AlfalfaGEDB | MS.gene66935

M. sativa cultivar XinJiangDaYe / MS.gene66935

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66935.t1	AFK33787.1	100	61	0	0	1	61	420	480	3.40E-24	120.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66935.t1	Q9LME8	55.0	60	27	0	2	61	425	484	7.5e-12	70.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66935.t1	G7KBB5	100.0	61	0	0	1	61	420	480	2.5e-24	120.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052818	MS.gene66935	-0.810125	1.35E-50	-1.69E-46
MS.gene053879	MS.gene66935	0.837608	4.70E-57	-1.69E-46
MS.gene057999	MS.gene66935	0.807961	3.91E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66935.t1	MTR_5g464810	100.000	61	0	0	1	61	420	480	2.20e-35	124
MS.gene66935.t1	MTR_5g064240	100.000	61	0	0	1	61	420	480	2.20e-35	124
MS.gene66935.t1	MTR_0036s0220	90.000	60	6	0	1	60	420	479	1.71e-31	113
MS.gene66935.t1	MTR_5g464760	78.333	60	13	0	1	60	422	481	8.47e-25	94.7
MS.gene66935.t1	MTR_6g014270	52.542	59	27	1	3	61	424	481	1.65e-13	63.2
MS.gene66935.t1	MTR_3g079480	54.386	57	25	1	3	59	434	489	4.93e-13	61.6
MS.gene66935.t1	MTR_2g008210	54.237	59	26	1	2	60	421	478	9.20e-13	60.8
MS.gene66935.t1	MTR_0288s0020	52.542	59	27	1	3	61	406	463	9.28e-13	60.8
MS.gene66935.t1	MTR_6g014220	50.847	59	28	1	3	61	423	480	1.75e-12	60.1
MS.gene66935.t1	MTR_6g014150	52.542	59	27	1	3	61	422	479	2.09e-12	60.1
MS.gene66935.t1	MTR_3g479520	54.386	57	25	1	3	59	428	483	2.20e-12	59.7
MS.gene66935.t1	MTR_6g013920	50.000	60	29	1	2	61	423	481	2.49e-12	59.7
MS.gene66935.t1	MTR_6g013960	49.153	59	29	1	3	61	425	482	2.70e-12	59.7
MS.gene66935.t1	MTR_6g014170	50.000	60	27	2	3	61	407	464	4.99e-12	58.9
MS.gene66935.t1	MTR_5g058320	53.333	60	25	2	3	61	410	467	5.61e-12	58.5
MS.gene66935.t1	MTR_6g013900	48.333	60	30	1	2	61	423	481	7.98e-12	58.2
MS.gene66935.t1	MTR_6g013435	47.458	59	30	1	3	61	252	309	8.25e-12	58.2
MS.gene66935.t1	MTR_6g014310	47.458	59	30	1	3	61	426	483	8.89e-12	58.2
MS.gene66935.t1	MTR_6g014200	49.153	59	29	1	3	61	422	479	1.03e-11	58.2
MS.gene66935.t1	MTR_8g059425	47.458	59	30	1	3	61	423	480	1.24e-11	57.8
MS.gene66935.t1	MTR_6g014050	47.458	59	30	1	3	61	426	483	1.65e-11	57.4
MS.gene66935.t1	MTR_3g479490	50.877	57	27	1	3	59	419	474	2.36e-11	57.0
MS.gene66935.t1	MTR_8g007585	47.458	59	30	1	3	61	337	394	3.23e-11	56.6
MS.gene66935.t1	MTR_6g014187	47.458	59	30	1	3	61	425	482	4.29e-11	56.2
MS.gene66935.t1	MTR_6g013990	50.000	60	27	2	3	61	423	480	4.69e-11	56.2
MS.gene66935.t1	MTR_6g014290	45.763	59	31	1	3	61	423	480	5.70e-11	55.8
MS.gene66935.t1	MTR_6g014185	45.614	57	31	0	3	59	289	345	5.81e-11	55.8
MS.gene66935.t1	MTR_3g479610	52.632	57	26	1	3	59	258	313	9.15e-11	55.1

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66935.t1	AT1G22340	55.000	60	27	2	61	425	484	7.72e-18	75.5
MS.gene66935.t1	AT1G22380	51.724	58	28	3	60	425	482	2.63e-16	70.9
MS.gene66935.t1	AT1G78270	48.276	58	30	2	59	424	481	8.93e-16	69.7
MS.gene66935.t1	AT1G22400	50.000	58	29	3	60	426	483	5.55e-15	67.4
MS.gene66935.t1	AT1G22360	51.724	58	28	3	60	422	479	7.01e-15	67.0

Find 14 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATTCTTGTGAAGGAGTTAA+TGG	0.243787	5.4:-49962045	MS.gene66935:CDS
AGCTTTGCAATGGAAGAAAT+TGG	0.296908	5.4:-49961991	MS.gene66935:CDS
TTGGCACACAATGCTGCTTC+TGG	0.356151	5.4:-49961972	MS.gene66935:CDS
GGAGTTAATGGAAGGAGAAA+AGG	0.396983	5.4:-49962033	MS.gene66935:CDS
AATGCTGCTTCTGGTCCTCA+TGG	0.441237	5.4:-49961963	MS.gene66935:CDS
GAGTTAATGGAAGGAGAAAA+GGG	0.493833	5.4:-49962032	MS.gene66935:CDS
TGGAGATTGAAGATGCTAAG+AGG	0.502096	5.4:-49962079	None:intergenic
ATGCTGCTTCTGGTCCTCAT+GGG	0.522608	5.4:-49961962	MS.gene66935:CDS
TCAAATGTTCACTTCACTTG+AGG	0.545705	5.4:+49961898	None:intergenic
CAAAATAGAGATTCTTGTGA+AGG	0.563473	5.4:-49962054	MS.gene66935:CDS
GGAGATTGAAGATGCTAAGA+GGG	0.565027	5.4:-49962078	None:intergenic
CTTGTGAAGGAGTTAATGGA+AGG	0.576147	5.4:-49962041	MS.gene66935:CDS
TGAGGAGAACATCATGAATG+AGG	0.609158	5.4:+49961916	None:intergenic
TTCATAAATGATGACCCATG+AGG	0.720509	5.4:+49961948	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CAAAATAGAGATTCTTGTGA+AGG	-	chr5.4:49961931-49961950	MS.gene66935:CDS	30.0%
	TGAAAGAAAAAGCTTTGCAA+TGG	-	chr5.4:49961984-49962003	MS.gene66935:CDS	30.0%
	AGCTTTGCAATGGAAGAAAT+TGG	-	chr5.4:49961994-49962013	MS.gene66935:CDS	35.0%
	GAGTTAATGGAAGGAGAAAA+GGG	-	chr5.4:49961953-49961972	MS.gene66935:CDS	35.0%
	GATTCTTGTGAAGGAGTTAA+TGG	-	chr5.4:49961940-49961959	MS.gene66935:CDS	35.0%
	TTCATAAATGATGACCCATG+AGG	+	chr5.4:49962040-49962059	None:intergenic	35.0%
	CTTGTGAAGGAGTTAATGGA+AGG	-	chr5.4:49961944-49961963	MS.gene66935:CDS	40.0%
	GGAGTTAATGGAAGGAGAAA+AGG	-	chr5.4:49961952-49961971	MS.gene66935:CDS	40.0%
	TGAGGAGAACATCATGAATG+AGG	+	chr5.4:49962072-49962091	None:intergenic	40.0%
!	AATGCTGCTTCTGGTCCTCA+TGG	-	chr5.4:49962022-49962041	MS.gene66935:CDS	50.0%
!	ATGCTGCTTCTGGTCCTCAT+GGG	-	chr5.4:49962023-49962042	MS.gene66935:CDS	50.0%
!	TTGGCACACAATGCTGCTTC+TGG	-	chr5.4:49962013-49962032	MS.gene66935:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	49961911	49962096	49961911	ID=MS.gene66935
chr5.4	mRNA	49961911	49962096	49961911	ID=MS.gene66935.t1;Parent=MS.gene66935
chr5.4	exon	49961911	49962096	49961911	ID=MS.gene66935.t1.exon1;Parent=MS.gene66935.t1
chr5.4	CDS	49961911	49962096	49961911	ID=cds.MS.gene66935.t1;Parent=MS.gene66935.t1

Gene Sequence	>MS.gene66935 ATTGAAGATGCTAAGAGGGACAAAATAGAGATTCTTGTGAAGGAGTTAATGGAAGGAGAAAAGGGTAAAGAGATGAAAGAAAAAGCTTTGCAATGGAAGAAATTGGCACACAATGCTGCTTCTGGTCCTCATGGGTCATCATTTATGAATCTTGAGAACCTCATTCATGATGTTCTCCTCAAGTGA
Protein sequence	>MS.gene66935.t1 IEDAKRDKIEILVKELMEGEKGKEMKEKALQWKKLAHNAASGPHGSSFMNLENLIHDVLLK