Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67260.t1 | RHN56656.1 | 95 | 281 | 13 | 1 | 1 | 280 | 12 | 292 | 4.10E-141 | 510.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67260.t1 | A0A396HTG6 | 95.0 | 281 | 13 | 1 | 1 | 280 | 12 | 292 | 3.0e-141 | 510.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060255 | MS.gene67260 | 0.847577 | 1.06E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67260.t1 | MTR_5g073610 | 94.545 | 275 | 14 | 1 | 1 | 274 | 12 | 286 | 0.0 | 530 |
| MS.gene67260.t1 | MTR_5g073750 | 92.620 | 271 | 19 | 1 | 1 | 271 | 12 | 281 | 2.73e-178 | 493 |
| MS.gene67260.t1 | MTR_5g073810 | 73.913 | 299 | 59 | 1 | 1 | 280 | 12 | 310 | 4.44e-153 | 434 |
| MS.gene67260.t1 | MTR_5g073620 | 57.006 | 314 | 99 | 5 | 1 | 280 | 12 | 323 | 1.54e-105 | 330 |
| MS.gene67260.t1 | MTR_5g073460 | 56.842 | 285 | 116 | 5 | 1 | 280 | 12 | 294 | 7.92e-99 | 304 |
| MS.gene67260.t1 | MTR_5g073480 | 54.545 | 286 | 122 | 6 | 1 | 280 | 12 | 295 | 2.23e-97 | 298 |
| MS.gene67260.t1 | MTR_5g074130 | 53.929 | 280 | 124 | 3 | 1 | 280 | 13 | 287 | 6.79e-94 | 293 |
| MS.gene67260.t1 | MTR_5g073650 | 69.231 | 208 | 45 | 1 | 92 | 280 | 1 | 208 | 7.57e-91 | 272 |
| MS.gene67260.t1 | MTR_5g093530 | 55.128 | 234 | 100 | 3 | 1 | 234 | 13 | 241 | 3.13e-80 | 253 |
| MS.gene67260.t1 | MTR_5g073960 | 46.786 | 280 | 144 | 4 | 1 | 280 | 12 | 286 | 3.33e-77 | 241 |
| MS.gene67260.t1 | MTR_5g074450 | 51.779 | 253 | 100 | 5 | 2 | 234 | 13 | 263 | 1.42e-75 | 239 |
| MS.gene67260.t1 | MTR_5g073920 | 48.057 | 283 | 131 | 5 | 1 | 280 | 12 | 281 | 4.51e-74 | 240 |
| MS.gene67260.t1 | MTR_3g464010 | 45.890 | 292 | 127 | 7 | 1 | 280 | 12 | 284 | 7.63e-74 | 239 |
| MS.gene67260.t1 | MTR_5g073880 | 45.560 | 259 | 116 | 6 | 1 | 258 | 13 | 247 | 3.26e-65 | 205 |
| MS.gene67260.t1 | MTR_5g073660 | 43.554 | 287 | 92 | 6 | 1 | 259 | 31 | 275 | 8.97e-61 | 194 |
| MS.gene67260.t1 | MTR_5g073470 | 42.908 | 282 | 101 | 7 | 1 | 279 | 12 | 236 | 4.59e-60 | 192 |
| MS.gene67260.t1 | MTR_5g074190 | 57.143 | 175 | 70 | 3 | 1 | 175 | 9 | 178 | 1.29e-59 | 197 |
| MS.gene67260.t1 | MTR_5g073820 | 42.509 | 287 | 80 | 5 | 1 | 262 | 31 | 257 | 3.20e-56 | 187 |
| MS.gene67260.t1 | MTR_5g073770 | 39.203 | 301 | 86 | 6 | 1 | 280 | 12 | 236 | 8.39e-53 | 177 |
| MS.gene67260.t1 | MTR_5g073840 | 41.569 | 255 | 132 | 7 | 3 | 247 | 14 | 261 | 7.94e-51 | 168 |
| MS.gene67260.t1 | MTR_5g074150 | 55.000 | 160 | 70 | 2 | 2 | 161 | 24 | 181 | 2.39e-47 | 159 |
| MS.gene67260.t1 | MTR_5g074090 | 51.553 | 161 | 75 | 3 | 2 | 161 | 13 | 171 | 2.59e-45 | 153 |
| MS.gene67260.t1 | MTR_5g074200 | 44.578 | 166 | 90 | 2 | 1 | 166 | 9 | 172 | 2.16e-36 | 132 |
| MS.gene67260.t1 | MTR_5g073860 | 54.310 | 116 | 51 | 2 | 1 | 116 | 26 | 139 | 1.17e-34 | 129 |
| MS.gene67260.t1 | MTR_5g073500 | 48.684 | 152 | 45 | 5 | 2 | 153 | 1 | 119 | 3.10e-29 | 108 |
| MS.gene67260.t1 | MTR_6g078770 | 36.932 | 176 | 110 | 1 | 7 | 182 | 109 | 283 | 2.60e-28 | 111 |
| MS.gene67260.t1 | MTR_6g078610 | 40.506 | 158 | 93 | 1 | 6 | 163 | 593 | 749 | 4.23e-28 | 114 |
| MS.gene67260.t1 | MTR_5g074070 | 34.440 | 241 | 105 | 10 | 3 | 233 | 14 | 211 | 8.05e-28 | 108 |
| MS.gene67260.t1 | MTR_6g075870 | 35.057 | 174 | 108 | 4 | 7 | 177 | 613 | 784 | 9.97e-25 | 103 |
| MS.gene67260.t1 | MTR_5g093540 | 32.143 | 224 | 104 | 8 | 7 | 230 | 15 | 190 | 9.28e-18 | 80.9 |
| MS.gene67260.t1 | MTR_4g023690 | 53.226 | 62 | 28 | 1 | 71 | 132 | 26 | 86 | 3.77e-16 | 72.4 |
| MS.gene67260.t1 | MTR_6g078770 | 28.409 | 176 | 94 | 1 | 7 | 182 | 109 | 252 | 7.79e-15 | 73.6 |
| MS.gene67260.t1 | MTR_6g078620 | 34.437 | 151 | 75 | 3 | 16 | 166 | 94 | 220 | 1.47e-14 | 71.6 |
| MS.gene67260.t1 | MTR_5g073830 | 46.667 | 75 | 40 | 0 | 1 | 75 | 12 | 86 | 1.35e-12 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATTCAGTGGTTCTTAATA+TGG | 0.196083 | 5.1:+56823643 | MS.gene67260:CDS |
| GGATTTGCTGCTGTTGTTGC+TGG | 0.254444 | 5.1:+56823131 | MS.gene67260:CDS |
| TCTTCCTTCAACTATCTCTT+TGG | 0.283998 | 5.1:+56823176 | MS.gene67260:CDS |
| TGTTTCCTCTAGGTCTTTGA+TGG | 0.289475 | 5.1:-56823852 | None:intergenic |
| AAAGTCCATTCAAATGGTTT+TGG | 0.296790 | 5.1:+56823751 | MS.gene67260:CDS |
| TCATACAACATTTGTTGTAT+TGG | 0.324138 | 5.1:+56823313 | MS.gene67260:CDS |
| TAATATGGATTCTCTACAAC+TGG | 0.399665 | 5.1:+56823658 | MS.gene67260:CDS |
| TCCTTCAACTATCTCTTTGG+AGG | 0.401422 | 5.1:+56823179 | MS.gene67260:CDS |
| CAACTGGCTAGTACTAATAT+TGG | 0.425336 | 5.1:+56823674 | MS.gene67260:CDS |
| ATGTGCGTCCTTTGCTGTCA+CGG | 0.435172 | 5.1:+56823409 | MS.gene67260:CDS |
| AGGCTCCAAAACCATTTGAA+TGG | 0.441764 | 5.1:-56823756 | None:intergenic |
| CCCACAAATCTCCACACCTT+TGG | 0.444313 | 5.1:-56823110 | None:intergenic |
| GAACCACTGAATTTCCATCT+TGG | 0.449916 | 5.1:-56823634 | None:intergenic |
| CCAAAGGTGTGGAGATTTGT+GGG | 0.449986 | 5.1:+56823110 | MS.gene67260:CDS |
| AGAGGAAACAACAAATGTTA+TGG | 0.453162 | 5.1:+56823865 | MS.gene67260:CDS |
| AAATTCAAAGTCCATTCAAA+TGG | 0.453874 | 5.1:+56823745 | MS.gene67260:CDS |
| CAACGCGTCAAACATGAAGT+TGG | 0.460762 | 5.1:-56823819 | None:intergenic |
| TAACATTTGTTGTTTCCTCT+AGG | 0.473814 | 5.1:-56823862 | None:intergenic |
| CTAGTACTAATATTGGTAGT+AGG | 0.477743 | 5.1:+56823681 | MS.gene67260:CDS |
| GAAATGAGACTCCATGCATC+TGG | 0.481164 | 5.1:-56823386 | None:intergenic |
| TCCTCCAAAGAGATAGTTGA+AGG | 0.485636 | 5.1:-56823180 | None:intergenic |
| ACACTCACAATCGGTGATAG+AGG | 0.488023 | 5.1:+56823793 | MS.gene67260:CDS |
| ACAAATGTTATGGCGAGGGC+CGG | 0.488972 | 5.1:+56823875 | MS.gene67260:CDS |
| AACGCGTCAAACATGAAGTT+GGG | 0.499337 | 5.1:-56823818 | None:intergenic |
| TCAACTATCTCTTTGGAGGA+TGG | 0.506399 | 5.1:+56823183 | MS.gene67260:CDS |
| CAAATGTTATGGCGAGGGCC+GGG | 0.513454 | 5.1:+56823876 | MS.gene67260:CDS |
| TACTAATATTGGTAGTAGGT+TGG | 0.517377 | 5.1:+56823685 | MS.gene67260:CDS |
| AAAACTTAAACACTCACAAT+CGG | 0.520932 | 5.1:+56823784 | MS.gene67260:CDS |
| GGCAACACTCAAGAAGTCAT+TGG | 0.523682 | 5.1:+56823282 | MS.gene67260:CDS |
| TCTAGCATCTATTGAAGGAA+AGG | 0.524621 | 5.1:-56823588 | None:intergenic |
| TGCATAATACGCCAGATGCA+TGG | 0.531259 | 5.1:+56823375 | MS.gene67260:CDS |
| CAGTGCTCTGAATTCCAAGA+TGG | 0.561368 | 5.1:+56823620 | MS.gene67260:CDS |
| GCCAAAGGTGTGGAGATTTG+TGG | 0.566628 | 5.1:+56823109 | MS.gene67260:CDS |
| AACAACAAATGTTATGGCGA+GGG | 0.585862 | 5.1:+56823871 | MS.gene67260:CDS |
| TCACGGCGCTTAGTTTGTCA+AGG | 0.598752 | 5.1:+56823426 | MS.gene67260:CDS |
| ACTAAGCGCCGTGACAGCAA+AGG | 0.607967 | 5.1:-56823417 | None:intergenic |
| CGTGTGAAGGCACTCGAGCA+CGG | 0.617949 | 5.1:+56823722 | MS.gene67260:CDS |
| AAACAACAAATGTTATGGCG+AGG | 0.620335 | 5.1:+56823870 | MS.gene67260:CDS |
| ATTCCAAGATGGAAATTCAG+TGG | 0.625640 | 5.1:+56823631 | MS.gene67260:CDS |
| AAAACTCGAGACTCGTGTGA+AGG | 0.633334 | 5.1:+56823709 | MS.gene67260:CDS |
| ATGTGTACAACAATAGTCGT+AGG | 0.639424 | 5.1:+56823918 | MS.gene67260:CDS |
| CTATTGAAGGAAAGGATGAA+CGG | 0.640738 | 5.1:-56823580 | None:intergenic |
| CTTTGGAGGATGGACCATGT+TGG | 0.645605 | 5.1:+56823193 | MS.gene67260:CDS |
| GGATGATGCAGCCAAAGGTG+TGG | 0.649228 | 5.1:+56823099 | None:intergenic |
| TCAAGGAACGAAACTCAATG+TGG | 0.661190 | 5.1:+56823443 | MS.gene67260:CDS |
| TGAGTGTTGCCATATCCTTG+CGG | 0.691705 | 5.1:-56823270 | None:intergenic |
| GAGATGGATGATGCAGCCAA+AGG | 0.694750 | 5.1:+56823094 | None:intergenic |
| AGAAACCATCAAAGACCTAG+AGG | 0.695509 | 5.1:+56823847 | MS.gene67260:CDS |
| GTAAAGAGTTATCACCAACA+TGG | 0.757087 | 5.1:-56823207 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GCATTGAAATTATTCATTTT+TGG | + | chr5.1:56823527-56823546 | MS.gene67260:CDS | 20.0% |
| ! | AAAACTTAAACACTCACAAT+CGG | + | chr5.1:56823784-56823803 | MS.gene67260:CDS | 25.0% |
| ! | AAATTCAAAGTCCATTCAAA+TGG | + | chr5.1:56823745-56823764 | MS.gene67260:CDS | 25.0% |
| ! | AAATTCAGTGGTTCTTAATA+TGG | + | chr5.1:56823643-56823662 | MS.gene67260:CDS | 25.0% |
| !! | GATCTTCTATATTTTCTTCT+TGG | + | chr5.1:56823476-56823495 | MS.gene67260:CDS | 25.0% |
| !!! | TCATACAACATTTGTTGTAT+TGG | + | chr5.1:56823313-56823332 | MS.gene67260:CDS | 25.0% |
| AGAGGAAACAACAAATGTTA+TGG | + | chr5.1:56823865-56823884 | MS.gene67260:CDS | 30.0% | |
| GAAAAAATTGTTCTCAAACC+CGG | - | chr5.1:56823897-56823916 | None:intergenic | 30.0% | |
| TAATATGGATTCTCTACAAC+TGG | + | chr5.1:56823658-56823677 | MS.gene67260:CDS | 30.0% | |
| ! | AAAGTCCATTCAAATGGTTT+TGG | + | chr5.1:56823751-56823770 | MS.gene67260:CDS | 30.0% |
| ! | CTAGTACTAATATTGGTAGT+AGG | + | chr5.1:56823681-56823700 | MS.gene67260:CDS | 30.0% |
| ! | TACTAATATTGGTAGTAGGT+TGG | + | chr5.1:56823685-56823704 | MS.gene67260:CDS | 30.0% |
| ! | TTTTCTCTAGCATCTATTGA+AGG | - | chr5.1:56823596-56823615 | None:intergenic | 30.0% |
| !! | GAGTGTTTAAGTTTTCTTAC+AGG | - | chr5.1:56823779-56823798 | None:intergenic | 30.0% |
| !! | TAACATTTGTTGTTTCCTCT+AGG | - | chr5.1:56823865-56823884 | None:intergenic | 30.0% |
| AAACAACAAATGTTATGGCG+AGG | + | chr5.1:56823870-56823889 | MS.gene67260:CDS | 35.0% | |
| AACAACAAATGTTATGGCGA+GGG | + | chr5.1:56823871-56823890 | MS.gene67260:CDS | 35.0% | |
| ATGTGTACAACAATAGTCGT+AGG | + | chr5.1:56823918-56823937 | MS.gene67260:CDS | 35.0% | |
| ATTCCAAGATGGAAATTCAG+TGG | + | chr5.1:56823631-56823650 | MS.gene67260:CDS | 35.0% | |
| CAACTGGCTAGTACTAATAT+TGG | + | chr5.1:56823674-56823693 | MS.gene67260:CDS | 35.0% | |
| CTATTGAAGGAAAGGATGAA+CGG | - | chr5.1:56823583-56823602 | None:intergenic | 35.0% | |
| GTAAAGAGTTATCACCAACA+TGG | - | chr5.1:56823210-56823229 | None:intergenic | 35.0% | |
| TCTAGCATCTATTGAAGGAA+AGG | - | chr5.1:56823591-56823610 | None:intergenic | 35.0% | |
| TCTTCCTTCAACTATCTCTT+TGG | + | chr5.1:56823176-56823195 | MS.gene67260:CDS | 35.0% | |
| !! | TTTTATTTGCCGCAAGGATA+TGG | + | chr5.1:56823261-56823280 | MS.gene67260:CDS | 35.0% |
| !!! | TGTTAGTTTTATTTGCCGCA+AGG | + | chr5.1:56823255-56823274 | MS.gene67260:CDS | 35.0% |
| AACGCGTCAAACATGAAGTT+GGG | - | chr5.1:56823821-56823840 | None:intergenic | 40.0% | |
| AGAAACCATCAAAGACCTAG+AGG | + | chr5.1:56823847-56823866 | MS.gene67260:CDS | 40.0% | |
| AGGCTCCAAAACCATTTGAA+TGG | - | chr5.1:56823759-56823778 | None:intergenic | 40.0% | |
| GAACCACTGAATTTCCATCT+TGG | - | chr5.1:56823637-56823656 | None:intergenic | 40.0% | |
| TCAAGGAACGAAACTCAATG+TGG | + | chr5.1:56823443-56823462 | MS.gene67260:CDS | 40.0% | |
| TCCTCCAAAGAGATAGTTGA+AGG | - | chr5.1:56823183-56823202 | None:intergenic | 40.0% | |
| TCCTTCAACTATCTCTTTGG+AGG | + | chr5.1:56823179-56823198 | MS.gene67260:CDS | 40.0% | |
| TGTTTCCTCTAGGTCTTTGA+TGG | - | chr5.1:56823855-56823874 | None:intergenic | 40.0% | |
| ! | TCAACTATCTCTTTGGAGGA+TGG | + | chr5.1:56823183-56823202 | MS.gene67260:CDS | 40.0% |
| AAAACTCGAGACTCGTGTGA+AGG | + | chr5.1:56823709-56823728 | MS.gene67260:CDS | 45.0% | |
| ACACTCACAATCGGTGATAG+AGG | + | chr5.1:56823793-56823812 | MS.gene67260:CDS | 45.0% | |
| CAACGCGTCAAACATGAAGT+TGG | - | chr5.1:56823822-56823841 | None:intergenic | 45.0% | |
| GAAATGAGACTCCATGCATC+TGG | - | chr5.1:56823389-56823408 | None:intergenic | 45.0% | |
| GGCAACACTCAAGAAGTCAT+TGG | + | chr5.1:56823282-56823301 | MS.gene67260:CDS | 45.0% | |
| TGCATAATACGCCAGATGCA+TGG | + | chr5.1:56823375-56823394 | MS.gene67260:CDS | 45.0% | |
| ! | CAGTGCTCTGAATTCCAAGA+TGG | + | chr5.1:56823620-56823639 | MS.gene67260:CDS | 45.0% |
| ! | CCAAAGGTGTGGAGATTTGT+GGG | + | chr5.1:56823110-56823129 | MS.gene67260:CDS | 45.0% |
| ! | TGAGTGTTGCCATATCCTTG+CGG | - | chr5.1:56823273-56823292 | None:intergenic | 45.0% |
| ACAAATGTTATGGCGAGGGC+CGG | + | chr5.1:56823875-56823894 | MS.gene67260:CDS | 50.0% | |
| CCCACAAATCTCCACACCTT+TGG | - | chr5.1:56823113-56823132 | None:intergenic | 50.0% | |
| ! | ATGTGCGTCCTTTGCTGTCA+CGG | + | chr5.1:56823409-56823428 | MS.gene67260:CDS | 50.0% |
| ! | CTTTGGAGGATGGACCATGT+TGG | + | chr5.1:56823193-56823212 | MS.gene67260:CDS | 50.0% |
| ! | GCCAAAGGTGTGGAGATTTG+TGG | + | chr5.1:56823109-56823128 | MS.gene67260:CDS | 50.0% |
| !! | GGATTTGCTGCTGTTGTTGC+TGG | + | chr5.1:56823131-56823150 | MS.gene67260:CDS | 50.0% |
| !! | TCACGGCGCTTAGTTTGTCA+AGG | + | chr5.1:56823426-56823445 | MS.gene67260:CDS | 50.0% |
| CAAATGTTATGGCGAGGGCC+GGG | + | chr5.1:56823876-56823895 | MS.gene67260:CDS | 55.0% | |
| ! | ACTAAGCGCCGTGACAGCAA+AGG | - | chr5.1:56823420-56823439 | None:intergenic | 55.0% |
| !! | CGTGTGAAGGCACTCGAGCA+CGG | + | chr5.1:56823722-56823741 | MS.gene67260:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 56823101 | 56823943 | 56823101 | ID=MS.gene67260 |
| chr5.1 | mRNA | 56823101 | 56823943 | 56823101 | ID=MS.gene67260.t1;Parent=MS.gene67260 |
| chr5.1 | exon | 56823101 | 56823943 | 56823101 | ID=MS.gene67260.t1.exon1;Parent=MS.gene67260.t1 |
| chr5.1 | CDS | 56823101 | 56823943 | 56823101 | ID=cds.MS.gene67260.t1;Parent=MS.gene67260.t1 |
| Gene Sequence |
| Protein sequence |
