Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67305.t1 | XP_013451637.1 | 94.3 | 662 | 36 | 1 | 3 | 664 | 35 | 694 | 0.00E+00 | 1197.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67305.t1 | Q9ZPV5 | 38.0 | 489 | 287 | 7 | 156 | 634 | 129 | 611 | 1.1e-87 | 325.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67305.t1 | A0A072UIM4 | 94.3 | 662 | 36 | 1 | 3 | 664 | 35 | 694 | 0.0e+00 | 1197.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05188 | MS.gene67305 | 0.827711 | 1.35E-54 | -1.69E-46 |
| MS.gene051975 | MS.gene67305 | 0.803182 | 3.94E-49 | -1.69E-46 |
| MS.gene056403 | MS.gene67305 | 0.845052 | 5.16E-59 | -1.69E-46 |
| MS.gene056605 | MS.gene67305 | 0.866519 | 2.69E-65 | -1.69E-46 |
| MS.gene05689 | MS.gene67305 | 0.841258 | 5.30E-58 | -1.69E-46 |
| MS.gene059935 | MS.gene67305 | 0.802025 | 6.82E-49 | -1.69E-46 |
| MS.gene06011 | MS.gene67305 | 0.806299 | 8.80E-50 | -1.69E-46 |
| MS.gene060830 | MS.gene67305 | 0.837013 | 6.68E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67305.t1 | MTR_6g033410 | 96.204 | 685 | 24 | 1 | 5 | 689 | 37 | 719 | 0.0 | 1360 |
| MS.gene67305.t1 | MTR_1g110560 | 36.275 | 510 | 308 | 7 | 134 | 634 | 94 | 595 | 1.20e-102 | 330 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67305.t1 | AT3G55510 | 48.794 | 539 | 253 | 5 | 122 | 652 | 71 | 594 | 2.25e-177 | 518 |
| MS.gene67305.t1 | AT3G55510 | 49.049 | 526 | 248 | 4 | 132 | 652 | 9 | 519 | 5.82e-175 | 509 |
| MS.gene67305.t1 | AT2G18220 | 36.577 | 555 | 319 | 8 | 107 | 634 | 63 | 611 | 6.89e-115 | 363 |
Find 113 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGATCTTCAATGATCTTTC+TGG | 0.132465 | 6.1:-13066821 | MS.gene67305:CDS |
| GGAGTTGCTCTCCTCTTTCC+TGG | 0.215440 | 6.1:-13066142 | MS.gene67305:CDS |
| ACATCAATTGTATTGATCTT+TGG | 0.223877 | 6.1:-13066240 | MS.gene67305:CDS |
| TGAACCTTTGTCTACCATTA+AGG | 0.244522 | 6.1:-13065963 | MS.gene67305:intron |
| TTCAAAGAAGGAAGCTGTTT+TGG | 0.258857 | 6.1:-13066968 | MS.gene67305:CDS |
| TGCAGATTTGTGTTGATCTT+TGG | 0.262550 | 6.1:-13066646 | MS.gene67305:intron |
| CATCAATTGTATTGATCTTT+GGG | 0.276854 | 6.1:-13066239 | MS.gene67305:CDS |
| TCTAGCTTTGCCGATGTTAC+TGG | 0.285191 | 6.1:-13068861 | MS.gene67305:CDS |
| GATGGCAACCCTACTGATTC+TGG | 0.289709 | 6.1:-13063621 | MS.gene67305:CDS |
| TGTGTTGATCTTTGGGTAAC+AGG | 0.293117 | 6.1:-13066638 | MS.gene67305:CDS |
| ATGATTGCTCAAATTATAAA+TGG | 0.309815 | 6.1:-13066163 | MS.gene67305:CDS |
| TGTCGATTCTTTGTGTAAAC+TGG | 0.328689 | 6.1:-13067199 | MS.gene67305:CDS |
| TCTTGAAATCTACAGTTTCC+AGG | 0.333254 | 6.1:-13068476 | MS.gene67305:intron |
| CCTCTTTGTAATCGCTTTCC+TGG | 0.344651 | 6.1:+13064154 | None:intergenic |
| GATGGCAACCCTACTGATTC+CGG | 0.347696 | 6.1:-13063681 | MS.gene67305:CDS |
| CTCTTCTTCTTTCCTTTGCC+CGG | 0.357425 | 6.1:+13063729 | None:intergenic |
| ATCTAAAGAATAAAGCTAAA+TGG | 0.358025 | 6.1:-13066945 | MS.gene67305:CDS |
| GCAGATTTGTGTTGATCTTT+GGG | 0.365083 | 6.1:-13066645 | MS.gene67305:intron |
| TCGTTTGAAACTCTCATTAG+AGG | 0.368893 | 6.1:+13065626 | None:intergenic |
| TAGATGAGACACACTCTTCT+TGG | 0.369973 | 6.1:+13065754 | None:intergenic |
| AGTGGAGCTGTTGCCAGTTC+GGG | 0.371151 | 6.1:+13065678 | None:intergenic |
| CATCCTAATGGCGTTGTTGA+TGG | 0.372443 | 6.1:-13063699 | MS.gene67305:CDS |
| TCTTTGGGTTGCATTTATAT+CGG | 0.374098 | 6.1:-13066224 | MS.gene67305:CDS |
| TCCAGCACGAATGATGTAAC+AGG | 0.376980 | 6.1:+13066043 | None:intergenic |
| GTCTGTCGAAACGTTTCTTC+AGG | 0.390890 | 6.1:-13064367 | MS.gene67305:intron |
| GAGTGGAGCTGTTGCCAGTT+CGG | 0.392086 | 6.1:+13065677 | None:intergenic |
| TATCAAGCGTTTCATTGATC+AGG | 0.396178 | 6.1:-13065601 | MS.gene67305:intron |
| TCCAGGCTTCCCACCATCCT+TGG | 0.398061 | 6.1:+13066000 | None:intergenic |
| CATCCCAATGGCGTAGTTGA+TGG | 0.409710 | 6.1:-13063639 | MS.gene67305:CDS |
| TCATGCGCAAGGCTGCTTCC+AGG | 0.425550 | 6.1:-13064172 | MS.gene67305:CDS |
| TTGCCATCAACAACGCCATT+AGG | 0.426689 | 6.1:+13063696 | None:intergenic |
| ACCAAGGATGGTGGGAAGCC+TGG | 0.430716 | 6.1:-13066001 | MS.gene67305:CDS |
| CTTAAGTATGACTTAATAAG+AGG | 0.433292 | 6.1:+13066910 | None:intergenic |
| ACTGTGGCATGCTGCTCTAT+AGG | 0.441301 | 6.1:+13067131 | None:intergenic |
| TCGACAGACTGCTGATCCTT+CGG | 0.446504 | 6.1:+13064381 | None:intergenic |
| TGCTGATCCTTCGGTGAGAA+AGG | 0.448345 | 6.1:+13064390 | None:intergenic |
| AATGATGAAACTGAGGTTCT+TGG | 0.448944 | 6.1:-13069657 | MS.gene67305:CDS |
| CCCTGTTACATCATTCGTGC+TGG | 0.453632 | 6.1:-13066044 | MS.gene67305:CDS |
| GAGTTGCTCTCCTCTTTCCT+GGG | 0.462179 | 6.1:-13066141 | MS.gene67305:CDS |
| AGGCATTTATGAGACAAGTA+AGG | 0.462603 | 6.1:+13067151 | None:intergenic |
| ACTCTCAGTGGCAAATATCT+AGG | 0.464919 | 6.1:+13066118 | None:intergenic |
| CTCTTATTAAGTCATACTTA+AGG | 0.467323 | 6.1:-13066909 | MS.gene67305:CDS |
| AGAAGCTTGTGCAGTAAAGA+TGG | 0.468827 | 6.1:+13066790 | None:intergenic |
| AAATTTGTATGTGCAGATTG+AGG | 0.475095 | 6.1:+13066500 | None:intergenic |
| ATGAGGCTGATAATATGTTT+AGG | 0.479956 | 6.1:-13067017 | MS.gene67305:CDS |
| GATGAAAATCCCACTAGAAT+CGG | 0.481103 | 6.1:+13066066 | None:intergenic |
| AATTTGTATGTGCAGATTGA+GGG | 0.483627 | 6.1:+13066501 | None:intergenic |
| TAATCTATGCTGCAGTCTCC+GGG | 0.484675 | 6.1:-13063747 | MS.gene67305:intron |
| TCCTTCTTTGAACTCGAGCT+TGG | 0.488161 | 6.1:+13066979 | None:intergenic |
| TGGTTAATAAAATTTGTAGT+TGG | 0.488967 | 6.1:-13066267 | MS.gene67305:CDS |
| TTGCCATCAACTACGCCATT+GGG | 0.491575 | 6.1:+13063636 | None:intergenic |
| AAGAGAAAGCAGCATCCTAA+TGG | 0.491744 | 6.1:-13063711 | MS.gene67305:CDS |
| TTCACTCGTCTGAATGCCGT+TGG | 0.498889 | 6.1:+13067071 | None:intergenic |
| GTTGCCATCAACTACGCCAT+TGG | 0.504206 | 6.1:+13063635 | None:intergenic |
| AATCGTCTTGCCGATTCTAG+TGG | 0.507468 | 6.1:-13066076 | MS.gene67305:CDS |
| CTTGAAATCTACAGTTTCCA+GGG | 0.514226 | 6.1:-13068475 | MS.gene67305:intron |
| AATATCTAGGCCCAGGAAAG+AGG | 0.514834 | 6.1:+13066131 | None:intergenic |
| ATGCTCTGAAAGAAGTTCAA+TGG | 0.518879 | 6.1:+13065731 | None:intergenic |
| CTTGAACAAATTGAAAGAAA+AGG | 0.519699 | 6.1:-13068636 | MS.gene67305:intron |
| ACAAAGCAATGACATGCATA+AGG | 0.523663 | 6.1:-13066424 | MS.gene67305:CDS |
| ACCAAGCTCGAGTTCAAAGA+AGG | 0.525083 | 6.1:-13066980 | MS.gene67305:CDS |
| AGATTGAGGGCGACTGATGA+AGG | 0.526945 | 6.1:+13066514 | None:intergenic |
| ATATCTCAGAGTCAGTGATC+TGG | 0.531688 | 6.1:+13066866 | None:intergenic |
| TAATTCTTTCCAGAATCAGT+AGG | 0.531955 | 6.1:+13063612 | None:intergenic |
| TACAATATTACCAAGGATGG+TGG | 0.536875 | 6.1:-13066010 | MS.gene67305:CDS |
| CATCTATGTGTAGCTCTAAG+TGG | 0.539418 | 6.1:-13066568 | MS.gene67305:CDS |
| ATGCTGCAGTCTCCGGGCAA+AGG | 0.548237 | 6.1:-13063741 | MS.gene67305:intron |
| GATATCTCGTATCATGAGAA+AGG | 0.553297 | 6.1:+13066592 | None:intergenic |
| AGTGGCAAATATCTAGGCCC+AGG | 0.553627 | 6.1:+13066124 | None:intergenic |
| AATTCTTTCCAGAATCAGTA+GGG | 0.559311 | 6.1:+13063613 | None:intergenic |
| ATCGTCTTGCCGATTCTAGT+GGG | 0.560458 | 6.1:-13066075 | MS.gene67305:CDS |
| TAGTGATGGCTTTCTTGAAG+AGG | 0.565826 | 6.1:-13069619 | MS.gene67305:intron |
| TCTAGGTGACATATTGCTGA+AGG | 0.568267 | 6.1:-13066399 | MS.gene67305:CDS |
| TAAGACACTGCCACTGACAC+TGG | 0.571472 | 6.1:+13067095 | None:intergenic |
| CCAGTTCGGGAAAAGATACA+TGG | 0.573824 | 6.1:+13065691 | None:intergenic |
| ATGATGAAAATGCACATACT+CGG | 0.577479 | 6.1:-13067248 | MS.gene67305:CDS |
| ATGACATGCATAAGGCGTCT+AGG | 0.578999 | 6.1:-13066416 | MS.gene67305:CDS |
| TTTACTGCACAAGCTTCTCA+AGG | 0.581430 | 6.1:-13066785 | MS.gene67305:intron |
| GGCATTTATGAGACAAGTAA+GGG | 0.582202 | 6.1:+13067152 | None:intergenic |
| CTACAAAAGCATCATGCGCA+AGG | 0.584364 | 6.1:-13064183 | MS.gene67305:CDS |
| GAATACAATATTACCAAGGA+TGG | 0.584476 | 6.1:-13066013 | MS.gene67305:CDS |
| ACAATATTACCAAGGATGGT+GGG | 0.590717 | 6.1:-13066009 | MS.gene67305:CDS |
| GCCAGAAATTTAGAAAACCC+TGG | 0.594427 | 6.1:+13068458 | None:intergenic |
| TTTATGAGACAAGTAAGGGC+AGG | 0.596259 | 6.1:+13067156 | None:intergenic |
| CTAGGTGACATATTGCTGAA+GGG | 0.596365 | 6.1:-13066398 | MS.gene67305:CDS |
| AGCGATTACAAAGAGGAAGA+AGG | 0.596651 | 6.1:-13064147 | MS.gene67305:intron |
| GAAAATGCACATACTCGGGT+GGG | 0.601534 | 6.1:-13067243 | MS.gene67305:CDS |
| TCTGAAACAACCAGTGTCAG+TGG | 0.602722 | 6.1:-13067105 | MS.gene67305:CDS |
| ATCATCACTTCCAGTAACAT+CGG | 0.602773 | 6.1:+13068851 | None:intergenic |
| AGTGGCAGTGTCTTATCCAA+CGG | 0.603640 | 6.1:-13067087 | MS.gene67305:CDS |
| TAGGTGACATATTGCTGAAG+GGG | 0.605004 | 6.1:-13066397 | MS.gene67305:CDS |
| TGACGTGCCTTTCTCACCGA+AGG | 0.610094 | 6.1:-13064397 | MS.gene67305:CDS |
| TGAAAATGCACATACTCGGG+TGG | 0.615078 | 6.1:-13067244 | MS.gene67305:CDS |
| TAGTGAAAATGATGAAACTG+AGG | 0.617004 | 6.1:-13069664 | MS.gene67305:CDS |
| GTGACATATTGCTGAAGGGG+TGG | 0.621378 | 6.1:-13066394 | MS.gene67305:CDS |
| ATCTTCTTAAGGTAGACGAG+TGG | 0.622146 | 6.1:+13065660 | None:intergenic |
| GGTGATGATTCTGATAGTGA+TGG | 0.623236 | 6.1:-13069633 | MS.gene67305:CDS |
| TCTCGAATACAATATTACCA+AGG | 0.625318 | 6.1:-13066017 | MS.gene67305:CDS |
| GATGAAACTGAGGTTCTTGG+TGG | 0.625727 | 6.1:-13069654 | MS.gene67305:CDS |
| TGATGAAAATGCACATACTC+GGG | 0.632504 | 6.1:-13067247 | MS.gene67305:CDS |
| TGAGCAATCATATACAACAA+AGG | 0.635034 | 6.1:+13066175 | None:intergenic |
| CTTACCTTAATGGTAGACAA+AGG | 0.637847 | 6.1:+13065959 | None:intergenic |
| TCTGAGTCGACAAATTGAGA+AGG | 0.638843 | 6.1:+13066840 | None:intergenic |
| TGAGAGTCAGATGTATCGAA+TGG | 0.639658 | 6.1:-13066102 | MS.gene67305:CDS |
| CCAGCACGAATGATGTAACA+GGG | 0.652463 | 6.1:+13066044 | None:intergenic |
| CCAGGAAAGCGATTACAAAG+AGG | 0.657622 | 6.1:-13064154 | MS.gene67305:CDS |
| TGTTATGATGATGAAGACAC+TGG | 0.662902 | 6.1:-13068434 | MS.gene67305:CDS |
| AAGAAAACACAACATCCCAA+TGG | 0.672006 | 6.1:-13063651 | MS.gene67305:CDS |
| ATTAATGTTCATGCTGCATG+AGG | 0.681258 | 6.1:-13067034 | MS.gene67305:CDS |
| TGACAGGCGGAGAAACGTAG+TGG | 0.691324 | 6.1:-13064227 | MS.gene67305:intron |
| TCGATACATCTGACTCTCAG+TGG | 0.697331 | 6.1:+13066106 | None:intergenic |
| TGGTTGTTTCAGAATCACTG+TGG | 0.767388 | 6.1:+13067115 | None:intergenic |
| GATCTGGTTCAAGAGAAACA+CGG | 0.792315 | 6.1:+13066882 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 13063611 | 13071801 | 13063611 | ID=MS.gene67305 |
| chr6.1 | mRNA | 13063611 | 13071801 | 13063611 | ID=MS.gene67305.t1;Parent=MS.gene67305 |
| chr6.1 | exon | 13071789 | 13071801 | 13071789 | ID=MS.gene67305.t1.exon1;Parent=MS.gene67305.t1 |
| chr6.1 | CDS | 13071789 | 13071801 | 13071789 | ID=cds.MS.gene67305.t1;Parent=MS.gene67305.t1 |
| chr6.1 | exon | 13069620 | 13069792 | 13069620 | ID=MS.gene67305.t1.exon2;Parent=MS.gene67305.t1 |
| chr6.1 | CDS | 13069620 | 13069792 | 13069620 | ID=cds.MS.gene67305.t1;Parent=MS.gene67305.t1 |
| chr6.1 | exon | 13068829 | 13068886 | 13068829 | ID=MS.gene67305.t1.exon3;Parent=MS.gene67305.t1 |
| chr6.1 | CDS | 13068829 | 13068886 | 13068829 | ID=cds.MS.gene67305.t1;Parent=MS.gene67305.t1 |
| chr6.1 | exon | 13068637 | 13068731 | 13068637 | ID=MS.gene67305.t1.exon4;Parent=MS.gene67305.t1 |
| chr6.1 | CDS | 13068637 | 13068731 | 13068637 | ID=cds.MS.gene67305.t1;Parent=MS.gene67305.t1 |
| chr6.1 | exon | 13068412 | 13068483 | 13068412 | ID=MS.gene67305.t1.exon5;Parent=MS.gene67305.t1 |
| chr6.1 | CDS | 13068412 | 13068483 | 13068412 | ID=cds.MS.gene67305.t1;Parent=MS.gene67305.t1 |
| chr6.1 | exon | 13066786 | 13067292 | 13066786 | ID=MS.gene67305.t1.exon6;Parent=MS.gene67305.t1 |
| chr6.1 | CDS | 13066786 | 13067292 | 13066786 | ID=cds.MS.gene67305.t1;Parent=MS.gene67305.t1 |
| chr6.1 | exon | 13066379 | 13066663 | 13066379 | ID=MS.gene67305.t1.exon7;Parent=MS.gene67305.t1 |
| chr6.1 | CDS | 13066379 | 13066663 | 13066379 | ID=cds.MS.gene67305.t1;Parent=MS.gene67305.t1 |
| chr6.1 | exon | 13065964 | 13066293 | 13065964 | ID=MS.gene67305.t1.exon8;Parent=MS.gene67305.t1 |
| chr6.1 | CDS | 13065964 | 13066293 | 13065964 | ID=cds.MS.gene67305.t1;Parent=MS.gene67305.t1 |
| chr6.1 | exon | 13065602 | 13065808 | 13065602 | ID=MS.gene67305.t1.exon9;Parent=MS.gene67305.t1 |
| chr6.1 | CDS | 13065602 | 13065808 | 13065602 | ID=cds.MS.gene67305.t1;Parent=MS.gene67305.t1 |
| chr6.1 | exon | 13064368 | 13064457 | 13064368 | ID=MS.gene67305.t1.exon10;Parent=MS.gene67305.t1 |
| chr6.1 | CDS | 13064368 | 13064457 | 13064368 | ID=cds.MS.gene67305.t1;Parent=MS.gene67305.t1 |
| chr6.1 | exon | 13064148 | 13064243 | 13064148 | ID=MS.gene67305.t1.exon11;Parent=MS.gene67305.t1 |
| chr6.1 | CDS | 13064148 | 13064243 | 13064148 | ID=cds.MS.gene67305.t1;Parent=MS.gene67305.t1 |
| chr6.1 | exon | 13063611 | 13063754 | 13063611 | ID=MS.gene67305.t1.exon12;Parent=MS.gene67305.t1 |
| chr6.1 | CDS | 13063611 | 13063754 | 13063611 | ID=cds.MS.gene67305.t1;Parent=MS.gene67305.t1 |
| Gene Sequence |
| Protein sequence |
