AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68021


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68021.t1 MTR_1g096030 98.765 405 5 0 1 405 57 461 0.0 830
MS.gene68021.t1 MTR_7g027190 33.898 413 221 14 14 403 290 673 6.22e-64 217
MS.gene68021.t1 MTR_7g074650 32.718 379 222 10 29 403 443 792 1.93e-52 187
MS.gene68021.t1 MTR_4g102790 32.426 404 232 13 18 405 286 664 3.73e-52 185
MS.gene68021.t1 MTR_3g065980 31.463 410 222 15 18 403 166 540 1.20e-50 179
MS.gene68021.t1 MTR_5g097480 31.311 412 238 13 7 403 163 544 9.91e-49 173
MS.gene68021.t1 MTR_3g089055 28.967 397 238 12 23 403 197 565 6.94e-46 166
MS.gene68021.t1 MTR_3g089055 28.967 397 238 12 23 403 202 570 8.40e-46 166
MS.gene68021.t1 MTR_1g029420 29.723 397 235 12 23 403 224 592 9.27e-46 166
MS.gene68021.t1 MTR_4g104020 29.854 412 233 15 16 403 141 520 3.04e-45 164
MS.gene68021.t1 MTR_5g094450 29.064 406 234 14 21 403 158 532 5.64e-45 163
MS.gene68021.t1 MTR_4g077760 32.161 398 225 13 20 403 189 555 4.64e-44 161
MS.gene68021.t1 MTR_3g056110 28.894 398 241 13 21 403 172 542 1.62e-43 159
MS.gene68021.t1 MTR_1g069725 30.576 399 232 15 14 402 99 462 7.65e-43 156
MS.gene68021.t1 MTR_4g133660 30.348 402 233 15 19 402 170 542 1.03e-41 154
MS.gene68021.t1 MTR_5g058860 30.602 415 241 12 14 405 109 499 2.19e-41 153
MS.gene68021.t1 MTR_4g064200 30.025 403 237 13 16 402 276 649 5.38e-41 154
MS.gene68021.t1 MTR_3g072710 31.221 426 220 16 14 402 110 499 1.07e-40 151
MS.gene68021.t1 MTR_2g082090 30.549 419 226 16 14 403 197 579 1.36e-40 152
MS.gene68021.t1 MTR_4g076020 29.457 387 215 11 29 403 89 429 7.61e-40 147
MS.gene68021.t1 MTR_2g026250 30.159 378 210 14 47 403 252 596 1.28e-37 144
MS.gene68021.t1 MTR_8g442410 29.157 415 231 17 16 403 147 525 2.99e-37 142
MS.gene68021.t1 MTR_4g064150 29.750 400 238 14 21 402 358 732 1.65e-36 141
MS.gene68021.t1 MTR_4g064120 29.188 394 236 13 21 396 251 619 1.81e-35 138
MS.gene68021.t1 MTR_4g064180 29.640 361 222 12 21 363 251 597 1.08e-34 135
MS.gene68021.t1 MTR_2g097310 29.146 398 231 15 24 402 272 637 3.49e-34 134
MS.gene68021.t1 MTR_7g062120 27.204 397 244 14 24 403 372 740 9.66e-34 133
MS.gene68021.t1 MTR_2g097467 26.087 414 259 13 5 402 271 653 5.09e-33 131
MS.gene68021.t1 MTR_2g097410 26.895 409 244 14 17 402 364 740 8.24e-33 130
MS.gene68021.t1 MTR_2g097463 26.263 396 250 11 21 402 287 654 1.22e-31 127
MS.gene68021.t1 MTR_2g097473 25.758 396 249 12 21 402 289 653 6.80e-31 125
MS.gene68021.t1 MTR_4g064160 28.173 394 242 15 24 402 316 683 2.80e-30 123
MS.gene68021.t1 MTR_2g097390 27.157 394 244 13 24 402 275 640 7.75e-30 122
MS.gene68021.t1 MTR_2g034260 25.598 418 260 13 9 403 195 584 8.55e-30 121
MS.gene68021.t1 MTR_2g097350 27.411 394 243 15 24 402 274 639 8.54e-29 119
MS.gene68021.t1 MTR_4g097080 25.495 404 246 14 23 403 132 503 2.28e-28 116
MS.gene68021.t1 MTR_2g034250 25.542 415 265 12 9 403 195 585 6.27e-28 116
MS.gene68021.t1 MTR_8g093070 30.303 396 226 17 21 402 136 495 2.21e-27 114
MS.gene68021.t1 MTR_3g022830 23.266 447 259 12 15 403 16 436 3.07e-27 112
MS.gene68021.t1 MTR_2g034280 25.647 425 249 15 9 403 188 575 3.49e-27 114
MS.gene68021.t1 MTR_5g015490 27.451 408 239 15 23 403 113 490 4.56e-27 112
MS.gene68021.t1 MTR_2g097380 30.488 328 177 16 31 340 231 525 5.62e-27 113
MS.gene68021.t1 MTR_7g109580 25.845 414 255 14 11 405 176 556 1.67e-26 111
MS.gene68021.t1 MTR_0092s0100 26.368 402 235 16 21 403 369 728 2.60e-20 93.6
MS.gene68021.t1 MTR_0092s0100 26.368 402 235 16 21 403 372 731 2.72e-20 93.6
MS.gene68021.t1 MTR_6g047750 26.895 409 227 15 25 403 296 662 6.37e-20 92.4
MS.gene68021.t1 MTR_4g076140 28.889 225 150 4 15 237 39 255 2.63e-19 90.1
MS.gene68021.t1 MTR_4g076140 31.325 166 96 4 239 404 323 470 5.26e-18 85.9
MS.gene68021.t1 MTR_7g057230 26.263 396 240 13 25 402 295 656 2.81e-19 90.5
MS.gene68021.t1 MTR_5g009080 27.473 273 160 9 15 280 47 288 3.22e-19 89.7
MS.gene68021.t1 MTR_5g009080 29.070 172 104 4 233 404 326 479 9.10e-19 88.6
MS.gene68021.t1 MTR_5g015950 23.333 450 252 14 20 402 22 445 9.22e-19 88.2
MS.gene68021.t1 MTR_3g053270 23.333 450 252 14 20 402 22 445 9.22e-19 88.2
MS.gene68021.t1 MTR_2g089100 21.362 426 267 12 17 402 45 442 1.35e-18 87.8
MS.gene68021.t1 MTR_2g099110 24.590 366 240 11 55 403 105 451 2.31e-17 84.0
MS.gene68021.t1 MTR_8g020840 23.227 409 256 14 23 403 170 548 1.40e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68021.t1 AT3G54220 35.323 402 212 12 14 402 283 649 1.78e-55 194
MS.gene68021.t1 AT1G14920 32.767 412 225 16 7 403 155 529 8.58e-54 187
MS.gene68021.t1 AT2G04890 32.648 389 225 12 24 403 53 413 1.96e-52 181
MS.gene68021.t1 AT3G03450 32.587 402 222 16 18 403 177 545 6.73e-51 179
MS.gene68021.t1 AT5G41920 32.108 408 229 12 10 404 29 401 1.18e-50 176
MS.gene68021.t1 AT1G21450 30.982 397 231 12 23 403 224 593 1.15e-49 177
MS.gene68021.t1 AT2G01570 30.941 404 223 16 17 403 217 581 1.19e-49 177
MS.gene68021.t1 AT5G17490 32.039 412 224 18 8 403 145 516 1.23e-48 173
MS.gene68021.t1 AT1G66350 31.078 399 221 16 18 403 149 506 1.51e-47 170
MS.gene68021.t1 AT5G48150 30.380 395 232 13 25 403 123 490 3.92e-47 168
MS.gene68021.t1 AT5G48150 30.380 395 232 13 25 403 123 490 3.92e-47 168
MS.gene68021.t1 AT5G48150 30.380 395 232 13 25 403 123 490 3.92e-47 168
MS.gene68021.t1 AT5G48150 30.380 395 232 13 25 403 123 490 3.92e-47 168
MS.gene68021.t1 AT1G55580 30.841 428 228 15 21 403 41 445 4.84e-47 167
MS.gene68021.t1 AT1G63100 29.381 388 227 11 43 404 264 630 1.65e-46 169
MS.gene68021.t1 AT1G63100 29.381 388 227 11 43 404 289 655 2.48e-46 169
MS.gene68021.t1 AT5G66770 32.658 395 229 14 20 403 216 584 1.72e-45 166
MS.gene68021.t1 AT4G08250 31.127 408 238 14 14 404 97 478 7.58e-43 156
MS.gene68021.t1 AT3G50650 33.155 374 214 12 42 403 193 542 3.22e-42 156
MS.gene68021.t1 AT1G50600 29.873 395 235 12 24 403 159 526 5.80e-42 155
MS.gene68021.t1 AT1G50600 29.873 395 235 12 24 403 234 601 2.33e-41 154
MS.gene68021.t1 AT1G50600 29.873 395 235 12 24 403 230 597 2.45e-41 154
MS.gene68021.t1 AT4G17230 29.777 403 235 12 19 403 153 525 1.90e-37 142
MS.gene68021.t1 AT4G17230 29.777 403 235 12 19 403 153 525 1.90e-37 142
MS.gene68021.t1 AT1G50420 27.015 459 238 17 15 403 48 479 3.76e-32 127
MS.gene68021.t1 AT2G29060 27.456 397 250 13 24 405 284 657 4.74e-32 128
MS.gene68021.t1 AT2G29060 27.456 397 250 13 24 405 321 694 5.34e-32 128
MS.gene68021.t1 AT2G37650 25.628 398 253 13 21 403 332 701 2.76e-30 123
MS.gene68021.t1 AT2G37650 25.628 398 253 13 21 403 344 713 3.95e-30 123
MS.gene68021.t1 AT2G29065 25.980 408 241 16 24 402 252 627 1.91e-29 120
MS.gene68021.t1 AT1G07530 26.396 394 250 14 24 402 396 764 1.66e-28 118
MS.gene68021.t1 AT3G46600 26.263 396 250 13 23 403 3 371 2.68e-28 114
MS.gene68021.t1 AT3G46600 26.329 395 251 13 23 403 211 579 1.33e-27 115
MS.gene68021.t1 AT3G46600 26.329 395 251 13 23 403 179 547 1.68e-27 114
MS.gene68021.t1 AT1G07520 25.187 401 252 15 24 402 154 528 1.92e-27 114
MS.gene68021.t1 AT1G07520 25.187 401 252 15 24 402 154 528 1.92e-27 114
MS.gene68021.t1 AT1G07520 25.187 401 252 15 24 402 319 693 2.65e-27 114
MS.gene68021.t1 AT1G07520 25.187 401 252 15 24 402 318 692 3.07e-27 114
MS.gene68021.t1 AT5G59450 25.369 406 259 13 16 403 219 598 3.29e-27 114
MS.gene68021.t1 AT3G60630 29.755 326 179 13 96 403 329 622 4.03e-26 110
MS.gene68021.t1 AT4G37650 23.716 409 258 13 24 403 146 529 4.95e-22 98.6
MS.gene68021.t1 AT2G45160 28.682 387 208 18 41 403 297 639 1.09e-21 97.8
MS.gene68021.t1 AT5G52510 25.679 405 242 16 25 403 269 640 1.23e-21 97.4
MS.gene68021.t1 AT4G00150 27.446 368 208 17 49 403 233 554 4.83e-21 95.5
MS.gene68021.t1 AT4G36710 24.510 408 263 14 9 403 110 485 3.09e-20 92.8
MS.gene68021.t1 AT3G49950 23.058 412 252 13 23 402 29 407 2.41e-19 89.7

Find 0 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATGAAAAAATGGAAACA+TGG - 5087:8463-8482 MS.gene68021:CDS 20.0%
!! AATCAATAACATGAATAGAA+CGG + 5087:7885-7904 MS.gene68021:intergenic 20.0%
!! GATCAAGAGTAATTAATTTA+AGG - 5087:8103-8122 MS.gene68021:CDS 20.0%
!! TTGTGCAAAATATGAAAAAA+TGG - 5087:8455-8474 MS.gene68021:CDS 20.0%
!!! ACAACTCATTTTGTTTAAAT+AGG + 5087:8166-8185 MS.gene68021:intergenic 20.0%
!!! ATTGATTTTGATGTTTCATA+TGG - 5087:7898-7917 MS.gene68021:CDS 20.0%
! ATTGAAAATAACTGACACAT+TGG - 5087:8188-8207 MS.gene68021:CDS 25.0%
! TTGAAAATAACTGACACATT+GGG - 5087:8189-8208 MS.gene68021:CDS 25.0%
!! AAATGAGCAAATTGGTAATA+CGG + 5087:7808-7827 MS.gene68021:intergenic 25.0%
!! ATGTTTCATATGGTTTTCAA+TGG - 5087:7908-7927 MS.gene68021:CDS 25.0%
!!! AACATTGTTTTTGAGTTTCA+AGG - 5087:8069-8088 MS.gene68021:CDS 25.0%
!!! TTTCTCTTCTTCTTTTTCAA+AGG + 5087:7857-7876 MS.gene68021:intergenic 25.0%
AAAAGTAGAATGGAGAATCA+TGG - 5087:8486-8505 MS.gene68021:CDS 30.0%
AAGGTTAAGAATTGAGAAGA+AGG - 5087:8371-8390 MS.gene68021:CDS 30.0%
ACCATTCTATGAAATGTTGA+TGG - 5087:7726-7745 MS.gene68021:CDS 30.0%
AGAATCTCTTTCAGATTAAC+TGG - 5087:7976-7995 MS.gene68021:CDS 30.0%
CTCAACATTGAAGATTCAAA+TGG - 5087:7523-7542 MS.gene68021:CDS 30.0%
GAAGAAGAAAAATGAAACTG+TGG - 5087:8125-8144 MS.gene68021:CDS 30.0%
GTGAGAAGTAAGTGAATTAA+TGG + 5087:7553-7572 MS.gene68021:intergenic 30.0%
TCCATCAACATTTCATAGAA+TGG + 5087:7730-7749 MS.gene68021:intergenic 30.0%
TTATTGTCCACTTCAATTTG+AGG - 5087:8578-8597 MS.gene68021:CDS 30.0%
TTGTTCAACAACAACAACAA+TGG + 5087:8238-8257 MS.gene68021:intergenic 30.0%
! AATAACTATGATGATGTGGA+TGG - 5087:8423-8442 MS.gene68021:CDS 30.0%
! AGAATTGAGAAGAAGGTTTT+TGG - 5087:8378-8397 MS.gene68021:CDS 30.0%
! AGGTTAGTTAGTTTCTCAAA+AGG - 5087:8039-8058 MS.gene68021:CDS 30.0%
! GCTTAATAACTATGATGATG+TGG - 5087:8419-8438 MS.gene68021:CDS 30.0%
! TCTTTCAGATTAACTGGTTT+CGG - 5087:7982-8001 MS.gene68021:CDS 30.0%
! TTCTGTTGATGAAAACAACT+TGG - 5087:7582-7601 MS.gene68021:CDS 30.0%
!! GTTAGTTTCTCAAAAGGTTT+TGG - 5087:8045-8064 MS.gene68021:CDS 30.0%
!!! AGAATGGTGATTTTTTTGTG+AGG + 5087:7714-7733 MS.gene68021:intergenic 30.0%
AACCTTTCAATACTCTCAAG+TGG + 5087:8357-8376 MS.gene68021:intergenic 35.0%
AGCTTCAAGAAACTGAATCT+AGG - 5087:8019-8038 MS.gene68021:CDS 35.0%
ATGGAGAATCATGGATTTGT+TGG - 5087:8495-8514 MS.gene68021:CDS 35.0%
CAACAACAACAACAATGGAA+GGG + 5087:8233-8252 MS.gene68021:intergenic 35.0%
CTGTTTGGTAAAGATCTGTT+AGG + 5087:7624-7643 MS.gene68021:intergenic 35.0%
CTTGATAAGAAGGTTCTACT+AGG + 5087:8276-8295 MS.gene68021:intergenic 35.0%
CTTTCAGAAAAAGCAACAAG+TGG - 5087:7949-7968 MS.gene68021:CDS 35.0%
GAAAGTGATTGAATCAGTGA+AGG + 5087:7934-7953 MS.gene68021:intergenic 35.0%
GAAGAAAAATGAAACTGTGG+CGG - 5087:8128-8147 MS.gene68021:CDS 35.0%
GGAAACATGGAAAAGTAGAA+TGG - 5087:8476-8495 MS.gene68021:CDS 35.0%
GTCTGTGAATCTTGATAAGA+AGG + 5087:8286-8305 MS.gene68021:intergenic 35.0%
TCAACAACAACAACAATGGA+AGG + 5087:8234-8253 MS.gene68021:intergenic 35.0%
TTGCTGTGAAATGAGCAAAT+TGG + 5087:7816-7835 MS.gene68021:intergenic 35.0%
! GAGTTTTGCAGTTAAACCAT+CGG + 5087:7692-7711 MS.gene68021:intergenic 35.0%
! GTTGTTGTTGTTGAACAAGA+AGG - 5087:8240-8259 MS.gene68021:CDS 35.0%
!! GAGTTTCAAGGTTTGTTAAG+AGG - 5087:8081-8100 MS.gene68021:CDS 35.0%
!! TACCACTTGAGAGTATTGAA+AGG - 5087:8352-8371 MS.gene68021:CDS 35.0%
!! TCTCTTTAGGATGGCAAAAT+AGG - 5087:8652-8671 MS.gene68021:CDS 35.0%
!! TTCAGAAAGAGATGATGGAA+GGG - 5087:8626-8645 MS.gene68021:CDS 35.0%
!! TTTCAGAAAGAGATGATGGA+AGG - 5087:8625-8644 MS.gene68021:CDS 35.0%
AGGGTTTCAGAAAGAGATGA+TGG - 5087:8621-8640 MS.gene68021:CDS 40.0%
CACAGCAAATCAAGCTATCT+TGG - 5087:7828-7847 MS.gene68021:CDS 40.0%
CCACTTCAATTTGAGGAAGA+AGG - 5087:8585-8604 MS.gene68021:CDS 40.0%
CCTTCTTCCTCAAATTGAAG+TGG + 5087:8588-8607 MS.gene68021:intergenic 40.0%
CTTCAATTTGAGGAAGAAGG+TGG - 5087:8588-8607 MS.gene68021:CDS 40.0%
! AACTATGATGATGTGGATGG+TGG - 5087:8426-8445 MS.gene68021:CDS 40.0%
! AGAAACTCTTCGTCGATTGT+TGG + 5087:7757-7776 MS.gene68021:intergenic 40.0%
! TTTTGCTTACTGTGTCAGCA+TGG - 5087:8676-8695 MS.gene68021:CDS 40.0%
CAATTTGAGGAAGAAGGTGG+TGG - 5087:8591-8610 MS.gene68021:CDS 45.0%
GACACGTTGAACTGAATCAC+CGG + 5087:7656-7675 MS.gene68021:intergenic 45.0%
GATGGAAGGGCTATCTCTTT+AGG - 5087:8639-8658 MS.gene68021:CDS 45.0%
TACCAAACAGTTTCCCTCAC+CGG - 5087:7634-7653 MS.gene68021:CDS 45.0%
TTGAACTGAATCACCGGTGA+GGG + 5087:7650-7669 MS.gene68021:intergenic 45.0%
! GAAGGGCTATCTCTTTAGGA+TGG - 5087:8643-8662 MS.gene68021:CDS 45.0%
GTCGTCGCATATTTCACCGA+TGG - 5087:7673-7692 MS.gene68021:CDS 50.0%
GTTGAACTGAATCACCGGTG+AGG + 5087:7651-7670 MS.gene68021:intergenic 50.0%
! TTTGAGGAAGAAGGTGGTGG+TGG - 5087:8594-8613 MS.gene68021:CDS 50.0%
!! AAGAAGGTGGTGGTGGTTTC+AGG - 5087:8601-8620 MS.gene68021:CDS 50.0%
!! AGAAGGTGGTGGTGGTTTCA+GGG - 5087:8602-8621 MS.gene68021:CDS 50.0%
CACCGGTGAGGGAAACTGTT+TGG + 5087:7639-7658 MS.gene68021:intergenic 55.0%


Chromosome Type Strat End Strand Name
5087 gene 7493 8710 7493 ID=MS.gene68021
5087 mRNA 7493 8710 7493 ID=MS.gene68021.t1;Parent=MS.gene68021
5087 exon 7493 8710 7493 ID=MS.gene68021.t1.exon1;Parent=MS.gene68021.t1
5087 CDS 7493 8710 7493 ID=cds.MS.gene68021.t1;Parent=MS.gene68021.t1
Gene Sequence

>MS.gene68021

ATGAGAGAACAAATGCTAAGAAAAAGCACTCTCAACATTGAAGATTCAAATGGTCTTCCATTAATTCACTTACTTCTCACAACAGCAACTTCTGTTGATGAAAACAACTTGGATTCATCTCTTGAAAACCTAACAGATCTTTACCAAACAGTTTCCCTCACCGGTGATTCAGTTCAACGTGTCGTCGCATATTTCACCGATGGTTTAACTGCAAAACTCCTCACAAAAAAATCACCATTCTATGAAATGTTGATGGAAGAACCAACAATCGACGAAGAGTTTCTCGCATTCACAGATCTCTACAGAGTTTCACCGTATTACCAATTTGCTCATTTCACAGCAAATCAAGCTATCTTGGAAGCCTTTGAAAAAGAAGAAGAGAAAAATAACCGTTCTATTCATGTTATTGATTTTGATGTTTCATATGGTTTTCAATGGCCTTCACTGATTCAATCACTTTCAGAAAAAGCAACAAGTGGCAACAGAATCTCTTTCAGATTAACTGGTTTCGGTAAGAATTTAAAAGAGCTTCAAGAAACTGAATCTAGGTTAGTTAGTTTCTCAAAAGGTTTTGGTAACATTGTTTTTGAGTTTCAAGGTTTGTTAAGAGGATCAAGAGTAATTAATTTAAGGAAGAAGAAAAATGAAACTGTGGCGGTTAATTTAGTTTCCTATTTAAACAAAATGAGTTGTTTATTGAAAATAACTGACACATTGGGATTTGTTCATTCTCTTAACCCTTCCATTGTTGTTGTTGTTGAACAAGAAGGTAGTAAAAATCCTAGTAGAACCTTCTTATCAAGATTCACAGACACATTGCATTATTTTGCAGCTATGTTTGATTCACTTGATGATTGTTTACCACTTGAGAGTATTGAAAGGTTAAGAATTGAGAAGAAGGTTTTTGGTAAAGAGATTAAAAGCATGCTTAATAACTATGATGATGTGGATGGTGGTGTTGATTGTGCAAAATATGAAAAAATGGAAACATGGAAAAGTAGAATGGAGAATCATGGATTTGTTGGTATGAAAATGAGTTCAAAATGTTTAATACAAGCAAAATTGTTGCTTAAGATGAGAACACATTATTGTCCACTTCAATTTGAGGAAGAAGGTGGTGGTGGTTTCAGGGTTTCAGAAAGAGATGATGGAAGGGCTATCTCTTTAGGATGGCAAAATAGGTTTTTGCTTACTGTGTCAGCATGGCAATCACTTTGA

Protein sequence

>MS.gene68021.t1

MREQMLRKSTLNIEDSNGLPLIHLLLTTATSVDENNLDSSLENLTDLYQTVSLTGDSVQRVVAYFTDGLTAKLLTKKSPFYEMLMEEPTIDEEFLAFTDLYRVSPYYQFAHFTANQAILEAFEKEEEKNNRSIHVIDFDVSYGFQWPSLIQSLSEKATSGNRISFRLTGFGKNLKELQETESRLVSFSKGFGNIVFEFQGLLRGSRVINLRKKKNETVAVNLVSYLNKMSCLLKITDTLGFVHSLNPSIVVVVEQEGSKNPSRTFLSRFTDTLHYFAAMFDSLDDCLPLESIERLRIEKKVFGKEIKSMLNNYDDVDGGVDCAKYEKMETWKSRMENHGFVGMKMSSKCLIQAKLLLKMRTHYCPLQFEEEGGGGFRVSERDDGRAISLGWQNRFLLTVSAWQSL