AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056005


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGGCATAGGCGTATTGTTC+TGG 0.295882 1.3:+59735408 None:intergenic
GATTTCTCAAGAAAGGAAAA+AGG 0.302808 1.3:-59736328 MS.gene056005:CDS
ATATAAAGCAGATAAATAAT+TGG 0.326164 1.3:-59735653 MS.gene056005:CDS
ATTTGAATTACCGTGGGATA+AGG 0.413318 1.3:+59736243 None:intergenic
GGATGCAAGAAAAGCATTGA+TGG 0.424199 1.3:-59736292 MS.gene056005:CDS
GGAAAGGTTGAAGCAGTAGA+AGG 0.448898 1.3:-59736452 MS.gene056005:CDS
AAGACAGGATTTCTCAAGAA+AGG 0.449942 1.3:-59736335 MS.gene056005:intron
ATTGGTGGAACGTCCACTAT+AGG 0.452627 1.3:-59736269 MS.gene056005:CDS
GGGTCAGATCATGAGGCAGC+AGG 0.476443 1.3:-59736500 None:intergenic
TTACAGAGGTCCTGCTATGC+TGG 0.477108 1.3:+59735554 None:intergenic
TGGGATAAGGCCACCTATAG+TGG 0.485346 1.3:+59736256 None:intergenic
CAAGAAAAGCATTGATGGAT+TGG 0.506407 1.3:-59736287 MS.gene056005:CDS
GTAAGTAGCACCAGCATAGC+AGG 0.513299 1.3:-59735564 MS.gene056005:CDS
CTTTGTGGCACATCATGTCC+AGG 0.514928 1.3:-59736416 MS.gene056005:CDS
ATAGGCGTATTGTTCTGGAT+TGG 0.551760 1.3:+59735413 None:intergenic
GAAAAGCATTGATGGATTGG+TGG 0.555034 1.3:-59736284 MS.gene056005:CDS
GAAAATCTCATATCTTCAGA+TGG 0.560173 1.3:+59735600 None:intergenic
CTGCAGCTGTCAGATATGAC+TGG 0.564508 1.3:-59735681 MS.gene056005:CDS
TCAGAGGATGAAATGAGTTG+TGG 0.568316 1.3:-59736473 MS.gene056005:CDS
TCAATCAGAGGAAAAGACAC+TGG 0.584588 1.3:-59735626 MS.gene056005:CDS
GGTGGTCATGATGAACTTTG+TGG 0.607949 1.3:-59736431 MS.gene056005:CDS
GGTCAGATCATGAGGCAGCA+GGG 0.615533 1.3:-59736499 None:intergenic
CTATAGGTGGCCTTATCCCA+CGG 0.616947 1.3:-59736253 MS.gene056005:intron
ATAATTGGTTCATCAATCAG+AGG 0.632866 1.3:-59735638 MS.gene056005:CDS
GGTGGAACGTCCACTATAGG+TGG 0.655633 1.3:-59736266 MS.gene056005:CDS
GGATGAAATGAGTTGTGGAA+AGG 0.661174 1.3:-59736468 MS.gene056005:CDS
TGAGGCAGCAGGGACATCAG+AGG 0.661669 1.3:-59736489 MS.gene056005:CDS
TTTGTGGCACATCATGTCCA+GGG 0.672071 1.3:-59736415 MS.gene056005:intron
AAGGTTGAAGCAGTAGAAGG+TGG 0.681013 1.3:-59736449 MS.gene056005:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAAAGCAGATAAATAAT+TGG - chr1.3:59736241-59736260 MS.gene056005:intron 15.0%
!! TTGATTATATATCTGATAAA+AGG - chr1.3:59735953-59735972 MS.gene056005:intron 15.0%
!!! TATAATTTGATGAATTTTTC+AGG - chr1.3:59736179-59736198 MS.gene056005:intron 15.0%
!! TCATCAAATTATAATCTATC+AGG + chr1.3:59736172-59736191 None:intergenic 20.0%
!!! AGATATGAGATTTTCTATTA+TGG - chr1.3:59736302-59736321 MS.gene056005:CDS 20.0%
! AATTTGAATTTGAATTACCG+TGG + chr1.3:59735661-59735680 None:intergenic 25.0%
! ATTTCTCAAGAAAGGAAAAA+GGG - chr1.3:59735567-59735586 MS.gene056005:CDS 25.0%
! ATTTGAATTTGAATTACCGT+GGG + chr1.3:59735660-59735679 None:intergenic 25.0%
!! ATGAGATTTTCTATTATGGA+GGG - chr1.3:59736306-59736325 MS.gene056005:CDS 25.0%
!! TATGAGATTTTCTATTATGG+AGG - chr1.3:59736305-59736324 MS.gene056005:CDS 25.0%
!! TGTTTGTACTAGAAAACTTT+TGG - chr1.3:59736008-59736027 MS.gene056005:intron 25.0%
!!! AATTTGATGAATTTTTCAGG+AGG - chr1.3:59736182-59736201 MS.gene056005:intron 25.0%
ACCAAAAATTATTCCTAGTC+AGG + chr1.3:59735814-59735833 None:intergenic 30.0%
ATATGATATTAGCTAGGCTT+CGG - chr1.3:59736445-59736464 MS.gene056005:CDS 30.0%
GAAAATCTCATATCTTCAGA+TGG + chr1.3:59736297-59736316 None:intergenic 30.0%
GATTTCTCAAGAAAGGAAAA+AGG - chr1.3:59735566-59735585 MS.gene056005:CDS 30.0%
TCATGGATATGATATTAGCT+AGG - chr1.3:59736439-59736458 MS.gene056005:CDS 30.0%
! ATAATTGGTTCATCAATCAG+AGG - chr1.3:59736256-59736275 MS.gene056005:CDS 30.0%
! TCCTGACTAGGAATAATTTT+TGG - chr1.3:59735810-59735829 MS.gene056005:intron 30.0%
! TGTATATTTTGTCCAACGAA+TGG - chr1.3:59735765-59735784 MS.gene056005:intron 30.0%
AAATGCTCCTTCGTAAGTAA+TGG - chr1.3:59735518-59735537 MS.gene056005:intron 35.0%
AAGACAGGATTTCTCAAGAA+AGG - chr1.3:59735559-59735578 MS.gene056005:CDS 35.0%
AATGCTCCTTCGTAAGTAAT+GGG - chr1.3:59735519-59735538 MS.gene056005:intron 35.0%
ACACTGTAACACCAACAAAA+TGG + chr1.3:59735987-59736006 None:intergenic 35.0%
AGCAAAGAAGCTTGACAAAA+TGG + chr1.3:59736119-59736138 None:intergenic 35.0%
ATCCATGACATTCAAGCAAA+AGG + chr1.3:59736427-59736446 None:intergenic 35.0%
ATGGAATTGACGTTGTATCT+TGG + chr1.3:59736100-59736119 None:intergenic 35.0%
ATTTGAATTACCGTGGGATA+AGG + chr1.3:59735654-59735673 None:intergenic 35.0%
CAAATGCAAGTTAAGCTTGT+TGG + chr1.3:59736058-59736077 None:intergenic 35.0%
CCTCTGTAAATTTATTCTGC+AGG - chr1.3:59736354-59736373 MS.gene056005:intron 35.0%
CCTGCAGAATAAATTTACAG+AGG + chr1.3:59736357-59736376 None:intergenic 35.0%
CTGTAAATTTATTCTGCAGG+TGG - chr1.3:59736357-59736376 MS.gene056005:intron 35.0%
GAAAAAGGGTAAACTACCAA+AGG - chr1.3:59735581-59735600 MS.gene056005:CDS 35.0%
TCCATGACATTCAAGCAAAA+GGG + chr1.3:59736426-59736445 None:intergenic 35.0%
TCCTTCATTTGGAAACAGAA+GGG - chr1.3:59735878-59735897 MS.gene056005:intron 35.0%
TTACCTTAGCAACTTAAGAC+AGG - chr1.3:59735544-59735563 MS.gene056005:intron 35.0%
! AATCCTGTCTTAAGTTGCTA+AGG + chr1.3:59735550-59735569 None:intergenic 35.0%
! AATGCTTTTCTTGCATCCTT+TGG + chr1.3:59735600-59735619 None:intergenic 35.0%
! GTACTAGAAAACTTTTGGCA+TGG - chr1.3:59736013-59736032 MS.gene056005:intron 35.0%
! TAACACCAACAAAATGGACT+AGG + chr1.3:59735981-59736000 None:intergenic 35.0%
! TTTGAGTTCAAATCCTGACT+AGG - chr1.3:59735798-59735817 MS.gene056005:intron 35.0%
! TTTTACCTCTCATTGAACTC+CGG - chr1.3:59735842-59735861 MS.gene056005:intron 35.0%
!! CAAGAAAAGCATTGATGGAT+TGG - chr1.3:59735607-59735626 MS.gene056005:CDS 35.0%
!! GCTTAACTTGCATTTGCAAA+AGG - chr1.3:59736062-59736081 MS.gene056005:intron 35.0%
!!! TTGATGAATTTTTCAGGAGG+AGG - chr1.3:59736185-59736204 MS.gene056005:intron 35.0%
AATTTCCTCGAACCATTCGT+TGG + chr1.3:59735780-59735799 None:intergenic 40.0%
ACCCTTCTGTTTCCAAATGA+AGG + chr1.3:59735882-59735901 None:intergenic 40.0%
ATGCTCCTTCGTAAGTAATG+GGG - chr1.3:59735520-59735539 MS.gene056005:intron 40.0%
CTCCTTCATTTGGAAACAGA+AGG - chr1.3:59735877-59735896 MS.gene056005:intron 40.0%
CTTCTTTCGCAGATTAAGAC+AGG + chr1.3:59736399-59736418 None:intergenic 40.0%
GGATGAAATGAGTTGTGGAA+AGG - chr1.3:59735426-59735445 MS.gene056005:CDS 40.0%
TCAATCAGAGGAAAAGACAC+TGG - chr1.3:59736268-59736287 MS.gene056005:CDS 40.0%
TCAGAGGATGAAATGAGTTG+TGG - chr1.3:59735421-59735440 MS.gene056005:CDS 40.0%
TCTTTAAGCTCTTATCACCC+TGG + chr1.3:59735499-59735518 None:intergenic 40.0%
TTCCAAATGAAGGAGACTCT+CGG + chr1.3:59735872-59735891 None:intergenic 40.0%
! ACCCTTTTGCTTGAATGTCA+TGG - chr1.3:59736422-59736441 MS.gene056005:CDS 40.0%
! AGAAAACTTTTGGCATGGTG+AGG - chr1.3:59736018-59736037 MS.gene056005:intron 40.0%
! AGGTTCCTAGTCCATTTTGT+TGG - chr1.3:59735973-59735992 MS.gene056005:intron 40.0%
! ATAGGCGTATTGTTCTGGAT+TGG + chr1.3:59736484-59736503 None:intergenic 40.0%
!! GAAAAGCATTGATGGATTGG+TGG - chr1.3:59735610-59735629 MS.gene056005:CDS 40.0%
!! GGATGCAAGAAAAGCATTGA+TGG - chr1.3:59735602-59735621 MS.gene056005:CDS 40.0%
!! TCTGGATTGGACTTTTGAGA+TGG + chr1.3:59736471-59736490 None:intergenic 40.0%
AAGGTTGAAGCAGTAGAAGG+TGG - chr1.3:59735445-59735464 MS.gene056005:intron 45.0%
ATTGGTGGAACGTCCACTAT+AGG - chr1.3:59735625-59735644 MS.gene056005:CDS 45.0%
GGAAAGGTTGAAGCAGTAGA+AGG - chr1.3:59735442-59735461 MS.gene056005:intron 45.0%
GGTGGTCATGATGAACTTTG+TGG - chr1.3:59735463-59735482 MS.gene056005:intron 45.0%
GTAACCCCCATTACTTACGA+AGG + chr1.3:59735528-59735547 None:intergenic 45.0%
GTAATCCGGAGTTCAATGAG+AGG + chr1.3:59735850-59735869 None:intergenic 45.0%
TACCGAGAGTCTCCTTCATT+TGG - chr1.3:59735867-59735886 MS.gene056005:intron 45.0%
TGCTCCTTCGTAAGTAATGG+GGG - chr1.3:59735521-59735540 MS.gene056005:intron 45.0%
TTTGTCCAACGAATGGTTCG+AGG - chr1.3:59735772-59735791 MS.gene056005:intron 45.0%
TTTGTGGCACATCATGTCCA+GGG - chr1.3:59735479-59735498 MS.gene056005:intron 45.0%
AGGGCATAGGCGTATTGTTC+TGG + chr1.3:59736489-59736508 None:intergenic 50.0%
CTGCAGCTGTCAGATATGAC+TGG - chr1.3:59736213-59736232 MS.gene056005:intron 50.0%
CTTTGTGGCACATCATGTCC+AGG - chr1.3:59735478-59735497 MS.gene056005:intron 50.0%
GAAGGAGACTCTCGGTAATC+CGG + chr1.3:59735864-59735883 None:intergenic 50.0%
GTAAGTAGCACCAGCATAGC+AGG - chr1.3:59736330-59736349 MS.gene056005:CDS 50.0%
TGGGATAAGGCCACCTATAG+TGG + chr1.3:59735641-59735660 None:intergenic 50.0%
TTACAGAGGTCCTGCTATGC+TGG + chr1.3:59736343-59736362 None:intergenic 50.0%
!! CTATAGGTGGCCTTATCCCA+CGG - chr1.3:59735641-59735660 MS.gene056005:CDS 50.0%
GGTGGAACGTCCACTATAGG+TGG - chr1.3:59735628-59735647 MS.gene056005:CDS 55.0%
TGAGGCAGCAGGGACATCAG+AGG - chr1.3:59735405-59735424 MS.gene056005:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 59735401 59736515 59735401 ID=MS.gene056005
chr1.3 mRNA 59735401 59736515 59735401 ID=MS.gene056005.t1;Parent=MS.gene056005
chr1.3 exon 59736416 59736515 59736416 ID=MS.gene056005.t1.exon1;Parent=MS.gene056005.t1
chr1.3 CDS 59736416 59736515 59736416 ID=cds.MS.gene056005.t1;Parent=MS.gene056005.t1
chr1.3 exon 59736254 59736350 59736254 ID=MS.gene056005.t1.exon2;Parent=MS.gene056005.t1
chr1.3 CDS 59736254 59736350 59736254 ID=cds.MS.gene056005.t1;Parent=MS.gene056005.t1
chr1.3 exon 59735558 59735715 59735558 ID=MS.gene056005.t1.exon3;Parent=MS.gene056005.t1
chr1.3 CDS 59735558 59735715 59735558 ID=cds.MS.gene056005.t1;Parent=MS.gene056005.t1
chr1.3 exon 59735401 59735455 59735401 ID=MS.gene056005.t1.exon4;Parent=MS.gene056005.t1
chr1.3 CDS 59735401 59735455 59735401 ID=cds.MS.gene056005.t1;Parent=MS.gene056005.t1
Gene Sequence

>MS.gene056005

ATCATGAGGCAGCAGGGACATCAGAGGATGAAATGAGTTGTGGAAAGGTTGAAGCAGTAGAAGGTGGTCATGATGAACTTTGTGGCACATCATGTCCAGGGTGATAAGAGCTTAAAGAAATGCTCCTTCGTAAGTAATGGGGGTTACCTTAGCAACTTAAGACAGGATTTCTCAAGAAAGGAAAAAGGGTAAACTACCAAAGGATGCAAGAAAAGCATTGATGGATTGGTGGAACGTCCACTATAGGTGGCCTTATCCCACGGTAATTCAAATTCAAATTAATATCACACAAAATATGCTAGCATATATTTTTTTTATATAAGAAAGCATACATATTGTGCAATTAATGTTAACTAAAGCAATATGTATATTTTGTCCAACGAATGGTTCGAGGAAATTTGAGTTCAAATCCTGACTAGGAATAATTTTTGGTCAAACTTTTTTTACCTCTCATTGAACTCCGGATTACCGAGAGTCTCCTTCATTTGGAAACAGAAGGGTTAAGACAAAAAAAGTTGTTTGATTTATAAATATCAAAATCATGTTCTTAACTTGATTATATATCTGATAAAAGGTTCCTAGTCCATTTTGTTGGTGTTACAGTGTGTGTTTGTACTAGAAAACTTTTGGCATGGTGAGGTCTAGATTATGTGTCCAACAAGCTTAACTTGCATTTGCAAAAGGTTGACTTGAACTCCAAGATACAACGTCAATTCCATTTTGTCAAGCTTCTTTGCTCATTTGACATAATATTCAAATTGCTAAAATCCTGATAGATTATAATTTGATGAATTTTTCAGGAGGAGGAGAAACTGCAGCTGTCAGATATGACTGGACTTGATATAAAGCAGATAAATAATTGGTTCATCAATCAGAGGAAAAGACACTGGAAACCATCTGAAGATATGAGATTTTCTATTATGGAGGGTGTAAGTAGCACCAGCATAGCAGGACCTCTGTAAATTTATTCTGCAGGTGGAGTAGAATCGTCGAGTCCTGTCTTAATCTGCGAAAGAAGTTTACCCTTTTGCTTGAATGTCATGGATATGATATTAGCTAGGCTTCGGCCATCTCAAAAGTCCAATCCAGAACAATACGCCTATGCCCTATGTTAA

Protein sequence

>MS.gene056005.t1

IMRQQGHQRMKUVVERLKQUKVVMMNFVAHHVQGFLKKGKRVNYQRMQEKHUWIGGTSTIGGLIPRRRRNCSCQIULDLIUSRUIIGSSIRGKDTGNHLKIUDFLLWRVUVAPAUQDLCFGHLKSPIQNNTPMPYV