |
| ATAGATTATGCAGAAACTGT+GGG | - | chr8.2:50140695-50140714 | MS.gene64933:CDS | 30.0% | |
| GAAAACAACAACAACAACAA+CGG | - | chr8.2:50140517-50140536 | MS.gene64933:CDS | 30.0% | |
| TATGTTTGTGTTCAATGTGT+GGG | - | chr8.2:50140758-50140777 | MS.gene64933:CDS | 30.0% | |
| TTATGTTTGTGTTCAATGTG+TGG | - | chr8.2:50140757-50140776 | MS.gene64933:CDS | 30.0% | |
| !! | TGTTTTCCATTTGTTGCATA+AGG | + | chr8.2:50140504-50140523 | None:intergenic | 30.0% |
| ATGTTTGTGTTCAATGTGTG+GGG | - | chr8.2:50140759-50140778 | MS.gene64933:CDS | 35.0% | |
| CACAAACATAAGTGTCTACA+AGG | + | chr8.2:50140748-50140767 | None:intergenic | 35.0% | |
| GTAATGATGAATATTGTGGG+TGG | - | chr8.2:50140605-50140624 | MS.gene64933:CDS | 35.0% | |
| TAGATTATGCAGAAACTGTG+GGG | - | chr8.2:50140696-50140715 | MS.gene64933:CDS | 35.0% | |
| TATGCATGGAGAATCAAATC+TGG | - | chr8.2:50140416-50140435 | MS.gene64933:CDS | 35.0% | |
| !! | AAGGATGAAGGTACTAGTAG+TGG | - | chr8.2:50140655-50140674 | MS.gene64933:CDS | 40.0% |
| AACAACAACAACAACGGCGA+CGG | - | chr8.2:50140523-50140542 | MS.gene64933:CDS | 45.0% | |
| ATGAATATTGTGGGTGGCGT+AGG | - | chr8.2:50140611-50140630 | MS.gene64933:CDS | 45.0% | |
| TGAATATTGTGGGTGGCGTA+GGG | - | chr8.2:50140612-50140631 | MS.gene64933:CDS | 45.0% | |
| TTATGCAGAAACTGTGGGGT+AGG | - | chr8.2:50140700-50140719 | MS.gene64933:CDS | 45.0% | |
| AACGGCGACGGTGAATATAG+CGG | - | chr8.2:50140535-50140554 | MS.gene64933:CDS | 50.0% | |
| AGTAATGACGGCGCAGGATA+AGG | - | chr8.2:50140636-50140655 | MS.gene64933:CDS | 50.0% | |
| GTGGCGTAGGGTAGTAATGA+CGG | - | chr8.2:50140624-50140643 | MS.gene64933:CDS | 50.0% | |
| GTGTTCAATGTGTGGGGCTA+TGG | - | chr8.2:50140765-50140784 | MS.gene64933:CDS | 50.0% | |
| ACGGCGCAGGATAAGGATGA+AGG | - | chr8.2:50140643-50140662 | MS.gene64933:CDS | 55.0% | |
| TAGGGTAGTAATGACGGCGC+AGG | - | chr8.2:50140630-50140649 | MS.gene64933:CDS | 55.0% | |
| GGCGACGGTGAATATAGCGG+CGG | - | chr8.2:50140538-50140557 | MS.gene64933:CDS | 60.0% | |
| TGATTTCGCCGTTGCGGTGG+CGG | - | chr8.2:50140561-50140580 | MS.gene64933:CDS | 60.0% | |
| AGCATCCTCCGCCACCGCAA+CGG | + | chr8.2:50140572-50140591 | None:intergenic | 65.0% | |
| CGGCGGTGATTTCGCCGTTG+CGG | - | chr8.2:50140555-50140574 | MS.gene64933:CDS | 65.0% | |
| CGGTGATTTCGCCGTTGCGG+TGG | - | chr8.2:50140558-50140577 | MS.gene64933:CDS | 65.0% | |
| ! | TTTCGCCGTTGCGGTGGCGG+AGG | - | chr8.2:50140564-50140583 | MS.gene64933:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 50140382 | 50140849 | 50140382 | ID=MS.gene64933 |
| chr8.2 | mRNA | 50140382 | 50140849 | 50140382 | ID=MS.gene64933.t1;Parent=MS.gene64933 |
| chr8.2 | exon | 50140382 | 50140849 | 50140382 | ID=MS.gene64933.t1.exon1;Parent=MS.gene64933.t1 |
| chr8.2 | CDS | 50140382 | 50140849 | 50140382 | ID=cds.MS.gene64933.t1;Parent=MS.gene64933.t1 |
| Gene Sequence |
| Protein sequence |
