AlfalfaGEDB | MS.gene050221

M. sativa cultivar XinJiangDaYe / MS.gene050221

KEGG: K14431
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050221.t1	XP_003591665.2	71.3	87	22	1	4	87	119	205	2.20E-23	118.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050221.t1	I3S1H9	71.3	87	22	1	4	87	119	205	1.6e-23	118.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene050221	0.803694	3.09E-49	-1.69E-46
MS.gene049711	MS.gene050221	0.813748	2.19E-51	-1.69E-46
MS.gene050218	MS.gene050221	0.864682	1.02E-64	-1.69E-46
MS.gene050219	MS.gene050221	0.855014	8.38E-62	-1.69E-46
MS.gene050221	MS.gene050657	0.802442	5.60E-49	-1.69E-46
MS.gene050221	MS.gene051710	0.808182	3.51E-50	-1.69E-46
MS.gene050221	MS.gene052037	0.817977	2.49E-52	-1.69E-46
MS.gene050221	MS.gene05422	0.806371	8.50E-50	-1.69E-46
MS.gene050221	MS.gene054651	0.802594	5.21E-49	-1.69E-46
MS.gene050221	MS.gene058515	0.807708	4.43E-50	-1.69E-46
MS.gene050221	MS.gene059272	0.824154	9.41E-54	-1.69E-46
MS.gene050221	MS.gene059276	0.831115	2.00E-55	-1.69E-46
MS.gene050221	MS.gene064025	0.819375	1.20E-52	-1.69E-46
MS.gene050221	MS.gene066335	0.82984	4.11E-55	-1.69E-46
MS.gene050221	MS.gene066834	0.805454	1.32E-49	-1.69E-46
MS.gene050221	MS.gene067952	0.806073	9.82E-50	-1.69E-46
MS.gene050221	MS.gene07876	0.80296	4.38E-49	-1.69E-46
MS.gene050221	MS.gene21599	0.805239	1.47E-49	-1.69E-46
MS.gene050221	MS.gene22267	0.803477	3.42E-49	-1.69E-46
MS.gene050221	MS.gene22707	0.816126	6.49E-52	-1.69E-46
MS.gene050221	MS.gene24229	0.808315	3.29E-50	-1.69E-46
MS.gene050221	MS.gene25978	0.812153	4.89E-51	-1.69E-46
MS.gene050221	MS.gene26380	0.801535	8.60E-49	-1.69E-46
MS.gene050221	MS.gene26610	0.802706	4.94E-49	-1.69E-46
MS.gene050221	MS.gene26659	0.800339	1.51E-48	-1.69E-46
MS.gene050221	MS.gene27006	0.802043	6.76E-49	-1.69E-46
MS.gene050221	MS.gene27421	0.814285	1.66E-51	-1.69E-46
MS.gene050221	MS.gene28461	0.821754	3.41E-53	-1.69E-46
MS.gene050221	MS.gene29009	0.808335	3.26E-50	-1.69E-46
MS.gene050221	MS.gene31231	0.840369	9.06E-58	-1.69E-46
MS.gene050221	MS.gene31433	0.804869	1.76E-49	-1.69E-46
MS.gene050221	MS.gene34287	0.805588	1.24E-49	-1.69E-46
MS.gene050221	MS.gene34667	0.803313	3.70E-49	-1.69E-46
MS.gene050221	MS.gene35858	0.802446	5.59E-49	-1.69E-46
MS.gene050221	MS.gene36151	0.803269	3.78E-49	-1.69E-46
MS.gene050221	MS.gene37917	0.806391	8.41E-50	-1.69E-46
MS.gene050221	MS.gene38011	0.818671	1.74E-52	-1.69E-46
MS.gene050221	MS.gene41113	0.811071	8.41E-51	-1.69E-46
MS.gene050221	MS.gene48297	0.802394	5.73E-49	-1.69E-46
MS.gene050221	MS.gene48919	0.828224	1.01E-54	-1.69E-46
MS.gene050221	MS.gene50875	0.801631	8.22E-49	-1.69E-46
MS.gene050221	MS.gene51818	0.802224	6.21E-49	-1.69E-46
MS.gene050221	MS.gene58547	0.812111	5.00E-51	-1.69E-46
MS.gene050221	MS.gene58802	0.812904	3.35E-51	-1.69E-46
MS.gene050221	MS.gene62491	0.803821	2.90E-49	-1.69E-46
MS.gene050221	MS.gene64567	0.808339	3.25E-50	-1.69E-46
MS.gene050221	MS.gene66682	0.820903	5.36E-53	-1.69E-46
MS.gene050221	MS.gene66700	0.833852	4.20E-56	-1.69E-46
MS.gene050221	MS.gene67990	0.806732	7.13E-50	-1.69E-46
MS.gene050221	MS.gene67991	0.803187	3.93E-49	-1.69E-46
MS.gene050221	MS.gene68388	0.800382	1.48E-48	-1.69E-46
MS.gene050221	MS.gene71430	0.804862	1.76E-49	-1.69E-46
MS.gene050221	MS.gene71440	0.806582	7.67E-50	-1.69E-46
MS.gene050221	MS.gene77149	0.803183	3.94E-49	-1.69E-46
MS.gene050221	MS.gene80732	0.801511	8.70E-49	-1.69E-46
MS.gene050221	MS.gene80853	0.808672	2.76E-50	-1.69E-46
MS.gene050221	MS.gene82962	0.803631	3.18E-49	-1.69E-46
MS.gene050221	MS.gene85692	0.814235	1.71E-51	-1.69E-46
MS.gene050221	MS.gene85694	0.805896	1.07E-49	-1.69E-46
MS.gene050221	MS.gene85698	0.832971	6.97E-56	-1.69E-46
MS.gene050221	MS.gene88171	0.83239	9.71E-56	-1.69E-46
MS.gene050221	MS.gene90823	0.805021	1.63E-49	-1.69E-46
MS.gene050221	MS.gene98611	0.827864	1.24E-54	-1.69E-46
MS.gene050221	MS.gene98612	0.811921	5.50E-51	-1.69E-46
MS.gene050221	MS.gene99746	0.805339	1.40E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050221.t1	MTR_1g090370	71.264	87	22	1	4	87	107	193	7.26e-38	127

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 13 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTTATGATATTTATAATCT+TGG	0.237785	1.2:+67661497	MS.gene050221:CDS
TCGTCCTTCTCAACTTCTTC+AGG	0.297180	1.2:+67661220	MS.gene050221:CDS
TTGCTTTATCAATGATTTGT+TGG	0.412400	1.2:-67661571	None:intergenic
TTACCTCTTTGAAGAAGCTT+AGG	0.413182	1.2:-67661640	None:intergenic
ATTGATAAAGCAACATTAGC+AGG	0.485264	1.2:+67661581	MS.gene050221:CDS
TTGAAGAAGCTTAGGTGTTG+AGG	0.516308	1.2:-67661632	None:intergenic
CGCAATCTATGGTGGATTGG+TGG	0.541391	1.2:+67661194	None:intergenic
GTGCACAAATGTTTCAGTTG+AGG	0.592785	1.2:-67661465	None:intergenic
ACACCTAAGCTTCTTCAAAG+AGG	0.598796	1.2:+67661637	MS.gene050221:CDS
GAACGCAATCTATGGTGGAT+TGG	0.599907	1.2:+67661191	None:intergenic
GCTGAATATGCTCTTGCACA+AGG	0.606518	1.2:+67661536	MS.gene050221:CDS
GAAAATTGATGCAAGAATTG+CGG	0.620569	1.2:-67661789	None:intergenic
ATTACCTGAAGAAGTTGAGA+AGG	0.659326	1.2:-67661224	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTTTATGATATTTATAATCT+TGG	+	chr1.2:67661497-67661516	MS.gene050221:CDS	15.0%
!!	AAGTACTATGTGATTTAATA+TGG	+	chr1.2:67661423-67661442	MS.gene050221:intron	20.0%
!!	GATTTAATATGGATATACAT+AGG	+	chr1.2:67661434-67661453	MS.gene050221:intron	20.0%
!!	GTAAGAAACATAATTCAAAT+TGG	-	chr1.2:67661403-67661422	None:intergenic	20.0%
!	ATCATATAGAAAAACTGACT+TGG	+	chr1.2:67661736-67661755	MS.gene050221:intron	25.0%
!	GTAGGGTTAAACTTAAAAAT+AGG	-	chr1.2:67661668-67661687	None:intergenic	25.0%
!!	TTGCTTTATCAATGATTTGT+TGG	-	chr1.2:67661574-67661593	None:intergenic	25.0%
	AACTAATTCTACCCCTAATT+AGG	-	chr1.2:67661365-67661384	None:intergenic	30.0%
	ATTGATAAAGCAACATTAGC+AGG	+	chr1.2:67661581-67661600	MS.gene050221:CDS	30.0%
	ATTGATGTGATGTCCTAATT+AGG	+	chr1.2:67661349-67661368	MS.gene050221:intron	30.0%
	GAAAATTGATGCAAGAATTG+CGG	-	chr1.2:67661792-67661811	None:intergenic	30.0%
	TTGATGTGATGTCCTAATTA+GGG	+	chr1.2:67661350-67661369	MS.gene050221:intron	30.0%
	ACTTGGTTTCTTGTAACTAC+AGG	+	chr1.2:67661753-67661772	MS.gene050221:intron	35.0%
	ATTACCTGAAGAAGTTGAGA+AGG	-	chr1.2:67661227-67661246	None:intergenic	35.0%
	TGATGTGATGTCCTAATTAG+GGG	+	chr1.2:67661351-67661370	MS.gene050221:intron	35.0%
!	TTACCTCTTTGAAGAAGCTT+AGG	-	chr1.2:67661643-67661662	None:intergenic	35.0%
	ACACCTAAGCTTCTTCAAAG+AGG	+	chr1.2:67661637-67661656	MS.gene050221:CDS	40.0%
	GTGCACAAATGTTTCAGTTG+AGG	-	chr1.2:67661468-67661487	None:intergenic	40.0%
!	TTGAAGAAGCTTAGGTGTTG+AGG	-	chr1.2:67661635-67661654	None:intergenic	40.0%
	ACCCTACACACTCTCTAACA+CGG	+	chr1.2:67661681-67661700	MS.gene050221:intron	45.0%
	GCTGAATATGCTCTTGCACA+AGG	+	chr1.2:67661536-67661555	MS.gene050221:CDS	45.0%
	TCGTCCTTCTCAACTTCTTC+AGG	+	chr1.2:67661220-67661239	MS.gene050221:CDS	45.0%
!	AGCCGTGTTAGAGAGTGTGT+AGG	-	chr1.2:67661686-67661705	None:intergenic	50.0%
!	GCCGTGTTAGAGAGTGTGTA+GGG	-	chr1.2:67661685-67661704	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	67661202	67661849	67661202	ID=MS.gene050221
chr1.2	mRNA	67661202	67661849	67661202	ID=MS.gene050221.t1;Parent=MS.gene050221
chr1.2	exon	67661202	67661241	67661202	ID=MS.gene050221.t1.exon1;Parent=MS.gene050221.t1
chr1.2	CDS	67661202	67661241	67661202	ID=cds.MS.gene050221.t1;Parent=MS.gene050221.t1
chr1.2	exon	67661456	67661658	67661456	ID=MS.gene050221.t1.exon2;Parent=MS.gene050221.t1
chr1.2	CDS	67661456	67661658	67661456	ID=cds.MS.gene050221.t1;Parent=MS.gene050221.t1
chr1.2	exon	67661775	67661849	67661775	ID=MS.gene050221.t1.exon3;Parent=MS.gene050221.t1
chr1.2	CDS	67661775	67661849	67661775	ID=cds.MS.gene050221.t1;Parent=MS.gene050221.t1

Gene Sequence

>MS.gene050221

ATGGTGGATTGGTGGCTTTCGTCCTTCTCAACTTCTTCAGGTAATGCATGTTGATATTTTGTAAATATGAGTCAGACTCAATTTCAACACTATATATTGTCACTTACGCATTCATTTTGTTCTTTATATTTTTTTAGTGCTAAAAAGATTGATGTGATGTCCTAATTAGGGGTAGAATTAGTTAGAAACATATACACACCAATTTGAATTATGTTTCTTACAAGTACTATGTGATTTAATATGGATATACATAGGTTATTCTGCCTCAACTGAAACATTTGTGCACTCAACAACACTTTATGATATTTATAATCTTGGACAATCATGTCAACAAGCTGAATATGCTCTTGCACAAGGCATGATAGAACTCCAACAAATCATTGATAAAGCAACATTAGCAGGTGACAAAGAGTTTCAGCAGACGTGTGTACCTCAACACCTAAGCTTCTTCAAAGAGGTAATACCTATTTTTAAGTTTAACCCTACACACTCTCTAACACGGCTCGATTATAAGTTGAAATTCATGCGAGTCTCATCATATAGAAAAACTGACTTGGTTTCTTGTAACTACAGGCTGATAATCTTCGCCGCAATTCTTGCATCAATTTTCTCGTCTCTTTACAATTTCTCAGCAAGCTGAGCTCGTAG

Protein sequence

>MS.gene050221.t1

MVDWWLSSFSTSSGYSASTETFVHSTTLYDIYNLGQSCQQAEYALAQGMIELQQIIDKATLAGDKEFQQTCVPQHLSFFKEADNLRRNSCINFLVSLQFLSKLSS