AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68334

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68334.t1 MTR_5g035070 96.818 440 14 0 1 440 1 440 0.0 893
MS.gene68334.t1 MTR_5g035090 68.950 438 132 4 3 440 1 434 0.0 633
MS.gene68334.t1 MTR_5g035150 65.517 435 148 2 5 437 1 435 0.0 600
MS.gene68334.t1 MTR_5g035080 62.779 403 132 5 38 437 37 424 0.0 524
MS.gene68334.t1 MTR_3g109840 57.500 440 162 9 4 440 5 422 2.35e-180 510
MS.gene68334.t1 MTR_8g104970 51.489 470 178 7 18 441 8 473 3.43e-172 491
MS.gene68334.t1 MTR_7g033230 58.221 371 145 5 68 435 248 611 3.91e-160 466
MS.gene68334.t1 MTR_7g033230 58.221 371 145 5 68 435 318 681 3.00e-159 466
MS.gene68334.t1 MTR_5g035140 62.313 268 94 4 174 438 7 270 1.75e-120 352
MS.gene68334.t1 MTR_6g016880 41.404 413 224 10 30 434 18 420 1.48e-112 338
MS.gene68334.t1 MTR_8g099300 39.862 434 241 10 5 433 1 419 1.56e-107 325
MS.gene68334.t1 MTR_7g019120 39.059 425 236 10 17 434 13 421 2.12e-106 322
MS.gene68334.t1 MTR_8g027900 45.787 356 179 7 84 433 208 555 3.01e-106 326
MS.gene68334.t1 MTR_6g007777 36.481 466 247 12 8 435 5 459 2.40e-96 298
MS.gene68334.t1 MTR_6g016875 38.983 413 217 9 30 434 24 409 1.14e-91 284
MS.gene68334.t1 MTR_2g016060 40.274 365 192 14 84 435 51 402 1.18e-85 268
MS.gene68334.t1 MTR_1g076690 36.438 365 208 11 87 438 223 576 2.34e-70 233
MS.gene68334.t1 MTR_6g061710 33.741 409 240 10 43 435 123 516 1.05e-68 227
MS.gene68334.t1 MTR_7g094750 34.072 361 209 13 87 435 91 434 1.41e-55 191
MS.gene68334.t1 MTR_0136s0010 35.792 366 195 14 87 435 170 512 1.45e-53 187
MS.gene68334.t1 MTR_7g093450 35.933 359 191 13 87 429 248 583 1.71e-53 188
MS.gene68334.t1 MTR_5g035120 52.874 174 71 3 5 177 2 165 3.63e-53 176
MS.gene68334.t1 MTR_2g022560 34.066 364 210 14 86 437 96 441 3.46e-49 174
MS.gene68334.t1 MTR_4g126950 31.458 391 231 15 57 433 79 446 4.22e-48 171
MS.gene68334.t1 MTR_4g121560 29.397 398 238 15 57 433 78 453 8.71e-47 167
MS.gene68334.t1 MTR_5g059400 31.675 382 205 15 86 440 174 526 2.69e-46 167
MS.gene68334.t1 MTR_1g116130 31.675 382 205 15 86 440 174 526 2.69e-46 167
MS.gene68334.t1 MTR_8g069790 30.245 367 219 12 87 438 178 522 1.28e-45 166
MS.gene68334.t1 MTR_5g033800 28.272 382 226 13 78 439 137 490 5.65e-45 163
MS.gene68334.t1 MTR_4g016940 29.834 362 229 11 82 433 81 427 5.29e-44 159
MS.gene68334.t1 MTR_8g093530 29.494 356 209 11 87 431 90 414 1.64e-43 158
MS.gene68334.t1 MTR_5g071350 30.328 366 227 11 75 429 47 395 3.95e-43 156
MS.gene68334.t1 MTR_7g109550 31.421 366 207 13 87 438 137 472 7.39e-43 157
MS.gene68334.t1 MTR_7g070060 29.539 369 221 15 80 429 57 405 7.73e-42 153
MS.gene68334.t1 MTR_1g058380 27.739 429 253 16 29 438 10 400 2.96e-41 151
MS.gene68334.t1 MTR_7g068920 31.492 362 223 13 82 433 75 421 7.48e-41 150
MS.gene68334.t1 MTR_7g094750 35.772 246 141 6 87 323 91 328 7.88e-41 148
MS.gene68334.t1 MTR_4g014440 30.110 362 214 14 87 429 62 403 1.67e-40 149
MS.gene68334.t1 MTR_3g070900 30.137 365 208 15 87 429 106 445 2.05e-40 150
MS.gene68334.t1 MTR_3g464570 30.286 350 219 12 86 429 42 372 1.26e-39 146
MS.gene68334.t1 MTR_8g463790 28.962 366 218 12 87 435 71 411 1.61e-39 147
MS.gene68334.t1 MTR_3g035830 30.058 346 209 10 86 429 30 344 2.24e-39 145
MS.gene68334.t1 MTR_3g021090 31.054 351 199 14 86 429 30 344 6.02e-39 145
MS.gene68334.t1 MTR_3g021060 30.286 350 201 12 87 429 46 359 3.82e-38 142
MS.gene68334.t1 MTR_5g083260 27.610 431 268 14 19 430 16 421 5.42e-38 143
MS.gene68334.t1 MTR_1g050448 30.000 360 220 10 78 433 44 375 5.12e-37 139
MS.gene68334.t1 MTR_4g125060 28.351 388 210 13 75 430 117 468 1.63e-36 140
MS.gene68334.t1 MTR_2g091175 30.347 346 205 12 87 429 50 362 2.04e-36 137
MS.gene68334.t1 MTR_4g125060 28.158 380 208 12 80 430 164 507 2.63e-36 140
MS.gene68334.t1 MTR_4g125060 28.158 380 208 12 80 430 193 536 3.25e-36 140
MS.gene68334.t1 MTR_1g112210 28.767 365 193 15 87 429 59 378 4.24e-36 137
MS.gene68334.t1 MTR_8g093870 26.234 385 224 12 74 430 117 469 6.70e-36 138
MS.gene68334.t1 MTR_3g071650 27.635 427 265 17 27 430 22 427 7.79e-36 137
MS.gene68334.t1 MTR_7g019140 40.491 163 87 3 19 178 15 170 3.02e-35 129
MS.gene68334.t1 MTR_3g090120 27.553 421 251 17 30 433 76 459 3.12e-35 136
MS.gene68334.t1 MTR_3g049330 29.412 357 215 10 78 429 22 346 3.20e-35 134
MS.gene68334.t1 MTR_1g112210 28.729 362 184 15 87 429 59 365 6.15e-35 133
MS.gene68334.t1 MTR_4g088030 27.095 358 225 10 73 429 24 346 1.35e-34 132
MS.gene68334.t1 MTR_2g073500 30.199 351 199 14 87 429 29 341 1.71e-34 132
MS.gene68334.t1 MTR_6g016240 30.189 371 208 17 86 431 70 414 2.11e-34 133
MS.gene68334.t1 MTR_4g079700 27.494 411 237 13 29 433 8 363 2.21e-34 132
MS.gene68334.t1 MTR_4g087730 28.099 363 214 12 75 429 35 358 7.53e-34 130
MS.gene68334.t1 MTR_5g082880 28.415 366 216 15 87 429 165 507 2.05e-33 132
MS.gene68334.t1 MTR_2g073510 29.972 357 204 14 87 433 33 353 3.02e-33 128
MS.gene68334.t1 MTR_4g014430 27.749 382 220 15 76 433 97 446 2.64e-32 127
MS.gene68334.t1 MTR_2g015720 29.060 351 217 12 84 433 39 358 3.55e-32 125
MS.gene68334.t1 MTR_1g111010 29.944 354 197 16 87 433 32 341 3.07e-31 123
MS.gene68334.t1 MTR_5g034810 29.609 358 215 13 76 429 24 348 2.01e-30 120
MS.gene68334.t1 MTR_5g033800 29.615 260 151 8 78 322 137 379 2.30e-30 121
MS.gene68334.t1 MTR_4g092830 25.926 378 216 15 87 433 48 392 1.25e-27 114
MS.gene68334.t1 MTR_7g070060 33.862 189 110 5 80 259 57 239 1.79e-27 112
MS.gene68334.t1 MTR_4g102570 38.462 156 83 4 203 352 2 150 7.37e-26 103
MS.gene68334.t1 MTR_7g070060 27.508 309 186 14 139 429 15 303 3.10e-23 99.8
MS.gene68334.t1 MTR_7g117160 32.420 219 90 7 214 432 29 189 3.52e-22 94.0
MS.gene68334.t1 MTR_7g019130 35.938 128 75 3 205 328 1 125 5.14e-22 92.0
MS.gene68334.t1 MTR_3g464570 26.667 285 184 12 151 429 3 268 1.88e-18 85.5
MS.gene68334.t1 MTR_0929s0010 55.102 49 22 0 87 135 79 127 4.05e-15 72.0
MS.gene68334.t1 MTR_0929s0020 34.524 84 52 1 205 285 1 84 8.49e-13 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68334.t1 AT1G70230 57.518 419 161 6 20 437 13 415 4.47e-179 506
MS.gene68334.t1 AT1G01430 42.785 395 208 9 46 433 64 447 4.43e-106 322
MS.gene68334.t1 AT4G01080 45.380 368 178 12 73 431 75 428 8.89e-105 318
MS.gene68334.t1 AT5G15890 41.624 394 212 8 42 434 147 523 6.49e-104 319
MS.gene68334.t1 AT3G02440 43.503 354 183 7 87 434 135 477 9.42e-101 309
MS.gene68334.t1 AT3G02440 43.503 354 183 7 87 434 127 469 1.11e-100 309
MS.gene68334.t1 AT4G23790 39.277 415 240 8 27 433 17 427 1.64e-100 307
MS.gene68334.t1 AT5G15900 41.388 389 197 12 66 437 39 413 1.10e-97 300
MS.gene68334.t1 AT5G15900 41.388 389 197 12 66 437 58 432 3.09e-97 299
MS.gene68334.t1 AT3G28150 41.127 355 195 7 85 435 68 412 1.78e-95 293
MS.gene68334.t1 AT4G11090 41.161 379 209 9 62 433 54 425 1.87e-93 289
MS.gene68334.t1 AT4G25360 36.247 389 227 9 59 435 147 526 1.19e-74 243
MS.gene68334.t1 AT4G25360 36.247 389 227 9 59 435 147 526 1.19e-74 243
MS.gene68334.t1 AT5G51640 35.616 365 207 11 87 435 138 490 1.26e-66 221
MS.gene68334.t1 AT5G19160 33.702 362 221 9 87 439 99 450 1.02e-55 191
MS.gene68334.t1 AT3G12060 31.378 392 230 13 54 429 163 531 5.63e-51 181
MS.gene68334.t1 AT3G06080 34.807 362 212 12 87 438 103 450 6.06e-51 179
MS.gene68334.t1 AT5G06700 33.880 366 202 16 87 435 255 597 1.72e-49 178
MS.gene68334.t1 AT5G64020 31.250 368 212 13 86 430 56 405 9.20e-48 169
MS.gene68334.t1 AT1G48880 34.254 362 180 16 87 430 115 436 1.52e-47 169
MS.gene68334.t1 AT5G20590 32.961 358 202 13 87 429 141 475 7.00e-47 168
MS.gene68334.t1 AT2G30010 31.169 385 231 13 63 433 32 396 2.03e-44 160
MS.gene68334.t1 AT5G06230 32.231 363 209 14 84 429 18 360 3.26e-44 159
MS.gene68334.t1 AT5G49340 30.155 388 241 14 57 433 73 441 3.26e-44 160
MS.gene68334.t1 AT3G11570 31.977 344 191 13 103 429 98 415 4.03e-44 160
MS.gene68334.t1 AT5G06230 32.231 363 209 14 84 429 59 401 4.65e-44 159
MS.gene68334.t1 AT2G30010 31.169 385 231 13 63 433 75 439 4.97e-44 160
MS.gene68334.t1 AT3G62390 30.328 366 209 12 87 435 138 474 4.95e-43 158
MS.gene68334.t1 AT2G40160 28.404 426 251 18 30 429 22 419 2.71e-42 155
MS.gene68334.t1 AT2G40160 28.404 426 251 18 30 429 22 419 3.59e-42 155
MS.gene68334.t1 AT1G73140 31.978 369 208 17 78 429 59 401 1.06e-41 153
MS.gene68334.t1 AT2G14530 29.777 403 227 16 58 433 33 406 1.16e-41 152
MS.gene68334.t1 AT5G19160 36.475 244 140 6 87 322 99 335 1.47e-40 148
MS.gene68334.t1 AT1G60790 29.459 370 214 14 87 434 190 534 4.65e-40 150
MS.gene68334.t1 AT2G40320 28.969 359 222 13 86 430 80 419 1.51e-39 147
MS.gene68334.t1 AT5G58600 29.558 362 215 12 86 433 65 400 2.73e-39 146
MS.gene68334.t1 AT2G38320 28.775 351 233 10 87 429 59 400 6.61e-39 145
MS.gene68334.t1 AT2G37720 28.533 368 218 11 82 430 138 479 8.19e-39 146
MS.gene68334.t1 AT3G11030 29.050 358 222 13 87 430 106 445 1.18e-38 145
MS.gene68334.t1 AT5G01360 28.919 370 217 15 82 430 31 375 4.41e-38 142
MS.gene68334.t1 AT2G42570 30.966 352 204 13 87 433 47 364 5.16e-38 142
MS.gene68334.t1 AT3G55990 30.194 361 213 14 87 429 140 479 7.38e-38 144
MS.gene68334.t1 AT5G01360 28.571 371 217 15 82 430 87 431 1.43e-37 142
MS.gene68334.t1 AT3G14850 29.096 354 211 10 87 433 34 354 1.43e-37 140
MS.gene68334.t1 AT2G30900 29.226 349 211 10 87 429 43 361 8.53e-37 139
MS.gene68334.t1 AT2G40150 28.608 388 236 15 64 429 48 416 9.51e-37 139
MS.gene68334.t1 AT2G31110 29.191 346 210 12 87 429 45 358 2.18e-36 137
MS.gene68334.t1 AT2G34070 28.045 353 220 12 84 433 62 383 3.47e-36 137
MS.gene68334.t1 AT3G06080 35.366 246 142 7 87 323 103 340 4.99e-35 133
MS.gene68334.t1 AT1G78710 30.423 355 203 13 87 433 38 356 6.20e-35 133
MS.gene68334.t1 AT5G64470 27.926 376 220 15 80 433 48 394 1.78e-34 133
MS.gene68334.t1 AT5G64470 27.749 382 219 16 80 433 48 400 1.27e-33 130
MS.gene68334.t1 AT2G38320 28.438 320 213 9 117 429 7 317 1.31e-33 129
MS.gene68334.t1 AT1G29050 28.736 348 217 13 87 433 61 378 2.11e-33 129
MS.gene68334.t1 AT5G20680 27.151 372 216 14 86 433 117 457 5.68e-33 130
MS.gene68334.t1 AT5G20680 27.224 371 215 14 87 433 194 533 6.93e-33 130
MS.gene68334.t1 AT5G20680 27.224 371 215 14 87 433 212 551 7.38e-33 130
MS.gene68334.t1 AT5G20680 27.224 371 215 14 87 433 212 551 7.38e-33 130
MS.gene68334.t1 AT5G20680 27.224 371 215 14 87 433 212 551 7.38e-33 130
MS.gene68334.t1 AT5G20680 27.224 371 215 14 87 433 212 551 7.38e-33 130
MS.gene68334.t1 AT5G01620 27.078 373 225 14 81 433 104 449 3.47e-32 127
MS.gene68334.t1 AT5G01620 27.078 373 225 14 81 433 104 449 3.47e-32 127
MS.gene68334.t1 AT5G01620 27.078 373 225 14 81 433 112 457 4.12e-32 127
MS.gene68334.t1 AT2G40160 28.664 307 188 10 30 322 22 311 1.16e-31 123
MS.gene68334.t1 AT3G54260 29.213 356 211 14 87 433 51 374 1.33e-31 124
MS.gene68334.t1 AT5G20590 32.669 251 142 9 87 323 141 378 2.90e-31 124
MS.gene68334.t1 AT3G62390 31.148 244 155 6 87 324 138 374 3.40e-30 121
MS.gene68334.t1 AT5G06230 32.031 256 149 9 84 323 59 305 1.70e-28 114
MS.gene68334.t1 AT5G58600 31.535 241 144 8 86 321 65 289 4.31e-27 110
MS.gene68334.t1 AT2G30900 31.053 190 124 3 87 275 43 226 2.37e-25 105
MS.gene68334.t1 AT2G31110 32.759 174 110 3 87 260 45 211 1.12e-24 103
MS.gene68334.t1 AT1G78710 32.948 173 107 4 87 258 31 195 9.77e-21 92.4
MS.gene68334.t1 AT5G64470 26.460 291 180 12 80 351 48 323 5.84e-20 90.9
MS.gene68334.t1 AT5G64470 27.338 278 168 12 79 337 47 309 1.11e-19 89.7
MS.gene68334.t1 AT3G14850 25.442 283 171 10 158 433 2 251 1.67e-14 73.6

Find 109 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCTTAATCACACTGAAAT+TGG 0.125051 5.2:-25585901 MS.gene68334:CDS
TCCTATCAATTATACTATTA+AGG 0.152448 5.2:+25585842 None:intergenic
AACAAAGATGGTCATGAAAA+TGG 0.212233 5.2:-25586864 None:intergenic
GGCACTTGATGTAACTAAAT+TGG 0.237596 5.2:-25585599 MS.gene68334:CDS
ATGCAAGTGTGTCAGTTTAT+TGG 0.262767 5.2:-25586122 MS.gene68334:CDS
GTGACTACTTCAATGGAAAA+TGG 0.280998 5.2:-25586467 MS.gene68334:CDS
TCCTTCTCCCTACTTCTATA+AGG 0.286857 5.2:+25585718 None:intergenic
AAATACAACTTATTATGATT+TGG 0.295848 5.2:+25586057 None:intergenic
CTGATGCTGATTATCTTTAC+TGG 0.306828 5.2:-25586359 MS.gene68334:CDS
TGAAAGATGGGCAAAGGATA+TGG 0.342119 5.2:-25586022 MS.gene68334:CDS
TTCTTGAGATCATGAAGAAA+TGG 0.362655 5.2:-25585441 MS.gene68334:CDS
AATGGAAAAGGGTACATATA+AGG 0.371725 5.2:+25585547 None:intergenic
TGTGTTCATTGGTGTTTGCC+TGG 0.372187 5.2:-25585490 MS.gene68334:CDS
CCTATCAATTATACTATTAA+GGG 0.374427 5.2:+25585843 None:intergenic
TTGTGCTTTCAATTGGTCAT+TGG 0.379558 5.2:-25585984 MS.gene68334:CDS
ATGAGTTGAAGAAAAGTGTT+AGG 0.387924 5.2:+25586297 None:intergenic
GGAAGCTCAGATTCAGTAGA+TGG 0.390311 5.2:+25586719 None:intergenic
CCACTTGTGTAAACAAGTTC+AGG 0.392892 5.2:+25586186 None:intergenic
CTGATTATCTTTACTGGAGA+TGG 0.393599 5.2:-25586353 MS.gene68334:CDS
TAGATCTTGTTGATGAAAGA+TGG 0.395598 5.2:-25586035 MS.gene68334:CDS
ATTGATAGGAGAGGAAATAA+AGG 0.397784 5.2:-25585829 MS.gene68334:CDS
AAGGTGAAAGGAAGTGAATT+TGG 0.402831 5.2:-25585637 MS.gene68334:CDS
TCAACACCTTGAACAAGAAA+AGG 0.403244 5.2:+25586096 None:intergenic
AAACTAAGCCTTATAGAAGT+AGG 0.405114 5.2:-25585726 MS.gene68334:CDS
AGGTGTAGAGTGGGAGATGA+TGG 0.405251 5.2:+25586682 None:intergenic
ACTCATTGAAAACAAACATA+TGG 0.411083 5.2:-25586280 MS.gene68334:CDS
GTCTTAATCACACTGAAATT+GGG 0.413858 5.2:-25585900 MS.gene68334:CDS
TAAAAGAAGGAGGTGTAGAG+TGG 0.427411 5.2:+25586672 None:intergenic
TGCAAAATGATTGTGTTCAT+TGG 0.427697 5.2:-25585501 MS.gene68334:CDS
ATTTGGAGGGGTTAGGTTTG+AGG 0.439421 5.2:-25585620 MS.gene68334:CDS
ACCCCATTAGCAAATGGAAA+AGG 0.442392 5.2:+25585535 None:intergenic
TCTTGAGATCATGAAGAAAT+GGG 0.442784 5.2:-25585440 MS.gene68334:CDS
CCCACATGTGACTACTTCAA+TGG 0.443622 5.2:-25586474 MS.gene68334:CDS
TTAGGTTGTCATTATTGTGT+TGG 0.447247 5.2:-25585922 MS.gene68334:CDS
GAGTGGAATTGATCTTTGAA+TGG 0.450724 5.2:+25586830 None:intergenic
CATGTTGTTCCATTGTATAA+AGG 0.454390 5.2:+25586426 None:intergenic
CAACACCTTGAACAAGAAAA+GGG 0.457580 5.2:+25586097 None:intergenic
GGCAAAGGATATGGAAAGGA+TGG 0.461465 5.2:-25586013 MS.gene68334:CDS
TAAGGACCTGGATGACCATC+TGG 0.461971 5.2:+25585565 None:intergenic
GTGAAGATAATAAATTTAGA+AGG 0.462452 5.2:-25586164 MS.gene68334:CDS
TGCCTGGACCTATAGATACT+TGG 0.463354 5.2:-25585474 MS.gene68334:CDS
CTTGGAAGCTTACATTCACT+TGG 0.465242 5.2:+25586327 None:intergenic
GCATCCTGCAGTTTACTATG+AGG 0.470478 5.2:-25585956 MS.gene68334:CDS
ACAAAGATGGTCATGAAAAT+GGG 0.471677 5.2:-25586863 None:intergenic
TTGAGACCAGATGGTCATCC+AGG 0.479603 5.2:-25585571 MS.gene68334:CDS
CAAGTGAATGTAAGCTTCCA+AGG 0.484258 5.2:-25586326 MS.gene68334:CDS
TAGATGGAAGAAAGGGAAGA+GGG 0.486400 5.2:+25586735 None:intergenic
TATAAACTAAGAAGAGCAAT+GGG 0.491035 5.2:+25586764 None:intergenic
GGAGAAGGAACTTGAAGGAA+TGG 0.495842 5.2:-25585704 MS.gene68334:CDS
GAAGTGAATTTGGAGGGGTT+AGG 0.498277 5.2:-25585627 MS.gene68334:CDS
GGTGTTGAGAAATCAAATGA+TGG 0.501614 5.2:-25586081 MS.gene68334:CDS
GATTCAGTAGATGGAAGAAA+GGG 0.502270 5.2:+25586728 None:intergenic
TTGTTGGTGATTCTATGGCT+AGG 0.502299 5.2:-25586254 MS.gene68334:CDS
CAGTAAAGATAATCAGCATC+AGG 0.502532 5.2:+25586360 None:intergenic
AGATCTTGTTGATGAAAGAT+GGG 0.502869 5.2:-25586034 MS.gene68334:CDS
AAAAGAAGGAGGTGTAGAGT+GGG 0.507492 5.2:+25586673 None:intergenic
GTTGAAGATGCTAAGGTGAA+AGG 0.508663 5.2:-25585649 MS.gene68334:CDS
CCCTTAATAGTATAATTGAT+AGG 0.509511 5.2:-25585843 MS.gene68334:CDS
GTAGATGGAAGAAAGGGAAG+AGG 0.509775 5.2:+25586734 None:intergenic
TGAAGAAGTTGAAGATGCTA+AGG 0.511290 5.2:-25585656 MS.gene68334:CDS
AGTAGGGAGAAGGAACTTGA+AGG 0.514231 5.2:-25585709 MS.gene68334:CDS
CCATTGAAGTAGTCACATGT+GGG 0.515482 5.2:+25586474 None:intergenic
AACTAAGCCTTATAGAAGTA+GGG 0.515898 5.2:-25585725 MS.gene68334:CDS
CATAAACGTACCTGAAGGAG+GGG 0.517380 5.2:+25586640 None:intergenic
CATCCTGCAGTTTACTATGA+GGG 0.517488 5.2:-25585955 MS.gene68334:CDS
CCTGAACTTGTTTACACAAG+TGG 0.519266 5.2:-25586186 MS.gene68334:CDS
AGATTCAGTAGATGGAAGAA+AGG 0.521045 5.2:+25586727 None:intergenic
AGAAGAGCATAAAGAGTCCA+AGG 0.521608 5.2:+25586788 None:intergenic
CCCCATTAGCAAATGGAAAA+GGG 0.522058 5.2:+25585536 None:intergenic
ATCTCATTCCAAGTATCTAT+AGG 0.524335 5.2:+25585466 None:intergenic
AAAGTGTTAGGCTCAAACCT+TGG 0.534233 5.2:+25586309 None:intergenic
TACCTCACTCTTCACTCTAG+TGG 0.537525 5.2:+25585409 None:intergenic
AGTGGAATTGATCTTTGAAT+GGG 0.540788 5.2:+25586831 None:intergenic
CATTCTCCTTCAAAATGAGC+TGG 0.542110 5.2:+25585772 None:intergenic
TTCCAAGTATCTATAGGTCC+AGG 0.542291 5.2:+25585472 None:intergenic
TCCATTGAAGTAGTCACATG+TGG 0.548479 5.2:+25586473 None:intergenic
GAAGGAGAATGGGATAAAGC+TGG 0.550749 5.2:-25585760 MS.gene68334:CDS
ATGATGGTAGTAGTTGAATG+AGG 0.552151 5.2:+25586698 None:intergenic
TCACCCTCATAGTAAACTGC+AGG 0.552279 5.2:+25585952 None:intergenic
TCTGGAACCCCATTAGCAAA+TGG 0.557935 5.2:+25585529 None:intergenic
TGAAAGGAAGTGAATTTGGA+GGG 0.562220 5.2:-25585633 MS.gene68334:CDS
ACATGTGGAACAATCAAAGA+AGG 0.566099 5.2:-25586408 MS.gene68334:CDS
AAGATAATAAATTTAGAAGG+TGG 0.567467 5.2:-25586161 MS.gene68334:CDS
TTATACAATGGAACAACATG+TGG 0.569031 5.2:-25586423 MS.gene68334:CDS
ATATAAACTAAGAAGAGCAA+TGG 0.579490 5.2:+25586763 None:intergenic
GCCTTATAGAAGTAGGGAGA+AGG 0.580965 5.2:-25585719 MS.gene68334:CDS
TTGGCATTGTTGAGACCAGA+TGG 0.580975 5.2:-25585580 MS.gene68334:CDS
TGCATAAACGTACCTGAAGG+AGG 0.585332 5.2:+25586638 None:intergenic
TGTTGATGAAAGATGGGCAA+AGG 0.591875 5.2:-25586028 MS.gene68334:CDS
ATAAAGGTCCTCTTCTATCA+CGG 0.598847 5.2:+25586442 None:intergenic
GTGAAAGGAAGTGAATTTGG+AGG 0.600937 5.2:-25585634 MS.gene68334:CDS
GTGTTGAGAAATCAAATGAT+GGG 0.601066 5.2:-25586080 MS.gene68334:CDS
GAAAGGAAGTGAATTTGGAG+GGG 0.610769 5.2:-25585632 MS.gene68334:CDS
GAGATCATGAAGAAATGGGA+GGG 0.612763 5.2:-25585436 MS.gene68334:CDS
TAGTGAGATGAGGAAAATTG+AGG 0.613129 5.2:-25585680 MS.gene68334:CDS
AAATGGATCCGTGATAGAAG+AGG 0.613784 5.2:-25586450 MS.gene68334:CDS
GGTGTAGAAGTAAAAGAAGG+AGG 0.614256 5.2:+25586662 None:intergenic
GATGGGCAAAGGATATGGAA+AGG 0.617381 5.2:-25586017 MS.gene68334:CDS
AGAAGAGGACCTTTATACAA+TGG 0.622543 5.2:-25586435 MS.gene68334:CDS
GCATAAACGTACCTGAAGGA+GGG 0.633056 5.2:+25586639 None:intergenic
AAAGGGTACATATAAGGACC+TGG 0.633154 5.2:+25585553 None:intergenic
TGAGATCATGAAGAAATGGG+AGG 0.635992 5.2:-25585437 MS.gene68334:CDS
CATTGAAGTAGTCACATGTG+GGG 0.636248 5.2:+25586475 None:intergenic
GGACAAAATTGTATCAAACA+TGG 0.637951 5.2:-25586387 MS.gene68334:CDS
AATAGTATAATTGATAGGAG+AGG 0.639029 5.2:-25585838 MS.gene68334:CDS
AAGGAATGGATAGTGAGATG+AGG 0.645343 5.2:-25585690 MS.gene68334:CDS
AACACCTTGAACAAGAAAAG+GGG 0.652940 5.2:+25586098 None:intergenic
ATTGAAGTAGTCACATGTGG+GGG 0.680101 5.2:+25586476 None:intergenic
ATAAACGTACCTGAAGGAGG+GGG 0.706780 5.2:+25586641 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATACAACTTATTATGATT+TGG + chr5.2:25586223-25586242 None:intergenic 15.0%
!!! AAAAATGATGTTTTTACAAA+TGG - chr5.2:25585677-25585696 MS.gene68334:CDS 15.0%
!!! AAAATGATGTTTTTACAAAT+GGG - chr5.2:25585678-25585697 MS.gene68334:CDS 15.0%
!! AAGATAATAAATTTAGAAGG+TGG - chr5.2:25586116-25586135 MS.gene68334:CDS 20.0%
!! CCTATCAATTATACTATTAA+GGG + chr5.2:25586437-25586456 None:intergenic 20.0%
!! GTGAAGATAATAAATTTAGA+AGG - chr5.2:25586113-25586132 MS.gene68334:CDS 20.0%
!! TCCTATCAATTATACTATTA+AGG + chr5.2:25586438-25586457 None:intergenic 20.0%
! AATAGTATAATTGATAGGAG+AGG - chr5.2:25586439-25586458 MS.gene68334:CDS 25.0%
! AATGTTCTTTATTGCAGAAA+AGG - chr5.2:25585763-25585782 MS.gene68334:CDS 25.0%
! ACTCATTGAAAACAAACATA+TGG - chr5.2:25585997-25586016 MS.gene68334:CDS 25.0%
! ATATAAACTAAGAAGAGCAA+TGG + chr5.2:25585517-25585536 None:intergenic 25.0%
! CCCTTAATAGTATAATTGAT+AGG - chr5.2:25586434-25586453 MS.gene68334:CDS 25.0%
! TATAAACTAAGAAGAGCAAT+GGG + chr5.2:25585516-25585535 None:intergenic 25.0%
!! GATTTTATTGTGCTTTCAAT+TGG - chr5.2:25586286-25586305 MS.gene68334:CDS 25.0%
!! GTTTTATGATGTATTGAGAA+AGG - chr5.2:25586399-25586418 MS.gene68334:CDS 25.0%
AGTGGAATTGATCTTTGAAT+GGG + chr5.2:25585449-25585468 None:intergenic 30.0%
ATCTCATTCCAAGTATCTAT+AGG + chr5.2:25586814-25586833 None:intergenic 30.0%
ATTGATAGGAGAGGAAATAA+AGG - chr5.2:25586448-25586467 MS.gene68334:CDS 30.0%
CATGTTGTTCCATTGTATAA+AGG + chr5.2:25585854-25585873 None:intergenic 30.0%
GGACAAAATTGTATCAAACA+TGG - chr5.2:25585890-25585909 MS.gene68334:CDS 30.0%
GTCTTAATCACACTGAAATT+GGG - chr5.2:25586377-25586396 MS.gene68334:CDS 30.0%
GTGTTGAGAAATCAAATGAT+GGG - chr5.2:25586197-25586216 MS.gene68334:CDS 30.0%
TCTTGAGATCATGAAGAAAT+GGG - chr5.2:25586837-25586856 MS.gene68334:CDS 30.0%
TGCAAAATGATTGTGTTCAT+TGG - chr5.2:25586776-25586795 MS.gene68334:CDS 30.0%
TTATACAATGGAACAACATG+TGG - chr5.2:25585854-25585873 MS.gene68334:CDS 30.0%
TTCTTGAGATCATGAAGAAA+TGG - chr5.2:25586836-25586855 MS.gene68334:CDS 30.0%
! AAACTAAGCCTTATAGAAGT+AGG - chr5.2:25586551-25586570 MS.gene68334:intron 30.0%
! AACAAACATATGGCTTTTGT+TGG - chr5.2:25586007-25586026 MS.gene68334:CDS 30.0%
! AACTAAGCCTTATAGAAGTA+GGG - chr5.2:25586552-25586571 MS.gene68334:intron 30.0%
! AATGGAAAAGGGTACATATA+AGG + chr5.2:25586733-25586752 None:intergenic 30.0%
! AGATCTTGTTGATGAAAGAT+GGG - chr5.2:25586243-25586262 MS.gene68334:CDS 30.0%
! TAGATCTTGTTGATGAAAGA+TGG - chr5.2:25586242-25586261 MS.gene68334:CDS 30.0%
! TTAGGTTGTCATTATTGTGT+TGG - chr5.2:25586355-25586374 MS.gene68334:CDS 30.0%
!! ATGAGTTGAAGAAAAGTGTT+AGG + chr5.2:25585983-25586002 None:intergenic 30.0%
!! CTTCTATAAGGCTTAGTTTT+TGG + chr5.2:25586550-25586569 None:intergenic 30.0%
!! TCACCAAAAAACTTTTTCCT+TGG - chr5.2:25585472-25585491 MS.gene68334:CDS 30.0%
!!! AGGAAAAAGTTTTTTGGTGA+TGG + chr5.2:25585472-25585491 None:intergenic 30.0%
!!! AGTCCAAGGAAAAAGTTTTT+TGG + chr5.2:25585478-25585497 None:intergenic 30.0%
AACACCTTGAACAAGAAAAG+GGG + chr5.2:25586182-25586201 None:intergenic 35.0%
AAGGTGAAAGGAAGTGAATT+TGG - chr5.2:25586640-25586659 MS.gene68334:intron 35.0%
ACATGTGGAACAATCAAAGA+AGG - chr5.2:25585869-25585888 MS.gene68334:CDS 35.0%
AGAAGAGGACCTTTATACAA+TGG - chr5.2:25585842-25585861 MS.gene68334:CDS 35.0%
AGATTCAGTAGATGGAAGAA+AGG + chr5.2:25585553-25585572 None:intergenic 35.0%
ATAAAGGTCCTCTTCTATCA+CGG + chr5.2:25585838-25585857 None:intergenic 35.0%
ATGATGGTAGTAGTTGAATG+AGG + chr5.2:25585582-25585601 None:intergenic 35.0%
ATGCAAGTGTGTCAGTTTAT+TGG - chr5.2:25586155-25586174 MS.gene68334:CDS 35.0%
CAACACCTTGAACAAGAAAA+GGG + chr5.2:25586183-25586202 None:intergenic 35.0%
CAGTAAAGATAATCAGCATC+AGG + chr5.2:25585920-25585939 None:intergenic 35.0%
CTGATTATCTTTACTGGAGA+TGG - chr5.2:25585924-25585943 MS.gene68334:CDS 35.0%
GAGTGGAATTGATCTTTGAA+TGG + chr5.2:25585450-25585469 None:intergenic 35.0%
GATTCAGTAGATGGAAGAAA+GGG + chr5.2:25585552-25585571 None:intergenic 35.0%
GGCACTTGATGTAACTAAAT+TGG - chr5.2:25586678-25586697 MS.gene68334:CDS 35.0%
GGTCTTAATCACACTGAAAT+TGG - chr5.2:25586376-25586395 MS.gene68334:CDS 35.0%
GTGACTACTTCAATGGAAAA+TGG - chr5.2:25585810-25585829 MS.gene68334:CDS 35.0%
TAGTGAGATGAGGAAAATTG+AGG - chr5.2:25586597-25586616 MS.gene68334:intron 35.0%
TCAACACCTTGAACAAGAAA+AGG + chr5.2:25586184-25586203 None:intergenic 35.0%
TGAAAGGAAGTGAATTTGGA+GGG - chr5.2:25586644-25586663 MS.gene68334:intron 35.0%
TGAAGAAGTTGAAGATGCTA+AGG - chr5.2:25586621-25586640 MS.gene68334:intron 35.0%
TTGTGCTTTCAATTGGTCAT+TGG - chr5.2:25586293-25586312 MS.gene68334:CDS 35.0%
! CTGATGCTGATTATCTTTAC+TGG - chr5.2:25585918-25585937 MS.gene68334:CDS 35.0%
! GGTGTTGAGAAATCAAATGA+TGG - chr5.2:25586196-25586215 MS.gene68334:CDS 35.0%
! TATGAGGGTGATTCAGTTTT+AGG - chr5.2:25586337-25586356 MS.gene68334:CDS 35.0%
! TTTTCACCAGCTCATTTTGA+AGG - chr5.2:25586499-25586518 MS.gene68334:CDS 35.0%
!! ACCCTTTTCCATTTGCTAAT+GGG - chr5.2:25586740-25586759 MS.gene68334:CDS 35.0%
!! AGCTCATTTTGAAGGAGAAT+GGG - chr5.2:25586507-25586526 MS.gene68334:intron 35.0%
!! TACCCTTTTCCATTTGCTAA+TGG - chr5.2:25586739-25586758 MS.gene68334:CDS 35.0%
!!! AAAGTTTTTTGGTGATGGAG+TGG + chr5.2:25585467-25585486 None:intergenic 35.0%
AAATGGATCCGTGATAGAAG+AGG - chr5.2:25585827-25585846 MS.gene68334:CDS 40.0%
AAGGAATGGATAGTGAGATG+AGG - chr5.2:25586587-25586606 MS.gene68334:intron 40.0%
ACCCCATTAGCAAATGGAAA+AGG + chr5.2:25586745-25586764 None:intergenic 40.0%
ATTGAAGTAGTCACATGTGG+GGG + chr5.2:25585804-25585823 None:intergenic 40.0%
CAAGTGAATGTAAGCTTCCA+AGG - chr5.2:25585951-25585970 MS.gene68334:CDS 40.0%
CATCCTGCAGTTTACTATGA+GGG - chr5.2:25586322-25586341 MS.gene68334:CDS 40.0%
CATTCTCCTTCAAAATGAGC+TGG + chr5.2:25586508-25586527 None:intergenic 40.0%
CATTGAAGTAGTCACATGTG+GGG + chr5.2:25585805-25585824 None:intergenic 40.0%
CCACTTGTGTAAACAAGTTC+AGG + chr5.2:25586094-25586113 None:intergenic 40.0%
CCATTGAAGTAGTCACATGT+GGG + chr5.2:25585806-25585825 None:intergenic 40.0%
CCCCATTAGCAAATGGAAAA+GGG + chr5.2:25586744-25586763 None:intergenic 40.0%
CTTGGAAGCTTACATTCACT+TGG + chr5.2:25585953-25585972 None:intergenic 40.0%
GAAAGGAAGTGAATTTGGAG+GGG - chr5.2:25586645-25586664 MS.gene68334:intron 40.0%
GAGATCATGAAGAAATGGGA+GGG - chr5.2:25586841-25586860 MS.gene68334:CDS 40.0%
GGTGTAGAAGTAAAAGAAGG+AGG + chr5.2:25585618-25585637 None:intergenic 40.0%
GTATGCATAAACGTACCTGA+AGG + chr5.2:25585645-25585664 None:intergenic 40.0%
GTGAAAGGAAGTGAATTTGG+AGG - chr5.2:25586643-25586662 MS.gene68334:intron 40.0%
GTTGAAGATGCTAAGGTGAA+AGG - chr5.2:25586628-25586647 MS.gene68334:intron 40.0%
TAGATGGAAGAAAGGGAAGA+GGG + chr5.2:25585545-25585564 None:intergenic 40.0%
TCCATTGAAGTAGTCACATG+TGG + chr5.2:25585807-25585826 None:intergenic 40.0%
TCCTTCTCCCTACTTCTATA+AGG + chr5.2:25586562-25586581 None:intergenic 40.0%
TGAAAGATGGGCAAAGGATA+TGG - chr5.2:25586255-25586274 MS.gene68334:CDS 40.0%
TGAGATCATGAAGAAATGGG+AGG - chr5.2:25586840-25586859 MS.gene68334:CDS 40.0%
TTCCAAGTATCTATAGGTCC+AGG + chr5.2:25586808-25586827 None:intergenic 40.0%
! AAAAGAAGGAGGTGTAGAGT+GGG + chr5.2:25585607-25585626 None:intergenic 40.0%
! AAAGGGTACATATAAGGACC+TGG + chr5.2:25586727-25586746 None:intergenic 40.0%
! AAAGTGTTAGGCTCAAACCT+TGG + chr5.2:25585971-25585990 None:intergenic 40.0%
! AGAAGAGCATAAAGAGTCCA+AGG + chr5.2:25585492-25585511 None:intergenic 40.0%
! CCTGAACTTGTTTACACAAG+TGG - chr5.2:25586091-25586110 MS.gene68334:CDS 40.0%
! TAAAAGAAGGAGGTGTAGAG+TGG + chr5.2:25585608-25585627 None:intergenic 40.0%
! TGTTGATGAAAGATGGGCAA+AGG - chr5.2:25586249-25586268 MS.gene68334:CDS 40.0%
!! CAGCTCATTTTGAAGGAGAA+TGG - chr5.2:25586506-25586525 MS.gene68334:intron 40.0%
!! CCCTTTTCCATTTGCTAATG+GGG - chr5.2:25586741-25586760 MS.gene68334:CDS 40.0%
!! TTGTTGGTGATTCTATGGCT+AGG - chr5.2:25586023-25586042 MS.gene68334:CDS 40.0%
!!! GGCTTTTGTTGGTGATTCTA+TGG - chr5.2:25586018-25586037 MS.gene68334:CDS 40.0%
AGTAGGGAGAAGGAACTTGA+AGG - chr5.2:25586568-25586587 MS.gene68334:intron 45.0%
ATAAACGTACCTGAAGGAGG+GGG + chr5.2:25585639-25585658 None:intergenic 45.0%
ATTTGGAGGGGTTAGGTTTG+AGG - chr5.2:25586657-25586676 MS.gene68334:CDS 45.0%
CATAAACGTACCTGAAGGAG+GGG + chr5.2:25585640-25585659 None:intergenic 45.0%
CCCACATGTGACTACTTCAA+TGG - chr5.2:25585803-25585822 MS.gene68334:CDS 45.0%
GAAGGAGAATGGGATAAAGC+TGG - chr5.2:25586517-25586536 MS.gene68334:intron 45.0%
GAAGTGAATTTGGAGGGGTT+AGG - chr5.2:25586650-25586669 MS.gene68334:intron 45.0%
GATGGGCAAAGGATATGGAA+AGG - chr5.2:25586260-25586279 MS.gene68334:CDS 45.0%
GCATAAACGTACCTGAAGGA+GGG + chr5.2:25585641-25585660 None:intergenic 45.0%
GCATCCTGCAGTTTACTATG+AGG - chr5.2:25586321-25586340 MS.gene68334:CDS 45.0%
GCCTTATAGAAGTAGGGAGA+AGG - chr5.2:25586558-25586577 MS.gene68334:intron 45.0%
GGAAGCTCAGATTCAGTAGA+TGG + chr5.2:25585561-25585580 None:intergenic 45.0%
GGAGAAGGAACTTGAAGGAA+TGG - chr5.2:25586573-25586592 MS.gene68334:intron 45.0%
GGCAAAGGATATGGAAAGGA+TGG - chr5.2:25586264-25586283 MS.gene68334:CDS 45.0%
GTAGATGGAAGAAAGGGAAG+AGG + chr5.2:25585546-25585565 None:intergenic 45.0%
TCACCCTCATAGTAAACTGC+AGG + chr5.2:25586328-25586347 None:intergenic 45.0%
TGCATAAACGTACCTGAAGG+AGG + chr5.2:25585642-25585661 None:intergenic 45.0%
TGCCTGGACCTATAGATACT+TGG - chr5.2:25586803-25586822 MS.gene68334:CDS 45.0%
TTGGCATTGTTGAGACCAGA+TGG - chr5.2:25586697-25586716 MS.gene68334:CDS 45.0%
! CAATCATTTTGCACACGCTC+TGG + chr5.2:25586769-25586788 None:intergenic 45.0%
! GGGGGTGTAGAAGTAAAAGA+AGG + chr5.2:25585621-25585640 None:intergenic 45.0%
! TCTGGAACCCCATTAGCAAA+TGG + chr5.2:25586751-25586770 None:intergenic 45.0%
! TGGTCCCCTTTTCTTGTTCA+AGG - chr5.2:25586175-25586194 MS.gene68334:CDS 45.0%
! TGTGTTCATTGGTGTTTGCC+TGG - chr5.2:25586787-25586806 MS.gene68334:CDS 45.0%
AGGTGTAGAGTGGGAGATGA+TGG + chr5.2:25585598-25585617 None:intergenic 50.0%
TAAGGACCTGGATGACCATC+TGG + chr5.2:25586715-25586734 None:intergenic 50.0%
TTGAGACCAGATGGTCATCC+AGG - chr5.2:25586706-25586725 MS.gene68334:CDS 50.0%
ACTTCTACACCCCCTCCTTC+AGG - chr5.2:25585627-25585646 MS.gene68334:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 25585420 25586879 25585420 ID=MS.gene68334
chr5.2 mRNA 25585420 25586879 25585420 ID=MS.gene68334.t1;Parent=MS.gene68334
chr5.2 exon 25586651 25586879 25586651 ID=MS.gene68334.t1.exon1;Parent=MS.gene68334.t1
chr5.2 CDS 25586651 25586879 25586651 ID=cds.MS.gene68334.t1;Parent=MS.gene68334.t1
chr5.2 exon 25585420 25586519 25585420 ID=MS.gene68334.t1.exon2;Parent=MS.gene68334.t1
chr5.2 CDS 25585420 25586519 25585420 ID=cds.MS.gene68334.t1;Parent=MS.gene68334.t1
Gene Sequence

>MS.gene68334

ATGGTCATGAAAATGGGTATTTCAAACCCATTCAAAGATCAATTCCACTCCATCACCAAAAAACTTTTTCCTTGGACTCTTTATGCTCTTCTTCCCATTGCTCTTCTTAGTTTATATCTCTACCCTCTTCCCTTTCTTCCATCTACTGAATCTGAGCTTCCTCATTCAACTACTACCATCATCTCCCACTCTACACCTCCTTCTTTTACTTCTACACCCCCTCCTTCAGGTACGTTTATGCATACTCTTATCTAGTTAAAAATGATGTTTTTACAAATGGGTTTTGTCTTAAATCTAGATTAGTAACTTCAGATGTAAGATTTGTTAGTTTTTTTAACATTTAAATGTTCTTTATTGCAGAAAAGGAAAATACTTTTGACACCCCCACATGTGACTACTTCAATGGAAAATGGATCCGTGATAGAAGAGGACCTTTATACAATGGAACAACATGTGGAACAATCAAAGAAGGACAAAATTGTATCAAACATGGACGACCTGATGCTGATTATCTTTACTGGAGATGGAAACCAAGTGAATGTAAGCTTCCAAGGTTTGAGCCTAACACTTTTCTTCAACTCATTGAAAACAAACATATGGCTTTTGTTGGTGATTCTATGGCTAGGAATCAGTTAGAGTCACTTCTTTGTTTGTTATCTAGTGTTTCAACACCTGAACTTGTTTACACAAGTGGTGAAGATAATAAATTTAGAAGGTGGCATTTTGATTCTCATAATGCAAGTGTGTCAGTTTATTGGTCCCCTTTTCTTGTTCAAGGTGTTGAGAAATCAAATGATGGGCCAAATCATAATAAGTTGTATTTAGATCTTGTTGATGAAAGATGGGCAAAGGATATGGAAAGGATGGATTTTATTGTGCTTTCAATTGGTCATTGGTTTTTGCATCCTGCAGTTTACTATGAGGGTGATTCAGTTTTAGGTTGTCATTATTGTGTTGGTCTTAATCACACTGAAATTGGGTTTTATGATGTATTGAGAAAGGCTTTAAGAACTACCCTTAATAGTATAATTGATAGGAGAGGAAATAAAGGTAGTAAAATTGATGTACTTGTTACAACATTTTCACCAGCTCATTTTGAAGGAGAATGGGATAAAGCTGGTGCTTGTCCAAAAACTAAGCCTTATAGAAGTAGGGAGAAGGAACTTGAAGGAATGGATAGTGAGATGAGGAAAATTGAGGTTGAAGAAGTTGAAGATGCTAAGGTGAAAGGAAGTGAATTTGGAGGGGTTAGGTTTGAGGCACTTGATGTAACTAAATTGGCATTGTTGAGACCAGATGGTCATCCAGGTCCTTATATGTACCCTTTTCCATTTGCTAATGGGGTTCCAGAGCGTGTGCAAAATGATTGTGTTCATTGGTGTTTGCCTGGACCTATAGATACTTGGAATGAGATTTTTCTTGAGATCATGAAGAAATGGGAGGGACATCCACTAGAGTGA
Protein sequence

>MS.gene68334.t1

MVMKMGISNPFKDQFHSITKKLFPWTLYALLPIALLSLYLYPLPFLPSTESELPHSTTTIISHSTPPSFTSTPPPSEKENTFDTPTCDYFNGKWIRDRRGPLYNGTTCGTIKEGQNCIKHGRPDADYLYWRWKPSECKLPRFEPNTFLQLIENKHMAFVGDSMARNQLESLLCLLSSVSTPELVYTSGEDNKFRRWHFDSHNASVSVYWSPFLVQGVEKSNDGPNHNKLYLDLVDERWAKDMERMDFIVLSIGHWFLHPAVYYEGDSVLGCHYCVGLNHTEIGFYDVLRKALRTTLNSIIDRRGNKGSKIDVLVTTFSPAHFEGEWDKAGACPKTKPYRSREKELEGMDSEMRKIEVEEVEDAKVKGSEFGGVRFEALDVTKLALLRPDGHPGPYMYPFPFANGVPERVQNDCVHWCLPGPIDTWNEIFLEIMKKWEGHPLE