Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65997.t1 | XP_003628845.1 | 98.3 | 60 | 1 | 0 | 2 | 61 | 73 | 132 | 6.90E-25 | 122.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65997.t1 | Q9LSK9 | 55.0 | 60 | 25 | 1 | 3 | 60 | 77 | 136 | 5.7e-12 | 70.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65997.t1 | G7LHT7 | 98.3 | 60 | 1 | 0 | 2 | 61 | 73 | 132 | 5.0e-25 | 122.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053421 | MS.gene65997 | 0.808417 | 3.13E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65997.t1 | MTR_8g067590 | 98.333 | 60 | 1 | 0 | 2 | 61 | 73 | 132 | 1.46e-37 | 121 |
| MS.gene65997.t1 | MTR_5g030740 | 65.854 | 41 | 14 | 0 | 6 | 46 | 84 | 124 | 1.84e-15 | 65.5 |
| MS.gene65997.t1 | MTR_7g079650 | 58.140 | 43 | 17 | 1 | 6 | 47 | 4 | 46 | 1.32e-12 | 55.8 |
| MS.gene65997.t1 | MTR_5g067320 | 54.762 | 42 | 19 | 0 | 4 | 45 | 77 | 118 | 6.81e-11 | 53.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65997.t1 | AT5G65660 | 55.000 | 60 | 25 | 1 | 3 | 60 | 77 | 136 | 6.90e-16 | 66.6 |
Find 17 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTAGGAATTGAAGCAAAAC+AGG | 0.315810 | 8.1:-37160698 | MS.gene65997:intron |
| CATGGCATCGCTATGAACTT+TGG | 0.435341 | 8.1:+37160636 | None:intergenic |
| TGCGGTGGCTTTAGCGGTGG+CGG | 0.472991 | 8.1:+37160561 | None:intergenic |
| ATTTACAAATACAAAGGAAT+CGG | 0.482953 | 8.1:+37160534 | None:intergenic |
| GGTAGTTGCGGTGGCTTTAG+CGG | 0.501118 | 8.1:+37160555 | None:intergenic |
| GGTGGCTTTAGCGGTGGCGG+CGG | 0.535647 | 8.1:+37160564 | None:intergenic |
| AACTTTGGCACATCATCCCC+TGG | 0.540201 | 8.1:+37160651 | None:intergenic |
| TACAAAGGAATCGGTAGTTG+CGG | 0.587455 | 8.1:+37160543 | None:intergenic |
| CTAATTATTTACAAATACAA+AGG | 0.591127 | 8.1:+37160528 | None:intergenic |
| CGTGATGGTTGACACGGACA+TGG | 0.592416 | 8.1:+37160618 | None:intergenic |
| TCCGTGTCAACCATCACGGC+CGG | 0.605730 | 8.1:-37160613 | MS.gene65997:CDS |
| TCCGGCCGTGATGGTTGACA+CGG | 0.606741 | 8.1:+37160612 | None:intergenic |
| AAAGGAATCGGTAGTTGCGG+TGG | 0.608462 | 8.1:+37160546 | None:intergenic |
| TTAGGAATTGAAGCAAAACA+GGG | 0.610121 | 8.1:-37160697 | MS.gene65997:intron |
| AGTTGCGGTGGCTTTAGCGG+TGG | 0.620561 | 8.1:+37160558 | None:intergenic |
| CATGTCCGTGTCAACCATCA+CGG | 0.638623 | 8.1:-37160617 | MS.gene65997:CDS |
| TAGGAATTGAAGCAAAACAG+GGG | 0.718696 | 8.1:-37160696 | MS.gene65997:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACTAGTGTGAAAATTAAA+AGG | + | chr8.1:37160829-37160848 | None:intergenic | 20.0% |
| !! | AACTAGTGTGAAAATTAAAA+GGG | + | chr8.1:37160828-37160847 | None:intergenic | 20.0% |
| !! | AGAAAAAAGTCTAAGAATAA+TGG | + | chr8.1:37160858-37160877 | None:intergenic | 20.0% |
| ! | AAAAATGATGATCAAGAGAA+GGG | + | chr8.1:37160668-37160687 | None:intergenic | 25.0% |
| ! | ACAACAAAAACAAAACACAA+TGG | - | chr8.1:37160570-37160589 | MS.gene65997:CDS | 25.0% |
| ! | GTTATTTAATATACGTACAG+TGG | + | chr8.1:37160806-37160825 | None:intergenic | 25.0% |
| ! | TAAAAATGATGATCAAGAGA+AGG | + | chr8.1:37160669-37160688 | None:intergenic | 25.0% |
| ! | TATTTGATGAATGACTTTGA+GGG | + | chr8.1:37160768-37160787 | None:intergenic | 25.0% |
| ! | TTATTTGATGAATGACTTTG+AGG | + | chr8.1:37160769-37160788 | None:intergenic | 25.0% |
| !!! | TAGTTTCTAATGTTGTTTCA+TGG | - | chr8.1:37161055-37161074 | MS.gene65997:intron | 25.0% |
| ATTAGGAATTGAAGCAAAAC+AGG | - | chr8.1:37161082-37161101 | MS.gene65997:intron | 30.0% | |
| TTAGGAATTGAAGCAAAACA+GGG | - | chr8.1:37161083-37161102 | MS.gene65997:intron | 30.0% | |
| !! | TGTTGTTTCATGGATTGATT+AGG | - | chr8.1:37161065-37161084 | MS.gene65997:intron | 30.0% |
| !!! | ATCATCATTTTTAGCTTGAG+TGG | - | chr8.1:37160676-37160695 | MS.gene65997:CDS | 30.0% |
| ATCTGAAAAAGATTGGCGTA+AGG | + | chr8.1:37160740-37160759 | None:intergenic | 35.0% | |
| GGTCAGAATCTGAAAAAGAT+TGG | + | chr8.1:37160747-37160766 | None:intergenic | 35.0% | |
| TAGGAATTGAAGCAAAACAG+GGG | - | chr8.1:37161084-37161103 | MS.gene65997:intron | 35.0% | |
| ! | ACAGTCACAACAATGTTTTC+CGG | + | chr8.1:37161189-37161208 | None:intergenic | 35.0% |
| ! | TATCCTCTGCATTTGTTACA+CGG | - | chr8.1:37160922-37160941 | MS.gene65997:intron | 35.0% |
| ACTGGTGTAATATCAACTGC+AGG | + | chr8.1:37160993-37161012 | None:intergenic | 40.0% | |
| CTGGTGTAATATCAACTGCA+GGG | + | chr8.1:37160992-37161011 | None:intergenic | 40.0% | |
| TACAAAGGAATCGGTAGTTG+CGG | + | chr8.1:37161240-37161259 | None:intergenic | 40.0% | |
| !! | AGTTTTTCAGTGATGATGCC+AGG | - | chr8.1:37161111-37161130 | MS.gene65997:intron | 40.0% |
| !! | GTTTTTCAGTGATGATGCCA+GGG | - | chr8.1:37161112-37161131 | MS.gene65997:intron | 40.0% |
| !! | TTTTTCAGTGATGATGCCAG+GGG | - | chr8.1:37161113-37161132 | MS.gene65997:intron | 40.0% |
| ACACCGTGTAACAAATGCAG+AGG | + | chr8.1:37160928-37160947 | None:intergenic | 45.0% | |
| AGGAACGAACTTTGTCCCAA+TGG | + | chr8.1:37160720-37160739 | None:intergenic | 45.0% | |
| CATGGCATCGCTATGAACTT+TGG | + | chr8.1:37161147-37161166 | None:intergenic | 45.0% | |
| CCTCTCTTGTTGCTACCATT+GGG | - | chr8.1:37160702-37160721 | MS.gene65997:intron | 45.0% | |
| GGGATGGTGTAAGATGATCT+TGG | + | chr8.1:37160600-37160619 | None:intergenic | 45.0% | |
| TCCTCTCTTGTTGCTACCAT+TGG | - | chr8.1:37160701-37160720 | MS.gene65997:intron | 45.0% | |
| AAAGGAATCGGTAGTTGCGG+TGG | + | chr8.1:37161237-37161256 | None:intergenic | 50.0% | |
| AACTTTGGCACATCATCCCC+TGG | + | chr8.1:37161132-37161151 | None:intergenic | 50.0% | |
| AGTGCCCAAAGGGAAACCAA+GGG | + | chr8.1:37160644-37160663 | None:intergenic | 50.0% | |
| ATCCTCTGCATCAGTCACAC+TGG | + | chr8.1:37161011-37161030 | None:intergenic | 50.0% | |
| CATGTCCGTGTCAACCATCA+CGG | - | chr8.1:37161163-37161182 | MS.gene65997:intron | 50.0% | |
| CCCAATGGTAGCAACAAGAG+AGG | + | chr8.1:37160705-37160724 | None:intergenic | 50.0% | |
| TCATCTTACACCATCCCACG+TGG | - | chr8.1:37160603-37160622 | MS.gene65997:CDS | 50.0% | |
| ! | ACAATGTTTTCCGGCCGTGA+TGG | + | chr8.1:37161180-37161199 | None:intergenic | 50.0% |
| AAGAGAAGGGCAGTGCCCAA+AGG | + | chr8.1:37160655-37160674 | None:intergenic | 55.0% | |
| ACCGTCCCTTGGTTTCCCTT+TGG | - | chr8.1:37160636-37160655 | MS.gene65997:CDS | 55.0% | |
| ACGGTCTAGATGCATCCACG+TGG | + | chr8.1:37160621-37160640 | None:intergenic | 55.0% | |
| AGAGAAGGGCAGTGCCCAAA+GGG | + | chr8.1:37160654-37160673 | None:intergenic | 55.0% | |
| CACCAGTGTGACTGATGCAG+AGG | - | chr8.1:37161006-37161025 | MS.gene65997:intron | 55.0% | |
| CAGTGCCCAAAGGGAAACCA+AGG | + | chr8.1:37160645-37160664 | None:intergenic | 55.0% | |
| CCCAAAGGGAAACCAAGGGA+CGG | + | chr8.1:37160640-37160659 | None:intergenic | 55.0% | |
| CCGTCCCTTGGTTTCCCTTT+GGG | - | chr8.1:37160637-37160656 | MS.gene65997:CDS | 55.0% | |
| CGGTCTAGATGCATCCACGT+GGG | + | chr8.1:37160620-37160639 | None:intergenic | 55.0% | |
| CGTGATGGTTGACACGGACA+TGG | + | chr8.1:37161165-37161184 | None:intergenic | 55.0% | |
| CTAGATGCATCCACGTGGGA+TGG | + | chr8.1:37160616-37160635 | None:intergenic | 55.0% | |
| GATGCATCTAGACCGTCCCT+TGG | - | chr8.1:37160625-37160644 | MS.gene65997:CDS | 55.0% | |
| !! | GGTAGTTGCGGTGGCTTTAG+CGG | + | chr8.1:37161228-37161247 | None:intergenic | 55.0% |
| TCCGGCCGTGATGGTTGACA+CGG | + | chr8.1:37161171-37161190 | None:intergenic | 60.0% | |
| TCCGTGTCAACCATCACGGC+CGG | - | chr8.1:37161167-37161186 | MS.gene65997:intron | 60.0% | |
| !! | AGTTGCGGTGGCTTTAGCGG+TGG | + | chr8.1:37161225-37161244 | None:intergenic | 60.0% |
| !! | TGCGGTGGCTTTAGCGGTGG+CGG | + | chr8.1:37161222-37161241 | None:intergenic | 65.0% |
| !! | GGTGGCTTTAGCGGTGGCGG+CGG | + | chr8.1:37161219-37161238 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 37160536 | 37161266 | 37160536 | ID=MS.gene65997 |
| chr8.1 | mRNA | 37160536 | 37161266 | 37160536 | ID=MS.gene65997.t1;Parent=MS.gene65997 |
| chr8.1 | exon | 37161261 | 37161266 | 37161261 | ID=MS.gene65997.t1.exon1;Parent=MS.gene65997.t1 |
| chr8.1 | CDS | 37161261 | 37161266 | 37161261 | ID=cds.MS.gene65997.t1;Parent=MS.gene65997.t1 |
| chr8.1 | exon | 37160536 | 37160715 | 37160536 | ID=MS.gene65997.t1.exon2;Parent=MS.gene65997.t1 |
| chr8.1 | CDS | 37160536 | 37160715 | 37160536 | ID=cds.MS.gene65997.t1;Parent=MS.gene65997.t1 |
| Gene Sequence |
| Protein sequence |
