Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68399.t1 | XP_003621884.1 | 92.5 | 80 | 6 | 0 | 3 | 82 | 96 | 175 | 9.60E-30 | 139 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68399.t1 | G7KXT7 | 92.5 | 80 | 6 | 0 | 3 | 82 | 96 | 175 | 6.9e-30 | 139.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049873 | MS.gene68399 | 0.813404 | 2.60E-51 | -1.69E-46 |
| MS.gene052519 | MS.gene68399 | 0.808402 | 3.15E-50 | -1.69E-46 |
| MS.gene052582 | MS.gene68399 | 0.807785 | 4.27E-50 | -1.69E-46 |
| MS.gene052584 | MS.gene68399 | 0.808428 | 3.11E-50 | -1.69E-46 |
| MS.gene053226 | MS.gene68399 | 0.808067 | 3.72E-50 | -1.69E-46 |
| MS.gene05954 | MS.gene68399 | 0.833034 | 6.72E-56 | -1.69E-46 |
| MS.gene060429 | MS.gene68399 | 0.806708 | 7.21E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68399.t1 | MTR_7g024560 | 92.500 | 80 | 6 | 0 | 3 | 82 | 96 | 175 | 3.02e-35 | 121 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68399.t1 | AT3G04020 | 40.244 | 82 | 47 | 2 | 3 | 82 | 95 | 176 | 3.63e-12 | 58.9 |
| MS.gene68399.t1 | AT3G04020 | 40.506 | 79 | 45 | 2 | 3 | 79 | 30 | 108 | 2.07e-11 | 57.8 |
| MS.gene68399.t1 | AT3G04020 | 40.506 | 79 | 45 | 2 | 3 | 79 | 27 | 105 | 2.08e-11 | 57.4 |
| MS.gene68399.t1 | AT3G04020 | 40.506 | 79 | 45 | 2 | 3 | 79 | 95 | 173 | 2.37e-11 | 57.8 |
Find 18 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAGCTCGGGTTGCTTTATC+TGG | 0.129082 | 1.3:-27066608 | MS.gene68399:CDS |
| TGATAATCTTCAGGAACTAT+TGG | 0.336357 | 1.3:-27065630 | MS.gene68399:CDS |
| AAGTTTAGTTACACTGTGTC+TGG | 0.387940 | 1.3:-27065689 | MS.gene68399:CDS |
| CAGCTCGGGTTGCTTTATCT+GGG | 0.418686 | 1.3:-27066607 | MS.gene68399:CDS |
| GATAATCTTCAGGAACTATT+GGG | 0.438617 | 1.3:-27065629 | MS.gene68399:CDS |
| AACTTTCTCTGTGAGCGCCT+TGG | 0.466325 | 1.3:+27065748 | None:intergenic |
| ATCGCGAAGTGATAATCTTC+AGG | 0.479237 | 1.3:-27065639 | MS.gene68399:CDS |
| CATGCTGCAGTTACCAGCTC+GGG | 0.495177 | 1.3:-27066621 | MS.gene68399:CDS |
| TCTTCCTCTCAATGAAGATA+AGG | 0.528212 | 1.3:-27066579 | MS.gene68399:intron |
| CCAGATAAAGCAACCCGAGC+TGG | 0.528822 | 1.3:+27066608 | None:intergenic |
| ACATGCTGCAGTTACCAGCT+CGG | 0.544322 | 1.3:-27066622 | MS.gene68399:CDS |
| AATACCTTATCTTCATTGAG+AGG | 0.547218 | 1.3:+27066575 | None:intergenic |
| TCTGGAGTACTAAGTTCTAG+TGG | 0.548859 | 1.3:-27065671 | MS.gene68399:CDS |
| CTGGAGTACTAAGTTCTAGT+GGG | 0.585327 | 1.3:-27065670 | MS.gene68399:CDS |
| TCATGAAGTCATACGATCTG+AGG | 0.586427 | 1.3:-27065726 | MS.gene68399:CDS |
| AGCTCGGGTTGCTTTATCTG+GGG | 0.593276 | 1.3:-27066606 | MS.gene68399:CDS |
| ATATAGATTTAAAACGAGGT+AGG | 0.612747 | 1.3:-27065585 | None:intergenic |
| CAGTATATAGATTTAAAACG+AGG | 0.627862 | 1.3:-27065589 | MS.gene68399:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CAAGTTTAATTTAATATTAA+GGG | - | chr1.3:27066345-27066364 | MS.gene68399:intron | 10.0% |
| !! | ATTAAATATCTTTATGTGTA+TGG | - | chr1.3:27066156-27066175 | MS.gene68399:intron | 15.0% |
| !! | CCTTAATATTAAATTAAACT+TGG | + | chr1.3:27066347-27066366 | None:intergenic | 15.0% |
| !!! | CCAAGTTTAATTTAATATTA+AGG | - | chr1.3:27066344-27066363 | MS.gene68399:intron | 15.0% |
| !! | AAGCTATATAAATAAGTAGA+AGG | + | chr1.3:27065852-27065871 | None:intergenic | 20.0% |
| !! | AATATTAAATTAAACTTGGC+TGG | + | chr1.3:27066343-27066362 | None:intergenic | 20.0% |
| !!! | ATAATATACACTCTATTTTC+TGG | + | chr1.3:27066188-27066207 | None:intergenic | 20.0% |
| !!! | ATATACTGAATATTTTTCAG+TGG | + | chr1.3:27066605-27066624 | None:intergenic | 20.0% |
| ! | AAATAAGTAGAAGGTTTGAT+GGG | + | chr1.3:27065843-27065862 | None:intergenic | 25.0% |
| ! | AAATATCTTTATGTGTATGG+AGG | - | chr1.3:27066159-27066178 | MS.gene68399:intron | 25.0% |
| ! | AGGAGAGTAATTAATAGTTA+GGG | + | chr1.3:27066064-27066083 | None:intergenic | 25.0% |
| ! | ATATTGTAAAGCGACATAAA+TGG | + | chr1.3:27066007-27066026 | None:intergenic | 25.0% |
| ! | ATGTTAACTTCCTGAATATT+CGG | - | chr1.3:27065763-27065782 | MS.gene68399:intron | 25.0% |
| ! | TAAATAAGTAGAAGGTTTGA+TGG | + | chr1.3:27065844-27065863 | None:intergenic | 25.0% |
| ! | TATTGTAAAGCGACATAAAT+GGG | + | chr1.3:27066006-27066025 | None:intergenic | 25.0% |
| ! | TTCAAATATCGAAATACAGT+TGG | + | chr1.3:27066087-27066106 | None:intergenic | 25.0% |
| !! | CAGTATATAGATTTAAAACG+AGG | - | chr1.3:27066617-27066636 | MS.gene68399:CDS | 25.0% |
| !! | GTTAGTAGATTTTGTTAGTT+AGG | + | chr1.3:27065899-27065918 | None:intergenic | 25.0% |
| !! | TTTTGCATATTCTTTTGCTA+TGG | + | chr1.3:27065739-27065758 | None:intergenic | 25.0% |
| AAATATCGAAATACAGTTGG+AGG | + | chr1.3:27066084-27066103 | None:intergenic | 30.0% | |
| AATACCTTATCTTCATTGAG+AGG | + | chr1.3:27065634-27065653 | None:intergenic | 30.0% | |
| CTCACATTAGAAAAAGAAGA+TGG | + | chr1.3:27066293-27066312 | None:intergenic | 30.0% | |
| GAGGAGAGTAATTAATAGTT+AGG | + | chr1.3:27066065-27066084 | None:intergenic | 30.0% | |
| GATAATCTTCAGGAACTATT+GGG | - | chr1.3:27066577-27066596 | MS.gene68399:intron | 30.0% | |
| TGATAATCTTCAGGAACTAT+TGG | - | chr1.3:27066576-27066595 | MS.gene68399:intron | 30.0% | |
| TGTTAACTTCCTGAATATTC+GGG | - | chr1.3:27065764-27065783 | MS.gene68399:intron | 30.0% | |
| TTGGAATGTGATAAATCTGA+TGG | + | chr1.3:27065921-27065940 | None:intergenic | 30.0% | |
| AAGTTTAGTTACACTGTGTC+TGG | - | chr1.3:27066517-27066536 | MS.gene68399:intron | 35.0% | |
| AATTGATGCTTGGTTGATCT+TGG | - | chr1.3:27066429-27066448 | MS.gene68399:intron | 35.0% | |
| CGCTGTAGATTTGAAAACTA+TGG | - | chr1.3:27066127-27066146 | MS.gene68399:intron | 35.0% | |
| GACTTACATTGTGAGACAAA+GGG | + | chr1.3:27065964-27065983 | None:intergenic | 35.0% | |
| GTAATTGACACTCGTGAAAT+TGG | + | chr1.3:27065940-27065959 | None:intergenic | 35.0% | |
| TCTTCCTCTCAATGAAGATA+AGG | - | chr1.3:27065627-27065646 | MS.gene68399:CDS | 35.0% | |
| TGACTTACATTGTGAGACAA+AGG | + | chr1.3:27065965-27065984 | None:intergenic | 35.0% | |
| TTGTTCTCGAAATTGATGCT+TGG | - | chr1.3:27066419-27066438 | MS.gene68399:intron | 35.0% | |
| ATCGCGAAGTGATAATCTTC+AGG | - | chr1.3:27066567-27066586 | MS.gene68399:intron | 40.0% | |
| GCTGGAGTATAAGTGAAAGT+AGG | + | chr1.3:27066325-27066344 | None:intergenic | 40.0% | |
| TCATGAAGTCATACGATCTG+AGG | - | chr1.3:27066480-27066499 | MS.gene68399:intron | 40.0% | |
| TGATTCAAATCCTCACCGAA+AGG | - | chr1.3:27065810-27065829 | MS.gene68399:intron | 40.0% | |
| TTGATGGGAGTTAGTCCTTT+CGG | + | chr1.3:27065828-27065847 | None:intergenic | 40.0% | |
| TTTCGGTGAGGATTTGAATC+AGG | + | chr1.3:27065811-27065830 | None:intergenic | 40.0% | |
| ! | CTGGAGTACTAAGTTCTAGT+GGG | - | chr1.3:27066536-27066555 | MS.gene68399:intron | 40.0% |
| ! | TCTGGAGTACTAAGTTCTAG+TGG | - | chr1.3:27066535-27066554 | MS.gene68399:intron | 40.0% |
| CTGAGATCTCCCGAATATTC+AGG | + | chr1.3:27065776-27065795 | None:intergenic | 45.0% | |
| ! | TGCTTGGTTGATCTTGGTGT+AGG | - | chr1.3:27066435-27066454 | MS.gene68399:intron | 45.0% |
| AACTTTCTCTGTGAGCGCCT+TGG | + | chr1.3:27066461-27066480 | None:intergenic | 50.0% | |
| TTCGGGAGATCTCAGACTCT+TGG | - | chr1.3:27065781-27065800 | MS.gene68399:intron | 50.0% | |
| ! | AGCTCGGGTTGCTTTATCTG+GGG | - | chr1.3:27065600-27065619 | MS.gene68399:CDS | 50.0% |
| ! | CAGCTCGGGTTGCTTTATCT+GGG | - | chr1.3:27065599-27065618 | MS.gene68399:CDS | 50.0% |
| ! | GTTGATCTTGGTGTAGGCCA+AGG | - | chr1.3:27066441-27066460 | MS.gene68399:intron | 50.0% |
| CCAGATAAAGCAACCCGAGC+TGG | + | chr1.3:27065601-27065620 | None:intergenic | 55.0% | |
| GGGAGTTAGTCCTTTCGGTG+AGG | + | chr1.3:27065823-27065842 | None:intergenic | 55.0% | |
| ! | CCAGCTCGGGTTGCTTTATC+TGG | - | chr1.3:27065598-27065617 | MS.gene68399:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 27065586 | 27066642 | 27065586 | ID=MS.gene68399 |
| chr1.3 | mRNA | 27065586 | 27066642 | 27065586 | ID=MS.gene68399.t1;Parent=MS.gene68399 |
| chr1.3 | exon | 27066580 | 27066642 | 27066580 | ID=MS.gene68399.t1.exon1;Parent=MS.gene68399.t1 |
| chr1.3 | CDS | 27066580 | 27066642 | 27066580 | ID=cds.MS.gene68399.t1;Parent=MS.gene68399.t1 |
| chr1.3 | exon | 27065586 | 27065771 | 27065586 | ID=MS.gene68399.t1.exon2;Parent=MS.gene68399.t1 |
| chr1.3 | CDS | 27065586 | 27065771 | 27065586 | ID=cds.MS.gene68399.t1;Parent=MS.gene68399.t1 |
| Gene Sequence |
| Protein sequence |
