Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66256.t1 | RHN67353.1 | 98.8 | 336 | 4 | 0 | 1 | 336 | 58 | 393 | 4.40E-174 | 620.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66256.t1 | Q39218 | 64.9 | 342 | 102 | 4 | 4 | 336 | 59 | 391 | 5.2e-107 | 389.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66256.t1 | A0A072UX64 | 98.8 | 336 | 4 | 0 | 1 | 336 | 1 | 336 | 3.2e-174 | 620.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050680 | MS.gene66256 | 0.816218 | 6.19E-52 | -1.69E-46 |
| MS.gene050900 | MS.gene66256 | 0.828097 | 1.09E-54 | -1.69E-46 |
| MS.gene053494 | MS.gene66256 | 0.838114 | 3.49E-57 | -1.69E-46 |
| MS.gene05510 | MS.gene66256 | 0.801229 | 9.93E-49 | -1.69E-46 |
| MS.gene055701 | MS.gene66256 | 0.821373 | 4.18E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66256.t1 | MTR_3g058410 | 98.810 | 336 | 4 | 0 | 1 | 336 | 1 | 336 | 0.0 | 686 |
| MS.gene66256.t1 | MTR_5g095470 | 71.111 | 315 | 81 | 3 | 22 | 336 | 17 | 321 | 7.43e-158 | 444 |
| MS.gene66256.t1 | MTR_8g028040 | 67.790 | 267 | 86 | 0 | 70 | 336 | 32 | 298 | 1.31e-134 | 385 |
| MS.gene66256.t1 | MTR_5g023760 | 54.118 | 255 | 112 | 1 | 70 | 324 | 55 | 304 | 9.64e-96 | 286 |
| MS.gene66256.t1 | MTR_5g023760 | 54.118 | 255 | 112 | 1 | 70 | 324 | 55 | 304 | 1.05e-95 | 287 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66256.t1 | AT3G13110 | 65.217 | 345 | 102 | 4 | 1 | 336 | 56 | 391 | 3.04e-159 | 451 |
| MS.gene66256.t1 | AT1G55920 | 65.875 | 337 | 91 | 5 | 1 | 336 | 1 | 314 | 2.07e-153 | 433 |
| MS.gene66256.t1 | AT5G56760 | 66.045 | 268 | 90 | 1 | 70 | 336 | 45 | 312 | 7.98e-128 | 368 |
| MS.gene66256.t1 | AT4G35640 | 59.843 | 254 | 97 | 1 | 71 | 324 | 74 | 322 | 8.65e-97 | 291 |
| MS.gene66256.t1 | AT2G17640 | 56.299 | 254 | 106 | 1 | 71 | 324 | 37 | 285 | 1.67e-91 | 276 |
Find 0 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAATAGCTAGATAAAAT+TGG | + | 20997:82311-82330 | None:intergenic | 15.0% |
| !! | AATTTGACAAAATTGAATGA+AGG | + | 20997:82128-82147 | None:intergenic | 20.0% |
| !! | ATTCATGAAAAGATCAAAAA+GGG | + | 20997:82423-82442 | None:intergenic | 20.0% |
| !! | ATTTGACAAAATTGAATGAA+GGG | + | 20997:82127-82146 | None:intergenic | 20.0% |
| !! | TATTCATGAAAAGATCAAAA+AGG | + | 20997:82424-82443 | None:intergenic | 20.0% |
| !!! | ATTTTAGGGAATATTAAGAT+AGG | - | 20997:82857-82876 | MS.gene66256:CDS | 20.0% |
| !!! | ATTTTGCATAATGTTACATT+AGG | - | 20997:82761-82780 | MS.gene66256:CDS | 20.0% |
| !!! | TTTTCATGATTATGTGAAAA+GGG | + | 20997:82158-82177 | None:intergenic | 20.0% |
| !!! | TTTTTCATGATTATGTGAAA+AGG | + | 20997:82159-82178 | None:intergenic | 20.0% |
| ! | AAAAGATAATCCTATCATGT+TGG | - | 20997:82973-82992 | MS.gene66256:CDS | 25.0% |
| ! | ACATGTTCATATTCATGATA+AGG | - | 20997:82214-82233 | MS.gene66256:CDS | 25.0% |
| ! | ATACTTCATATATCTCTGAA+TGG | - | 20997:83023-83042 | MS.gene66256:CDS | 25.0% |
| ! | ATTCAGAGATATATGAAGTA+TGG | + | 20997:83024-83043 | None:intergenic | 25.0% |
| ! | ATTCATGATAAGGTTGAAAA+TGG | - | 20997:82224-82243 | MS.gene66256:CDS | 25.0% |
| !! | CATTGTTTGTTGAATTTCAA+AGG | - | 20997:82533-82552 | MS.gene66256:CDS | 25.0% |
| AGCACTCTTGATTCAAAATA+GGG | - | 20997:82619-82638 | MS.gene66256:CDS | 30.0% | |
| ATAGGGTAGCACATAAATTA+TGG | - | 20997:82576-82595 | MS.gene66256:CDS | 30.0% | |
| TAGCACTCTTGATTCAAAAT+AGG | - | 20997:82618-82637 | MS.gene66256:CDS | 30.0% | |
| TGAAAATTCAAGAAGAAGCA+AGG | - | 20997:82267-82286 | MS.gene66256:CDS | 30.0% | |
| TTGCATAATGTTACATTAGG+TGG | - | 20997:82764-82783 | MS.gene66256:CDS | 30.0% | |
| AAAACACCATCACCAATCTT+AGG | + | 20997:82818-82837 | None:intergenic | 35.0% | |
| AATGTTACATTAGGTGGAAC+TGG | - | 20997:82770-82789 | MS.gene66256:CDS | 35.0% | |
| CATGATTATGTGAAAAGGGT+CGG | + | 20997:82154-82173 | None:intergenic | 35.0% | |
| GAACATAGCTTATACAAGCA+GGG | + | 20997:82514-82533 | None:intergenic | 35.0% | |
| GATTCAAAATAGGGTTTCTG+AGG | - | 20997:82628-82647 | MS.gene66256:CDS | 35.0% | |
| GCACATAAATTATGGCTTCA+AGG | - | 20997:82584-82603 | MS.gene66256:CDS | 35.0% | |
| GGAATCTTATCCAACATGAT+AGG | + | 20997:82986-83005 | None:intergenic | 35.0% | |
| GGGAATATTAAGATAGGTGA+TGG | - | 20997:82863-82882 | MS.gene66256:CDS | 35.0% | |
| TGAACATAGCTTATACAAGC+AGG | + | 20997:82515-82534 | None:intergenic | 35.0% | |
| ! | ATTTTGTTGGATCATGCAAC+AGG | - | 20997:82695-82714 | MS.gene66256:CDS | 35.0% |
| ! | GTGCTGGAACATGTATTTTA+GGG | - | 20997:82843-82862 | MS.gene66256:CDS | 35.0% |
| ! | TAAGGTTGAAAATGGTGTTG+AGG | - | 20997:82232-82251 | MS.gene66256:CDS | 35.0% |
| ! | TCATGTTGATGAGGAATGTT+TGG | + | 20997:82101-82120 | None:intergenic | 35.0% |
| !! | TAAGATTCCTAGCTCTACTA+TGG | - | 20997:82997-83016 | MS.gene66256:CDS | 35.0% |
| !! | TTTAGCTTGTCAAGCTCATA+GGG | - | 20997:82559-82578 | MS.gene66256:CDS | 35.0% |
| !! | TTTGCTGTTGATATTCATCC+TGG | - | 20997:82653-82672 | MS.gene66256:CDS | 35.0% |
| !!! | ATTGGTGATGGTGTTTTGAT+TGG | - | 20997:82821-82840 | MS.gene66256:CDS | 35.0% |
| !!! | TTTTAGCTTGTCAAGCTCAT+AGG | - | 20997:82558-82577 | MS.gene66256:CDS | 35.0% |
| AGACATCCTAAGATTGGTGA+TGG | - | 20997:82809-82828 | MS.gene66256:CDS | 40.0% | |
| GGTGATAGACATCCTAAGAT+TGG | - | 20997:82803-82822 | MS.gene66256:CDS | 40.0% | |
| GGTGGAACTGGTAAAATTTG+TGG | - | 20997:82782-82801 | MS.gene66256:CDS | 40.0% | |
| TTTCCAACAGCAGTAGTTCT+TGG | + | 20997:82932-82951 | None:intergenic | 40.0% | |
| TTTCCACCAATCAACTTAGC+TGG | + | 20997:82956-82975 | None:intergenic | 40.0% | |
| ! | ATAGGTGATGGTGCTAAGAT+TGG | - | 20997:82875-82894 | MS.gene66256:CDS | 40.0% |
| ! | ATGGTGTTGAGGTTGATTTC+TGG | - | 20997:82243-82262 | MS.gene66256:CDS | 40.0% |
| ! | ATTCATCCTGGTGCTAAGAT+TGG | - | 20997:82665-82684 | MS.gene66256:CDS | 40.0% |
| ! | GATTGGTAGAGGGATTTTGT+TGG | - | 20997:82682-82701 | MS.gene66256:CDS | 40.0% |
| !! | AATCCAGCTAAGTTGATTGG+TGG | - | 20997:82950-82969 | MS.gene66256:CDS | 40.0% |
| !! | GGAAATCCAGCTAAGTTGAT+TGG | - | 20997:82947-82966 | MS.gene66256:CDS | 40.0% |
| !! | GGTGCTGGAACATGTATTTT+AGG | - | 20997:82842-82861 | MS.gene66256:CDS | 40.0% |
| CATGCAACAGGAGTTGTTGT+TGG | - | 20997:82707-82726 | MS.gene66256:CDS | 45.0% | |
| GAAGGGTCATCATGTTGATG+AGG | + | 20997:82110-82129 | None:intergenic | 45.0% | |
| GTATGGTCCATAGTAGAGCT+AGG | + | 20997:83007-83026 | None:intergenic | 45.0% | |
| ! | CTGGTGCTAAGATTGGTAGA+GGG | - | 20997:82672-82691 | MS.gene66256:CDS | 45.0% |
| ! | TGTTGTTGGTGAAACTGCTG+TGG | - | 20997:82721-82740 | MS.gene66256:CDS | 45.0% |
| !! | ATGTTTGGTTTGCGAGAAGG+AGG | + | 20997:82086-82105 | None:intergenic | 45.0% |
| !! | GGAATGTTTGGTTTGCGAGA+AGG | + | 20997:82089-82108 | None:intergenic | 45.0% |
| !! | TGCTGGTTCTGTTGTGTTGA+AGG | - | 20997:82898-82917 | MS.gene66256:CDS | 45.0% |
| !!! | GATGGTGTTTTGATTGGTGC+TGG | - | 20997:82827-82846 | MS.gene66256:CDS | 45.0% |
| CCAACAGCAGTAGTTCTTGG+TGG | + | 20997:82929-82948 | None:intergenic | 50.0% | |
| CCACCAAGAACTACTGCTGT+TGG | - | 20997:82926-82945 | MS.gene66256:CDS | 50.0% | |
| CCTCTACCAATCTTAGCACC+AGG | + | 20997:82674-82693 | None:intergenic | 50.0% | |
| ! | CCTGGTGCTAAGATTGGTAG+AGG | - | 20997:82671-82690 | MS.gene66256:CDS | 50.0% |
| ! | GATGGTGCTAAGATTGGTGC+TGG | - | 20997:82881-82900 | MS.gene66256:CDS | 50.0% |
| ! | GTTGGTGAAACTGCTGTGGT+TGG | - | 20997:82725-82744 | MS.gene66256:CDS | 50.0% |
| !! | TTGGTTTGCGAGAAGGAGGA+GGG | + | 20997:82082-82101 | None:intergenic | 50.0% |
| !! | TTTGGTTTGCGAGAAGGAGG+AGG | + | 20997:82083-82102 | None:intergenic | 50.0% |
| ! | TGGTTTGCGAGAAGGAGGAG+GGG | + | 20997:82081-82100 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 20997 | gene | 82053 | 83063 | 82053 | ID=MS.gene66256 |
| 20997 | mRNA | 82053 | 83063 | 82053 | ID=MS.gene66256.t1;Parent=MS.gene66256 |
| 20997 | exon | 82053 | 83063 | 82053 | ID=MS.gene66256.t1.exon1;Parent=MS.gene66256.t1 |
| 20997 | CDS | 82053 | 83063 | 82053 | ID=cds.MS.gene66256.t1;Parent=MS.gene66256.t1 |
| Gene Sequence |
| Protein sequence |
