Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66843.t1 | XP_013465955.1 | 98.9 | 183 | 2 | 0 | 1 | 183 | 1 | 183 | 8.00E-85 | 323.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66843.t1 | P08927 | 87.8 | 181 | 22 | 0 | 3 | 183 | 6 | 186 | 5.8e-76 | 285.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66843.t1 | A0A072VEG8 | 98.9 | 183 | 2 | 0 | 1 | 183 | 1 | 183 | 5.7e-85 | 323.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049297 | MS.gene66843 | 0.802383 | 5.76E-49 | -1.69E-46 |
| MS.gene051662 | MS.gene66843 | 0.802629 | 5.12E-49 | -1.69E-46 |
| MS.gene051663 | MS.gene66843 | 0.829795 | 4.21E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66843.t1 | MTR_0991s0010 | 97.268 | 183 | 5 | 0 | 1 | 183 | 1 | 183 | 6.00e-125 | 350 |
| MS.gene66843.t1 | MTR_1g017380 | 98.907 | 183 | 2 | 0 | 1 | 183 | 1 | 183 | 2.22e-121 | 355 |
| MS.gene66843.t1 | MTR_1g017390 | 97.268 | 183 | 5 | 0 | 1 | 183 | 1 | 183 | 4.86e-119 | 349 |
| MS.gene66843.t1 | MTR_4g057970 | 46.561 | 189 | 81 | 4 | 6 | 182 | 1 | 181 | 4.31e-45 | 156 |
| MS.gene66843.t1 | MTR_4g084130 | 44.970 | 169 | 90 | 2 | 10 | 178 | 4 | 169 | 9.52e-38 | 136 |
| MS.gene66843.t1 | MTR_1g090140 | 45.509 | 167 | 82 | 2 | 12 | 178 | 6 | 163 | 7.83e-36 | 131 |
| MS.gene66843.t1 | MTR_1g090130 | 44.311 | 167 | 84 | 3 | 12 | 178 | 6 | 163 | 5.57e-34 | 126 |
| MS.gene66843.t1 | MTR_8g017080 | 43.165 | 139 | 73 | 2 | 45 | 178 | 27 | 164 | 3.10e-28 | 110 |
| MS.gene66843.t1 | MTR_7g067470 | 36.364 | 110 | 64 | 2 | 65 | 172 | 26 | 131 | 4.46e-12 | 63.9 |
| MS.gene66843.t1 | MTR_7g113470 | 36.364 | 110 | 64 | 2 | 65 | 172 | 26 | 131 | 5.05e-12 | 63.9 |
| MS.gene66843.t1 | MTR_4g084130 | 54.545 | 55 | 25 | 0 | 124 | 178 | 3 | 57 | 9.06e-12 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66843.t1 | AT5G56500 | 90.541 | 148 | 13 | 1 | 36 | 183 | 42 | 188 | 2.54e-84 | 260 |
| MS.gene66843.t1 | AT5G56500 | 90.541 | 148 | 13 | 1 | 36 | 183 | 42 | 188 | 2.54e-84 | 260 |
| MS.gene66843.t1 | AT3G13470 | 89.865 | 148 | 14 | 1 | 36 | 183 | 42 | 188 | 4.29e-84 | 259 |
| MS.gene66843.t1 | AT1G55490 | 88.514 | 148 | 16 | 1 | 36 | 183 | 46 | 192 | 3.16e-82 | 254 |
| MS.gene66843.t1 | AT1G55490 | 88.514 | 148 | 16 | 1 | 36 | 183 | 46 | 192 | 3.16e-82 | 254 |
| MS.gene66843.t1 | AT1G55490 | 88.514 | 148 | 16 | 1 | 36 | 183 | 46 | 192 | 3.16e-82 | 254 |
| MS.gene66843.t1 | AT1G55490 | 88.514 | 148 | 16 | 1 | 36 | 183 | 46 | 192 | 3.16e-82 | 254 |
| MS.gene66843.t1 | AT1G55490 | 88.514 | 148 | 16 | 1 | 36 | 183 | 46 | 192 | 3.16e-82 | 254 |
| MS.gene66843.t1 | AT1G26230 | 62.428 | 173 | 63 | 1 | 13 | 183 | 4 | 176 | 7.27e-69 | 217 |
| MS.gene66843.t1 | AT1G26230 | 62.428 | 173 | 63 | 1 | 13 | 183 | 4 | 176 | 3.08e-68 | 217 |
| MS.gene66843.t1 | AT1G26230 | 62.428 | 173 | 63 | 1 | 13 | 183 | 4 | 176 | 1.03e-67 | 217 |
| MS.gene66843.t1 | AT1G26230 | 70.588 | 119 | 35 | 0 | 65 | 183 | 6 | 124 | 2.23e-54 | 181 |
| MS.gene66843.t1 | AT1G26230 | 72.174 | 115 | 32 | 0 | 69 | 183 | 2 | 116 | 1.01e-53 | 179 |
| MS.gene66843.t1 | AT2G28000 | 53.846 | 143 | 63 | 2 | 36 | 178 | 37 | 176 | 4.78e-43 | 151 |
| MS.gene66843.t1 | AT3G23990 | 44.379 | 169 | 81 | 3 | 10 | 178 | 7 | 162 | 1.11e-36 | 134 |
| MS.gene66843.t1 | AT5G18820 | 45.732 | 164 | 83 | 3 | 21 | 181 | 6 | 166 | 5.00e-35 | 127 |
| MS.gene66843.t1 | AT2G33210 | 43.293 | 164 | 86 | 4 | 17 | 178 | 5 | 163 | 7.47e-35 | 129 |
| MS.gene66843.t1 | AT2G33210 | 43.293 | 164 | 86 | 4 | 17 | 178 | 5 | 163 | 7.76e-35 | 129 |
| MS.gene66843.t1 | AT5G18820 | 45.732 | 164 | 83 | 3 | 21 | 181 | 6 | 166 | 2.58e-34 | 127 |
| MS.gene66843.t1 | AT3G13860 | 45.098 | 153 | 76 | 3 | 26 | 178 | 18 | 162 | 5.19e-31 | 118 |
| MS.gene66843.t1 | AT5G20890 | 35.922 | 103 | 60 | 2 | 65 | 165 | 26 | 124 | 8.56e-11 | 60.5 |
Find 0 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGAGTGAAATTTTATTTTAT+AGG | - | 10834:4401-4420 | MS.gene66843:CDS | 15.0% |
| !!! | TTTACTTGAATTATTGTTTT+AGG | - | 10834:3942-3961 | MS.gene66843:intron | 15.0% |
| !!! | TTTACTTGAATTATTGTTTT+TGG | - | 10834:3942-3961 | MS.gene66843:intron | 15.0% |
| !!! | TTTTAGTGAATTAATAATGT+GGG | - | 10834:4113-4132 | MS.gene66843:intron | 15.0% |
| !!! | TTTTAGTGAATTATTTATGT+GGG | - | 10834:3841-3860 | MS.gene66843:intron | 15.0% |
| !!! | TTTTTAGTGAATTAATAATG+TGG | - | 10834:4112-4131 | MS.gene66843:intron | 15.0% |
| !!! | TTTTTAGTGAATTATTTATG+TGG | - | 10834:3840-3859 | MS.gene66843:intron | 15.0% |
| !! | TTTAGTGAATTAATAATGTG+GGG | - | 10834:4114-4133 | MS.gene66843:intron | 20.0% |
| !! | TTTAGTGAATTATTTATGTG+GGG | - | 10834:3842-3861 | MS.gene66843:intron | 20.0% |
| !!! | ATTTATTTTGTCTGAGTTTA+GGG | - | 10834:3911-3930 | MS.gene66843:intron | 20.0% |
| !!! | TAAAATTTCACTCTAAAATG+AGG | + | 10834:4395-4414 | MS.gene66843:intergenic | 20.0% |
| !!! | TATTTATTTTGTCTGAGTTT+AGG | - | 10834:3910-3929 | MS.gene66843:intron | 20.0% |
| !!! | TTCACTTGAATTAATGTTTT+TGG | - | 10834:3764-3783 | MS.gene66843:intron | 20.0% |
| !!! | TTCACTTGAATTATTGTTTT+TGG | - | 10834:3882-3901 | MS.gene66843:intron | 20.0% |
| ! | TAAGTAACTAATAATGTGCT+TGG | - | 10834:4338-4357 | MS.gene66843:CDS | 25.0% |
| ! | TGTTTACATTTGCGTTAAAA+AGG | - | 10834:4154-4173 | MS.gene66843:CDS | 25.0% |
| !! | TTTTGGTGAATTAGTTATGT+GGG | - | 10834:3959-3978 | MS.gene66843:intron | 25.0% |
| !!! | GTTTAATTTGTCTGAGTTTA+GGG | - | 10834:3793-3812 | MS.gene66843:intron | 25.0% |
| !!! | TGTTTAATTTGTCTGAGTTT+AGG | - | 10834:3792-3811 | MS.gene66843:intron | 25.0% |
| !!! | TTTGTGTTTTTGTTACAGAA+TGG | - | 10834:3520-3539 | MS.gene66843:CDS | 25.0% |
| !!! | TTTTAGGTGAATTAGTTATG+TGG | - | 10834:4035-4054 | MS.gene66843:intron | 25.0% |
| !!! | TTTTTGGTGAATTAGTTATG+TGG | - | 10834:3958-3977 | MS.gene66843:intron | 25.0% |
| AACTAATAATGTGCTTGGTT+TGG | - | 10834:4343-4362 | MS.gene66843:CDS | 30.0% | |
| ACACCATCATTAACAATCTT+AGG | + | 10834:3628-3647 | MS.gene66843:intergenic | 30.0% | |
| ACATCACAGAATTCAAAAAC+AGG | + | 10834:3688-3707 | MS.gene66843:intergenic | 30.0% | |
| GAGTTGTATTTCAACAAAGA+TGG | - | 10834:3406-3425 | MS.gene66843:intron | 30.0% | |
| TCTCCTAAGATTGTTAATGA+TGG | - | 10834:3622-3641 | MS.gene66843:intron | 30.0% | |
| TTACATTTGCGTTAAAAAGG+TGG | - | 10834:4157-4176 | MS.gene66843:CDS | 30.0% | |
| TTTGGTGAATTAGTTATGTG+GGG | - | 10834:3960-3979 | MS.gene66843:intron | 30.0% | |
| TTTGTTGAAATACAACTCCT+TGG | + | 10834:3404-3423 | MS.gene66843:intergenic | 30.0% | |
| ! | ATTATTTATGTGGGGTGTTT+AGG | - | 10834:3850-3869 | MS.gene66843:intron | 30.0% |
| ! | TTATTTATGTGGGGTGTTTA+GGG | - | 10834:3851-3870 | MS.gene66843:intron | 30.0% |
| !! | TGAGAATATTGGTGCTAAAT+TGG | - | 10834:4196-4215 | MS.gene66843:CDS | 30.0% |
| !! | TTGCTTGTTGTTACATGATT+GGG | - | 10834:4368-4387 | MS.gene66843:CDS | 30.0% |
| !! | TTTGCTTGTTGTTACATGAT+TGG | - | 10834:4367-4386 | MS.gene66843:CDS | 30.0% |
| !!! | TTCGTTTTTTGAGCATGATT+GGG | + | 10834:3464-3483 | MS.gene66843:intergenic | 30.0% |
| !!! | TTTCGTTTTTTGAGCATGAT+TGG | + | 10834:3465-3484 | MS.gene66843:intergenic | 30.0% |
| AGAACAACATTCCTTCCTTT+AGG | + | 10834:3586-3605 | MS.gene66843:intergenic | 35.0% | |
| AGAAGATGGGAATGAAGAAA+TGG | + | 10834:3311-3330 | MS.gene66843:intergenic | 35.0% | |
| TTAGCACCAATATTCTCAAC+TGG | + | 10834:4194-4213 | MS.gene66843:intergenic | 35.0% | |
| ! | ATTTTATAGGTTGTTGCAGC+TGG | - | 10834:4414-4433 | MS.gene66843:CDS | 35.0% |
| !! | AACTACTACCTCTGTTGTTT+TGG | - | 10834:4262-4281 | MS.gene66843:intron | 35.0% |
| !! | GTTTTGATGTTGGGTGTTTA+GGG | - | 10834:4065-4084 | MS.gene66843:intron | 35.0% |
| !! | TCTTATTGCAGAAGGTGTTA+AGG | - | 10834:4292-4311 | MS.gene66843:intron | 35.0% |
| !! | TGCTTGTTGTTACATGATTG+GGG | - | 10834:4369-4388 | MS.gene66843:CDS | 35.0% |
| !!! | GGAGTGTTTGTTTTGATGTT+GGG | - | 10834:4056-4075 | MS.gene66843:intron | 35.0% |
| !!! | GGTAGACTTGTTATGAGTTT+TGG | - | 10834:4313-4332 | MS.gene66843:intron | 35.0% |
| !!! | TGGAGTGTTTGTTTTGATGT+TGG | - | 10834:4055-4074 | MS.gene66843:intron | 35.0% |
| !!! | TGTTTTGATGTTGGGTGTTT+AGG | - | 10834:4064-4083 | MS.gene66843:intron | 35.0% |
| AAATGGAAGCGGATGAAGAA+AGG | + | 10834:3294-3313 | None:intergenic | 40.0% | |
| GAGGATCCAGTTGAGAATAT+TGG | - | 10834:4185-4204 | MS.gene66843:CDS | 40.0% | |
| GTTGTTCTTGAGAGCAAGTA+TGG | - | 10834:3598-3617 | MS.gene66843:intron | 40.0% | |
| TAAACAAACACACACCTCTC+TGG | + | 10834:3659-3678 | MS.gene66843:intergenic | 40.0% | |
| TGCCAAATCATTTGTCTTGG+CGG | + | 10834:4234-4253 | MS.gene66843:intergenic | 40.0% | |
| TGGGAATGAAGAAATGGAAG+CGG | + | 10834:3305-3324 | MS.gene66843:intergenic | 40.0% | |
| TTACTCTTGGTCCTAAAGGA+AGG | - | 10834:3572-3591 | MS.gene66843:CDS | 40.0% | |
| TTGCGTTAAAAAGGTGGAGT+TGG | - | 10834:4163-4182 | MS.gene66843:CDS | 40.0% | |
| ! | TGGTGCTAAATTGGTTAGAC+AGG | - | 10834:4205-4224 | MS.gene66843:CDS | 40.0% |
| ! | TTTGTCTGAGTTTAGGGTCA+AGG | - | 10834:3799-3818 | MS.gene66843:intron | 40.0% |
| ! | TTTTTCTGCGGGAAGAAGAT+GGG | + | 10834:3324-3343 | MS.gene66843:intergenic | 40.0% |
| !! | GATTTGGTTGGTGTTACTCT+TGG | - | 10834:3559-3578 | MS.gene66843:CDS | 40.0% |
| AGCCGCCAAGACAAATGATT+TGG | - | 10834:4229-4248 | MS.gene66843:intron | 45.0% | |
| CACCTGGAGTTTCTTGATAG+CGG | + | 10834:3434-3453 | MS.gene66843:intergenic | 45.0% | |
| CGTTAAAAAGGTGGAGTTGG+AGG | - | 10834:4166-4185 | MS.gene66843:CDS | 45.0% | |
| GAAATACAACTCCTTGGCCA+TGG | + | 10834:3398-3417 | MS.gene66843:intergenic | 45.0% | |
| GAATGGTGTGAACAAGCTTG+CGG | - | 10834:3537-3556 | MS.gene66843:CDS | 45.0% | |
| GCCTGCCAAATCATTTGTCT+TGG | + | 10834:4237-4256 | MS.gene66843:intergenic | 45.0% | |
| GCTATCAAGAAACTCCAGGT+GGG | - | 10834:3433-3452 | MS.gene66843:intron | 45.0% | |
| GGATGAAGAAAGGTTGCATG+AGG | + | 10834:3284-3303 | MS.gene66843:intergenic | 45.0% | |
| TGTGAACAAGCTTGCGGATT+TGG | - | 10834:3543-3562 | MS.gene66843:CDS | 45.0% | |
| TTCCGCTATCAAGAAACTCC+AGG | - | 10834:3429-3448 | MS.gene66843:intron | 45.0% | |
| TTTATGGCTCCGATTCTTCC+TGG | + | 10834:3368-3387 | MS.gene66843:intergenic | 45.0% | |
| ! | AACAAGCTTGCGGATTTGGT+TGG | - | 10834:3547-3566 | MS.gene66843:CDS | 45.0% |
| ! | ACAAATGATTTGGCAGGCGA+TGG | - | 10834:4239-4258 | MS.gene66843:intron | 45.0% |
| ! | GCCAAGACAAATGATTTGGC+AGG | - | 10834:4233-4252 | MS.gene66843:intron | 45.0% |
| ! | GTTTTTCTGCGGGAAGAAGA+TGG | + | 10834:3325-3344 | MS.gene66843:intergenic | 45.0% |
| !! | GGTGTTACTCTTGGTCCTAA+AGG | - | 10834:3568-3587 | MS.gene66843:CDS | 45.0% |
| !!! | ACCTCTGTTGTTTTGGCTCA+GGG | - | 10834:4269-4288 | MS.gene66843:intron | 45.0% |
| !!! | TACCTCTGTTGTTTTGGCTC+AGG | - | 10834:4268-4287 | MS.gene66843:intron | 45.0% |
| ACCCTGAGCCAAAACAACAG+AGG | + | 10834:4273-4292 | MS.gene66843:intergenic | 50.0% | |
| ATACCACGCGTGATAAGCAC+AGG | + | 10834:4447-4466 | MS.gene66843:intergenic | 50.0% | |
| ATGATTGGGAAGAACCCACC+TGG | + | 10834:3450-3469 | MS.gene66843:intergenic | 50.0% | |
| CGCTATCAAGAAACTCCAGG+TGG | - | 10834:3432-3451 | MS.gene66843:intron | 50.0% | |
| GAGCCATAAAGTCTCAGCCA+TGG | - | 10834:3378-3397 | MS.gene66843:intron | 50.0% | |
| TAAAGTCTCAGCCATGGCCA+AGG | - | 10834:3384-3403 | MS.gene66843:intron | 50.0% | |
| TACCACGCGTGATAAGCACA+GGG | + | 10834:4446-4465 | MS.gene66843:intergenic | 50.0% | |
| TCGCTCTCACCAGGAAGAAT+CGG | - | 10834:3356-3375 | MS.gene66843:intron | 50.0% | |
| TGGCCATGGCTGAGACTTTA+TGG | + | 10834:3384-3403 | MS.gene66843:intergenic | 50.0% | |
| ! | CGTTGCTGGTGGTTTTTCTG+CGG | + | 10834:3336-3355 | MS.gene66843:intergenic | 50.0% |
| ! | GTGGGGTGTTTGCTTTGATG+CGG | - | 10834:3977-3996 | MS.gene66843:intron | 50.0% |
| ! | GTTGCTGGTGGTTTTTCTGC+GGG | + | 10834:3335-3354 | MS.gene66843:intergenic | 50.0% |
| !! | GCTCAGGGTCTTATTGCAGA+AGG | - | 10834:4284-4303 | MS.gene66843:intron | 50.0% |
| AACCCTGTGCTTATCACGCG+TGG | - | 10834:4441-4460 | MS.gene66843:CDS | 55.0% | |
| !! | TGGTGTCACTGTTGCCAGAG+AGG | - | 10834:3642-3661 | MS.gene66843:intron | 55.0% |
| CCAGCAACGTCGCTCTCACC+AGG | - | 10834:3347-3366 | MS.gene66843:intron | 65.0% | |
| CCTGGTGAGAGCGACGTTGC+TGG | + | 10834:3350-3369 | MS.gene66843:intergenic | 65.0% | |
| GGTGAGAGCGACGTTGCTGG+TGG | + | 10834:3347-3366 | MS.gene66843:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 10834 | gene | 3262 | 4518 | 3262 | ID=MS.gene66843 |
| 10834 | mRNA | 3262 | 4518 | 3262 | ID=MS.gene66843.t1;Parent=MS.gene66843 |
| 10834 | exon | 4330 | 4518 | 4330 | ID=MS.gene66843.t1.exon1;Parent=MS.gene66843.t1 |
| 10834 | CDS | 4330 | 4518 | 4330 | ID=cds.MS.gene66843.t1;Parent=MS.gene66843.t1 |
| 10834 | exon | 4117 | 4242 | 4117 | ID=MS.gene66843.t1.exon2;Parent=MS.gene66843.t1 |
| 10834 | CDS | 4117 | 4242 | 4117 | ID=cds.MS.gene66843.t1;Parent=MS.gene66843.t1 |
| 10834 | exon | 3467 | 3604 | 3467 | ID=MS.gene66843.t1.exon3;Parent=MS.gene66843.t1 |
| 10834 | CDS | 3467 | 3604 | 3467 | ID=cds.MS.gene66843.t1;Parent=MS.gene66843.t1 |
| 10834 | exon | 3262 | 3357 | 3262 | ID=MS.gene66843.t1.exon4;Parent=MS.gene66843.t1 |
| 10834 | CDS | 3262 | 3357 | 3262 | ID=cds.MS.gene66843.t1;Parent=MS.gene66843.t1 |
| Gene Sequence |
| Protein sequence |
