Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67990.t1 | XP_028762943.1 | 38.5 | 348 | 53 | 4 | 1 | 187 | 1 | 348 | 2.50E-49 | 205.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67990.t1 | A0A1U8PVE2 | 40.5 | 269 | 64 | 2 | 1 | 173 | 1 | 269 | 2.7e-45 | 191.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050221 | MS.gene67990 | 0.806732 | 7.13E-50 | -1.69E-46 |
| MS.gene058515 | MS.gene67990 | 0.826755 | 2.28E-54 | -1.69E-46 |
| MS.gene059272 | MS.gene67990 | 0.816196 | 6.26E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67990.t1 | MTR_7g062720 | 65.306 | 98 | 34 | 0 | 1 | 98 | 1 | 98 | 1.77e-43 | 142 |
| MS.gene67990.t1 | MTR_5g061150 | 65.432 | 81 | 28 | 0 | 20 | 100 | 10 | 90 | 9.11e-30 | 107 |
| MS.gene67990.t1 | MTR_1g036690 | 58.537 | 82 | 27 | 3 | 107 | 187 | 5 | 80 | 6.67e-24 | 90.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGAGGCTCTTAATATTTC+TGG | 0.080746 | 6.4:-11298893 | MS.gene67990:CDS |
| TTCGGCATTTAAATGAATTT+TGG | 0.166319 | 6.4:+11298841 | None:intergenic |
| CACTTCAAAATAAGGATAAA+AGG | 0.234592 | 6.4:-11297998 | MS.gene67990:CDS |
| CCAACAATCACTTCAAAATA+AGG | 0.244294 | 6.4:-11298006 | MS.gene67990:CDS |
| AAATTGTCCCAAAGATCTTT+TGG | 0.267031 | 6.4:+11298695 | None:intergenic |
| TATTTCTGGAAATAATTATC+TGG | 0.282933 | 6.4:-11298879 | MS.gene67990:CDS |
| TAGAAGATCCAAAAGATCTT+TGG | 0.283634 | 6.4:-11298703 | MS.gene67990:CDS |
| TATAGGTGGGGTTCTATTCC+TGG | 0.331534 | 6.4:+11297844 | None:intergenic |
| TAAAGATCAACAAGATGTTT+TGG | 0.333763 | 6.4:+11298029 | None:intergenic |
| TGAAGATGATTATGGTAATA+TGG | 0.338642 | 6.4:-11297946 | MS.gene67990:CDS |
| CTGGAAATAATTATCTGGCT+TGG | 0.347827 | 6.4:-11298874 | MS.gene67990:CDS |
| TGGAAATAATTATCTGGCTT+GGG | 0.353242 | 6.4:-11298873 | MS.gene67990:CDS |
| AACCATTCATTACGAGCATT+AGG | 0.386252 | 6.4:+11298635 | None:intergenic |
| GAAAATGATGAAGATGATTA+TGG | 0.391347 | 6.4:-11297954 | MS.gene67990:CDS |
| TTTGGGACAATTTAAAAGAA+AGG | 0.395342 | 6.4:-11298685 | MS.gene67990:CDS |
| AGAAGATCCAAAAGATCTTT+GGG | 0.400056 | 6.4:-11298702 | MS.gene67990:CDS |
| TGCTATCGTTGTGGAAGAAA+AGG | 0.423398 | 6.4:-11298077 | MS.gene67990:CDS |
| CCTTCGTCACCACCTTCATA+AGG | 0.433246 | 6.4:-11298750 | MS.gene67990:CDS |
| AGAAATCAGCAGCGTCTAAG+TGG | 0.444453 | 6.4:+11297916 | None:intergenic |
| CAAAACAACTGAACAACAAA+AGG | 0.454927 | 6.4:-11298789 | MS.gene67990:CDS |
| AATTGTATCTCCATGCCCTT+CGG | 0.460742 | 6.4:+11298823 | None:intergenic |
| ACTTCAAAATAAGGATAAAA+GGG | 0.487291 | 6.4:-11297997 | MS.gene67990:CDS |
| CATTTAAATGCCGAAGGGCA+TGG | 0.494088 | 6.4:-11298833 | MS.gene67990:CDS |
| GTTGTGGAAGAAAAGGTCAT+TGG | 0.506394 | 6.4:-11298070 | MS.gene67990:CDS |
| TTCCTAATGCTCGTAATGAA+TGG | 0.506622 | 6.4:-11298637 | MS.gene67990:intron |
| CATTAGAGATGAAAATATCC+AGG | 0.532865 | 6.4:-11297862 | MS.gene67990:CDS |
| CATGGAGATACAATTAAAGA+AGG | 0.533844 | 6.4:-11298815 | MS.gene67990:CDS |
| AAAATTCATTTAAATGCCGA+AGG | 0.537172 | 6.4:-11298839 | MS.gene67990:CDS |
| AACTGAACAACAAAAGGCTA+AGG | 0.582581 | 6.4:-11298783 | MS.gene67990:CDS |
| GAGAGTATTTGCTATCGTTG+TGG | 0.583351 | 6.4:-11298086 | MS.gene67990:CDS |
| ATTCATTACGAGCATTAGGA+AGG | 0.589984 | 6.4:+11298639 | None:intergenic |
| CCTTATGAAGGTGGTGACGA+AGG | 0.598722 | 6.4:+11298750 | None:intergenic |
| GAAATCAGCAGCGTCTAAGT+GGG | 0.605539 | 6.4:+11297917 | None:intergenic |
| AAATTCATTTAAATGCCGAA+GGG | 0.631745 | 6.4:-11298838 | MS.gene67990:CDS |
| GTGGTGACGAAGGAATATCA+TGG | 0.663314 | 6.4:+11298760 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTTGTAAAGAATGATAT+TGG | - | chr6.4:11298646-11298665 | MS.gene67990:CDS | 20.0% |
| !! | ACTTCAAAATAAGGATAAAA+GGG | - | chr6.4:11298777-11298796 | MS.gene67990:CDS | 20.0% |
| !!! | TATTTCTGGAAATAATTATC+TGG | - | chr6.4:11297895-11297914 | MS.gene67990:CDS | 20.0% |
| ! | AAAATTCATTTAAATGCCGA+AGG | - | chr6.4:11297935-11297954 | MS.gene67990:CDS | 25.0% |
| ! | AAATTCATTTAAATGCCGAA+GGG | - | chr6.4:11297936-11297955 | MS.gene67990:CDS | 25.0% |
| ! | CACTTCAAAATAAGGATAAA+AGG | - | chr6.4:11298776-11298795 | MS.gene67990:CDS | 25.0% |
| ! | GAAAATGATGAAGATGATTA+TGG | - | chr6.4:11298820-11298839 | MS.gene67990:CDS | 25.0% |
| ! | TGAAGATGATTATGGTAATA+TGG | - | chr6.4:11298828-11298847 | MS.gene67990:CDS | 25.0% |
| !! | TAAAGATCAACAAGATGTTT+TGG | + | chr6.4:11298748-11298767 | None:intergenic | 25.0% |
| !! | TATTTTCATCTCTAATGAGA+TGG | + | chr6.4:11298909-11298928 | None:intergenic | 25.0% |
| !! | TTACAACATTTTTCATCTTC+TGG | + | chr6.4:11298636-11298655 | None:intergenic | 25.0% |
| !! | TTCGGCATTTAAATGAATTT+TGG | + | chr6.4:11297936-11297955 | None:intergenic | 25.0% |
| !! | TTTGGGACAATTTAAAAGAA+AGG | - | chr6.4:11298089-11298108 | MS.gene67990:CDS | 25.0% |
| !!! | CATTTTTTAGATCCTTATGA+AGG | + | chr6.4:11298039-11298058 | None:intergenic | 25.0% |
| !!! | GAGAAAATGTGTTTTTGTTT+TGG | + | chr6.4:11298612-11298631 | None:intergenic | 25.0% |
| AGAAGATCCAAAAGATCTTT+GGG | - | chr6.4:11298072-11298091 | MS.gene67990:CDS | 30.0% | |
| ATGGTTATATTTGCGTTTAC+AGG | - | chr6.4:11298156-11298175 | MS.gene67990:intron | 30.0% | |
| CAAAACAACTGAACAACAAA+AGG | - | chr6.4:11297985-11298004 | MS.gene67990:CDS | 30.0% | |
| CATGGAGATACAATTAAAGA+AGG | - | chr6.4:11297959-11297978 | MS.gene67990:CDS | 30.0% | |
| CATTAGAGATGAAAATATCC+AGG | - | chr6.4:11298912-11298931 | MS.gene67990:CDS | 30.0% | |
| CCAACAATCACTTCAAAATA+AGG | - | chr6.4:11298768-11298787 | MS.gene67990:CDS | 30.0% | |
| GTCTGTAATCGAATATAACT+CGG | - | chr6.4:11298186-11298205 | MS.gene67990:intron | 30.0% | |
| TACATTAGATGCATGAAAAG+TGG | + | chr6.4:11298290-11298309 | None:intergenic | 30.0% | |
| TAGAAGATCCAAAAGATCTT+TGG | - | chr6.4:11298071-11298090 | MS.gene67990:CDS | 30.0% | |
| ! | AAATTGTCCCAAAGATCTTT+TGG | + | chr6.4:11298082-11298101 | None:intergenic | 30.0% |
| ! | AAGATCTTTTGGATCTTCTA+TGG | + | chr6.4:11298071-11298090 | None:intergenic | 30.0% |
| ! | GCTTTTGATGAAAAATCACT+CGG | - | chr6.4:11298396-11298415 | MS.gene67990:intron | 30.0% |
| ! | TGGAAATAATTATCTGGCTT+GGG | - | chr6.4:11297901-11297920 | MS.gene67990:CDS | 30.0% |
| ! | TTTGAGGCTCTTAATATTTC+TGG | - | chr6.4:11297881-11297900 | MS.gene67990:CDS | 30.0% |
| ! | TTTTTAGATCCTTATGAAGG+TGG | + | chr6.4:11298036-11298055 | None:intergenic | 30.0% |
| !! | GGTTTTAATCGTAATCACAA+TGG | - | chr6.4:11298562-11298581 | MS.gene67990:intron | 30.0% |
| !!! | CCTTATTTTGAAGTGATTGT+TGG | + | chr6.4:11298771-11298790 | None:intergenic | 30.0% |
| AACCATTCATTACGAGCATT+AGG | + | chr6.4:11298142-11298161 | None:intergenic | 35.0% | |
| AACTGAACAACAAAAGGCTA+AGG | - | chr6.4:11297991-11298010 | MS.gene67990:CDS | 35.0% | |
| ACTTCTCTATTAACACTGTG+TGG | - | chr6.4:11298223-11298242 | MS.gene67990:intron | 35.0% | |
| ATTAACACTGTGTGGAGAAA+AGG | - | chr6.4:11298231-11298250 | MS.gene67990:intron | 35.0% | |
| ATTCATTACGAGCATTAGGA+AGG | + | chr6.4:11298138-11298157 | None:intergenic | 35.0% | |
| CTGGAAATAATTATCTGGCT+TGG | - | chr6.4:11297900-11297919 | MS.gene67990:CDS | 35.0% | |
| TTCCTAATGCTCGTAATGAA+TGG | - | chr6.4:11298137-11298156 | MS.gene67990:intron | 35.0% | |
| ! | AGATGGTCAATCTTTTCATC+AGG | + | chr6.4:11298892-11298911 | None:intergenic | 35.0% |
| ! | CCTCAAAATCAAGTTTTGCA+AGG | + | chr6.4:11297868-11297887 | None:intergenic | 35.0% |
| ! | CCTTGCAAAACTTGATTTTG+AGG | - | chr6.4:11297865-11297884 | MS.gene67990:CDS | 35.0% |
| ! | CTTTTGGATCTTCTATGGTA+AGG | + | chr6.4:11298066-11298085 | None:intergenic | 35.0% |
| !!! | ACCTTCTAAAACCCTTTTCA+CGG | + | chr6.4:11298330-11298349 | None:intergenic | 35.0% |
| AATTGTATCTCCATGCCCTT+CGG | + | chr6.4:11297954-11297973 | None:intergenic | 40.0% | |
| CAGCAACAATACCGTGAAAA+GGG | - | chr6.4:11298316-11298335 | MS.gene67990:intron | 40.0% | |
| CTTAATATCATGCCTGCTTG+TGG | - | chr6.4:11298357-11298376 | MS.gene67990:intron | 40.0% | |
| GATCACTATGGACAAAATCG+TGG | - | chr6.4:11298469-11298488 | MS.gene67990:intron | 40.0% | |
| GTAGCAACACATGATCACTA+TGG | - | chr6.4:11298457-11298476 | MS.gene67990:intron | 40.0% | |
| GTTGTGGAAGAAAAGGTCAT+TGG | - | chr6.4:11298704-11298723 | MS.gene67990:CDS | 40.0% | |
| TGCTATCGTTGTGGAAGAAA+AGG | - | chr6.4:11298697-11298716 | MS.gene67990:CDS | 40.0% | |
| ! | GAAGGATGACAGTTTTCTGA+TGG | + | chr6.4:11298120-11298139 | None:intergenic | 40.0% |
| ! | GTGTTGCTACATTCACTTCT+GGG | + | chr6.4:11298447-11298466 | None:intergenic | 40.0% |
| ! | TATTTTGTTCTGCCACAAGC+AGG | + | chr6.4:11298372-11298391 | None:intergenic | 40.0% |
| ! | TGTGTTGCTACATTCACTTC+TGG | + | chr6.4:11298448-11298467 | None:intergenic | 40.0% |
| !! | GAGAGTATTTGCTATCGTTG+TGG | - | chr6.4:11298688-11298707 | MS.gene67990:CDS | 40.0% |
| !!! | ACCGTGAAAAGGGTTTTAGA+AGG | - | chr6.4:11298326-11298345 | MS.gene67990:intron | 40.0% |
| AGAAATCAGCAGCGTCTAAG+TGG | + | chr6.4:11298861-11298880 | None:intergenic | 45.0% | |
| CATTTAAATGCCGAAGGGCA+TGG | - | chr6.4:11297941-11297960 | MS.gene67990:CDS | 45.0% | |
| GAAATCAGCAGCGTCTAAGT+GGG | + | chr6.4:11298860-11298879 | None:intergenic | 45.0% | |
| GCAGCAACAATACCGTGAAA+AGG | - | chr6.4:11298315-11298334 | MS.gene67990:intron | 45.0% | |
| TATGGACAAAATCGTGGTCG+TGG | - | chr6.4:11298475-11298494 | MS.gene67990:intron | 45.0% | |
| TTTCACGGTATTGTTGCTGC+AGG | + | chr6.4:11298315-11298334 | None:intergenic | 45.0% | |
| ! | GCTACATTCACTTCTGGGAA+TGG | + | chr6.4:11298442-11298461 | None:intergenic | 45.0% |
| ! | GTGGTGACGAAGGAATATCA+TGG | + | chr6.4:11298017-11298036 | None:intergenic | 45.0% |
| !!! | AAGATGTTTTGGCGTACGAC+AGG | + | chr6.4:11298737-11298756 | None:intergenic | 45.0% |
| ACGTGGTCGTGGTTATGGAA+GGG | - | chr6.4:11298528-11298547 | MS.gene67990:intron | 50.0% | |
| CCTTCGTCACCACCTTCATA+AGG | - | chr6.4:11298024-11298043 | MS.gene67990:CDS | 50.0% | |
| ! | CCTTATGAAGGTGGTGACGA+AGG | + | chr6.4:11298027-11298046 | None:intergenic | 50.0% |
| ! | TATGGAAGGGGACGTGGTTT+TGG | - | chr6.4:11298541-11298560 | MS.gene67990:intron | 50.0% |
| CGTGGTCGTGGTTATGGAAG+GGG | - | chr6.4:11298529-11298548 | MS.gene67990:intron | 55.0% | |
| GACGTGGTCGTGGTTATGGA+AGG | - | chr6.4:11298527-11298546 | MS.gene67990:intron | 55.0% | |
| TACGTCCACGACCATGACCA+CGG | + | chr6.4:11298501-11298520 | None:intergenic | 55.0% | |
| ! | GGGAATGGAGCACTACCAGT+GGG | + | chr6.4:11298427-11298446 | None:intergenic | 55.0% |
| ! | TGGGAATGGAGCACTACCAG+TGG | + | chr6.4:11298428-11298447 | None:intergenic | 55.0% |
| CAAAATCGTGGTCGTGGCCG+TGG | - | chr6.4:11298481-11298500 | MS.gene67990:intron | 60.0% | |
| CATGGTCGTGGACGTAGACG+TGG | - | chr6.4:11298505-11298524 | MS.gene67990:intron | 60.0% | |
| CGTGGACGTGGTCGTGGTTA+TGG | - | chr6.4:11298523-11298542 | MS.gene67990:intron | 60.0% | |
| CGTGGTTATGGAAGGGGACG+TGG | - | chr6.4:11298535-11298554 | MS.gene67990:intron | 60.0% | |
| ! | AATCACTCGGCTCGTCCCAC+TGG | - | chr6.4:11298409-11298428 | MS.gene67990:intron | 60.0% |
| CGTAGACGTGGACGTGGTCG+TGG | - | chr6.4:11298517-11298536 | MS.gene67990:intron | 65.0% | |
| CGTGGACGTAGACGTGGACG+TGG | - | chr6.4:11298511-11298530 | MS.gene67990:intron | 65.0% | |
| CGTGGCCGTGGTCATGGTCG+TGG | - | chr6.4:11298493-11298512 | MS.gene67990:intron | 70.0% | |
| CGTGGTCGTGGCCGTGGTCA+TGG | - | chr6.4:11298487-11298506 | MS.gene67990:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 11297857 | 11298939 | 11297857 | ID=MS.gene67990 |
| chr6.4 | mRNA | 11297857 | 11298939 | 11297857 | ID=MS.gene67990.t1;Parent=MS.gene67990 |
| chr6.4 | exon | 11298638 | 11298939 | 11298638 | ID=MS.gene67990.t1.exon1;Parent=MS.gene67990.t1 |
| chr6.4 | CDS | 11298638 | 11298939 | 11298638 | ID=cds.MS.gene67990.t1;Parent=MS.gene67990.t1 |
| chr6.4 | exon | 11297857 | 11298118 | 11297857 | ID=MS.gene67990.t1.exon2;Parent=MS.gene67990.t1 |
| chr6.4 | CDS | 11297857 | 11298118 | 11297857 | ID=cds.MS.gene67990.t1;Parent=MS.gene67990.t1 |
| Gene Sequence |
| Protein sequence |
