Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68407.t1 | XP_024630846.1 | 99.2 | 479 | 4 | 0 | 1 | 479 | 6 | 484 | 1.20E-273 | 951.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68407.t1 | Q9AV81 | 74.2 | 481 | 119 | 3 | 1 | 479 | 6 | 483 | 5.7e-208 | 724.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68407.t1 | G7I632 | 99.2 | 479 | 4 | 0 | 1 | 479 | 4 | 482 | 8.6e-274 | 951.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049180 | MS.gene68407 | 0.844754 | 6.21E-59 | -1.69E-46 |
| MS.gene053443 | MS.gene68407 | 0.82523 | 5.25E-54 | -1.69E-46 |
| MS.gene056632 | MS.gene68407 | 0.825535 | 4.45E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 99 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATCAATCGAATAGGATTT+TGG | 0.110325 | 1.1:+27756265 | MS.gene68407:intron |
| GACTGCAATGCTGCTCTTTC+CGG | 0.154596 | 1.1:+27759492 | MS.gene68407:CDS |
| GCATTAGCTGTCACAGAATT+TGG | 0.171506 | 1.1:-27759189 | None:intergenic |
| ACAGAATTTGGAGTAGAAAT+AGG | 0.203246 | 1.1:-27759177 | None:intergenic |
| CATCCCGATGGTCTTATTCT+TGG | 0.266936 | 1.1:+27762741 | MS.gene68407:CDS |
| AAAACTCTTCCTGATTTATC+TGG | 0.271266 | 1.1:+27763894 | MS.gene68407:CDS |
| GTGCAGGCAGCAAGCATTCC+TGG | 0.291861 | 1.1:+27756516 | MS.gene68407:CDS |
| TCTTGATATCCTTCATTCTA+AGG | 0.302903 | 1.1:+27759797 | MS.gene68407:CDS |
| TTTGGGAAGTGTCCAGTTAC+AGG | 0.305772 | 1.1:+27756283 | MS.gene68407:CDS |
| CGTGATTGCAAGGCTTAAAA+AGG | 0.320139 | 1.1:+27759107 | MS.gene68407:CDS |
| TTTGACACCACAGGCTCATC+TGG | 0.321677 | 1.1:-27753889 | None:intergenic |
| GGATCCTACCTTGCAGTTGC+AGG | 0.322521 | 1.1:+27763311 | MS.gene68407:CDS |
| ATTTCAGATTCCTGCGACTT+TGG | 0.324712 | 1.1:+27759695 | MS.gene68407:intron |
| CAGATTCTCTTGTTAAGATC+TGG | 0.328133 | 1.1:+27762775 | MS.gene68407:CDS |
| GTTGGAGTTGCTGAGTCATA+TGG | 0.342835 | 1.1:-27763165 | None:intergenic |
| CAGTTGCAGGCTCTGATGTT+AGG | 0.348094 | 1.1:+27763324 | MS.gene68407:CDS |
| GCAAATGTTGCTAAGTTTGA+TGG | 0.357923 | 1.1:+27762907 | MS.gene68407:CDS |
| GGAATGCTTGCTGCCTGCAC+TGG | 0.360377 | 1.1:-27756513 | None:intergenic |
| CTCATGATGGTGTAAAGCTT+TGG | 0.362749 | 1.1:+27763106 | MS.gene68407:CDS |
| TTTGTGACTGCATCACTTGA+TGG | 0.370664 | 1.1:+27762399 | MS.gene68407:CDS |
| TGCAGTCACAAAGTAATTAT+TGG | 0.371362 | 1.1:-27762388 | None:intergenic |
| CTTGCTGCCTGCACTGGTCT+AGG | 0.374275 | 1.1:-27756507 | None:intergenic |
| ACTGCAATGCTGCTCTTTCC+GGG | 0.374696 | 1.1:+27759493 | MS.gene68407:CDS |
| GGATGTAATTTCTTCGCATT+AGG | 0.388848 | 1.1:-27759435 | None:intergenic |
| ACTCAGCGGTCACTCTAAAA+AGG | 0.394859 | 1.1:+27760080 | MS.gene68407:CDS |
| AAGCGTGAAGTTTGTAGGTC+AGG | 0.396085 | 1.1:+27760186 | MS.gene68407:CDS |
| CTTAAACAAAACTAGCAAAC+AGG | 0.402528 | 1.1:+27759764 | MS.gene68407:CDS |
| CTTCCTGATTTATCTGGAAC+AGG | 0.403899 | 1.1:+27763900 | MS.gene68407:CDS |
| CAGGGTGAATCTATCATAAC+TGG | 0.405085 | 1.1:+27760205 | MS.gene68407:CDS |
| GATATTGTGCCGATCAAAAC+AGG | 0.410359 | 1.1:+27756325 | MS.gene68407:CDS |
| GTAAGCGGCTCTCCTGTAAC+TGG | 0.411774 | 1.1:-27756295 | None:intergenic |
| TCATGATGGTGTAAAGCTTT+GGG | 0.421276 | 1.1:+27763107 | MS.gene68407:CDS |
| CTGCTTGCCGCGTGATTGCA+AGG | 0.421314 | 1.1:+27759097 | MS.gene68407:CDS |
| TATTGCAGACAGTTCGTTTG+TGG | 0.428520 | 1.1:+27760652 | MS.gene68407:intron |
| ATTAATCGAGAGGCATATAA+TGG | 0.430404 | 1.1:+27753939 | MS.gene68407:CDS |
| CATAACTGGTTCAGCAGATA+AGG | 0.431703 | 1.1:+27760219 | MS.gene68407:CDS |
| TGTTAGCTCAGAAATTATAC+TGG | 0.433157 | 1.1:-27759469 | None:intergenic |
| TTACAGGACATAATCGCAAC+TGG | 0.446375 | 1.1:+27760000 | MS.gene68407:intron |
| ATTATACTGGAAGATATTCC+AGG | 0.453120 | 1.1:-27759456 | None:intergenic |
| CAGAATTTGGAGTAGAAATA+GGG | 0.462176 | 1.1:-27759176 | None:intergenic |
| CTGCATCACTTGATGGCAGC+TGG | 0.462700 | 1.1:+27762406 | MS.gene68407:CDS |
| TACAGGAGAGCCGCTTACTC+TGG | 0.463440 | 1.1:+27756300 | MS.gene68407:CDS |
| AGATTCTCTTGTTAAGATCT+GGG | 0.470622 | 1.1:+27762776 | MS.gene68407:CDS |
| GAGTCAATACCAGAGTTTGT+TGG | 0.471796 | 1.1:-27763183 | None:intergenic |
| GCAAGCATTCCTGGCATGCT+TGG | 0.474027 | 1.1:+27756525 | MS.gene68407:CDS |
| GTAACAAGCGTGAAGTTTGT+AGG | 0.474487 | 1.1:+27760181 | MS.gene68407:CDS |
| GTAGTATCCATTCTCAGAAA+AGG | 0.476806 | 1.1:-27762956 | None:intergenic |
| ATGCTCCTGTTCATAGCAAT+GGG | 0.482893 | 1.1:+27759208 | MS.gene68407:CDS |
| AGACAGTTCGTTTGTGGCAA+GGG | 0.486431 | 1.1:+27760658 | MS.gene68407:intron |
| AGCTGCAGATGTATAGCCTT+CGG | 0.487652 | 1.1:-27762715 | None:intergenic |
| ATGCTGCTCTTTCCGGGAAG+AGG | 0.487878 | 1.1:+27759499 | MS.gene68407:CDS |
| CTCATTTATAGGAATGGGAT+GGG | 0.490049 | 1.1:+27756770 | MS.gene68407:intron |
| GATGGTCTTATTCTTGGAAC+TGG | 0.491366 | 1.1:+27762747 | MS.gene68407:CDS |
| CAGACAGTTCGTTTGTGGCA+AGG | 0.499376 | 1.1:+27760657 | MS.gene68407:intron |
| AAAGTAATACCTTAGAATGA+AGG | 0.500411 | 1.1:-27759806 | None:intergenic |
| AATGCTCCTGTTCATAGCAA+TGG | 0.505400 | 1.1:+27759207 | MS.gene68407:CDS |
| TGGAACATTCCAAGCATGCC+AGG | 0.505625 | 1.1:-27756534 | None:intergenic |
| AGCTGCTGATGACGAGGAGT+TGG | 0.507322 | 1.1:+27759410 | MS.gene68407:intron |
| CCTCATTTATAGGAATGGGA+TGG | 0.507870 | 1.1:+27756769 | MS.gene68407:intron |
| TTGGAGTTGCTGAGTCATAT+GGG | 0.509156 | 1.1:-27763164 | None:intergenic |
| GTACCTGTTCCAGATAAATC+AGG | 0.512887 | 1.1:-27763903 | None:intergenic |
| CAAAGTAATTATTGGTGGCA+TGG | 0.525985 | 1.1:-27762380 | None:intergenic |
| CTAAGTTTGATGGACATGTC+GGG | 0.530267 | 1.1:+27762917 | MS.gene68407:CDS |
| AGTGAAGCCTAGACCAGTGC+AGG | 0.532479 | 1.1:+27756500 | MS.gene68407:CDS |
| AGCGTGAAGTTTGTAGGTCA+GGG | 0.533196 | 1.1:+27760187 | MS.gene68407:CDS |
| AAAAGGAACGAGACGAAGCT+AGG | 0.533326 | 1.1:+27759124 | MS.gene68407:CDS |
| GTTCCAAGAATAAGACCATC+GGG | 0.534047 | 1.1:-27762744 | None:intergenic |
| TAACTGCAAACAGATCTTGA+AGG | 0.534904 | 1.1:+27760698 | MS.gene68407:CDS |
| GTTCATAGCAATGGGAAAAG+AGG | 0.541436 | 1.1:+27759216 | MS.gene68407:CDS |
| TTGCAAATGTGAAATCTGAA+TGG | 0.541920 | 1.1:+27763862 | MS.gene68407:CDS |
| GTAGCTAATACTTGTCCTGA+TGG | 0.546408 | 1.1:-27760057 | None:intergenic |
| TTGTGGCAAGGGTCAGATGA+TGG | 0.550255 | 1.1:+27760669 | MS.gene68407:CDS |
| TCAACAGAAGCCAAAGTCGC+AGG | 0.550956 | 1.1:-27759705 | None:intergenic |
| TTCAGGAACTTGTCTGACTC+AGG | 0.555913 | 1.1:+27762446 | MS.gene68407:CDS |
| AGCTAGGTCTATACTTGCAC+AGG | 0.561984 | 1.1:+27759140 | MS.gene68407:CDS |
| TAGGTTTCTGACTCTTCCGA+AGG | 0.569370 | 1.1:+27762699 | MS.gene68407:intron |
| CTATACTTGCACAGGCAGAA+AGG | 0.575588 | 1.1:+27759148 | MS.gene68407:CDS |
| TCAGCAACTCCAACAAACTC+TGG | 0.577891 | 1.1:+27763174 | MS.gene68407:CDS |
| AGGCTCATCTGGAACCTCAC+CGG | 0.580055 | 1.1:-27753878 | None:intergenic |
| AGAGCCTGCAACTGCAAGGT+AGG | 0.584256 | 1.1:-27763315 | None:intergenic |
| GTTAAGTCATGCTCTATACC+AGG | 0.586754 | 1.1:+27756846 | MS.gene68407:CDS |
| AATGCGAAGAAATTACATCC+TGG | 0.591581 | 1.1:+27759438 | MS.gene68407:CDS |
| AGTCACAAAGTAATTATTGG+TGG | 0.592605 | 1.1:-27762385 | None:intergenic |
| GCTAAGTTTGATGGACATGT+CGG | 0.592762 | 1.1:+27762916 | MS.gene68407:CDS |
| AGTTCCAAGAATAAGACCAT+CGG | 0.599921 | 1.1:-27762745 | None:intergenic |
| CTAATACTTGTCCTGATGGA+CGG | 0.602167 | 1.1:-27760053 | None:intergenic |
| CATCAGAGCCTGCAACTGCA+AGG | 0.620846 | 1.1:-27763319 | None:intergenic |
| TCGAGAAGAGATTAATCGAG+AGG | 0.622459 | 1.1:+27753929 | MS.gene68407:CDS |
| ACAATATCATCCAGAGTAAG+CGG | 0.631161 | 1.1:-27756310 | None:intergenic |
| AGCAGCAGTTGCATACAGCA+AGG | 0.634251 | 1.1:+27756818 | MS.gene68407:CDS |
| TGATAGACCGCAGCTCATGA+TGG | 0.652388 | 1.1:+27763093 | MS.gene68407:intron |
| TGTGCCGATCAAAACAGGCA+AGG | 0.675176 | 1.1:+27756330 | MS.gene68407:CDS |
| TTAAACAAAACTAGCAAACA+GGG | 0.680512 | 1.1:+27759765 | MS.gene68407:CDS |
| CTTTACACCATCATGAGCTG+CGG | 0.684333 | 1.1:-27763100 | None:intergenic |
| GCAGTGGAGTTTGACCACAG+TGG | 0.700459 | 1.1:+27763290 | MS.gene68407:intron |
| TGAGGTTCCAGATGAGCCTG+TGG | 0.703744 | 1.1:+27753882 | MS.gene68407:CDS |
| TGAGAATGGATACTACCTTG+CGG | 0.717359 | 1.1:+27762963 | MS.gene68407:CDS |
| CAAGTATTAGCTACACTCAG+CGG | 0.734442 | 1.1:+27760066 | MS.gene68407:CDS |
| CTGCAAGGTAGGATCCACTG+TGG | 0.800585 | 1.1:-27763304 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 27753875 | 27763921 | 27753875 | ID=MS.gene68407 |
| chr1.1 | mRNA | 27753875 | 27763921 | 27753875 | ID=MS.gene68407.t1;Parent=MS.gene68407 |
| chr1.1 | exon | 27753875 | 27753960 | 27753875 | ID=MS.gene68407.t1.exon1;Parent=MS.gene68407.t1 |
| chr1.1 | CDS | 27753875 | 27753960 | 27753875 | ID=cds.MS.gene68407.t1;Parent=MS.gene68407.t1 |
| chr1.1 | exon | 27756280 | 27756351 | 27756280 | ID=MS.gene68407.t1.exon2;Parent=MS.gene68407.t1 |
| chr1.1 | CDS | 27756280 | 27756351 | 27756280 | ID=cds.MS.gene68407.t1;Parent=MS.gene68407.t1 |
| chr1.1 | exon | 27756498 | 27756560 | 27756498 | ID=MS.gene68407.t1.exon3;Parent=MS.gene68407.t1 |
| chr1.1 | CDS | 27756498 | 27756560 | 27756498 | ID=cds.MS.gene68407.t1;Parent=MS.gene68407.t1 |
| chr1.1 | exon | 27756781 | 27756867 | 27756781 | ID=MS.gene68407.t1.exon4;Parent=MS.gene68407.t1 |
| chr1.1 | CDS | 27756781 | 27756867 | 27756781 | ID=cds.MS.gene68407.t1;Parent=MS.gene68407.t1 |
| chr1.1 | exon | 27759090 | 27759237 | 27759090 | ID=MS.gene68407.t1.exon5;Parent=MS.gene68407.t1 |
| chr1.1 | CDS | 27759090 | 27759237 | 27759090 | ID=cds.MS.gene68407.t1;Parent=MS.gene68407.t1 |
| chr1.1 | exon | 27759412 | 27759533 | 27759412 | ID=MS.gene68407.t1.exon6;Parent=MS.gene68407.t1 |
| chr1.1 | CDS | 27759412 | 27759533 | 27759412 | ID=cds.MS.gene68407.t1;Parent=MS.gene68407.t1 |
| chr1.1 | exon | 27759702 | 27759818 | 27759702 | ID=MS.gene68407.t1.exon7;Parent=MS.gene68407.t1 |
| chr1.1 | CDS | 27759702 | 27759818 | 27759702 | ID=cds.MS.gene68407.t1;Parent=MS.gene68407.t1 |
| chr1.1 | exon | 27760006 | 27760101 | 27760006 | ID=MS.gene68407.t1.exon8;Parent=MS.gene68407.t1 |
| chr1.1 | CDS | 27760006 | 27760101 | 27760006 | ID=cds.MS.gene68407.t1;Parent=MS.gene68407.t1 |
| chr1.1 | exon | 27760181 | 27760240 | 27760181 | ID=MS.gene68407.t1.exon9;Parent=MS.gene68407.t1 |
| chr1.1 | CDS | 27760181 | 27760240 | 27760181 | ID=cds.MS.gene68407.t1;Parent=MS.gene68407.t1 |
| chr1.1 | exon | 27760660 | 27760734 | 27760660 | ID=MS.gene68407.t1.exon10;Parent=MS.gene68407.t1 |
| chr1.1 | CDS | 27760660 | 27760734 | 27760660 | ID=cds.MS.gene68407.t1;Parent=MS.gene68407.t1 |
| chr1.1 | exon | 27762363 | 27762467 | 27762363 | ID=MS.gene68407.t1.exon11;Parent=MS.gene68407.t1 |
| chr1.1 | CDS | 27762363 | 27762467 | 27762363 | ID=cds.MS.gene68407.t1;Parent=MS.gene68407.t1 |
| chr1.1 | exon | 27762702 | 27762812 | 27762702 | ID=MS.gene68407.t1.exon12;Parent=MS.gene68407.t1 |
| chr1.1 | CDS | 27762702 | 27762812 | 27762702 | ID=cds.MS.gene68407.t1;Parent=MS.gene68407.t1 |
| chr1.1 | exon | 27762907 | 27762984 | 27762907 | ID=MS.gene68407.t1.exon13;Parent=MS.gene68407.t1 |
| chr1.1 | CDS | 27762907 | 27762984 | 27762907 | ID=cds.MS.gene68407.t1;Parent=MS.gene68407.t1 |
| chr1.1 | exon | 27763099 | 27763195 | 27763099 | ID=MS.gene68407.t1.exon14;Parent=MS.gene68407.t1 |
| chr1.1 | CDS | 27763099 | 27763195 | 27763099 | ID=cds.MS.gene68407.t1;Parent=MS.gene68407.t1 |
| chr1.1 | exon | 27763294 | 27763345 | 27763294 | ID=MS.gene68407.t1.exon15;Parent=MS.gene68407.t1 |
| chr1.1 | CDS | 27763294 | 27763345 | 27763294 | ID=cds.MS.gene68407.t1;Parent=MS.gene68407.t1 |
| chr1.1 | exon | 27763851 | 27763921 | 27763851 | ID=MS.gene68407.t1.exon16;Parent=MS.gene68407.t1 |
| chr1.1 | CDS | 27763851 | 27763921 | 27763851 | ID=cds.MS.gene68407.t1;Parent=MS.gene68407.t1 |
| Gene Sequence |
| Protein sequence |
