Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67114.t1 | AES59462.1 | 97.1 | 70 | 2 | 0 | 1 | 70 | 24 | 93 | 8.70E-32 | 145.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67114.t1 | G7I3M7 | 97.1 | 70 | 2 | 0 | 1 | 70 | 24 | 93 | 6.3e-32 | 145.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053147 | MS.gene67114 | 0.80453 | 2.07E-49 | -1.69E-46 |
| MS.gene053641 | MS.gene67114 | 0.801979 | 6.97E-49 | -1.69E-46 |
| MS.gene05468 | MS.gene67114 | 0.81156 | 6.59E-51 | -1.69E-46 |
| MS.gene054775 | MS.gene67114 | 0.805656 | 1.20E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67114.t1 | MTR_1g019670 | 97.143 | 70 | 2 | 0 | 1 | 70 | 24 | 93 | 4.70e-47 | 144 |
| MS.gene67114.t1 | MTR_1g019640 | 74.286 | 70 | 18 | 0 | 1 | 70 | 25 | 94 | 4.30e-32 | 107 |
| MS.gene67114.t1 | MTR_1g019610 | 72.857 | 70 | 19 | 0 | 1 | 70 | 25 | 94 | 5.37e-32 | 106 |
| MS.gene67114.t1 | MTR_1g019660 | 71.429 | 70 | 20 | 0 | 1 | 70 | 25 | 94 | 1.12e-30 | 103 |
| MS.gene67114.t1 | MTR_1g019600 | 52.381 | 63 | 30 | 0 | 3 | 65 | 32 | 94 | 6.04e-18 | 71.2 |
| MS.gene67114.t1 | MTR_1g019590 | 49.206 | 63 | 32 | 0 | 2 | 64 | 34 | 96 | 1.32e-14 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCTTCTTGTCAAAGGGTT+TGG | 0.309945 | 1.3:-3937239 | MS.gene67114:CDS |
| GATTTACGTTCCTATGTTCC+TGG | 0.332407 | 1.3:-3937347 | MS.gene67114:CDS |
| ACTAATTTGTCCATCATTGT+TGG | 0.414841 | 1.3:+3937289 | None:intergenic |
| AAGGAAAATAATGGAAGTAT+TGG | 0.434553 | 1.3:-3937432 | None:intergenic |
| TTGATTATCTTCTTGTCAAA+GGG | 0.453582 | 1.3:-3937244 | MS.gene67114:CDS |
| GTTGATTATCTTCTTGTCAA+AGG | 0.460253 | 1.3:-3937245 | MS.gene67114:CDS |
| AATATTGATGCCAACAATGA+TGG | 0.465249 | 1.3:-3937299 | MS.gene67114:CDS |
| CAACGCATGGCTCTCCATCC+AGG | 0.488249 | 1.3:+3937329 | None:intergenic |
| TACGTTCCTATGTTCCTGGA+TGG | 0.524072 | 1.3:-3937343 | MS.gene67114:CDS |
| ATGGAGAGCCATGCGTTGTA+TGG | 0.529428 | 1.3:-3937324 | MS.gene67114:CDS |
| AACAATGATGGACAAATTAG+TGG | 0.554056 | 1.3:-3937287 | MS.gene67114:CDS |
| GGCTCTCCATCCAGGAACAT+AGG | 0.575847 | 1.3:+3937337 | None:intergenic |
| AATATTGACCATACAACGCA+TGG | 0.602870 | 1.3:+3937316 | None:intergenic |
| TGATGGACAAATTAGTGGTG+AGG | 0.626914 | 1.3:-3937282 | MS.gene67114:CDS |
| AAAGCAGATAGCAATAAGGA+CGG | 0.681326 | 1.3:-3937407 | MS.gene67114:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTGATTATCTTCTTGTCAAA+GGG | - | chr1.3:3937410-3937429 | MS.gene67114:CDS | 25.0% |
| AATATTGATGCCAACAATGA+TGG | - | chr1.3:3937355-3937374 | MS.gene67114:CDS | 30.0% | |
| GTTGATTATCTTCTTGTCAA+AGG | - | chr1.3:3937409-3937428 | MS.gene67114:CDS | 30.0% | |
| ! | ACTAATTTGTCCATCATTGT+TGG | + | chr1.3:3937368-3937387 | None:intergenic | 30.0% |
| !! | AACAATGATGGACAAATTAG+TGG | - | chr1.3:3937367-3937386 | MS.gene67114:CDS | 30.0% |
| AAAGCAGATAGCAATAAGGA+CGG | - | chr1.3:3937247-3937266 | MS.gene67114:CDS | 35.0% | |
| AATATTGACCATACAACGCA+TGG | + | chr1.3:3937341-3937360 | None:intergenic | 35.0% | |
| GGAAAAAGCAGATAGCAATA+AGG | - | chr1.3:3937243-3937262 | MS.gene67114:CDS | 35.0% | |
| TATCTTCTTGTCAAAGGGTT+TGG | - | chr1.3:3937415-3937434 | MS.gene67114:CDS | 35.0% | |
| GATTTACGTTCCTATGTTCC+TGG | - | chr1.3:3937307-3937326 | MS.gene67114:CDS | 40.0% | |
| ! | TGATGGACAAATTAGTGGTG+AGG | - | chr1.3:3937372-3937391 | MS.gene67114:CDS | 40.0% |
| TACGTTCCTATGTTCCTGGA+TGG | - | chr1.3:3937311-3937330 | MS.gene67114:CDS | 45.0% | |
| ATGGAGAGCCATGCGTTGTA+TGG | - | chr1.3:3937330-3937349 | MS.gene67114:CDS | 50.0% | |
| GGCTCTCCATCCAGGAACAT+AGG | + | chr1.3:3937320-3937339 | None:intergenic | 55.0% | |
| CAACGCATGGCTCTCCATCC+AGG | + | chr1.3:3937328-3937347 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 3937232 | 3937444 | 3937232 | ID=MS.gene67114 |
| chr1.3 | mRNA | 3937232 | 3937444 | 3937232 | ID=MS.gene67114.t1;Parent=MS.gene67114 |
| chr1.3 | exon | 3937232 | 3937444 | 3937232 | ID=MS.gene67114.t1.exon1;Parent=MS.gene67114.t1 |
| chr1.3 | CDS | 3937232 | 3937444 | 3937232 | ID=cds.MS.gene67114.t1;Parent=MS.gene67114.t1 |
| Gene Sequence |
| Protein sequence |
