AlfalfaGEDB | MS.gene67143

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67143.t1	XP_003589173.1	93	86	4	1	1	86	1	84	1.70E-32	148.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67143.t1	G7I3I9	93.0	86	4	1	1	86	1	84	1.2e-32	148.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052665	MS.gene67143	0.817037	4.06E-52	-1.69E-46
MS.gene052732	MS.gene67143	0.834131	3.58E-56	-1.69E-46
MS.gene054550	MS.gene67143	0.804368	2.23E-49	-1.69E-46
MS.gene055469	MS.gene67143	0.800969	1.12E-48	-1.69E-46
MS.gene061006	MS.gene67143	0.804807	1.81E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67143.t1	MTR_1g019280	93.023	86	4	1	1	86	1	84	1.64e-40	140

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67143.t1	AT1G45207	84.211	38	6	0	34	71	21	58	3.47e-14	66.2
MS.gene67143.t1	AT1G45207	84.211	38	6	0	34	71	21	58	3.68e-14	66.2

Find 26 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGTGAGATTTCGGAGTTTA+AGG	0.138254	1.3:-4194348	None:intergenic
TTGTTAACAATGCGTGATTT+AGG	0.191934	1.3:+4194125	None:intergenic
TGTTAACAATGCGTGATTTA+GGG	0.325485	1.3:+4194126	None:intergenic
GTGAAGTACCAGTGAGATTT+CGG	0.355827	1.3:-4194357	None:intergenic
GCCGCCGGTGACGGTGTTGC+CGG	0.428820	1.3:-4194207	None:intergenic
GCGATCGACGCTGGCGGAAG+CGG	0.441021	1.3:-4194291	None:intergenic
AGAGTGAAGATGACGGAGTC+AGG	0.453482	1.3:-4194242	None:intergenic
ACCACCGGCAACACCGTCAC+CGG	0.481138	1.3:+4194203	MS.gene67143:CDS
GGTGTTGCGTGTTCTTGAAC+CGG	0.491640	1.3:-4194183	None:intergenic
AAGCGGAGGAGAAGAGACTA+AGG	0.501003	1.3:-4194274	None:intergenic
GAAGGAGCAGCGATCGACGC+TGG	0.522577	1.3:-4194300	None:intergenic
ATCGACGCTGGCGGAAGCGG+AGG	0.530992	1.3:-4194288	None:intergenic
CTTAAACTCCGAAATCTCAC+TGG	0.541173	1.3:+4194349	MS.gene67143:CDS
TGTATCGCAGCCGCCGGTGA+CGG	0.541960	1.3:-4194216	None:intergenic
GTCGTGAGCATCGGAAGCGA+AGG	0.577090	1.3:-4194318	None:intergenic
GGAGTCGCGGTCGTGAGCAT+CGG	0.579540	1.3:-4194327	None:intergenic
TTCACGAACAACGACGCTCG+TGG	0.590666	1.3:+4194150	MS.gene67143:CDS
AGGACTTGTATCGCAGCCGC+CGG	0.601139	1.3:-4194222	None:intergenic
ACCGGCAACACCGTCACCGG+CGG	0.601674	1.3:+4194206	MS.gene67143:CDS
CGCTCGTGGAGGCGTAACAC+CGG	0.602071	1.3:+4194164	MS.gene67143:CDS
GGAGCAGCGATCGACGCTGG+CGG	0.626239	1.3:-4194297	None:intergenic
TCAAGAACACGCAACACCAC+CGG	0.628130	1.3:+4194188	MS.gene67143:CDS
GCCGGTGACGGTGTTGCCGG+TGG	0.640514	1.3:-4194204	None:intergenic
AGATTCGAGAGTGAAGATGA+CGG	0.670327	1.3:-4194249	None:intergenic
TTTCGGAGTTTAAGGAGTCG+CGG	0.692109	1.3:-4194340	None:intergenic
ACGAACAACGACGCTCGTGG+AGG	0.699696	1.3:+4194153	MS.gene67143:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGATTCGAGAGTGAAGATGA+CGG	-	chr1.3:4194252-4194271	None:intergenic	40.0%
	CAGTGAGATTTCGGAGTTTA+AGG	-	chr1.3:4194351-4194370	None:intergenic	40.0%
	CTTAAACTCCGAAATCTCAC+TGG	+	chr1.3:4194349-4194368	MS.gene67143:CDS	40.0%
!	GTGAAGTACCAGTGAGATTT+CGG	-	chr1.3:4194360-4194379	None:intergenic	40.0%
	TTTCGGAGTTTAAGGAGTCG+CGG	-	chr1.3:4194343-4194362	None:intergenic	45.0%
	AAGCGGAGGAGAAGAGACTA+AGG	-	chr1.3:4194277-4194296	None:intergenic	50.0%
	TCAAGAACACGCAACACCAC+CGG	+	chr1.3:4194188-4194207	MS.gene67143:CDS	50.0%
!	GGTGTTGCGTGTTCTTGAAC+CGG	-	chr1.3:4194186-4194205	None:intergenic	50.0%
!!	AGAGTGAAGATGACGGAGTC+AGG	-	chr1.3:4194245-4194264	None:intergenic	50.0%
	TTCACGAACAACGACGCTCG+TGG	+	chr1.3:4194150-4194169	MS.gene67143:CDS	55.0%
	ACGAACAACGACGCTCGTGG+AGG	+	chr1.3:4194153-4194172	MS.gene67143:CDS	60.0%
	AGGACTTGTATCGCAGCCGC+CGG	-	chr1.3:4194225-4194244	None:intergenic	60.0%
!!	GTCGTGAGCATCGGAAGCGA+AGG	-	chr1.3:4194321-4194340	None:intergenic	60.0%
	ACCACCGGCAACACCGTCAC+CGG	+	chr1.3:4194203-4194222	MS.gene67143:CDS	65.0%
	GAAGGAGCAGCGATCGACGC+TGG	-	chr1.3:4194303-4194322	None:intergenic	65.0%
	GGAGTCGCGGTCGTGAGCAT+CGG	-	chr1.3:4194330-4194349	None:intergenic	65.0%
	TGTATCGCAGCCGCCGGTGA+CGG	-	chr1.3:4194219-4194238	None:intergenic	65.0%
!	CGCTCGTGGAGGCGTAACAC+CGG	+	chr1.3:4194164-4194183	MS.gene67143:CDS	65.0%
	ACCGGCAACACCGTCACCGG+CGG	+	chr1.3:4194206-4194225	MS.gene67143:CDS	70.0%
	ATCGACGCTGGCGGAAGCGG+AGG	-	chr1.3:4194291-4194310	None:intergenic	70.0%
	GCGATCGACGCTGGCGGAAG+CGG	-	chr1.3:4194294-4194313	None:intergenic	70.0%
	GGAGCAGCGATCGACGCTGG+CGG	-	chr1.3:4194300-4194319	None:intergenic	70.0%
!!	GCCGCCGGTGACGGTGTTGC+CGG	-	chr1.3:4194210-4194229	None:intergenic	75.0%
!!	GCCGGTGACGGTGTTGCCGG+TGG	-	chr1.3:4194207-4194226	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	4194134	4194394	4194134	ID=MS.gene67143
chr1.3	mRNA	4194134	4194394	4194134	ID=MS.gene67143.t1;Parent=MS.gene67143
chr1.3	exon	4194134	4194394	4194134	ID=MS.gene67143.t1.exon1;Parent=MS.gene67143.t1
chr1.3	CDS	4194134	4194394	4194134	ID=cds.MS.gene67143.t1;Parent=MS.gene67143.t1

Gene Sequence	>MS.gene67143 ATGCGTGATTTAGGGTTTCACGAACAACGACGCTCGTGGAGGCGTAACACCGGTTCAAGAACACGCAACACCACCGGCAACACCGTCACCGGCGGCTGCGATACAAGTCCTGACTCCGTCATCTTCACTCTCGAATCTAACCTTAGTCTCTTCTCCTCCGCTTCCGCCAGCGTCGATCGCTGCTCCTTCGCTTCCGATGCTCACGACCGCGACTCCTTAAACTCCGAAATCTCACTGGTACTTCACATTGCATTGCACTGA
Protein sequence	>MS.gene67143.t1 MRDLGFHEQRRSWRRNTGSRTRNTTGNTVTGGCDTSPDSVIFTLESNLSLFSSASASVDRCSFASDAHDRDSLNSEISLVLHIALH

M. sativa cultivar XinJiangDaYe / MS.gene67143