AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68005

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68005.t1 MTR_6g018400 58.311 379 97 6 1 329 1 368 1.58e-133 384
MS.gene68005.t1 MTR_6g027100 53.077 390 100 9 1 325 1 372 7.60e-118 347
MS.gene68005.t1 MTR_6g027040 52.308 390 103 9 1 325 1 372 4.58e-117 343
MS.gene68005.t1 MTR_6g018360 51.145 393 115 9 1 329 1 380 3.19e-109 324
MS.gene68005.t1 MTR_6g018390 54.688 384 100 11 1 329 1 365 1.96e-108 320
MS.gene68005.t1 MTR_6g018315 49.479 384 106 11 16 327 1 368 8.59e-99 297
MS.gene68005.t1 MTR_6g018370 57.934 271 81 5 4 262 3 252 4.88e-90 270
MS.gene68005.t1 MTR_2g047180 44.133 392 126 11 3 329 2 365 1.98e-85 262
MS.gene68005.t1 MTR_3g029650 45.161 372 149 8 1 329 1 360 8.86e-84 268
MS.gene68005.t1 MTR_3g029650 40.609 394 148 10 1 324 368 745 3.48e-72 238
MS.gene68005.t1 MTR_1g007260 42.377 387 149 9 1 329 18 388 3.05e-83 257
MS.gene68005.t1 MTR_1g026850 45.646 379 129 12 4 327 5 361 1.02e-82 255
MS.gene68005.t1 MTR_3g030080 42.172 396 133 11 2 329 4 371 6.46e-78 243
MS.gene68005.t1 MTR_3g029920 40.759 395 151 14 1 329 1 378 1.12e-73 232
MS.gene68005.t1 MTR_3g030380 42.520 381 142 13 1 329 1 356 8.77e-73 229
MS.gene68005.t1 MTR_3g030480 41.444 374 144 12 1 324 1 349 5.96e-70 222
MS.gene68005.t1 MTR_3g030440 41.287 373 144 12 2 324 3 350 1.80e-69 221
MS.gene68005.t1 MTR_3g029640 40.992 383 149 14 3 329 2 363 2.37e-69 221
MS.gene68005.t1 MTR_3g030360 39.529 382 140 10 1 326 1 347 4.62e-69 219
MS.gene68005.t1 MTR_1g016570 40.160 376 135 11 1 324 17 354 5.88e-66 212
MS.gene68005.t1 MTR_3g029840 39.831 354 129 7 1 292 1 332 1.08e-64 208
MS.gene68005.t1 MTR_3g092000 39.659 411 153 14 1 329 1 398 4.49e-64 208
MS.gene68005.t1 MTR_3g029950 39.231 390 139 11 1 322 1 360 3.12e-63 205
MS.gene68005.t1 MTR_3g029800 37.113 388 149 13 3 329 2 355 1.20e-61 201
MS.gene68005.t1 MTR_3g029620 37.532 389 162 10 4 329 2 372 3.01e-61 200
MS.gene68005.t1 MTR_3g030560 35.825 388 169 9 4 329 3 372 1.35e-59 196
MS.gene68005.t1 MTR_1g049170 36.272 397 166 10 3 329 4 383 1.68e-56 188
MS.gene68005.t1 MTR_3g030355 44.030 268 99 10 80 324 150 389 3.07e-54 182
MS.gene68005.t1 MTR_3g030355 55.000 80 30 2 2 75 3 82 2.82e-14 73.6
MS.gene68005.t1 MTR_5g042230 57.979 188 57 6 143 319 13 189 8.64e-54 175
MS.gene68005.t1 MTR_3g058460 36.176 387 167 10 1 329 11 375 2.61e-53 179
MS.gene68005.t1 MTR_3g029870 38.095 357 130 9 1 314 1 309 1.82e-52 176
MS.gene68005.t1 MTR_1g007220 50.242 207 50 5 127 322 3 167 3.34e-52 170
MS.gene68005.t1 MTR_3g091990 43.525 278 110 8 80 329 127 385 1.19e-51 176
MS.gene68005.t1 MTR_3g030050 31.702 429 171 11 2 329 13 420 2.31e-50 173
MS.gene68005.t1 MTR_3g029750 39.850 266 134 7 74 324 127 381 1.23e-49 170
MS.gene68005.t1 MTR_3g029750 59.649 57 23 0 1 57 1 57 4.14e-14 72.8
MS.gene68005.t1 MTR_3g029670 39.189 296 149 8 38 324 108 381 8.17e-49 168
MS.gene68005.t1 MTR_3g029670 52.703 74 30 1 1 74 1 69 6.56e-15 75.1
MS.gene68005.t1 MTR_3g029820 39.831 354 160 16 1 329 1 326 1.06e-48 166
MS.gene68005.t1 MTR_3g047980 37.197 371 146 13 5 329 30 359 1.56e-48 167
MS.gene68005.t1 MTR_1g016540 52.459 183 72 5 157 329 1 178 3.12e-48 160
MS.gene68005.t1 MTR_1g007250 57.143 175 51 5 157 322 1 160 1.50e-47 158
MS.gene68005.t1 MTR_3g029900 42.400 250 112 5 80 322 227 451 1.42e-45 162
MS.gene68005.t1 MTR_7g005990 38.760 258 118 9 80 329 131 356 1.41e-40 145
MS.gene68005.t1 MTR_3g037710 37.395 238 111 5 114 329 41 262 1.63e-40 143
MS.gene68005.t1 MTR_8g032080 57.143 98 42 0 79 176 2 99 7.41e-30 116
MS.gene68005.t1 MTR_5g078450 52.414 145 27 4 191 326 2 113 5.46e-29 108
MS.gene68005.t1 MTR_4g022880 42.953 149 67 3 198 329 24 171 2.25e-25 100
MS.gene68005.t1 MTR_1g007200 56.566 99 30 3 223 314 1 93 4.86e-20 84.7
MS.gene68005.t1 MTR_6g018420 97.297 37 1 0 1 37 1 37 1.32e-19 82.4
MS.gene68005.t1 MTR_2g041510 25.707 389 202 12 4 327 25 391 3.39e-18 85.1
MS.gene68005.t1 MTR_2g041510 25.707 389 202 12 4 327 5 371 3.77e-18 84.7
MS.gene68005.t1 MTR_6g009620 61.017 59 23 0 79 137 68 126 2.57e-16 74.7
MS.gene68005.t1 MTR_1g007230 60.000 60 23 1 2 60 5 64 9.80e-16 72.0
MS.gene68005.t1 MTR_7g012990 42.017 119 45 2 110 228 55 149 1.59e-15 73.6
MS.gene68005.t1 MTR_8g035995 40.385 104 41 2 166 260 1 92 7.49e-15 71.2
MS.gene68005.t1 MTR_5g079830 25.926 324 165 11 4 272 5 308 3.06e-14 73.2
MS.gene68005.t1 MTR_6g075925 64.286 42 15 0 1 42 1 42 6.88e-12 61.2
MS.gene68005.t1 MTR_8g074610 31.098 164 80 6 143 285 210 361 5.50e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68005.t1 AT2G17030 28.000 400 197 9 4 326 2 387 2.92e-34 130
MS.gene68005.t1 AT2G17036 29.008 393 196 15 4 326 2 381 6.02e-28 112
MS.gene68005.t1 AT2G17036 29.008 393 196 15 4 326 1 380 7.34e-28 112
MS.gene68005.t1 AT5G60060 30.000 340 165 11 6 285 11 337 2.11e-24 102

Find 85 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTACATATTCCCTAAAA+AGG 0.197211 6.1:-4328173 MS.gene68005:CDS
CTGAATATTCCAACTTGTTT+TGG 0.229967 6.1:-4327559 MS.gene68005:CDS
CAAATTATCTGTCCTCCATT+TGG 0.288391 6.1:-4328254 MS.gene68005:CDS
CATAAGGGTGCCCTTTAAAA+AGG 0.297560 6.1:+4328011 None:intergenic
CCAAAGGTTCAGCCACTAAA+TGG 0.299723 6.1:+4327939 None:intergenic
GGGTGAAGTTAACGAGTTTA+GGG 0.316381 6.1:-4327766 MS.gene68005:CDS
CCTTATGCGGTTGATGAAAA+TGG 0.316777 6.1:-4327995 MS.gene68005:CDS
CAAAACAAGTTGGAATATTC+AGG 0.336277 6.1:+4327560 None:intergenic
TGGGTGAAGTTAACGAGTTT+AGG 0.340496 6.1:-4327767 MS.gene68005:CDS
GAACCGTGCGAGCTATGTTT+TGG 0.351429 6.1:-4327621 MS.gene68005:CDS
CCATTTAGTGGCTGAACCTT+TGG 0.363928 6.1:-4327939 MS.gene68005:CDS
AAATTATCTGTCCTCCATTT+GGG 0.378956 6.1:-4328253 MS.gene68005:CDS
ACGTTTATGTGCATGATGTA+AGG 0.383020 6.1:-4327826 MS.gene68005:CDS
TAATTTGAAGAGTGGGAACT+TGG 0.393000 6.1:+4328519 None:intergenic
CATCCATGGCGGCGGTTGAC+TGG 0.409781 6.1:-4328675 None:intergenic
CGAAATGTGGCAATGAGAAC+TGG 0.410594 6.1:-4328075 MS.gene68005:CDS
ATTAGTGATGATATCTTAAG+AGG 0.424783 6.1:-4327659 MS.gene68005:CDS
CCATACACCATTGACATGTT+GGG 0.425510 6.1:+4328043 None:intergenic
TGGAGGGTTATTGCTGTTGA+TGG 0.434908 6.1:+4328465 None:intergenic
AGACTCAAATGTCCATTTAG+TGG 0.446433 6.1:-4327951 MS.gene68005:CDS
TCCATACACCATTGACATGT+TGG 0.451101 6.1:+4328042 None:intergenic
CATTTCGAAAGTAACGGAAA+TGG 0.457568 6.1:+4328091 None:intergenic
AATGATCACACGATTCCCTT+TGG 0.457670 6.1:+4327679 None:intergenic
ATTTCGAAAGTAACGGAAAT+GGG 0.457898 6.1:+4328092 None:intergenic
CCCAACATGTCAATGGTGTA+TGG 0.460232 6.1:-4328043 MS.gene68005:CDS
AGGAATGGTAATTTGAAGAG+TGG 0.461097 6.1:+4328511 None:intergenic
TTTGAAGAGTGGGAACTTGG+AGG 0.462532 6.1:+4328522 None:intergenic
TCGGAAGAATAAATTATTAG+TGG 0.464956 6.1:-4327876 MS.gene68005:CDS
TTTAAAGCGGCAACAATGAG+AGG 0.475144 6.1:+4328121 None:intergenic
CCATGTAGAGGGTGAACCTT+CGG 0.477848 6.1:-4327906 MS.gene68005:CDS
TTGAAGAGTGGGAACTTGGA+GGG 0.478746 6.1:+4328523 None:intergenic
GTGGCTGAACCTTTGGTTGG+TGG 0.479791 6.1:-4327932 MS.gene68005:CDS
TTTGATGCCGCAGTTGCAAA+TGG 0.486884 6.1:+4328219 None:intergenic
TGGAAATGATGCTTCCCAAA+TGG 0.488597 6.1:+4328239 None:intergenic
AATGGTAAGACAGTTATTGT+TGG 0.490052 6.1:-4327977 MS.gene68005:CDS
GTTGGAATATTCAGGAGAAT+CGG 0.493339 6.1:+4327568 None:intergenic
ATCATTTCCATTTGCAACTG+CGG 0.497280 6.1:-4328226 MS.gene68005:CDS
CAGATTTGTCTGTTGCCAAA+GGG 0.499116 6.1:-4327694 MS.gene68005:CDS
CCGAAGGTTCACCCTCTACA+TGG 0.509419 6.1:+4327906 None:intergenic
GAGGGTGAACCTTCGGTTGG+TGG 0.524060 6.1:-4327899 MS.gene68005:CDS
TCAGATTTGTCTGTTGCCAA+AGG 0.532428 6.1:-4327695 MS.gene68005:CDS
TTTGTTCAAGCTTAATGAGA+AGG 0.533518 6.1:-4327798 MS.gene68005:CDS
TTAATGAGAAGGAGAAGAAG+TGG 0.534717 6.1:-4327787 MS.gene68005:CDS
CGATGGAGATTTGTTGTTAG+CGG 0.535070 6.1:-4327849 MS.gene68005:CDS
TTAGTGGCTGAACCTTTGGT+TGG 0.535767 6.1:-4327935 MS.gene68005:CDS
TTTCCGTTACTTTCGAAATG+TGG 0.536550 6.1:-4328088 MS.gene68005:CDS
GTTGTTGCTGCCACGTGCCA+TGG 0.539751 6.1:-4328151 MS.gene68005:CDS
GGTGATCCCCAACATGTCAA+TGG 0.543625 6.1:-4328050 MS.gene68005:CDS
GTTGGTGGTGTCCATGTAGA+GGG 0.549184 6.1:-4327917 MS.gene68005:CDS
TTGCCACATTTCGAAAGTAA+CGG 0.550781 6.1:+4328085 None:intergenic
TAATGAGAAGGAGAAGAAGT+GGG 0.560700 6.1:-4327786 MS.gene68005:CDS
CCATGTCGTTGGCGTCAGAA+AGG 0.562079 6.1:+4328491 None:intergenic
GGAATGGTAATTTGAAGAGT+GGG 0.563430 6.1:+4328512 None:intergenic
ACTCAGATCCGCCTAGGACG+TGG 0.563518 6.1:+4327536 None:intergenic
GGTTGGTGGTGTCCATGTAG+AGG 0.566033 6.1:-4327918 MS.gene68005:CDS
ATCAGATTGAGAAGCTCCGT+TGG 0.566429 6.1:+4328643 None:intergenic
CCTTTCTGACGCCAACGACA+TGG 0.568942 6.1:-4328491 MS.gene68005:CDS
AGGACGTGGCCAAAACAAGT+TGG 0.576346 6.1:+4327550 None:intergenic
CGTTTATGTGCATGATGTAA+GGG 0.586949 6.1:-4327825 MS.gene68005:CDS
GTTGATGGTATCCATGTCGT+TGG 0.587062 6.1:+4328480 None:intergenic
TCCGATCAACTTGTTCAACA+TGG 0.587534 6.1:-4328582 MS.gene68005:CDS
TTCTTCCGACCACCAACCGA+AGG 0.587579 6.1:+4327890 None:intergenic
AGACCAGTCAACCGCCGCCA+TGG 0.588730 6.1:+4328672 None:intergenic
GTGAACCTTCGGTTGGTGGT+CGG 0.592129 6.1:-4327895 MS.gene68005:CDS
GCTATAAGCATCCATGGCGG+CGG 0.594630 6.1:-4328683 None:intergenic
TGGAGAGGCGACAGAATGGA+GGG 0.599874 6.1:+4328449 None:intergenic
TTGGAGAGGCGACAGAATGG+AGG 0.604058 6.1:+4328448 None:intergenic
AAATTATTAGTGGAGAGCGA+TGG 0.609711 6.1:-4327866 MS.gene68005:CDS
GCCATGTTGAACAAGTTGAT+CGG 0.610376 6.1:+4328581 None:intergenic
TTAAAGCGGCAACAATGAGA+GGG 0.618743 6.1:+4328122 None:intergenic
GTAGAGGGTGAACCTTCGGT+TGG 0.622007 6.1:-4327902 MS.gene68005:CDS
CATACACCATTGACATGTTG+GGG 0.623025 6.1:+4328044 None:intergenic
AGTCCAAAACATAGCTCGCA+CGG 0.623098 6.1:+4327618 None:intergenic
TGACTGGTCTAAACTTCCAA+CGG 0.624352 6.1:-4328659 MS.gene68005:CDS
TAACGAACTCAGATCCGCCT+AGG 0.630137 6.1:+4327530 None:intergenic
AAATGATGCTTCCCAAATGG+AGG 0.645415 6.1:+4328242 None:intergenic
TATTCAGGAGAATCGGACAA+AGG 0.650496 6.1:+4327575 None:intergenic
ACATGGACACCACCAACCAA+AGG 0.653214 6.1:+4327923 None:intergenic
TAAGAGGATATTGTAGACGT+AGG 0.667353 6.1:-4327643 MS.gene68005:CDS
GGTGAAGTTAACGAGTTTAG+GGG 0.668574 6.1:-4327765 MS.gene68005:CDS
TCAGGAGAATCGGACAAAGG+CGG 0.677233 6.1:+4327578 None:intergenic
TCGTTGGCGTCAGAAAGGAA+TGG 0.697034 6.1:+4328496 None:intergenic
GAATATGTAGAAACAATCGT+CGG 0.699359 6.1:+4328183 None:intergenic
AAATGGGCGAGCATTTAAAG+CGG 0.718665 6.1:+4328108 None:intergenic
ATGTGGCAATGAGAACTGGA+AGG 0.732321 6.1:-4328071 MS.gene68005:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATTAGTGATGATATCTTAAG+AGG - chr6.1:4328549-4328568 MS.gene68005:CDS 25.0%
! TCGGAAGAATAAATTATTAG+TGG - chr6.1:4328332-4328351 MS.gene68005:intron 25.0%
! TTTCTACATATTCCCTAAAA+AGG - chr6.1:4328035-4328054 MS.gene68005:CDS 25.0%
!! GTAACATTTGAAGAAGTTTT+AGG + chr6.1:4327666-4327685 None:intergenic 25.0%
AAATTATCTGTCCTCCATTT+GGG - chr6.1:4327955-4327974 MS.gene68005:CDS 30.0%
AATGGTAAGACAGTTATTGT+TGG - chr6.1:4328231-4328250 MS.gene68005:CDS 30.0%
GAATATGTAGAAACAATCGT+CGG + chr6.1:4328028-4328047 None:intergenic 30.0%
TTTGTTCAAGCTTAATGAGA+AGG - chr6.1:4328410-4328429 MS.gene68005:intron 30.0%
! ATTTCGAAAGTAACGGAAAT+GGG + chr6.1:4328119-4328138 None:intergenic 30.0%
! CAAAACAAGTTGGAATATTC+AGG + chr6.1:4328651-4328670 None:intergenic 30.0%
! GAGACATATGCCTTTTTAAA+GGG - chr6.1:4328187-4328206 MS.gene68005:CDS 30.0%
!! CTGAATATTCCAACTTGTTT+TGG - chr6.1:4328649-4328668 MS.gene68005:CDS 30.0%
!!! CATTTGAAGAAGTTTTAGGA+CGG + chr6.1:4327662-4327681 None:intergenic 30.0%
AAATTATTAGTGGAGAGCGA+TGG - chr6.1:4328342-4328361 MS.gene68005:intron 35.0%
ACAAGACCTTTACAAGAAAG+AGG + chr6.1:4327922-4327941 None:intergenic 35.0%
ACGTTTATGTGCATGATGTA+AGG - chr6.1:4328382-4328401 MS.gene68005:intron 35.0%
AGACTCAAATGTCCATTTAG+TGG - chr6.1:4328257-4328276 MS.gene68005:CDS 35.0%
AGGAATGGTAATTTGAAGAG+TGG + chr6.1:4327700-4327719 None:intergenic 35.0%
ATCATTTCCATTTGCAACTG+CGG - chr6.1:4327982-4328001 MS.gene68005:CDS 35.0%
CAAATTATCTGTCCTCCATT+TGG - chr6.1:4327954-4327973 MS.gene68005:CDS 35.0%
CAAGACCTTTACAAGAAAGA+GGG + chr6.1:4327921-4327940 None:intergenic 35.0%
CGTTTATGTGCATGATGTAA+GGG - chr6.1:4328383-4328402 MS.gene68005:intron 35.0%
GGAATGGTAATTTGAAGAGT+GGG + chr6.1:4327699-4327718 None:intergenic 35.0%
GTTGGAATATTCAGGAGAAT+CGG + chr6.1:4328643-4328662 None:intergenic 35.0%
TAAGAGGATATTGTAGACGT+AGG - chr6.1:4328565-4328584 MS.gene68005:CDS 35.0%
TAATGAGAAGGAGAAGAAGT+GGG - chr6.1:4328422-4328441 MS.gene68005:intron 35.0%
TAATTTGAAGAGTGGGAACT+TGG + chr6.1:4327692-4327711 None:intergenic 35.0%
TATGACCCTCTTTCTTGTAA+AGG - chr6.1:4327913-4327932 MS.gene68005:CDS 35.0%
TTAATGAGAAGGAGAAGAAG+TGG - chr6.1:4328421-4328440 MS.gene68005:intron 35.0%
TTGCCACATTTCGAAAGTAA+CGG + chr6.1:4328126-4328145 None:intergenic 35.0%
! CATTTCGAAAGTAACGGAAA+TGG + chr6.1:4328120-4328139 None:intergenic 35.0%
! CATTTTCATCAACCGCATAA+GGG + chr6.1:4328215-4328234 None:intergenic 35.0%
! GATGAGGTAGAGATTTTGTT+TGG + chr6.1:4327782-4327801 None:intergenic 35.0%
! GGAGACATATGCCTTTTTAA+AGG - chr6.1:4328186-4328205 MS.gene68005:CDS 35.0%
! TTTCCGTTACTTTCGAAATG+TGG - chr6.1:4328120-4328139 MS.gene68005:CDS 35.0%
!! TATGTTTTGGACTTGGATCA+AGG - chr6.1:4328600-4328619 MS.gene68005:CDS 35.0%
!! TGTTCTTCTTGTTGTTGTTG+TGG + chr6.1:4327814-4327833 None:intergenic 35.0%
AAATGATGCTTCCCAAATGG+AGG + chr6.1:4327969-4327988 None:intergenic 40.0%
AAATGGGCGAGCATTTAAAG+CGG + chr6.1:4328103-4328122 None:intergenic 40.0%
AAGAAAGAGGGTCATAATCG+AGG + chr6.1:4327909-4327928 None:intergenic 40.0%
AATGATCACACGATTCCCTT+TGG + chr6.1:4328532-4328551 None:intergenic 40.0%
CAGATTTGTCTGTTGCCAAA+GGG - chr6.1:4328514-4328533 MS.gene68005:CDS 40.0%
CATACACCATTGACATGTTG+GGG + chr6.1:4328167-4328186 None:intergenic 40.0%
CCATACACCATTGACATGTT+GGG + chr6.1:4328168-4328187 None:intergenic 40.0%
GGGTGAAGTTAACGAGTTTA+GGG - chr6.1:4328442-4328461 MS.gene68005:intron 40.0%
GGTGAAGTTAACGAGTTTAG+GGG - chr6.1:4328443-4328462 MS.gene68005:intron 40.0%
TATTCAGGAGAATCGGACAA+AGG + chr6.1:4328636-4328655 None:intergenic 40.0%
TCAGATTTGTCTGTTGCCAA+AGG - chr6.1:4328513-4328532 MS.gene68005:CDS 40.0%
TCCATACACCATTGACATGT+TGG + chr6.1:4328169-4328188 None:intergenic 40.0%
TGGAAATGATGCTTCCCAAA+TGG + chr6.1:4327972-4327991 None:intergenic 40.0%
TGGGTGAAGTTAACGAGTTT+AGG - chr6.1:4328441-4328460 MS.gene68005:intron 40.0%
TTAAAGCGGCAACAATGAGA+GGG + chr6.1:4328089-4328108 None:intergenic 40.0%
! CCATTTTCATCAACCGCATA+AGG + chr6.1:4328216-4328235 None:intergenic 40.0%
! TCCGATCAACTTGTTCAACA+TGG - chr6.1:4327626-4327645 MS.gene68005:CDS 40.0%
! TGGCAGCAACAACCTTTTTA+GGG + chr6.1:4328050-4328069 None:intergenic 40.0%
! TTTAAAGCGGCAACAATGAG+AGG + chr6.1:4328090-4328109 None:intergenic 40.0%
!! CATAAGGGTGCCCTTTAAAA+AGG + chr6.1:4328200-4328219 None:intergenic 40.0%
!! CCTTATGCGGTTGATGAAAA+TGG - chr6.1:4328213-4328232 MS.gene68005:CDS 40.0%
!! CGATGGAGATTTGTTGTTAG+CGG - chr6.1:4328359-4328378 MS.gene68005:intron 40.0%
!! GCCATGTTGAACAAGTTGAT+CGG + chr6.1:4327630-4327649 None:intergenic 40.0%
!! GGTAGAGATTTTGTTTGGAG+AGG + chr6.1:4327777-4327796 None:intergenic 40.0%
!! TCTTCTTGTTGTTGTTGTGG+TGG + chr6.1:4327811-4327830 None:intergenic 40.0%
!! TGACTGGTCTAAACTTCCAA+CGG - chr6.1:4327549-4327568 MS.gene68005:CDS 40.0%
!!! ACAATCGTCGGCTTCATTTT+TGG + chr6.1:4328016-4328035 None:intergenic 40.0%
!!! AGGGGATAGAGTTTTGTTCT+TGG - chr6.1:4328461-4328480 MS.gene68005:CDS 40.0%
!!! CAATCGTCGGCTTCATTTTT+GGG + chr6.1:4328015-4328034 None:intergenic 40.0%
!!! CAATGAGAGGGTTTTTTCCA+TGG + chr6.1:4328077-4328096 None:intergenic 40.0%
!!! GGGATAGAGTTTTGTTCTTG+GGG - chr6.1:4328463-4328482 MS.gene68005:CDS 40.0%
!!! GGGGATAGAGTTTTGTTCTT+GGG - chr6.1:4328462-4328481 MS.gene68005:CDS 40.0%
!!! TTGAAGAAGTTTTAGGACGG+AGG + chr6.1:4327659-4327678 None:intergenic 40.0%
!!! TTTTAAAGGGCACCCTTATG+CGG - chr6.1:4328200-4328219 MS.gene68005:CDS 40.0%
AACAAACCCTACTTTGCCCT+CGG - chr6.1:4327830-4327849 MS.gene68005:CDS 45.0%
AGTCCAAAACATAGCTCGCA+CGG + chr6.1:4328593-4328612 None:intergenic 45.0%
ATCAGATTGAGAAGCTCCGT+TGG + chr6.1:4327568-4327587 None:intergenic 45.0%
ATGTGGCAATGAGAACTGGA+AGG - chr6.1:4328137-4328156 MS.gene68005:CDS 45.0%
CCAAAGGTTCAGCCACTAAA+TGG + chr6.1:4328272-4328291 None:intergenic 45.0%
CCATTTAGTGGCTGAACCTT+TGG - chr6.1:4328269-4328288 MS.gene68005:CDS 45.0%
CCCAACATGTCAATGGTGTA+TGG - chr6.1:4328165-4328184 MS.gene68005:CDS 45.0%
CGAAATGTGGCAATGAGAAC+TGG - chr6.1:4328133-4328152 MS.gene68005:CDS 45.0%
GGCTAACTCTTATCAACCGA+GGG + chr6.1:4327849-4327868 None:intergenic 45.0%
TATCAACCGAGGGCAAAGTA+GGG + chr6.1:4327839-4327858 None:intergenic 45.0%
TGGCTAACTCTTATCAACCG+AGG + chr6.1:4327850-4327869 None:intergenic 45.0%
TTAGTGGCTGAACCTTTGGT+TGG - chr6.1:4328273-4328292 MS.gene68005:CDS 45.0%
TTATCAACCGAGGGCAAAGT+AGG + chr6.1:4327840-4327859 None:intergenic 45.0%
TTGAAGAGTGGGAACTTGGA+GGG + chr6.1:4327688-4327707 None:intergenic 45.0%
TTTGAAGAGTGGGAACTTGG+AGG + chr6.1:4327689-4327708 None:intergenic 45.0%
TTTGATGCCGCAGTTGCAAA+TGG + chr6.1:4327992-4328011 None:intergenic 45.0%
! GTGGCAGCAACAACCTTTTT+AGG + chr6.1:4328051-4328070 None:intergenic 45.0%
! TGGAGGGTTATTGCTGTTGA+TGG + chr6.1:4327746-4327765 None:intergenic 45.0%
!! GTTGATGGTATCCATGTCGT+TGG + chr6.1:4327731-4327750 None:intergenic 45.0%
!! TCTTGTTGTTGTTGTGGTGG+TGG + chr6.1:4327808-4327827 None:intergenic 45.0%
!! TGCGAGCTATGTTTTGGACT+TGG - chr6.1:4328593-4328612 MS.gene68005:CDS 45.0%
ACATGGACACCACCAACCAA+AGG + chr6.1:4328288-4328307 None:intergenic 50.0%
AGAGGGTCATAATCGAGGAG+TGG + chr6.1:4327904-4327923 None:intergenic 50.0%
AGAGTTAGCCAAAACACGCG+TGG - chr6.1:4327859-4327878 MS.gene68005:CDS 50.0%
AGGACGTGGCCAAAACAAGT+TGG + chr6.1:4328661-4328680 None:intergenic 50.0%
CCATGTAGAGGGTGAACCTT+CGG - chr6.1:4328302-4328321 MS.gene68005:intron 50.0%
GAGGGTCATAATCGAGGAGT+GGG + chr6.1:4327903-4327922 None:intergenic 50.0%
GGTGATCCCCAACATGTCAA+TGG - chr6.1:4328158-4328177 MS.gene68005:CDS 50.0%
TCAGGAGAATCGGACAAAGG+CGG + chr6.1:4328633-4328652 None:intergenic 50.0%
TCGTTGGCGTCAGAAAGGAA+TGG + chr6.1:4327715-4327734 None:intergenic 50.0%
TGTTTGGAGAGGCGACAGAA+TGG + chr6.1:4327766-4327785 None:intergenic 50.0%
! GAACCGTGCGAGCTATGTTT+TGG - chr6.1:4328587-4328606 MS.gene68005:CDS 50.0%
!! GTTGGTGGTGTCCATGTAGA+GGG - chr6.1:4328291-4328310 MS.gene68005:intron 50.0%
!! GTTGTGGTGGTGGTTTGATG+AGG + chr6.1:4327798-4327817 None:intergenic 50.0%
!!! ACTTGTTTTGGCCACGTCCT+AGG - chr6.1:4328661-4328680 MS.gene68005:CDS 50.0%
!!! AGGGTTTTTTCCATGGCACG+TGG + chr6.1:4328070-4328089 None:intergenic 50.0%
AGGAGTGGGTGGAAGAATCG+AGG + chr6.1:4327889-4327908 None:intergenic 55.0%
CCATGTCGTTGGCGTCAGAA+AGG + chr6.1:4327720-4327739 None:intergenic 55.0%
CCGAAGGTTCACCCTCTACA+TGG + chr6.1:4328305-4328324 None:intergenic 55.0%
CCTTTCTGACGCCAACGACA+TGG - chr6.1:4327717-4327736 MS.gene68005:CDS 55.0%
GGTCATAATCGAGGAGTGGG+TGG + chr6.1:4327900-4327919 None:intergenic 55.0%
GTAGAGGGTGAACCTTCGGT+TGG - chr6.1:4328306-4328325 MS.gene68005:intron 55.0%
GTGGCTGAACCTTTGGTTGG+TGG - chr6.1:4328276-4328295 MS.gene68005:CDS 55.0%
TGGAGAGGCGACAGAATGGA+GGG + chr6.1:4327762-4327781 None:intergenic 55.0%
TTCTTCCGACCACCAACCGA+AGG + chr6.1:4328321-4328340 None:intergenic 55.0%
TTGGAGAGGCGACAGAATGG+AGG + chr6.1:4327763-4327782 None:intergenic 55.0%
! GAGGTTTGCCACGCGTGTTT+TGG + chr6.1:4327870-4327889 None:intergenic 55.0%
!! GGTTGGTGGTGTCCATGTAG+AGG - chr6.1:4328290-4328309 MS.gene68005:intron 55.0%
!! GTGAACCTTCGGTTGGTGGT+CGG - chr6.1:4328313-4328332 MS.gene68005:intron 55.0%
!!! TGTTTTGGCCACGTCCTAGG+CGG - chr6.1:4328664-4328683 MS.gene68005:CDS 55.0%
GAGGGTGAACCTTCGGTTGG+TGG - chr6.1:4328309-4328328 MS.gene68005:intron 60.0%
GTTGTTGCTGCCACGTGCCA+TGG - chr6.1:4328057-4328076 MS.gene68005:CDS 60.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 4327538 4328692 4327538 ID=MS.gene68005
chr6.1 mRNA 4327538 4328692 4327538 ID=MS.gene68005.t1;Parent=MS.gene68005
chr6.1 exon 4328461 4328692 4328461 ID=MS.gene68005.t1.exon1;Parent=MS.gene68005.t1
chr6.1 CDS 4328461 4328692 4328461 ID=cds.MS.gene68005.t1;Parent=MS.gene68005.t1
chr6.1 exon 4327538 4328295 4327538 ID=MS.gene68005.t1.exon2;Parent=MS.gene68005.t1
chr6.1 CDS 4327538 4328295 4327538 ID=cds.MS.gene68005.t1;Parent=MS.gene68005.t1
Gene Sequence

>MS.gene68005

ATGGCGGCGGTTGACTGGTCTAAACTTCCAACGGAGCTTCTCAATCTGATATCACAACGAATCGACGATGAAATCGATCTCATTCGCTTCCGATCAACTTGTTCAACATGGCGCTCTTCCTCCGTCCTAAAACTTCTTCAAATGTTACCCTCCAAGTTCCCACTCTTCAAATTACCATTCCTTTCTGACGCCAACGACATGGATACCATCAACAGCAATAACCCTCCATTCTGTCGCCTCTCCAAACAAAATCTCTACCTCATCAAACCACCACCACAACAACAACAAGAAGAACAAACCCTACTTTGCCCTCGGTTGATAAGAGTTAGCCAAAACACGCGTGGCAAACCTCGATTCTTCCACCCACTCCTCGATTATGACCCTCTTTCTTGTAAAGGTCTTGTGCTCGATTTTAACAAATTATCTGTCCTCCATTTGGGAAGCATCATTTCCATTTGCAACTGCGGCATCAAACCCAAAAATGAAGCCGACGATTGTTTCTACATATTCCCTAAAAAGGTTGTTGCTGCCACGTGCCATGGAAAAAACCCTCTCATTGTTGCCGCTTTAAATGCTCGCCCATTTCCGTTACTTTCGAAATGTGGCAATGAGAACTGGAAGGTGATCCCCAACATGTCAATGGTGTATGGAGACATATGCCTTTTTAAAGGGCACCCTTATGCGGTTGATGAAAATGGTAAGACAGTTATTGTTGGATCAGACTCAAATGTCCATTTAGTGGCTGAACCTTTGGTTGGTGGTGTCCATGTAGAGGGTGAACCTTCGGTTGGTGGTCGGAAGAATAAATTATTAGTGGAGAGCGATGGAGATTTGTTGTTAGCGGACGTTTATGTGCATGATGTAAGGGTTGATTTGTTCAAGCTTAATGAGAAGGAGAAGAAGTGGGTGAAGTTAACGAGTTTAGGGGATAGAGTTTTGTTCTTGGGGCTGAAATGTTCGTTTTCTATTTCAGCTTCAGATTTGTCTGTTGCCAAAGGGAATCGTGTGATCATTAGTGATGATATCTTAAGAGGATATTGTAGACGTAGGAACCGTGCGAGCTATGTTTTGGACTTGGATCAAGGTCAGCTTCCGCCTTTGTCCGATTCTCCTGAATATTCCAACTTGTTTTGGCCACGTCCTAGGCGGATCTGA
Protein sequence

>MS.gene68005.t1

MAAVDWSKLPTELLNLISQRIDDEIDLIRFRSTCSTWRSSSVLKLLQMLPSKFPLFKLPFLSDANDMDTINSNNPPFCLVLDFNKLSVLHLGSIISICNCGIKPKNEADDCFYIFPKKVVAATCHGKNPLIVAALNARPFPLLSKCGNENWKVIPNMSMVYGDICLFKGHPYAVDENGKTVIVGSDSNVHLVAEPLVGGVHVEGEPSVGGRKNKLLVESDGDLLLADVYVHDVRVDLFKLNEKEKKWVKLTSLGDRVLFLGLKCSFSISASDLSVAKGNRVIISDDILRGYCRRRNRASYVLDLDQGQLPPLSDSPEYSNLFWPRPRRI