AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05468


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05468.t1 MTR_6g084590 93.671 79 4 1 1 79 60 137 9.51e-46 143
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGGAGAGGTGGTGATTATA+AGG 0.344076 6.2:+83089349 MS.gene05468:CDS
CCACCACTTTCAGCAGCTTC+CGG 0.372406 6.2:-83089399 None:intergenic
ATTAAGAAGAAGGAAAAGAT+TGG 0.429526 6.2:+83089327 MS.gene05468:CDS
AAGGAAAAGGTGGAAGAGAA+TGG 0.465732 6.2:+83089220 MS.gene05468:CDS
CTGACGACCGCGAGCTCCGC+CGG 0.466025 6.2:-83089261 None:intergenic
TCGTCGCTGACGACCGCGGC+CGG 0.473574 6.2:+83089298 MS.gene05468:CDS
GAGGGAAACTCTTGAGAAAC+CGG 0.490287 6.2:+83089242 MS.gene05468:CDS
GGAAAAGATTGGAGGAGAGG+TGG 0.510074 6.2:+83089338 MS.gene05468:CDS
CTGAAAGTGGTGGCGCGTGA+GGG 0.512091 6.2:+83089409 MS.gene05468:CDS
GCTGAAAGTGGTGGCGCGTG+AGG 0.520422 6.2:+83089408 MS.gene05468:CDS
TTTGATGACAAAGGAAAAGG+TGG 0.521585 6.2:+83089210 MS.gene05468:CDS
CGTCGCTGACGACCGCGGCC+GGG 0.522259 6.2:+83089299 MS.gene05468:CDS
CCGGGAAAATATTAAGAAGA+AGG 0.522927 6.2:+83089317 MS.gene05468:CDS
AAAAGGTGGAAGAGAATGGA+GGG 0.528096 6.2:+83089224 MS.gene05468:CDS
GAAGGAAAAGATTGGAGGAG+AGG 0.529761 6.2:+83089335 MS.gene05468:CDS
AAGAAGAAGGAAAAGATTGG+AGG 0.538627 6.2:+83089330 MS.gene05468:CDS
TGAGAAACCGGCGGAGCTCG+CGG 0.545036 6.2:+83089254 MS.gene05468:CDS
GAAAAGGTGGAAGAGAATGG+AGG 0.573388 6.2:+83089223 MS.gene05468:CDS
GCACCGGAAGCTGCTGAAAG+TGG 0.573462 6.2:+83089396 MS.gene05468:CDS
CCGGAAGCTGCTGAAAGTGG+TGG 0.590260 6.2:+83089399 MS.gene05468:CDS
TGATTTGAATGATGTGGCAC+CGG 0.610457 6.2:+83089380 MS.gene05468:CDS
GATATCGTCGCTGACGACCG+CGG 0.676789 6.2:+83089294 MS.gene05468:CDS
GGAAACTCTTGAGAAACCGG+CGG 0.715924 6.2:+83089245 MS.gene05468:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTAAAATATTTTGATGACAA+AGG + chr6.2:83089201-83089220 MS.gene05468:CDS 20.0%
! ATTAAGAAGAAGGAAAAGAT+TGG + chr6.2:83089327-83089346 MS.gene05468:CDS 25.0%
!!! TATTTTGATGACAAAGGAAA+AGG + chr6.2:83089207-83089226 MS.gene05468:CDS 25.0%
! CCTTCTTCTTAATATTTTCC+CGG - chr6.2:83089320-83089339 None:intergenic 30.0%
!!! AGCTTTTGATTTGAATGATG+TGG + chr6.2:83089374-83089393 MS.gene05468:CDS 30.0%
AAGAAGAAGGAAAAGATTGG+AGG + chr6.2:83089330-83089349 MS.gene05468:CDS 35.0%
CCGGGAAAATATTAAGAAGA+AGG + chr6.2:83089317-83089336 MS.gene05468:CDS 35.0%
! TTTGATGACAAAGGAAAAGG+TGG + chr6.2:83089210-83089229 MS.gene05468:CDS 35.0%
AAAAGGTGGAAGAGAATGGA+GGG + chr6.2:83089224-83089243 MS.gene05468:CDS 40.0%
AAGGAAAAGGTGGAAGAGAA+TGG + chr6.2:83089220-83089239 MS.gene05468:CDS 40.0%
TGATTTGAATGATGTGGCAC+CGG + chr6.2:83089380-83089399 MS.gene05468:CDS 40.0%
GAAAAGGTGGAAGAGAATGG+AGG + chr6.2:83089223-83089242 MS.gene05468:CDS 45.0%
GAAGGAAAAGATTGGAGGAG+AGG + chr6.2:83089335-83089354 MS.gene05468:CDS 45.0%
GAGGAGAGGTGGTGATTATA+AGG + chr6.2:83089349-83089368 MS.gene05468:CDS 45.0%
GAGGGAAACTCTTGAGAAAC+CGG + chr6.2:83089242-83089261 MS.gene05468:CDS 45.0%
! TCTTAATATTTTCCCGGCCG+CGG - chr6.2:83089314-83089333 None:intergenic 45.0%
GGAAAAGATTGGAGGAGAGG+TGG + chr6.2:83089338-83089357 MS.gene05468:CDS 50.0%
GGAAACTCTTGAGAAACCGG+CGG + chr6.2:83089245-83089264 MS.gene05468:CDS 50.0%
CCACCACTTTCAGCAGCTTC+CGG - chr6.2:83089402-83089421 None:intergenic 55.0%
CCGGAAGCTGCTGAAAGTGG+TGG + chr6.2:83089399-83089418 MS.gene05468:CDS 60.0%
GATATCGTCGCTGACGACCG+CGG + chr6.2:83089294-83089313 MS.gene05468:CDS 60.0%
GCACCGGAAGCTGCTGAAAG+TGG + chr6.2:83089396-83089415 MS.gene05468:CDS 60.0%
TGAGAAACCGGCGGAGCTCG+CGG + chr6.2:83089254-83089273 MS.gene05468:CDS 65.0%
CTGACGACCGCGAGCTCCGC+CGG - chr6.2:83089264-83089283 None:intergenic 75.0%
TCGTCGCTGACGACCGCGGC+CGG + chr6.2:83089298-83089317 MS.gene05468:CDS 75.0%
! CGTCGCTGACGACCGCGGCC+GGG + chr6.2:83089299-83089318 MS.gene05468:CDS 80.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 83089189 83089428 83089189 ID=MS.gene05468
chr6.2 mRNA 83089189 83089428 83089189 ID=MS.gene05468.t1;Parent=MS.gene05468
chr6.2 exon 83089189 83089428 83089189 ID=MS.gene05468.t1.exon1;Parent=MS.gene05468.t1
chr6.2 CDS 83089189 83089428 83089189 ID=cds.MS.gene05468.t1;Parent=MS.gene05468.t1
Gene Sequence

>MS.gene05468

ATGAAAGTTGTAGTAAAATATTTTGATGACAAAGGAAAAGGTGGAAGAGAATGGAGGGAAACTCTTGAGAAACCGGCGGAGCTCGCGGTCGTCAGCGACGCAGATGATATCGTCGCTGACGACCGCGGCCGGGAAAATATTAAGAAGAAGGAAAAGATTGGAGGAGAGGTGGTGATTATAAGGCAAGCTTTTGATTTGAATGATGTGGCACCGGAAGCTGCTGAAAGTGGTGGCGCGTGA

Protein sequence

>MS.gene05468.t1

MKVVVKYFDDKGKGGREWRETLEKPAELAVVSDADDIVADDRGRENIKKKEKIGGEVVIIRQAFDLNDVAPEAAESGGA