AlfalfaGEDB | MS.gene66337

M. sativa cultivar XinJiangDaYe / MS.gene66337

KEGG: -
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66337.t1	XP_003620884.1	83.3	96	11	2	1	91	1	96	9.30E-34	152.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66337.t1	G7KJA4	83.3	96	11	2	1	91	1	96	6.7e-34	152.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052307	MS.gene66337	0.804373	2.23E-49	-1.69E-46
MS.gene055864	MS.gene66337	0.804983	1.66E-49	-1.69E-46
MS.gene056181	MS.gene66337	0.813594	2.36E-51	-1.69E-46
MS.gene058638	MS.gene66337	0.832575	8.74E-56	-1.69E-46
MS.gene058639	MS.gene66337	0.836089	1.15E-56	-1.69E-46
MS.gene058758	MS.gene66337	0.802351	5.85E-49	-1.69E-46
MS.gene060563	MS.gene66337	0.810202	1.30E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66337.t1	MTR_6g092140	83.333	96	11	2	1	91	1	96	9.31e-48	157
MS.gene66337.t1	MTR_6g091830	83.333	96	11	2	1	91	1	96	1.65e-47	157
MS.gene66337.t1	MTR_6g088340	66.981	106	20	3	1	91	1	106	1.00e-36	128

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 21 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGGCGTAATCGTTTAATTT+TGG	0.114594	6.4:+63178941	MS.gene66337:CDS
AGGATGAATAAGGTACTTAA+AGG	0.337066	6.4:+63178921	MS.gene66337:CDS
ATGGCAAACGAACATGGAAT+TGG	0.345392	6.4:+63178762	MS.gene66337:CDS
TTTCAAAGAAAGGATGAATA+AGG	0.348934	6.4:+63178911	MS.gene66337:CDS
TGACCAACTCGTTGTCGAAT+TGG	0.361665	6.4:-63179002	None:intergenic
CAGTTCAAAATTTCAAAGAA+AGG	0.407992	6.4:+63178901	MS.gene66337:CDS
CGAACATGGAATTGGGTTTG+TGG	0.442412	6.4:+63178770	MS.gene66337:CDS
TGATTCGATGATCATTCATC+AGG	0.445423	6.4:-63178869	None:intergenic
AATCTGTGTTTCATGGTAAG+AGG	0.457161	6.4:+63178802	MS.gene66337:CDS
TGGCAAACGAACATGGAATT+GGG	0.471607	6.4:+63178763	MS.gene66337:CDS
TTCGACAACGAGTTGGTCAT+AGG	0.491453	6.4:+63179006	MS.gene66337:CDS
GAAGTAAAATCTGTGTTTCA+TGG	0.497275	6.4:+63178795	MS.gene66337:CDS
GGATGAATAAGGTACTTAAA+GGG	0.501407	6.4:+63178922	MS.gene66337:CDS
AATTCCATGTTCGTTTGCCA+TGG	0.506447	6.4:-63178760	None:intergenic
ATCTGTGTTTCATGGTAAGA+GGG	0.519830	6.4:+63178803	MS.gene66337:CDS
GCCTCCATGGCAAACGAACA+TGG	0.527907	6.4:+63178756	None:intergenic
CAACGAGTTGGTCATAGGAA+TGG	0.576946	6.4:+63179011	MS.gene66337:CDS
CTTCCAATTCGACAACGAGT+TGG	0.607277	6.4:+63178999	MS.gene66337:CDS
ACATGGAATTGGGTTTGTGG+AGG	0.628720	6.4:+63178773	MS.gene66337:CDS
AATACGACGATGCTTCATGT+AGG	0.643363	6.4:-63178973	None:intergenic
GATTCGATGATCATTCATCA+GGG	0.648898	6.4:-63178868	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CAGTTCAAAATTTCAAAGAA+AGG	+	chr6.4:63178901-63178920	MS.gene66337:CDS	25.0%
!	TTTCAAAGAAAGGATGAATA+AGG	+	chr6.4:63178911-63178930	MS.gene66337:CDS	25.0%
!!!	CTTTCTTTGAAATTTTGAAC+TGG	-	chr6.4:63178903-63178922	None:intergenic	25.0%
	GAAGTAAAATCTGTGTTTCA+TGG	+	chr6.4:63178795-63178814	MS.gene66337:CDS	30.0%
!	AGGATGAATAAGGTACTTAA+AGG	+	chr6.4:63178921-63178940	MS.gene66337:CDS	30.0%
!	GGATGAATAAGGTACTTAAA+GGG	+	chr6.4:63178922-63178941	MS.gene66337:CDS	30.0%
	AATCTGTGTTTCATGGTAAG+AGG	+	chr6.4:63178802-63178821	MS.gene66337:CDS	35.0%
	ATCTGTGTTTCATGGTAAGA+GGG	+	chr6.4:63178803-63178822	MS.gene66337:CDS	35.0%
	GATTCGATGATCATTCATCA+GGG	-	chr6.4:63178871-63178890	None:intergenic	35.0%
	TGATTCGATGATCATTCATC+AGG	-	chr6.4:63178872-63178891	None:intergenic	35.0%
!	GAAATTTTGAACTGGAAGTC+TGG	-	chr6.4:63178895-63178914	None:intergenic	35.0%
!!	AGGGCGTAATCGTTTAATTT+TGG	+	chr6.4:63178941-63178960	MS.gene66337:CDS	35.0%
!!	GGGCGTAATCGTTTAATTTT+GGG	+	chr6.4:63178942-63178961	MS.gene66337:CDS	35.0%
	AATACGACGATGCTTCATGT+AGG	-	chr6.4:63178976-63178995	None:intergenic	40.0%
	ATGGCAAACGAACATGGAAT+TGG	+	chr6.4:63178762-63178781	MS.gene66337:CDS	40.0%
	TGGCAAACGAACATGGAATT+GGG	+	chr6.4:63178763-63178782	MS.gene66337:CDS	40.0%
	ACATGGAATTGGGTTTGTGG+AGG	+	chr6.4:63178773-63178792	MS.gene66337:CDS	45.0%
	CGAACATGGAATTGGGTTTG+TGG	+	chr6.4:63178770-63178789	MS.gene66337:CDS	45.0%
	CTTCCAATTCGACAACGAGT+TGG	+	chr6.4:63178999-63179018	MS.gene66337:CDS	45.0%
	TGACCAACTCGTTGTCGAAT+TGG	-	chr6.4:63179005-63179024	None:intergenic	45.0%
!!	CAACGAGTTGGTCATAGGAA+TGG	+	chr6.4:63179011-63179030	MS.gene66337:CDS	45.0%
!!	TTCGACAACGAGTTGGTCAT+AGG	+	chr6.4:63179006-63179025	MS.gene66337:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.4	gene	63178762	63179037	63178762	ID=MS.gene66337
chr6.4	mRNA	63178762	63179037	63178762	ID=MS.gene66337.t1;Parent=MS.gene66337
chr6.4	exon	63178762	63179037	63178762	ID=MS.gene66337.t1.exon1;Parent=MS.gene66337.t1
chr6.4	CDS	63178762	63179037	63178762	ID=cds.MS.gene66337.t1;Parent=MS.gene66337.t1

Gene Sequence	>MS.gene66337 ATGGCAAACGAACATGGAATTGGGTTTGTGGAGGAAGTAAAATCTGTGTTTCATGGTAAGAGGGAAGAGTATGAAGAGTTTATAAAAGACATAAATGCTTTTAAGACCCTGATGAATGATCATCGAATCACCAGACTTCCAGTTCAAAATTTCAAAGAAAGGATGAATAAGGTACTTAAAGGGCGTAATCGTTTAATTTTGGGATTCAACGCCTACATGAAGCATCGTCGTATTACACTTCCAATTCGACAACGAGTTGGTCATAGGAATGGTTAG
Protein sequence	>MS.gene66337.t1 MANEHGIGFVEEVKSVFHGKREEYEEFIKDINAFKTLMNDHRITRLPVQNFKERMNKVLKGRNRLILGFNAYMKHRRITLPIRQRVGHRNG