AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68646

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68646.t1 MTR_2g093740 93.964 613 36 1 1 612 198 810 0.0 1202
MS.gene68646.t1 MTR_2g093740 93.964 613 36 1 1 612 68 680 0.0 1201
MS.gene68646.t1 MTR_2g093740 93.964 613 36 1 1 612 211 823 0.0 1200
MS.gene68646.t1 MTR_2g093740 93.964 613 36 1 1 612 81 693 0.0 1198
MS.gene68646.t1 MTR_2g093740 93.852 553 33 1 1 552 198 750 0.0 1084
MS.gene68646.t1 MTR_2g093740 93.852 553 33 1 1 552 68 620 0.0 1083
MS.gene68646.t1 MTR_2g093740 93.852 553 33 1 1 552 211 763 0.0 1082
MS.gene68646.t1 MTR_2g093740 93.852 553 33 1 1 552 81 633 0.0 1081
MS.gene68646.t1 MTR_2g093740 93.469 490 31 1 1 489 198 687 0.0 955
MS.gene68646.t1 MTR_2g093740 93.469 490 31 1 1 489 211 700 0.0 954
MS.gene68646.t1 MTR_4g060460 71.961 617 143 10 1 612 196 787 0.0 883
MS.gene68646.t1 MTR_4g060460 71.498 621 144 10 1 612 196 792 0.0 882
MS.gene68646.t1 MTR_4g088210 37.654 486 268 11 1 469 178 645 2.51e-89 291
MS.gene68646.t1 MTR_4g088210 37.810 484 270 11 1 469 178 645 3.08e-89 291
MS.gene68646.t1 MTR_2g014770 48.060 335 155 9 1 326 188 512 5.96e-89 290
MS.gene68646.t1 MTR_2g014770 54.651 258 113 4 1 257 188 442 2.40e-84 276
MS.gene68646.t1 MTR_8g100050 48.029 279 140 4 1 275 192 469 7.51e-80 267
MS.gene68646.t1 MTR_8g100050 48.029 279 140 4 1 275 192 469 5.79e-79 267
MS.gene68646.t1 MTR_8g100050 50.000 104 52 0 479 582 705 808 1.47e-30 128
MS.gene68646.t1 MTR_2g005240 52.489 221 104 1 1 221 155 374 1.10e-70 241
MS.gene68646.t1 MTR_2g005240 46.212 132 67 2 474 601 537 668 3.10e-28 120
MS.gene68646.t1 MTR_5g076270 51.442 208 99 2 1 207 158 364 6.80e-64 226
MS.gene68646.t1 MTR_5g076270 36.691 139 80 4 437 572 670 803 4.59e-19 92.0
MS.gene68646.t1 MTR_8g079492 49.545 220 109 2 1 219 74 292 1.66e-63 224
MS.gene68646.t1 MTR_8g079492 38.095 126 74 3 450 572 663 787 1.23e-19 94.0
MS.gene68646.t1 MTR_8g079492 49.545 220 109 2 1 219 156 374 5.65e-63 224
MS.gene68646.t1 MTR_8g079492 33.333 150 96 3 426 572 721 869 1.32e-19 94.0
MS.gene68646.t1 MTR_8g079492 49.545 220 109 2 1 219 156 374 5.81e-63 224
MS.gene68646.t1 MTR_8g079492 33.333 150 96 3 426 572 722 870 1.35e-19 94.0
MS.gene68646.t1 MTR_8g027440 49.528 212 102 4 1 211 161 368 6.83e-63 220
MS.gene68646.t1 MTR_8g027440 51.579 95 45 1 484 578 558 651 3.66e-24 107
MS.gene68646.t1 MTR_3g064050 51.923 208 98 2 1 207 160 366 8.56e-63 223
MS.gene68646.t1 MTR_3g064050 41.880 117 59 3 464 572 696 811 5.55e-19 91.7
MS.gene68646.t1 MTR_2g018690 49.545 220 109 2 1 219 157 375 1.35e-62 223
MS.gene68646.t1 MTR_2g018690 40.152 132 73 4 451 578 743 872 6.09e-21 98.2
MS.gene68646.t1 MTR_7g062540 44.672 244 129 5 1 242 146 385 8.41e-62 217
MS.gene68646.t1 MTR_7g062540 48.148 108 52 2 468 575 557 660 2.13e-24 108
MS.gene68646.t1 MTR_4g021580 50.485 206 98 3 1 205 151 353 1.31e-61 215
MS.gene68646.t1 MTR_4g021580 47.619 84 40 2 468 551 542 621 1.60e-15 80.5
MS.gene68646.t1 MTR_4g021580 50.485 206 98 3 1 205 151 353 1.97e-61 216
MS.gene68646.t1 MTR_4g021580 47.222 108 53 2 468 575 542 645 2.87e-22 101
MS.gene68646.t1 MTR_1g024025 50.242 207 101 2 1 206 176 381 5.31e-57 208
MS.gene68646.t1 MTR_1g024025 46.154 91 47 2 484 573 754 843 2.64e-18 90.1
MS.gene68646.t1 MTR_4g124900 50.495 202 99 1 1 202 156 356 8.25e-55 201
MS.gene68646.t1 MTR_4g124900 49.485 97 47 2 479 574 965 1060 1.16e-22 103
MS.gene68646.t1 MTR_4g124900 50.495 202 99 1 1 202 156 356 1.00e-54 201
MS.gene68646.t1 MTR_4g124900 49.485 97 47 2 479 574 988 1083 1.36e-22 103
MS.gene68646.t1 MTR_2g043250 49.010 202 102 1 1 202 156 356 4.04e-54 199
MS.gene68646.t1 MTR_2g043250 49.485 97 47 2 479 574 981 1076 1.07e-21 100
MS.gene68646.t1 MTR_8g101360 50.485 206 100 2 1 205 165 369 2.13e-52 194
MS.gene68646.t1 MTR_8g101360 48.913 92 45 2 484 574 984 1074 5.37e-21 98.6
MS.gene68646.t1 MTR_8g446900 41.546 207 113 3 1 205 92 292 2.20e-49 176
MS.gene68646.t1 MTR_1g064430 37.591 274 121 6 1 231 159 425 9.15e-45 169
MS.gene68646.t1 MTR_1g064430 37.591 274 121 6 1 231 172 438 1.02e-44 169
MS.gene68646.t1 MTR_2g094570 39.916 238 106 5 1 201 138 375 1.89e-43 166
MS.gene68646.t1 MTR_2g094570 39.916 238 106 5 1 201 194 431 2.47e-43 166
MS.gene68646.t1 MTR_2g094570 39.916 238 106 5 1 201 138 375 2.52e-43 166
MS.gene68646.t1 MTR_4g058930 38.583 254 113 4 1 211 139 392 4.04e-42 162
MS.gene68646.t1 MTR_4g058930 38.583 254 113 4 1 211 139 392 4.92e-42 162
MS.gene68646.t1 MTR_1g094960 36.992 246 119 3 1 211 148 392 1.31e-41 159
MS.gene68646.t1 MTR_3g073420 35.743 249 120 5 1 209 147 395 2.08e-34 138
MS.gene68646.t1 MTR_7g101280 33.992 253 120 4 1 207 161 412 2.29e-34 139
MS.gene68646.t1 MTR_0041s0110 33.992 253 120 4 1 207 161 412 2.29e-34 139
MS.gene68646.t1 MTR_7g101275 41.209 182 100 4 1 179 122 299 4.49e-32 129
MS.gene68646.t1 MTR_5g040880 32.143 224 132 6 1 219 143 351 2.19e-29 122
MS.gene68646.t1 MTR_2g024430 32.407 216 126 6 1 212 152 351 3.64e-28 119
MS.gene68646.t1 MTR_5g061220 30.522 249 135 6 1 218 148 389 7.48e-28 118
MS.gene68646.t1 MTR_3g064050 43.077 130 72 2 79 207 1 129 5.48e-25 110
MS.gene68646.t1 MTR_3g064050 41.880 117 59 3 464 572 459 574 4.36e-19 91.7
MS.gene68646.t1 MTR_5g082140 32.751 229 133 7 1 223 144 357 9.57e-25 108
MS.gene68646.t1 MTR_5g040740 33.632 223 112 11 1 213 143 339 8.61e-23 101
MS.gene68646.t1 MTR_1g058210 28.384 229 133 5 8 223 150 360 2.90e-22 100
MS.gene68646.t1 MTR_0041s0120 33.500 200 102 4 1 179 122 311 4.50e-22 99.4
MS.gene68646.t1 MTR_5g074840 47.253 91 48 0 33 123 58 148 5.57e-19 84.7
MS.gene68646.t1 MTR_2g006270 26.860 242 163 5 1 240 146 375 1.48e-17 86.3
MS.gene68646.t1 MTR_2g006380 30.909 220 123 9 8 222 160 355 7.21e-17 84.0
MS.gene68646.t1 MTR_5g060630 29.730 222 139 9 1 211 169 384 7.71e-17 84.3
MS.gene68646.t1 MTR_5g460740 29.730 222 139 9 1 211 169 384 7.71e-17 84.3
MS.gene68646.t1 MTR_5g032260 34.921 126 76 3 76 200 34 154 2.49e-15 79.0
MS.gene68646.t1 MTR_5g060780 28.502 207 134 6 8 205 144 345 1.08e-13 73.6
MS.gene68646.t1 MTR_5g460920 28.502 207 134 6 8 205 144 345 1.23e-13 73.6
MS.gene68646.t1 MTR_5g062970 44.262 61 34 0 7 67 174 234 3.11e-12 67.4
MS.gene68646.t1 MTR_5g061890 29.327 208 131 8 8 205 144 345 9.01e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68646.t1 AT5G60450 55.412 619 232 12 1 612 183 764 0.0 623
MS.gene68646.t1 AT5G60450 55.412 619 232 12 1 612 207 788 0.0 622
MS.gene68646.t1 AT5G62000 34.808 678 355 17 1 609 194 853 1.41e-117 370
MS.gene68646.t1 AT5G62000 34.808 678 355 17 1 609 194 853 1.41e-117 370
MS.gene68646.t1 AT5G62000 34.808 678 355 17 1 609 194 853 1.41e-117 370
MS.gene68646.t1 AT5G62000 34.808 678 355 17 1 609 194 853 1.41e-117 370
MS.gene68646.t1 AT5G62000 35.703 647 334 16 1 578 194 827 2.24e-117 369
MS.gene68646.t1 AT2G33860 45.482 332 151 5 1 332 32 333 7.06e-88 280
MS.gene68646.t1 AT2G33860 45.482 332 151 5 1 332 189 490 3.48e-86 281
MS.gene68646.t1 AT3G61830 45.607 239 120 4 1 233 107 341 2.02e-69 235
MS.gene68646.t1 AT3G61830 35.519 183 78 5 433 576 348 529 1.26e-24 108
MS.gene68646.t1 AT3G61830 45.607 239 120 4 1 233 158 392 7.77e-69 234
MS.gene68646.t1 AT3G61830 49.020 102 51 1 475 576 480 580 1.66e-24 108
MS.gene68646.t1 AT2G46530 46.188 223 114 4 1 221 67 285 2.15e-65 223
MS.gene68646.t1 AT2G46530 44.000 125 65 2 448 572 371 490 8.71e-26 112
MS.gene68646.t1 AT2G46530 46.188 223 114 4 1 221 154 372 1.56e-64 223
MS.gene68646.t1 AT2G46530 44.000 125 65 2 448 572 458 577 1.86e-25 111
MS.gene68646.t1 AT2G46530 46.188 223 114 4 1 221 175 393 2.67e-64 223
MS.gene68646.t1 AT2G46530 44.000 125 65 2 448 572 479 598 2.46e-25 111
MS.gene68646.t1 AT1G59750 47.982 223 113 2 1 221 154 375 7.82e-63 219
MS.gene68646.t1 AT1G59750 56.000 100 44 0 474 573 527 626 1.21e-29 124
MS.gene68646.t1 AT1G59750 47.982 223 113 2 1 221 154 375 8.87e-63 219
MS.gene68646.t1 AT1G59750 56.000 100 44 0 474 573 529 628 1.19e-29 124
MS.gene68646.t1 AT1G59750 47.982 223 113 2 1 221 154 375 9.24e-63 219
MS.gene68646.t1 AT1G59750 56.000 100 44 0 474 573 532 631 1.15e-29 124
MS.gene68646.t1 AT1G59750 47.982 223 113 2 1 221 154 375 9.24e-63 219
MS.gene68646.t1 AT1G59750 56.000 100 44 0 474 573 532 631 1.15e-29 124
MS.gene68646.t1 AT5G37020 49.545 220 109 2 1 219 61 279 2.16e-62 219
MS.gene68646.t1 AT5G37020 47.778 90 45 2 484 572 610 698 1.34e-19 93.6
MS.gene68646.t1 AT5G37020 50.962 208 100 2 1 207 156 362 4.54e-62 219
MS.gene68646.t1 AT5G37020 52.174 69 31 2 484 551 705 772 2.35e-16 83.6
MS.gene68646.t1 AT5G37020 50.962 208 100 2 1 207 156 362 8.27e-62 219
MS.gene68646.t1 AT5G37020 47.778 90 45 2 484 572 705 793 1.89e-19 93.2
MS.gene68646.t1 AT4G23980 51.485 202 94 3 1 202 146 343 9.18e-62 216
MS.gene68646.t1 AT4G23980 53.000 100 46 1 476 575 515 613 2.05e-26 114
MS.gene68646.t1 AT4G23980 51.485 202 94 3 1 202 146 343 1.01e-61 216
MS.gene68646.t1 AT4G23980 53.000 100 46 1 476 575 513 611 2.11e-26 114
MS.gene68646.t1 AT1G30330 46.311 244 115 3 1 229 159 401 1.02e-61 221
MS.gene68646.t1 AT1G30330 46.078 102 51 3 474 572 784 884 5.38e-21 98.2
MS.gene68646.t1 AT1G30330 46.311 244 115 3 1 229 159 401 1.02e-61 221
MS.gene68646.t1 AT1G30330 46.078 102 51 3 474 572 784 884 5.38e-21 98.2
MS.gene68646.t1 AT1G30330 46.311 244 115 3 1 229 157 399 1.06e-61 221
MS.gene68646.t1 AT1G30330 46.078 102 51 3 474 572 782 882 5.61e-21 98.2
MS.gene68646.t1 AT1G19850 49.758 207 102 2 1 206 188 393 2.83e-57 207
MS.gene68646.t1 AT1G19850 45.455 99 52 2 476 573 785 882 2.97e-19 92.8
MS.gene68646.t1 AT1G19850 49.758 207 102 2 1 206 177 382 2.93e-57 207
MS.gene68646.t1 AT1G19850 45.455 99 52 2 476 573 774 871 2.97e-19 92.8
MS.gene68646.t1 AT1G19220 50.495 202 99 1 1 202 156 356 1.86e-54 201
MS.gene68646.t1 AT1G19220 46.903 113 54 3 479 586 953 1064 2.98e-23 105
MS.gene68646.t1 AT1G35540 44.595 222 117 4 1 222 156 371 3.37e-54 195
MS.gene68646.t1 AT1G35540 30.268 261 126 7 318 572 387 597 1.38e-24 108
MS.gene68646.t1 AT1G34310 43.049 223 120 4 1 220 156 374 5.19e-54 194
MS.gene68646.t1 AT1G34310 48.624 109 54 2 456 563 482 589 4.12e-23 104
MS.gene68646.t1 AT5G20730 50.000 202 100 1 1 202 157 357 9.74e-54 198
MS.gene68646.t1 AT5G20730 38.182 165 78 6 413 574 984 1127 4.27e-22 102
MS.gene68646.t1 AT1G35520 44.344 221 117 4 1 221 156 370 5.72e-53 191
MS.gene68646.t1 AT1G35520 46.789 109 56 2 456 563 482 589 4.45e-22 100
MS.gene68646.t1 AT1G35240 43.891 221 118 4 1 221 156 370 5.92e-53 191
MS.gene68646.t1 AT1G35240 52.632 95 44 1 476 570 489 582 1.79e-23 105
MS.gene68646.t1 AT1G34410 41.704 223 123 4 1 220 156 374 3.46e-52 189
MS.gene68646.t1 AT1G34410 53.684 95 43 1 476 570 503 596 6.53e-24 106
MS.gene68646.t1 AT5G20730 49.754 203 100 2 1 202 157 358 3.74e-52 194
MS.gene68646.t1 AT5G20730 38.182 165 78 6 413 574 985 1128 4.36e-22 102
MS.gene68646.t1 AT5G20730 49.754 203 100 2 1 202 157 358 4.28e-52 194
MS.gene68646.t1 AT5G20730 38.182 165 78 6 413 574 985 1128 4.39e-22 102
MS.gene68646.t1 AT1G34390 44.068 236 123 6 1 233 154 383 1.38e-51 187
MS.gene68646.t1 AT1G34390 46.364 110 57 2 456 564 480 588 2.94e-21 98.6
MS.gene68646.t1 AT1G34170 41.228 228 125 5 1 226 154 374 1.22e-49 180
MS.gene68646.t1 AT2G28350 36.296 270 130 3 1 231 145 411 1.55e-44 169
MS.gene68646.t1 AT4G30080 39.271 247 115 4 1 213 150 395 1.64e-44 169
MS.gene68646.t1 AT2G28350 36.296 270 130 3 1 231 145 411 2.08e-44 169
MS.gene68646.t1 AT1G34170 37.719 228 107 5 1 226 154 348 7.82e-39 149
MS.gene68646.t1 AT1G34170 37.719 228 107 5 1 226 154 348 2.46e-38 149
MS.gene68646.t1 AT1G34170 40.566 106 55 2 476 574 418 522 1.17e-18 90.1
MS.gene68646.t1 AT1G77850 26.979 341 199 7 1 305 149 475 1.58e-30 127
MS.gene68646.t1 AT1G77850 26.979 341 199 7 1 305 149 475 1.94e-30 126
MS.gene68646.t1 AT1G43950 55.714 70 28 2 1 70 156 222 1.68e-17 82.4

Find 147 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTATAGCTGACCTTCTTTC+TGG 0.139715 2.1:+9175640 None:intergenic
GTAAATCCTTGTAGACTATT+TGG 0.158429 2.1:-9174364 MS.gene68646:CDS
CTTCTTCTTGTGTATATATA+TGG 0.165929 2.1:+9173544 None:intergenic
AGCCAAAAGAATCTTGTTTC+TGG 0.202166 2.1:-9176174 MS.gene68646:CDS
AACATTCTCTAGTTGGAAAT+TGG 0.212031 2.1:+9174549 None:intergenic
AGAAATGAAGTTGGAATATC+AGG 0.255023 2.1:-9174490 MS.gene68646:CDS
AGCTGGAGACAGTGGGTTTA+TGG 0.268758 2.1:-9175034 MS.gene68646:CDS
CCTGCAGCTTCTGCTTGTTT+CGG 0.298110 2.1:+9174458 None:intergenic
TTTGACTTGGGCCATTTATA+GGG 0.298149 2.1:+9175512 None:intergenic
CCCCAGAAACAAGATTCTTT+TGG 0.320379 2.1:+9176172 None:intergenic
AAACTTAACTTTCACTCATT+AGG 0.322689 2.1:-9174658 MS.gene68646:CDS
AGTGAACTAGAGAGACTATT+TGG 0.331029 2.1:-9174103 MS.gene68646:CDS
GTGATTTGGATCCCTATAAA+TGG 0.335220 2.1:-9175523 MS.gene68646:CDS
ATTTCCACTCCACACCATTC+AGG 0.344600 2.1:+9176348 None:intergenic
GAAGTGGAGAAGATGACAAT+TGG 0.348700 2.1:-9173525 MS.gene68646:CDS
CGGAAACGAACCGGTTTCCT+AGG 0.350274 2.1:-9174801 MS.gene68646:CDS
TAGTTGGAAATTGGTTGGTT+TGG 0.350673 2.1:+9174558 None:intergenic
TGGTTACCAACAATTGATTC+AGG 0.358579 2.1:+9175957 None:intergenic
TTCACCTACCTCGATAAATA+TGG 0.367329 2.1:+9176321 None:intergenic
CTGCAGTGAGTTCTGTGTTA+TGG 0.372350 2.1:-9173577 MS.gene68646:intron
ATTCAAAATGAAATTTGAAA+TGG 0.374517 2.1:-9175671 MS.gene68646:CDS
TTGATCTAAACCTTAACTCT+TGG 0.379579 2.1:-9174729 MS.gene68646:CDS
ATAAATGGCCCAAGTCAAAA+TGG 0.382489 2.1:-9175508 MS.gene68646:CDS
GCCAAAAGAATCTTGTTTCT+GGG 0.385570 2.1:-9176173 MS.gene68646:CDS
GGGACGCAGTGCTCTTTCTA+AGG 0.386238 2.1:-9176152 MS.gene68646:intron
TGATCTAAACCTTAACTCTT+GGG 0.391011 2.1:-9174728 MS.gene68646:CDS
TAAATGAAACATTCTCTAGT+TGG 0.401148 2.1:+9174542 None:intergenic
CACAAACAAGGGAGCTTAGT+TGG 0.402834 2.1:-9174172 MS.gene68646:CDS
TGACAGTGAAAATGACATTA+TGG 0.405346 2.1:-9174029 MS.gene68646:CDS
ATTTCTGCTGCTGCTTCTCT+AGG 0.415866 2.1:-9174430 MS.gene68646:CDS
TTGGTAAGGCACAACGAAAT+CGG 0.417321 2.1:+9175738 None:intergenic
CATACTAATCTTGCATCAAA+TGG 0.420961 2.1:-9174886 MS.gene68646:CDS
TAATGAGTGAAAGTTAAGTT+TGG 0.421167 2.1:+9174660 None:intergenic
GATTCAGGAAGACCGTTTCT+CGG 0.422054 2.1:+9175972 None:intergenic
TGATGATAACCAGAGTTGTT+TGG 0.426706 2.1:-9173493 MS.gene68646:CDS
GTCAAAATGGAGATGCTTGA+TGG 0.432058 2.1:-9175495 MS.gene68646:intron
AAAATGACATTATGGTTGTT+GGG 0.432406 2.1:-9174021 MS.gene68646:CDS
AAGTGGAGAAGATGACAATT+GGG 0.436343 2.1:-9173524 MS.gene68646:CDS
AATACTCCTTCAGCTCAGAT+AGG 0.438938 2.1:-9174520 MS.gene68646:CDS
TTCTAGTTATGCAGACTTCA+CGG 0.440375 2.1:-9174821 MS.gene68646:CDS
CAGACTTCACGGAAACGAAC+CGG 0.444346 2.1:-9174810 MS.gene68646:CDS
ACTACTGTTGGAGAAGAATC+TGG 0.448069 2.1:+9173420 None:intergenic
TTAGATCAACCTTTCCTGCC+AGG 0.450136 2.1:+9174743 None:intergenic
AGTGACTGGAATGAGTGATT+TGG 0.450736 2.1:-9175537 MS.gene68646:CDS
TTCTTGACCTTGCAAGACCT+TGG 0.450899 2.1:+9174987 None:intergenic
TCGACAAGATTGAATGTTCA+AGG 0.452440 2.1:+9175195 None:intergenic
ATCAAGATAGAGTCTCTCCT+TGG 0.455503 2.1:-9175246 MS.gene68646:CDS
GGTCTTCCTGAATCAATTGT+TGG 0.457519 2.1:-9175963 MS.gene68646:CDS
TTGGATTCATACCAGTGATA+AGG 0.459048 2.1:+9175125 None:intergenic
GTGAAGTCTGCATAACTAGA+AGG 0.460590 2.1:+9174823 None:intergenic
AAAAGTATGCTAAAAGCATC+AGG 0.463277 2.1:-9175717 MS.gene68646:CDS
TAAGTTTGGTTGCATTATGC+AGG 0.464035 2.1:+9174674 None:intergenic
GGTGGTTTCCGCCGCCGATA+AGG 0.472069 2.1:+9174336 None:intergenic
GTAGTGAGCAGATGCCTCCT+TGG 0.475837 2.1:+9176216 None:intergenic
TTCTCTAGTTGGAAATTGGT+TGG 0.476906 2.1:+9174553 None:intergenic
ACTTTGTGTATATCACCATA+TGG 0.477775 2.1:+9174607 None:intergenic
GAAAATGACATTATGGTTGT+TGG 0.484036 2.1:-9174022 MS.gene68646:CDS
CTTGCAAGACCCTAGGAAAC+CGG 0.485163 2.1:+9174791 None:intergenic
TTTGAAGCCTCCACAATCAA+TGG 0.488117 2.1:+9173462 None:intergenic
TCCTAGTTTCCTTTCTTCAG+TGG 0.491250 2.1:-9175925 MS.gene68646:CDS
TTTAGTAAAACTTACTGCCA+TGG 0.494050 2.1:+9173992 None:intergenic
AGGAGGCATCTGCTCACTAC+TGG 0.505121 2.1:-9176213 MS.gene68646:CDS
CATACCAGTGATAAGGTGAC+TGG 0.506398 2.1:+9175132 None:intergenic
GGCATCTGCTCACTACTGGC+TGG 0.509364 2.1:-9176209 MS.gene68646:CDS
TAAGAGATCCTGACAAAGGA+TGG 0.509914 2.1:-9174066 MS.gene68646:CDS
AACTAGATTCTAACTACTGT+TGG 0.511297 2.1:+9173408 None:intergenic
TATATCACCATATGGAAAAG+AGG 0.512064 2.1:+9174615 None:intergenic
ATCTTGTCCCTATGGTCAGT+GGG 0.513478 2.1:+9175692 None:intergenic
GTGATATTACACAGGTCCGA+TGG 0.515735 2.1:-9175291 MS.gene68646:intron
ATAATTTGTGTAACTAACAT+TGG 0.517422 2.1:+9174699 None:intergenic
AATCTTGTCCCTATGGTCAG+TGG 0.518063 2.1:+9175691 None:intergenic
ATTGAGCAGCTACAATGATC+TGG 0.518918 2.1:-9174128 MS.gene68646:CDS
TGAAAACGGAGAGTTAAGAT+TGG 0.519467 2.1:-9176015 MS.gene68646:CDS
AGAGCTGCTCGACCGAGAAA+CGG 0.520377 2.1:-9175984 MS.gene68646:CDS
CTTCTCTAGGTGCAAACTTG+AGG 0.522379 2.1:-9174417 MS.gene68646:CDS
ATTGAGATCCCTGGCAGGAA+AGG 0.523346 2.1:-9174752 MS.gene68646:CDS
TCTTGACCTTGCAAGACCCT+AGG 0.523482 2.1:+9174784 None:intergenic
GGTGCATTGAGAAATGAAGT+TGG 0.523787 2.1:-9174499 MS.gene68646:CDS
TATATATACACAAGAAGAAG+TGG 0.530321 2.1:-9173541 MS.gene68646:CDS
TCTCTATGTAGGGGTGAAAA+CGG 0.532577 2.1:-9176029 MS.gene68646:intron
TCAAGATAGAGTCTCTCCTT+GGG 0.533378 2.1:-9175245 MS.gene68646:CDS
TGAATGAAGGTTCACAAACA+AGG 0.534840 2.1:-9174184 MS.gene68646:intron
TCAGGAACCCACTGACCATA+GGG 0.535798 2.1:-9175699 MS.gene68646:CDS
TAGCATTAGCCACTGAAGAA+AGG 0.540509 2.1:+9175916 None:intergenic
CCGAAACAAGCAGAAGCTGC+AGG 0.542656 2.1:-9174458 MS.gene68646:CDS
TAGATCAACCTTTCCTGCCA+GGG 0.544208 2.1:+9174744 None:intergenic
TGTAGTAGCGGTATAGTGAC+TGG 0.545183 2.1:-9175551 MS.gene68646:CDS
TATGGTGATATACACAAAGT+TGG 0.548146 2.1:-9174604 MS.gene68646:CDS
GAAAACGGAGAGTTAAGATT+GGG 0.548310 2.1:-9176014 MS.gene68646:CDS
TGGATGAATCTCCAGAAAGA+AGG 0.548781 2.1:-9175651 MS.gene68646:intron
TTTCCTGCCAGGGATCTCAA+TGG 0.549270 2.1:+9174754 None:intergenic
GAGAGACTATTTGGCATGGA+AGG 0.550692 2.1:-9174094 MS.gene68646:CDS
GCTCAATCTTGAGAGATCAA+TGG 0.551608 2.1:+9174144 None:intergenic
AATATATCCATTGAGATCCC+TGG 0.553252 2.1:-9174761 MS.gene68646:CDS
GGAACATACACCATTGATTG+TGG 0.553625 2.1:-9173472 MS.gene68646:CDS
AGGCTGAAGAAACTGCGGAC+GGG 0.554562 2.1:-9175172 MS.gene68646:CDS
TACACAGGTCCGATGGGATG+AGG 0.555224 2.1:-9175284 MS.gene68646:intron
GGAAACTAGGAGAACAACTC+TGG 0.556326 2.1:+9175937 None:intergenic
TGAACATTCAATCTTGTCGA+AGG 0.558866 2.1:-9175192 MS.gene68646:CDS
AGGCTGAACCCTCGTAAACT+CGG 0.559216 2.1:+9174843 None:intergenic
GAAAATCCAAATAGTCTACA+AGG 0.566058 2.1:+9174358 None:intergenic
GCTTCAAAGTCTTCCTCAGT+GGG 0.567657 2.1:-9173447 MS.gene68646:CDS
CCAAAAGAATCTTGTTTCTG+GGG 0.569533 2.1:-9176172 MS.gene68646:CDS
TGATATTACACAGGTCCGAT+GGG 0.571325 2.1:-9175290 MS.gene68646:intron
CCGATGGGATGAGGATATTG+AGG 0.572780 2.1:-9175275 MS.gene68646:CDS
GCCACTGAAGAAAGGAAACT+AGG 0.575196 2.1:+9175924 None:intergenic
CGACAAGATTGAATGTTCAA+GGG 0.577787 2.1:+9175196 None:intergenic
GTGTATGTTCCAAACAACTC+TGG 0.578457 2.1:+9173484 None:intergenic
TGGTTGTTGGGGATGATCCA+TGG 0.581281 2.1:-9174009 MS.gene68646:CDS
TCACCCAGTCACCTTATCAC+TGG 0.584443 2.1:-9175136 MS.gene68646:intron
ATCAGGAACCCACTGACCAT+AGG 0.589785 2.1:-9175700 MS.gene68646:CDS
TATCCATTGAGATCCCTGGC+AGG 0.592828 2.1:-9174757 MS.gene68646:CDS
TTCATCGCAGCTGGAGACAG+TGG 0.594260 2.1:-9175042 MS.gene68646:intron
TCATCGCAGCTGGAGACAGT+GGG 0.597361 2.1:-9175041 MS.gene68646:intron
AATGCACCTATCTGAGCTGA+AGG 0.597701 2.1:+9174514 None:intergenic
CGGTTTCCTAGGGTCTTGCA+AGG 0.598452 2.1:-9174790 MS.gene68646:CDS
AATCTTTGTCAGGTCAGCCA+AGG 0.599067 2.1:-9176233 MS.gene68646:intron
AAGATTGAATGTTCAAGGGA+GGG 0.602810 2.1:+9175200 None:intergenic
TTACTTCTTCCGAGTTTACG+AGG 0.603395 2.1:-9174852 MS.gene68646:CDS
GATATACACAAAGTTGGTCA+AGG 0.608483 2.1:-9174598 MS.gene68646:CDS
GATCTAAACCTTAACTCTTG+GGG 0.609198 2.1:-9174727 MS.gene68646:CDS
AAATGACATTATGGTTGTTG+GGG 0.611574 2.1:-9174020 MS.gene68646:CDS
TTTGCAACAAGCTCTTGAGA+GGG 0.613596 2.1:+9176374 None:intergenic
GGCTTCAAAGTCTTCCTCAG+TGG 0.617874 2.1:-9173448 MS.gene68646:CDS
GGAAACGAACCGGTTTCCTA+GGG 0.618178 2.1:-9174800 MS.gene68646:CDS
AGATCGACCAAGGTCTTGCA+AGG 0.618905 2.1:-9174994 MS.gene68646:CDS
CCTCAATATCCTCATCCCAT+CGG 0.625293 2.1:+9175275 None:intergenic
CTTTGTCAGGTCAGCCAAGG+AGG 0.625815 2.1:-9176230 MS.gene68646:intron
GAATGAAGGTTCACAAACAA+GGG 0.629502 2.1:-9174183 MS.gene68646:intron
CAAGATTGAATGTTCAAGGG+AGG 0.630768 2.1:+9175199 None:intergenic
ACTAGAGAGACTATTTGGCA+TGG 0.631155 2.1:-9174098 MS.gene68646:CDS
TACTTCTTCCGAGTTTACGA+GGG 0.633533 2.1:-9174851 MS.gene68646:CDS
AAGGGAGGGAGAGAAGTAGA+CGG 0.635894 2.1:+9175214 None:intergenic
ACATACACCATTGATTGTGG+AGG 0.643124 2.1:-9173469 MS.gene68646:CDS
ATGTCTTGCAGGTGTAGTAG+CGG 0.648254 2.1:-9175563 MS.gene68646:intron
CTTCTAAGAGATCCTGACAA+AGG 0.649192 2.1:-9174070 MS.gene68646:CDS
CTTGTTGCAAAAGACCTGAA+TGG 0.653673 2.1:-9176362 MS.gene68646:CDS
AAGGCTGAAGAAACTGCGGA+CGG 0.656866 2.1:-9175173 MS.gene68646:CDS
TGCTAAAAGAAGTTGCACGA+AGG 0.662737 2.1:-9174293 MS.gene68646:intron
AAATTTCGCCATATTTATCG+AGG 0.663380 2.1:-9176329 MS.gene68646:intron
GTAGACGGATCGATCTCCCA+AGG 0.663808 2.1:+9175229 None:intergenic
AAGACCTGAATGGTGTGGAG+TGG 0.664698 2.1:-9176352 MS.gene68646:CDS
ATACCAGTGATAAGGTGACT+GGG 0.667650 2.1:+9175133 None:intergenic
GTCGAAGGCTGAAGAAACTG+CGG 0.672621 2.1:-9175177 MS.gene68646:CDS
TGCAAAAGACCTGAATGGTG+TGG 0.685027 2.1:-9176357 MS.gene68646:CDS
TGAGTCTATAAGATCGACCA+AGG 0.694503 2.1:-9175004 MS.gene68646:CDS
AGAATCTGGCTGTCCCACTG+AGG 0.716343 2.1:+9173434 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATTTATAATTATTTAA+TGG + chr2.1:9175885-9175904 None:intergenic 0.0%
!! AAACATATTAAATATACATG+TGG + chr2.1:9174015-9174034 None:intergenic 15.0%
!! AACATATTAAATATACATGT+GGG + chr2.1:9174014-9174033 None:intergenic 15.0%
!! ATTCAAAATGAAATTTGAAA+TGG - chr2.1:9174131-9174150 MS.gene68646:CDS 15.0%
!! TATTAATGTAATGTTTAGAA+AGG + chr2.1:9174447-9174466 None:intergenic 15.0%
!! TGATATTATATTTCAAATGT+CGG - chr2.1:9176109-9176128 MS.gene68646:intron 15.0%
!!! ATTTGAAATATAATATCACA+AGG + chr2.1:9176107-9176126 None:intergenic 15.0%
!! ACCATATGATATTTATTCAA+TGG + chr2.1:9176030-9176049 None:intergenic 20.0%
!! ACCATTGAATAAATATCATA+TGG - chr2.1:9176026-9176045 MS.gene68646:intron 20.0%
!! ATGTAATATACAATGAATGA+AGG - chr2.1:9175605-9175624 MS.gene68646:intron 20.0%
!! ATTCATATCACAATATAACT+CGG + chr2.1:9175551-9175570 None:intergenic 20.0%
!! ATTTCAAAATATCGATCTAA+TGG - chr2.1:9175901-9175920 MS.gene68646:CDS 20.0%
!! GATATTATATTTCAAATGTC+GGG - chr2.1:9176110-9176129 MS.gene68646:intron 20.0%
!! TTACGATCATATTATACATT+TGG - chr2.1:9174372-9174391 MS.gene68646:CDS 20.0%
!!! AGTTTTATGTCACTATATTA+TGG - chr2.1:9174844-9174863 MS.gene68646:CDS 20.0%
!!! ATAATTTGTGTAACTAACAT+TGG + chr2.1:9175106-9175125 None:intergenic 20.0%
!!! TTCTTTTGTCTATTTGTAAA+TGG - chr2.1:9175838-9175857 MS.gene68646:intron 20.0%
! AAAATGACATTATGGTTGTT+GGG - chr2.1:9175781-9175800 MS.gene68646:intron 25.0%
! AAACTTAACTTTCACTCATT+AGG - chr2.1:9175144-9175163 MS.gene68646:CDS 25.0%
! ATAGGATAGAATGATTCAAT+AGG + chr2.1:9176150-9176169 None:intergenic 25.0%
! ATGTTGTAGAATACAGATAA+TGG + chr2.1:9174207-9174226 None:intergenic 25.0%
! ATTGTATATTACATATGCGT+TGG + chr2.1:9175599-9175618 None:intergenic 25.0%
! ATTTAGTTATCTCGTACATT+TGG + chr2.1:9174699-9174718 None:intergenic 25.0%
! CTATCCTATGTAACATATTA+TGG - chr2.1:9176161-9176180 MS.gene68646:CDS 25.0%
! CTTCTTCTTGTGTATATATA+TGG + chr2.1:9176261-9176280 None:intergenic 25.0%
! TAAATGAAACATTCTCTAGT+TGG + chr2.1:9175263-9175282 None:intergenic 25.0%
! TAATGAGTGAAAGTTAAGTT+TGG + chr2.1:9175145-9175164 None:intergenic 25.0%
! TATATATACACAAGAAGAAG+TGG - chr2.1:9176261-9176280 MS.gene68646:intron 25.0%
! TCATCCATAATATGTTACAT+AGG + chr2.1:9176168-9176187 None:intergenic 25.0%
! TGAAACAGAAATATTGTGAT+TGG - chr2.1:9173500-9173519 MS.gene68646:CDS 25.0%
!! ACTATTTGGATTTTCCTTAT+CGG - chr2.1:9175452-9175471 MS.gene68646:intron 25.0%
!!! TTTCTTGTTTTGCAAAATGT+TGG - chr2.1:9173971-9173990 MS.gene68646:intron 25.0%
AAATGACATTATGGTTGTTG+GGG - chr2.1:9175782-9175801 MS.gene68646:intron 30.0%
AAATTTCGCCATATTTATCG+AGG - chr2.1:9173473-9173492 MS.gene68646:CDS 30.0%
AACATTCTCTAGTTGGAAAT+TGG + chr2.1:9175256-9175275 None:intergenic 30.0%
ACAGAAATATTGTGATTGGT+TGG - chr2.1:9173504-9173523 MS.gene68646:CDS 30.0%
ACTTTGTGTATATCACCATA+TGG + chr2.1:9175198-9175217 None:intergenic 30.0%
AGAAATGAAGTTGGAATATC+AGG - chr2.1:9175312-9175331 MS.gene68646:intron 30.0%
CATACTAATCTTGCATCAAA+TGG - chr2.1:9174916-9174935 MS.gene68646:CDS 30.0%
GAAAATCCAAATAGTCTACA+AGG + chr2.1:9175447-9175466 None:intergenic 30.0%
GAAAATGACATTATGGTTGT+TGG - chr2.1:9175780-9175799 MS.gene68646:intron 30.0%
GTAAATCCTTGTAGACTATT+TGG - chr2.1:9175438-9175457 MS.gene68646:intron 30.0%
TATATCACCATATGGAAAAG+AGG + chr2.1:9175190-9175209 None:intergenic 30.0%
TATGGTGATATACACAAAGT+TGG - chr2.1:9175198-9175217 MS.gene68646:CDS 30.0%
TATTTAATGGCTGAAACAGA+TGG + chr2.1:9175872-9175891 None:intergenic 30.0%
TGACAGTGAAAATGACATTA+TGG - chr2.1:9175773-9175792 MS.gene68646:intron 30.0%
TGATCTAAACCTTAACTCTT+GGG - chr2.1:9175074-9175093 MS.gene68646:intron 30.0%
TTAGGACACAATAAGTTTAG+TGG + chr2.1:9174730-9174749 None:intergenic 30.0%
TTGATCTAAACCTTAACTCT+TGG - chr2.1:9175073-9175092 MS.gene68646:intron 30.0%
TTGATTAAGCTCTCATCATT+TGG + chr2.1:9174479-9174498 None:intergenic 30.0%
TTTAGTAAAACTTACTGCCA+TGG + chr2.1:9175813-9175832 None:intergenic 30.0%
! AATGGTTTTCTAACTTACAC+TGG + chr2.1:9176012-9176031 None:intergenic 30.0%
! ATATCATTTTCATGTCTTGC+AGG - chr2.1:9174228-9174247 MS.gene68646:intron 30.0%
! CAAAATTCCTCTTTTCCATA+TGG - chr2.1:9175180-9175199 MS.gene68646:CDS 30.0%
! CTTTTAGCATACTTTTGGTA+AGG + chr2.1:9174081-9174100 None:intergenic 30.0%
! TGATGCTTTTAGCATACTTT+TGG + chr2.1:9174086-9174105 None:intergenic 30.0%
!! AAAAGTATGCTAAAAGCATC+AGG - chr2.1:9174085-9174104 MS.gene68646:CDS 30.0%
!! AACATGAAACATGCTTTTTG+CGG + chr2.1:9173915-9173934 None:intergenic 30.0%
AAGATTGAATGTTCAAGGGA+GGG + chr2.1:9174605-9174624 None:intergenic 35.0%
AAGTGGAGAAGATGACAATT+GGG - chr2.1:9176278-9176297 MS.gene68646:intron 35.0%
AATATATCCATTGAGATCCC+TGG - chr2.1:9175041-9175060 MS.gene68646:intron 35.0%
ACCTGACAAAGATTACAACA+TGG + chr2.1:9173563-9173582 None:intergenic 35.0%
AGCTATCTGTGATATTACAC+AGG - chr2.1:9174503-9174522 MS.gene68646:CDS 35.0%
AGTGAACTAGAGAGACTATT+TGG - chr2.1:9175699-9175718 MS.gene68646:CDS 35.0%
ATAAATGGCCCAAGTCAAAA+TGG - chr2.1:9174294-9174313 MS.gene68646:CDS 35.0%
CGACAAGATTGAATGTTCAA+GGG + chr2.1:9174609-9174628 None:intergenic 35.0%
GAAAACGGAGAGTTAAGATT+GGG - chr2.1:9173788-9173807 MS.gene68646:intron 35.0%
GAATGAAGGTTCACAAACAA+GGG - chr2.1:9175619-9175638 MS.gene68646:intron 35.0%
GATCTAAACCTTAACTCTTG+GGG - chr2.1:9175075-9175094 MS.gene68646:intron 35.0%
TAAGTTTGGTTGCATTATGC+AGG + chr2.1:9175131-9175150 None:intergenic 35.0%
TCCAACAGTAGTTAGAATCT+AGG - chr2.1:9176393-9176412 MS.gene68646:CDS 35.0%
TCCATGTTGTAATCTTTGTC+AGG - chr2.1:9173559-9173578 MS.gene68646:CDS 35.0%
TCGACAAGATTGAATGTTCA+AGG + chr2.1:9174610-9174629 None:intergenic 35.0%
TGAAAACGGAGAGTTAAGAT+TGG - chr2.1:9173787-9173806 MS.gene68646:intron 35.0%
TGAACATTCAATCTTGTCGA+AGG - chr2.1:9174610-9174629 MS.gene68646:CDS 35.0%
TGAATGAAGGTTCACAAACA+AGG - chr2.1:9175618-9175637 MS.gene68646:intron 35.0%
TGACAAAAATTTCAACGCGA+AGG - chr2.1:9175416-9175435 MS.gene68646:intron 35.0%
TGATGATAACCAGAGTTGTT+TGG - chr2.1:9176309-9176328 MS.gene68646:intron 35.0%
TTCAATAGGACAACTCATTC+AGG + chr2.1:9176136-9176155 None:intergenic 35.0%
TTCACCTACCTCGATAAATA+TGG + chr2.1:9173484-9173503 None:intergenic 35.0%
TTCTCTAGTTGGAAATTGGT+TGG + chr2.1:9175252-9175271 None:intergenic 35.0%
TTGGATTCATACCAGTGATA+AGG + chr2.1:9174680-9174699 None:intergenic 35.0%
! AGAGTTCTGTAGATTTTGTG+AGG + chr2.1:9175490-9175509 None:intergenic 35.0%
! AGCCAAAAGAATCTTGTTTC+TGG - chr2.1:9173628-9173647 MS.gene68646:intron 35.0%
! ATTTGGATTTTCCTTATCGG+CGG - chr2.1:9175455-9175474 MS.gene68646:intron 35.0%
! CATTTTGAATCTTGTCCCTA+TGG + chr2.1:9174121-9174140 None:intergenic 35.0%
! GATATACACAAAGTTGGTCA+AGG - chr2.1:9175204-9175223 MS.gene68646:CDS 35.0%
! GCCAAAAGAATCTTGTTTCT+GGG - chr2.1:9173629-9173648 MS.gene68646:intron 35.0%
! GTGATTTGGATCCCTATAAA+TGG - chr2.1:9174279-9174298 MS.gene68646:intron 35.0%
! TAGTTGGAAATTGGTTGGTT+TGG + chr2.1:9175247-9175266 None:intergenic 35.0%
! TGAACTGAGAGCTTTTGAAT+TGG - chr2.1:9173686-9173705 MS.gene68646:intron 35.0%
! TGGTTACCAACAATTGATTC+AGG + chr2.1:9173848-9173867 None:intergenic 35.0%
! TTCATGTTTTCTACAGTCCA+AGG - chr2.1:9173927-9173946 MS.gene68646:intron 35.0%
! TTCTAGTTATGCAGACTTCA+CGG - chr2.1:9174981-9175000 MS.gene68646:CDS 35.0%
!! AAGGTGCATCCGTTTTTAAT+TGG + chr2.1:9175952-9175971 None:intergenic 35.0%
!! CCAAAAGAATCTTGTTTCTG+GGG - chr2.1:9173630-9173649 MS.gene68646:intron 35.0%
!! TCCGTTTTTAATTGGTGTAC+CGG + chr2.1:9175944-9175963 None:intergenic 35.0%
!! TTTGACTTGGGCCATTTATA+GGG + chr2.1:9174293-9174312 None:intergenic 35.0%
!!! AAGCATCTCCATTTTGACTT+GGG + chr2.1:9174305-9174324 None:intergenic 35.0%
!!! TTTTGACTTGGGCCATTTAT+AGG + chr2.1:9174294-9174313 None:intergenic 35.0%
AATACTCCTTCAGCTCAGAT+AGG - chr2.1:9175282-9175301 MS.gene68646:intron 40.0%
ACTACTGTTGGAGAAGAATC+TGG + chr2.1:9176385-9176404 None:intergenic 40.0%
ACTAGAGAGACTATTTGGCA+TGG - chr2.1:9175704-9175723 MS.gene68646:CDS 40.0%
AGTGACTGGAATGAGTGATT+TGG - chr2.1:9174265-9174284 MS.gene68646:intron 40.0%
ATACCAGTGATAAGGTGACT+GGG + chr2.1:9174672-9174691 None:intergenic 40.0%
ATAGAGATCCATGAGCAGTA+AGG + chr2.1:9173760-9173779 None:intergenic 40.0%
ATCAAGATAGAGTCTCTCCT+TGG - chr2.1:9174556-9174575 MS.gene68646:CDS 40.0%
ATTGAGCAGCTACAATGATC+TGG - chr2.1:9175674-9175693 MS.gene68646:CDS 40.0%
CAAGATTGAATGTTCAAGGG+AGG + chr2.1:9174606-9174625 None:intergenic 40.0%
CCCGGTACACCAATTAAAAA+CGG - chr2.1:9175940-9175959 MS.gene68646:CDS 40.0%
CTTGTTGCAAAAGACCTGAA+TGG - chr2.1:9173440-9173459 MS.gene68646:CDS 40.0%
GAAGTGGAGAAGATGACAAT+TGG - chr2.1:9176277-9176296 MS.gene68646:intron 40.0%
GCTCAATCTTGAGAGATCAA+TGG + chr2.1:9175661-9175680 None:intergenic 40.0%
GGTCTTCCTGAATCAATTGT+TGG - chr2.1:9173839-9173858 MS.gene68646:intron 40.0%
GGTGCATTGAGAAATGAAGT+TGG - chr2.1:9175303-9175322 MS.gene68646:intron 40.0%
GTCAAAATGGAGATGCTTGA+TGG - chr2.1:9174307-9174326 MS.gene68646:CDS 40.0%
GTGAAGTCTGCATAACTAGA+AGG + chr2.1:9174982-9175001 None:intergenic 40.0%
GTGTATGTTCCAAACAACTC+TGG + chr2.1:9176321-9176340 None:intergenic 40.0%
GTTATAGCTGACCTTCTTTC+TGG + chr2.1:9174165-9174184 None:intergenic 40.0%
TAAGAGATCCTGACAAAGGA+TGG - chr2.1:9175736-9175755 MS.gene68646:CDS 40.0%
TACTTCTTCCGAGTTTACGA+GGG - chr2.1:9174951-9174970 MS.gene68646:CDS 40.0%
TAGCATTAGCCACTGAAGAA+AGG + chr2.1:9173889-9173908 None:intergenic 40.0%
TATCCGCTAATCAAGTACCT+TGG + chr2.1:9173947-9173966 None:intergenic 40.0%
TCAAGATAGAGTCTCTCCTT+GGG - chr2.1:9174557-9174576 MS.gene68646:CDS 40.0%
TCCTAGTTTCCTTTCTTCAG+TGG - chr2.1:9173877-9173896 MS.gene68646:intron 40.0%
TCTCTATGTAGGGGTGAAAA+CGG - chr2.1:9173773-9173792 MS.gene68646:intron 40.0%
TGAGTCTATAAGATCGACCA+AGG - chr2.1:9174798-9174817 MS.gene68646:CDS 40.0%
TGATATTACACAGGTCCGAT+GGG - chr2.1:9174512-9174531 MS.gene68646:CDS 40.0%
TGCTAAAAGAAGTTGCACGA+AGG - chr2.1:9175509-9175528 MS.gene68646:CDS 40.0%
TGCTCATGGATCTCTATGTA+GGG - chr2.1:9173763-9173782 MS.gene68646:intron 40.0%
TGGATGAATCTCCAGAAAGA+AGG - chr2.1:9174151-9174170 MS.gene68646:CDS 40.0%
TTACTTCTTCCGAGTTTACG+AGG - chr2.1:9174950-9174969 MS.gene68646:CDS 40.0%
TTCGCCATATTTATCGAGGT+AGG - chr2.1:9173477-9173496 MS.gene68646:CDS 40.0%
TTGGTAAGGCACAACGAAAT+CGG + chr2.1:9174067-9174086 None:intergenic 40.0%
TTTGCAACAAGCTCTTGAGA+GGG + chr2.1:9173431-9173450 None:intergenic 40.0%
! ACATACACCATTGATTGTGG+AGG - chr2.1:9176333-9176352 MS.gene68646:CDS 40.0%
! AGGATTTTCCATCCTTTGTC+AGG + chr2.1:9175747-9175766 None:intergenic 40.0%
! AGTCCAAGGTACTTGATTAG+CGG - chr2.1:9173941-9173960 MS.gene68646:intron 40.0%
! ATGCGTTGGCAAATTGTCAA+AGG + chr2.1:9175585-9175604 None:intergenic 40.0%
! CATTTTCACTGTCAGTGTAG+AGG + chr2.1:9175767-9175786 None:intergenic 40.0%
! CCCCAGAAACAAGATTCTTT+TGG + chr2.1:9173633-9173652 None:intergenic 40.0%
! CTTCTAAGAGATCCTGACAA+AGG - chr2.1:9175732-9175751 MS.gene68646:CDS 40.0%
! GGAACATACACCATTGATTG+TGG - chr2.1:9176330-9176349 MS.gene68646:CDS 40.0%
! GTTCTGTAGATTTTGTGAGG+TGG + chr2.1:9175487-9175506 None:intergenic 40.0%
! TTTGAAGCCTCCACAATCAA+TGG + chr2.1:9176343-9176362 None:intergenic 40.0%
! TTTTGCAACAAGCTCTTGAG+AGG + chr2.1:9173432-9173451 None:intergenic 40.0%
!! CCGTTTTTAATTGGTGTACC+GGG + chr2.1:9175943-9175962 None:intergenic 40.0%
!! TGGTTTTACCCCAAGAGTTA+AGG + chr2.1:9175086-9175105 None:intergenic 40.0%
!!! CAAGCATCTCCATTTTGACT+TGG + chr2.1:9174306-9174325 None:intergenic 40.0%
AATCTTGTCCCTATGGTCAG+TGG + chr2.1:9174114-9174133 None:intergenic 45.0%
AATCTTTGTCAGGTCAGCCA+AGG - chr2.1:9173569-9173588 MS.gene68646:CDS 45.0%
AATGCACCTATCTGAGCTGA+AGG + chr2.1:9175291-9175310 None:intergenic 45.0%
ACAGCAGACAATCGAAGTCA+AGG + chr2.1:9175971-9175990 None:intergenic 45.0%
AGAACTCACTGCAGGCAAAA+GGG + chr2.1:9176219-9176238 None:intergenic 45.0%
ATCTTGTCCCTATGGTCAGT+GGG + chr2.1:9174113-9174132 None:intergenic 45.0%
ATGTCTTGCAGGTGTAGTAG+CGG - chr2.1:9174239-9174258 MS.gene68646:intron 45.0%
ATTTCCACTCCACACCATTC+AGG + chr2.1:9173457-9173476 None:intergenic 45.0%
CACAAACAAGGGAGCTTAGT+TGG - chr2.1:9175630-9175649 MS.gene68646:intron 45.0%
CATAACACAGAACTCACTGC+AGG + chr2.1:9176227-9176246 None:intergenic 45.0%
CATACCAGTGATAAGGTGAC+TGG + chr2.1:9174673-9174692 None:intergenic 45.0%
CCTCAATATCCTCATCCCAT+CGG + chr2.1:9174530-9174549 None:intergenic 45.0%
CTGCAGTGAGTTCTGTGTTA+TGG - chr2.1:9176225-9176244 MS.gene68646:intron 45.0%
CTGCTCATGGATCTCTATGT+AGG - chr2.1:9173762-9173781 MS.gene68646:intron 45.0%
CTTCTCTAGGTGCAAACTTG+AGG - chr2.1:9175385-9175404 MS.gene68646:intron 45.0%
GAGAGACTATTTGGCATGGA+AGG - chr2.1:9175708-9175727 MS.gene68646:CDS 45.0%
GATTCAGGAAGACCGTTTCT+CGG + chr2.1:9173833-9173852 None:intergenic 45.0%
GCCACTGAAGAAAGGAAACT+AGG + chr2.1:9173881-9173900 None:intergenic 45.0%
GCTCATGGATCTCTATGTAG+GGG - chr2.1:9173764-9173783 MS.gene68646:intron 45.0%
GCTTCAAAGTCTTCCTCAGT+GGG - chr2.1:9176355-9176374 MS.gene68646:CDS 45.0%
GGAAACTAGGAGAACAACTC+TGG + chr2.1:9173868-9173887 None:intergenic 45.0%
GGCTATGACTTATGTTGTCC+CGG - chr2.1:9175922-9175941 MS.gene68646:CDS 45.0%
GTCGTCGGATCATCATTCAA+TGG - chr2.1:9176063-9176082 MS.gene68646:intron 45.0%
GTGATATTACACAGGTCCGA+TGG - chr2.1:9174511-9174530 MS.gene68646:CDS 45.0%
GTTCATCCAGAATGAGTCGT+CGG - chr2.1:9176048-9176067 MS.gene68646:intron 45.0%
TAGATCAACCTTTCCTGCCA+GGG + chr2.1:9175061-9175080 None:intergenic 45.0%
TGCAAAAGACCTGAATGGTG+TGG - chr2.1:9173445-9173464 MS.gene68646:CDS 45.0%
TGTAGTAGCGGTATAGTGAC+TGG - chr2.1:9174251-9174270 MS.gene68646:intron 45.0%
TTAGATCAACCTTTCCTGCC+AGG + chr2.1:9175062-9175081 None:intergenic 45.0%
TTCTTGACCTTGCAAGACCT+TGG + chr2.1:9174818-9174837 None:intergenic 45.0%
! ATTTCTGCTGCTGCTTCTCT+AGG - chr2.1:9175372-9175391 MS.gene68646:intron 45.0%
! TAGAAAGGCGTACGCTTCAA+CGG + chr2.1:9174432-9174451 None:intergenic 45.0%
AAGACCTGAATGGTGTGGAG+TGG - chr2.1:9173450-9173469 MS.gene68646:CDS 50.0%
AAGGCTGAAGAAACTGCGGA+CGG - chr2.1:9174629-9174648 MS.gene68646:CDS 50.0%
AAGGGAGGGAGAGAAGTAGA+CGG + chr2.1:9174591-9174610 None:intergenic 50.0%
AGATCGACCAAGGTCTTGCA+AGG - chr2.1:9174808-9174827 MS.gene68646:CDS 50.0%
AGGCTGAACCCTCGTAAACT+CGG + chr2.1:9174962-9174981 None:intergenic 50.0%
ATCAGGAACCCACTGACCAT+AGG - chr2.1:9174102-9174121 MS.gene68646:CDS 50.0%
ATTGAGATCCCTGGCAGGAA+AGG - chr2.1:9175050-9175069 MS.gene68646:intron 50.0%
CAGAACTCACTGCAGGCAAA+AGG + chr2.1:9176220-9176239 None:intergenic 50.0%
CAGACTTCACGGAAACGAAC+CGG - chr2.1:9174992-9175011 MS.gene68646:CDS 50.0%
CCGATGGGATGAGGATATTG+AGG - chr2.1:9174527-9174546 MS.gene68646:CDS 50.0%
CCTGCAGCTTCTGCTTGTTT+CGG + chr2.1:9175347-9175366 None:intergenic 50.0%
CTTGCAAGACCCTAGGAAAC+CGG + chr2.1:9175014-9175033 None:intergenic 50.0%
GATGATCCGACGACTCATTC+TGG + chr2.1:9176057-9176076 None:intergenic 50.0%
GCAGTGTACCTTACTGCTCA+TGG - chr2.1:9173749-9173768 MS.gene68646:intron 50.0%
GCGATGAAGATGGGGTGATT+AGG + chr2.1:9174748-9174767 None:intergenic 50.0%
GGCTTCAAAGTCTTCCTCAG+TGG - chr2.1:9176354-9176373 MS.gene68646:CDS 50.0%
GTCGAAGGCTGAAGAAACTG+CGG - chr2.1:9174625-9174644 MS.gene68646:CDS 50.0%
TATCCATTGAGATCCCTGGC+AGG - chr2.1:9175045-9175064 MS.gene68646:intron 50.0%
TCACCCAGTCACCTTATCAC+TGG - chr2.1:9174666-9174685 MS.gene68646:CDS 50.0%
TCAGGAACCCACTGACCATA+GGG - chr2.1:9174103-9174122 MS.gene68646:CDS 50.0%
TCTCCAGCTGCGATGAAGAT+GGG + chr2.1:9174757-9174776 None:intergenic 50.0%
TCTTGACCTTGCAAGACCCT+AGG + chr2.1:9175021-9175040 None:intergenic 50.0%
TGAGCAGTAAGGTACACTGC+AGG + chr2.1:9173749-9173768 None:intergenic 50.0%
TGGTTGTTGGGGATGATCCA+TGG - chr2.1:9175793-9175812 MS.gene68646:intron 50.0%
TTTCCTGCCAGGGATCTCAA+TGG + chr2.1:9175051-9175070 None:intergenic 50.0%
! AGCTGGAGACAGTGGGTTTA+TGG - chr2.1:9174768-9174787 MS.gene68646:CDS 50.0%
! GGAAACGAACCGGTTTCCTA+GGG - chr2.1:9175002-9175021 MS.gene68646:CDS 50.0%
! TGGATTTTCCTTATCGGCGG+CGG - chr2.1:9175458-9175477 MS.gene68646:intron 50.0%
AGAATCTGGCTGTCCCACTG+AGG + chr2.1:9176371-9176390 None:intergenic 55.0%
AGAGCTGCTCGACCGAGAAA+CGG - chr2.1:9173818-9173837 MS.gene68646:intron 55.0%
AGGCTGAAGAAACTGCGGAC+GGG - chr2.1:9174630-9174649 MS.gene68646:CDS 55.0%
CCGAAACAAGCAGAAGCTGC+AGG - chr2.1:9175344-9175363 MS.gene68646:intron 55.0%
CGGTTTCCTAGGGTCTTGCA+AGG - chr2.1:9175012-9175031 MS.gene68646:CDS 55.0%
CTCCAGCTGCGATGAAGATG+GGG + chr2.1:9174756-9174775 None:intergenic 55.0%
CTTTGTCAGGTCAGCCAAGG+AGG - chr2.1:9173572-9173591 MS.gene68646:CDS 55.0%
GGGACGCAGTGCTCTTTCTA+AGG - chr2.1:9173650-9173669 MS.gene68646:intron 55.0%
GTACGCTTCAACGGCACAGT+TGG + chr2.1:9174423-9174442 None:intergenic 55.0%
GTAGACGGATCGATCTCCCA+AGG + chr2.1:9174576-9174595 None:intergenic 55.0%
GTAGTGAGCAGATGCCTCCT+TGG + chr2.1:9173589-9173608 None:intergenic 55.0%
GTCTCCAGCTGCGATGAAGA+TGG + chr2.1:9174758-9174777 None:intergenic 55.0%
TACACAGGTCCGATGGGATG+AGG - chr2.1:9174518-9174537 MS.gene68646:CDS 55.0%
TCATCGCAGCTGGAGACAGT+GGG - chr2.1:9174761-9174780 MS.gene68646:CDS 55.0%
TTCATCGCAGCTGGAGACAG+TGG - chr2.1:9174760-9174779 MS.gene68646:CDS 55.0%
! CGGAAACGAACCGGTTTCCT+AGG - chr2.1:9175001-9175020 MS.gene68646:CDS 55.0%
!! AGGAGGCATCTGCTCACTAC+TGG - chr2.1:9173589-9173608 MS.gene68646:intron 55.0%
CACCCCATCTTCATCGCAGC+TGG - chr2.1:9174751-9174770 MS.gene68646:CDS 60.0%
GGCATCTGCTCACTACTGGC+TGG - chr2.1:9173593-9173612 MS.gene68646:intron 60.0%
GGTGGTTTCCGCCGCCGATA+AGG + chr2.1:9175469-9175488 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 9173410 9176414 9173410 ID=MS.gene68646
chr2.1 mRNA 9173410 9176414 9173410 ID=MS.gene68646.t1;Parent=MS.gene68646
chr2.1 exon 9176330 9176414 9176330 ID=MS.gene68646.t1.exon1;Parent=MS.gene68646.t1
chr2.1 CDS 9176330 9176414 9176330 ID=cds.MS.gene68646.t1;Parent=MS.gene68646.t1
chr2.1 exon 9176153 9176243 9176153 ID=MS.gene68646.t1.exon2;Parent=MS.gene68646.t1
chr2.1 CDS 9176153 9176243 9176153 ID=cds.MS.gene68646.t1;Parent=MS.gene68646.t1
chr2.1 exon 9175876 9176040 9175876 ID=MS.gene68646.t1.exon3;Parent=MS.gene68646.t1
chr2.1 CDS 9175876 9176040 9175876 ID=cds.MS.gene68646.t1;Parent=MS.gene68646.t1
chr2.1 exon 9175652 9175771 9175652 ID=MS.gene68646.t1.exon4;Parent=MS.gene68646.t1
chr2.1 CDS 9175652 9175771 9175652 ID=cds.MS.gene68646.t1;Parent=MS.gene68646.t1
chr2.1 exon 9175496 9175574 9175496 ID=MS.gene68646.t1.exon5;Parent=MS.gene68646.t1
chr2.1 CDS 9175496 9175574 9175496 ID=cds.MS.gene68646.t1;Parent=MS.gene68646.t1
chr2.1 exon 9175137 9175299 9175137 ID=MS.gene68646.t1.exon6;Parent=MS.gene68646.t1
chr2.1 CDS 9175137 9175299 9175137 ID=cds.MS.gene68646.t1;Parent=MS.gene68646.t1
chr2.1 exon 9174294 9175054 9174294 ID=MS.gene68646.t1.exon7;Parent=MS.gene68646.t1
chr2.1 CDS 9174294 9175054 9174294 ID=cds.MS.gene68646.t1;Parent=MS.gene68646.t1
chr2.1 exon 9174007 9174197 9174007 ID=MS.gene68646.t1.exon8;Parent=MS.gene68646.t1
chr2.1 CDS 9174007 9174197 9174007 ID=cds.MS.gene68646.t1;Parent=MS.gene68646.t1
chr2.1 exon 9173410 9173593 9173410 ID=MS.gene68646.t1.exon9;Parent=MS.gene68646.t1
chr2.1 CDS 9173410 9173593 9173410 ID=cds.MS.gene68646.t1;Parent=MS.gene68646.t1
Gene Sequence

>MS.gene68646

GATTATAAGCAGCAGAGACCCTCTCAAGAGCTTGTTGCAAAAGACCTGAATGGTGTGGAGTGGAAATTTCGCCATATTTATCGAGGTAGGTGAAACAGAAATATTGTGATTGGTTGGATTCAAATATTGACAATGAATGAAACAGAAATTCCATGTTGTAATCTTTGTCAGGTCAGCCAAGGAGGCATCTGCTCACTACTGGCTGGAGTGCTTTTGTTAGCCAAAAGAATCTTGTTTCTGGGGACGCAGTGCTCTTTCTAAGGTGTGTGTTTGTGTTGAACTGAGAGCTTTTGAATTGGCATTAATCTGTATATGCTAGATATTTCATATGTTGTTCCTGCAGTGTACCTTACTGCTCATGGATCTCTATGTAGGGGTGAAAACGGAGAGTTAAGATTGGGAATCAGAAGAGCTGCTCGACCGAGAAACGGTCTTCCTGAATCAATTGTTGGTAACCAGAGTTGTTCTCCTAGTTTCCTTTCTTCAGTGGCTAATGCTATCTCCGCAAAAAGCATGTTTCATGTTTTCTACAGTCCAAGGTACTTGATTAGCGGATACAGTTTTCTTGTTTTGCAAAATGTTGGATAAAGAGTTATTTTATCCCACATGTATATTTAATATGTTTGAATTTGATGTAATACAGAGCTAGTCATGCCGATTTCGTTGTGCCTTACCAAAAGTATGCTAAAAGCATCAGGAACCCACTGACCATAGGGACAAGATTCAAAATGAAATTTGAAATGGATGAATCTCCAGAAAGAAGGTCAGCTATAACATTAGTTATACATGTTTAGCCATTATCTGTATTCTACAACATGATATCATTTTCATGTCTTGCAGGTGTAGTAGCGGTATAGTGACTGGAATGAGTGATTTGGATCCCTATAAATGGCCCAAGTCAAAATGGAGATGCTTGATGGTATATTTTCTACTTTCTTTATACACTAATACTATGCAGTATGTTACGATCATATTATACATTTGGTATTTGCTATAGATATTTATCATCACCAACTGTGCCGTTGAAGCGTACGCCTTTCTAAACATTACATTAATATATTAATAACCAAATGATGAGAGCTTAATCAATAAAAGCTATCTGTGATATTACACAGGTCCGATGGGATGAGGATATTGAGGCTAATCATCAAGATAGAGTCTCTCCTTGGGAGATCGATCCGTCTACTTCTCTCCCTCCCTTGAACATTCAATCTTGTCGAAGGCTGAAGAAACTGCGGACGGGTCTGCAAGTTGAATCACCCAGTCACCTTATCACTGGTATGAATCCAAATGTACGAGATAACTAAATAACATTAACCACTAAACTTATTGTGTCCTAATCACCCCATCTTCATCGCAGCTGGAGACAGTGGGTTTATGGACTTTGATGAGTCTATAAGATCGACCAAGGTCTTGCAAGGTCAAGAAAAAACAAGTTTTATGTCACTATATTATGGATGTGACACAGTAACTAAGCAGAAAGAATTTGATATCAATTCTCTAAGACATACTAATCTTGCATCAAATGGAGCAAGAAAGATTACTTCTTCCGAGTTTACGAGGGTTCAGCCTTCTAGTTATGCAGACTTCACGGAAACGAACCGGTTTCCTAGGGTCTTGCAAGGTCAAGAAATATATCCATTGAGATCCCTGGCAGGAAAGGTTGATCTAAACCTTAACTCTTGGGGTAAAACCAATGTTAGTTACACAAATTATAACCTGCATAATGCAACCAAACTTAACTTTCACTCATTAGGATCAGAAGTTCTTCAAAATTCCTCTTTTCCATATGGTGATATACACAAAGTTGGTCAAGGTAGCAGCATGCTGTGCTCCAAACCAACCAATTTCCAACTAGAGAATGTTTCATTTAATACTCCTTCAGCTCAGATAGGTGCATTGAGAAATGAAGTTGGAATATCAGGTTTCACAATCCGAAACAAGCAGAAGCTGCAGGACAATATTTCTGCTGCTGCTTCTCTAGGTGCAAACTTGAGGATTTCAAATGACAAAAATTTCAACGCGAAGGTAAATCCTTGTAGACTATTTGGATTTTCCTTATCGGCGGCGGAAACCACCTCACAAAATCTACAGAACTCTGCTAAAAGAAGTTGCACGAAGGTGAGCGACGATAGCAACCGAGTTATATTGTGATATGAATTTTTCTCTATTCCTTTGACAATTTGCCAACGCATATGTAATATACAATGAATGAAGGTTCACAAACAAGGGAGCTTAGTTGGAAGAGCCATTGATCTCTCAAGATTGAGCAGCTACAATGATCTGGTGAGTGAACTAGAGAGACTATTTGGCATGGAAGGTCTTCTAAGAGATCCTGACAAAGGATGGAAAATCCTCTACACTGACAGTGAAAATGACATTATGGTTGTTGGGGATGATCCATGGCAGTAAGTTTTACTAAAAATGCTTCTTTTGTCTATTTGTAAATGGTATACACACCATCTGTTTCAGCCATTAAATAATTATAAATATTTCAAAATATCGATCTAATGGCTATGACTTATGTTGTCCCGGTACACCAATTAAAAACGGATGCACCTTGACTTCGATTGTCTGCTGTAACAATATCACACATTCACCAGTGTAAGTTAGAAAACCATTGAATAAATATCATATGGTTCATCCAGAATGAGTCGTCGGATCATCATTCAATGGTTTTTTTGACTGACTGTGCCTTGTGATATTATATTTCAAATGTCGGGCCTGAATGAGTTGTCCTATTGAATCATTCTATCCTATGTAACATATTATGGATGATATGATACATACTGAAAAAATACACTCACCCTTTTGCCTGCAGTGAGTTCTGTGTTATGGTATCAAAAATCCATATATATACACAAGAAGAAGTGGAGAAGATGACAATTGGGATGATGAATGATGATAACCAGAGTTGTTTGGAACATACACCATTGATTGTGGAGGCTTCAAAGTCTTCCTCAGTGGGACAGCCAGATTCTTCTCCAACAGTAGTTAGAATCTAG
Protein sequence

>MS.gene68646.t1

DYKQQRPSQELVAKDLNGVEWKFRHIYRGQPRRHLLTTGWSAFVSQKNLVSGDAVLFLRGENGELRLGIRRAARPRNGLPESIVGNQSCSPSFLSSVANAISAKSMFHVFYSPRASHADFVVPYQKYAKSIRNPLTIGTRFKMKFEMDESPERRCSSGIVTGMSDLDPYKWPKSKWRCLMVRWDEDIEANHQDRVSPWEIDPSTSLPPLNIQSCRRLKKLRTGLQVESPSHLITAGDSGFMDFDESIRSTKVLQGQEKTSFMSLYYGCDTVTKQKEFDINSLRHTNLASNGARKITSSEFTRVQPSSYADFTETNRFPRVLQGQEIYPLRSLAGKVDLNLNSWGKTNVSYTNYNLHNATKLNFHSLGSEVLQNSSFPYGDIHKVGQGSSMLCSKPTNFQLENVSFNTPSAQIGALRNEVGISGFTIRNKQKLQDNISAAASLGANLRISNDKNFNAKVNPCRLFGFSLSAAETTSQNLQNSAKRSCTKVHKQGSLVGRAIDLSRLSSYNDLVSELERLFGMEGLLRDPDKGWKILYTDSENDIMVVGDDPWHEFCVMVSKIHIYTQEEVEKMTIGMMNDDNQSCLEHTPLIVEASKSSSVGQPDSSPTVVRI