Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67834.t1 | RHN73017.1 | 89.4 | 66 | 6 | 1 | 1 | 65 | 1 | 66 | 6.90E-23 | 115.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67834.t1 | A0A396JCY3 | 89.4 | 66 | 6 | 1 | 1 | 65 | 1 | 66 | 5.0e-23 | 115.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055465 | MS.gene67834 | 0.813495 | 2.49E-51 | -1.69E-46 |
| MS.gene056005 | MS.gene67834 | 0.807544 | 4.80E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67834.t1 | MTR_2g033380 | 88.889 | 63 | 6 | 1 | 1 | 62 | 1 | 63 | 8.69e-35 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGCATGAGATGTTCCTTAT+TGG | 0.244420 | 2.4:-57202671 | None:intergenic |
| TGCATGAGATGTTCCTTATT+GGG | 0.359374 | 2.4:-57202670 | None:intergenic |
| CCTTTGACAAATTTGTCTTT+TGG | 0.367129 | 2.4:-57202292 | None:intergenic |
| ATATGATACAGGTCCCAATA+AGG | 0.444359 | 2.4:+57202657 | MS.gene67834:intron |
| GCAACTTCAGCATTAGTAGC+AGG | 0.456113 | 2.4:+57202805 | MS.gene67834:CDS |
| CCAAAAGACAAATTTGTCAA+AGG | 0.590749 | 2.4:+57202292 | MS.gene67834:CDS |
| GTGGTGATGAACTTGTTGCT+AGG | 0.619962 | 2.4:-57202703 | None:intergenic |
| GAATCTCAAAGGGTTGACCA+TGG | 0.676450 | 2.4:-57202725 | None:intergenic |
| TCTCAAAGGGTTGACCATGG+TGG | 0.686799 | 2.4:-57202722 | None:intergenic |
| CAACAAGTTCATCACCACCA+TGG | 0.713697 | 2.4:+57202708 | MS.gene67834:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAATTTAGCATATAAAAA+AGG | - | chr2.4:57202389-57202408 | None:intergenic | 10.0% |
| !! | GTATATTATAATATAAAGTA+TGG | - | chr2.4:57202472-57202491 | None:intergenic | 10.0% |
| !!! | ATTCTTTAATGTTTTTTAAT+TGG | + | chr2.4:57202558-57202577 | MS.gene67834:intron | 10.0% |
| !! | ATATGCTAAATTAATGTTAA+AGG | + | chr2.4:57202394-57202413 | MS.gene67834:intron | 15.0% |
| !! | TGATGAAAGTAAAATGATTA+TGG | - | chr2.4:57202348-57202367 | None:intergenic | 20.0% |
| !! | ATTGGATTTAAATCTAAAGC+TGG | + | chr2.4:57202576-57202595 | MS.gene67834:intron | 25.0% |
| CCAAAAGACAAATTTGTCAA+AGG | + | chr2.4:57202292-57202311 | MS.gene67834:CDS | 30.0% | |
| GACAAAAAAGAAGAAGACAT+CGG | + | chr2.4:57202751-57202770 | MS.gene67834:CDS | 30.0% | |
| ! | CCTTTGACAAATTTGTCTTT+TGG | - | chr2.4:57202295-57202314 | None:intergenic | 30.0% |
| ! | TTTTGTCACAGAATCTCAAA+GGG | - | chr2.4:57202738-57202757 | None:intergenic | 30.0% |
| ! | TTTTTGTCACAGAATCTCAA+AGG | - | chr2.4:57202739-57202758 | None:intergenic | 30.0% |
| AAAAAAGAAGAAGACATCGG+TGG | + | chr2.4:57202754-57202773 | MS.gene67834:CDS | 35.0% | |
| ATATGATACAGGTCCCAATA+AGG | + | chr2.4:57202657-57202676 | MS.gene67834:intron | 35.0% | |
| ATGCATGAGATGTTCCTTAT+TGG | - | chr2.4:57202674-57202693 | None:intergenic | 35.0% | |
| GAGCATGATTCATATGATAC+AGG | + | chr2.4:57202646-57202665 | MS.gene67834:intron | 35.0% | |
| TGCAGAGAATGAAGAAAGAA+TGG | - | chr2.4:57202614-57202633 | None:intergenic | 35.0% | |
| TGCATGAGATGTTCCTTATT+GGG | - | chr2.4:57202673-57202692 | None:intergenic | 35.0% | |
| ! | ACTACTAGACTTGTTCAATG+TGG | + | chr2.4:57202316-57202335 | MS.gene67834:intron | 35.0% |
| GAGAATGAAGAAAGAATGGC+AGG | - | chr2.4:57202610-57202629 | None:intergenic | 40.0% | |
| CAACAAGTTCATCACCACCA+TGG | + | chr2.4:57202708-57202727 | MS.gene67834:CDS | 45.0% | |
| GAATCTCAAAGGGTTGACCA+TGG | - | chr2.4:57202728-57202747 | None:intergenic | 45.0% | |
| GCAACTTCAGCATTAGTAGC+AGG | + | chr2.4:57202805-57202824 | MS.gene67834:CDS | 45.0% | |
| !! | GTGGTGATGAACTTGTTGCT+AGG | - | chr2.4:57202706-57202725 | None:intergenic | 45.0% |
| TCTCAAAGGGTTGACCATGG+TGG | - | chr2.4:57202725-57202744 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 57202286 | 57202837 | 57202286 | ID=MS.gene67834 |
| chr2.4 | mRNA | 57202286 | 57202837 | 57202286 | ID=MS.gene67834.t1;Parent=MS.gene67834 |
| chr2.4 | exon | 57202286 | 57202313 | 57202286 | ID=MS.gene67834.t1.exon1;Parent=MS.gene67834.t1 |
| chr2.4 | CDS | 57202286 | 57202313 | 57202286 | ID=cds.MS.gene67834.t1;Parent=MS.gene67834.t1 |
| chr2.4 | exon | 57202668 | 57202837 | 57202668 | ID=MS.gene67834.t1.exon2;Parent=MS.gene67834.t1 |
| chr2.4 | CDS | 57202668 | 57202837 | 57202668 | ID=cds.MS.gene67834.t1;Parent=MS.gene67834.t1 |
| Gene Sequence |
| Protein sequence |
