AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65321

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65321.t1 MTR_5g024070 93.569 311 20 0 1 311 20 330 0.0 597
MS.gene65321.t1 MTR_3g435550 36.296 135 78 2 162 295 38 165 9.88e-22 90.9
MS.gene65321.t1 MTR_3g091700 37.600 125 60 2 171 295 148 254 4.20e-19 85.5
MS.gene65321.t1 MTR_3g104830 48.276 87 40 2 180 264 393 476 1.79e-13 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65321.t1 AT4G35590 50.485 309 125 8 1 294 9 304 2.89e-87 264
MS.gene65321.t1 AT4G35590 50.323 310 124 9 1 294 59 354 1.93e-86 264
MS.gene65321.t1 AT1G74480 45.217 115 58 2 180 292 131 242 3.37e-22 94.7
MS.gene65321.t1 AT1G18790 43.802 121 62 3 175 293 120 236 4.17e-22 94.0
MS.gene65321.t1 AT5G66990 41.818 110 60 2 187 295 164 270 7.43e-20 87.8
MS.gene65321.t1 AT5G53040 41.739 115 54 1 181 295 147 248 1.53e-19 86.7
MS.gene65321.t1 AT5G16100 50.000 64 32 0 174 237 346 409 1.83e-13 70.9

Find 56 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGATTACTTTATCAGATT+TGG 0.267461 5.2:-14974631 MS.gene65321:CDS
AAAAGGAAGTGCAGAGAATT+TGG 0.277802 5.2:-14974543 MS.gene65321:CDS
TGAAAGCTACTCCAATTCTT+AGG 0.296460 5.2:-14976556 MS.gene65321:CDS
CAGTCTCATTCATGATCTTC+AGG 0.329225 5.2:-14974481 MS.gene65321:intron
TTGAGATCATTAAGCAGGTT+TGG 0.348731 5.2:+14975148 None:intergenic
TTTCAGGAATCCAAAGCTTC+AGG 0.353036 5.2:-14975176 MS.gene65321:intron
TTCTTTGACGCTTCAGCAAT+TGG 0.353848 5.2:+14974591 None:intergenic
TTTGGAATGGCCTGAAGCTT+TGG 0.353863 5.2:+14975166 None:intergenic
AGAAGACGAGTCTGAGTTAC+TGG 0.356355 5.2:-14974975 MS.gene65321:CDS
GAGATCTGCAAGGGAAGAAT+TGG 0.360744 5.2:-14975077 MS.gene65321:CDS
ATACCTTGTAACCAACAAAA+AGG 0.370413 5.2:+14976421 None:intergenic
GATTTCAACAACCTAAGAAT+TGG 0.374799 5.2:+14976545 None:intergenic
GTATTCCTCGCTGGCCTCAT+AGG 0.379146 5.2:-14974521 MS.gene65321:CDS
AAGGGTATAAGAATGGAGTT+TGG 0.381031 5.2:-14976499 MS.gene65321:CDS
AGATGCTAATTGCTAGGTTT+AGG 0.384292 5.2:+14973716 None:intergenic
AGTCTTGCCTGTGCTTAATC+AGG 0.396954 5.2:-14975020 MS.gene65321:CDS
TCTTCCCTTGCAGATCTCTC+TGG 0.397077 5.2:+14975082 None:intergenic
CATAGAGAAGAGGCTCTTCA+TGG 0.402183 5.2:-14973834 MS.gene65321:CDS
TTTCTCCTCCATCACATTCA+CGG 0.441238 5.2:+14976630 None:intergenic
CGTCAAAGAATCTAAATGTT+GGG 0.449206 5.2:-14974578 MS.gene65321:CDS
TTGCCTTCTTATCACTGCCA+TGG 0.457172 5.2:+14973883 None:intergenic
AAAGATGTTGGAAAGTGAAA+AGG 0.459498 5.2:-14973861 MS.gene65321:CDS
TTGGGCTTACTGTTCTCAAA+AGG 0.466425 5.2:-14974560 MS.gene65321:CDS
AACATCTTGTCTGAATCTCT+TGG 0.478887 5.2:+14973796 None:intergenic
TCAGACTCGTCTTCTTCAAA+AGG 0.484709 5.2:+14974983 None:intergenic
ACAGGCAAGACTATCTTTAG+TGG 0.485270 5.2:+14975031 None:intergenic
GAGAATTTGGTATTCCTCGC+TGG 0.487057 5.2:-14974530 MS.gene65321:CDS
TCATGTTGAGATCATTAAGC+AGG 0.496279 5.2:+14975143 None:intergenic
CAGCCATGGCAGTGATAAGA+AGG 0.499451 5.2:-14973886 MS.gene65321:CDS
GCAGGAAGAGGCAAAACATC+AGG 0.500010 5.2:-14973927 MS.gene65321:intron
ATCATTAAGCAGGTTTGGAA+TGG 0.504221 5.2:+14975153 None:intergenic
GATAAGAAGGCAAAAGATGT+TGG 0.505266 5.2:-14973873 MS.gene65321:CDS
GCAAGACTATCTTTAGTGGT+TGG 0.512102 5.2:+14975035 None:intergenic
TGAATGTGATGGAGGAGAAA+AGG 0.512445 5.2:-14976627 MS.gene65321:CDS
TCTCAACATGATATACAAGT+TGG 0.522938 5.2:-14975131 MS.gene65321:CDS
AATTGGCATATCAAAGTACT+TGG 0.526594 5.2:+14974608 None:intergenic
AATCTTTGCAACATGGTCAC+TGG 0.527182 5.2:+14974647 None:intergenic
TTGGAATGTGAGAAAATTGA+GGG 0.529395 5.2:+14976444 None:intergenic
ATACAAGTTGGAACCGAAGA+AGG 0.529415 5.2:-14975119 MS.gene65321:CDS
AGAGGCAAAACATCAGGAAA+GGG 0.535237 5.2:-14973921 MS.gene65321:CDS
ATCAGAAGATGCTAATTGCT+AGG 0.535738 5.2:+14973710 None:intergenic
GCGTCAAAGAATCTAAATGT+TGG 0.549395 5.2:-14974579 MS.gene65321:CDS
GTTGGAATGTGAGAAAATTG+AGG 0.552092 5.2:+14976443 None:intergenic
AAGAGGCAAAACATCAGGAA+AGG 0.561722 5.2:-14973922 MS.gene65321:CDS
AAAAGGAAAACATAGAGAAG+AGG 0.578123 5.2:-14973844 MS.gene65321:CDS
GGAGGATAAAGATGCAGCCA+TGG 0.600209 5.2:-14973900 MS.gene65321:CDS
AGTGATTTGATCTTCCTATG+AGG 0.604696 5.2:+14974507 None:intergenic
TTGAGATCCTGATTAAGCAC+AGG 0.605314 5.2:+14975013 None:intergenic
GGCAAAACATCAGGAAAGGG+AGG 0.624378 5.2:-14973918 MS.gene65321:CDS
CATGTGTACCGTGAATGTGA+TGG 0.635852 5.2:-14976638 MS.gene65321:CDS
TACACCAGAGAGATCTGCAA+GGG 0.646872 5.2:-14975086 MS.gene65321:CDS
ATAAAGTAATCTTTGCAACA+TGG 0.664575 5.2:+14974640 None:intergenic
ATCTTCCTATGAGGCCAGCG+AGG 0.669880 5.2:+14974516 None:intergenic
TCTGCAAGGGAAGAATTGGA+AGG 0.670458 5.2:-14975073 MS.gene65321:CDS
GTGTACCGTGAATGTGATGG+AGG 0.689782 5.2:-14976635 MS.gene65321:CDS
ATACACCAGAGAGATCTGCA+AGG 0.711933 5.2:-14975087 MS.gene65321:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATACACTTATTTTATTAAT+AGG - chr5.2:14975548-14975567 MS.gene65321:intron 10.0%
!! AACAATTCAAATTCAATAAA+AGG + chr5.2:14973990-14974009 None:intergenic 15.0%
!! ACAATTCAAATTCAATAAAA+GGG + chr5.2:14973989-14974008 None:intergenic 15.0%
!! ATTATATGATTATGTATTCT+GGG - chr5.2:14974017-14974036 MS.gene65321:intron 15.0%
!! CATACTAATAAATAAAAAAG+TGG + chr5.2:14975923-14975942 None:intergenic 15.0%
!!! TATTTTATTAATAGGAATCA+TGG - chr5.2:14975556-14975575 MS.gene65321:intron 15.0%
!! AAAGATTACTTTATCAGATT+TGG - chr5.2:14975740-14975759 MS.gene65321:intron 20.0%
!! AATACAAATTGATTAAAGCA+GGG + chr5.2:14974498-14974517 None:intergenic 20.0%
!! ACATTTATATATCATTCACA+GGG + chr5.2:14974572-14974591 None:intergenic 20.0%
!! ATGAAAATTTATAGTATCTG+AGG - chr5.2:14976108-14976127 MS.gene65321:intron 20.0%
!! GATTATATGATTATGTATTC+TGG - chr5.2:14974016-14974035 MS.gene65321:intron 20.0%
!! TAAAATAAGTGTATAAAGCT+AGG + chr5.2:14975543-14975562 None:intergenic 20.0%
!! TACATTTATATATCATTCAC+AGG + chr5.2:14974573-14974592 None:intergenic 20.0%
!! TATTTCAACCAAATACTATA+AGG + chr5.2:14976083-14976102 None:intergenic 20.0%
!! TTATAGACCAAATACTTTAT+AGG + chr5.2:14976336-14976355 None:intergenic 20.0%
!! TTGTAACTTAAACAAAATCA+CGG + chr5.2:14976234-14976253 None:intergenic 20.0%
!!! GAATATGTTTTTTGATTTCT+TGG - chr5.2:14975140-14975159 MS.gene65321:CDS 20.0%
!!! TATTGATTTCTTTATGTATG+TGG - chr5.2:14974757-14974776 MS.gene65321:intron 20.0%
!!! TTTTTTAGGAAAGAAAAAGA+GGG - chr5.2:14975698-14975717 MS.gene65321:intron 20.0%
! AGTTATCTAATCTAACAAAC+AGG + chr5.2:14974715-14974734 None:intergenic 25.0%
! ATAAAGTAATCTTTGCAACA+TGG + chr5.2:14975734-14975753 None:intergenic 25.0%
! ATTGCAAACCTTATAGTATT+TGG - chr5.2:14976072-14976091 MS.gene65321:intron 25.0%
! CAATACAAATTGATTAAAGC+AGG + chr5.2:14974499-14974518 None:intergenic 25.0%
! CTAAATATTAGTTCAAGAGT+TGG + chr5.2:14976022-14976041 None:intergenic 25.0%
! GACATAATTAACTCAACATT+AGG + chr5.2:14975632-14975651 None:intergenic 25.0%
! GTACAGAAAATATTTGTACA+AGG + chr5.2:14976151-14976170 None:intergenic 25.0%
! GTTGTCAATTAGTATATGTT+TGG - chr5.2:14976175-14976194 MS.gene65321:intron 25.0%
!! GTAAATCATAGAAGTTTTCT+TGG + chr5.2:14975997-14976016 None:intergenic 25.0%
!!! ATTTTTGAAGGGTATAAGAA+TGG - chr5.2:14973865-14973884 MS.gene65321:CDS 25.0%
!!! GTACCAATGTTTTTAAATAG+CGG - chr5.2:14974081-14974100 MS.gene65321:intron 25.0%
!!! GTTTTTTAGGAAAGAAAAAG+AGG - chr5.2:14975697-14975716 MS.gene65321:intron 25.0%
!!! TTTCTGAAGAGATTTTTGAA+GGG - chr5.2:14973854-14973873 MS.gene65321:CDS 25.0%
AAAAGGAAAACATAGAGAAG+AGG - chr5.2:14976527-14976546 MS.gene65321:CDS 30.0%
AAAATTGAAGAACATGACAG+AGG + chr5.2:14975178-14975197 None:intergenic 30.0%
AAAGATGTTGGAAAGTGAAA+AGG - chr5.2:14976510-14976529 MS.gene65321:CDS 30.0%
AGAGTTTGATAAAATCACAG+TGG - chr5.2:14976206-14976225 MS.gene65321:intron 30.0%
ATACCTTGTAACCAACAAAA+AGG + chr5.2:14973953-14973972 None:intergenic 30.0%
ATTAGGAAGAAGTTATGCAT+TGG + chr5.2:14975615-14975634 None:intergenic 30.0%
CGTCAAAGAATCTAAATGTT+GGG - chr5.2:14975793-14975812 MS.gene65321:intron 30.0%
GATTTCAACAACCTAAGAAT+TGG + chr5.2:14973829-14973848 None:intergenic 30.0%
GGAATAAAATGTTCAAAAGC+AGG + chr5.2:14975509-14975528 None:intergenic 30.0%
GTTCAAAAATCAGTTACCAA+TGG + chr5.2:14974783-14974802 None:intergenic 30.0%
TAAGGAGTTCACTTAAAGAT+TGG + chr5.2:14976405-14976424 None:intergenic 30.0%
TAGCCGCTATTTAAAAACAT+TGG + chr5.2:14974087-14974106 None:intergenic 30.0%
TCTCAACATGATATACAAGT+TGG - chr5.2:14975240-14975259 MS.gene65321:intron 30.0%
TTATACAAAACAGTGTATCG+TGG + chr5.2:14974163-14974182 None:intergenic 30.0%
TTGGAATGTGAGAAAATTGA+GGG + chr5.2:14973930-14973949 None:intergenic 30.0%
TTGGTGAAGTTAGAATTTGA+GGG - chr5.2:14975009-14975028 MS.gene65321:CDS 30.0%
TTGTGAAATAGCAACTATAG+CGG - chr5.2:14974187-14974206 MS.gene65321:intron 30.0%
TTTGGTGAAGTTAGAATTTG+AGG - chr5.2:14975008-14975027 MS.gene65321:CDS 30.0%
! AATTGGCATATCAAAGTACT+TGG + chr5.2:14975766-14975785 None:intergenic 30.0%
! ACTGAAGCCTATAAAGTATT+TGG - chr5.2:14976326-14976345 MS.gene65321:intron 30.0%
! TGTCATGTTCTTCAATTTTC+AGG - chr5.2:14975179-14975198 MS.gene65321:intron 30.0%
! TTATGTATTCTGGGTTTGTT+TGG - chr5.2:14974026-14974045 MS.gene65321:intron 30.0%
! TTTTTAAATAGCGGCTATAG+CGG - chr5.2:14974090-14974109 MS.gene65321:intron 30.0%
!! GTTGTTTTCATAAGCTATCT+TGG + chr5.2:14974350-14974369 None:intergenic 30.0%
!! GTTTCTGAAGAGATTTTTGA+AGG - chr5.2:14973853-14973872 MS.gene65321:CDS 30.0%
AACATCTTGTCTGAATCTCT+TGG + chr5.2:14976578-14976597 None:intergenic 35.0%
AAGTATGTTGTGGAACAGTT+AGG + chr5.2:14974989-14975008 None:intergenic 35.0%
ACTAAGTTGTTTATCCTGAC+AGG - chr5.2:14974443-14974462 MS.gene65321:intron 35.0%
AGGATGAAAATCAGACAACT+CGG + chr5.2:14974916-14974935 None:intergenic 35.0%
AGTATGTTGTGGAACAGTTA+GGG + chr5.2:14974988-14975007 None:intergenic 35.0%
AGTGATTTGATCTTCCTATG+AGG + chr5.2:14975867-14975886 None:intergenic 35.0%
ATCATTAAGCAGGTTTGGAA+TGG + chr5.2:14975221-14975240 None:intergenic 35.0%
CTAAGTTGTTTATCCTGACA+GGG - chr5.2:14974444-14974463 MS.gene65321:intron 35.0%
CTCGGATTTCAAATCGTATT+TGG + chr5.2:14974898-14974917 None:intergenic 35.0%
CTTGTAACCAACAAAAAGGT+TGG + chr5.2:14973949-14973968 None:intergenic 35.0%
GAAATGGTGCAGTAAATGTT+TGG - chr5.2:14974822-14974841 MS.gene65321:intron 35.0%
GAACAACGTGATAATTGACT+GGG + chr5.2:14975487-14975506 None:intergenic 35.0%
GAGTTCACTTAAAGATTGGT+AGG + chr5.2:14976401-14976420 None:intergenic 35.0%
GATAAGAAGGCAAAAGATGT+TGG - chr5.2:14976498-14976517 MS.gene65321:CDS 35.0%
GATTCCTTACAGTTACGAAA+TGG - chr5.2:14974806-14974825 MS.gene65321:intron 35.0%
GCGTCAAAGAATCTAAATGT+TGG - chr5.2:14975792-14975811 MS.gene65321:intron 35.0%
GTTGGAATGTGAGAAAATTG+AGG + chr5.2:14973931-14973950 None:intergenic 35.0%
TAAAGTCCCTACACAAGTAA+CGG - chr5.2:14974620-14974639 MS.gene65321:CDS 35.0%
TATAGGCTTCAGTGCATATT+TGG + chr5.2:14976319-14976338 None:intergenic 35.0%
TCATGTTGAGATCATTAAGC+AGG + chr5.2:14975231-14975250 None:intergenic 35.0%
TGAAAGCTACTCCAATTCTT+AGG - chr5.2:14973815-14973834 MS.gene65321:CDS 35.0%
TTCTTTATGTATGTGGCCAT+TGG - chr5.2:14974764-14974783 MS.gene65321:intron 35.0%
TTGAGATCATTAAGCAGGTT+TGG + chr5.2:14975226-14975245 None:intergenic 35.0%
TTGATAAAGTGAGTGTTTGC+AGG - chr5.2:14975675-14975694 MS.gene65321:intron 35.0%
TTTCGTAACTGTAAGGAATC+CGG + chr5.2:14974806-14974825 None:intergenic 35.0%
! AAAAGGAAGTGCAGAGAATT+TGG - chr5.2:14975828-14975847 MS.gene65321:intron 35.0%
! AAGGGTATAAGAATGGAGTT+TGG - chr5.2:14973872-14973891 MS.gene65321:CDS 35.0%
! ACTGTTCCACAACATACTTT+TGG - chr5.2:14974990-14975009 MS.gene65321:CDS 35.0%
! ACTTCACCAAAAGTATGTTG+TGG + chr5.2:14974999-14975018 None:intergenic 35.0%
! CATAGCGGAATTTGAACAAA+CGG - chr5.2:14974129-14974148 MS.gene65321:intron 35.0%
! GTATCTTTTCGTTCCTTCTT+CGG + chr5.2:14975268-14975287 None:intergenic 35.0%
! GTTATAGTGCTATTGCATAG+CGG - chr5.2:14974114-14974133 MS.gene65321:intron 35.0%
! TAGCTTTTGTGCCAAAATCA+CGG - chr5.2:14976258-14976277 MS.gene65321:intron 35.0%
! TATCTGAGGCTTCTTTAGAT+AGG - chr5.2:14976122-14976141 MS.gene65321:intron 35.0%
! TCACATTCCAACCTTTTTGT+TGG - chr5.2:14973939-14973958 MS.gene65321:intron 35.0%
!! ACAGATTTTGAAAGTTCAGC+AGG - chr5.2:14975427-14975446 MS.gene65321:intron 35.0%
!! CAACCTTTTTGTTGGTTACA+AGG - chr5.2:14973947-14973966 MS.gene65321:intron 35.0%
!! GACAAGATGTTTTCAAGAGA+AGG - chr5.2:14976587-14976606 MS.gene65321:CDS 35.0%
!! GAGTTTGTGTTTTCTGAGAA+TGG - chr5.2:14973778-14973797 MS.gene65321:CDS 35.0%
!! TGGTAACTGATTTTTGAACC+CGG - chr5.2:14974784-14974803 MS.gene65321:intron 35.0%
!!! TGAGTGTTTGCAGGTTTTTT+AGG - chr5.2:14975684-14975703 MS.gene65321:intron 35.0%
AAGAGGCAAAACATCAGGAA+AGG - chr5.2:14976449-14976468 MS.gene65321:CDS 40.0%
AATCTTTGCAACATGGTCAC+TGG + chr5.2:14975727-14975746 None:intergenic 40.0%
ACAGGCAAGACTATCTTTAG+TGG + chr5.2:14975343-14975362 None:intergenic 40.0%
AGAACATGACAGAGGAAGTT+AGG + chr5.2:14975170-14975189 None:intergenic 40.0%
AGAGGCAAAACATCAGGAAA+GGG - chr5.2:14976450-14976469 MS.gene65321:CDS 40.0%
CAGTCTCATTCATGATCTTC+AGG - chr5.2:14975890-14975909 MS.gene65321:intron 40.0%
GAAGTTAGAATTTGAGGGCA+TGG - chr5.2:14975014-14975033 MS.gene65321:CDS 40.0%
GCAAGACTATCTTTAGTGGT+TGG + chr5.2:14975339-14975358 None:intergenic 40.0%
GCGATGTTGTCATGTGTATA+AGG + chr5.2:14976423-14976442 None:intergenic 40.0%
GCTTCAGTGCATATTTGGAT+TGG + chr5.2:14976314-14976333 None:intergenic 40.0%
GGAACAACGTGATAATTGAC+TGG + chr5.2:14975488-14975507 None:intergenic 40.0%
GGTGTGTACTGTGTACAAAT+TGG - chr5.2:14974545-14974564 MS.gene65321:CDS 40.0%
GTGCAGTAAATGTTTGGCAT+TGG - chr5.2:14974828-14974847 MS.gene65321:intron 40.0%
TAAAGCTAGGACTGAGTTGT+TGG + chr5.2:14975530-14975549 None:intergenic 40.0%
TCAGACTCGTCTTCTTCAAA+AGG + chr5.2:14975391-14975410 None:intergenic 40.0%
TCATCCTACAGTTGCAATTG+CGG - chr5.2:14974929-14974948 MS.gene65321:intron 40.0%
TGATTAAAGCAGGGAAACCT+TGG + chr5.2:14974489-14974508 None:intergenic 40.0%
TGCACCATTTCGTAACTGTA+AGG + chr5.2:14974813-14974832 None:intergenic 40.0%
TTCGTAACTGTAAGGAATCC+GGG + chr5.2:14974805-14974824 None:intergenic 40.0%
TTCTTTGACGCTTCAGCAAT+TGG + chr5.2:14975783-14975802 None:intergenic 40.0%
TTGAGATCCTGATTAAGCAC+AGG + chr5.2:14975361-14975380 None:intergenic 40.0%
TTGGGCTTACTGTTCTCAAA+AGG - chr5.2:14975811-14975830 MS.gene65321:intron 40.0%
TTGTGGAACAGTTAGGGAAA+TGG + chr5.2:14974982-14975001 None:intergenic 40.0%
TTTCAGGAATCCAAAGCTTC+AGG - chr5.2:14975195-14975214 MS.gene65321:intron 40.0%
TTTCTCCTCCATCACATTCA+CGG + chr5.2:14973744-14973763 None:intergenic 40.0%
! ATACAAGTTGGAACCGAAGA+AGG - chr5.2:14975252-14975271 MS.gene65321:intron 40.0%
! GGAGCATTTGAATCTAAACC+TGG + chr5.2:14975466-14975485 None:intergenic 40.0%
! TGAATGTGATGGAGGAGAAA+AGG - chr5.2:14973744-14973763 MS.gene65321:CDS 40.0%
!!! GCAGGTTTTGAAAGTTTACC+AGG - chr5.2:14975445-14975464 MS.gene65321:intron 40.0%
AGAAGACGAGTCTGAGTTAC+TGG - chr5.2:14975396-14975415 MS.gene65321:intron 45.0%
AGTCTTGCCTGTGCTTAATC+AGG - chr5.2:14975351-14975370 MS.gene65321:intron 45.0%
ATACACCAGAGAGATCTGCA+AGG - chr5.2:14975284-14975303 MS.gene65321:intron 45.0%
CATAGAGAAGAGGCTCTTCA+TGG - chr5.2:14976537-14976556 MS.gene65321:CDS 45.0%
CATGTGTACCGTGAATGTGA+TGG - chr5.2:14973733-14973752 MS.gene65321:CDS 45.0%
GAGAATTTGGTATTCCTCGC+TGG - chr5.2:14975841-14975860 MS.gene65321:intron 45.0%
GAGATCTGCAAGGGAAGAAT+TGG - chr5.2:14975294-14975313 MS.gene65321:intron 45.0%
GATTCGCCGTTACTTGTGTA+GGG + chr5.2:14974629-14974648 None:intergenic 45.0%
TACACCAGAGAGATCTGCAA+GGG - chr5.2:14975285-14975304 MS.gene65321:intron 45.0%
TCTGCAAGGGAAGAATTGGA+AGG - chr5.2:14975298-14975317 MS.gene65321:intron 45.0%
TGATTCGCCGTTACTTGTGT+AGG + chr5.2:14974630-14974649 None:intergenic 45.0%
TTGCCTTCTTATCACTGCCA+TGG + chr5.2:14976491-14976510 None:intergenic 45.0%
TTGGTCAGGCCTAAAAGCTA+AGG + chr5.2:14974470-14974489 None:intergenic 45.0%
! CTTTTGTGCCAAAATCACGG+TGG - chr5.2:14976261-14976280 MS.gene65321:intron 45.0%
! TGCTGCAAATGTTGACTGCA+GGG - chr5.2:14974953-14974972 MS.gene65321:CDS 45.0%
! TTTGGAATGGCCTGAAGCTT+TGG + chr5.2:14975208-14975227 None:intergenic 45.0%
!! TTAGCTTTTAGGCCTGACCA+AGG - chr5.2:14974469-14974488 MS.gene65321:intron 45.0%
AAAGCAGGGAAACCTTGGTC+AGG + chr5.2:14974484-14974503 None:intergenic 50.0%
ACACATGACAACATCGCTGC+AGG - chr5.2:14976426-14976445 MS.gene65321:CDS 50.0%
CAGCACACAGAGCAGCAAAA+TGG - chr5.2:14974524-14974543 MS.gene65321:CDS 50.0%
CAGCCATGGCAGTGATAAGA+AGG - chr5.2:14976485-14976504 MS.gene65321:CDS 50.0%
CTAAAAGCTAAGGCCCTGTC+AGG + chr5.2:14974460-14974479 None:intergenic 50.0%
GCAGGAAGAGGCAAAACATC+AGG - chr5.2:14976444-14976463 MS.gene65321:CDS 50.0%
GGAGGATAAAGATGCAGCCA+TGG - chr5.2:14976471-14976490 MS.gene65321:CDS 50.0%
GGCAAAACATCAGGAAAGGG+AGG - chr5.2:14976453-14976472 MS.gene65321:CDS 50.0%
GTGTACCGTGAATGTGATGG+AGG - chr5.2:14973736-14973755 MS.gene65321:CDS 50.0%
TAGCAACTATAGCGGCGCTA+TGG - chr5.2:14974195-14974214 MS.gene65321:intron 50.0%
TCTTCCCTTGCAGATCTCTC+TGG + chr5.2:14975292-14975311 None:intergenic 50.0%
! CTGCTGCAAATGTTGACTGC+AGG - chr5.2:14974952-14974971 MS.gene65321:CDS 50.0%
! TTGAGGGCATGGTTCAGCAA+TGG - chr5.2:14975025-14975044 MS.gene65321:CDS 50.0%
!! CTGACAGGGCCTTAGCTTTT+AGG - chr5.2:14974458-14974477 MS.gene65321:intron 50.0%
ATCTTCCTATGAGGCCAGCG+AGG + chr5.2:14975858-14975877 None:intergenic 55.0%
CGTTGAGACTGCCCGAATCA+CGG + chr5.2:14976291-14976310 None:intergenic 55.0%
GACAACATCGCTGCAGGAAG+AGG - chr5.2:14976432-14976451 MS.gene65321:CDS 55.0%
GCAGCCGCAATTGCAACTGT+AGG + chr5.2:14974936-14974955 None:intergenic 55.0%
GCTATGGCAGTGTTGCATGG+CGG - chr5.2:14974211-14974230 MS.gene65321:intron 55.0%
GGGCATGGTTCAGCAATGGA+TGG - chr5.2:14975029-14975048 MS.gene65321:CDS 55.0%
! ACGGTGAGCCACCGTGATTT+TGG + chr5.2:14976272-14976291 None:intergenic 55.0%
! GTATTCCTCGCTGGCCTCAT+AGG - chr5.2:14975850-14975869 MS.gene65321:intron 55.0%
ACGGTGGCTCACCGTGATTC+GGG - chr5.2:14976277-14976296 MS.gene65321:intron 60.0%
CACGGTGGCTCACCGTGATT+CGG - chr5.2:14976276-14976295 MS.gene65321:intron 60.0%
GGCGCTATGGCAGTGTTGCA+TGG - chr5.2:14974208-14974227 MS.gene65321:intron 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 14973721 14976672 14973721 ID=MS.gene65321
chr5.2 mRNA 14973721 14976672 14973721 ID=MS.gene65321.t1;Parent=MS.gene65321
chr5.2 exon 14976425 14976672 14976425 ID=MS.gene65321.t1.exon1;Parent=MS.gene65321.t1
chr5.2 CDS 14976425 14976672 14976425 ID=cds.MS.gene65321.t1;Parent=MS.gene65321.t1
chr5.2 exon 14974945 14975192 14974945 ID=MS.gene65321.t1.exon2;Parent=MS.gene65321.t1
chr5.2 CDS 14974945 14975192 14974945 ID=cds.MS.gene65321.t1;Parent=MS.gene65321.t1
chr5.2 exon 14974482 14974696 14974482 ID=MS.gene65321.t1.exon3;Parent=MS.gene65321.t1
chr5.2 CDS 14974482 14974696 14974482 ID=cds.MS.gene65321.t1;Parent=MS.gene65321.t1
chr5.2 exon 14973721 14973945 14973721 ID=MS.gene65321.t1.exon4;Parent=MS.gene65321.t1
chr5.2 CDS 14973721 14973945 14973721 ID=cds.MS.gene65321.t1;Parent=MS.gene65321.t1
Gene Sequence

>MS.gene65321

ATGAGAAGCGTGCATGTGTACCGTGAATGTGATGGAGGAGAAAAGGAAGTTGAAAGAGAGTTTGTGTTTTCTGAGAATGGTTCTTATGTTGAAATGAAAGCTACTCCAATTCTTAGGTTGTTGAAATCTTTTGTTTCTGAAGAGATTTTTGAAGGGTATAAGAATGGAGTTTGGCTTTGTGTTTTTGCTTTTCATGCTTGTAATGTCCCTCAATTTTCTCACATTCCAACCTTTTTGTTGGTTACAAGGTATGTATATCTACTTACCCTTTTATTGAATTTGAATTGTTTCATTTGATTATATGATTATGTATTCTGGGTTTGTTTGGATTCACTTATTTGAACTTATAACTTATGACATGTACCAATGTTTTTAAATAGCGGCTATAGCGGTGTTATAGTGCTATTGCATAGCGGAATTTGAACAAACGGCTATTGTTCCACGATACACTGTTTTGTATAAAATGTTGTGAAATAGCAACTATAGCGGCGCTATGGCAGTGTTGCATGGCGGAATTTGAACAAAACGCTATTTTCTGTAATCTGCAATTGACAACATTGAAATGCACATAATTTGTTAACATTGAAATGTACATAATCTGTTTTCAGTTTATTGAAATAAGCTCGCCAAGATAGCTTATGAAAACAACTTATAACATATATGAAAATAATTCGACTTTAGTTCATCTTTTGTTATAAATATAGTTTTTACATAAGTGCTTAACTAAGTTGTTTATCCTGACAGGGCCTTAGCTTTTAGGCCTGACCAAGGTTTCCCTGCTTTAATCAATTTGTATTGTAAAACAGCACACAGAGCAGCAAAATGGTGTGTACTGTGTACAAATTGGACCCTGTGAATGATATATAAATGTATTTCAGTTCTCTTATGAGAATGAAAAATAAAGTCCCTACACAAGTAACGGCGAATCACTCTATAATAAACAGTTTCTTTTCATAAACATAAAGATTCAAATTCAGTTATAAGTAACAGTCCTGTTTGTTAGATTAGATAACTGTAGTCTGTACATGTGTTCAAGTATTGATTTCTTTATGTATGTGGCCATTGGTAACTGATTTTTGAACCCGGATTCCTTACAGTTACGAAATGGTGCAGTAAATGTTTGGCATTGGATGAAGATCAGATGACTTGTATTTTAACTTTATTGAATCAAATTCCAAATACGATTTGAAATCCGAGTTGTCTGATTTTCATCCTACAGTTGCAATTGCGGCTGCTGCAAATGTTGACTGCAGGGTATCCATTTCCCTAACTGTTCCACAACATACTTTTGGTGAAGTTAGAATTTGAGGGCATGGTTCAGCAATGGATGGCATAAAATTTAGAAAATATATCTCTCTGTAACTATTTTTAGTGCATATTTTAATCATAACAAACGTAACAAAATTAATGCTTTGACTTGAATATGTTTTTTGATTTCTTGGTTGTCCTAACTTCCTCTGTCATGTTCTTCAATTTTCAGGAATCCAAAGCTTCAGGCCATTCCAAACCTGCTTAATGATCTCAACATGATATACAAGTTGGAACCGAAGAAGGAACGAAAAGATACACCAGAGAGATCTGCAAGGGAAGAATTGGAAGGAAACAACAATGATTTCCAACCACTAAAGATAGTCTTGCCTGTGCTTAATCAGGATCTCAATTGTCTTCCTTTTGAAGAAGACGAGTCTGAGTTACTGGATGATGAGACAGATTTTGAAAGTTCAGCAGGTTTTGAAAGTTTACCAGGTTTAGATTCAAATGCTCCCAGTCAATTATCACGTTGTTCCTGCTTTTGAACATTTTATTCCAACAACTCAGTCCTAGCTTTATACACTTATTTTATTAATAGGAATCATGGAGATAAAAAAGTTGTAGTGCAATAAATATGTTGCCAATGCATAACTTCTTCCTAATGTTGAGTTAATTATGTCATGTTTTAACATTTTATATCTAATTGATAAAGTGAGTGTTTGCAGGTTTTTTAGGAAAGAAAAAGAGGGCAGCCAGTGACCATGTTGCAAAGATTACTTTATCAGATTTGGCCAAGTACTTTGATATGCCAATTGCTGAAGCGTCAAAGAATCTAAATGTTGGGCTTACTGTTCTCAAAAGGAAGTGCAGAGAATTTGGTATTCCTCGCTGGCCTCATAGGAAGATCAAATCACTCGACAGTCTCATTCATGATCTTCAGGTTCTACTCCACTTTTTTATTTATTAGTATGACTTTAAGAATCTCTGTTGAACTATATATATACATACATACATATCAATTTCCAAGAAAACTTCTATGATTTACAGCCAACTCTTGAACTAATATTTAGATATTCGAACATGACTGTCATTTTAATGTGATTGCAAACCTTATAGTATTTGGTTGAAATATAAACATGAAAATTTATAGTATCTGAGGCTTCTTTAGATAGGTAACCTTGTACAAATATTTTCTGTACTTTTGTTGTCAATTAGTATATGTTTGGATGAGCGAAGAGTTTGATAAAATCACAGTGGCACCGTGATTTTGTTTAAGTTACAAAGAGTAGCTTTTGTGCCAAAATCACGGTGGCTCACCGTGATTCGGGCAGTCTCAACGCCAATCCAAATATGCACTGAAGCCTATAAAGTATTTGGTCTATAAAATCTTTGTATTTCGCTTTATTTTCTTTTATGCTTCACTTTTCCTACCAATCTTTAAGTGAACTCCTTATACACATGACAACATCGCTGCAGGAAGAGGCAAAACATCAGGAAAGGGAGGATAAAGATGCAGCCATGGCAGTGATAAGAAGGCAAAAGATGTTGGAAAGTGAAAAGGAAAACATAGAGAAGAGGCTCTTCATGGACATTCAGATTGAGACCAAGAGATTCAGACAAGATGTTTTCAAGAGAAGGCACAGAGCTAGAGTTATTGAAAACAGAATTCTACAGTTGCAAGCACCTAAACCTAGCAATTAG
Protein sequence

>MS.gene65321.t1

MRSVHVYRECDGGEKEVEREFVFSENGSYVEMKATPILRLLKSFVSEEIFEGYKNGVWLCVFAFHACNVPQFSHIPTFLLVTRNPKLQAIPNLLNDLNMIYKLEPKKERKDTPERSAREELEGNNNDFQPLKIVLPVLNQDLNCLPFEEDESELLDDETDFESSAGFLGKKKRAASDHVAKITLSDLAKYFDMPIAEASKNLNVGLTVLKRKCREFGIPRWPHRKIKSLDSLIHDLQEEAKHQEREDKDAAMAVIRRQKMLESEKENIEKRLFMDIQIETKRFRQDVFKRRHRARVIENRILQLQAPKPSN