AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65401

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65401.t1 MTR_4g130820 64.793 747 235 11 1 739 1 727 0.0 951
MS.gene65401.t1 MTR_1g028370 49.732 746 326 12 14 744 5 716 0.0 715
MS.gene65401.t1 MTR_3g091080 50.270 740 321 9 14 739 4 710 0.0 713
MS.gene65401.t1 MTR_2g460590 52.199 682 293 6 82 744 24 691 0.0 708
MS.gene65401.t1 MTR_3g109030 52.246 668 295 7 89 739 61 721 0.0 677
MS.gene65401.t1 MTR_2g094860 49.517 725 327 14 20 738 10 701 0.0 677
MS.gene65401.t1 MTR_7g117310 48.916 738 333 10 17 739 19 727 0.0 666
MS.gene65401.t1 MTR_7g117310 48.780 738 334 10 17 739 7 715 0.0 666
MS.gene65401.t1 MTR_4g058730 55.150 602 257 7 69 666 25 617 0.0 665
MS.gene65401.t1 MTR_7g117310 51.740 661 287 7 92 739 20 661 0.0 664
MS.gene65401.t1 MTR_1g064240 51.420 669 293 13 89 742 59 710 0.0 656
MS.gene65401.t1 MTR_7g034605 51.156 649 294 10 100 738 43 678 0.0 655
MS.gene65401.t1 MTR_8g027755 50.617 648 302 7 97 737 81 717 0.0 645
MS.gene65401.t1 MTR_3g088875 47.661 684 317 12 90 743 20 692 0.0 630
MS.gene65401.t1 MTR_6g477760 40.956 586 310 15 101 666 123 692 1.08e-127 396
MS.gene65401.t1 MTR_6g075290 39.626 588 324 12 100 672 192 763 1.13e-120 380
MS.gene65401.t1 MTR_5g007630 38.047 594 324 15 101 678 172 737 4.25e-117 370
MS.gene65401.t1 MTR_6g075440 34.429 700 400 18 16 686 90 759 5.05e-117 370
MS.gene65401.t1 MTR_6g075440 34.543 689 395 18 16 681 90 745 6.79e-117 370
MS.gene65401.t1 MTR_6g075290 39.160 595 319 16 93 672 156 722 3.71e-110 351
MS.gene65401.t1 MTR_8g464570 35.777 573 348 10 103 664 64 627 8.53e-110 348
MS.gene65401.t1 MTR_6g075290 39.437 568 311 13 120 672 12 561 2.43e-109 344
MS.gene65401.t1 MTR_7g012260 36.333 589 354 9 86 666 102 677 5.60e-109 347
MS.gene65401.t1 MTR_2g081110 34.835 577 351 11 99 664 61 623 1.65e-105 337
MS.gene65401.t1 MTR_6g075410 34.932 584 344 14 100 667 97 660 2.28e-96 313
MS.gene65401.t1 MTR_6g075390 35.596 545 312 13 100 627 114 636 2.04e-92 303
MS.gene65401.t1 MTR_6g076060 36.712 444 249 11 232 660 29 455 2.76e-72 244
MS.gene65401.t1 MTR_6g075320 41.176 119 70 0 100 218 148 266 4.94e-24 103
MS.gene65401.t1 MTR_2g094810 44.954 109 60 0 180 288 12 120 5.22e-24 98.2
MS.gene65401.t1 MTR_4g113530 25.366 205 150 2 379 582 239 441 5.01e-17 86.3
MS.gene65401.t1 MTR_3g108320 25.616 203 148 2 380 582 243 442 2.16e-16 83.6
MS.gene65401.t1 MTR_3g108320 25.616 203 148 2 380 582 243 442 2.28e-16 83.6
MS.gene65401.t1 MTR_3g108320 25.616 203 148 2 380 582 243 442 3.43e-16 83.2
MS.gene65401.t1 MTR_2g006870 24.020 204 152 2 379 582 262 462 7.39e-15 79.0
MS.gene65401.t1 MTR_5g077770 24.194 186 138 2 374 559 277 459 1.47e-12 71.6
MS.gene65401.t1 MTR_8g046440 19.792 192 153 1 374 565 29 219 5.70e-11 65.1
MS.gene65401.t1 MTR_6g075460 41.250 80 45 2 599 676 45 124 7.36e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65401.t1 AT1G19780 68.519 756 215 10 6 744 2 751 0.0 1024
MS.gene65401.t1 AT1G15990 68.133 750 209 14 6 739 2 737 0.0 999
MS.gene65401.t1 AT2G23980 62.914 755 253 10 1 739 1 744 0.0 955
MS.gene65401.t1 AT2G23980 62.914 755 253 10 1 739 1 744 0.0 955
MS.gene65401.t1 AT2G23980 62.914 755 253 10 1 739 1 744 0.0 955
MS.gene65401.t1 AT2G23980 62.914 755 253 10 1 739 1 744 0.0 955
MS.gene65401.t1 AT2G23980 62.914 755 253 10 1 739 1 744 0.0 955
MS.gene65401.t1 AT4G30560 63.431 752 239 9 1 739 1 729 0.0 949
MS.gene65401.t1 AT4G30560 63.431 752 239 9 1 739 1 729 0.0 949
MS.gene65401.t1 AT5G57940 64.441 734 238 8 11 739 1 716 0.0 943
MS.gene65401.t1 AT5G57940 64.441 734 238 8 11 739 1 716 0.0 943
MS.gene65401.t1 AT2G23980 61.054 778 253 11 1 739 1 767 0.0 941
MS.gene65401.t1 AT2G23980 61.054 778 253 11 1 739 1 767 0.0 941
MS.gene65401.t1 AT2G23980 61.054 778 253 11 1 739 1 767 0.0 941
MS.gene65401.t1 AT2G23980 61.054 778 253 11 1 739 1 767 0.0 941
MS.gene65401.t1 AT5G57940 63.896 734 235 8 11 739 1 709 0.0 930
MS.gene65401.t1 AT2G23980 63.049 728 246 9 24 739 1 717 0.0 928
MS.gene65401.t1 AT4G30560 63.636 726 231 8 24 739 1 703 0.0 924
MS.gene65401.t1 AT4G30560 63.636 726 231 8 24 739 1 703 0.0 924
MS.gene65401.t1 AT4G30560 63.636 726 231 8 24 739 1 703 0.0 924
MS.gene65401.t1 AT1G19780 70.425 541 145 7 217 744 2 540 0.0 754
MS.gene65401.t1 AT1G01340 53.707 661 279 6 92 739 59 705 0.0 718
MS.gene65401.t1 AT1G01340 53.707 661 279 6 92 739 54 700 0.0 717
MS.gene65401.t1 AT4G01010 51.489 705 293 11 53 741 24 695 0.0 704
MS.gene65401.t1 AT4G01010 51.489 705 293 11 53 741 24 695 0.0 704
MS.gene65401.t1 AT5G53130 49.730 742 326 10 14 739 4 714 0.0 692
MS.gene65401.t1 AT2G28260 50.946 687 305 8 55 739 21 677 0.0 683
MS.gene65401.t1 AT2G24610 52.444 675 287 11 89 739 60 724 0.0 682
MS.gene65401.t1 AT2G24610 52.444 675 287 11 89 739 75 739 0.0 681
MS.gene65401.t1 AT2G28260 52.024 667 289 7 74 739 1 637 0.0 680
MS.gene65401.t1 AT4G30360 51.672 658 299 10 99 743 69 720 0.0 655
MS.gene65401.t1 AT2G46430 47.241 743 327 12 14 737 5 701 0.0 653
MS.gene65401.t1 AT2G46430 47.241 743 327 12 14 737 5 701 0.0 653
MS.gene65401.t1 AT5G14870 49.552 670 303 9 98 738 37 700 0.0 647
MS.gene65401.t1 AT3G48010 47.640 678 303 12 98 737 41 704 0.0 623
MS.gene65401.t1 AT2G46440 43.339 623 309 13 99 706 28 621 3.96e-162 483
MS.gene65401.t1 AT2G46450 38.473 655 342 16 99 735 28 639 1.38e-148 449
MS.gene65401.t1 AT2G46450 38.473 655 342 16 99 735 28 639 1.38e-148 449
MS.gene65401.t1 AT2G46450 37.557 655 325 17 99 735 15 603 1.39e-140 427
MS.gene65401.t1 AT2G46450 37.557 655 325 17 99 735 28 616 1.87e-140 427
MS.gene65401.t1 AT2G46440 47.097 465 208 10 256 706 3 443 3.37e-130 394
MS.gene65401.t1 AT3G17690 37.563 591 338 14 90 667 147 719 4.21e-119 375
MS.gene65401.t1 AT3G17700 37.544 570 328 10 101 658 192 745 3.76e-115 365
MS.gene65401.t1 AT5G15410 36.678 578 347 9 94 658 106 677 2.80e-108 346
MS.gene65401.t1 AT5G15410 36.678 578 347 9 94 658 105 676 3.00e-108 346
MS.gene65401.t1 AT5G54250 34.549 576 361 8 100 664 78 648 1.42e-107 343
MS.gene65401.t1 AT5G54250 34.549 576 361 8 100 664 78 648 1.42e-107 343
MS.gene65401.t1 AT5G54250 34.549 576 361 8 100 664 78 648 1.42e-107 343
MS.gene65401.t1 AT3G17690 37.897 504 291 10 90 589 147 632 9.99e-106 337
MS.gene65401.t1 AT3G17700 37.576 495 292 7 101 594 192 670 5.03e-103 331
MS.gene65401.t1 AT5G54250 33.854 576 360 9 100 664 78 643 4.07e-102 329
MS.gene65401.t1 AT3G17690 35.433 381 231 8 90 466 147 516 2.81e-66 228
MS.gene65401.t1 AT4G22200 24.020 204 152 2 379 582 263 463 2.28e-16 83.6
MS.gene65401.t1 AT4G22200 24.020 204 152 2 379 582 263 463 5.30e-16 82.8
MS.gene65401.t1 AT5G37500 21.702 470 273 13 99 561 55 436 8.09e-15 79.0
MS.gene65401.t1 AT5G37500 21.489 470 274 13 99 561 55 436 1.01e-14 79.0
MS.gene65401.t1 AT5G37500 20.726 468 280 12 99 561 55 436 1.32e-14 78.2
MS.gene65401.t1 AT5G37500 20.833 408 252 9 155 561 45 382 3.12e-14 77.0
MS.gene65401.t1 AT2G26650 23.902 205 153 2 379 582 239 441 3.87e-14 77.0
MS.gene65401.t1 AT2G26650 23.902 205 153 2 379 582 134 336 5.29e-14 76.3
MS.gene65401.t1 AT5G46240 22.727 198 152 1 379 576 246 442 5.60e-14 76.3
MS.gene65401.t1 AT3G02850 20.902 488 293 14 83 563 56 457 1.04e-13 75.5
MS.gene65401.t1 AT3G02850 20.902 488 293 14 83 563 56 457 1.26e-13 75.1
MS.gene65401.t1 AT3G02850 20.697 488 294 14 83 563 56 457 1.58e-13 74.7
MS.gene65401.t1 AT4G32650 23.153 203 153 2 380 582 276 475 1.58e-12 71.2
MS.gene65401.t1 AT4G32650 23.153 203 153 2 380 582 276 475 2.13e-12 71.2
MS.gene65401.t1 AT4G32650 23.153 203 153 2 380 582 276 475 2.27e-12 70.9

Find 148 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCATAGTAATAGCTATTA+TGG 0.126403 4.3:+88180398 MS.gene65401:CDS
GGCAAGCTTGCTGCAGATTT+TGG 0.164117 4.3:-88181153 None:intergenic
GGTGGGGACTTCAGAATTTA+AGG 0.170474 4.3:+88179942 MS.gene65401:CDS
CCTCAAAGTTCGTCAAAATT+TGG 0.172842 4.3:+88181456 MS.gene65401:CDS
GCACCTTCATCTCGAGTATT+TGG 0.204342 4.3:+88178822 MS.gene65401:CDS
CCATAGACAGTAACAAAATT+TGG 0.208171 4.3:-88179921 None:intergenic
TTCTGCAAGGGCGTTGGTTC+CGG 0.218060 4.3:+88181431 MS.gene65401:CDS
CTGCATGGAGGAAGCATTTA+AGG 0.231374 4.3:+88181349 MS.gene65401:CDS
TGTGACACTTTAAGATCTTC+TGG 0.248692 4.3:-88178561 None:intergenic
ATATCTCTTCTAAGATCTTT+TGG 0.250851 4.3:-88180734 None:intergenic
ATTTCGAGTCGGAGTGAAAA+AGG 0.263032 4.3:+88178476 MS.gene65401:CDS
ACTTGCTAGCTTGTTATCTA+TGG 0.279932 4.3:-88178721 None:intergenic
ATGATGTAGGTTGGATGATT+TGG 0.281948 4.3:+88178341 MS.gene65401:intron
CAGAGAAGACTTACATAGTT+AGG 0.283571 4.3:+88180980 MS.gene65401:CDS
AGGGGTCGATGAAGAAAATT+TGG 0.285665 4.3:+88180700 MS.gene65401:CDS
CCCCATAATAGCTATTACTA+TGG 0.288425 4.3:-88180400 None:intergenic
GATATTTCATTATTGACTTC+TGG 0.297261 4.3:+88178898 MS.gene65401:CDS
TCTGCAAGGGCGTTGGTTCC+GGG 0.297464 4.3:+88181432 MS.gene65401:CDS
AAATTTGGGGAAAACTTCTA+TGG 0.302110 4.3:-88179906 None:intergenic
CATAGACAGTAACAAAATTT+GGG 0.315147 4.3:-88179920 None:intergenic
GTGCTCTTTGCTCTTCTGAT+TGG 0.318517 4.3:+88180426 MS.gene65401:CDS
CAATGAAGGCGGTGCGGAAT+TGG 0.323385 4.3:-88178800 None:intergenic
TCCATAGTAATAGCTATTAT+GGG 0.333113 4.3:+88180399 MS.gene65401:CDS
AATCAGGTTCTGAGGGCTTT+GGG 0.347993 4.3:-88181569 None:intergenic
CGCAAGACGCATGGAGGTAT+TGG 0.348515 4.3:+88179068 MS.gene65401:CDS
AAATCAGGTTCTGAGGGCTT+TGG 0.350213 4.3:-88181570 None:intergenic
TTCATCCCACTTGAGCTAGA+TGG 0.351952 4.3:-88178381 None:intergenic
TCAGATGGACTAATGAATAT+TGG 0.360085 4.3:+88178501 MS.gene65401:CDS
TCAATCAATGTCGGTTCGTC+TGG 0.370374 4.3:+88180562 MS.gene65401:CDS
GCTAGATGGCATTGACGAAT+TGG 0.374592 4.3:-88178367 None:intergenic
AATGGTACGGTTCATACCTT+TGG 0.384471 4.3:+88179140 MS.gene65401:CDS
ATACAACAGTCTATGCTTCT+CGG 0.384722 4.3:+88181484 MS.gene65401:CDS
TCATTGAGAGCACTGAAATC+AGG 0.395867 4.3:-88181585 None:intergenic
ATGAACAACATCTCATCAAC+TGG 0.395916 4.3:-88181012 None:intergenic
AAGATAAGAATCTTCTGTAT+TGG 0.406036 4.3:+88178604 MS.gene65401:CDS
CTTGCAGAATCATCACTTCC+CGG 0.414286 4.3:-88181417 None:intergenic
CGGTTTGCTGCTAATCTTCG+TGG 0.417955 4.3:+88181504 MS.gene65401:CDS
TTCACGGTTCTTGTTGATGA+TGG 0.422131 4.3:-88181174 None:intergenic
TACCTCACTCATAGCTTTCA+CGG 0.425446 4.3:-88181190 None:intergenic
TACGATTTGTGGTTGAAATA+CGG 0.428941 4.3:-88178690 None:intergenic
GTCTGTACTGCCACTGCCAC+AGG 0.435030 4.3:+88178920 MS.gene65401:CDS
CACTCAAAAGTGGAGTCACT+TGG 0.446602 4.3:+88178529 MS.gene65401:CDS
TCCATTGTTGCGAATAGAAA+CGG 0.447494 4.3:-88181299 None:intergenic
GTGCCATGTACTATTTGATC+TGG 0.449942 4.3:+88179214 MS.gene65401:CDS
GGGTGTTCAACCACTTGTAT+TGG 0.454525 4.3:-88180676 None:intergenic
ATGGAAAACAAAACCTCTCC+AGG 0.456409 4.3:-88180381 None:intergenic
TGGCTAAACAATACGATTTG+TGG 0.457799 4.3:-88178701 None:intergenic
GGTATGAACCGTACCATTCT+CGG 0.464303 4.3:-88179135 None:intergenic
GCATCATCGCTTGCTTCCTC+CGG 0.465125 4.3:+88180625 MS.gene65401:CDS
GCTTGCTTCCTCCGGACCTA+AGG 0.465489 4.3:+88180633 MS.gene65401:CDS
GGTGAACTTGTCATAGATCC+TGG 0.473833 4.3:+88178849 MS.gene65401:CDS
CCGCGGCCGGGAATAGGAAA+AGG 0.476908 4.3:-88178432 None:intergenic
TTCACCAGCTCTCTTGAACT+TGG 0.484399 4.3:-88181543 None:intergenic
CTGACTCTTTCCCTTAGGTC+CGG 0.486118 4.3:-88180644 None:intergenic
AGCGTCTGACTCTTTCCCTT+AGG 0.488363 4.3:-88180649 None:intergenic
TTCTTCACAATGTTTGACTC+TGG 0.491771 4.3:+88177915 None:intergenic
AGAGAAGACTTACATAGTTA+GGG 0.493349 4.3:+88180981 MS.gene65401:CDS
TATCGCTTTGCGATCAATCC+AGG 0.494047 4.3:-88178867 None:intergenic
TAGAACGTAACGACACATGC+TGG 0.500625 4.3:+88179684 MS.gene65401:CDS
GAAAGCATGTAAAGAAGTTG+AGG 0.502958 4.3:+88179709 MS.gene65401:CDS
GGTTCCTCTCTTTGCTAACA+TGG 0.504157 4.3:+88180906 MS.gene65401:intron
GGCATTTGCTTTAGAAGCAG+AGG 0.505619 4.3:+88181215 MS.gene65401:CDS
AATTCACATTTCTTGTATTG+TGG 0.506801 4.3:+88179737 MS.gene65401:CDS
TTTGTTTGAATTTAGTCAGA+AGG 0.518058 4.3:+88180768 MS.gene65401:CDS
ACTCAAAAGTGGAGTCACTT+GGG 0.520420 4.3:+88178530 MS.gene65401:CDS
TACAGAAGATTCTTATCTTG+AGG 0.521699 4.3:-88178600 None:intergenic
TCGAGATGAAGGTGCAATGA+AGG 0.522257 4.3:-88178814 None:intergenic
ATTCGCAACAATGGAGAACA+TGG 0.522904 4.3:+88181307 MS.gene65401:CDS
GACGAACCGACATTGATTGA+AGG 0.524267 4.3:-88180559 None:intergenic
GTATCTCAAACCTGTGGCAG+TGG 0.526783 4.3:-88178930 None:intergenic
TGGAGGAAATGAGGATCAAA+AGG 0.526808 4.3:+88180582 MS.gene65401:CDS
TCCGTTTCTATTCGCAACAA+TGG 0.528384 4.3:+88181298 MS.gene65401:CDS
TCTTTATAGCGCAAGACGCA+TGG 0.530371 4.3:+88179059 MS.gene65401:CDS
AGAGTTGCTAACATGGGCAT+TGG 0.533440 4.3:+88181128 MS.gene65401:CDS
TGATGATTCTGCAAGGGCGT+TGG 0.534637 4.3:+88181425 MS.gene65401:CDS
TCATCTCGAGTATTTGGAAG+AGG 0.535503 4.3:+88178828 MS.gene65401:CDS
TTTCACAGTACACTTGGTCA+GGG 0.537219 4.3:+88180339 MS.gene65401:intron
TACAAGTCACAGTATATTGA+AGG 0.537328 4.3:+88177939 MS.gene65401:CDS
GAAAGCTATGAGTGAGGTAG+AGG 0.537681 4.3:+88181194 MS.gene65401:CDS
GAGATGTTGTTCATCATTCG+TGG 0.539150 4.3:+88181021 MS.gene65401:CDS
CTAGAGAGTGTTACTACAGA+TGG 0.541421 4.3:+88181048 MS.gene65401:CDS
AGTTCAAGTAAGCATATACC+AGG 0.542819 4.3:+88179761 MS.gene65401:CDS
GTCTTCTCTGTATATAAACT+CGG 0.543647 4.3:-88180967 None:intergenic
TAGACACATACATAGTAGAC+AGG 0.544177 4.3:+88181263 MS.gene65401:CDS
ACAGTATATTGAAGGTCAGA+AGG 0.547235 4.3:+88177947 MS.gene65401:CDS
ATCAATGTCGGTTCGTCTGG+AGG 0.548219 4.3:+88180565 MS.gene65401:CDS
TTGTTTGAATTTAGTCAGAA+GGG 0.551796 4.3:+88180769 MS.gene65401:CDS
GCACTGAAATCAGGTTCTGA+GGG 0.551873 4.3:-88181576 None:intergenic
AGAAGTGGCTTCTTCAACAG+AGG 0.551875 4.3:+88181075 MS.gene65401:CDS
CTTGCTTCCTCCGGACCTAA+GGG 0.552988 4.3:+88180634 MS.gene65401:CDS
ATTGGAAGATCACTCAAAAG+TGG 0.553145 4.3:+88178519 MS.gene65401:CDS
AGCACTGAAATCAGGTTCTG+AGG 0.553944 4.3:-88181577 None:intergenic
TTTGACGAACTTTGAGGACC+CGG 0.557037 4.3:-88181450 None:intergenic
AAAGCATGTAAAGAAGTTGA+GGG 0.558423 4.3:+88179710 MS.gene65401:CDS
TTGCAGAATCATCACTTCCC+GGG 0.558557 4.3:-88181416 None:intergenic
CGGTTCGTCTGGAGGAAATG+AGG 0.562037 4.3:+88180573 MS.gene65401:CDS
AGTACCAAGTTCAAGAGAGC+TGG 0.565279 4.3:+88181539 MS.gene65401:CDS
CTTCCAAATACTCGAGATGA+AGG 0.565402 4.3:-88178825 None:intergenic
TCTTCTGATTGGAAACATGC+AGG 0.565687 4.3:+88180437 MS.gene65401:CDS
ACTACAGATGGTGGAAGAAG+TGG 0.567107 4.3:+88181060 MS.gene65401:CDS
TGTTGTAGATTGTAGTGTGG+AGG 0.567262 4.3:+88179034 MS.gene65401:intron
TTCACAGTACACTTGGTCAG+GGG 0.577586 4.3:+88180340 MS.gene65401:intron
TCAATGCCATCTAGCTCAAG+TGG 0.579410 4.3:+88178375 MS.gene65401:CDS
TTTAAGGCGAAGGCGAAGGA+AGG 0.579780 4.3:+88181365 MS.gene65401:CDS
CCATAGTAATAGCTATTATG+GGG 0.581237 4.3:+88180400 MS.gene65401:CDS
CTCAAAAGTGGAGTCACTTG+GGG 0.583096 4.3:+88178531 MS.gene65401:CDS
AGCATTTAAGGCGAAGGCGA+AGG 0.589667 4.3:+88181361 MS.gene65401:CDS
CAATCTATATTCAAGCTGCA+TGG 0.590954 4.3:+88181334 MS.gene65401:CDS
GGAGGAAGCATTTAAGGCGA+AGG 0.591557 4.3:+88181355 MS.gene65401:CDS
ATATTACGAATCTGATTACC+CGG 0.594747 4.3:+88181398 MS.gene65401:CDS
GCGATGATGCATCCACTGCT+CGG 0.598686 4.3:-88180612 None:intergenic
ATATACCAGGGTATGACAGT+TGG 0.606713 4.3:+88179774 MS.gene65401:CDS
GTTTGTTGTAGATTGTAGTG+TGG 0.607307 4.3:+88179031 MS.gene65401:intron
GACTAGTACCTACCCTGGAG+AGG 0.607375 4.3:+88180368 MS.gene65401:CDS
GAGAGTGTTACTACAGATGG+TGG 0.613056 4.3:+88181051 MS.gene65401:CDS
GACCTACCTTCAATCAATGT+CGG 0.613772 4.3:+88180553 MS.gene65401:intron
AAGGCGAAGGCGAAGGAAGG+AGG 0.615424 4.3:+88181368 MS.gene65401:CDS
ACTCTTTCCCTTAGGTCCGG+AGG 0.617015 4.3:-88180641 None:intergenic
AAAGGCGTGACTCCGAGCAG+TGG 0.621729 4.3:+88180600 MS.gene65401:CDS
AAACAAAACCTCTCCAGGGT+AGG 0.625990 4.3:-88180376 None:intergenic
GTGGTGAAGAGTTGCTAACA+TGG 0.626286 4.3:+88181121 MS.gene65401:CDS
CGGGAAGTGATGATTCTGCA+AGG 0.626896 4.3:+88181418 MS.gene65401:CDS
AAGACTTACATAGTTAGGGA+AGG 0.629987 4.3:+88180985 MS.gene65401:CDS
TACAAGTGGTTGAACACCCG+AGG 0.630986 4.3:+88180680 MS.gene65401:CDS
GACGCTACGACCAATACAAG+TGG 0.637852 4.3:+88180666 MS.gene65401:CDS
TGGAAAACAAAACCTCTCCA+GGG 0.644846 4.3:-88180380 None:intergenic
TCGTCGAAATCATTTCGAGT+CGG 0.644882 4.3:+88178465 MS.gene65401:CDS
TCGTCCATGTTAGCAAAGAG+AGG 0.645418 4.3:-88180910 None:intergenic
GGGAAGTGATGATTCTGCAA+GGG 0.645571 4.3:+88181419 MS.gene65401:CDS
ATACCAGATCAAATAGTACA+TGG 0.646335 4.3:-88179217 None:intergenic
TTTCTCCAACTGTCATACCC+TGG 0.659364 4.3:-88179779 None:intergenic
CAATGCCATCTAGCTCAAGT+GGG 0.666519 4.3:+88178376 MS.gene65401:CDS
ATAGACAGTAACAAAATTTG+GGG 0.668163 4.3:-88179919 None:intergenic
GTCAGAAGGAGAAATTTGTG+AGG 0.668583 4.3:+88177961 MS.gene65401:CDS
TGTAGATAACGGGTTCTGTG+TGG 0.671626 4.3:+88179648 MS.gene65401:intron
CTTCTGACTAGTACCTACCC+TGG 0.672528 4.3:+88180363 MS.gene65401:CDS
AGATGAAGGTGCAATGAAGG+CGG 0.673510 4.3:-88178811 None:intergenic
ACAAGTGGTTGAACACCCGA+GGG 0.676415 4.3:+88180681 MS.gene65401:CDS
AACCGTGAAAGCTATGAGTG+AGG 0.683734 4.3:+88181188 MS.gene65401:CDS
TTCGCAACAATGGAGAACAT+GGG 0.684012 4.3:+88181308 MS.gene65401:CDS
TGGTGAAGAGTTGCTAACAT+GGG 0.697702 4.3:+88181122 MS.gene65401:CDS
AAGGTGCAATGAAGGCGGTG+CGG 0.699069 4.3:-88178806 None:intergenic
TATTACGAATCTGATTACCC+GGG 0.702346 4.3:+88181399 MS.gene65401:CDS
AACCGACATTGATTGAAGGT+AGG 0.702813 4.3:-88180555 None:intergenic
TCTATATTCAAGCTGCATGG+AGG 0.707083 4.3:+88181337 MS.gene65401:CDS
TTATAGCGCAAGACGCATGG+AGG 0.742423 4.3:+88179062 MS.gene65401:CDS
GTTCAAGTAAGCATATACCA+GGG 0.749555 4.3:+88179762 MS.gene65401:CDS
CAAGTGGTTGAACACCCGAG+GGG 0.768003 4.3:+88180682 MS.gene65401:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATATATGTATTAGTGT+CGG + chr4.3:88180065-88180084 MS.gene65401:intron 15.0%
!! ATATTTATAAAGGATGATAT+AGG - chr4.3:88180236-88180255 None:intergenic 15.0%
!!! AACTATTATTTTGTAGATAA+CGG + chr4.3:88179637-88179656 MS.gene65401:intron 15.0%
!! ATGTTTACAGTAAAAACTTT+CGG - chr4.3:88180467-88180486 None:intergenic 20.0%
!! GAATCAAAGCATATTTATAA+AGG - chr4.3:88180246-88180265 None:intergenic 20.0%
!!! ACTATTATTTTGTAGATAAC+GGG + chr4.3:88179638-88179657 MS.gene65401:intron 20.0%
!!! TATATTCCACTTTAAGTTTA+GGG + chr4.3:88178265-88178284 MS.gene65401:intron 20.0%
!!! TATTGGTTAATTTAAGAACA+TGG + chr4.3:88178204-88178223 MS.gene65401:intron 20.0%
!!! TGTGAGGTATTTTTATTTTT+GGG + chr4.3:88177977-88177996 MS.gene65401:intron 20.0%
!!! TTATAAACTGAGTGTTTTAT+AGG - chr4.3:88180028-88180047 None:intergenic 20.0%
!!! TTATATTCCACTTTAAGTTT+AGG + chr4.3:88178264-88178283 MS.gene65401:intron 20.0%
!!! TTGTGAGGTATTTTTATTTT+TGG + chr4.3:88177976-88177995 MS.gene65401:intron 20.0%
! AAGATAAGAATCTTCTGTAT+TGG + chr4.3:88178604-88178623 MS.gene65401:CDS 25.0%
! AATTCACATTTCTTGTATTG+TGG + chr4.3:88179737-88179756 MS.gene65401:CDS 25.0%
! AGATGCTTATGTTAACAAAT+TGG - chr4.3:88178301-88178320 None:intergenic 25.0%
! ATAGACAGTAACAAAATTTG+GGG - chr4.3:88179922-88179941 None:intergenic 25.0%
! CAACAAACATTAAGACATAT+TGG - chr4.3:88179019-88179038 None:intergenic 25.0%
! CATAGACAGTAACAAAATTT+GGG - chr4.3:88179923-88179942 None:intergenic 25.0%
! CTCCATAAAAGAAAAAATGT+TGG + chr4.3:88179329-88179348 MS.gene65401:intron 25.0%
! GTATAACATATCAATGTGAT+TGG + chr4.3:88177999-88178018 MS.gene65401:intron 25.0%
! GTGTATGACATATATTATTC+AGG + chr4.3:88180885-88180904 MS.gene65401:intron 25.0%
! TATCATTCACATATTCTCTT+TGG + chr4.3:88179369-88179388 MS.gene65401:intron 25.0%
! TCCATAGTAATAGCTATTAT+GGG + chr4.3:88180399-88180418 MS.gene65401:CDS 25.0%
! TCTTAAGAAATGAACTTACA+TGG - chr4.3:88179254-88179273 None:intergenic 25.0%
! TGAGAAAATAAAGAGTTAGA+AGG - chr4.3:88180855-88180874 None:intergenic 25.0%
! TTCCATAGTAATAGCTATTA+TGG + chr4.3:88180398-88180417 MS.gene65401:CDS 25.0%
! TTGTAGATATTCTGACATAA+AGG + chr4.3:88179541-88179560 MS.gene65401:intron 25.0%
! TTGTTTGAATTTAGTCAGAA+GGG + chr4.3:88180769-88180788 MS.gene65401:CDS 25.0%
! TTTGTTTGAATTTAGTCAGA+AGG + chr4.3:88180768-88180787 MS.gene65401:CDS 25.0%
!! TAGAAAAATTGGATGCTTTT+TGG - chr4.3:88179413-88179432 None:intergenic 25.0%
!!! AAAAGCATCCAATTTTTCTA+TGG + chr4.3:88179413-88179432 MS.gene65401:intron 25.0%
!!! AATAAATTTTGGTGTCCAAT+CGG - chr4.3:88179486-88179505 None:intergenic 25.0%
!!! ATATCTCTTCTAAGATCTTT+TGG - chr4.3:88180737-88180756 None:intergenic 25.0%
!!! ATGAGGAGTATCAATTTTAA+TGG - chr4.3:88179446-88179465 None:intergenic 25.0%
!!! CACCAACATTTTTTCTTTTA+TGG - chr4.3:88179334-88179353 None:intergenic 25.0%
!!! GATATTTCATTATTGACTTC+TGG + chr4.3:88178898-88178917 MS.gene65401:CDS 25.0%
!!! GTTTTATAGGTGTTATAAAG+AGG - chr4.3:88180015-88180034 None:intergenic 25.0%
!!! TGAGGAGTATCAATTTTAAT+GGG - chr4.3:88179445-88179464 None:intergenic 25.0%
AAAGCATGTAAAGAAGTTGA+GGG + chr4.3:88179710-88179729 MS.gene65401:CDS 30.0%
AAATTTGGGGAAAACTTCTA+TGG - chr4.3:88179909-88179928 None:intergenic 30.0%
AAGCAAAACAGAAGGATATT+TGG - chr4.3:88180320-88180339 None:intergenic 30.0%
AATTAACCAATAGAACCAAG+TGG - chr4.3:88178196-88178215 None:intergenic 30.0%
AGAGAAGACTTACATAGTTA+GGG + chr4.3:88180981-88181000 MS.gene65401:CDS 30.0%
ATACCAGATCAAATAGTACA+TGG - chr4.3:88179220-88179239 None:intergenic 30.0%
ATATTACGAATCTGATTACC+CGG + chr4.3:88181398-88181417 MS.gene65401:CDS 30.0%
ATGGCTAATGTGAAAAACAT+AGG - chr4.3:88180094-88180113 None:intergenic 30.0%
ATTGTAGATTCACTTGATGT+TGG + chr4.3:88180205-88180224 MS.gene65401:intron 30.0%
CCAATTGCATTTATAACACA+AGG - chr4.3:88180530-88180549 None:intergenic 30.0%
CCATAGACAGTAACAAAATT+TGG - chr4.3:88179924-88179943 None:intergenic 30.0%
CCATAGTAATAGCTATTATG+GGG + chr4.3:88180400-88180419 MS.gene65401:CDS 30.0%
CCTTGTGTTATAAATGCAAT+TGG + chr4.3:88180527-88180546 MS.gene65401:intron 30.0%
CTCGATAGATTTGTCATTAA+CGG + chr4.3:88178159-88178178 MS.gene65401:intron 30.0%
GGAAGTTTCCATAGAAAAAT+TGG - chr4.3:88179424-88179443 None:intergenic 30.0%
GTCTTCTCTGTATATAAACT+CGG - chr4.3:88180970-88180989 None:intergenic 30.0%
GTTAGAATAGATTACGCTAT+AGG + chr4.3:88179563-88179582 MS.gene65401:intron 30.0%
TAATATACAGTGGATGATGT+AGG + chr4.3:88178328-88178347 MS.gene65401:intron 30.0%
TACAAGTCACAGTATATTGA+AGG + chr4.3:88177939-88177958 MS.gene65401:CDS 30.0%
TACAGAAGATTCTTATCTTG+AGG - chr4.3:88178603-88178622 None:intergenic 30.0%
TACGATTTGTGGTTGAAATA+CGG - chr4.3:88178693-88178712 None:intergenic 30.0%
TACGGAAGATAGAAAAAGAA+AGG - chr4.3:88178675-88178694 None:intergenic 30.0%
TCTGATCTTCATATCAAAGT+CGG + chr4.3:88180142-88180161 MS.gene65401:intron 30.0%
TCTTTCAGACTAATATACAG+TGG + chr4.3:88178318-88178337 MS.gene65401:intron 30.0%
TTGTAGATTCACTTGATGTT+GGG + chr4.3:88180206-88180225 MS.gene65401:intron 30.0%
! AATATTTTCCGAGAATGGTA+CGG + chr4.3:88179127-88179146 MS.gene65401:CDS 30.0%
! AATTTTGTTACTGTCTATGG+TGG + chr4.3:88179924-88179943 MS.gene65401:CDS 30.0%
! ATTTTGTTACTGTCTATGGT+GGG + chr4.3:88179925-88179944 MS.gene65401:CDS 30.0%
! CCAAATTTTGTTACTGTCTA+TGG + chr4.3:88179921-88179940 MS.gene65401:CDS 30.0%
! GACTCTATTAATCTGGATTT+TGG + chr4.3:88178074-88178093 MS.gene65401:intron 30.0%
!! GATAACTCAGCTTTTAACTA+TGG + chr4.3:88179845-88179864 MS.gene65401:CDS 30.0%
!! TATTAATCTGGATTTTGGTC+AGG + chr4.3:88178079-88178098 MS.gene65401:intron 30.0%
!! TCAGATGGACTAATGAATAT+TGG + chr4.3:88178501-88178520 MS.gene65401:CDS 30.0%
!! TTTTCTGAAAATGCATTGCT+TGG + chr4.3:88179192-88179211 MS.gene65401:CDS 30.0%
!!! ATAAATTTTGGTGTCCAATC+GGG - chr4.3:88179485-88179504 None:intergenic 30.0%
!!! TTTTTAACTTCTGAAGCCAA+AGG - chr4.3:88179159-88179178 None:intergenic 30.0%
AAGACTTACATAGTTAGGGA+AGG + chr4.3:88180985-88181004 MS.gene65401:CDS 35.0%
ACAGTATATTGAAGGTCAGA+AGG + chr4.3:88177947-88177966 MS.gene65401:CDS 35.0%
ACTGTGAAAAGCAAAACAGA+AGG - chr4.3:88180328-88180347 None:intergenic 35.0%
AGAATAGATTACGCTATAGG+CGG + chr4.3:88179566-88179585 MS.gene65401:intron 35.0%
AGTTCAAGTAAGCATATACC+AGG + chr4.3:88179761-88179780 MS.gene65401:CDS 35.0%
ATACAACAGTCTATGCTTCT+CGG + chr4.3:88181484-88181503 MS.gene65401:CDS 35.0%
ATGAACAACATCTCATCAAC+TGG - chr4.3:88181015-88181034 None:intergenic 35.0%
ATTGGAAGATCACTCAAAAG+TGG + chr4.3:88178519-88178538 MS.gene65401:CDS 35.0%
CAAAACATATTGAGCATGGA+TGG - chr4.3:88178955-88178974 None:intergenic 35.0%
CAATCTATATTCAAGCTGCA+TGG + chr4.3:88181334-88181353 MS.gene65401:CDS 35.0%
CAGAGAAGACTTACATAGTT+AGG + chr4.3:88180980-88180999 MS.gene65401:CDS 35.0%
CATAAGTGATACAGAAGCAA+TGG - chr4.3:88179298-88179317 None:intergenic 35.0%
CCCCATAATAGCTATTACTA+TGG - chr4.3:88180403-88180422 None:intergenic 35.0%
CCTCAAAGTTCGTCAAAATT+TGG + chr4.3:88181456-88181475 MS.gene65401:CDS 35.0%
GAAACATTGATCATTCGTGT+CGG - chr4.3:88179612-88179631 None:intergenic 35.0%
GAACCAGAGAGTTAAGATAA+CGG - chr4.3:88178041-88178060 None:intergenic 35.0%
GGAAAGAGACTCTATTAATC+TGG + chr4.3:88178067-88178086 MS.gene65401:intron 35.0%
GTTCAAGTAAGCATATACCA+GGG + chr4.3:88179762-88179781 MS.gene65401:CDS 35.0%
TACGCAAAACATATTGAGCA+TGG - chr4.3:88178959-88178978 None:intergenic 35.0%
TAGACACATACATAGTAGAC+AGG + chr4.3:88181263-88181282 MS.gene65401:CDS 35.0%
TATTACGAATCTGATTACCC+GGG + chr4.3:88181399-88181418 MS.gene65401:CDS 35.0%
TCAGAAGTTAAAAAGACAGC+TGG + chr4.3:88179165-88179184 MS.gene65401:CDS 35.0%
TCCATTGTTGCGAATAGAAA+CGG - chr4.3:88181302-88181321 None:intergenic 35.0%
TCTGTATCACTTATGAAGCT+CGG + chr4.3:88179303-88179322 MS.gene65401:intron 35.0%
TGCAATGAGAAATCTCAGAA+AGG - chr4.3:88180819-88180838 None:intergenic 35.0%
TGCATACCCTAAACTTAAAG+TGG - chr4.3:88178274-88178293 None:intergenic 35.0%
TGGCTAAACAATACGATTTG+TGG - chr4.3:88178704-88178723 None:intergenic 35.0%
TGTTATAAAGAGGACTCATG+TGG - chr4.3:88180005-88180024 None:intergenic 35.0%
TTTCCGTTATCTTAACTCTC+TGG + chr4.3:88178035-88178054 MS.gene65401:intron 35.0%
! AATCTATCGAGAGTATATCC+AGG - chr4.3:88178150-88178169 None:intergenic 35.0%
! ATGATGTAGGTTGGATGATT+TGG + chr4.3:88178341-88178360 MS.gene65401:intron 35.0%
! CATGCAATATTTTCCGAGAA+TGG + chr4.3:88179122-88179141 MS.gene65401:CDS 35.0%
! GAAAGCATGTAAAGAAGTTG+AGG + chr4.3:88179709-88179728 MS.gene65401:CDS 35.0%
! GAAGTATCAAGAAGTGTATG+AGG - chr4.3:88179463-88179482 None:intergenic 35.0%
! TTGAAGGAAGGTGATTTTTG+TGG + chr4.3:88181102-88181121 MS.gene65401:CDS 35.0%
! TTTTCACATTAGCCATGTAG+AGG + chr4.3:88180098-88180117 MS.gene65401:intron 35.0%
! TTTTGTTACTGTCTATGGTG+GGG + chr4.3:88179926-88179945 MS.gene65401:CDS 35.0%
!! ACTTGCTAGCTTGTTATCTA+TGG - chr4.3:88178724-88178743 None:intergenic 35.0%
!! CATTAACCACTTGGTTCTAT+TGG + chr4.3:88178187-88178206 MS.gene65401:intron 35.0%
!! CCAAATTTTGACGAACTTTG+AGG - chr4.3:88181459-88181478 None:intergenic 35.0%
!! GTTTGTTGTAGATTGTAGTG+TGG + chr4.3:88179031-88179050 MS.gene65401:intron 35.0%
!! TATCTTAACTCTCTGGTTCT+CGG + chr4.3:88178042-88178061 MS.gene65401:intron 35.0%
!! TGTGACACTTTAAGATCTTC+TGG - chr4.3:88178564-88178583 None:intergenic 35.0%
!! TTTGCTTTTCACAGTACACT+TGG + chr4.3:88180333-88180352 MS.gene65401:intron 35.0%
AATGGTACGGTTCATACCTT+TGG + chr4.3:88179140-88179159 MS.gene65401:CDS 40.0%
ACGGAAGTTCATTAACCACT+TGG + chr4.3:88178178-88178197 MS.gene65401:intron 40.0%
ACTCAAAAGTGGAGTCACTT+GGG + chr4.3:88178530-88178549 MS.gene65401:CDS 40.0%
ACTTCTTGATACTTCCCGAT+TGG + chr4.3:88179468-88179487 MS.gene65401:intron 40.0%
AGGGGTCGATGAAGAAAATT+TGG + chr4.3:88180700-88180719 MS.gene65401:CDS 40.0%
ATACAGTGGATGATGTAGGT+TGG + chr4.3:88178332-88178351 MS.gene65401:intron 40.0%
ATATACCAGGGTATGACAGT+TGG + chr4.3:88179774-88179793 MS.gene65401:CDS 40.0%
ATGGAAAACAAAACCTCTCC+AGG - chr4.3:88180384-88180403 None:intergenic 40.0%
ATTCGCAACAATGGAGAACA+TGG + chr4.3:88181307-88181326 MS.gene65401:CDS 40.0%
CTTCCAAATACTCGAGATGA+AGG - chr4.3:88178828-88178847 None:intergenic 40.0%
GAAGATCAGACACACGATAT+GGG - chr4.3:88180132-88180151 None:intergenic 40.0%
GACCTACCTTCAATCAATGT+CGG + chr4.3:88180553-88180572 MS.gene65401:intron 40.0%
GAGATGTTGTTCATCATTCG+TGG + chr4.3:88181021-88181040 MS.gene65401:CDS 40.0%
GTCAGAAGGAGAAATTTGTG+AGG + chr4.3:88177961-88177980 MS.gene65401:CDS 40.0%
GTGCCATGTACTATTTGATC+TGG + chr4.3:88179214-88179233 MS.gene65401:CDS 40.0%
TACCTCACTCATAGCTTTCA+CGG - chr4.3:88181193-88181212 None:intergenic 40.0%
TCATCTCGAGTATTTGGAAG+AGG + chr4.3:88178828-88178847 MS.gene65401:CDS 40.0%
TCCGTTTCTATTCGCAACAA+TGG + chr4.3:88181298-88181317 MS.gene65401:CDS 40.0%
TCTATATTCAAGCTGCATGG+AGG + chr4.3:88181337-88181356 MS.gene65401:CDS 40.0%
TCTTCTGATTGGAAACATGC+AGG + chr4.3:88180437-88180456 MS.gene65401:CDS 40.0%
TGAAGATCAGACACACGATA+TGG - chr4.3:88180133-88180152 None:intergenic 40.0%
TGGAAAACAAAACCTCTCCA+GGG - chr4.3:88180383-88180402 None:intergenic 40.0%
TGGAGGAAATGAGGATCAAA+AGG + chr4.3:88180582-88180601 MS.gene65401:CDS 40.0%
TGGTGAAGAGTTGCTAACAT+GGG + chr4.3:88181122-88181141 MS.gene65401:CDS 40.0%
TGTTGTAGATTGTAGTGTGG+AGG + chr4.3:88179034-88179053 MS.gene65401:intron 40.0%
TTCGCAACAATGGAGAACAT+GGG + chr4.3:88181308-88181327 MS.gene65401:CDS 40.0%
TTTCACAGTACACTTGGTCA+GGG + chr4.3:88180339-88180358 MS.gene65401:intron 40.0%
! AACCGACATTGATTGAAGGT+AGG - chr4.3:88180558-88180577 None:intergenic 40.0%
! AATTTTCTTCATCGACCCCT+CGG - chr4.3:88180700-88180719 None:intergenic 40.0%
! ATCGAGAGTATATCCAGGTT+CGG - chr4.3:88178145-88178164 None:intergenic 40.0%
! ATTTCGAGTCGGAGTGAAAA+AGG + chr4.3:88178476-88178495 MS.gene65401:CDS 40.0%
! TCGTCGAAATCATTTCGAGT+CGG + chr4.3:88178465-88178484 MS.gene65401:CDS 40.0%
! TGTTTGCCTTTTCCTATTCC+CGG + chr4.3:88178426-88178445 MS.gene65401:CDS 40.0%
! TTCACGGTTCTTGTTGATGA+TGG - chr4.3:88181177-88181196 None:intergenic 40.0%
! TTTCGAGTCGGAGTGAAAAA+GGG + chr4.3:88178477-88178496 MS.gene65401:CDS 40.0%
! TTTTCACAGTACACTTGGTC+AGG + chr4.3:88180338-88180357 MS.gene65401:intron 40.0%
!! ATCTTAACTCTCTGGTTCTC+GGG + chr4.3:88178043-88178062 MS.gene65401:intron 40.0%
!! CTAGAGAGTGTTACTACAGA+TGG + chr4.3:88181048-88181067 MS.gene65401:CDS 40.0%
!! CTTCAACAGAGGAGTTTTGA+AGG + chr4.3:88181086-88181105 MS.gene65401:CDS 40.0%
!! TCATTGAGAGCACTGAAATC+AGG - chr4.3:88181588-88181607 None:intergenic 40.0%
!!! AACAGAGGAGTTTTGAAGGA+AGG + chr4.3:88181090-88181109 MS.gene65401:CDS 40.0%
!!! TCGACGAATTTTTTTTCCCG+CGG - chr4.3:88178452-88178471 None:intergenic 40.0%
AAACAAAACCTCTCCAGGGT+AGG - chr4.3:88180379-88180398 None:intergenic 45.0%
AACCGTGAAAGCTATGAGTG+AGG + chr4.3:88181188-88181207 MS.gene65401:CDS 45.0%
AGAAGTGGCTTCTTCAACAG+AGG + chr4.3:88181075-88181094 MS.gene65401:CDS 45.0%
AGAGTTGCTAACATGGGCAT+TGG + chr4.3:88181128-88181147 MS.gene65401:CDS 45.0%
AGTACCAAGTTCAAGAGAGC+TGG + chr4.3:88181539-88181558 MS.gene65401:CDS 45.0%
CACTCAAAAGTGGAGTCACT+TGG + chr4.3:88178529-88178548 MS.gene65401:CDS 45.0%
CTCAAAAGTGGAGTCACTTG+GGG + chr4.3:88178531-88178550 MS.gene65401:CDS 45.0%
CTTGCAGAATCATCACTTCC+CGG - chr4.3:88181420-88181439 None:intergenic 45.0%
GAAAGCTATGAGTGAGGTAG+AGG + chr4.3:88181194-88181213 MS.gene65401:CDS 45.0%
GCACCTTCATCTCGAGTATT+TGG + chr4.3:88178822-88178841 MS.gene65401:CDS 45.0%
GGAGTGAAAAAGGGATCAGA+TGG + chr4.3:88178486-88178505 MS.gene65401:CDS 45.0%
GGGAAGTGATGATTCTGCAA+GGG + chr4.3:88181419-88181438 MS.gene65401:CDS 45.0%
GGGTGTTCAACCACTTGTAT+TGG - chr4.3:88180679-88180698 None:intergenic 45.0%
GGTATGAACCGTACCATTCT+CGG - chr4.3:88179138-88179157 None:intergenic 45.0%
GGTGAACTTGTCATAGATCC+TGG + chr4.3:88178849-88178868 MS.gene65401:CDS 45.0%
GGTGGGGACTTCAGAATTTA+AGG + chr4.3:88179942-88179961 MS.gene65401:CDS 45.0%
GGTTCCTCTCTTTGCTAACA+TGG + chr4.3:88180906-88180925 MS.gene65401:intron 45.0%
GTGCTCTTTGCTCTTCTGAT+TGG + chr4.3:88180426-88180445 MS.gene65401:CDS 45.0%
GTGGTGAAGAGTTGCTAACA+TGG + chr4.3:88181121-88181140 MS.gene65401:CDS 45.0%
TAGAACGTAACGACACATGC+TGG + chr4.3:88179684-88179703 MS.gene65401:CDS 45.0%
TATCGCTTTGCGATCAATCC+AGG - chr4.3:88178870-88178889 None:intergenic 45.0%
TCGTCCATGTTAGCAAAGAG+AGG - chr4.3:88180913-88180932 None:intergenic 45.0%
TCTTTATAGCGCAAGACGCA+TGG + chr4.3:88179059-88179078 MS.gene65401:CDS 45.0%
TGGATGGTATCTCAAACCTG+TGG - chr4.3:88178939-88178958 None:intergenic 45.0%
TTCACAGTACACTTGGTCAG+GGG + chr4.3:88180340-88180359 MS.gene65401:intron 45.0%
TTCACCAGCTCTCTTGAACT+TGG - chr4.3:88181546-88181565 None:intergenic 45.0%
TTCATCCCACTTGAGCTAGA+TGG - chr4.3:88178384-88178403 None:intergenic 45.0%
TTGCAGAATCATCACTTCCC+GGG - chr4.3:88181419-88181438 None:intergenic 45.0%
TTTCTCCAACTGTCATACCC+TGG - chr4.3:88179782-88179801 None:intergenic 45.0%
! AGATGAAGGTGCAATGAAGG+CGG - chr4.3:88178814-88178833 None:intergenic 45.0%
! AGCACTGAAATCAGGTTCTG+AGG - chr4.3:88181580-88181599 None:intergenic 45.0%
! ATTTTCTTCATCGACCCCTC+GGG - chr4.3:88180699-88180718 None:intergenic 45.0%
! CAATGCCATCTAGCTCAAGT+GGG + chr4.3:88178376-88178395 MS.gene65401:CDS 45.0%
! GACGAACCGACATTGATTGA+AGG - chr4.3:88180562-88180581 None:intergenic 45.0%
! GCACTGAAATCAGGTTCTGA+GGG - chr4.3:88181579-88181598 None:intergenic 45.0%
! TCAATGCCATCTAGCTCAAG+TGG + chr4.3:88178375-88178394 MS.gene65401:CDS 45.0%
! TCGAGATGAAGGTGCAATGA+AGG - chr4.3:88178817-88178836 None:intergenic 45.0%
! TTTGACGAACTTTGAGGACC+CGG - chr4.3:88181453-88181472 None:intergenic 45.0%
!! AAATCAGGTTCTGAGGGCTT+TGG - chr4.3:88181573-88181592 None:intergenic 45.0%
!! AATCAGGTTCTGAGGGCTTT+GGG - chr4.3:88181572-88181591 None:intergenic 45.0%
!! ACTACAGATGGTGGAAGAAG+TGG + chr4.3:88181060-88181079 MS.gene65401:CDS 45.0%
!! CTGCATGGAGGAAGCATTTA+AGG + chr4.3:88181349-88181368 MS.gene65401:CDS 45.0%
!! GAGAGTGTTACTACAGATGG+TGG + chr4.3:88181051-88181070 MS.gene65401:CDS 45.0%
!! GCTAGATGGCATTGACGAAT+TGG - chr4.3:88178370-88178389 None:intergenic 45.0%
!! GGCATTTGCTTTAGAAGCAG+AGG + chr4.3:88181215-88181234 MS.gene65401:CDS 45.0%
!! TCAATCAATGTCGGTTCGTC+TGG + chr4.3:88180562-88180581 MS.gene65401:CDS 45.0%
!! TCTTAACTCTCTGGTTCTCG+GGG + chr4.3:88178044-88178063 MS.gene65401:intron 45.0%
!! TGTAGATAACGGGTTCTGTG+TGG + chr4.3:88179648-88179667 MS.gene65401:intron 45.0%
!!! AATTGAATATAAATAAATTT+TGG - chr4.3:88179497-88179516 None:intergenic 5.0%
ACAAGTGGTTGAACACCCGA+GGG + chr4.3:88180681-88180700 MS.gene65401:CDS 50.0%
AGCGTCTGACTCTTTCCCTT+AGG - chr4.3:88180652-88180671 None:intergenic 50.0%
ATCCAGGTTCGGATCTTGAC+TGG - chr4.3:88178134-88178153 None:intergenic 50.0%
CGGGAAGTGATGATTCTGCA+AGG + chr4.3:88181418-88181437 MS.gene65401:CDS 50.0%
CTGACTCTTTCCCTTAGGTC+CGG - chr4.3:88180647-88180666 None:intergenic 50.0%
CTTCTGACTAGTACCTACCC+TGG + chr4.3:88180363-88180382 MS.gene65401:CDS 50.0%
GACGCTACGACCAATACAAG+TGG + chr4.3:88180666-88180685 MS.gene65401:CDS 50.0%
GTATCTCAAACCTGTGGCAG+TGG - chr4.3:88178933-88178952 None:intergenic 50.0%
TACAAGTGGTTGAACACCCG+AGG + chr4.3:88180680-88180699 MS.gene65401:CDS 50.0%
TTATAGCGCAAGACGCATGG+AGG + chr4.3:88179062-88179081 MS.gene65401:CDS 50.0%
TTCCAGTCAAGATCCGAACC+TGG + chr4.3:88178129-88178148 MS.gene65401:intron 50.0%
! AGCATTTAAGGCGAAGGCGA+AGG + chr4.3:88181361-88181380 MS.gene65401:CDS 50.0%
! TGATGATTCTGCAAGGGCGT+TGG + chr4.3:88181425-88181444 MS.gene65401:CDS 50.0%
! TTTAAGGCGAAGGCGAAGGA+AGG + chr4.3:88181365-88181384 MS.gene65401:CDS 50.0%
!! ATCAATGTCGGTTCGTCTGG+AGG + chr4.3:88180565-88180584 MS.gene65401:CDS 50.0%
!! CGGTTTGCTGCTAATCTTCG+TGG + chr4.3:88181504-88181523 MS.gene65401:CDS 50.0%
!! CTTAACTCTCTGGTTCTCGG+GGG + chr4.3:88178045-88178064 MS.gene65401:intron 50.0%
!! GGAGGAAGCATTTAAGGCGA+AGG + chr4.3:88181355-88181374 MS.gene65401:CDS 50.0%
!! TTAACTCTCTGGTTCTCGGG+GGG + chr4.3:88178046-88178065 MS.gene65401:intron 50.0%
!!! CGAATTTTTTTTCCCGCGGC+CGG - chr4.3:88178448-88178467 None:intergenic 50.0%
!!! GAATTTTTTTTCCCGCGGCC+GGG - chr4.3:88178447-88178466 None:intergenic 50.0%
!!! GGCAAGCTTGCTGCAGATTT+TGG - chr4.3:88181156-88181175 None:intergenic 50.0%
AAGGTGCAATGAAGGCGGTG+CGG - chr4.3:88178809-88178828 None:intergenic 55.0%
ACTCTTTCCCTTAGGTCCGG+AGG - chr4.3:88180644-88180663 None:intergenic 55.0%
CAAGTGGTTGAACACCCGAG+GGG + chr4.3:88180682-88180701 MS.gene65401:CDS 55.0%
CAATGAAGGCGGTGCGGAAT+TGG - chr4.3:88178803-88178822 None:intergenic 55.0%
CGGTTCGTCTGGAGGAAATG+AGG + chr4.3:88180573-88180592 MS.gene65401:CDS 55.0%
CTTGCTTCCTCCGGACCTAA+GGG + chr4.3:88180634-88180653 MS.gene65401:CDS 55.0%
GACTAGTACCTACCCTGGAG+AGG + chr4.3:88180368-88180387 MS.gene65401:CDS 55.0%
GCATCATCGCTTGCTTCCTC+CGG + chr4.3:88180625-88180644 MS.gene65401:CDS 55.0%
GCGATGATGCATCCACTGCT+CGG - chr4.3:88180615-88180634 None:intergenic 55.0%
TGGGCACATGCACCTCTACA+TGG - chr4.3:88180113-88180132 None:intergenic 55.0%
! CGCAAGACGCATGGAGGTAT+TGG + chr4.3:88179068-88179087 MS.gene65401:CDS 55.0%
!! TTCTGCAAGGGCGTTGGTTC+CGG + chr4.3:88181431-88181450 MS.gene65401:CDS 55.0%
AAGGCGAAGGCGAAGGAAGG+AGG + chr4.3:88181368-88181387 MS.gene65401:CDS 60.0%
GCTTGCTTCCTCCGGACCTA+AGG + chr4.3:88180633-88180652 MS.gene65401:CDS 60.0%
GTCTGTACTGCCACTGCCAC+AGG + chr4.3:88178920-88178939 MS.gene65401:CDS 60.0%
! AAAGGCGTGACTCCGAGCAG+TGG + chr4.3:88180600-88180619 MS.gene65401:CDS 60.0%
! CCTTTTCCTATTCCCGGCCG+CGG + chr4.3:88178432-88178451 MS.gene65401:CDS 60.0%
! CTTTTCCTATTCCCGGCCGC+GGG + chr4.3:88178433-88178452 MS.gene65401:CDS 60.0%
! TTTTTCCCGCGGCCGGGAAT+AGG - chr4.3:88178441-88178460 None:intergenic 60.0%
!! TCTGCAAGGGCGTTGGTTCC+GGG + chr4.3:88181432-88181451 MS.gene65401:CDS 60.0%
CCGCGGCCGGGAATAGGAAA+AGG - chr4.3:88178435-88178454 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 88177924 88181617 88177924 ID=MS.gene65401
chr4.3 mRNA 88177924 88181617 88177924 ID=MS.gene65401.t1;Parent=MS.gene65401
chr4.3 exon 88177924 88177982 88177924 ID=MS.gene65401.t1.exon1;Parent=MS.gene65401.t1
chr4.3 CDS 88177924 88177982 88177924 ID=cds.MS.gene65401.t1;Parent=MS.gene65401.t1
chr4.3 exon 88178350 88178941 88178350 ID=MS.gene65401.t1.exon2;Parent=MS.gene65401.t1
chr4.3 CDS 88178350 88178941 88178350 ID=cds.MS.gene65401.t1;Parent=MS.gene65401.t1
chr4.3 exon 88179042 88179254 88179042 ID=MS.gene65401.t1.exon3;Parent=MS.gene65401.t1
chr4.3 CDS 88179042 88179254 88179042 ID=cds.MS.gene65401.t1;Parent=MS.gene65401.t1
chr4.3 exon 88179653 88179963 88179653 ID=MS.gene65401.t1.exon4;Parent=MS.gene65401.t1
chr4.3 CDS 88179653 88179963 88179653 ID=cds.MS.gene65401.t1;Parent=MS.gene65401.t1
chr4.3 exon 88180347 88180458 88180347 ID=MS.gene65401.t1.exon5;Parent=MS.gene65401.t1
chr4.3 CDS 88180347 88180458 88180347 ID=cds.MS.gene65401.t1;Parent=MS.gene65401.t1
chr4.3 exon 88180554 88180790 88180554 ID=MS.gene65401.t1.exon6;Parent=MS.gene65401.t1
chr4.3 CDS 88180554 88180790 88180554 ID=cds.MS.gene65401.t1;Parent=MS.gene65401.t1
chr4.3 exon 88180907 88181617 88180907 ID=MS.gene65401.t1.exon7;Parent=MS.gene65401.t1
chr4.3 CDS 88180907 88181617 88180907 ID=cds.MS.gene65401.t1;Parent=MS.gene65401.t1
Gene Sequence

>MS.gene65401

ATGTTTGACTCTGGTTACAAGTCACAGTATATTGAAGGTCAGAAGGAGAAATTTGTGAGGTATTTTTATTTTTGGGTATAACATATCAATGTGATTGGTTACTCTTTTTTTTTTCCGTTATCTTAACTCTCTGGTTCTCGGGGGGAAAGAGACTCTATTAATCTGGATTTTGGTCAGGATGTAAGTAAAGTTTGATCAATGATTGTTCCAGTCAAGATCCGAACCTGGATATACTCTCGATAGATTTGTCATTAACGGAAGTTCATTAACCACTTGGTTCTATTGGTTAATTTAAGAACATGGACTAAATTTTGATTTTACTAGCATTTTTTTTTGTTTGTTATATTCCACTTTAAGTTTAGGGTATGCAATGTCCAATTTGTTAACATAAGCATCTTTCAGACTAATATACAGTGGATGATGTAGGTTGGATGATTTGGATTCCAATTCGTCAATGCCATCTAGCTCAAGTGGGATGAAGAAGTTAAGATTTAATTTAGATTGTTTGCCTTTTCCTATTCCCGGCCGCGGGAAAAAAAATTCGTCGAAATCATTTCGAGTCGGAGTGAAAAAGGGATCAGATGGACTAATGAATATTGGAAGATCACTCAAAAGTGGAGTCACTTGGGGAGTTTTTCCAGAAGATCTTAAAGTGTCACAGAAAAAAGTATTTGATCCTCAAGATAAGAATCTTCTGTATTGGAACAAGTTTTTCGAGATTCTATGCATTCTTTCTGTAGCTTGTGATCCTTTCTTTTTCTATCTTCCGTATTTCAACCACAAATCGTATTGTTTAGCCATAGATAACAAGCTAGCAAGTTTTACTGTTACATTGAGAACAATATGTGATTGTATTTATCTCATTCGCATAAGTTTCCAATTCCGCACCGCCTTCATTGCACCTTCATCTCGAGTATTTGGAAGAGGTGAACTTGTCATAGATCCTGGATTGATCGCAAAGCGATATTTACAACGATATTTCATTATTGACTTCTGGTCTGTACTGCCACTGCCACAGGTTTGAGATACCATCCATGCTCAATATGTTTTGCGTACTAAGTAATGTCTCATCTCTATTTTCTTTTTTTTTTGCCAATATGTCTTAATGTTTGTTGTAGATTGTAGTGTGGAGGTATCTTTATAGCGCAAGACGCATGGAGGTATTGGATACAAAAACATCACTATTGAGACATGTTATCATGCAATATTTTCCGAGAATGGTACGGTTCATACCTTTGGCTTCAGAAGTTAAAAAGACAGCTGGTGTTTTTTCTGAAAATGCATTGCTTGGTGCCATGTACTATTTGATCTGGTATATGCTTGCTAGCCATGTAAGTTCATTTCTTAAGACAATTCTTCTGTTTTAATTCTCCATTGCTTCTGTATCACTTATGAAGCTCGGATACTCCATAAAAGAAAAAATGTTGGTGTCGTATCTGTATCACTATCATTCACATATTCTCTTTGGATATTTCTCGATACGTGTCCAAAAAGCATCCAATTTTTCTATGGAAACTTCCCATTAAAATTGATACTCCTCATACACTTCTTGATACTTCCCGATTGGACACCAAAATTTATTTATATTCAATTTTTTGAGTTTTAATTATGATGCTTTTGTAGATATTCTGACATAAAGGTTAGAATAGATTACGCTATAGGCGGAAGCTCTAGCATTCTTGTTACCGACACGAATGATCAATGTTTCTTTCTAACTATTATTTTGTAGATAACGGGTTCTGTGTGGTACTTGCTAGCGATAGAACGTAACGACACATGCTGGAAGAAAGCATGTAAAGAAGTTGAGGGATGTAATTCACATTTCTTGTATTGTGGAAGTTCAAGTAAGCATATACCAGGGTATGACAGTTGGAGAAATGTCAGTCAATCTGTGCTCAAAAGTAAATGCTTTGTTGAAGATGATAACTCAGCTTTTAACTATGGAATCTTTTCTCAAGCTATAGAATCTCATATTGTTGCTTCCATAGAAGTTTTCCCCAAATTTTGTTACTGTCTATGGTGGGGACTTCAGAATTTAAGGTATGTTCTTTCTATGATTCTTTAGAACTATATATTATCCACATGAGTCCTCTTTATAACACCTATAAAACACTCAGTTTATAAATGTAATTATAATTGATAAATATATATGTATTAGTGTCGGTGTCCTATGTTTTTCACATTAGCCATGTAGAGGTGCATGTGCCCATATCGTGTGTCTGATCTTCATATCAAAGTCGGTGCTTCATATGTCTTAAATCTATAATTTATAGAATTTTAGATTGTAGATTCACTTGATGTTGGGTTAACCTATATCATCCTTTATAAATATGCTTTGATTCTTATTGATTCAAAATATGAACACATAAGATGATAACATGTTCACACTTTGACCAAATATCCTTCTGTTTTGCTTTTCACAGTACACTTGGTCAGGGGCTTCTGACTAGTACCTACCCTGGAGAGGTTTTGTTTTCCATAGTAATAGCTATTATGGGGCTTGTGCTCTTTGCTCTTCTGATTGGAAACATGCAGGTAAACCGAAAGTTTTTACTGTAAACATTGATTTATTGCATCTTGATTACTATTATACATACTTTAATCCTTGTGTTATAAATGCAATTGGACAGACCTACCTTCAATCAATGTCGGTTCGTCTGGAGGAAATGAGGATCAAAAGGCGTGACTCCGAGCAGTGGATGCATCATCGCTTGCTTCCTCCGGACCTAAGGGAAAGAGTCAGACGCTACGACCAATACAAGTGGTTGAACACCCGAGGGGTCGATGAAGAAAATTTGGTTCAGAGTCTTCCAAAAGATCTTAGAAGAGATATCAAACGTCATCTTTGTTTGAATTTAGTCAGAAGGGTGAGTACTGAGCAAACTAATTTGTCCTTTCTGAGATTTCTCATTGCAACTGAAAAGTGTTCCTTCTAACTCTTTATTTTCTCATTAGCATTCTGTGTATGACATATATTATTCAGGTTCCTCTCTTTGCTAACATGGACGAACGCTTGCTTGACGCGATATGTGAGCGATTGAAACCGAGTTTATATACAGAGAAGACTTACATAGTTAGGGAAGGTGATCCAGTTGATGAGATGTTGTTCATCATTCGTGGTCGTCTAGAGAGTGTTACTACAGATGGTGGAAGAAGTGGCTTCTTCAACAGAGGAGTTTTGAAGGAAGGTGATTTTTGTGGTGAAGAGTTGCTAACATGGGCATTGGATCCAAAATCTGCAGCAAGCTTGCCATCATCAACAAGAACCGTGAAAGCTATGAGTGAGGTAGAGGCATTTGCTTTAGAAGCAGAGGAATTGAAGTTTGTTGCATCACAGTTTAGACACATACATAGTAGACAGGTTCAGCATACATTCCGTTTCTATTCGCAACAATGGAGAACATGGGCTGCAATCTATATTCAAGCTGCATGGAGGAAGCATTTAAGGCGAAGGCGAAGGAAGGAGGAAGAAGAATATTACGAATCTGATTACCCGGGAAGTGATGATTCTGCAAGGGCGTTGGTTCCGGGTCCTCAAAGTTCGTCAAAATTTGGACTTAATACAACAGTCTATGCTTCTCGGTTTGCTGCTAATCTTCGTGGTCATAGACTTCGAGTACCAAGTTCAAGAGAGCTGGTGAATATCCCAAAGCCCTCAGAACCTGATTTCAGTGCTCTCAATGACGAAGAGTGA
Protein sequence

>MS.gene65401.t1

MFDSGYKSQYIEGQKEKFVRLDDLDSNSSMPSSSSGMKKLRFNLDCLPFPIPGRGKKNSSKSFRVGVKKGSDGLMNIGRSLKSGVTWGVFPEDLKVSQKKVFDPQDKNLLYWNKFFEILCILSVACDPFFFYLPYFNHKSYCLAIDNKLASFTVTLRTICDCIYLIRISFQFRTAFIAPSSRVFGRGELVIDPGLIAKRYLQRYFIIDFWSVLPLPQIVVWRYLYSARRMEVLDTKTSLLRHVIMQYFPRMVRFIPLASEVKKTAGVFSENALLGAMYYLIWYMLASHITGSVWYLLAIERNDTCWKKACKEVEGCNSHFLYCGSSSKHIPGYDSWRNVSQSVLKSKCFVEDDNSAFNYGIFSQAIESHIVASIEVFPKFCYCLWWGLQNLSTLGQGLLTSTYPGEVLFSIVIAIMGLVLFALLIGNMQTYLQSMSVRLEEMRIKRRDSEQWMHHRLLPPDLRERVRRYDQYKWLNTRGVDEENLVQSLPKDLRRDIKRHLCLNLVRRVPLFANMDERLLDAICERLKPSLYTEKTYIVREGDPVDEMLFIIRGRLESVTTDGGRSGFFNRGVLKEGDFCGEELLTWALDPKSAASLPSSTRTVKAMSEVEAFALEAEELKFVASQFRHIHSRQVQHTFRFYSQQWRTWAAIYIQAAWRKHLRRRRRKEEEEYYESDYPGSDDSARALVPGPQSSSKFGLNTTVYASRFAANLRGHRLRVPSSRELVNIPKPSEPDFSALNDEE