Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060255.t1 | RHN56656.1 | 94 | 281 | 16 | 1 | 1 | 280 | 12 | 292 | 6.00E-140 | 506.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060255.t1 | A0A396HTG6 | 94.0 | 281 | 16 | 1 | 1 | 280 | 12 | 292 | 4.3e-140 | 506.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050780 | MS.gene060255 | 0.805711 | 1.17E-49 | -1.69E-46 |
| MS.gene058426 | MS.gene060255 | 0.801433 | 9.02E-49 | -1.69E-46 |
| MS.gene060255 | MS.gene071463 | 0.804832 | 1.79E-49 | -1.69E-46 |
| MS.gene060255 | MS.gene27500 | 0.815293 | 9.96E-52 | -1.69E-46 |
| MS.gene060255 | MS.gene50951 | 0.801775 | 7.68E-49 | -1.69E-46 |
| MS.gene060255 | MS.gene54314 | 0.832756 | 7.88E-56 | -1.69E-46 |
| MS.gene060255 | MS.gene54316 | 0.839634 | 1.41E-57 | -1.69E-46 |
| MS.gene060255 | MS.gene62931 | 0.823607 | 1.26E-53 | -1.69E-46 |
| MS.gene060255 | MS.gene64443 | 0.835092 | 2.05E-56 | -1.69E-46 |
| MS.gene060255 | MS.gene67260 | 0.847577 | 1.06E-59 | -1.69E-46 |
| MS.gene060255 | MS.gene68819 | 0.802303 | 5.98E-49 | -1.69E-46 |
| MS.gene060255 | MS.gene71493 | 0.81786 | 2.65E-52 | -1.69E-46 |
| MS.gene060255 | MS.gene80682 | 0.8345 | 2.89E-56 | -1.69E-46 |
| MS.gene060255 | MS.gene86035 | 0.80554 | 1.27E-49 | -1.69E-46 |
| MS.gene060255 | MS.gene87685 | 0.814947 | 1.19E-51 | -1.69E-46 |
| MS.gene060255 | MS.gene91372 | 0.801534 | 8.61E-49 | -1.69E-46 |
| MS.gene060255 | MS.gene92763 | 0.824706 | 6.98E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060255.t1 | MTR_5g073610 | 93.455 | 275 | 17 | 1 | 1 | 274 | 12 | 286 | 0.0 | 525 |
| MS.gene060255.t1 | MTR_5g073750 | 91.882 | 271 | 21 | 1 | 1 | 271 | 12 | 281 | 1.32e-176 | 488 |
| MS.gene060255.t1 | MTR_5g073810 | 72.910 | 299 | 62 | 1 | 1 | 280 | 12 | 310 | 3.18e-151 | 429 |
| MS.gene060255.t1 | MTR_5g073620 | 56.688 | 314 | 100 | 5 | 1 | 280 | 12 | 323 | 4.58e-104 | 326 |
| MS.gene060255.t1 | MTR_5g073460 | 56.491 | 285 | 117 | 5 | 1 | 280 | 12 | 294 | 5.25e-98 | 302 |
| MS.gene060255.t1 | MTR_5g073480 | 54.196 | 286 | 123 | 6 | 1 | 280 | 12 | 295 | 1.79e-96 | 296 |
| MS.gene060255.t1 | MTR_5g074130 | 53.571 | 280 | 125 | 3 | 1 | 280 | 13 | 287 | 5.11e-93 | 290 |
| MS.gene060255.t1 | MTR_5g073650 | 68.750 | 208 | 46 | 1 | 92 | 280 | 1 | 208 | 5.14e-90 | 270 |
| MS.gene060255.t1 | MTR_5g093530 | 54.701 | 234 | 101 | 3 | 1 | 234 | 13 | 241 | 2.43e-79 | 250 |
| MS.gene060255.t1 | MTR_5g073960 | 46.786 | 280 | 144 | 4 | 1 | 280 | 12 | 286 | 2.71e-77 | 241 |
| MS.gene060255.t1 | MTR_5g074450 | 51.383 | 253 | 101 | 5 | 2 | 234 | 13 | 263 | 1.98e-74 | 236 |
| MS.gene060255.t1 | MTR_3g464010 | 45.890 | 292 | 127 | 7 | 1 | 280 | 12 | 284 | 1.19e-73 | 238 |
| MS.gene060255.t1 | MTR_5g073920 | 47.350 | 283 | 133 | 5 | 1 | 280 | 12 | 281 | 1.88e-73 | 238 |
| MS.gene060255.t1 | MTR_5g073880 | 45.560 | 259 | 116 | 6 | 1 | 258 | 13 | 247 | 7.04e-65 | 204 |
| MS.gene060255.t1 | MTR_5g073660 | 43.554 | 287 | 92 | 6 | 1 | 259 | 31 | 275 | 9.26e-61 | 194 |
| MS.gene060255.t1 | MTR_5g073470 | 42.908 | 282 | 101 | 7 | 1 | 279 | 12 | 236 | 1.46e-59 | 191 |
| MS.gene060255.t1 | MTR_5g074190 | 57.143 | 175 | 70 | 3 | 1 | 175 | 9 | 178 | 3.20e-59 | 196 |
| MS.gene060255.t1 | MTR_5g073820 | 42.509 | 287 | 80 | 6 | 1 | 262 | 31 | 257 | 2.68e-56 | 188 |
| MS.gene060255.t1 | MTR_5g073770 | 39.931 | 288 | 102 | 6 | 1 | 280 | 12 | 236 | 2.76e-52 | 175 |
| MS.gene060255.t1 | MTR_5g073840 | 48.205 | 195 | 95 | 5 | 1 | 192 | 12 | 203 | 1.45e-50 | 167 |
| MS.gene060255.t1 | MTR_5g074150 | 55.000 | 160 | 70 | 2 | 2 | 161 | 24 | 181 | 5.88e-47 | 157 |
| MS.gene060255.t1 | MTR_5g074090 | 50.932 | 161 | 76 | 3 | 2 | 161 | 13 | 171 | 3.62e-45 | 153 |
| MS.gene060255.t1 | MTR_5g074200 | 35.294 | 238 | 143 | 4 | 1 | 234 | 9 | 239 | 2.45e-36 | 132 |
| MS.gene060255.t1 | MTR_5g073860 | 53.448 | 116 | 52 | 2 | 1 | 116 | 26 | 139 | 1.74e-34 | 128 |
| MS.gene060255.t1 | MTR_5g073500 | 48.684 | 152 | 45 | 5 | 2 | 153 | 1 | 119 | 5.63e-29 | 107 |
| MS.gene060255.t1 | MTR_6g078770 | 37.500 | 176 | 109 | 1 | 7 | 182 | 109 | 283 | 1.23e-28 | 112 |
| MS.gene060255.t1 | MTR_6g078610 | 40.506 | 158 | 93 | 1 | 6 | 163 | 593 | 749 | 8.24e-28 | 112 |
| MS.gene060255.t1 | MTR_5g074070 | 34.025 | 241 | 106 | 10 | 3 | 233 | 14 | 211 | 9.65e-27 | 105 |
| MS.gene060255.t1 | MTR_6g075870 | 34.483 | 174 | 109 | 4 | 7 | 177 | 613 | 784 | 5.97e-24 | 101 |
| MS.gene060255.t1 | MTR_5g093540 | 32.143 | 224 | 104 | 8 | 7 | 230 | 15 | 190 | 2.14e-17 | 79.7 |
| MS.gene060255.t1 | MTR_4g023690 | 53.226 | 62 | 28 | 1 | 71 | 132 | 26 | 86 | 8.87e-16 | 71.2 |
| MS.gene060255.t1 | MTR_6g078620 | 35.099 | 151 | 74 | 3 | 16 | 166 | 94 | 220 | 3.25e-15 | 73.6 |
| MS.gene060255.t1 | MTR_6g078770 | 28.977 | 176 | 93 | 1 | 7 | 182 | 109 | 252 | 3.64e-15 | 74.7 |
| MS.gene060255.t1 | MTR_5g073830 | 45.333 | 75 | 41 | 0 | 1 | 75 | 12 | 86 | 4.88e-12 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATTCAGTGGTTCTTAATA+TGG | 0.196083 | 5.4:-60157037 | MS.gene060255:CDS |
| GGATTTGCTGCTGTTGTTGC+TGG | 0.254444 | 5.4:-60157549 | MS.gene060255:CDS |
| CAACTGGCTAGTACTAATTT+TGG | 0.264131 | 5.4:-60157006 | MS.gene060255:CDS |
| TCTTCCTTCAACTATCTCTT+TGG | 0.283998 | 5.4:-60157504 | MS.gene060255:CDS |
| TGTTTCCTCTAGGTCTTTGA+TGG | 0.289475 | 5.4:+60156828 | None:intergenic |
| AAAGTCCATTCAAATGGTTT+TGG | 0.296790 | 5.4:-60156929 | MS.gene060255:CDS |
| TCATACAACATTTGCTGTAT+TGG | 0.315167 | 5.4:-60157367 | MS.gene060255:CDS |
| TAATATGGATTCTCTACAAC+TGG | 0.399665 | 5.4:-60157022 | MS.gene060255:CDS |
| TCCTTCAACTATCTCTTTGG+AGG | 0.401422 | 5.4:-60157501 | MS.gene060255:CDS |
| ATGTGCATCCTTTGCTGTCA+CGG | 0.423858 | 5.4:-60157271 | MS.gene060255:CDS |
| AGGCTCCAAAACCATTTGAA+TGG | 0.441764 | 5.4:+60156924 | None:intergenic |
| CCCACAAATCTCCACACCTT+TGG | 0.444313 | 5.4:+60157570 | None:intergenic |
| GAACCACTGAATTTCCATCT+TGG | 0.449916 | 5.4:+60157046 | None:intergenic |
| CCAAAGGTGTGGAGATTTGT+GGG | 0.449986 | 5.4:-60157570 | MS.gene060255:CDS |
| AGAGGAAACAACAAATGTTA+TGG | 0.453162 | 5.4:-60156815 | MS.gene060255:CDS |
| AAATTCAAAGTCCATTCAAA+TGG | 0.453874 | 5.4:-60156935 | MS.gene060255:CDS |
| CAACGCGTCAAACATGAAGT+TGG | 0.460762 | 5.4:+60156861 | None:intergenic |
| TAACATTTGTTGTTTCCTCT+AGG | 0.473814 | 5.4:+60156818 | None:intergenic |
| GAAATGAGACTCCATGCATC+TGG | 0.481164 | 5.4:+60157294 | None:intergenic |
| TCCTCCAAAGAGATAGTTGA+AGG | 0.485636 | 5.4:+60157500 | None:intergenic |
| ACACTCACAATCGGTGATAG+AGG | 0.488023 | 5.4:-60156887 | MS.gene060255:CDS |
| ACAAATGTTATGGCGAGGGC+CGG | 0.488972 | 5.4:-60156805 | MS.gene060255:CDS |
| AACGCGTCAAACATGAAGTT+GGG | 0.499337 | 5.4:+60156862 | None:intergenic |
| TCAACTATCTCTTTGGAGGA+TGG | 0.506399 | 5.4:-60157497 | MS.gene060255:CDS |
| CAAATGTTATGGCGAGGGCC+GGG | 0.513454 | 5.4:-60156804 | MS.gene060255:CDS |
| AAAACTTAAACACTCACAAT+CGG | 0.520932 | 5.4:-60156896 | MS.gene060255:CDS |
| GGCAACACTCAAGAAGTCAT+TGG | 0.523682 | 5.4:-60157398 | MS.gene060255:CDS |
| TCTAGCATCTATTGAAGGAA+AGG | 0.524621 | 5.4:+60157092 | None:intergenic |
| TGCATAATACGCCAGATGCA+TGG | 0.531259 | 5.4:-60157305 | MS.gene060255:CDS |
| CAGTGTTCTGAATTCCAAGA+TGG | 0.558001 | 5.4:-60157060 | MS.gene060255:CDS |
| GCCAAAGGTGTGGAGATTTG+TGG | 0.566628 | 5.4:-60157571 | MS.gene060255:CDS |
| AACAACAAATGTTATGGCGA+GGG | 0.585862 | 5.4:-60156809 | MS.gene060255:CDS |
| TCACGGCGCTTAGTTTGTCA+AGG | 0.598752 | 5.4:-60157254 | MS.gene060255:CDS |
| ACTAAGCGCCGTGACAGCAA+AGG | 0.610060 | 5.4:+60157263 | None:intergenic |
| CGTGTGAAGGCACTCGAGCA+CGG | 0.617949 | 5.4:-60156958 | MS.gene060255:CDS |
| AAACAACAAATGTTATGGCG+AGG | 0.620335 | 5.4:-60156810 | MS.gene060255:CDS |
| ATTCCAAGATGGAAATTCAG+TGG | 0.625640 | 5.4:-60157049 | MS.gene060255:CDS |
| AAAACTCGAGACTCGTGTGA+AGG | 0.633334 | 5.4:-60156971 | MS.gene060255:CDS |
| ATGTGTACAACAATAGTCGT+AGG | 0.639424 | 5.4:-60156762 | MS.gene060255:CDS |
| CTATTGAAGGAAAGGATGAA+CGG | 0.640738 | 5.4:+60157100 | None:intergenic |
| CTTTGGAGGATGGACCATGT+TGG | 0.645605 | 5.4:-60157487 | MS.gene060255:CDS |
| GGATGATGCAGCCAAAGGTG+TGG | 0.649228 | 5.4:-60157581 | MS.gene060255:CDS |
| GAGATGGATGATGCAGCCAA+AGG | 0.694750 | 5.4:-60157586 | None:intergenic |
| AGAAACCATCAAAGACCTAG+AGG | 0.695509 | 5.4:-60156833 | MS.gene060255:CDS |
| TGAGTGTTGCCATATCCTTG+CGG | 0.704895 | 5.4:+60157410 | None:intergenic |
| TCAAGGAACGAAACTCAATG+CGG | 0.732487 | 5.4:-60157237 | MS.gene060255:CDS |
| GTAAAGAGTTATCACCAACA+TGG | 0.757087 | 5.4:+60157473 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GCATTGAAATTATTCATTTT+TGG | - | chr5.4:60157185-60157204 | MS.gene060255:CDS | 20.0% |
| ! | AAAACTTAAACACTCACAAT+CGG | - | chr5.4:60157442-60157461 | MS.gene060255:CDS | 25.0% |
| ! | AAATTCAAAGTCCATTCAAA+TGG | - | chr5.4:60157403-60157422 | MS.gene060255:CDS | 25.0% |
| ! | AAATTCAGTGGTTCTTAATA+TGG | - | chr5.4:60157301-60157320 | MS.gene060255:CDS | 25.0% |
| !! | GATCTTCTATATTTTCTTCT+TGG | - | chr5.4:60157134-60157153 | MS.gene060255:CDS | 25.0% |
| AGAGGAAACAACAAATGTTA+TGG | - | chr5.4:60157523-60157542 | MS.gene060255:CDS | 30.0% | |
| GAAAAAATTGTTCTCAAACC+CGG | + | chr5.4:60157555-60157574 | None:intergenic | 30.0% | |
| TAATATGGATTCTCTACAAC+TGG | - | chr5.4:60157316-60157335 | MS.gene060255:CDS | 30.0% | |
| ! | AAAGTCCATTCAAATGGTTT+TGG | - | chr5.4:60157409-60157428 | MS.gene060255:CDS | 30.0% |
| ! | TTTTCTCTAGCATCTATTGA+AGG | + | chr5.4:60157254-60157273 | None:intergenic | 30.0% |
| !! | CTAGTACTAATTTTGGTAGT+AGG | - | chr5.4:60157339-60157358 | MS.gene060255:CDS | 30.0% |
| !! | GAGTGTTTAAGTTTTCTTAC+AGG | + | chr5.4:60157437-60157456 | None:intergenic | 30.0% |
| !! | TAACATTTGTTGTTTCCTCT+AGG | + | chr5.4:60157523-60157542 | None:intergenic | 30.0% |
| !! | TACTAATTTTGGTAGTAGGT+TGG | - | chr5.4:60157343-60157362 | MS.gene060255:CDS | 30.0% |
| !! | TCATACAACATTTGCTGTAT+TGG | - | chr5.4:60156971-60156990 | MS.gene060255:CDS | 30.0% |
| !!! | TGTTAGTTTTATTTTCCGCA+AGG | - | chr5.4:60156913-60156932 | MS.gene060255:CDS | 30.0% |
| !!! | TTTTATTTTCCGCAAGGATA+TGG | - | chr5.4:60156919-60156938 | MS.gene060255:CDS | 30.0% |
| AAACAACAAATGTTATGGCG+AGG | - | chr5.4:60157528-60157547 | MS.gene060255:CDS | 35.0% | |
| AACAACAAATGTTATGGCGA+GGG | - | chr5.4:60157529-60157548 | MS.gene060255:CDS | 35.0% | |
| ATGTGTACAACAATAGTCGT+AGG | - | chr5.4:60157576-60157595 | MS.gene060255:CDS | 35.0% | |
| ATTCCAAGATGGAAATTCAG+TGG | - | chr5.4:60157289-60157308 | MS.gene060255:CDS | 35.0% | |
| CTATTGAAGGAAAGGATGAA+CGG | + | chr5.4:60157241-60157260 | None:intergenic | 35.0% | |
| GTAAAGAGTTATCACCAACA+TGG | + | chr5.4:60156868-60156887 | None:intergenic | 35.0% | |
| TCTAGCATCTATTGAAGGAA+AGG | + | chr5.4:60157249-60157268 | None:intergenic | 35.0% | |
| TCTTCCTTCAACTATCTCTT+TGG | - | chr5.4:60156834-60156853 | MS.gene060255:CDS | 35.0% | |
| ! | CAACTGGCTAGTACTAATTT+TGG | - | chr5.4:60157332-60157351 | MS.gene060255:CDS | 35.0% |
| AACGCGTCAAACATGAAGTT+GGG | + | chr5.4:60157479-60157498 | None:intergenic | 40.0% | |
| AGAAACCATCAAAGACCTAG+AGG | - | chr5.4:60157505-60157524 | MS.gene060255:CDS | 40.0% | |
| AGGCTCCAAAACCATTTGAA+TGG | + | chr5.4:60157417-60157436 | None:intergenic | 40.0% | |
| GAACCACTGAATTTCCATCT+TGG | + | chr5.4:60157295-60157314 | None:intergenic | 40.0% | |
| TCAAGGAACGAAACTCAATG+CGG | - | chr5.4:60157101-60157120 | MS.gene060255:CDS | 40.0% | |
| TCCTCCAAAGAGATAGTTGA+AGG | + | chr5.4:60156841-60156860 | None:intergenic | 40.0% | |
| TCCTTCAACTATCTCTTTGG+AGG | - | chr5.4:60156837-60156856 | MS.gene060255:CDS | 40.0% | |
| TGTTTCCTCTAGGTCTTTGA+TGG | + | chr5.4:60157513-60157532 | None:intergenic | 40.0% | |
| ! | CAGTGTTCTGAATTCCAAGA+TGG | - | chr5.4:60157278-60157297 | MS.gene060255:CDS | 40.0% |
| ! | TCAACTATCTCTTTGGAGGA+TGG | - | chr5.4:60156841-60156860 | MS.gene060255:CDS | 40.0% |
| AAAACTCGAGACTCGTGTGA+AGG | - | chr5.4:60157367-60157386 | MS.gene060255:CDS | 45.0% | |
| ACACTCACAATCGGTGATAG+AGG | - | chr5.4:60157451-60157470 | MS.gene060255:CDS | 45.0% | |
| CAACGCGTCAAACATGAAGT+TGG | + | chr5.4:60157480-60157499 | None:intergenic | 45.0% | |
| GAAATGAGACTCCATGCATC+TGG | + | chr5.4:60157047-60157066 | None:intergenic | 45.0% | |
| GGCAACACTCAAGAAGTCAT+TGG | - | chr5.4:60156940-60156959 | MS.gene060255:CDS | 45.0% | |
| TGCATAATACGCCAGATGCA+TGG | - | chr5.4:60157033-60157052 | MS.gene060255:CDS | 45.0% | |
| ! | ATGTGCATCCTTTGCTGTCA+CGG | - | chr5.4:60157067-60157086 | MS.gene060255:CDS | 45.0% |
| ! | CCAAAGGTGTGGAGATTTGT+GGG | - | chr5.4:60156768-60156787 | MS.gene060255:CDS | 45.0% |
| ! | TGAGTGTTGCCATATCCTTG+CGG | + | chr5.4:60156931-60156950 | None:intergenic | 45.0% |
| ACAAATGTTATGGCGAGGGC+CGG | - | chr5.4:60157533-60157552 | MS.gene060255:CDS | 50.0% | |
| CCCACAAATCTCCACACCTT+TGG | + | chr5.4:60156771-60156790 | None:intergenic | 50.0% | |
| ! | CTTTGGAGGATGGACCATGT+TGG | - | chr5.4:60156851-60156870 | MS.gene060255:CDS | 50.0% |
| ! | GCCAAAGGTGTGGAGATTTG+TGG | - | chr5.4:60156767-60156786 | MS.gene060255:CDS | 50.0% |
| !! | GGATTTGCTGCTGTTGTTGC+TGG | - | chr5.4:60156789-60156808 | MS.gene060255:CDS | 50.0% |
| !! | TCACGGCGCTTAGTTTGTCA+AGG | - | chr5.4:60157084-60157103 | MS.gene060255:CDS | 50.0% |
| CAAATGTTATGGCGAGGGCC+GGG | - | chr5.4:60157534-60157553 | MS.gene060255:CDS | 55.0% | |
| ! | ACTAAGCGCCGTGACAGCAA+AGG | + | chr5.4:60157078-60157097 | None:intergenic | 55.0% |
| !! | CGTGTGAAGGCACTCGAGCA+CGG | - | chr5.4:60157380-60157399 | MS.gene060255:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 60156759 | 60157601 | 60156759 | ID=MS.gene060255 |
| chr5.4 | mRNA | 60156759 | 60157601 | 60156759 | ID=MS.gene060255.t1;Parent=MS.gene060255 |
| chr5.4 | exon | 60156759 | 60157601 | 60156759 | ID=MS.gene060255.t1.exon1;Parent=MS.gene060255.t1 |
| chr5.4 | CDS | 60156759 | 60157601 | 60156759 | ID=cds.MS.gene060255.t1;Parent=MS.gene060255.t1 |
| Gene Sequence |
| Protein sequence |
