AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68327

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68327.t1 MTR_5g035150 95.862 435 18 0 1 435 1 435 0.0 852
MS.gene68327.t1 MTR_5g035070 65.287 435 149 2 1 435 5 437 0.0 566
MS.gene68327.t1 MTR_5g035090 62.759 435 156 3 1 435 3 431 0.0 550
MS.gene68327.t1 MTR_5g035080 66.914 405 114 6 34 435 37 424 0.0 541
MS.gene68327.t1 MTR_3g109840 62.385 436 137 9 1 433 6 417 0.0 531
MS.gene68327.t1 MTR_8g104970 57.359 462 147 9 16 432 10 466 0.0 518
MS.gene68327.t1 MTR_7g033230 61.972 355 128 4 82 433 261 611 2.93e-161 468
MS.gene68327.t1 MTR_7g033230 61.972 355 128 4 82 433 331 681 2.54e-160 468
MS.gene68327.t1 MTR_5g035140 66.792 265 81 4 172 433 7 267 2.50e-129 374
MS.gene68327.t1 MTR_8g099300 42.614 352 191 7 85 431 74 419 1.78e-97 298
MS.gene68327.t1 MTR_8g027900 39.096 376 214 6 60 431 191 555 8.51e-96 298
MS.gene68327.t1 MTR_6g016880 37.831 415 240 10 25 432 17 420 3.05e-94 291
MS.gene68327.t1 MTR_7g019120 36.077 413 242 10 29 432 22 421 5.66e-89 277
MS.gene68327.t1 MTR_6g016875 38.544 371 214 7 68 432 47 409 3.54e-84 264
MS.gene68327.t1 MTR_6g007777 38.172 372 212 8 66 433 102 459 7.52e-84 265
MS.gene68327.t1 MTR_2g016060 37.360 356 198 12 90 433 60 402 8.88e-76 242
MS.gene68327.t1 MTR_1g076690 34.160 363 212 11 85 433 223 572 3.72e-66 222
MS.gene68327.t1 MTR_6g061710 32.258 372 211 11 83 433 165 516 1.36e-64 216
MS.gene68327.t1 MTR_7g094750 33.516 364 209 13 84 433 90 434 5.19e-53 184
MS.gene68327.t1 MTR_2g022560 34.225 374 196 16 84 435 96 441 2.34e-50 177
MS.gene68327.t1 MTR_5g035120 49.412 170 75 3 4 173 5 163 5.97e-47 159
MS.gene68327.t1 MTR_0136s0010 32.514 366 207 14 85 433 170 512 7.56e-47 169
MS.gene68327.t1 MTR_4g126950 32.044 362 211 13 83 431 107 446 3.98e-46 166
MS.gene68327.t1 MTR_7g093450 31.358 405 235 15 43 427 202 583 1.54e-45 167
MS.gene68327.t1 MTR_8g069790 29.867 375 226 13 73 432 166 518 8.38e-43 158
MS.gene68327.t1 MTR_1g058380 29.459 370 212 15 83 431 53 394 3.47e-42 154
MS.gene68327.t1 MTR_7g109550 31.549 355 203 13 85 427 137 463 3.79e-42 155
MS.gene68327.t1 MTR_5g059400 30.303 363 209 14 84 427 174 511 3.80e-42 156
MS.gene68327.t1 MTR_1g116130 30.303 363 209 14 84 427 174 511 3.80e-42 156
MS.gene68327.t1 MTR_4g125060 30.245 367 218 12 76 427 124 467 4.81e-42 155
MS.gene68327.t1 MTR_5g071350 29.839 372 214 10 77 427 50 395 5.28e-42 153
MS.gene68327.t1 MTR_8g093530 29.609 358 214 10 83 431 88 416 5.97e-42 154
MS.gene68327.t1 MTR_4g014440 31.111 360 213 16 85 427 62 403 8.86e-42 153
MS.gene68327.t1 MTR_4g125060 30.245 367 218 12 76 427 163 506 1.13e-41 154
MS.gene68327.t1 MTR_4g125060 30.245 367 218 12 76 427 192 535 1.62e-41 154
MS.gene68327.t1 MTR_4g121560 27.594 424 254 17 27 431 64 453 2.73e-41 152
MS.gene68327.t1 MTR_7g070060 30.748 361 213 15 85 427 64 405 9.15e-41 150
MS.gene68327.t1 MTR_8g463790 27.294 425 258 15 23 427 12 405 3.00e-40 149
MS.gene68327.t1 MTR_5g033800 27.446 368 229 12 81 433 141 485 2.37e-39 148
MS.gene68327.t1 MTR_3g035830 30.084 359 194 11 83 427 29 344 2.80e-39 144
MS.gene68327.t1 MTR_4g016940 28.457 376 219 16 79 431 79 427 3.68e-39 146
MS.gene68327.t1 MTR_2g073510 31.549 355 207 13 82 431 30 353 6.53e-39 144
MS.gene68327.t1 MTR_7g068920 30.311 386 217 15 69 431 65 421 1.49e-38 144
MS.gene68327.t1 MTR_3g021090 30.663 362 188 14 83 427 29 344 1.11e-37 142
MS.gene68327.t1 MTR_3g464570 30.086 349 225 11 82 427 40 372 1.22e-37 141
MS.gene68327.t1 MTR_3g090120 28.831 385 214 13 69 431 113 459 6.43e-37 140
MS.gene68327.t1 MTR_3g021060 30.726 358 193 12 83 427 44 359 9.98e-37 138
MS.gene68327.t1 MTR_3g070900 28.056 360 228 12 82 427 103 445 1.29e-36 139
MS.gene68327.t1 MTR_3g049330 30.919 359 208 13 76 427 21 346 1.91e-36 137
MS.gene68327.t1 MTR_6g016240 28.027 446 266 16 8 431 4 416 3.57e-36 138
MS.gene68327.t1 MTR_2g073500 30.726 358 202 14 82 431 26 345 5.03e-36 135
MS.gene68327.t1 MTR_7g094750 33.871 248 145 7 84 321 90 328 2.02e-35 134
MS.gene68327.t1 MTR_8g093870 27.273 363 229 9 83 431 131 472 2.07e-35 137
MS.gene68327.t1 MTR_4g088030 30.347 346 208 10 84 427 32 346 4.14e-35 133
MS.gene68327.t1 MTR_5g083260 29.919 371 205 13 82 427 80 420 4.30e-35 135
MS.gene68327.t1 MTR_4g092830 28.537 410 227 16 54 431 17 392 6.74e-35 134
MS.gene68327.t1 MTR_3g071650 30.000 360 213 12 85 427 89 426 1.35e-34 134
MS.gene68327.t1 MTR_2g091175 31.988 347 198 13 85 427 50 362 4.02e-34 131
MS.gene68327.t1 MTR_4g087730 30.347 346 208 10 84 427 44 358 5.65e-34 130
MS.gene68327.t1 MTR_1g050448 27.989 368 233 12 67 431 37 375 3.91e-33 128
MS.gene68327.t1 MTR_1g112210 26.776 366 209 13 80 427 54 378 7.53e-33 128
MS.gene68327.t1 MTR_5g034810 29.429 350 208 13 83 427 33 348 8.95e-33 127
MS.gene68327.t1 MTR_4g079700 28.941 387 239 13 49 431 9 363 3.31e-32 125
MS.gene68327.t1 MTR_2g015720 30.220 364 218 14 70 431 29 358 7.27e-32 125
MS.gene68327.t1 MTR_5g082880 28.493 365 221 15 83 427 163 507 3.76e-31 125
MS.gene68327.t1 MTR_1g112210 25.627 359 209 11 80 427 54 365 1.36e-30 121
MS.gene68327.t1 MTR_4g014430 27.989 368 219 15 83 431 106 446 1.02e-29 120
MS.gene68327.t1 MTR_7g019140 37.333 150 85 3 29 173 22 167 1.63e-28 110
MS.gene68327.t1 MTR_1g111010 28.219 365 213 15 73 431 20 341 4.41e-28 114
MS.gene68327.t1 MTR_5g033800 31.667 180 116 3 81 254 141 319 6.80e-27 111
MS.gene68327.t1 MTR_7g070060 28.896 308 183 14 137 427 15 303 6.39e-25 104
MS.gene68327.t1 MTR_7g070060 31.301 246 144 9 85 319 64 295 2.21e-24 103
MS.gene68327.t1 MTR_4g102570 40.157 127 64 3 201 321 2 122 8.62e-23 94.7
MS.gene68327.t1 MTR_3g464570 28.369 282 183 11 149 427 3 268 4.21e-21 93.2
MS.gene68327.t1 MTR_7g117160 33.514 185 71 7 246 430 57 189 1.05e-19 87.0
MS.gene68327.t1 MTR_7g019130 32.000 125 78 3 206 326 4 125 2.91e-13 67.4
MS.gene68327.t1 MTR_0929s0010 31.818 110 66 3 29 133 22 127 1.46e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68327.t1 AT1G70230 54.494 356 144 6 82 435 76 415 2.02e-148 428
MS.gene68327.t1 AT5G15890 40.052 387 213 8 50 432 152 523 4.83e-99 306
MS.gene68327.t1 AT1G01430 39.276 387 220 8 52 431 69 447 3.26e-93 289
MS.gene68327.t1 AT4G01080 38.442 398 222 8 35 429 51 428 5.20e-92 285
MS.gene68327.t1 AT3G02440 39.045 356 196 7 85 432 127 469 6.44e-91 283
MS.gene68327.t1 AT3G02440 39.045 356 196 7 85 432 135 477 6.50e-91 284
MS.gene68327.t1 AT4G23790 36.449 428 259 9 10 431 7 427 1.15e-90 281
MS.gene68327.t1 AT3G28150 36.855 407 235 10 36 433 19 412 1.97e-90 280
MS.gene68327.t1 AT4G11090 36.343 432 254 12 9 431 6 425 3.27e-88 275
MS.gene68327.t1 AT5G15900 37.203 379 220 10 65 435 45 413 2.29e-82 260
MS.gene68327.t1 AT5G15900 37.203 379 220 10 65 435 64 432 3.95e-82 260
MS.gene68327.t1 AT4G25360 33.671 395 233 11 53 433 147 526 1.30e-70 232
MS.gene68327.t1 AT4G25360 33.671 395 233 11 53 433 147 526 1.30e-70 232
MS.gene68327.t1 AT5G51640 34.615 390 222 14 63 433 115 490 4.76e-66 219
MS.gene68327.t1 AT5G19160 34.426 366 209 11 85 435 99 448 1.13e-55 191
MS.gene68327.t1 AT3G06080 32.787 366 212 13 85 435 103 449 2.81e-51 179
MS.gene68327.t1 AT3G12060 32.418 364 213 14 77 427 188 531 3.00e-49 176
MS.gene68327.t1 AT1G48880 34.218 377 194 17 71 428 95 436 2.85e-46 166
MS.gene68327.t1 AT5G64020 31.622 370 212 14 85 431 57 408 2.94e-46 165
MS.gene68327.t1 AT5G06700 31.933 357 212 13 83 427 253 590 1.16e-45 167
MS.gene68327.t1 AT3G11570 32.172 373 195 15 85 431 79 419 2.03e-44 160
MS.gene68327.t1 AT5G20590 31.536 371 210 14 75 427 131 475 1.20e-43 159
MS.gene68327.t1 AT2G14530 29.524 420 235 19 36 431 24 406 6.74e-43 156
MS.gene68327.t1 AT5G58600 29.843 382 214 11 68 431 55 400 6.32e-42 153
MS.gene68327.t1 AT3G62390 29.834 362 207 13 83 427 136 467 8.42e-42 154
MS.gene68327.t1 AT3G14850 32.591 359 204 11 79 431 28 354 2.01e-40 148
MS.gene68327.t1 AT5G06230 30.563 373 208 15 83 431 19 364 5.74e-40 147
MS.gene68327.t1 AT5G49340 29.014 355 225 11 84 427 99 437 6.95e-40 149
MS.gene68327.t1 AT5G06230 30.563 373 208 15 83 431 60 405 9.84e-40 147
MS.gene68327.t1 AT2G38320 29.558 362 226 13 80 427 54 400 2.51e-39 146
MS.gene68327.t1 AT2G31110 30.471 361 213 15 72 427 31 358 8.02e-39 144
MS.gene68327.t1 AT2G37720 29.348 368 215 11 83 431 141 482 1.33e-37 143
MS.gene68327.t1 AT2G30010 28.191 376 212 10 82 431 53 396 1.54e-37 141
MS.gene68327.t1 AT2G30010 28.800 375 207 12 84 431 98 439 2.09e-37 142
MS.gene68327.t1 AT5G20680 26.952 397 246 12 57 431 83 457 2.58e-37 142
MS.gene68327.t1 AT1G60790 27.248 367 230 13 83 432 188 534 4.64e-37 142
MS.gene68327.t1 AT5G20680 26.952 397 246 12 57 431 159 533 6.69e-37 142
MS.gene68327.t1 AT5G64470 28.691 359 225 13 85 431 55 394 7.61e-37 139
MS.gene68327.t1 AT5G20680 26.952 397 246 12 57 431 177 551 8.90e-37 142
MS.gene68327.t1 AT5G20680 26.952 397 246 12 57 431 177 551 8.90e-37 142
MS.gene68327.t1 AT5G20680 26.952 397 246 12 57 431 177 551 8.90e-37 142
MS.gene68327.t1 AT5G20680 26.952 397 246 12 57 431 177 551 8.90e-37 142
MS.gene68327.t1 AT2G30900 30.028 353 209 12 82 427 40 361 1.10e-36 138
MS.gene68327.t1 AT5G19160 36.992 246 136 9 85 320 99 335 2.77e-36 136
MS.gene68327.t1 AT5G01360 27.978 361 226 13 83 428 34 375 5.75e-36 136
MS.gene68327.t1 AT1G73140 28.841 371 224 15 72 427 56 401 1.33e-35 136
MS.gene68327.t1 AT3G55990 29.363 361 220 14 83 427 138 479 1.74e-35 137
MS.gene68327.t1 AT5G01360 27.978 361 226 13 83 428 90 431 2.08e-35 136
MS.gene68327.t1 AT2G42570 30.899 356 199 15 85 431 47 364 2.35e-35 134
MS.gene68327.t1 AT2G38320 30.061 326 200 12 115 427 7 317 2.90e-35 133
MS.gene68327.t1 AT5G64470 28.412 359 232 13 85 431 55 400 6.72e-35 134
MS.gene68327.t1 AT3G11030 27.933 358 228 11 83 427 104 444 9.95e-35 134
MS.gene68327.t1 AT1G29050 29.167 360 220 12 75 431 51 378 1.08e-34 133
MS.gene68327.t1 AT2G40320 28.962 366 215 13 82 427 78 418 1.59e-34 133
MS.gene68327.t1 AT2G40160 27.765 425 257 17 26 427 22 419 2.33e-34 133
MS.gene68327.t1 AT2G40160 26.995 426 259 16 26 427 22 419 2.77e-34 133
MS.gene68327.t1 AT2G34070 29.379 354 210 14 84 431 64 383 8.22e-34 130
MS.gene68327.t1 AT2G40150 28.571 385 233 16 63 427 54 416 3.03e-33 130
MS.gene68327.t1 AT3G54260 28.729 362 205 15 85 431 51 374 1.06e-32 127
MS.gene68327.t1 AT3G06080 32.800 250 143 8 85 321 103 340 3.49e-32 125
MS.gene68327.t1 AT1G78710 29.917 361 215 15 76 431 29 356 1.71e-30 121
MS.gene68327.t1 AT3G62390 29.675 246 160 6 83 322 136 374 2.89e-29 119
MS.gene68327.t1 AT5G20590 31.818 264 147 11 75 321 131 378 5.64e-28 114
MS.gene68327.t1 AT5G01620 26.216 370 236 13 77 431 102 449 2.02e-27 114
MS.gene68327.t1 AT5G01620 26.216 370 236 13 77 431 102 449 2.02e-27 114
MS.gene68327.t1 AT5G01620 26.216 370 236 13 77 431 110 457 2.05e-27 114
MS.gene68327.t1 AT5G58600 30.798 263 143 8 68 319 55 289 2.15e-26 108
MS.gene68327.t1 AT5G06230 35.821 201 105 8 83 262 60 257 7.38e-25 104
MS.gene68327.t1 AT2G31110 33.152 184 115 4 72 254 31 207 1.89e-24 102
MS.gene68327.t1 AT2G40160 31.771 192 121 4 26 214 22 206 1.75e-23 100
MS.gene68327.t1 AT2G30900 31.925 213 132 5 82 292 40 241 9.66e-23 97.8
MS.gene68327.t1 AT5G64470 27.138 269 176 10 85 341 55 315 3.67e-20 91.3
MS.gene68327.t1 AT5G64470 27.239 268 175 10 85 340 55 314 4.25e-20 90.9
MS.gene68327.t1 AT3G14850 30.142 282 159 11 156 431 2 251 3.68e-19 87.0
MS.gene68327.t1 AT1G78710 31.667 180 114 4 76 254 22 193 1.74e-18 85.9
MS.gene68327.t1 AT2G34070 28.417 278 159 15 156 427 2 245 4.86e-16 78.2
MS.gene68327.t1 AT2G31110 29.279 222 115 14 222 427 15 210 4.63e-11 62.8

Find 97 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCTCAATTATACTATTA+AGG 0.109514 5.2:+25637350 None:intergenic
GTGCCACTTACGAAATTTAT+TGG 0.120096 5.2:+25637666 None:intergenic
CTGATTTAGGTTATCTTTAT+TGG 0.135262 5.2:-25637867 MS.gene68327:CDS
TATGAGAGTGATTTAATTCT+TGG 0.184685 5.2:-25637448 MS.gene68327:CDS
GTCCTCAAAGCCTTTCTTAA+TGG 0.190045 5.2:+25637376 None:intergenic
TGCAAAGAAGTGACTCTAAT+TGG 0.285207 5.2:+25637737 None:intergenic
ACAAATGGGAGACCTGATTT+AGG 0.292037 5.2:-25637880 MS.gene68327:CDS
TCTCTCAATTATACTATTAA+GGG 0.345275 5.2:+25637351 None:intergenic
TCAACACCATGCACTAGAAA+TGG 0.356439 5.2:+25637604 None:intergenic
GAATGGATGGTGAGATTAGA+AGG 0.357080 5.2:-25637195 MS.gene68327:CDS
AATCTGAGTATGATTAAAAT+CGG 0.358474 5.2:+25637411 None:intergenic
TTGTAGAGATGATGAAGAAA+TGG 0.363410 5.2:-25636949 MS.gene68327:CDS
AGAAAAGTTGCTAAAGGAAA+TGG 0.366030 5.2:-25637331 MS.gene68327:CDS
TATAAACAAAGAAGGGATAT+AGG 0.366305 5.2:+25638372 None:intergenic
GTGACTATTCAAATGGAGAT+TGG 0.369081 5.2:-25637975 MS.gene68327:CDS
GGAGAAGATAGTTGGAGGAA+TGG 0.393313 5.2:-25637212 MS.gene68327:CDS
ATGTGGTGACATTGTATATA+GGG 0.397785 5.2:+25637935 None:intergenic
ATTTATTGGACTTGCCATCT+TGG 0.403569 5.2:+25637680 None:intergenic
AGAAAAGAGGAGAAGATAGT+TGG 0.414140 5.2:-25637220 MS.gene68327:CDS
CAACACCATGCACTAGAAAT+GGG 0.415330 5.2:+25637605 None:intergenic
CCATCTTGGTAAACAAGGTT+AGG 0.420926 5.2:+25637694 None:intergenic
TTCCATTAAGAAAGGCTTTG+AGG 0.431515 5.2:-25637378 MS.gene68327:CDS
TTTGGAACTCCATTTGCAAA+TGG 0.431908 5.2:+25637037 None:intergenic
TCACTCTCATAGTAAATTGA+AGG 0.436067 5.2:+25637460 None:intergenic
ACTCCATTTGCAAATGGAAA+AGG 0.436668 5.2:+25637043 None:intergenic
AGTGAGAGATGCATCACAAA+TGG 0.437580 5.2:-25637895 MS.gene68327:CDS
AAGAGTAAGTAGATGAATAG+TGG 0.440562 5.2:+25638280 None:intergenic
GGCAAGGGATATTGATCAAA+TGG 0.443552 5.2:-25637521 MS.gene68327:CDS
CATGTGGTGACATTGTATAT+AGG 0.444034 5.2:+25637934 None:intergenic
CAATAAAGATAACCTAAATC+AGG 0.445212 5.2:+25637868 None:intergenic
ACAAAAGCTAATAAATTTGA+AGG 0.447815 5.2:-25637142 MS.gene68327:CDS
GTTTGTTCTTAATGAGATTG+AGG 0.450023 5.2:+25637794 None:intergenic
TGTAGAGATGATGAAGAAAT+GGG 0.451545 5.2:-25636948 MS.gene68327:CDS
CTTGGTAGATTACACTCACT+TGG 0.451639 5.2:+25637835 None:intergenic
TGACTATTCAAATGGAGATT+GGG 0.453167 5.2:-25637974 MS.gene68327:CDS
TGTGTTCATTGGTGCTTGCC+AGG 0.456446 5.2:-25636998 MS.gene68327:CDS
ATTTGAAGGTATTAGAATTG+AGG 0.457268 5.2:-25637128 MS.gene68327:CDS
TACAAAACGATTGTGTTCAT+TGG 0.458956 5.2:-25637009 MS.gene68327:CDS
TAGGTTATCTTTATTGGAGA+TGG 0.460710 5.2:-25637861 MS.gene68327:CDS
TAAGGACCTGGATGACCATC+TGG 0.461971 5.2:+25637073 None:intergenic
AAATCATGTGACTATTCAAA+TGG 0.471379 5.2:-25637982 MS.gene68327:CDS
TTGAGACCAGATGGTCATCC+AGG 0.473377 5.2:-25637079 MS.gene68327:CDS
AAGGATTGAGATAGAAGAAG+TGG 0.473823 5.2:-25637176 MS.gene68327:CDS
TGGTCCCCATTTCTAGTGCA+TGG 0.482297 5.2:-25637610 MS.gene68327:CDS
TAGTTGGTCCAAAAGGAGTA+AGG 0.483038 5.2:+25638334 None:intergenic
GACTAAGCCTTATAGAAAAG+AGG 0.487707 5.2:-25637233 MS.gene68327:CDS
AGAAGCTTAGTTGGTCCAAA+AGG 0.489769 5.2:+25638327 None:intergenic
CCTAACCTTGTTTACCAAGA+TGG 0.495701 5.2:-25637694 MS.gene68327:CDS
AATGGAAAAGGATTCATGTA+AGG 0.496598 5.2:+25637055 None:intergenic
TGCCAGGACCTATAGATACT+TGG 0.502047 5.2:-25636982 MS.gene68327:CDS
GGATGAAGTATAAACAAAGA+AGG 0.502496 5.2:+25638364 None:intergenic
GGAGGAAAGATTGTCATTGA+AGG 0.503097 5.2:+25638437 None:intergenic
GGTGTTGGATGTAACTAAAC+TGG 0.504154 5.2:-25637107 MS.gene68327:CDS
GTAAGTAGATGAATAGTGGT+AGG 0.506588 5.2:+25638284 None:intergenic
GTAGAATGAAGAAGCTTAGT+TGG 0.508993 5.2:+25638318 None:intergenic
TTGTTGGGGATTCTTTGGCT+AGG 0.510878 5.2:-25637762 MS.gene68327:CDS
CAAAAGGAGTAAGGGACAAA+AGG 0.511314 5.2:+25638343 None:intergenic
ATCTGAGTATGATTAAAATC+GGG 0.514293 5.2:+25637412 None:intergenic
ATTGAGAGAAAAGTTGCTAA+AGG 0.514738 5.2:-25637337 MS.gene68327:CDS
AAAGAGGAGAAGATAGTTGG+AGG 0.516930 5.2:-25637217 MS.gene68327:CDS
AAAGTGCTTGGTTCAAACCT+TGG 0.519498 5.2:+25637817 None:intergenic
GAAGGTGATTGGGATAAGGC+TGG 0.521359 5.2:-25637268 MS.gene68327:CDS
GGTGTTGAAAGATCAAATGA+AGG 0.522081 5.2:-25637589 MS.gene68327:CDS
GAGGAAAGATTGTCATTGAA+GGG 0.526115 5.2:+25638438 None:intergenic
TGGGGACCAATACAAAGAAA+AGG 0.526383 5.2:+25637624 None:intergenic
GTGAGAGATGCATCACAAAT+GGG 0.526674 5.2:-25637894 MS.gene68327:CDS
GATGAAGTATAAACAAAGAA+GGG 0.527867 5.2:+25638365 None:intergenic
AGTCCAATAAATTTCGTAAG+TGG 0.528019 5.2:-25637669 MS.gene68327:CDS
TTCCAAGTATCTATAGGTCC+TGG 0.536833 5.2:+25636980 None:intergenic
ATACCTACAAAAGAAGTTGA+AGG 0.538773 5.2:+25638252 None:intergenic
GGATCTTGTCAATGAGAAGT+GGG 0.539423 5.2:-25637542 MS.gene68327:CDS
ATCTCATTCCAAGTATCTAT+AGG 0.540214 5.2:+25636974 None:intergenic
AAGATAGTTGGAGGAATGGA+TGG 0.542345 5.2:-25637208 MS.gene68327:CDS
CAATCACCTTCAAAATGAGC+AGG 0.550833 5.2:+25637280 None:intergenic
ATGAGATTGAGGAAAGTGCT+TGG 0.551052 5.2:+25637805 None:intergenic
TTCTTTGATTGTGCCACATG+TGG 0.561553 5.2:+25637918 None:intergenic
AGGTATTAGAATTGAGGTGT+TGG 0.565513 5.2:-25637122 MS.gene68327:CDS
TGGATCTTGTCAATGAGAAG+TGG 0.565608 5.2:-25637543 MS.gene68327:CDS
GAAAAGGTAGCATTATGAGA+AGG 0.570303 5.2:+25637640 None:intergenic
AGGAAAGATTGTCATTGAAG+GGG 0.571923 5.2:+25638439 None:intergenic
TGAATAGTGGTAGGTGGCAA+TGG 0.576540 5.2:+25638293 None:intergenic
TTGTCAATGAGAAGTGGGCA+AGG 0.584004 5.2:-25637537 MS.gene68327:CDS
ACTTGCCATCTTGGTAAACA+AGG 0.587524 5.2:+25637689 None:intergenic
GAAAGGGTTTGAAAATGGGA+AGG 0.593746 5.2:-25638467 None:intergenic
AAAGGATTCATGTAAGGACC+TGG 0.594011 5.2:+25637061 None:intergenic
AAAGGGTTTGAAAATGGGAA+GGG 0.612498 5.2:-25638466 None:intergenic
AGTAGATGAATAGTGGTAGG+TGG 0.614813 5.2:+25638287 None:intergenic
AGTTGGTCCAAAAGGAGTAA+GGG 0.618383 5.2:+25638335 None:intergenic
CTGGCATTATTGAGACCAGA+TGG 0.624189 5.2:-25637088 MS.gene68327:CDS
ATATACAATGTCACCACATG+TGG 0.627135 5.2:-25637931 MS.gene68327:CDS
GTGTTGAAAGATCAAATGAA+GGG 0.634222 5.2:-25637588 MS.gene68327:CDS
CAAGTGAGTGTAATCTACCA+AGG 0.650297 5.2:-25637834 MS.gene68327:CDS
TGTCAATGAGAAGTGGGCAA+GGG 0.656260 5.2:-25637536 MS.gene68327:CDS
CCTACAAAAGAAGTTGAAGG+AGG 0.659746 5.2:+25638255 None:intergenic
AACACCATGCACTAGAAATG+GGG 0.662494 5.2:+25637606 None:intergenic
GATTGGGTTAGTGACATGAG+AGG 0.690038 5.2:-25637958 MS.gene68327:CDS
ATAGTGGTAGGTGGCAATGG+TGG 0.693322 5.2:+25638296 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTATAATACAATGTTTT+TGG - chr5.2:25637833-25637852 MS.gene68327:CDS 10.0%
!! ATGAAAAAATTTCTTATTGA+AGG + chr5.2:25636983-25637002 None:intergenic 15.0%
!!! TATGTAATATATTTTTTGCA+GGG - chr5.2:25637367-25637386 MS.gene68327:CDS 15.0%
!!! TTATGTAATATATTTTTTGC+AGG - chr5.2:25637366-25637385 MS.gene68327:CDS 15.0%
!! AAAAAATTTCTTATTGAAGG+AGG + chr5.2:25636980-25636999 None:intergenic 20.0%
!! AATCTGAGTATGATTAAAAT+CGG + chr5.2:25637988-25638007 None:intergenic 20.0%
!! ACAAAAGCTAATAAATTTGA+AGG - chr5.2:25638254-25638273 MS.gene68327:intron 20.0%
!! TCTCTCAATTATACTATTAA+GGG + chr5.2:25638048-25638067 None:intergenic 20.0%
!! TTCTCTCAATTATACTATTA+AGG + chr5.2:25638049-25638068 None:intergenic 20.0%
!!! AAATTTATTAGCTTTTGTCT+TGG + chr5.2:25638252-25638271 None:intergenic 20.0%
!!! TTTTTATGTTCCATTAAGAA+AGG - chr5.2:25638010-25638029 MS.gene68327:CDS 20.0%
! AAATCATGTGACTATTCAAA+TGG - chr5.2:25637414-25637433 MS.gene68327:CDS 25.0%
! ATCTGAGTATGATTAAAATC+GGG + chr5.2:25637987-25638006 None:intergenic 25.0%
! CAATAAAGATAACCTAAATC+AGG + chr5.2:25637531-25637550 None:intergenic 25.0%
! CCTTAATAATAACAAATTGC+TGG + chr5.2:25637326-25637345 None:intergenic 25.0%
! GATGAAGTATAAACAAAGAA+GGG + chr5.2:25637034-25637053 None:intergenic 25.0%
! TATAAACAAAGAAGGGATAT+AGG + chr5.2:25637027-25637046 None:intergenic 25.0%
! TATGAGAGTGATTTAATTCT+TGG - chr5.2:25637948-25637967 MS.gene68327:CDS 25.0%
! TGTTATTATTAAGGACTTCT+TGG - chr5.2:25637332-25637351 MS.gene68327:CDS 25.0%
!! AACAAACATATAGCTTTTGT+TGG - chr5.2:25637618-25637637 MS.gene68327:CDS 25.0%
!! ACAAACATATAGCTTTTGTT+GGG - chr5.2:25637619-25637638 MS.gene68327:CDS 25.0%
!! CAATCGTTTTGTACATATTT+TGG + chr5.2:25638380-25638399 None:intergenic 25.0%
!! CTGATTTAGGTTATCTTTAT+TGG - chr5.2:25637529-25637548 MS.gene68327:CDS 25.0%
!!! ACATGATTGTTTTATCAACT+GGG - chr5.2:25637898-25637917 MS.gene68327:CDS 25.0%
!!! ATTTGAAGGTATTAGAATTG+AGG - chr5.2:25638268-25638287 MS.gene68327:CDS 25.0%
!!! CCAGCAATTTGTTATTATTA+AGG - chr5.2:25637323-25637342 MS.gene68327:CDS 25.0%
AAGAGTAAGTAGATGAATAG+TGG + chr5.2:25637119-25637138 None:intergenic 30.0%
AATGGAAAAGGATTCATGTA+AGG + chr5.2:25638344-25638363 None:intergenic 30.0%
AGAAAAGTTGCTAAAGGAAA+TGG - chr5.2:25638065-25638084 MS.gene68327:intron 30.0%
AGTCCAATAAATTTCGTAAG+TGG - chr5.2:25637727-25637746 MS.gene68327:CDS 30.0%
ATACCTACAAAAGAAGTTGA+AGG + chr5.2:25637147-25637166 None:intergenic 30.0%
ATCTCATTCCAAGTATCTAT+AGG + chr5.2:25638425-25638444 None:intergenic 30.0%
ATGTGGTGACATTGTATATA+GGG + chr5.2:25637464-25637483 None:intergenic 30.0%
ATTGAGAGAAAAGTTGCTAA+AGG - chr5.2:25638059-25638078 MS.gene68327:intron 30.0%
GGATGAAGTATAAACAAAGA+AGG + chr5.2:25637035-25637054 None:intergenic 30.0%
GTGTTGAAAGATCAAATGAA+GGG - chr5.2:25637808-25637827 MS.gene68327:CDS 30.0%
GTTTGTTCTTAATGAGATTG+AGG + chr5.2:25637605-25637624 None:intergenic 30.0%
TACAAAACGATTGTGTTCAT+TGG - chr5.2:25638387-25638406 MS.gene68327:CDS 30.0%
TAGGTTATCTTTATTGGAGA+TGG - chr5.2:25637535-25637554 MS.gene68327:CDS 30.0%
TCACTCTCATAGTAAATTGA+AGG + chr5.2:25637939-25637958 None:intergenic 30.0%
TGACTATTCAAATGGAGATT+GGG - chr5.2:25637422-25637441 MS.gene68327:CDS 30.0%
TGTAGAGATGATGAAGAAAT+GGG - chr5.2:25638448-25638467 MS.gene68327:CDS 30.0%
TTGTAGAGATGATGAAGAAA+TGG - chr5.2:25638447-25638466 MS.gene68327:CDS 30.0%
! AATCCTTTTCCATTTGCAAA+TGG - chr5.2:25638350-25638369 MS.gene68327:CDS 30.0%
! ATCTTCTCCTCTTTTCTATA+AGG + chr5.2:25638173-25638192 None:intergenic 30.0%
! ATGCTACCTTTTCTTTGTAT+TGG - chr5.2:25637766-25637785 MS.gene68327:CDS 30.0%
! CAAACATATAGCTTTTGTTG+GGG - chr5.2:25637620-25637639 MS.gene68327:CDS 30.0%
!! GACATGATTGTTTTATCAAC+TGG - chr5.2:25637897-25637916 MS.gene68327:CDS 30.0%
AAGGATTGAGATAGAAGAAG+TGG - chr5.2:25638220-25638239 MS.gene68327:intron 35.0%
ACTCCATTTGCAAATGGAAA+AGG + chr5.2:25638356-25638375 None:intergenic 35.0%
AGAAAAGAGGAGAAGATAGT+TGG - chr5.2:25638176-25638195 MS.gene68327:intron 35.0%
AGGAAAGATTGTCATTGAAG+GGG + chr5.2:25636960-25636979 None:intergenic 35.0%
AGGTATTAGAATTGAGGTGT+TGG - chr5.2:25638274-25638293 MS.gene68327:CDS 35.0%
ATATACAATGTCACCACATG+TGG - chr5.2:25637465-25637484 MS.gene68327:CDS 35.0%
ATTTATTGGACTTGCCATCT+TGG + chr5.2:25637719-25637738 None:intergenic 35.0%
CATGTGGTGACATTGTATAT+AGG + chr5.2:25637465-25637484 None:intergenic 35.0%
GAAAAGGTAGCATTATGAGA+AGG + chr5.2:25637759-25637778 None:intergenic 35.0%
GAGGAAAGATTGTCATTGAA+GGG + chr5.2:25636961-25636980 None:intergenic 35.0%
GTAAGTAGATGAATAGTGGT+AGG + chr5.2:25637115-25637134 None:intergenic 35.0%
GTAGAATGAAGAAGCTTAGT+TGG + chr5.2:25637081-25637100 None:intergenic 35.0%
GTGACTATTCAAATGGAGAT+TGG - chr5.2:25637421-25637440 MS.gene68327:CDS 35.0%
TGCAAAGAAGTGACTCTAAT+TGG + chr5.2:25637662-25637681 None:intergenic 35.0%
TTCCATTAAGAAAGGCTTTG+AGG - chr5.2:25638018-25638037 MS.gene68327:intron 35.0%
! GACTAAGCCTTATAGAAAAG+AGG - chr5.2:25638163-25638182 MS.gene68327:intron 35.0%
! GGTGTTGAAAGATCAAATGA+AGG - chr5.2:25637807-25637826 MS.gene68327:CDS 35.0%
! TTTGGAACTCCATTTGCAAA+TGG + chr5.2:25638362-25638381 None:intergenic 35.0%
! TTTTCACCTGCTCATTTTGA+AGG - chr5.2:25638110-25638129 MS.gene68327:intron 35.0%
!! GTGCCACTTACGAAATTTAT+TGG + chr5.2:25637733-25637752 None:intergenic 35.0%
!! TGCTCATTTTGAAGGTGATT+GGG - chr5.2:25638118-25638137 MS.gene68327:intron 35.0%
!! TTGTTTTATCAACTGGGCAT+TGG - chr5.2:25637904-25637923 MS.gene68327:CDS 35.0%
!! TTTTGAAGGTGATTGGGATA+AGG - chr5.2:25638124-25638143 MS.gene68327:intron 35.0%
!!! ATTTTTTGCAGGGAAGAACA+AGG - chr5.2:25637377-25637396 MS.gene68327:CDS 35.0%
AAAGAGGAGAAGATAGTTGG+AGG - chr5.2:25638179-25638198 MS.gene68327:intron 40.0%
AAAGGATTCATGTAAGGACC+TGG + chr5.2:25638338-25638357 None:intergenic 40.0%
AACACCATGCACTAGAAATG+GGG + chr5.2:25637793-25637812 None:intergenic 40.0%
AAGATAGTTGGAGGAATGGA+TGG - chr5.2:25638188-25638207 MS.gene68327:intron 40.0%
ACAAATGGGAGACCTGATTT+AGG - chr5.2:25637516-25637535 MS.gene68327:CDS 40.0%
ACTTGCCATCTTGGTAAACA+AGG + chr5.2:25637710-25637729 None:intergenic 40.0%
AGAAGCTTAGTTGGTCCAAA+AGG + chr5.2:25637072-25637091 None:intergenic 40.0%
AGTAGATGAATAGTGGTAGG+TGG + chr5.2:25637112-25637131 None:intergenic 40.0%
AGTGAGAGATGCATCACAAA+TGG - chr5.2:25637501-25637520 MS.gene68327:CDS 40.0%
AGTTGGTCCAAAAGGAGTAA+GGG + chr5.2:25637064-25637083 None:intergenic 40.0%
CAAAAGGAGTAAGGGACAAA+AGG + chr5.2:25637056-25637075 None:intergenic 40.0%
CAACACCATGCACTAGAAAT+GGG + chr5.2:25637794-25637813 None:intergenic 40.0%
CAAGTGAGTGTAATCTACCA+AGG - chr5.2:25637562-25637581 MS.gene68327:CDS 40.0%
CAATCACCTTCAAAATGAGC+AGG + chr5.2:25638119-25638138 None:intergenic 40.0%
CCATCTTGGTAAACAAGGTT+AGG + chr5.2:25637705-25637724 None:intergenic 40.0%
CCTACAAAAGAAGTTGAAGG+AGG + chr5.2:25637144-25637163 None:intergenic 40.0%
CTTGGTAGATTACACTCACT+TGG + chr5.2:25637564-25637583 None:intergenic 40.0%
GAATGGATGGTGAGATTAGA+AGG - chr5.2:25638201-25638220 MS.gene68327:intron 40.0%
GGAGGAAAGATTGTCATTGA+AGG + chr5.2:25636962-25636981 None:intergenic 40.0%
GGATCTTGTCAATGAGAAGT+GGG - chr5.2:25637854-25637873 MS.gene68327:CDS 40.0%
GGCAAGGGATATTGATCAAA+TGG - chr5.2:25637875-25637894 MS.gene68327:CDS 40.0%
GTCCTCAAAGCCTTTCTTAA+TGG + chr5.2:25638023-25638042 None:intergenic 40.0%
GTGAGAGATGCATCACAAAT+GGG - chr5.2:25637502-25637521 MS.gene68327:CDS 40.0%
TAGTTGGTCCAAAAGGAGTA+AGG + chr5.2:25637065-25637084 None:intergenic 40.0%
TCAACACCATGCACTAGAAA+TGG + chr5.2:25637795-25637814 None:intergenic 40.0%
TGGATCTTGTCAATGAGAAG+TGG - chr5.2:25637853-25637872 MS.gene68327:CDS 40.0%
TGGGGACCAATACAAAGAAA+AGG + chr5.2:25637775-25637794 None:intergenic 40.0%
TTCCAAGTATCTATAGGTCC+TGG + chr5.2:25638419-25638438 None:intergenic 40.0%
TTCTTTGATTGTGCCACATG+TGG + chr5.2:25637481-25637500 None:intergenic 40.0%
! AAAGTGCTTGGTTCAAACCT+TGG + chr5.2:25637582-25637601 None:intergenic 40.0%
! AGCTTTTGTTGGGGATTCTT+TGG - chr5.2:25637629-25637648 MS.gene68327:CDS 40.0%
! CCTAACCTTGTTTACCAAGA+TGG - chr5.2:25637702-25637721 MS.gene68327:CDS 40.0%
! CCTCCTTCAACTTCTTTTGT+AGG - chr5.2:25637141-25637160 MS.gene68327:CDS 40.0%
! CTTTTGTCCCTTACTCCTTT+TGG - chr5.2:25637054-25637073 MS.gene68327:CDS 40.0%
! GGTGTTGGATGTAACTAAAC+TGG - chr5.2:25638289-25638308 MS.gene68327:CDS 40.0%
!! ATGAGATTGAGGAAAGTGCT+TGG + chr5.2:25637594-25637613 None:intergenic 40.0%
!! CTGCTCATTTTGAAGGTGAT+TGG - chr5.2:25638117-25638136 MS.gene68327:intron 40.0%
CTGGCATTATTGAGACCAGA+TGG - chr5.2:25638308-25638327 MS.gene68327:CDS 45.0%
GGAGAAGATAGTTGGAGGAA+TGG - chr5.2:25638184-25638203 MS.gene68327:intron 45.0%
TGAATAGTGGTAGGTGGCAA+TGG + chr5.2:25637106-25637125 None:intergenic 45.0%
TGCCAGGACCTATAGATACT+TGG - chr5.2:25638414-25638433 MS.gene68327:CDS 45.0%
TGTCAATGAGAAGTGGGCAA+GGG - chr5.2:25637860-25637879 MS.gene68327:CDS 45.0%
TTGTCAATGAGAAGTGGGCA+AGG - chr5.2:25637859-25637878 MS.gene68327:CDS 45.0%
TTGTTGGGGATTCTTTGGCT+AGG - chr5.2:25637634-25637653 MS.gene68327:CDS 45.0%
!! GATTGGGTTAGTGACATGAG+AGG - chr5.2:25637438-25637457 MS.gene68327:CDS 45.0%
ATAGTGGTAGGTGGCAATGG+TGG + chr5.2:25637103-25637122 None:intergenic 50.0%
GAAGGTGATTGGGATAAGGC+TGG - chr5.2:25638128-25638147 MS.gene68327:intron 50.0%
TAAGGACCTGGATGACCATC+TGG + chr5.2:25638326-25638345 None:intergenic 50.0%
TTGAGACCAGATGGTCATCC+AGG - chr5.2:25638317-25638336 MS.gene68327:CDS 50.0%
! TGTGTTCATTGGTGCTTGCC+AGG - chr5.2:25638398-25638417 MS.gene68327:CDS 50.0%
!! TGGTCCCCATTTCTAGTGCA+TGG - chr5.2:25637786-25637805 MS.gene68327:CDS 50.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 25636943 25638475 25636943 ID=MS.gene68327
chr5.2 mRNA 25636943 25638475 25636943 ID=MS.gene68327.t1;Parent=MS.gene68327
chr5.2 exon 25638256 25638475 25638256 ID=MS.gene68327.t1.exon1;Parent=MS.gene68327.t1
chr5.2 CDS 25638256 25638475 25638256 ID=cds.MS.gene68327.t1;Parent=MS.gene68327.t1
chr5.2 exon 25636943 25638030 25636943 ID=MS.gene68327.t1.exon2;Parent=MS.gene68327.t1
chr5.2 CDS 25636943 25638030 25636943 ID=cds.MS.gene68327.t1;Parent=MS.gene68327.t1
Gene Sequence

>MS.gene68327

ATGGGAAGGGCAAACCCCTTCAATGACAATCTTTCCTCCTTCAATAAGAAATTTTTTCATTTGACTCTTTATTCATTGCTTCCTATATCCCTTCTTTGTTTATACTTCATCCTTTTGTCCCTTACTCCTTTTGGACCAACTAAGCTTCTTCATTCTACCACCATTGCCACCTACCACTATTCATCTACTTACTCTTCTCCTCCTTCAACTTCTTTTGTAGGTATATGCATGCTGCATAATTAACTTCTAGTTGAGATTCACATGAACTCTAACTAGCAATTTGAGATCATGCTAGTTGAGATTCACATGTACTCTAACTTCTATTCATCTACTTTAAAAAAAAAAAAAATCATGCTAGTTGAGATTCACATGAAGTCTAACCAGCAATTTGTTATTATTAAGGACTTCTTGGTTTACTAATTTTTATGTAATATATTTTTTGCAGGGAAGAACAAGGTTTATGATTATGAGAAATCATGTGACTATTCAAATGGAGATTGGGTTAGTGACATGAGAGGCCCTATATACAATGTCACCACATGTGGCACAATCAAAGAAAGTGAGAGATGCATCACAAATGGGAGACCTGATTTAGGTTATCTTTATTGGAGATGGAAGCCAAGTGAGTGTAATCTACCAAGGTTTGAACCAAGCACTTTCCTCAATCTCATTAAGAACAAACATATAGCTTTTGTTGGGGATTCTTTGGCTAGGAACCAATTAGAGTCACTTCTTTGCATGTTATTGACTACTTCAACACCTAACCTTGTTTACCAAGATGGCAAGTCCAATAAATTTCGTAAGTGGCACTTTCCTTCTCATAATGCTACCTTTTCTTTGTATTGGTCCCCATTTCTAGTGCATGGTGTTGAAAGATCAAATGAAGGGAATTATTATAATACAATGTTTTTGGATCTTGTCAATGAGAAGTGGGCAAGGGATATTGATCAAATGGACATGATTGTTTTATCAACTGGGCATTGGTTTTTGCTTCCTTCAATTTACTATGAGAGTGATTTAATTCTTGGTAGCTTGAATTGTCCCGATTTTAATCATACTCAGATTGATTTTTATGTTCCATTAAGAAAGGCTTTGAGGACTACCCTTAATAGTATAATTGAGAGAAAAGTTGCTAAAGGAAATGGAATTGATGTGATTGTGAAAACATTTTCACCTGCTCATTTTGAAGGTGATTGGGATAAGGCTGGTACTTGTTCAAAGACTAAGCCTTATAGAAAAGAGGAGAAGATAGTTGGAGGAATGGATGGTGAGATTAGAAGGATTGAGATAGAAGAAGTGGAAAGTGCCAAGACAAAAGCTAATAAATTTGAAGGTATTAGAATTGAGGTGTTGGATGTAACTAAACTGGCATTATTGAGACCAGATGGTCATCCAGGTCCTTACATGAATCCTTTTCCATTTGCAAATGGAGTTCCAAAATATGTACAAAACGATTGTGTTCATTGGTGCTTGCCAGGACCTATAGATACTTGGAATGAGATTCTTGTAGAGATGATGAAGAAATGGGAGTAA
Protein sequence

>MS.gene68327.t1

MGRANPFNDNLSSFNKKFFHLTLYSLLPISLLCLYFILLSLTPFGPTKLLHSTTIATYHYSSTYSSPPSTSFVGKNKVYDYEKSCDYSNGDWVSDMRGPIYNVTTCGTIKESERCITNGRPDLGYLYWRWKPSECNLPRFEPSTFLNLIKNKHIAFVGDSLARNQLESLLCMLLTTSTPNLVYQDGKSNKFRKWHFPSHNATFSLYWSPFLVHGVERSNEGNYYNTMFLDLVNEKWARDIDQMDMIVLSTGHWFLLPSIYYESDLILGSLNCPDFNHTQIDFYVPLRKALRTTLNSIIERKVAKGNGIDVIVKTFSPAHFEGDWDKAGTCSKTKPYRKEEKIVGGMDGEIRRIEIEEVESAKTKANKFEGIRIEVLDVTKLALLRPDGHPGPYMNPFPFANGVPKYVQNDCVHWCLPGPIDTWNEILVEMMKKWE