AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057999


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057999.t1 MTR_4g098710 93.827 81 5 0 1 81 1 81 2.42e-40 127
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 14 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAATTCAGAATCTTATC+TGG 0.320719 8.3:+21879717 None:intergenic
GTGATCAGGATGAACAGTTT+AGG 0.424887 8.3:-21879647 MS.gene057999:CDS
TGTTCATCCTGATCACAGTT+TGG 0.447960 8.3:+21879654 None:intergenic
TGTGTTGCCAAACTGTGATC+AGG 0.505518 8.3:-21879661 MS.gene057999:CDS
TCACTAGACTCCTTCTTGCT+CGG 0.520514 8.3:+21879783 None:intergenic
AAGATCGGATCGATTGCAAT+GGG 0.546672 8.3:-21879582 MS.gene057999:CDS
TCTCAGAGCAAGAAATCAGA+TGG 0.548303 8.3:-21879687 MS.gene057999:CDS
ATCGATTGCAATGGGAGTCT+AGG 0.564890 8.3:-21879574 None:intergenic
CAAGATCGGATCGATTGCAA+TGG 0.580943 8.3:-21879583 MS.gene057999:CDS
GAATCAACTGATGTCAAGAT+CGG 0.588286 8.3:-21879597 MS.gene057999:CDS
CAAGAAGGAGTCTAGTGAAG+TGG 0.591426 8.3:-21879778 MS.gene057999:CDS
TAGCAAGTCACCGAGCAAGA+AGG 0.600583 8.3:-21879793 MS.gene057999:CDS
CTCAGAGCAAGAAATCAGAT+GGG 0.605996 8.3:-21879686 MS.gene057999:CDS
TCAGAGCAAGAAATCAGATG+GGG 0.702066 8.3:-21879685 MS.gene057999:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTTGAATTCAGAATCTTATC+TGG + chr8.3:21879659-21879678 None:intergenic 25.0%
GAATCAACTGATGTCAAGAT+CGG - chr8.3:21879776-21879795 MS.gene057999:CDS 35.0%
AAGATCGGATCGATTGCAAT+GGG - chr8.3:21879791-21879810 MS.gene057999:CDS 40.0%
CTCAGAGCAAGAAATCAGAT+GGG - chr8.3:21879687-21879706 MS.gene057999:CDS 40.0%
GTGATCAGGATGAACAGTTT+AGG - chr8.3:21879726-21879745 MS.gene057999:CDS 40.0%
TCAGAGCAAGAAATCAGATG+GGG - chr8.3:21879688-21879707 MS.gene057999:CDS 40.0%
TCTCAGAGCAAGAAATCAGA+TGG - chr8.3:21879686-21879705 MS.gene057999:CDS 40.0%
TGTTCATCCTGATCACAGTT+TGG + chr8.3:21879722-21879741 None:intergenic 40.0%
CAAGAAGGAGTCTAGTGAAG+TGG - chr8.3:21879595-21879614 MS.gene057999:CDS 45.0%
CAAGATCGGATCGATTGCAA+TGG - chr8.3:21879790-21879809 MS.gene057999:CDS 45.0%
TCACTAGACTCCTTCTTGCT+CGG + chr8.3:21879593-21879612 None:intergenic 45.0%
TGTGTTGCCAAACTGTGATC+AGG - chr8.3:21879712-21879731 MS.gene057999:CDS 45.0%
TAGCAAGTCACCGAGCAAGA+AGG - chr8.3:21879580-21879599 MS.gene057999:CDS 50.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 21879575 21879820 21879575 ID=MS.gene057999
chr8.3 mRNA 21879575 21879820 21879575 ID=MS.gene057999.t1;Parent=MS.gene057999
chr8.3 exon 21879575 21879820 21879575 ID=MS.gene057999.t1.exon1;Parent=MS.gene057999.t1
chr8.3 CDS 21879575 21879820 21879575 ID=cds.MS.gene057999.t1;Parent=MS.gene057999.t1
Gene Sequence

>MS.gene057999

ATGGATAGCAAGTCACCGAGCAAGAAGGAGTCTAGTGAAGTGGCAGCACGCGAGTCGCTTATTGCTATTTCTAACTCTTCACCAGATAAGATTCTGAATTCAAATTCTGTTTCTCAGAGCAAGAAATCAGATGGGGCTGTGTTGCCAAACTGTGATCAGGATGAACAGTTTAGGTCTGAACTGATTTCAATTTCGTATGCTGAATCAACTGATGTCAAGATCGGATCGATTGCAATGGGAGTCTAG

Protein sequence

>MS.gene057999.t1

MDSKSPSKKESSEVAARESLIAISNSSPDKILNSNSVSQSKKSDGAVLPNCDQDEQFRSELISISYAESTDVKIGSIAMGV