AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66372

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66372.t1 MTR_6g090290 91.972 436 35 0 1 436 1 436 0.0 801
MS.gene66372.t1 MTR_8g096990 70.709 437 123 2 1 437 1 432 0.0 630
MS.gene66372.t1 MTR_5g080960 70.159 439 124 5 1 437 1 434 0.0 619
MS.gene66372.t1 MTR_5g080890 69.932 439 125 5 1 437 1 434 0.0 619
MS.gene66372.t1 MTR_8g038280 66.819 437 144 1 1 437 1 436 0.0 585
MS.gene66372.t1 MTR_5g044770 65.217 437 146 3 1 437 1 431 0.0 568
MS.gene66372.t1 MTR_8g038250 63.205 443 130 4 1 437 1 416 0.0 556
MS.gene66372.t1 MTR_4g109760 62.700 437 125 6 1 437 1 399 1.05e-175 497
MS.gene66372.t1 MTR_8g038310 58.581 437 156 5 1 436 1 413 3.98e-175 496
MS.gene66372.t1 MTR_3g028120 50.000 422 192 9 27 437 26 439 3.26e-138 403
MS.gene66372.t1 MTR_5g038360 48.055 437 210 10 9 436 8 436 1.46e-131 386
MS.gene66372.t1 MTR_6g088760 49.403 419 195 10 6 415 7 417 1.46e-129 384
MS.gene66372.t1 MTR_2g067830 49.272 412 196 9 32 436 1 406 4.07e-121 358
MS.gene66372.t1 MTR_0024s0040 48.333 420 194 10 30 436 1 410 1.52e-120 357
MS.gene66372.t1 MTR_5g044570 44.196 448 201 11 1 436 1 411 4.81e-116 346
MS.gene66372.t1 MTR_2g105300 46.944 409 182 11 32 433 1 381 1.46e-109 328
MS.gene66372.t1 MTR_0036s0070 39.821 447 237 12 1 436 1 426 1.00e-104 317
MS.gene66372.t1 MTR_0036s0050 40.899 445 232 12 9 436 5 435 1.47e-103 315
MS.gene66372.t1 MTR_0036s0110 37.991 458 246 13 1 436 1 442 7.84e-93 287
MS.gene66372.t1 MTR_2g059490 42.588 371 199 10 75 437 47 411 1.53e-91 284
MS.gene66372.t1 MTR_5g008070 40.758 422 224 10 21 435 23 425 4.61e-91 282
MS.gene66372.t1 MTR_5g008150 40.310 387 212 10 59 436 36 412 1.29e-89 278
MS.gene66372.t1 MTR_2g059460 40.000 420 202 14 26 436 28 406 6.13e-89 276
MS.gene66372.t1 MTR_5g008110 39.037 374 212 8 70 436 51 415 6.18e-87 271
MS.gene66372.t1 MTR_3g097150 33.925 451 262 11 6 437 16 449 1.19e-75 243
MS.gene66372.t1 MTR_1g060750 34.644 407 224 11 40 436 2 376 2.29e-66 217
MS.gene66372.t1 MTR_4g045857 44.130 247 129 7 74 315 46 288 1.29e-57 191
MS.gene66372.t1 MTR_5g008090 37.377 305 172 9 141 436 20 314 1.48e-57 192
MS.gene66372.t1 MTR_1g039270 33.641 434 239 17 28 434 58 469 4.40e-56 192
MS.gene66372.t1 MTR_1g115210 33.100 429 257 11 21 434 73 486 8.39e-56 192
MS.gene66372.t1 MTR_7g117430 33.028 436 253 13 19 434 68 484 2.36e-55 191
MS.gene66372.t1 MTR_3g046300 34.021 388 233 11 68 437 79 461 6.21e-48 171
MS.gene66372.t1 MTR_1g111350 34.160 363 201 14 85 434 130 467 5.21e-46 166
MS.gene66372.t1 MTR_6g085010 32.861 353 216 10 86 434 156 491 4.18e-44 161
MS.gene66372.t1 MTR_2g028190 30.736 462 278 17 4 434 1 451 2.24e-43 158
MS.gene66372.t1 MTR_8g103880 30.851 376 237 12 66 434 96 455 1.29e-42 156
MS.gene66372.t1 MTR_5g091130 32.391 389 239 10 68 437 173 556 2.18e-42 157
MS.gene66372.t1 MTR_6g088765 39.506 243 108 6 162 403 5 209 3.01e-42 149
MS.gene66372.t1 MTR_4g007740 30.424 401 249 11 49 435 94 478 1.13e-39 149
MS.gene66372.t1 MTR_3g053710 31.319 364 223 10 85 434 116 466 1.67e-39 148
MS.gene66372.t1 MTR_2g055840 27.901 362 221 12 87 422 14 361 5.00e-32 125
MS.gene66372.t1 MTR_3g053980 30.769 364 219 9 85 434 141 485 1.91e-30 123
MS.gene66372.t1 MTR_4g008110 54.167 120 28 1 158 277 45 137 7.67e-29 110
MS.gene66372.t1 MTR_2g070430 26.216 473 293 20 2 436 3 457 2.39e-27 114
MS.gene66372.t1 MTR_2g063110 26.000 400 238 13 85 434 117 508 3.37e-26 111
MS.gene66372.t1 MTR_3g068000 26.943 386 222 13 89 434 76 441 4.30e-26 110
MS.gene66372.t1 MTR_7g062360 27.068 399 243 16 72 437 45 428 9.33e-26 108
MS.gene66372.t1 MTR_1g078240 25.849 383 232 15 85 435 68 430 2.39e-25 108
MS.gene66372.t1 MTR_7g080230 23.991 446 274 15 30 435 11 431 2.56e-25 108
MS.gene66372.t1 MTR_3g087780 26.477 457 267 20 24 437 23 453 3.43e-25 108
MS.gene66372.t1 MTR_4g095270 25.180 417 265 17 41 437 37 426 4.36e-25 108
MS.gene66372.t1 MTR_4g095270 25.180 417 265 17 41 437 37 426 4.57e-25 108
MS.gene66372.t1 MTR_8g075090 28.042 378 215 16 87 436 113 461 8.99e-24 103
MS.gene66372.t1 MTR_1g078240 25.781 384 232 16 85 435 68 431 9.51e-24 103
MS.gene66372.t1 MTR_7g105850 26.069 491 270 23 9 436 7 467 1.72e-23 102
MS.gene66372.t1 MTR_5g016260 24.397 373 237 15 85 437 87 434 3.72e-23 102
MS.gene66372.t1 MTR_5g016260 24.397 373 237 15 85 437 87 434 4.09e-23 102
MS.gene66372.t1 MTR_8g075090 27.704 379 211 17 87 434 113 459 1.32e-22 100
MS.gene66372.t1 MTR_8g094590 31.061 264 151 9 85 342 72 310 1.53e-22 97.8
MS.gene66372.t1 MTR_8g094590 29.720 286 164 10 85 358 72 332 1.58e-22 99.4
MS.gene66372.t1 MTR_3g065160 24.675 385 234 14 83 437 71 429 1.88e-22 99.4
MS.gene66372.t1 MTR_8g094590 29.720 286 164 10 85 358 72 332 2.26e-22 99.4
MS.gene66372.t1 MTR_2g084550 27.749 382 226 16 85 435 76 438 2.51e-22 99.8
MS.gene66372.t1 MTR_1g037630 27.465 426 270 17 26 435 27 429 4.97e-22 98.2
MS.gene66372.t1 MTR_8g039540 28.381 451 280 19 6 435 6 434 5.57e-22 98.2
MS.gene66372.t1 MTR_5g061690 29.111 371 211 19 78 422 89 433 6.74e-22 98.6
MS.gene66372.t1 MTR_0945s0010 38.583 127 73 3 314 437 1 125 1.35e-21 91.3
MS.gene66372.t1 MTR_7g021510 24.219 384 238 14 85 435 71 434 2.22e-21 96.7
MS.gene66372.t1 MTR_1g078240 27.129 317 187 13 85 377 68 364 3.15e-21 95.1
MS.gene66372.t1 MTR_1g061940 24.416 471 281 22 8 435 7 445 6.46e-21 95.5
MS.gene66372.t1 MTR_3g014290 28.969 359 223 14 87 435 81 417 6.48e-21 94.7
MS.gene66372.t1 MTR_4g056420 26.904 394 219 19 82 435 73 437 1.08e-20 94.7
MS.gene66372.t1 MTR_2g103340 40.000 125 30 2 21 145 34 113 3.82e-20 88.2
MS.gene66372.t1 MTR_7g102750 25.191 393 221 19 85 435 80 441 4.59e-20 92.8
MS.gene66372.t1 MTR_8g075090 28.116 345 193 15 92 409 118 434 1.02e-19 91.7
MS.gene66372.t1 MTR_3g087780 27.385 325 200 12 128 437 8 311 1.16e-19 90.9
MS.gene66372.t1 MTR_3g087780 27.385 325 200 12 128 437 44 347 1.62e-19 90.5
MS.gene66372.t1 MTR_1g029810 25.521 384 228 19 85 437 56 412 2.42e-19 90.1
MS.gene66372.t1 MTR_1g029810 25.521 384 228 19 85 437 56 412 3.57e-19 89.7
MS.gene66372.t1 MTR_6g084710 24.490 392 251 14 71 434 176 550 8.65e-19 89.4
MS.gene66372.t1 MTR_6g084710 26.246 301 194 11 71 360 176 459 1.07e-18 88.6
MS.gene66372.t1 MTR_5g022880 25.876 371 230 13 87 434 113 461 1.32e-18 88.6
MS.gene66372.t1 MTR_3g096930 27.178 287 167 13 85 357 83 341 3.72e-18 86.7
MS.gene66372.t1 MTR_5g077850 23.316 386 242 13 83 437 78 440 3.80e-18 86.7
MS.gene66372.t1 MTR_4g100990 26.179 424 233 19 78 437 66 473 9.14e-18 85.9
MS.gene66372.t1 MTR_7g102750 25.836 329 177 16 85 377 80 377 1.76e-16 81.6
MS.gene66372.t1 MTR_5g012490 26.210 496 269 25 6 435 6 470 1.94e-16 81.6
MS.gene66372.t1 MTR_7g080230 23.952 334 206 13 129 435 49 361 3.47e-16 80.5
MS.gene66372.t1 MTR_5g061690 28.956 297 174 15 138 422 23 294 4.36e-16 80.1
MS.gene66372.t1 MTR_5g077920 24.165 389 249 13 76 436 58 428 3.19e-15 77.8
MS.gene66372.t1 MTR_4g023630 23.711 388 238 14 84 437 59 422 9.70e-15 76.3
MS.gene66372.t1 MTR_4g023650 24.679 389 233 18 84 437 59 422 2.49e-14 75.1
MS.gene66372.t1 MTR_2g037710 28.037 214 125 8 227 434 15 205 3.74e-13 68.6
MS.gene66372.t1 MTR_1g093100 25.191 393 231 19 67 419 17 386 5.03e-13 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66372.t1 AT1G64830 47.470 415 210 4 25 437 23 431 1.02e-131 387
MS.gene66372.t1 AT5G33340 47.343 414 209 5 27 437 30 437 8.08e-125 369
MS.gene66372.t1 AT2G35615 43.371 445 230 9 7 435 6 444 5.13e-111 334
MS.gene66372.t1 AT1G31450 41.907 451 237 10 1 435 1 442 7.11e-105 318
MS.gene66372.t1 AT2G28010 37.727 440 216 12 5 437 6 394 4.38e-82 258
MS.gene66372.t1 AT2G28040 37.705 427 210 12 16 437 18 393 1.79e-77 246
MS.gene66372.t1 AT2G28030 41.176 357 177 9 87 437 61 390 3.09e-77 246
MS.gene66372.t1 AT2G28040 39.329 417 195 15 27 437 20 384 3.59e-77 245
MS.gene66372.t1 AT2G28220 36.574 432 217 11 15 437 17 400 7.55e-75 239
MS.gene66372.t1 AT2G03200 35.253 434 246 10 25 437 42 461 2.69e-73 238
MS.gene66372.t1 AT2G28225 36.508 378 206 8 69 437 48 400 2.52e-69 225
MS.gene66372.t1 AT1G01300 37.637 364 196 12 85 435 140 485 1.03e-56 194
MS.gene66372.t1 AT3G61820 35.294 391 227 11 55 434 108 483 3.22e-53 185
MS.gene66372.t1 AT2G23945 29.067 461 276 17 9 437 10 451 2.22e-52 182
MS.gene66372.t1 AT4G30030 33.777 376 221 16 70 434 61 419 1.86e-50 176
MS.gene66372.t1 AT3G20015 30.365 438 254 15 20 429 51 465 4.86e-48 171
MS.gene66372.t1 AT3G18490 31.591 421 242 13 23 434 117 500 2.51e-47 170
MS.gene66372.t1 AT1G25510 34.722 360 203 12 85 434 146 483 2.46e-46 167
MS.gene66372.t1 AT5G10770 33.518 361 214 9 85 435 130 474 3.04e-45 164
MS.gene66372.t1 AT4G30040 30.180 444 261 17 9 429 10 427 4.25e-45 162
MS.gene66372.t1 AT5G10760 33.756 394 227 11 57 434 89 464 4.20e-43 157
MS.gene66372.t1 AT3G25700 28.406 433 253 15 39 435 39 450 3.46e-41 152
MS.gene66372.t1 AT2G42980 30.664 437 262 17 31 437 102 527 4.10e-41 153
MS.gene66372.t1 AT1G79720 33.062 369 207 15 86 434 134 482 3.67e-39 147
MS.gene66372.t1 AT3G59080 29.337 392 240 13 72 437 155 535 9.61e-38 144
MS.gene66372.t1 AT5G36260 30.053 376 221 15 85 435 76 434 6.98e-37 141
MS.gene66372.t1 AT3G12700 26.807 429 273 15 30 435 51 461 5.46e-31 124
MS.gene66372.t1 AT2G39710 29.551 379 209 16 89 429 67 425 1.24e-30 123
MS.gene66372.t1 AT1G65240 27.321 377 227 14 85 435 72 427 1.36e-29 120
MS.gene66372.t1 AT3G02740 29.049 389 224 18 74 435 77 440 6.46e-29 119
MS.gene66372.t1 AT4G12920 26.893 383 245 13 61 434 31 387 7.64e-29 117
MS.gene66372.t1 AT3G02740 28.723 376 221 17 87 435 10 365 1.49e-28 116
MS.gene66372.t1 AT3G59080 28.424 387 214 12 72 437 155 499 1.81e-28 117
MS.gene66372.t1 AT3G54400 28.103 427 254 16 30 435 31 425 7.11e-28 115
MS.gene66372.t1 AT5G43100 24.242 396 259 15 63 437 45 420 2.79e-27 114
MS.gene66372.t1 AT5G43100 24.242 396 259 15 63 437 45 420 8.43e-27 113
MS.gene66372.t1 AT3G50050 26.829 410 248 17 55 437 55 439 1.52e-26 112
MS.gene66372.t1 AT5G45120 26.173 405 233 15 87 434 83 478 5.55e-26 110
MS.gene66372.t1 AT5G02190 28.418 373 221 15 96 434 82 442 9.58e-26 109
MS.gene66372.t1 AT1G09750 27.945 365 230 14 85 434 102 448 1.44e-24 105
MS.gene66372.t1 AT5G37540 26.385 379 240 14 81 434 74 438 1.56e-24 105
MS.gene66372.t1 AT3G52500 30.000 400 212 22 85 435 88 468 3.56e-24 104
MS.gene66372.t1 AT1G66180 25.990 404 239 18 69 437 51 429 1.05e-23 103
MS.gene66372.t1 AT3G51350 27.212 452 260 20 27 436 30 454 1.09e-21 97.8
MS.gene66372.t1 AT1G05840 24.672 381 238 12 85 435 78 439 1.69e-21 97.1
MS.gene66372.t1 AT5G22850 26.649 379 234 15 85 435 79 441 4.84e-21 95.9
MS.gene66372.t1 AT2G36670 24.737 380 238 15 85 435 98 458 8.41e-20 92.0
MS.gene66372.t1 AT2G36670 24.603 378 237 15 87 435 34 392 8.95e-20 91.7
MS.gene66372.t1 AT2G36670 24.603 378 237 15 87 435 34 392 8.95e-20 91.7
MS.gene66372.t1 AT2G36670 24.603 378 237 15 87 435 105 463 1.71e-19 91.3
MS.gene66372.t1 AT1G44130 24.257 404 249 17 67 437 23 402 1.96e-19 90.1
MS.gene66372.t1 AT4G33490 23.575 386 233 18 85 437 58 414 3.01e-19 89.7
MS.gene66372.t1 AT2G17760 27.397 365 219 16 92 434 109 449 3.31e-19 90.5
MS.gene66372.t1 AT5G07030 23.644 461 305 17 2 435 15 455 1.21e-18 88.2
MS.gene66372.t1 AT1G77480 24.021 383 236 18 85 437 65 422 1.31e-18 88.2
MS.gene66372.t1 AT1G77480 24.021 383 236 18 85 437 65 422 1.86e-18 87.8
MS.gene66372.t1 AT1G08210 24.672 381 236 15 85 435 130 489 4.64e-18 87.0
MS.gene66372.t1 AT1G08210 24.672 381 236 15 85 435 116 475 4.94e-18 86.7
MS.gene66372.t1 AT5G10080 26.987 478 268 28 11 436 7 455 5.06e-18 86.7
MS.gene66372.t1 AT1G08210 24.672 381 236 15 85 435 82 441 5.25e-18 86.7
MS.gene66372.t1 AT1G49050 21.253 367 237 13 104 436 49 397 7.70e-18 85.5
MS.gene66372.t1 AT1G49050 21.594 389 251 14 84 436 200 570 1.74e-17 85.1
MS.gene66372.t1 AT4G33490 29.947 187 109 7 85 263 55 227 2.71e-17 84.0
MS.gene66372.t1 AT3G25700 29.121 182 108 6 39 207 39 212 3.06e-16 80.1
MS.gene66372.t1 AT1G08210 24.781 343 209 14 85 399 82 403 1.95e-14 75.5
MS.gene66372.t1 AT3G42550 22.751 378 218 12 77 435 68 390 2.76e-14 74.7
MS.gene66372.t1 AT1G49050 24.046 262 173 9 84 333 200 447 5.70e-14 73.9
MS.gene66372.t1 AT4G35880 23.118 372 229 17 93 437 113 454 1.86e-13 72.8

Find 66 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTTCCTAAAACTGTTAT+AGG 0.142168 6.1:+78778856 MS.gene66372:CDS
GGAGATGCCGCAATCGTTTC+TGG 0.188883 6.1:+78779063 MS.gene66372:CDS
TGTGAATATTCTATTAATTA+TGG 0.194092 6.1:+78778760 MS.gene66372:CDS
CTTAGCTCAACAAAATTTCT+TGG 0.236063 6.1:+78779509 MS.gene66372:CDS
GGGTCGAGTAAACTCAATTT+TGG 0.266363 6.1:+78779042 MS.gene66372:CDS
ACAATACCATATGACTTAAT+TGG 0.286512 6.1:-78778568 None:intergenic
GAATTCAGCAATGGTAGATT+TGG 0.327057 6.1:+78779278 MS.gene66372:CDS
TAGGAGATTACAGTTGATTC+AGG 0.336604 6.1:-78778505 None:intergenic
CACATCTCTAATAACACAAT+TGG 0.340665 6.1:+78778948 MS.gene66372:CDS
CATAGACTGAATTGTTGATT+AGG 0.346843 6.1:-78779324 None:intergenic
GATTAAACTTAGGAGGGGTT+TGG 0.366519 6.1:-78778648 None:intergenic
TTGTAACTTGATGACTTTGA+TGG 0.396449 6.1:-78778670 None:intergenic
AGCTCTGGTGTAGTTGGCCT+TGG 0.403335 6.1:+78778916 MS.gene66372:CDS
CCTAAAACTGTTATAGGATG+TGG 0.410701 6.1:+78778862 MS.gene66372:CDS
CGCGTAAGCTCTGGTGTAGT+TGG 0.418544 6.1:+78778910 MS.gene66372:CDS
ACACAATTGGGACCGTCAAT+TGG 0.426973 6.1:+78778961 MS.gene66372:CDS
TCTAACGGTGGTGCCATCTT+CGG 0.431189 6.1:+78779483 MS.gene66372:CDS
TTAAAATGTGCGGTCACATA+AGG 0.431541 6.1:-78779381 None:intergenic
GACAAAAGAGTAGAATTTCC+AGG 0.431627 6.1:+78779168 MS.gene66372:CDS
TGGTAAATTCTCTTATTGTT+TGG 0.432097 6.1:+78778984 MS.gene66372:CDS
GCTCAACAAAATTTCTTGGT+TGG 0.437753 6.1:+78779513 MS.gene66372:CDS
ACCCCACCAATTAAGTCATA+TGG 0.439961 6.1:+78778562 MS.gene66372:CDS
TTGAGCTAAGCTTCCGAAGA+TGG 0.446795 6.1:-78779496 None:intergenic
GAAGTGACCGACACGATTGA+TGG 0.448925 6.1:-78778458 None:intergenic
TCTGGTGTAGTTGGCCTTGG+AGG 0.451277 6.1:+78778919 MS.gene66372:CDS
TCGTTTAAACGCGTAAGCTC+TGG 0.455409 6.1:+78778901 MS.gene66372:CDS
ACAATAGAAGCATTTAGTGT+TGG 0.456653 6.1:+78779144 MS.gene66372:CDS
TTCAATTTAGTATAAACATC+AGG 0.457412 6.1:-78779258 None:intergenic
GGTGTAGTTGGCCTTGGAGG+TGG 0.457901 6.1:+78778922 MS.gene66372:CDS
GGATGTGGAACTAACAATAC+AGG 0.467964 6.1:+78778877 MS.gene66372:CDS
GACTTTGATGGATTAAACTT+AGG 0.476937 6.1:-78778658 None:intergenic
AAAAGAAATACTAAAACAAA+AGG 0.486755 6.1:-78778317 None:intergenic
ATACAGGTAGTGACATTGTA+TGG 0.494388 6.1:+78778593 MS.gene66372:CDS
TCATATGGTATTGTCGATAC+AGG 0.494460 6.1:+78778577 MS.gene66372:CDS
TTTGATGGATTAAACTTAGG+AGG 0.495954 6.1:-78778655 None:intergenic
ACATCTCTAATAACACAATT+GGG 0.500209 6.1:+78778949 MS.gene66372:CDS
GAATTTCCAGGGTCCTCAAA+AGG 0.506670 6.1:+78779180 MS.gene66372:CDS
GAGAATTTACCACCAATTGA+CGG 0.523865 6.1:-78778973 None:intergenic
TCCTCAAAAGGTGTTGAAGA+AGG 0.536448 6.1:+78779192 MS.gene66372:CDS
TACCATATGACTTAATTGGT+GGG 0.543258 6.1:-78778564 None:intergenic
CAATTGTGTTATTAGAGATG+TGG 0.543750 6.1:-78778947 None:intergenic
AAATGAAATAGGACGGCCAT+TGG 0.543813 6.1:-78778839 None:intergenic
TTGATGGATTAAACTTAGGA+GGG 0.554944 6.1:-78778654 None:intergenic
AATTGTGTTATTAGAGATGT+GGG 0.556322 6.1:-78778946 None:intergenic
TACTAAATTGAATTCAGCAA+TGG 0.567220 6.1:+78779269 MS.gene66372:CDS
ATACCATATGACTTAATTGG+TGG 0.567621 6.1:-78778565 None:intergenic
ATCTGCACCTTTAAAATGTG+CGG 0.576686 6.1:-78779391 None:intergenic
CAATTGGGACCGTCAATTGG+TGG 0.579391 6.1:+78778964 MS.gene66372:CDS
TCAACTGTAATCTCCTACGA+GGG 0.579524 6.1:+78778511 MS.gene66372:CDS
AAGATGGCACCACCGTTAGA+CGG 0.582649 6.1:-78779480 None:intergenic
TGATGGATTAAACTTAGGAG+GGG 0.584520 6.1:-78778653 None:intergenic
TGGTTGTAACATTGTTCACA+AGG 0.591014 6.1:-78778628 None:intergenic
GAGATGTGGGTCCACCTCCA+AGG 0.595636 6.1:-78778933 None:intergenic
ACCATATGACTTAATTGGTG+GGG 0.597911 6.1:-78778563 None:intergenic
TATATCATGAGCTATTCAGT+TGG 0.598495 6.1:+78778538 MS.gene66372:CDS
CTTCAACCCTCATTACACTT+CGG 0.599430 6.1:-78779583 None:intergenic
ACAAAAGAGTAGAATTTCCA+GGG 0.643770 6.1:+78779169 MS.gene66372:CDS
CATGATATAATCACCCTCGT+AGG 0.657331 6.1:-78778524 None:intergenic
AATTTAGTATAAACATCAGG+TGG 0.660177 6.1:-78779255 None:intergenic
GGCAATCAATCACATTCACA+AGG 0.662936 6.1:+78778781 MS.gene66372:CDS
CTTACATTAGAATCTACCAA+TGG 0.676774 6.1:+78778823 MS.gene66372:CDS
CGTCGTTCCATCAATCGTGT+CGG 0.677525 6.1:+78778451 MS.gene66372:CDS
GATGTGGAACTAACAATACA+GGG 0.681631 6.1:+78778878 MS.gene66372:CDS
GTACTGCATTGACAACATGT+TGG 0.687626 6.1:-78778429 None:intergenic
ATCAACTGTAATCTCCTACG+AGG 0.740288 6.1:+78778510 MS.gene66372:CDS
ATTGTGACCAGAAACGATTG+CGG 0.753392 6.1:-78779070 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTAAAAAATATGTCAAT+GGG + chr6.1:78779021-78779040 MS.gene66372:CDS 10.0%
!! TATATTAAAAAATATGTCAA+TGG + chr6.1:78779020-78779039 MS.gene66372:CDS 10.0%
!! AAAAGAAATACTAAAACAAA+AGG - chr6.1:78778320-78778339 None:intergenic 15.0%
!! TATTAAAAAATATGTCAATG+GGG + chr6.1:78779022-78779041 MS.gene66372:CDS 15.0%
!! TGTGAATATTCTATTAATTA+TGG + chr6.1:78778760-78778779 MS.gene66372:CDS 15.0%
!! AACAAAAGGAAAAGAAAAAA+AGG - chr6.1:78778306-78778325 None:intergenic 20.0%
!! ACAAAAGGAAAAGAAAAAAA+GGG - chr6.1:78778305-78778324 None:intergenic 20.0%
!! TCAAATAGTAGAAAAATGAA+GGG - chr6.1:78779125-78779144 None:intergenic 20.0%
!! TTCAATTTAGTATAAACATC+AGG - chr6.1:78779261-78779280 None:intergenic 20.0%
! AAAGAAAAAAAGGGTAAGAA+AGG - chr6.1:78778296-78778315 None:intergenic 25.0%
! AATTTAGTATAAACATCAGG+TGG - chr6.1:78779258-78779277 None:intergenic 25.0%
! ACAATACCATATGACTTAAT+TGG - chr6.1:78778571-78778590 None:intergenic 25.0%
! ACATCTCTAATAACACAATT+GGG + chr6.1:78778949-78778968 MS.gene66372:CDS 25.0%
! GTCAAATAGTAGAAAAATGA+AGG - chr6.1:78779126-78779145 None:intergenic 25.0%
! TACTAAATTGAATTCAGCAA+TGG + chr6.1:78779269-78779288 MS.gene66372:CDS 25.0%
! TCATTTCCTAAAACTGTTAT+AGG + chr6.1:78778856-78778875 MS.gene66372:CDS 25.0%
! TGGTAAATTCTCTTATTGTT+TGG + chr6.1:78778984-78779003 MS.gene66372:CDS 25.0%
!! AATTGTGTTATTAGAGATGT+GGG - chr6.1:78778949-78778968 None:intergenic 25.0%
!!! ACAGTTTTAGGAAATGAAAT+AGG - chr6.1:78778853-78778872 None:intergenic 25.0%
!!! TGTTGGTATTTATTTTGTGT+AGG - chr6.1:78778415-78778434 None:intergenic 25.0%
!!! TTTCTTTTTCTCAAGTTGTA+AGG + chr6.1:78778332-78778351 MS.gene66372:CDS 25.0%
!!! TTTTAGGAAATGAAATAGGA+CGG - chr6.1:78778849-78778868 None:intergenic 25.0%
ACAAAAGAGTAGAATTTCCA+GGG + chr6.1:78779169-78779188 MS.gene66372:CDS 30.0%
ATACCATATGACTTAATTGG+TGG - chr6.1:78778568-78778587 None:intergenic 30.0%
CACATCTCTAATAACACAAT+TGG + chr6.1:78778948-78778967 MS.gene66372:CDS 30.0%
CTTACATTAGAATCTACCAA+TGG + chr6.1:78778823-78778842 MS.gene66372:CDS 30.0%
CTTAGCTCAACAAAATTTCT+TGG + chr6.1:78779509-78779528 MS.gene66372:CDS 30.0%
TACCATATGACTTAATTGGT+GGG - chr6.1:78778567-78778586 None:intergenic 30.0%
TATATCATGAGCTATTCAGT+TGG + chr6.1:78778538-78778557 MS.gene66372:CDS 30.0%
! CAATTGTGTTATTAGAGATG+TGG - chr6.1:78778950-78778969 None:intergenic 30.0%
! CATAGACTGAATTGTTGATT+AGG - chr6.1:78779327-78779346 None:intergenic 30.0%
! TTGTAACTTGATGACTTTGA+TGG - chr6.1:78778673-78778692 None:intergenic 30.0%
! TTTTGTGTAGGCTTGTAAAA+TGG - chr6.1:78778403-78778422 None:intergenic 30.0%
!! ACAATAGAAGCATTTAGTGT+TGG + chr6.1:78779144-78779163 MS.gene66372:CDS 30.0%
!! GACTTTGATGGATTAAACTT+AGG - chr6.1:78778661-78778680 None:intergenic 30.0%
!! TTGATGGATTAAACTTAGGA+GGG - chr6.1:78778657-78778676 None:intergenic 30.0%
!! TTTGATGGATTAAACTTAGG+AGG - chr6.1:78778658-78778677 None:intergenic 30.0%
!! TTTTCTCAAGTTGTAAGGAA+TGG + chr6.1:78778337-78778356 MS.gene66372:CDS 30.0%
!!! AAGACACAGTTTTTTGTTGA+AGG - chr6.1:78779545-78779564 None:intergenic 30.0%
!!! GAAGGGTCTTTTTTAACTAT+AGG - chr6.1:78779108-78779127 None:intergenic 30.0%
ACCATATGACTTAATTGGTG+GGG - chr6.1:78778566-78778585 None:intergenic 35.0%
ATACAGGTAGTGACATTGTA+TGG + chr6.1:78778593-78778612 MS.gene66372:CDS 35.0%
CCTAAAACTGTTATAGGATG+TGG + chr6.1:78778862-78778881 MS.gene66372:CDS 35.0%
GAATTCAGCAATGGTAGATT+TGG + chr6.1:78779278-78779297 MS.gene66372:CDS 35.0%
GACAAAAGAGTAGAATTTCC+AGG + chr6.1:78779168-78779187 MS.gene66372:CDS 35.0%
GAGAATTTACCACCAATTGA+CGG - chr6.1:78778976-78778995 None:intergenic 35.0%
GATGTGGAACTAACAATACA+GGG + chr6.1:78778878-78778897 MS.gene66372:CDS 35.0%
GCTCAACAAAATTTCTTGGT+TGG + chr6.1:78779513-78779532 MS.gene66372:CDS 35.0%
TCATATGGTATTGTCGATAC+AGG + chr6.1:78778577-78778596 MS.gene66372:CDS 35.0%
TGGTTGTAACATTGTTCACA+AGG - chr6.1:78778631-78778650 None:intergenic 35.0%
TTAAAATGTGCGGTCACATA+AGG - chr6.1:78779384-78779403 None:intergenic 35.0%
! CCACATCCTATAACAGTTTT+AGG - chr6.1:78778865-78778884 None:intergenic 35.0%
! TAGGAGATTACAGTTGATTC+AGG - chr6.1:78778508-78778527 None:intergenic 35.0%
! TATGTGACCGCACATTTTAA+AGG + chr6.1:78779384-78779403 MS.gene66372:CDS 35.0%
!! ATCTGCACCTTTAAAATGTG+CGG - chr6.1:78779394-78779413 None:intergenic 35.0%
!! ATGGTGATTTTAACGAGTCA+CGG - chr6.1:78778384-78778403 None:intergenic 35.0%
!! TGATGGATTAAACTTAGGAG+GGG - chr6.1:78778656-78778675 None:intergenic 35.0%
AAATGAAATAGGACGGCCAT+TGG - chr6.1:78778842-78778861 None:intergenic 40.0%
ACCCCACCAATTAAGTCATA+TGG + chr6.1:78778562-78778581 MS.gene66372:CDS 40.0%
ATCAACTGTAATCTCCTACG+AGG + chr6.1:78778510-78778529 MS.gene66372:CDS 40.0%
ATTGTGACCAGAAACGATTG+CGG - chr6.1:78779073-78779092 None:intergenic 40.0%
CATGATATAATCACCCTCGT+AGG - chr6.1:78778527-78778546 None:intergenic 40.0%
GATTAAACTTAGGAGGGGTT+TGG - chr6.1:78778651-78778670 None:intergenic 40.0%
GGATGTGGAACTAACAATAC+AGG + chr6.1:78778877-78778896 MS.gene66372:CDS 40.0%
GGCAATCAATCACATTCACA+AGG + chr6.1:78778781-78778800 MS.gene66372:CDS 40.0%
TCAACTGTAATCTCCTACGA+GGG + chr6.1:78778511-78778530 MS.gene66372:CDS 40.0%
! ACCTTCTTCAACACCTTTTG+AGG - chr6.1:78779196-78779215 None:intergenic 40.0%
! GGGTCGAGTAAACTCAATTT+TGG + chr6.1:78779042-78779061 MS.gene66372:CDS 40.0%
! GTACTGCATTGACAACATGT+TGG - chr6.1:78778432-78778451 None:intergenic 40.0%
!! TCCTCAAAAGGTGTTGAAGA+AGG + chr6.1:78779192-78779211 MS.gene66372:CDS 40.0%
!! TTTGCTTTTGCTCCGTCTAA+CGG + chr6.1:78779468-78779487 MS.gene66372:CDS 40.0%
ACACAATTGGGACCGTCAAT+TGG + chr6.1:78778961-78778980 MS.gene66372:CDS 45.0%
GAATTTCCAGGGTCCTCAAA+AGG + chr6.1:78779180-78779199 MS.gene66372:CDS 45.0%
TCGTTTAAACGCGTAAGCTC+TGG + chr6.1:78778901-78778920 MS.gene66372:CDS 45.0%
TTGAGCTAAGCTTCCGAAGA+TGG - chr6.1:78779499-78779518 None:intergenic 45.0%
AAGATGGCACCACCGTTAGA+CGG - chr6.1:78779483-78779502 None:intergenic 50.0%
CAATTGGGACCGTCAATTGG+TGG + chr6.1:78778964-78778983 MS.gene66372:CDS 50.0%
CGTCGTTCCATCAATCGTGT+CGG + chr6.1:78778451-78778470 MS.gene66372:CDS 50.0%
GAAGTGACCGACACGATTGA+TGG - chr6.1:78778461-78778480 None:intergenic 50.0%
!! TCAACACCTTTTGAGGACCC+TGG - chr6.1:78779189-78779208 None:intergenic 50.0%
!! TCTAACGGTGGTGCCATCTT+CGG + chr6.1:78779483-78779502 MS.gene66372:CDS 50.0%
GGAGATGCCGCAATCGTTTC+TGG + chr6.1:78779063-78779082 MS.gene66372:CDS 55.0%
! AGCTCTGGTGTAGTTGGCCT+TGG + chr6.1:78778916-78778935 MS.gene66372:CDS 55.0%
! CGCGTAAGCTCTGGTGTAGT+TGG + chr6.1:78778910-78778929 MS.gene66372:CDS 55.0%
! GCTTTTGCTCCGTCTAACGG+TGG + chr6.1:78779471-78779490 MS.gene66372:CDS 55.0%
!! TCTGGTGTAGTTGGCCTTGG+AGG + chr6.1:78778919-78778938 MS.gene66372:CDS 55.0%
GAGATGTGGGTCCACCTCCA+AGG - chr6.1:78778936-78778955 None:intergenic 60.0%
!! GGTGTAGTTGGCCTTGGAGG+TGG + chr6.1:78778922-78778941 MS.gene66372:CDS 60.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 78778280 78779593 78778280 ID=MS.gene66372
chr6.1 mRNA 78778280 78779593 78778280 ID=MS.gene66372.t1;Parent=MS.gene66372
chr6.1 exon 78778280 78779593 78778280 ID=MS.gene66372.t1.exon1;Parent=MS.gene66372.t1
chr6.1 CDS 78778280 78779593 78778280 ID=cds.MS.gene66372.t1;Parent=MS.gene66372.t1
Gene Sequence

>MS.gene66372

ATGAACACACTTTCCTTTCTTACCCTTTTTTTCTTTTCCTTTTGTTTTAGTATTTCTTTTTCTCAAGTTGTAAGGAATGGTTTTAGTGTTGAACTTATACACCGTGACTCGTTAAAATCACCATTTTACAAGCCTACACAAAATAAATACCAACATGTTGTCAATGCAGTACGTCGTTCCATCAATCGTGTCGGTCACTTCTACAAGAATTCTCTCGCAAGCACACCTGAATCAACTGTAATCTCCTACGAGGGTGATTATATCATGAGCTATTCAGTTGGTACCCCACCAATTAAGTCATATGGTATTGTCGATACAGGTAGTGACATTGTATGGCTACAATGCGAGCCTTGTGAACAATGTTACAACCAAACCCCTCCTAAGTTTAATCCATCAAAGTCATCAAGTTACAAAAACATTTCTTGTTCGTCTAAACTATGTCAATCTGTGAAAGATATCTCTTGCAATGACAAAAAAAATTGTGAATATTCTATTAATTATGGCAATCAATCACATTCACAAGGAGATCTTAGTGTTGAGACTCTTACATTAGAATCTACCAATGGCCGTCCTATTTCATTTCCTAAAACTGTTATAGGATGTGGAACTAACAATACAGGGTCGTTTAAACGCGTAAGCTCTGGTGTAGTTGGCCTTGGAGGTGGACCCACATCTCTAATAACACAATTGGGACCGTCAATTGGTGGTAAATTCTCTTATTGTTTGGTGCGAATGTCAATTATATTAAAAAATATGTCAATGGGGTCGAGTAAACTCAATTTTGGAGATGCCGCAATCGTTTCTGGTCACAATGTATTGTCAACTCCTATAGTTAAAAAAGACCCTTCATTTTTCTACTATTTGACAATAGAAGCATTTAGTGTTGGAGACAAAAGAGTAGAATTTCCAGGGTCCTCAAAAGGTGTTGAAGAAGGTAACATCATAATTGATTCAAGTACAATTGTGACATTTGTACCACCTGATGTTTATACTAAATTGAATTCAGCAATGGTAGATTTGGTGACACTAGAACGTGTTGATGATCCTAATCAACAATTCAGTCTATGTTATAATTCGTCGTCAGATGAAGAATATGATTTTCCTTATGTGACCGCACATTTTAAAGGTGCAGATATTTTGCTGTATGCTACTAATACGTTTATCGAAGTTGCTAGAGATGTTCTTTGCTTTGCTTTTGCTCCGTCTAACGGTGGTGCCATCTTCGGAAGCTTAGCTCAACAAAATTTCTTGGTTGGTTATGACCTTCAACAAAAAACTGTGTCTTTTAAGTCTATAGATTGTACCGAAGTGTAA
Protein sequence

>MS.gene66372.t1

MNTLSFLTLFFFSFCFSISFSQVVRNGFSVELIHRDSLKSPFYKPTQNKYQHVVNAVRRSINRVGHFYKNSLASTPESTVISYEGDYIMSYSVGTPPIKSYGIVDTGSDIVWLQCEPCEQCYNQTPPKFNPSKSSSYKNISCSSKLCQSVKDISCNDKKNCEYSINYGNQSHSQGDLSVETLTLESTNGRPISFPKTVIGCGTNNTGSFKRVSSGVVGLGGGPTSLITQLGPSIGGKFSYCLVRMSIILKNMSMGSSKLNFGDAAIVSGHNVLSTPIVKKDPSFFYYLTIEAFSVGDKRVEFPGSSKGVEEGNIIIDSSTIVTFVPPDVYTKLNSAMVDLVTLERVDDPNQQFSLCYNSSSDEEYDFPYVTAHFKGADILLYATNTFIEVARDVLCFAFAPSNGGAIFGSLAQQNFLVGYDLQQKTVSFKSIDCTEV