AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66688

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66688.t1 MTR_4g051702 92.135 267 20 1 1 266 114 380 2.95e-177 499
MS.gene66688.t1 MTR_3g014380 75.740 338 67 5 3 334 118 446 3.09e-176 498
MS.gene66688.t1 MTR_6g023380 77.778 279 52 4 50 320 1 277 3.90e-151 427
MS.gene66688.t1 MTR_8g104250 72.819 298 67 5 37 325 94 386 1.66e-140 404
MS.gene66688.t1 MTR_8g105060 69.677 310 70 4 13 312 119 414 1.06e-137 399
MS.gene66688.t1 MTR_8g105090 85.586 222 29 1 2 220 104 325 5.30e-132 379
MS.gene66688.t1 MTR_2g020660 49.175 303 133 3 12 312 133 416 5.85e-93 292
MS.gene66688.t1 MTR_7g044770 47.351 302 139 2 12 312 140 422 2.03e-91 289
MS.gene66688.t1 MTR_2g028350 47.405 289 123 4 9 296 126 386 3.70e-80 253
MS.gene66688.t1 MTR_2g029120 46.405 306 129 7 14 312 107 384 1.69e-79 250
MS.gene66688.t1 MTR_2g029120 46.405 306 129 7 14 312 107 384 2.17e-79 250
MS.gene66688.t1 MTR_2g029120 45.741 317 141 8 14 312 107 410 2.20e-79 251
MS.gene66688.t1 MTR_2g028380 47.368 285 121 4 13 296 114 370 3.61e-79 249
MS.gene66688.t1 MTR_2g029120 46.512 301 126 7 14 307 107 379 5.93e-79 246
MS.gene66688.t1 MTR_2g029125 46.622 296 126 6 14 305 155 422 3.31e-78 247
MS.gene66688.t1 MTR_2g029125 46.622 296 126 6 14 305 155 422 3.73e-78 247
MS.gene66688.t1 MTR_2g029125 46.622 296 126 6 14 305 155 422 4.68e-78 247
MS.gene66688.t1 MTR_2g029125 46.622 296 126 6 14 305 155 422 5.10e-78 247
MS.gene66688.t1 MTR_2g029125 46.622 296 126 6 14 305 155 422 5.11e-78 247
MS.gene66688.t1 MTR_2g029125 46.622 296 126 6 14 305 155 422 1.38e-77 248
MS.gene66688.t1 MTR_2g029125 46.622 296 126 6 14 305 155 422 2.36e-77 246
MS.gene66688.t1 MTR_7g044770 46.591 264 121 2 50 312 1 245 5.47e-77 246
MS.gene66688.t1 MTR_2g067200 43.667 300 144 4 15 312 169 445 2.14e-76 243
MS.gene66688.t1 MTR_2g028440 40.432 324 133 5 11 296 146 447 1.37e-69 228
MS.gene66688.t1 MTR_2g028400 44.755 286 129 4 14 298 140 397 1.02e-67 221
MS.gene66688.t1 MTR_8g105040 63.277 177 28 2 149 325 1 140 2.07e-63 199
MS.gene66688.t1 MTR_2g028340 44.726 237 111 2 11 246 178 395 8.04e-62 202
MS.gene66688.t1 MTR_2g066890 42.972 249 121 3 15 256 16 250 1.45e-61 197
MS.gene66688.t1 MTR_2g028360 46.818 220 97 2 13 231 10 210 3.06e-58 198
MS.gene66688.t1 MTR_2g028360 39.721 287 143 5 12 296 222 480 2.06e-56 194
MS.gene66688.t1 MTR_4g101750 30.820 305 190 6 12 313 167 453 8.40e-39 146
MS.gene66688.t1 MTR_4g101750 30.820 305 190 6 12 313 167 453 8.43e-39 146
MS.gene66688.t1 MTR_2g067190 43.931 173 81 2 15 187 129 285 1.36e-37 136
MS.gene66688.t1 MTR_2g028300 42.568 148 65 2 14 160 78 206 9.73e-33 121
MS.gene66688.t1 MTR_5g083100 38.750 160 52 2 158 312 265 383 2.20e-20 92.0
MS.gene66688.t1 MTR_2g021910 35.260 173 70 3 78 250 413 543 3.86e-20 92.0
MS.gene66688.t1 MTR_2g021910 34.848 132 67 1 77 208 47 159 2.60e-16 80.5
MS.gene66688.t1 MTR_8g105070 77.586 58 11 2 264 320 5 61 2.93e-19 82.8
MS.gene66688.t1 MTR_2g029210 44.118 68 38 0 259 326 42 109 1.69e-11 63.2
MS.gene66688.t1 MTR_2g066880 54.167 48 22 0 265 312 5 52 7.80e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66688.t1 AT1G45332 46.358 302 142 2 12 312 143 425 6.34e-90 285
MS.gene66688.t1 AT2G45030 46.358 302 142 2 12 312 143 425 1.16e-89 284
MS.gene66688.t1 AT1G45332 45.076 264 125 2 50 312 1 245 4.62e-75 242
MS.gene66688.t1 AT1G62750 30.492 305 191 6 12 313 171 457 4.86e-40 150

Find 48 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGTTCTTTCTTGTGTTGA+TGG 0.147617 4.3:+48099344 MS.gene66688:CDS
TGAGGCGTTAGAACTATTTA+TGG 0.231813 4.3:+48099880 MS.gene66688:CDS
AACTTATCTTAGAATCTATC+AGG 0.249865 4.3:+48100057 MS.gene66688:CDS
CTCTCATCACCAATGATAAT+TGG 0.285305 4.3:-48099854 None:intergenic
TGTTGATCTTGTCAAGTTAG+AGG 0.299617 4.3:+48099559 MS.gene66688:CDS
TTTGATGCTCATCTTGAATC+TGG 0.300024 4.3:+48100213 MS.gene66688:CDS
TATTTATGGAGGGTGTAATT+AGG 0.307222 4.3:+48099894 MS.gene66688:CDS
GAAGATTCAGATTCCTCGTT+TGG 0.327402 4.3:+48100126 MS.gene66688:CDS
CACATTGACGATTGTTATGT+TGG 0.346186 4.3:+48099533 MS.gene66688:CDS
GCTTATATTTATTGACGATC+TGG 0.368085 4.3:+48099424 MS.gene66688:CDS
CACAAAGGCTTGCATATCTT+CGG 0.381625 4.3:-48099690 None:intergenic
GCAAAAGCCTTTGCAACAAA+TGG 0.400513 4.3:-48100007 None:intergenic
ATCGGGAAGGAGCAGATCTA+TGG 0.411943 4.3:+48099447 MS.gene66688:CDS
TAAAACCGAATCCATTACTA+TGG 0.416829 4.3:+48099370 MS.gene66688:CDS
AAGATTCAGATTCCTCGTTT+GGG 0.418947 4.3:+48100127 MS.gene66688:CDS
AAATTTGTACCAATTATCAT+TGG 0.426791 4.3:+48099845 MS.gene66688:CDS
TTGATCCATAGTAATGGATT+CGG 0.433471 4.3:-48099375 None:intergenic
ACTTCTACATCATTATAATC+AGG 0.443013 4.3:-48099656 None:intergenic
TAACAATCGTCAATGTGTAT+TGG 0.446757 4.3:-48099527 None:intergenic
TGACAAGATTTCAACTTCCT+AGG 0.454857 4.3:+48099402 MS.gene66688:CDS
GGTGAGAAAGTTGAATTGTC+TGG 0.460058 4.3:+48099971 MS.gene66688:CDS
CATTTGTTGATCCATAGTAA+TGG 0.470087 4.3:-48099381 None:intergenic
AAAGTCTAACAAACATGTTA+AGG 0.481772 4.3:-48099595 None:intergenic
ATTTATTGACGATCTGGATC+GGG 0.488083 4.3:+48099430 MS.gene66688:CDS
TGATGCACCATTTGTTGCAA+AGG 0.499606 4.3:+48100000 MS.gene66688:CDS
GCTCTTGATCTAAATAAGAA+TGG 0.506175 4.3:+48099950 MS.gene66688:CDS
CTATCAGGGTGTTATTAAGA+AGG 0.508785 4.3:+48100072 MS.gene66688:CDS
GGAAGTTGATGAAGCTATGC+TGG 0.511565 4.3:+48100174 MS.gene66688:CDS
TATTTATTGACGATCTGGAT+CGG 0.519096 4.3:+48099429 MS.gene66688:CDS
ACTCATTGAAATTGTATCAG+AGG 0.540690 4.3:+48099730 MS.gene66688:CDS
TTACTAACATCGATTGGACT+TGG 0.543188 4.3:-48099923 None:intergenic
TTTATTACAAATGTTAACAC+TGG 0.545461 4.3:+48100100 MS.gene66688:CDS
GCATAATTACTAACATCGAT+TGG 0.547196 4.3:-48099929 None:intergenic
GATTATAATGATGTAGAAGT+AGG 0.547209 4.3:+48099659 MS.gene66688:CDS
GAGACATGACGATCTGATTG+AGG 0.560910 4.3:+48100153 MS.gene66688:CDS
ACAAAGGCTTGCATATCTTC+GGG 0.570024 4.3:-48099689 None:intergenic
GGCGTTAGAACTATTTATGG+AGG 0.572694 4.3:+48099883 MS.gene66688:CDS
GCATCATCGAGAACAGACTC+TGG 0.573694 4.3:-48099797 None:intergenic
CATTGGTGATGAGAGCTATG+AGG 0.582206 4.3:+48099862 MS.gene66688:CDS
TCGTCAATAAATATAAGCCT+AGG 0.585899 4.3:-48099419 None:intergenic
TTCTCGATGATGCAATTCGT+AGG 0.597250 4.3:+48099807 MS.gene66688:CDS
TATCAGGGTGTTATTAAGAA+GGG 0.606667 4.3:+48100073 MS.gene66688:CDS
TCTCGATGATGCAATTCGTA+GGG 0.618739 4.3:+48099808 MS.gene66688:CDS
GCGTTAGAACTATTTATGGA+GGG 0.620519 4.3:+48099884 MS.gene66688:CDS
TGTATATCTAACAGACCCAT+CGG 0.623464 4.3:-48100247 None:intergenic
ACTTATCTTAGAATCTATCA+GGG 0.629324 4.3:+48100058 MS.gene66688:CDS
ATTGACGATCTGGATCGGGA+AGG 0.672041 4.3:+48099434 MS.gene66688:CDS
TCATGTCTCTTACCCAAACG+AGG 0.712232 4.3:-48100139 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAATGTTAATATTAGAA+AGG + chr4.3:48098771-48098790 MS.gene66688:intron 15.0%
!! AAGAATGTTAATATTAGAAA+GGG + chr4.3:48098772-48098791 MS.gene66688:intron 15.0%
!! TAACTATTAATTCAATGATT+TGG + chr4.3:48098702-48098721 MS.gene66688:intron 15.0%
!! TTCTAATATTAACATTCTTT+CGG - chr4.3:48098771-48098790 None:intergenic 15.0%
!!! ACATGATATTTTCTAAAATA+CGG - chr4.3:48098478-48098497 None:intergenic 15.0%
!!! TTCTAAAAAAATATGAATTC+TGG + chr4.3:48098148-48098167 MS.gene66688:intron 15.0%
!! AAATTTGTACCAATTATCAT+TGG + chr4.3:48099845-48099864 MS.gene66688:CDS 20.0%
!! TCTAATATTAACATTCTTTC+GGG - chr4.3:48098770-48098789 None:intergenic 20.0%
!! TTTATTACAAATGTTAACAC+TGG + chr4.3:48100100-48100119 MS.gene66688:CDS 20.0%
!!! TAGACTTTTTGATAAGTTAA+AGG + chr4.3:48099610-48099629 MS.gene66688:CDS 20.0%
! AAAGTCTAACAAACATGTTA+AGG - chr4.3:48099598-48099617 None:intergenic 25.0%
! AACTTATCTTAGAATCTATC+AGG + chr4.3:48100057-48100076 MS.gene66688:CDS 25.0%
! ACTTATCTTAGAATCTATCA+GGG + chr4.3:48100058-48100077 MS.gene66688:CDS 25.0%
! ACTTCTACATCATTATAATC+AGG - chr4.3:48099659-48099678 None:intergenic 25.0%
! GATTATAATGATGTAGAAGT+AGG + chr4.3:48099659-48099678 MS.gene66688:CDS 25.0%
! TAAGCAACAAATGTCAAATA+GGG - chr4.3:48099237-48099256 None:intergenic 25.0%
! TACTACACTTAATGTTTGTT+AGG + chr4.3:48098850-48098869 MS.gene66688:intron 25.0%
!! ATGGTTTAAAACGATAGAAT+GGG + chr4.3:48098347-48098366 MS.gene66688:intron 25.0%
!!! AAACTTTCTAAAGCTTTTCA+TGG + chr4.3:48099761-48099780 MS.gene66688:CDS 25.0%
!!! AAATAGGGTTCTGTTTTTAT+AGG - chr4.3:48099222-48099241 None:intergenic 25.0%
!!! ATTCTATCGTTTTAAACCAT+GGG - chr4.3:48098347-48098366 None:intergenic 25.0%
!!! GATTTTTGAAGTAAAAGTAC+CGG - chr4.3:48098407-48098426 None:intergenic 25.0%
!!! TTGTTGCTTAGATACTTTTT+TGG + chr4.3:48099247-48099266 MS.gene66688:intron 25.0%
ACCAATACACTCAAATCTTT+GGG + chr4.3:48098728-48098747 MS.gene66688:intron 30.0%
ACCTAACAAACATTAAGATG+CGG - chr4.3:48098825-48098844 None:intergenic 30.0%
ACTCATTGAAATTGTATCAG+AGG + chr4.3:48099730-48099749 MS.gene66688:CDS 30.0%
CTAAGCAACAAATGTCAAAT+AGG - chr4.3:48099238-48099257 None:intergenic 30.0%
GCATAATTACTAACATCGAT+TGG - chr4.3:48099932-48099951 None:intergenic 30.0%
GCTCTTGATCTAAATAAGAA+TGG + chr4.3:48099950-48099969 MS.gene66688:CDS 30.0%
GCTTATATTTATTGACGATC+TGG + chr4.3:48099424-48099443 MS.gene66688:CDS 30.0%
TAAAACCGAATCCATTACTA+TGG + chr4.3:48099370-48099389 MS.gene66688:CDS 30.0%
TAACAATCGTCAATGTGTAT+TGG - chr4.3:48099530-48099549 None:intergenic 30.0%
TACCAATACACTCAAATCTT+TGG + chr4.3:48098727-48098746 MS.gene66688:intron 30.0%
TATTTATTGACGATCTGGAT+CGG + chr4.3:48099429-48099448 MS.gene66688:CDS 30.0%
TCGTCAATAAATATAAGCCT+AGG - chr4.3:48099422-48099441 None:intergenic 30.0%
! ATAGGAAGTGACATATGTTT+TGG - chr4.3:48099204-48099223 None:intergenic 30.0%
! CATGGTTTAAAACGATAGAA+TGG + chr4.3:48098346-48098365 MS.gene66688:intron 30.0%
! CATTTGTTGATCCATAGTAA+TGG - chr4.3:48099384-48099403 None:intergenic 30.0%
! TATTTATGGAGGGTGTAATT+AGG + chr4.3:48099894-48099913 MS.gene66688:CDS 30.0%
!! CATTCTATCGTTTTAAACCA+TGG - chr4.3:48098348-48098367 None:intergenic 30.0%
!! TATCAGGGTGTTATTAAGAA+GGG + chr4.3:48100073-48100092 MS.gene66688:CDS 30.0%
!! TGTTGGTAATTATGATGACA+TGG - chr4.3:48098288-48098307 None:intergenic 30.0%
!! TTCTATCGTTTTAAACCATG+GGG - chr4.3:48098346-48098365 None:intergenic 30.0%
!! TTGATCCATAGTAATGGATT+CGG - chr4.3:48099378-48099397 None:intergenic 30.0%
AAGATTCAGATTCCTCGTTT+GGG + chr4.3:48100127-48100146 MS.gene66688:CDS 35.0%
ACCCAAAGATTTGAGTGTAT+TGG - chr4.3:48098732-48098751 None:intergenic 35.0%
ATTCTTTCGGGCAAATTGTA+TGG - chr4.3:48098758-48098777 None:intergenic 35.0%
ATTTATTGACGATCTGGATC+GGG + chr4.3:48099430-48099449 MS.gene66688:CDS 35.0%
CACATTGACGATTGTTATGT+TGG + chr4.3:48099533-48099552 MS.gene66688:CDS 35.0%
CATGTTAAGGCGAAAAAAGT+AGG - chr4.3:48099585-48099604 None:intergenic 35.0%
CTCTCATCACCAATGATAAT+TGG - chr4.3:48099857-48099876 None:intergenic 35.0%
CTTGTTCTTTCTTGTGTTGA+TGG + chr4.3:48099344-48099363 MS.gene66688:CDS 35.0%
GCGTTAGAACTATTTATGGA+GGG + chr4.3:48099884-48099903 MS.gene66688:CDS 35.0%
TGACAAGATTTCAACTTCCT+AGG + chr4.3:48099402-48099421 MS.gene66688:CDS 35.0%
TGTATATCTAACAGACCCAT+CGG - chr4.3:48100250-48100269 None:intergenic 35.0%
TGTTGATCTTGTCAAGTTAG+AGG + chr4.3:48099559-48099578 MS.gene66688:CDS 35.0%
TGTTTCAGAGCGAAAAAAGT+AGG - chr4.3:48099636-48099655 None:intergenic 35.0%
TTTGATGCTCATCTTGAATC+TGG + chr4.3:48100213-48100232 MS.gene66688:CDS 35.0%
! TGAGGCGTTAGAACTATTTA+TGG + chr4.3:48099880-48099899 MS.gene66688:CDS 35.0%
! TTACTAACATCGATTGGACT+TGG - chr4.3:48099926-48099945 None:intergenic 35.0%
! TTGCAAAGGCTTTTGCAAAT+TGG + chr4.3:48100014-48100033 MS.gene66688:CDS 35.0%
!! CTATCAGGGTGTTATTAAGA+AGG + chr4.3:48100072-48100091 MS.gene66688:CDS 35.0%
!!! CTTTCTAAAGCTTTTCATGG+TGG + chr4.3:48099764-48099783 MS.gene66688:CDS 35.0%
AAACATTAAGATGCGGCAAG+GGG - chr4.3:48098818-48098837 None:intergenic 40.0%
ACAAACATTAAGATGCGGCA+AGG - chr4.3:48098820-48098839 None:intergenic 40.0%
ACAAAGGCTTGCATATCTTC+GGG - chr4.3:48099692-48099711 None:intergenic 40.0%
AGATGGATAAATAGAGGAGG+CGG - chr4.3:48098982-48099001 None:intergenic 40.0%
CAAACATTAAGATGCGGCAA+GGG - chr4.3:48098819-48098838 None:intergenic 40.0%
CACAAAGGCTTGCATATCTT+CGG - chr4.3:48099693-48099712 None:intergenic 40.0%
CCATGAATCGTTTCTTCTCA+AGG + chr4.3:48098913-48098932 MS.gene66688:intron 40.0%
CCTTGAGAAGAAACGATTCA+TGG - chr4.3:48098916-48098935 None:intergenic 40.0%
CGATAGAATGGGAACTTCTA+CGG + chr4.3:48098358-48098377 MS.gene66688:intron 40.0%
GAAGATTCAGATTCCTCGTT+TGG + chr4.3:48100126-48100145 MS.gene66688:CDS 40.0%
GATACGACTACGTTACTAAG+CGG + chr4.3:48099084-48099103 MS.gene66688:intron 40.0%
GCAAAAGCCTTTGCAACAAA+TGG - chr4.3:48100010-48100029 None:intergenic 40.0%
GCCGCATCTTAATGTTTGTT+AGG + chr4.3:48098821-48098840 MS.gene66688:intron 40.0%
GGAAGATGGATAAATAGAGG+AGG - chr4.3:48098985-48099004 None:intergenic 40.0%
GGAGGAAGATGGATAAATAG+AGG - chr4.3:48098988-48099007 None:intergenic 40.0%
GGCGTTAGAACTATTTATGG+AGG + chr4.3:48099883-48099902 MS.gene66688:CDS 40.0%
GGTGAGAAAGTTGAATTGTC+TGG + chr4.3:48099971-48099990 MS.gene66688:CDS 40.0%
TCTCGATGATGCAATTCGTA+GGG + chr4.3:48099808-48099827 MS.gene66688:CDS 40.0%
TGAGAAGAAACGATTCATGG+CGG - chr4.3:48098913-48098932 None:intergenic 40.0%
TTCTCGATGATGCAATTCGT+AGG + chr4.3:48099807-48099826 MS.gene66688:CDS 40.0%
! ACCGGTACACCTTAATTTTG+CGG - chr4.3:48098389-48098408 None:intergenic 40.0%
! CTGGAGATACTTTTACCGAT+GGG + chr4.3:48100232-48100251 MS.gene66688:CDS 40.0%
! CTTTTGCAAATTGGCATGAG+AGG + chr4.3:48100023-48100042 MS.gene66688:CDS 40.0%
! GCGTCTTTTCTTCAACACAA+AGG - chr4.3:48099708-48099727 None:intergenic 40.0%
! TCTGGAGATACTTTTACCGA+TGG + chr4.3:48100231-48100250 MS.gene66688:CDS 40.0%
! TGATGCACCATTTGTTGCAA+AGG + chr4.3:48100000-48100019 MS.gene66688:CDS 40.0%
AAACGTGGGTCGTCATAGTT+CGG - chr4.3:48099149-48099168 None:intergenic 45.0%
ACGGTACACCCGCAAAATTA+AGG + chr4.3:48098377-48098396 MS.gene66688:intron 45.0%
CCCGCAAAATTAAGGTGTAC+CGG + chr4.3:48098385-48098404 MS.gene66688:intron 45.0%
CGTCCTCGTATACAAAACGT+GGG - chr4.3:48099163-48099182 None:intergenic 45.0%
GAGACATGACGATCTGATTG+AGG + chr4.3:48100153-48100172 MS.gene66688:CDS 45.0%
TCACTTCTCCAACAAGTCAG+TGG + chr4.3:48099034-48099053 MS.gene66688:intron 45.0%
TCATGTCTCTTACCCAAACG+AGG - chr4.3:48100142-48100161 None:intergenic 45.0%
TCGTCCTCGTATACAAAACG+TGG - chr4.3:48099164-48099183 None:intergenic 45.0%
TGTCTTGTCAGATTACCCCA+TGG + chr4.3:48098328-48098347 MS.gene66688:intron 45.0%
! CCGGTACACCTTAATTTTGC+GGG - chr4.3:48098388-48098407 None:intergenic 45.0%
!! CATTGGTGATGAGAGCTATG+AGG + chr4.3:48099862-48099881 MS.gene66688:CDS 45.0%
!! CTGACTTGTTGGAGAAGTGA+CGG - chr4.3:48099034-48099053 None:intergenic 45.0%
!! GGAAGTTGATGAAGCTATGC+TGG + chr4.3:48100174-48100193 MS.gene66688:CDS 45.0%
!! GATTTAATTATTATAATTAT+AGG + chr4.3:48098643-48098662 MS.gene66688:intron 5.0%
AAACGATTCATGGCGGCAAG+GGG - chr4.3:48098906-48098925 None:intergenic 50.0%
ACGTTACTAAGCGGACAGAC+AGG + chr4.3:48099093-48099112 MS.gene66688:intron 50.0%
AGAAACGATTCATGGCGGCA+AGG - chr4.3:48098908-48098927 None:intergenic 50.0%
ATAAATAGAGGAGGCGGAGG+AGG - chr4.3:48098976-48098995 None:intergenic 50.0%
ATCGGGAAGGAGCAGATCTA+TGG + chr4.3:48099447-48099466 MS.gene66688:CDS 50.0%
ATTGACGATCTGGATCGGGA+AGG + chr4.3:48099434-48099453 MS.gene66688:CDS 50.0%
CGTATCCAGAAATCCAGACG+TGG - chr4.3:48099070-48099089 None:intergenic 50.0%
CGTTACTAAGCGGACAGACA+GGG + chr4.3:48099094-48099113 MS.gene66688:intron 50.0%
GAAACGATTCATGGCGGCAA+GGG - chr4.3:48098907-48098926 None:intergenic 50.0%
GCATCATCGAGAACAGACTC+TGG - chr4.3:48099800-48099819 None:intergenic 50.0%
TAAGATGCGGCAAGGGGTTT+GGG - chr4.3:48098812-48098831 None:intergenic 50.0%
TGGATAAATAGAGGAGGCGG+AGG - chr4.3:48098979-48098998 None:intergenic 50.0%
TGGGTCGTCATAGTTCGGAT+AGG - chr4.3:48099144-48099163 None:intergenic 50.0%
TTAAGATGCGGCAAGGGGTT+TGG - chr4.3:48098813-48098832 None:intergenic 50.0%
! ACTTGTTGGAGAAGTGACGG+AGG - chr4.3:48099031-48099050 None:intergenic 50.0%
! CGACCCACGTTTTGTATACG+AGG + chr4.3:48099157-48099176 MS.gene66688:intron 50.0%
AGCGTACGGTGGAGTAGAGA+AGG - chr4.3:48098953-48098972 None:intergenic 55.0%
TCGCTGATGTGTCTCGCTGT+TGG - chr4.3:48098305-48098324 None:intergenic 55.0%
! AGAAGTGACGGAGGTGGAGA+GGG - chr4.3:48099022-48099041 None:intergenic 55.0%
! TGTTGGAGAAGTGACGGAGG+TGG - chr4.3:48099028-48099047 None:intergenic 55.0%
!! CAGCTGCTCCACTGACTTGT+TGG - chr4.3:48099045-48099064 None:intergenic 55.0%
ACGGTGGAGTAGAGAAGGCG+TGG - chr4.3:48098948-48098967 None:intergenic 60.0%
CATGGCGGCAAGGGGTTTGA+TGG - chr4.3:48098898-48098917 None:intergenic 60.0%
CTGCGCCACGTCTGGATTTC+TGG + chr4.3:48099062-48099081 MS.gene66688:intron 60.0%
CTGGGGAGAACGGAGGAAGA+TGG - chr4.3:48098999-48099018 None:intergenic 60.0%
! GAGAAGTGACGGAGGTGGAG+AGG - chr4.3:48099023-48099042 None:intergenic 60.0%
! GGAGTAGAGAAGGCGTGGCA+AGG - chr4.3:48098943-48098962 None:intergenic 60.0%
! GTAGAGAAGGCGTGGCAAGG+AGG - chr4.3:48098940-48098959 None:intergenic 60.0%
GTGGAGAGGGCTGGGGAGAA+CGG - chr4.3:48099009-48099028 None:intergenic 65.0%
TGACGGAGGTGGAGAGGGCT+GGG - chr4.3:48099017-48099036 None:intergenic 65.0%
GACGGAGGTGGAGAGGGCTG+GGG - chr4.3:48099016-48099035 None:intergenic 70.0%
GAGAGGGCTGGGGAGAACGG+AGG - chr4.3:48099006-48099025 None:intergenic 70.0%
TGGAGCAGCTGCGCCACGTC+TGG + chr4.3:48099054-48099073 MS.gene66688:intron 70.0%
! GGAGGCGGAGGAGGAGCGTA+CGG - chr4.3:48098967-48098986 None:intergenic 70.0%
! GTGACGGAGGTGGAGAGGGC+TGG - chr4.3:48099018-48099037 None:intergenic 70.0%
! GGCGGAGGAGGAGCGTACGG+TGG - chr4.3:48098964-48098983 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 48097949 48100273 48097949 ID=MS.gene66688
chr4.3 mRNA 48097949 48100273 48097949 ID=MS.gene66688.t1;Parent=MS.gene66688
chr4.3 exon 48097949 48097952 48097949 ID=MS.gene66688.t1.exon1;Parent=MS.gene66688.t1
chr4.3 CDS 48097949 48097952 48097949 ID=cds.MS.gene66688.t1;Parent=MS.gene66688.t1
chr4.3 exon 48099258 48100273 48099258 ID=MS.gene66688.t1.exon2;Parent=MS.gene66688.t1
chr4.3 CDS 48099258 48100273 48099258 ID=cds.MS.gene66688.t1;Parent=MS.gene66688.t1
Gene Sequence

>MS.gene66688

ATGAGTAAAAAACTAAATATTATAATTTTTTGTAATTTTTTTTAAATATTCTAGAAAATAATTAATTTTTTCGAAAAAACTTTTTATTTTTTCTAAAAAAGAAAATTCTTGAAAATTAGATAAGTTTATTTTTATTTTAAAAAAAATAAATATTCTAGAAAATAAAAAATATTTTTTTTGAAATAAAACTTTTTATTTTTTCTAAAAAAATATGAATTCTGGAAAAAAAACTTTTCTATTTTTTCTAAAAATATTTATTTTTTATTTTTTAAATAATAAAAAAACAGTTGAAAATAAAAATAAATGAAAAATAATTTTAAAAGCGTAGTAAGCATGCCATGTCATCATAATTACCAACAGCGAGACACATCAGCGATTTTGTCTTGTCAGATTACCCCATGGTTTAAAACGATAGAATGGGAACTTCTACGGTACACCCGCAAAATTAAGGTGTACCGGTACTTTTACTTCAAAAATCTATAAATTAACTATCATTTTTATGAAATAAAGTGCTGTTTGTTGATATCCGTATTTTAGAAAATATCATGTCATAAGAAAAACATCATTTCATTGTTTATAAAAATTATACTAATTTTTTAACATTTTATTGTAAAAAATAATCAAAATTCTCTATTATGAGTACTAAAAATTCATAAAAATTAAATTTTATTTCGTTGACGTTTTTGATAAATAAGATTTAATTATTATAATTATAGGTGATAGAAAAAAATTTTCTGTAAAAAATAACACATTTAACTATTAATTCAATGATTTGGTGTACCAATACACTCAAATCTTTGGGTGTACCATACAATTTGCCCGAAAGAATGTTAATATTAGAAAGGGTAAACGCATATTAACCCAAACCCCTTGCCGCATCTTAATGTTTGTTAGGTTTGCTTACTACACTTAATGTTTGTTAGGTTTGCTTACTACACTGTAGCTTCCATCAAACCCCTTGCCGCCATGAATCGTTTCTTCTCAAGGTCCTCCTTGCCACGCCTTCTCTACTCCACCGTACGCTCCTCCTCCGCCTCCTCTATTTATCCATCTTCCTCCGTTCTCCCCAGCCCTCTCCACCTCCGTCACTTCTCCAACAAGTCAGTGGAGCAGCTGCGCCACGTCTGGATTTCTGGATACGACTACGTTACTAAGCGGACAGACAGGGAAATAGTATTATTAGAACGTCATTCCTATCCGAACTATGACGACCCACGTTTTGTATACGAGGACGAGATAGACAACATACATACCAAAACATATGTCACTTCCTATAAAAACAGAACCCTATTTGACATTTGTTGCTTAGATACTTTTTTGGAGAAGATATGTTCTGATCATGTTGATTTCAATGTTGATGTTCAACTAGCTTCGCGTATTTTTGACAGTGCAATTCTTGTTCTTTCTTGTGTTGATGGTGTTAAAACCGAATCCATTACTATGGATCAACAAATGACAAGATTTCAACTTCCTAGGCTTATATTTATTGACGATCTGGATCGGGAAGGAGCAGATCTATGGAACATTGTAGATCAAGTAAAATCTAAGCTTAATCATCGTTGTGCTGCAATTCAAGTTCCAATACACATTGACGATTGTTATGTTGGATTTGTTGATCTTGTCAAGTTAGAGGCCTACTTTTTTCGCCTTAACATGTTTGTTAGACTTTTTGATAAGTTAAAGGCCTACTTTTTTCGCTCTGAAACACCTGATTATAATGATGTAGAAGTAGGAGAAATACCCGAAGATATGCAAGCCTTTGTGTTGAAGAAAAGACGCAAACTCATTGAAATTGTATCAGAGGTTGATGATAAACTTTCTAAAGCTTTTCATGGTGGCAGCGAAATTCCAGAGTCTGTTCTCGATGATGCAATTCGTAGGGCAACTATATCAATGAAATTTGTACCAATTATCATTGGTGATGAGAGCTATGAGGCGTTAGAACTATTTATGGAGGGTGTAATTAGGTATTTGCCAAGTCCAATCGATGTTAGTAATTATGCTCTTGATCTAAATAAGAATGGTGAGAAAGTTGAATTGTCTGGAAGTATTGATGCACCATTTGTTGCAAAGGCTTTTGCAAATTGGCATGAGAGGTATTTAAAATTAACTTATCTTAGAATCTATCAGGGTGTTATTAAGAAGGGTGATTTTATTACAAATGTTAACACTGGTAAGAAGATTCAGATTCCTCGTTTGGGTAAGAGACATGACGATCTGATTGAGGAAGTTGATGAAGCTATGCTGGAGAAATTGTTTTTGTTTTTGATGCTCATCTTGAATCTGGAGATACTTTTACCGATGGGTCTGTTAGATATACAATGA
Protein sequence

>MS.gene66688.t1

MNTFLEKICSDHVDFNVDVQLASRIFDSAILVLSCVDGVKTESITMDQQMTRFQLPRLIFIDDLDREGADLWNIVDQVKSKLNHRCAAIQVPIHIDDCYVGFVDLVKLEAYFFRLNMFVRLFDKLKAYFFRSETPDYNDVEVGEIPEDMQAFVLKKRRKLIEIVSEVDDKLSKAFHGGSEIPESVLDDAIRRATISMKFVPIIIGDESYEALELFMEGVIRYLPSPIDVSNYALDLNKNGEKVELSGSIDAPFVAKAFANWHERYLKLTYLRIYQGVIKKGDFITNVNTGKKIQIPRLGKRHDDLIEEVDEAMLEKLFLFLMLILNLEILLPMGLLDIQ