AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66060


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66060.t1 MTR_7g058640 93.333 195 13 0 1 195 1 195 7.43e-138 384
MS.gene66060.t1 MTR_7g058640 95.349 172 8 0 1 172 1 172 2.10e-123 347
MS.gene66060.t1 MTR_4g022570 74.359 195 50 0 1 195 1 195 4.00e-111 317
MS.gene66060.t1 MTR_1g047720 65.728 213 73 0 1 213 1 213 6.95e-111 317
MS.gene66060.t1 MTR_7g058900 70.256 195 58 0 1 195 1 195 4.86e-104 299
MS.gene66060.t1 MTR_4g083490 66.839 193 64 0 3 195 5 197 1.14e-100 291
MS.gene66060.t1 MTR_2g436870 60.563 213 84 0 1 213 1 213 2.93e-100 290
MS.gene66060.t1 MTR_2g028790 65.285 193 67 0 3 195 7 199 3.88e-97 282
MS.gene66060.t1 MTR_1g111890 47.186 231 103 2 1 212 1 231 7.59e-80 239
MS.gene66060.t1 MTR_7g058640 94.017 117 7 0 79 195 1 117 6.84e-77 227
MS.gene66060.t1 MTR_1g047710 60.902 133 51 1 56 187 3 135 3.60e-57 177
MS.gene66060.t1 MTR_4g022470 71.028 107 31 0 64 170 10 116 1.60e-52 165
MS.gene66060.t1 MTR_5g089370 36.649 191 118 3 5 194 2 190 4.02e-38 132
MS.gene66060.t1 MTR_4g052550 32.461 191 126 3 5 194 2 190 1.75e-36 127
MS.gene66060.t1 MTR_4g115390 29.524 210 141 4 6 210 3 210 4.08e-29 108
MS.gene66060.t1 MTR_4g022550 71.053 38 11 0 158 195 10 47 1.76e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66060.t1 AT2G33120 72.308 195 54 0 1 195 1 195 1.14e-107 308
MS.gene66060.t1 AT1G04760 68.500 200 63 0 1 200 1 200 1.64e-107 308
MS.gene66060.t1 AT1G04760 68.500 200 63 0 1 200 1 200 1.64e-107 308
MS.gene66060.t1 AT1G04750 71.795 195 55 0 1 195 1 195 4.39e-107 307
MS.gene66060.t1 AT2G33120 68.966 203 55 1 1 195 1 203 1.96e-104 301
MS.gene66060.t1 AT2G32670 69.231 195 60 0 1 195 66 260 4.44e-104 302
MS.gene66060.t1 AT4G15780 60.656 183 71 1 1 182 1 183 5.70e-83 244
MS.gene66060.t1 AT2G33110 60.513 195 73 1 1 195 1 191 6.34e-83 246
MS.gene66060.t1 AT3G54300 47.639 233 99 2 1 212 1 231 5.09e-79 236
MS.gene66060.t1 AT3G54300 47.639 233 99 2 1 212 1 231 5.09e-79 236
MS.gene66060.t1 AT3G54300 47.639 233 99 2 1 212 1 231 5.09e-79 236
MS.gene66060.t1 AT2G33110 63.095 168 62 0 1 168 1 168 2.94e-76 228
MS.gene66060.t1 AT1G04750 70.940 117 34 0 79 195 1 117 1.30e-58 181
MS.gene66060.t1 AT1G04750 70.940 117 34 0 79 195 1 117 1.30e-58 181
MS.gene66060.t1 AT1G04750 70.940 117 34 0 79 195 1 117 1.30e-58 181
MS.gene66060.t1 AT5G22360 33.014 209 135 4 5 211 2 207 2.68e-39 135
MS.gene66060.t1 AT5G11150 33.175 211 134 4 5 213 2 207 6.33e-39 134
MS.gene66060.t1 AT5G11150 33.175 211 134 4 5 213 2 207 9.28e-39 133
MS.gene66060.t1 AT4G32150 34.211 190 122 3 5 193 2 189 1.20e-34 122
MS.gene66060.t1 AT2G25340 32.105 190 126 3 5 193 2 189 4.26e-32 116
MS.gene66060.t1 AT2G33120 67.742 62 20 0 134 195 52 113 4.65e-22 88.2
MS.gene66060.t1 AT3G24890 45.745 94 45 2 113 200 14 107 2.60e-18 77.8

Find 41 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGCTTGAATAAAGAATT+TGG 0.138378 7.4:-44673404 MS.gene66060:CDS
CTTTGCAGGCACAAGATTTA+AGG 0.164312 7.4:-44672710 MS.gene66060:intron
GCAAGTTTGCTAACCTCTTC+TGG 0.229459 7.4:+44673237 None:intergenic
CAATCTTCACTTGACGATTA+AGG 0.242055 7.4:+44673062 None:intergenic
AGCTATTTCAGCAAAGTTTC+CGG 0.255031 7.4:+44673913 None:intergenic
TGCATATGCTCCTTCAATTT+CGG 0.281591 7.4:+44673276 None:intergenic
GGCGCGTGGCACGGTGATTC+TGG 0.320729 7.4:-44673954 MS.gene66060:CDS
TGGTTGCTGGGAGTCTTTGC+AGG 0.323885 7.4:+44673882 None:intergenic
AAAGCTTGAATAAAGAATTT+GGG 0.394970 7.4:-44673403 MS.gene66060:intron
TCCGGTGAAGTTGGTGTGCT+CGG 0.407070 7.4:+44673931 None:intergenic
AACTGTCAACCTTCGTGATC+AGG 0.421098 7.4:-44673023 MS.gene66060:intron
TTTGCAGGCACAAGATTTAA+GGG 0.423640 7.4:-44672709 MS.gene66060:intron
CAAGAAAGGCCATAGCAATA+TGG 0.430285 7.4:+44673489 None:intergenic
ATCACTTGTATACAGCTTTG+TGG 0.457723 7.4:-44673975 MS.gene66060:CDS
TATGTTGGAAAATATTGATC+AGG 0.458760 7.4:-44673178 MS.gene66060:intron
CTCCTTGATGTGATCAAGAA+AGG 0.487927 7.4:+44673475 None:intergenic
AGCTTACTGCCTTGTTGCTG+TGG 0.489435 7.4:-44673525 MS.gene66060:CDS
TCAAGTTCAAGATGTTATGT+TGG 0.490196 7.4:-44673193 MS.gene66060:CDS
AGCAAAGTTTCCGGTGAAGT+TGG 0.500993 7.4:+44673922 None:intergenic
CAGCTTTGTGGCGCGTGGCA+CGG 0.506415 7.4:-44673963 MS.gene66060:CDS
GTAATTTAGACCGAAATTGA+AGG 0.508592 7.4:-44673286 MS.gene66060:intron
GGCAAGTACTGAATCCATCA+TGG 0.518155 7.4:+44673810 None:intergenic
GCCGAGCACACCAACTTCAC+CGG 0.518367 7.4:-44673932 MS.gene66060:CDS
GGCCTTTCTTGATCACATCA+AGG 0.528346 7.4:-44673477 MS.gene66060:CDS
TTCAACTACCTCGCCCATGA+TGG 0.533919 7.4:-44673824 MS.gene66060:CDS
ATCATGGGCGAGGTAGTTGA+AGG 0.542753 7.4:+44673826 None:intergenic
ACTTGCGAAAGTAAAAGCTC+AGG 0.550493 7.4:-44673220 MS.gene66060:CDS
GTATACAGCTTTGTGGCGCG+TGG 0.567820 7.4:-44673968 MS.gene66060:CDS
TACTGAATCCATCATGGGCG+AGG 0.571090 7.4:+44673816 None:intergenic
TTTACCAAAAGATATGGTGG+AGG 0.594903 7.4:-44673449 MS.gene66060:CDS
TGCAGGCACAAGATTTAAGG+GGG 0.606935 7.4:-44672707 MS.gene66060:intron
TTGCAGGCACAAGATTTAAG+GGG 0.610788 7.4:-44672708 MS.gene66060:intron
GGCAATTAAGGAGAAAGATG+TGG 0.621585 7.4:-44672677 MS.gene66060:CDS
CAAAAGACTCCACAGCAACA+AGG 0.623781 7.4:+44673516 None:intergenic
GTAGTTGAAGGTATGACCAT+CGG 0.631667 7.4:+44673838 None:intergenic
GCAAGTACTGAATCCATCAT+GGG 0.632742 7.4:+44673811 None:intergenic
TTGTGTGGAACATCCAGAAG+AGG 0.637264 7.4:-44673250 MS.gene66060:CDS
AAATTCACTTACAACACCGA+TGG 0.653817 7.4:-44673854 MS.gene66060:CDS
CTTTCTTGATCACATCAAGG+AGG 0.668827 7.4:-44673474 MS.gene66060:CDS
GGAGCATATGCAATATTGTG+TGG 0.672159 7.4:-44673265 MS.gene66060:CDS
ATAAAAGGACCTGATCACGA+AGG 0.753478 7.4:+44673014 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATGTAATTTAGTTATTTA+TGG + chr7.4:44673768-44673787 None:intergenic 15.0%
!! TAAAAGAAAAAAATAAAACG+AGG + chr7.4:44672796-44672815 None:intergenic 15.0%
!! AAAAGCTTGAATAAAGAATT+TGG - chr7.4:44673178-44673197 MS.gene66060:intron 20.0%
!! AAAGCTTGAATAAAGAATTT+GGG - chr7.4:44673179-44673198 MS.gene66060:CDS 20.0%
!! AGGTATATATATGTCTATAT+TGG - chr7.4:44673424-44673443 MS.gene66060:CDS 20.0%
!! TTATAGTTATGTAACTGATA+TGG - chr7.4:44673838-44673857 MS.gene66060:CDS 20.0%
!! TTTATGGATGAACAAATAAA+AGG + chr7.4:44673586-44673605 None:intergenic 20.0%
!!! TCTTTTACATAATCTAATTG+TGG - chr7.4:44672809-44672828 MS.gene66060:intron 20.0%
!!! TTTTTTTTATTGTATGTGCA+TGG - chr7.4:44673274-44673293 MS.gene66060:CDS 20.0%
! CAAATCATAAATTTCCTTAG+AGG + chr7.4:44672966-44672985 None:intergenic 25.0%
! TATGTTGGAAAATATTGATC+AGG - chr7.4:44673404-44673423 MS.gene66060:CDS 25.0%
!! ATTTTTACACACACATGAAT+AGG - chr7.4:44673722-44673741 MS.gene66060:intron 25.0%
!! TTTTTACACACACATGAATA+GGG - chr7.4:44673723-44673742 MS.gene66060:intron 25.0%
!!! ATTTTATTTGTTTTGTCTGC+AGG - chr7.4:44673496-44673515 MS.gene66060:CDS 25.0%
GTAATTTAGACCGAAATTGA+AGG - chr7.4:44673296-44673315 MS.gene66060:intron 30.0%
TAACTGATATGGTTTGAATG+TGG - chr7.4:44673849-44673868 MS.gene66060:CDS 30.0%
TCAAATGCATTTACATGTAC+TGG - chr7.4:44672915-44672934 MS.gene66060:intron 30.0%
TCAAGTTCAAGATGTTATGT+TGG - chr7.4:44673389-44673408 MS.gene66060:intron 30.0%
! GAGGATTTTACCAAAAGATA+TGG - chr7.4:44673127-44673146 MS.gene66060:intron 30.0%
! GATTTTACCAAAAGATATGG+TGG - chr7.4:44673130-44673149 MS.gene66060:intron 30.0%
! GTTGTAAGTGAATTTTGTGT+TGG + chr7.4:44672723-44672742 None:intergenic 30.0%
! TTTTACACACACATGAATAG+GGG - chr7.4:44673724-44673743 MS.gene66060:intron 30.0%
!! AGTGAAGATTGATGTTTTGA+TGG - chr7.4:44673532-44673551 MS.gene66060:intron 30.0%
!! TGTACTGGTTTCATATATGT+CGG - chr7.4:44672930-44672949 MS.gene66060:intron 30.0%
AAATTCACTTACAACACCGA+TGG - chr7.4:44672728-44672747 MS.gene66060:intron 35.0%
ATCACTTGTATACAGCTTTG+TGG - chr7.4:44672607-44672626 MS.gene66060:CDS 35.0%
CAATCTTCACTTGACGATTA+AGG + chr7.4:44673523-44673542 None:intergenic 35.0%
TAGCTATGACACATGGTTTA+TGG + chr7.4:44673602-44673621 None:intergenic 35.0%
TATGTCTATATTGGATCCTC+TGG - chr7.4:44673433-44673452 MS.gene66060:CDS 35.0%
TCAATGCAATGCGATATATG+AGG - chr7.4:44672841-44672860 MS.gene66060:intron 35.0%
TGCATATGCTCCTTCAATTT+CGG + chr7.4:44673309-44673328 None:intergenic 35.0%
TTTACCAAAAGATATGGTGG+AGG - chr7.4:44673133-44673152 MS.gene66060:intron 35.0%
TTTGCAGGCACAAGATTTAA+GGG - chr7.4:44673873-44673892 MS.gene66060:CDS 35.0%
! AGCTATTTCAGCAAAGTTTC+CGG + chr7.4:44672672-44672691 None:intergenic 35.0%
! CAGTATTATGTTTTCTGCGA+TGG + chr7.4:44673653-44673672 None:intergenic 35.0%
! TTTTCCTCCACCATATCTTT+TGG + chr7.4:44673140-44673159 None:intergenic 35.0%
! TTTTGATCGCCATATTGCTA+TGG - chr7.4:44673084-44673103 MS.gene66060:intron 35.0%
!!! TGAATTTTGTGTTGGTTGCT+GGG + chr7.4:44672715-44672734 None:intergenic 35.0%
AACGATCTGTGCTTCAAAGT+CGG + chr7.4:44673461-44673480 None:intergenic 40.0%
AAGATTTAAGGGGGAATGCA+GGG - chr7.4:44673884-44673903 MS.gene66060:CDS 40.0%
ACTTGCGAAAGTAAAAGCTC+AGG - chr7.4:44673362-44673381 MS.gene66060:intron 40.0%
ATAAAAGGACCTGATCACGA+AGG + chr7.4:44673571-44673590 None:intergenic 40.0%
CAAGAAAGGCCATAGCAATA+TGG + chr7.4:44673096-44673115 None:intergenic 40.0%
CTCCTTGATGTGATCAAGAA+AGG + chr7.4:44673110-44673129 None:intergenic 40.0%
CTTTCTTGATCACATCAAGG+AGG - chr7.4:44673108-44673127 MS.gene66060:intron 40.0%
CTTTGCAGGCACAAGATTTA+AGG - chr7.4:44673872-44673891 MS.gene66060:CDS 40.0%
GGAGCATATGCAATATTGTG+TGG - chr7.4:44673317-44673336 MS.gene66060:intron 40.0%
GGCAATTAAGGAGAAAGATG+TGG - chr7.4:44673905-44673924 MS.gene66060:CDS 40.0%
TCATCACTAGCTATGACACA+TGG + chr7.4:44673609-44673628 None:intergenic 40.0%
TTGCAGGCACAAGATTTAAG+GGG - chr7.4:44673874-44673893 MS.gene66060:CDS 40.0%
! GTAGTTGAAGGTATGACCAT+CGG + chr7.4:44672747-44672766 None:intergenic 40.0%
!! GCAAGTACTGAATCCATCAT+GGG + chr7.4:44672774-44672793 None:intergenic 40.0%
!!! GTGAATTTTGTGTTGGTTGC+TGG + chr7.4:44672716-44672735 None:intergenic 40.0%
AACTGTCAACCTTCGTGATC+AGG - chr7.4:44673559-44673578 MS.gene66060:intron 45.0%
AGCAAAGTTTCCGGTGAAGT+TGG + chr7.4:44672663-44672682 None:intergenic 45.0%
CAAAAGACTCCACAGCAACA+AGG + chr7.4:44673069-44673088 None:intergenic 45.0%
CAAGATTTAAGGGGGAATGC+AGG - chr7.4:44673883-44673902 MS.gene66060:CDS 45.0%
GCAATGCGATATATGAGGCA+CGG - chr7.4:44672846-44672865 MS.gene66060:intron 45.0%
GGCCTTTCTTGATCACATCA+AGG - chr7.4:44673105-44673124 MS.gene66060:intron 45.0%
TCGGACATTGAACACCTCTA+AGG - chr7.4:44672949-44672968 MS.gene66060:intron 45.0%
TGCAGGCACAAGATTTAAGG+GGG - chr7.4:44673875-44673894 MS.gene66060:CDS 45.0%
TTGTGTGGAACATCCAGAAG+AGG - chr7.4:44673332-44673351 MS.gene66060:intron 45.0%
! TGGTTTGAATGTGGCTTTGC+AGG - chr7.4:44673858-44673877 MS.gene66060:CDS 45.0%
!! GCAAGTTTGCTAACCTCTTC+TGG + chr7.4:44673348-44673367 None:intergenic 45.0%
!! GGCAAGTACTGAATCCATCA+TGG + chr7.4:44672775-44672794 None:intergenic 45.0%
ATCATGGGCGAGGTAGTTGA+AGG + chr7.4:44672759-44672778 None:intergenic 50.0%
TACTGAATCCATCATGGGCG+AGG + chr7.4:44672769-44672788 None:intergenic 50.0%
TTCAACTACCTCGCCCATGA+TGG - chr7.4:44672758-44672777 MS.gene66060:intron 50.0%
! AGCTTACTGCCTTGTTGCTG+TGG - chr7.4:44673057-44673076 MS.gene66060:CDS 50.0%
GGGGGAATGCAGGGCAATTA+AGG - chr7.4:44673893-44673912 MS.gene66060:CDS 55.0%
TGGTTGCTGGGAGTCTTTGC+AGG + chr7.4:44672703-44672722 None:intergenic 55.0%
! GTATACAGCTTTGTGGCGCG+TGG - chr7.4:44672614-44672633 MS.gene66060:CDS 55.0%
! TGCTTCAAAGTCGGCTCCAG+AGG + chr7.4:44673452-44673471 None:intergenic 55.0%
!! TCCGGTGAAGTTGGTGTGCT+CGG + chr7.4:44672654-44672673 None:intergenic 55.0%
GCCGAGCACACCAACTTCAC+CGG - chr7.4:44672650-44672669 MS.gene66060:CDS 60.0%
! CAGCTTTGTGGCGCGTGGCA+CGG - chr7.4:44672619-44672638 MS.gene66060:CDS 65.0%
GGCGCGTGGCACGGTGATTC+TGG - chr7.4:44672628-44672647 MS.gene66060:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 44672596 44674008 44672596 ID=MS.gene66060
chr7.4 mRNA 44672596 44674008 44672596 ID=MS.gene66060.t1;Parent=MS.gene66060
chr7.4 exon 44673819 44674008 44673819 ID=MS.gene66060.t1.exon1;Parent=MS.gene66060.t1
chr7.4 CDS 44673819 44674008 44673819 ID=cds.MS.gene66060.t1;Parent=MS.gene66060.t1
chr7.4 exon 44673404 44673545 44673404 ID=MS.gene66060.t1.exon2;Parent=MS.gene66060.t1
chr7.4 CDS 44673404 44673545 44673404 ID=cds.MS.gene66060.t1;Parent=MS.gene66060.t1
chr7.4 exon 44673179 44673299 44673179 ID=MS.gene66060.t1.exon3;Parent=MS.gene66060.t1
chr7.4 CDS 44673179 44673299 44673179 ID=cds.MS.gene66060.t1;Parent=MS.gene66060.t1
chr7.4 exon 44673024 44673086 44673024 ID=MS.gene66060.t1.exon4;Parent=MS.gene66060.t1
chr7.4 CDS 44673024 44673086 44673024 ID=cds.MS.gene66060.t1;Parent=MS.gene66060.t1
chr7.4 exon 44672596 44672724 44672596 ID=MS.gene66060.t1.exon5;Parent=MS.gene66060.t1
chr7.4 CDS 44672596 44672724 44672596 ID=cds.MS.gene66060.t1;Parent=MS.gene66060.t1
Gene Sequence

>MS.gene66060

ATGGCGCAACAATCACTTGTATACAGCTTTGTGGCGCGTGGCACGGTGATTCTGGCCGAGCACACCAACTTCACCGGAAACTTTGCTGAAATAGCTTTGCAATGCCTGCAAAGACTCCCAGCAACCAACACAAAATTCACTTACAACACCGATGGTCATACCTTCAACTACCTCGCCCATGATGGATTCAGTACTTGCCTCGTTTTATTTTTTTCTTTTACATAATCTAATTGTGGAATAGCATATCAATGCAATGCGATATATGAGGCACGGACACTAGTGTTTAGATCTTGATAATAATTTAAGAAAATATAATTGATCAAATGCATTTACATGTACTGGTTTCATATATGTCGGACATTGAACACCTCTAAGGAAATTTATGATTTGATTTATATATTGTTTTCTTTTTCTTTCTAAAATTCTACTATATTATTAGACTTACATGATCTATTATCTTTAGCTTACTGCCTTGTTGCTGTGGAGTCTTTTGATCGCCATATTGCTATGGCCTTTCTTGATCACATCAAGGAGGATTTTACCAAAAGATATGGTGGAGGAAAAGCTGCAACAGCAACATCTAAAAGCTTGAATAAAGAATTTGGGTACTCAAGTTTATAACAATTTTGTGTATAGAAAATGTGTCACAAAAGTTGAATTGCATTAATTAACATGAAGTTTTTTTTATTGTATGTGCATGGTAATTTAGACCGAAATTGAAGGAGCATATGCAATATTGTGTGGAACATCCAGAAGAGGTTAGCAAACTTGCGAAAGTAAAAGCTCAGGTTTCTCAAGTTCAAGATGTTATGTTGGAAAATATTGATCAGGTATATATATGTCTATATTGGATCCTCTGGAGCCGACTTTGAAGCACAGATCGTTAGATTAAGAGAGAAAATTTTATTTGTTTTGTCTGCAGGTCCTTAATCGTCAAGTGAAGATTGATGTTTTGATGGACAAAACTGTCAACCTTCGTGATCAGGTCCTTTTATTTGTTCATCCATAAACCATGTGTCATAGCTAGTGATGAAATTTTCACAAAATCACAAGACCATCGCAGAAAACATAATACTGTGTTTATTTATGACTGTTCTAGTTCATGAATTACTTACTATATATTTATATTTTTACACACACATGAATAGGGGTCAAGTATAATCTAGTAACCATAAATAACTAAATTACATTGTCAAATTATCTTAACAGATATATACACATAATTTTAAATAAATTAGACATTTATAGTTATGTAACTGATATGGTTTGAATGTGGCTTTGCAGGCACAAGATTTAAGGGGGAATGCAGGGCAATTAAGGAGAAAGATGTGGTTTCAGAACATGAAGATAAAACTGATAGTTCTTGCTATCATAATTGTCATCATTCTAATAATTGTTCTTGTTCTTACATAG

Protein sequence

>MS.gene66060.t1

MAQQSLVYSFVARGTVILAEHTNFTGNFAEIALQCLQRLPATNTKFTYNTDGHTFNYLAHDGFTYCLVAVESFDRHIAMAFLDHIKEDFTKRYGGGKAATATSKSLNKEFGPKLKEHMQYCVEHPEEVSKLAKVKAQVSQVQDVMLENIDQVLNRQVKIDVLMDKTVNLRDQAQDLRGNAGQLRRKMWFQNMKIKLIVLAIIIVIILIIVLVLT