Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66856.t1 | XP_003605992.2 | 95.8 | 143 | 6 | 0 | 1 | 143 | 1 | 143 | 8.20E-53 | 216.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66856.t1 | Q9SHS7 | 74.7 | 83 | 21 | 0 | 61 | 143 | 66 | 148 | 2.3e-27 | 123.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66856.t1 | G7JET4 | 95.8 | 143 | 6 | 0 | 1 | 143 | 1 | 143 | 5.9e-53 | 216.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050084 | MS.gene66856 | 0.818536 | 1.86E-52 | -1.69E-46 |
| MS.gene050085 | MS.gene66856 | 0.823981 | 1.03E-53 | -1.69E-46 |
| MS.gene052776 | MS.gene66856 | 0.812318 | 4.50E-51 | -1.69E-46 |
| MS.gene056097 | MS.gene66856 | 0.827356 | 1.64E-54 | -1.69E-46 |
| MS.gene059007 | MS.gene66856 | 0.884945 | 1.24E-71 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66856.t1 | MTR_4g050540 | 96.503 | 143 | 5 | 0 | 1 | 143 | 1 | 143 | 1.38e-86 | 272 |
| MS.gene66856.t1 | MTR_2g087530 | 67.785 | 149 | 31 | 2 | 1 | 143 | 1 | 138 | 1.03e-52 | 179 |
| MS.gene66856.t1 | MTR_1g089370 | 54.098 | 61 | 22 | 1 | 69 | 129 | 10 | 64 | 4.86e-14 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66856.t1 | AT1G08420 | 56.970 | 165 | 43 | 5 | 1 | 143 | 1 | 159 | 6.13e-46 | 159 |
| MS.gene66856.t1 | AT1G08420 | 56.970 | 165 | 43 | 5 | 1 | 143 | 1 | 159 | 6.68e-46 | 159 |
| MS.gene66856.t1 | AT2G27210 | 60.135 | 148 | 54 | 1 | 1 | 143 | 1 | 148 | 1.89e-43 | 152 |
| MS.gene66856.t1 | AT2G27210 | 60.135 | 148 | 54 | 1 | 1 | 143 | 1 | 148 | 2.06e-43 | 152 |
| MS.gene66856.t1 | AT4G03080 | 51.667 | 60 | 23 | 1 | 69 | 128 | 10 | 63 | 7.18e-13 | 65.1 |
Find 57 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACAGTTGTGGATGCGATTT+TGG | 0.192831 | 4.4:-51857521 | MS.gene66856:CDS |
| GCGCCGGCGCCGTCGTCTTC+TGG | 0.284104 | 4.4:-51857640 | MS.gene66856:CDS |
| GCAAGGTGCTGCGGCTGGTC+AGG | 0.291963 | 4.4:-51857587 | MS.gene66856:CDS |
| GGCTGTGCCGGCGGTGGGAG+AGG | 0.337377 | 4.4:-51857452 | MS.gene66856:CDS |
| ATTGAAGAGAGAGAACAATT+AGG | 0.371830 | 4.4:-51857679 | MS.gene66856:CDS |
| ATTAGGAGGGAATTCGATTC+CGG | 0.380600 | 4.4:-51857662 | MS.gene66856:CDS |
| GAAGGTACGCCGGGGTATAT+TGG | 0.397267 | 4.4:-51857430 | MS.gene66856:CDS |
| GGTGGTGCGACTGCGCTTGA+AGG | 0.400535 | 4.4:-51857388 | MS.gene66856:CDS |
| GTGGGAGAGGAAGGTACGCC+GGG | 0.406102 | 4.4:-51857439 | MS.gene66856:CDS |
| TGCTGCGATTGTTGCTCCGC+CGG | 0.437457 | 4.4:+51857610 | None:intergenic |
| TCAGGCACTCCTACTTCTGC+TGG | 0.441047 | 4.4:-51857352 | MS.gene66856:CDS |
| GTGCAGCATAGTTCGGTTGT+TGG | 0.448434 | 4.4:-51857565 | MS.gene66856:CDS |
| GAAGGGAATTCAGCGGCGTC+AGG | 0.448903 | 4.4:-51857370 | MS.gene66856:CDS |
| GTGCCGGCGGTGGGAGAGGA+AGG | 0.465868 | 4.4:-51857448 | MS.gene66856:CDS |
| AGGAAGATGGACCGGGGCCG+AGG | 0.468813 | 4.4:-51857492 | MS.gene66856:CDS |
| GTTGACGGCTGTGCCGGCGG+TGG | 0.479291 | 4.4:-51857458 | MS.gene66856:CDS |
| CCTACTTCTGCTGGAAATGC+AGG | 0.480415 | 4.4:-51857343 | MS.gene66856:CDS |
| AGGGAATTCGATTCCGGCGC+CGG | 0.484972 | 4.4:-51857656 | MS.gene66856:CDS |
| GGAGAAGAAGGAAGATGGAC+CGG | 0.490147 | 4.4:-51857500 | MS.gene66856:CDS |
| GAAGACGACGGCGCCGGCGC+CGG | 0.494181 | 4.4:+51857643 | None:intergenic |
| TGGTCAGGTGCAGCATAGTT+CGG | 0.496759 | 4.4:-51857572 | MS.gene66856:CDS |
| CAGCAGCAAGGTGCTGCGGC+TGG | 0.500483 | 4.4:-51857592 | MS.gene66856:CDS |
| GTCGTCTTCTGGTGGATCTC+CGG | 0.516653 | 4.4:-51857629 | MS.gene66856:CDS |
| TCATACGTTGACGGCTGTGC+CGG | 0.516746 | 4.4:-51857464 | MS.gene66856:CDS |
| CCACCAGAAGACGACGGCGC+CGG | 0.519033 | 4.4:+51857637 | None:intergenic |
| GAAGAGAGAGAACAATTAGG+AGG | 0.523063 | 4.4:-51857676 | MS.gene66856:CDS |
| GATGGACCGGGGCCGAGGTG+TGG | 0.525041 | 4.4:-51857487 | MS.gene66856:CDS |
| GAGAAGAAGGAAGATGGACC+GGG | 0.526516 | 4.4:-51857499 | MS.gene66856:CDS |
| GTGGTGCGACTGCGCTTGAA+GGG | 0.527943 | 4.4:-51857387 | MS.gene66856:CDS |
| ATCGCAGCAGCAAGGTGCTG+CGG | 0.528172 | 4.4:-51857596 | MS.gene66856:CDS |
| CCGGCGCCGTCGTCTTCTGG+TGG | 0.534741 | 4.4:-51857637 | MS.gene66856:CDS |
| AAGAGAGAGAACAATTAGGA+GGG | 0.539494 | 4.4:-51857675 | MS.gene66856:CDS |
| TTGACGGCTGTGCCGGCGGT+GGG | 0.546220 | 4.4:-51857457 | MS.gene66856:CDS |
| TGTTGATTCATCGATGGCAT+CGG | 0.563011 | 4.4:-51857731 | MS.gene66856:CDS |
| CCTGCATTTCCAGCAGAAGT+AGG | 0.569856 | 4.4:+51857343 | None:intergenic |
| GGTGGGAGAGGAAGGTACGC+CGG | 0.572703 | 4.4:-51857440 | MS.gene66856:CDS |
| GTATAACTCGGCGCTAGCCT+CGG | 0.574774 | 4.4:+51857541 | None:intergenic |
| TACGTTGACGGCTGTGCCGG+CGG | 0.576135 | 4.4:-51857461 | MS.gene66856:CDS |
| GTATGACCACACCTCGGCCC+CGG | 0.577475 | 4.4:+51857481 | None:intergenic |
| GTACCTTCCTCTCCCACCGC+CGG | 0.599932 | 4.4:+51857445 | None:intergenic |
| GAGCAACAATCGCAGCAGCA+AGG | 0.613703 | 4.4:-51857604 | MS.gene66856:CDS |
| CCACCGAACAAAATCAGCCT+CGG | 0.614940 | 4.4:+51857406 | None:intergenic |
| AATGGATGTTGATTCATCGA+TGG | 0.615102 | 4.4:-51857737 | MS.gene66856:CDS |
| GTCTTCTGGTGGATCTCCGG+CGG | 0.633441 | 4.4:-51857626 | MS.gene66856:CDS |
| GAGGTGTGGTCATACGTTGA+CGG | 0.633733 | 4.4:-51857473 | MS.gene66856:CDS |
| AGCGCCGAGTTATACAGTTG+TGG | 0.637608 | 4.4:-51857533 | MS.gene66856:CDS |
| ATAGTTCGGTTGTTGGACCG+AGG | 0.659224 | 4.4:-51857558 | MS.gene66856:CDS |
| CGCCGGGGTATATTGGTCCG+AGG | 0.664294 | 4.4:-51857423 | MS.gene66856:CDS |
| GCATCCACAACTGTATAACT+CGG | 0.668385 | 4.4:+51857529 | None:intergenic |
| TGCGCTTGAAGGGAATTCAG+CGG | 0.675144 | 4.4:-51857377 | MS.gene66856:CDS |
| GAATGATAACGACTCAACGG+TGG | 0.675250 | 4.4:-51857707 | MS.gene66856:CDS |
| AGCCTCGGACCAATATACCC+CGG | 0.689572 | 4.4:+51857421 | None:intergenic |
| GGAGAATGATAACGACTCAA+CGG | 0.690770 | 4.4:-51857710 | MS.gene66856:CDS |
| GGAGATCCACCAGAAGACGA+CGG | 0.696186 | 4.4:+51857631 | None:intergenic |
| TGGGAGAGGAAGGTACGCCG+GGG | 0.711923 | 4.4:-51857438 | MS.gene66856:CDS |
| AGAAGAAGGAAGATGGACCG+GGG | 0.715571 | 4.4:-51857498 | MS.gene66856:CDS |
| GTCAACGTATGACCACACCT+CGG | 0.753346 | 4.4:+51857475 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGTATTTTACTTTCATGTG+TGG | - | chr4.4:51857717-51857736 | MS.gene66856:CDS | 25.0% |
| ATTGAAGAGAGAGAACAATT+AGG | - | chr4.4:51857351-51857370 | MS.gene66856:CDS | 30.0% | |
| AAGAGAGAGAACAATTAGGA+GGG | - | chr4.4:51857355-51857374 | MS.gene66856:CDS | 35.0% | |
| !! | ATTTTGGAGAAGAAGGAAGA+TGG | - | chr4.4:51857525-51857544 | MS.gene66856:CDS | 35.0% |
| ATTAGGAGGGAATTCGATTC+CGG | - | chr4.4:51857368-51857387 | MS.gene66856:CDS | 40.0% | |
| GAAGAGAGAGAACAATTAGG+AGG | - | chr4.4:51857354-51857373 | MS.gene66856:CDS | 40.0% | |
| GCATCCACAACTGTATAACT+CGG | + | chr4.4:51857504-51857523 | None:intergenic | 40.0% | |
| ! | GGAGAATGATAACGACTCAA+CGG | - | chr4.4:51857320-51857339 | MS.gene66856:intron | 40.0% |
| ! | TACAGTTGTGGATGCGATTT+TGG | - | chr4.4:51857509-51857528 | MS.gene66856:CDS | 40.0% |
| !! | TGTTGATTCATCGATGGCAT+CGG | - | chr4.4:51857299-51857318 | MS.gene66856:intron | 40.0% |
| ! | GAATGATAACGACTCAACGG+TGG | - | chr4.4:51857323-51857342 | MS.gene66856:intron | 45.0% |
| !! | GGATGCGATTTTGGAGAAGA+AGG | - | chr4.4:51857518-51857537 | MS.gene66856:CDS | 45.0% |
| AGAAGAAGGAAGATGGACCG+GGG | - | chr4.4:51857532-51857551 | MS.gene66856:CDS | 50.0% | |
| ATAGTTCGGTTGTTGGACCG+AGG | - | chr4.4:51857472-51857491 | MS.gene66856:CDS | 50.0% | |
| CCACCGAACAAAATCAGCCT+CGG | + | chr4.4:51857627-51857646 | None:intergenic | 50.0% | |
| CCTACTTCTGCTGGAAATGC+AGG | - | chr4.4:51857687-51857706 | MS.gene66856:CDS | 50.0% | |
| CCTGCATTTCCAGCAGAAGT+AGG | + | chr4.4:51857690-51857709 | None:intergenic | 50.0% | |
| GAGAAGAAGGAAGATGGACC+GGG | - | chr4.4:51857531-51857550 | MS.gene66856:CDS | 50.0% | |
| GAGGTGTGGTCATACGTTGA+CGG | - | chr4.4:51857557-51857576 | MS.gene66856:CDS | 50.0% | |
| GGAGAAGAAGGAAGATGGAC+CGG | - | chr4.4:51857530-51857549 | MS.gene66856:CDS | 50.0% | |
| GTCAACGTATGACCACACCT+CGG | + | chr4.4:51857558-51857577 | None:intergenic | 50.0% | |
| GTGCAGCATAGTTCGGTTGT+TGG | - | chr4.4:51857465-51857484 | MS.gene66856:CDS | 50.0% | |
| TGCGCTTGAAGGGAATTCAG+CGG | - | chr4.4:51857653-51857672 | MS.gene66856:CDS | 50.0% | |
| TGGTCAGGTGCAGCATAGTT+CGG | - | chr4.4:51857458-51857477 | MS.gene66856:CDS | 50.0% | |
| ! | AGCGCCGAGTTATACAGTTG+TGG | - | chr4.4:51857497-51857516 | MS.gene66856:CDS | 50.0% |
| !! | GGTCCGAGGCTGATTTTGTT+CGG | - | chr4.4:51857621-51857640 | MS.gene66856:CDS | 50.0% |
| AGCCTCGGACCAATATACCC+CGG | + | chr4.4:51857612-51857631 | None:intergenic | 55.0% | |
| GAGCAACAATCGCAGCAGCA+AGG | - | chr4.4:51857426-51857445 | MS.gene66856:CDS | 55.0% | |
| GGAGATCCACCAGAAGACGA+CGG | + | chr4.4:51857402-51857421 | None:intergenic | 55.0% | |
| TCAGGCACTCCTACTTCTGC+TGG | - | chr4.4:51857678-51857697 | MS.gene66856:CDS | 55.0% | |
| TCATACGTTGACGGCTGTGC+CGG | - | chr4.4:51857566-51857585 | MS.gene66856:CDS | 55.0% | |
| ! | GAAGGTACGCCGGGGTATAT+TGG | - | chr4.4:51857600-51857619 | MS.gene66856:CDS | 55.0% |
| ! | GTATAACTCGGCGCTAGCCT+CGG | + | chr4.4:51857492-51857511 | None:intergenic | 55.0% |
| ! | GTCGTCTTCTGGTGGATCTC+CGG | - | chr4.4:51857401-51857420 | MS.gene66856:CDS | 55.0% |
| !! | CCGAGGCTGATTTTGTTCGG+TGG | - | chr4.4:51857624-51857643 | MS.gene66856:CDS | 55.0% |
| AGGGAATTCGATTCCGGCGC+CGG | - | chr4.4:51857374-51857393 | MS.gene66856:CDS | 60.0% | |
| GAAGGGAATTCAGCGGCGTC+AGG | - | chr4.4:51857660-51857679 | MS.gene66856:CDS | 60.0% | |
| GTGGTGCGACTGCGCTTGAA+GGG | - | chr4.4:51857643-51857662 | MS.gene66856:CDS | 60.0% | |
| TGCTGCGATTGTTGCTCCGC+CGG | + | chr4.4:51857423-51857442 | None:intergenic | 60.0% | |
| ! | GTCTTCTGGTGGATCTCCGG+CGG | - | chr4.4:51857404-51857423 | MS.gene66856:CDS | 60.0% |
| !! | ATCGCAGCAGCAAGGTGCTG+CGG | - | chr4.4:51857434-51857453 | MS.gene66856:CDS | 60.0% |
| CGCCGGGGTATATTGGTCCG+AGG | - | chr4.4:51857607-51857626 | MS.gene66856:CDS | 65.0% | |
| GGTGGTGCGACTGCGCTTGA+AGG | - | chr4.4:51857642-51857661 | MS.gene66856:CDS | 65.0% | |
| GTACCTTCCTCTCCCACCGC+CGG | + | chr4.4:51857588-51857607 | None:intergenic | 65.0% | |
| GTATGACCACACCTCGGCCC+CGG | + | chr4.4:51857552-51857571 | None:intergenic | 65.0% | |
| TACGTTGACGGCTGTGCCGG+CGG | - | chr4.4:51857569-51857588 | MS.gene66856:CDS | 65.0% | |
| ! | GGTGGGAGAGGAAGGTACGC+CGG | - | chr4.4:51857590-51857609 | MS.gene66856:CDS | 65.0% |
| ! | GTGGGAGAGGAAGGTACGCC+GGG | - | chr4.4:51857591-51857610 | MS.gene66856:CDS | 65.0% |
| ! | TGGGAGAGGAAGGTACGCCG+GGG | - | chr4.4:51857592-51857611 | MS.gene66856:CDS | 65.0% |
| AGGAAGATGGACCGGGGCCG+AGG | - | chr4.4:51857538-51857557 | MS.gene66856:CDS | 70.0% | |
| CCACCAGAAGACGACGGCGC+CGG | + | chr4.4:51857396-51857415 | None:intergenic | 70.0% | |
| ! | TTGACGGCTGTGCCGGCGGT+GGG | - | chr4.4:51857573-51857592 | MS.gene66856:CDS | 70.0% |
| !! | CAGCAGCAAGGTGCTGCGGC+TGG | - | chr4.4:51857438-51857457 | MS.gene66856:CDS | 70.0% |
| !! | GCAAGGTGCTGCGGCTGGTC+AGG | - | chr4.4:51857443-51857462 | MS.gene66856:CDS | 70.0% |
| CCGGCGCCGTCGTCTTCTGG+TGG | - | chr4.4:51857393-51857412 | MS.gene66856:CDS | 75.0% | |
| GATGGACCGGGGCCGAGGTG+TGG | - | chr4.4:51857543-51857562 | MS.gene66856:CDS | 75.0% | |
| ! | GTGCCGGCGGTGGGAGAGGA+AGG | - | chr4.4:51857582-51857601 | MS.gene66856:CDS | 75.0% |
| ! | GTTGACGGCTGTGCCGGCGG+TGG | - | chr4.4:51857572-51857591 | MS.gene66856:CDS | 75.0% |
| !! | GCGCCGGCGCCGTCGTCTTC+TGG | - | chr4.4:51857390-51857409 | MS.gene66856:CDS | 80.0% |
| !! | GGCTGTGCCGGCGGTGGGAG+AGG | - | chr4.4:51857578-51857597 | MS.gene66856:CDS | 80.0% |
| !!! | GAAGACGACGGCGCCGGCGC+CGG | + | chr4.4:51857390-51857409 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 51857294 | 51857758 | 51857294 | ID=MS.gene66856 |
| chr4.4 | mRNA | 51857294 | 51857758 | 51857294 | ID=MS.gene66856.t1;Parent=MS.gene66856 |
| chr4.4 | exon | 51857338 | 51857758 | 51857338 | ID=MS.gene66856.t1.exon1;Parent=MS.gene66856.t1 |
| chr4.4 | CDS | 51857338 | 51857758 | 51857338 | ID=cds.MS.gene66856.t1;Parent=MS.gene66856.t1 |
| chr4.4 | exon | 51857294 | 51857304 | 51857294 | ID=MS.gene66856.t1.exon2;Parent=MS.gene66856.t1 |
| chr4.4 | CDS | 51857294 | 51857304 | 51857294 | ID=cds.MS.gene66856.t1;Parent=MS.gene66856.t1 |
| Gene Sequence |
| Protein sequence |
