AlfalfaGEDB | MS.gene68636

M. sativa cultivar XinJiangDaYe / MS.gene68636

KEGG: K18633
GO: GO:0000930 GO:0000931 GO:0005575 GO:0008104 GO:0008150 GO:0008274 GO:0031503 GO:0032991 GO:0033036 GO:0033566 GO:0034613 GO:0034629 GO:0051179 GO:0051641 GO:0070727
PFAM: PF12554.11

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68636.t1	KEH36435.1	98.6	70	1	0	1	70	37	106	2.40E-29	137.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68636.t1	Q9M0N8	73.9	69	17	1	1	69	1	68	8.0e-21	100.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68636.t1	A0A072VE60	98.6	70	1	0	1	70	37	106	1.8e-29	137.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene060137	MS.gene68636	0.812545	4.02E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68636.t1	MTR_2g008230	98.571	70	1	0	1	70	37	106	2.18e-45	140
MS.gene68636.t1	MTR_4g124060	97.143	70	2	0	1	70	1	70	1.74e-43	135

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68636.t1	AT4G09550	73.913	69	17	1	1	69	1	68	1.51e-31	104
MS.gene68636.t1	AT4G09550	73.913	69	17	1	1	69	1	68	1.51e-31	104
MS.gene68636.t1	AT1G73790	72.414	58	16	0	1	58	1	58	3.63e-27	93.6

Find 23 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGTGCACTTCTTGCAGCTTC+AGG	0.145529	8.1:+7370563	None:intergenic
ACAAAATACATTCATGATTT+AGG	0.172021	8.1:+7370362	None:intergenic
CTCATTGCCCTCTGTGATCT+TGG	0.190398	8.1:-7370467	MS.gene68636:CDS
TCATTGCCCTCTGTGATCTT+GGG	0.281322	8.1:-7370466	MS.gene68636:CDS
ACAATAGCAGCAAGTGCTTC+AGG	0.324395	8.1:+7370437	None:intergenic
TAAACCCGTGTCGAGTATAT+TGG	0.366888	8.1:+7370511	None:intergenic
AAGAAGTGCACGAGAATCTT+TGG	0.388040	8.1:-7370552	MS.gene68636:CDS
GTGCACTTCTTGCAGCTTCA+GGG	0.392834	8.1:+7370564	None:intergenic
CATTGCCCTCTGTGATCTTG+GGG	0.494922	8.1:-7370465	MS.gene68636:CDS
TAGGATGCAATGATGACATT+GGG	0.500215	8.1:+7370390	None:intergenic
TATTGTCAAGGAACTTAGAA+AGG	0.527792	8.1:-7370420	MS.gene68636:CDS
ATTCATGATTTAGGAGGCGT+AGG	0.534552	8.1:+7370371	None:intergenic
AAATACATTCATGATTTAGG+AGG	0.583464	8.1:+7370365	None:intergenic
TATTGGACATGTGAAATGCC+AGG	0.592806	8.1:+7370528	None:intergenic
GTAGGATGCAATGATGACAT+TGG	0.595456	8.1:+7370389	None:intergenic
TGCACGAGAATCTTTGGACC+TGG	0.595713	8.1:-7370546	MS.gene68636:CDS
CATGTCCAATATACTCGACA+CGG	0.596616	8.1:-7370516	MS.gene68636:CDS
AGCACTTGCTGCTATTGTCA+AGG	0.625051	8.1:-7370432	MS.gene68636:CDS
TCAAGGAACTTAGAAAGGAG+AGG	0.641589	8.1:-7370415	MS.gene68636:CDS
ATTGACCCCAAGATCACAGA+GGG	0.646842	8.1:+7370460	None:intergenic
TGAGAATAGAAAGTGCATGA+CGG	0.680398	8.1:+7370486	None:intergenic
ATGTCCAATATACTCGACAC+GGG	0.686297	8.1:-7370515	MS.gene68636:CDS
GATTGACCCCAAGATCACAG+AGG	0.775929	8.1:+7370459	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TATTGTCAAGGAACTTAGAA+AGG	-	chr8.1:7370522-7370541	MS.gene68636:CDS	30.0%
	TAGGATGCAATGATGACATT+GGG	+	chr8.1:7370555-7370574	None:intergenic	35.0%
!	TGAGAATAGAAAGTGCATGA+CGG	+	chr8.1:7370459-7370478	None:intergenic	35.0%
	ATGTCCAATATACTCGACAC+GGG	-	chr8.1:7370427-7370446	MS.gene68636:CDS	40.0%
	CATGTCCAATATACTCGACA+CGG	-	chr8.1:7370426-7370445	MS.gene68636:CDS	40.0%
	GTAGGATGCAATGATGACAT+TGG	+	chr8.1:7370556-7370575	None:intergenic	40.0%
	TAAACCCGTGTCGAGTATAT+TGG	+	chr8.1:7370434-7370453	None:intergenic	40.0%
	TATTGGACATGTGAAATGCC+AGG	+	chr8.1:7370417-7370436	None:intergenic	40.0%
	TCAAGGAACTTAGAAAGGAG+AGG	-	chr8.1:7370527-7370546	MS.gene68636:CDS	40.0%
!	AAGAAGTGCACGAGAATCTT+TGG	-	chr8.1:7370390-7370409	MS.gene68636:CDS	40.0%
	ACAATAGCAGCAAGTGCTTC+AGG	+	chr8.1:7370508-7370527	None:intergenic	45.0%
	ATTGACCCCAAGATCACAGA+GGG	+	chr8.1:7370485-7370504	None:intergenic	45.0%
	TCATTGCCCTCTGTGATCTT+GGG	-	chr8.1:7370476-7370495	MS.gene68636:CDS	45.0%
!	AGCACTTGCTGCTATTGTCA+AGG	-	chr8.1:7370510-7370529	MS.gene68636:CDS	45.0%
	CATTGCCCTCTGTGATCTTG+GGG	-	chr8.1:7370477-7370496	MS.gene68636:CDS	50.0%
	CTCATTGCCCTCTGTGATCT+TGG	-	chr8.1:7370475-7370494	MS.gene68636:CDS	50.0%
	GATTGACCCCAAGATCACAG+AGG	+	chr8.1:7370486-7370505	None:intergenic	50.0%
	GTGCACTTCTTGCAGCTTCA+GGG	+	chr8.1:7370381-7370400	None:intergenic	50.0%
	TGCACGAGAATCTTTGGACC+TGG	-	chr8.1:7370396-7370415	MS.gene68636:CDS	50.0%
	CGTGCACTTCTTGCAGCTTC+AGG	+	chr8.1:7370382-7370401	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	7370373	7370591	7370373	ID=MS.gene68636
chr8.1	mRNA	7370373	7370591	7370373	ID=MS.gene68636.t1;Parent=MS.gene68636
chr8.1	exon	7370373	7370591	7370373	ID=MS.gene68636.t1.exon1;Parent=MS.gene68636.t1
chr8.1	CDS	7370373	7370591	7370373	ID=cds.MS.gene68636.t1;Parent=MS.gene68636.t1

Gene Sequence	>MS.gene68636 ATGGACCCTGAAGCTGCAAGAAGTGCACGAGAATCTTTGGACCTGGCATTTCACATGTCCAATATACTCGACACGGGTTTAGACCGTCATGCACTTTCTATTCTCATTGCCCTCTGTGATCTTGGGGTCAATCCTGAAGCACTTGCTGCTATTGTCAAGGAACTTAGAAAGGAGAGGCTCCCAATGTCATCATTGCATCCTACGCCTCCTAAATCATGA
Protein sequence	>MS.gene68636.t1 MDPEAARSARESLDLAFHMSNILDTGLDRHALSILIALCDLGVNPEALAAIVKELRKERLPMSSLHPTPPKS