Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66686.t1 | KEH29787.1 | 89.1 | 248 | 22 | 1 | 1 | 243 | 1 | 248 | 6.20E-117 | 430.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66686.t1 | A0A072UJD0 | 89.1 | 248 | 22 | 1 | 1 | 243 | 1 | 248 | 4.4e-117 | 430.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059276 | MS.gene66686 | 0.818952 | 1.50E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66686.t1 | MTR_4g051655 | 89.113 | 248 | 22 | 1 | 1 | 243 | 1 | 248 | 8.93e-165 | 455 |
| MS.gene66686.t1 | MTR_4g051748 | 88.710 | 248 | 23 | 1 | 1 | 243 | 1 | 248 | 1.65e-164 | 454 |
| MS.gene66686.t1 | MTR_4g051685 | 88.646 | 229 | 21 | 1 | 1 | 224 | 1 | 229 | 8.72e-151 | 419 |
| MS.gene66686.t1 | MTR_4g051712 | 85.185 | 216 | 27 | 1 | 33 | 243 | 11 | 226 | 1.88e-135 | 380 |
| MS.gene66686.t1 | MTR_4g051675 | 85.185 | 189 | 23 | 1 | 1 | 184 | 1 | 189 | 1.49e-120 | 342 |
| MS.gene66686.t1 | MTR_4g051668 | 93.000 | 100 | 7 | 0 | 133 | 232 | 1 | 100 | 2.61e-66 | 202 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTGCGGGTTGGTGTATT+TGG | 0.211888 | 4.3:-48113473 | MS.gene66686:CDS |
| TCCACAGGTTTGTTGTAAAA+AGG | 0.227571 | 4.3:+48113571 | None:intergenic |
| ACTGCACTCTTTGCAATTCT+TGG | 0.247706 | 4.3:-48113829 | MS.gene66686:CDS |
| AACGTTAGAATTGCGTTGTT+TGG | 0.315763 | 4.3:-48113280 | MS.gene66686:CDS |
| AAAGTTGAAATGTGTAATTC+AGG | 0.335727 | 4.3:-48113364 | MS.gene66686:CDS |
| GGTGTAAAGAGAGGGTTTCT+TGG | 0.368391 | 4.3:+48113897 | None:intergenic |
| ATGGTAGTGGAGAAGCTTGC+GGG | 0.376567 | 4.3:-48113486 | MS.gene66686:CDS |
| TCAAGCTTGTCTTTCATAAC+CGG | 0.401792 | 4.3:+48113406 | None:intergenic |
| GTGCGATGTTTATAGTGATT+TGG | 0.404075 | 4.3:-48113308 | MS.gene66686:CDS |
| AGTGTGAAAGAAGGAGTTAA+AGG | 0.408012 | 4.3:+48113757 | None:intergenic |
| TCTTTGCAATTCTTGGTGAA+TGG | 0.409634 | 4.3:-48113822 | MS.gene66686:CDS |
| AAGAAATGCTCAACAATAAG+AGG | 0.421960 | 4.3:+48113220 | None:intergenic |
| ATCGATGGATGGAAACTTCA+TGG | 0.423659 | 4.3:-48113505 | MS.gene66686:CDS |
| ATAGACATGTTACGAAACTT+TGG | 0.460350 | 4.3:+48113532 | None:intergenic |
| GTCGAATGTAGTGTGAAAGA+AGG | 0.506244 | 4.3:+48113748 | None:intergenic |
| GGATGGAAACTTCATGGTAG+TGG | 0.508666 | 4.3:-48113499 | MS.gene66686:CDS |
| CTTGGGTGTCGAGTTTCTTG+CGG | 0.517467 | 4.3:-48113692 | MS.gene66686:CDS |
| AGATGAATCATCAAGATCAA+TGG | 0.525554 | 4.3:+48113876 | None:intergenic |
| GTTATGAAAGACAAGCTTGA+AGG | 0.525801 | 4.3:-48113403 | MS.gene66686:CDS |
| TCTTGAATTGAAGCATCCAC+AGG | 0.541128 | 4.3:+48113556 | None:intergenic |
| CTTTCTTCTTGTAATTGTCG+TGG | 0.546415 | 4.3:-48113248 | MS.gene66686:CDS |
| CATCGCACGACGCAAGAAGC+GGG | 0.558012 | 4.3:+48113323 | None:intergenic |
| GCGGAACACTCCAACACTAT+CGG | 0.564968 | 4.3:-48113673 | MS.gene66686:CDS |
| GACACCCAAGTTGGCTGTGA+AGG | 0.565039 | 4.3:+48113705 | None:intergenic |
| CATTCGACAACTTCAACCAA+TGG | 0.571535 | 4.3:-48113733 | MS.gene66686:CDS |
| AAAACCTTCACAGCCAACTT+GGG | 0.574055 | 4.3:-48113709 | MS.gene66686:CDS |
| AAGATCAATGGTGTAAAGAG+AGG | 0.582402 | 4.3:+48113888 | None:intergenic |
| AACATGTCTATGACGATCGA+TGG | 0.582531 | 4.3:-48113520 | MS.gene66686:CDS |
| TCTTTCATAACCGGCCACAT+TGG | 0.587722 | 4.3:+48113415 | None:intergenic |
| CATGGTAGTGGAGAAGCTTG+CGG | 0.592814 | 4.3:-48113487 | MS.gene66686:CDS |
| ATACATGCAACCAATGTGGC+CGG | 0.596773 | 4.3:-48113425 | MS.gene66686:CDS |
| GACAAGCTTGAAGGTCATTG+TGG | 0.600206 | 4.3:-48113394 | MS.gene66686:CDS |
| ACATCGCACGACGCAAGAAG+CGG | 0.601711 | 4.3:+48113322 | None:intergenic |
| TGTCTATGACGATCGATGGA+TGG | 0.605468 | 4.3:-48113516 | MS.gene66686:CDS |
| AGATCAATGGTGTAAAGAGA+GGG | 0.607118 | 4.3:+48113889 | None:intergenic |
| AAGAAACTCGACACCCAAGT+TGG | 0.612858 | 4.3:+48113696 | None:intergenic |
| AATTGTGCTTCCGATAGTGT+TGG | 0.615110 | 4.3:+48113663 | None:intergenic |
| AAAAGGCTTCGAAATAGAGA+AGG | 0.615425 | 4.3:+48113588 | None:intergenic |
| TCGAAATAGAGAAGGTTGAG+AGG | 0.624875 | 4.3:+48113596 | None:intergenic |
| CGAGATACATGCAACCAATG+TGG | 0.682707 | 4.3:-48113429 | MS.gene66686:CDS |
| TAGTGGAGAAGCTTGCGGGT+TGG | 0.710891 | 4.3:-48113482 | MS.gene66686:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGTTGAAATGTGTAATTC+AGG | - | chr4.3:48113741-48113760 | MS.gene66686:CDS | 25.0% |
| AAGAAATGCTCAACAATAAG+AGG | + | chr4.3:48113888-48113907 | None:intergenic | 30.0% | |
| AGATGAATCATCAAGATCAA+TGG | + | chr4.3:48113232-48113251 | None:intergenic | 30.0% | |
| ATAGACATGTTACGAAACTT+TGG | + | chr4.3:48113576-48113595 | None:intergenic | 30.0% | |
| AAAAGGCTTCGAAATAGAGA+AGG | + | chr4.3:48113520-48113539 | None:intergenic | 35.0% | |
| AACGTTAGAATTGCGTTGTT+TGG | - | chr4.3:48113825-48113844 | MS.gene66686:CDS | 35.0% | |
| AAGATCAATGGTGTAAAGAG+AGG | + | chr4.3:48113220-48113239 | None:intergenic | 35.0% | |
| AGATCAATGGTGTAAAGAGA+GGG | + | chr4.3:48113219-48113238 | None:intergenic | 35.0% | |
| AGTGTGAAAGAAGGAGTTAA+AGG | + | chr4.3:48113351-48113370 | None:intergenic | 35.0% | |
| CTTTCTTCTTGTAATTGTCG+TGG | - | chr4.3:48113857-48113876 | MS.gene66686:CDS | 35.0% | |
| GTGCGATGTTTATAGTGATT+TGG | - | chr4.3:48113797-48113816 | MS.gene66686:CDS | 35.0% | |
| GTTATGAAAGACAAGCTTGA+AGG | - | chr4.3:48113702-48113721 | MS.gene66686:CDS | 35.0% | |
| TCAAGCTTGTCTTTCATAAC+CGG | + | chr4.3:48113702-48113721 | None:intergenic | 35.0% | |
| TCTTTGCAATTCTTGGTGAA+TGG | - | chr4.3:48113283-48113302 | MS.gene66686:CDS | 35.0% | |
| !! | TCCACAGGTTTGTTGTAAAA+AGG | + | chr4.3:48113537-48113556 | None:intergenic | 35.0% |
| AAAAACCTTCACAGCCAACT+TGG | - | chr4.3:48113395-48113414 | MS.gene66686:CDS | 40.0% | |
| AAAACCTTCACAGCCAACTT+GGG | - | chr4.3:48113396-48113415 | MS.gene66686:CDS | 40.0% | |
| AACATGTCTATGACGATCGA+TGG | - | chr4.3:48113585-48113604 | MS.gene66686:CDS | 40.0% | |
| AATTGTGCTTCCGATAGTGT+TGG | + | chr4.3:48113445-48113464 | None:intergenic | 40.0% | |
| ACTGCACTCTTTGCAATTCT+TGG | - | chr4.3:48113276-48113295 | MS.gene66686:CDS | 40.0% | |
| ATCGATGGATGGAAACTTCA+TGG | - | chr4.3:48113600-48113619 | MS.gene66686:CDS | 40.0% | |
| CATTCGACAACTTCAACCAA+TGG | - | chr4.3:48113372-48113391 | MS.gene66686:CDS | 40.0% | |
| GTCGAATGTAGTGTGAAAGA+AGG | + | chr4.3:48113360-48113379 | None:intergenic | 40.0% | |
| TCGAAATAGAGAAGGTTGAG+AGG | + | chr4.3:48113512-48113531 | None:intergenic | 40.0% | |
| ! | AGGTTGAGAGGTTTTCTTGA+CGG | + | chr4.3:48113500-48113519 | None:intergenic | 40.0% |
| ! | GCCTTTTTACAACAAACCTG+TGG | - | chr4.3:48113533-48113552 | MS.gene66686:CDS | 40.0% |
| ! | TCTTGAATTGAAGCATCCAC+AGG | + | chr4.3:48113552-48113571 | None:intergenic | 40.0% |
| AAGAAACTCGACACCCAAGT+TGG | + | chr4.3:48113412-48113431 | None:intergenic | 45.0% | |
| ATACATGCAACCAATGTGGC+CGG | - | chr4.3:48113680-48113699 | MS.gene66686:CDS | 45.0% | |
| CGAGATACATGCAACCAATG+TGG | - | chr4.3:48113676-48113695 | MS.gene66686:CDS | 45.0% | |
| GACAAGCTTGAAGGTCATTG+TGG | - | chr4.3:48113711-48113730 | MS.gene66686:CDS | 45.0% | |
| GGATGGAAACTTCATGGTAG+TGG | - | chr4.3:48113606-48113625 | MS.gene66686:CDS | 45.0% | |
| GGTGTAAAGAGAGGGTTTCT+TGG | + | chr4.3:48113211-48113230 | None:intergenic | 45.0% | |
| TCTTTCATAACCGGCCACAT+TGG | + | chr4.3:48113693-48113712 | None:intergenic | 45.0% | |
| TGTCTATGACGATCGATGGA+TGG | - | chr4.3:48113589-48113608 | MS.gene66686:CDS | 45.0% | |
| ! | GGCTGTGAAGGTTTTTCCAT+TGG | + | chr4.3:48113391-48113410 | None:intergenic | 45.0% |
| ! | TTGAGAGGTTTTCTTGACGG+TGG | + | chr4.3:48113497-48113516 | None:intergenic | 45.0% |
| GCGGAACACTCCAACACTAT+CGG | - | chr4.3:48113432-48113451 | MS.gene66686:CDS | 50.0% | |
| !! | AGCTTGCGGGTTGGTGTATT+TGG | - | chr4.3:48113632-48113651 | MS.gene66686:CDS | 50.0% |
| !! | ATGGTAGTGGAGAAGCTTGC+GGG | - | chr4.3:48113619-48113638 | MS.gene66686:CDS | 50.0% |
| !! | CATGGTAGTGGAGAAGCTTG+CGG | - | chr4.3:48113618-48113637 | MS.gene66686:CDS | 50.0% |
| !! | CTTGGGTGTCGAGTTTCTTG+CGG | - | chr4.3:48113413-48113432 | MS.gene66686:CDS | 50.0% |
| ACATCGCACGACGCAAGAAG+CGG | + | chr4.3:48113786-48113805 | None:intergenic | 55.0% | |
| TAGTGGAGAAGCTTGCGGGT+TGG | - | chr4.3:48113623-48113642 | MS.gene66686:CDS | 55.0% | |
| ! | GACACCCAAGTTGGCTGTGA+AGG | + | chr4.3:48113403-48113422 | None:intergenic | 55.0% |
| CATCGCACGACGCAAGAAGC+GGG | + | chr4.3:48113785-48113804 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 48113198 | 48113929 | 48113198 | ID=MS.gene66686 |
| chr4.3 | mRNA | 48113198 | 48113929 | 48113198 | ID=MS.gene66686.t1;Parent=MS.gene66686 |
| chr4.3 | exon | 48113198 | 48113929 | 48113198 | ID=MS.gene66686.t1.exon1;Parent=MS.gene66686.t1 |
| chr4.3 | CDS | 48113198 | 48113929 | 48113198 | ID=cds.MS.gene66686.t1;Parent=MS.gene66686.t1 |
| Gene Sequence |
| Protein sequence |
